Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43937.t1 | XP_013453735.1 | 91 | 133 | 12 | 0 | 2 | 134 | 247 | 379 | 2.20E-63 | 251.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43937.t1 | A0A072UEA2 | 91.0 | 133 | 12 | 0 | 2 | 134 | 247 | 379 | 1.6e-63 | 251.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049413 | MS.gene43937 | 0.815364 | 9.60E-52 | -1.69E-46 |
| MS.gene050961 | MS.gene43937 | 0.807651 | 4.56E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene43937 | 0.80691 | 6.54E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene43937 | 0.806425 | 8.28E-50 | -1.69E-46 |
| MS.gene052493 | MS.gene43937 | 0.809859 | 1.54E-50 | -1.69E-46 |
| MS.gene052537 | MS.gene43937 | 0.806195 | 9.25E-50 | -1.69E-46 |
| MS.gene052570 | MS.gene43937 | 0.820575 | 6.38E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene43937 | 0.805272 | 1.45E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene43937 | 0.849592 | 2.93E-60 | -1.69E-46 |
| MS.gene054893 | MS.gene43937 | 0.844408 | 7.70E-59 | -1.69E-46 |
| MS.gene055229 | MS.gene43937 | 0.8021 | 6.59E-49 | -1.69E-46 |
| MS.gene05624 | MS.gene43937 | 0.801466 | 8.89E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene43937 | 0.800888 | 1.17E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene43937 | 0.819629 | 1.05E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene43937 | -0.811849 | 5.70E-51 | -1.69E-46 |
| MS.gene060257 | MS.gene43937 | 0.814745 | 1.32E-51 | -1.69E-46 |
| MS.gene060259 | MS.gene43937 | 0.80374 | 3.02E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene43937 | 0.828577 | 8.32E-55 | -1.69E-46 |
| MS.gene060413 | MS.gene43937 | 0.821679 | 3.55E-53 | -1.69E-46 |
| MS.gene060522 | MS.gene43937 | 0.810288 | 1.24E-50 | -1.69E-46 |
| MS.gene061160 | MS.gene43937 | 0.801387 | 9.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43937.t1 | MTR_5g035665 | 90.977 | 133 | 12 | 0 | 2 | 134 | 247 | 379 | 5.22e-85 | 253 |
| MS.gene43937.t1 | MTR_5g035750 | 89.474 | 133 | 14 | 0 | 2 | 134 | 255 | 387 | 2.69e-83 | 249 |
| MS.gene43937.t1 | MTR_5g035690 | 87.970 | 133 | 16 | 0 | 2 | 134 | 254 | 386 | 2.92e-81 | 244 |
| MS.gene43937.t1 | MTR_5g035760 | 86.667 | 135 | 16 | 2 | 2 | 134 | 246 | 380 | 7.77e-77 | 232 |
| MS.gene43937.t1 | MTR_5g035670 | 81.481 | 135 | 23 | 1 | 2 | 134 | 247 | 381 | 2.30e-71 | 218 |
| MS.gene43937.t1 | MTR_6g472110 | 51.408 | 142 | 60 | 6 | 2 | 134 | 258 | 399 | 6.59e-36 | 127 |
| MS.gene43937.t1 | MTR_6g016660 | 41.600 | 125 | 68 | 1 | 15 | 134 | 274 | 398 | 1.15e-26 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43937.t1 | AT1G13480 | 45.299 | 117 | 58 | 3 | 23 | 134 | 272 | 387 | 1.49e-25 | 99.8 |
| MS.gene43937.t1 | AT1G13510 | 41.606 | 137 | 73 | 4 | 2 | 134 | 247 | 380 | 3.69e-23 | 93.2 |
| MS.gene43937.t1 | AT1G13500 | 46.316 | 95 | 49 | 2 | 42 | 134 | 292 | 386 | 1.00e-22 | 92.0 |
| MS.gene43937.t1 | AT1G13490 | 43.243 | 111 | 59 | 3 | 27 | 134 | 273 | 382 | 1.47e-22 | 91.3 |
| MS.gene43937.t1 | AT1G13490 | 43.243 | 111 | 59 | 3 | 27 | 134 | 254 | 363 | 1.74e-22 | 90.9 |
| MS.gene43937.t1 | AT1G13530 | 45.263 | 95 | 50 | 2 | 42 | 134 | 291 | 385 | 2.68e-22 | 90.9 |
| MS.gene43937.t1 | AT1G13470 | 46.364 | 110 | 55 | 4 | 27 | 134 | 271 | 378 | 1.21e-21 | 89.0 |
| MS.gene43937.t1 | AT1G13520 | 42.478 | 113 | 58 | 4 | 27 | 134 | 277 | 387 | 5.42e-21 | 87.0 |
| MS.gene43937.t1 | AT1G13540 | 44.792 | 96 | 50 | 2 | 41 | 134 | 287 | 381 | 9.82e-21 | 86.3 |
| MS.gene43937.t1 | AT1G13550 | 41.818 | 110 | 61 | 3 | 24 | 131 | 277 | 385 | 2.87e-20 | 85.1 |
| MS.gene43937.t1 | AT1G61600 | 34.307 | 137 | 64 | 3 | 24 | 134 | 285 | 421 | 2.06e-13 | 66.2 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGACACTCACATCAAATT+AGG | 0.281648 | 5.4:+40699869 | MS.gene43937:CDS |
| CACATCAAATTAGGAGCAAA+TGG | 0.354134 | 5.4:+40699878 | MS.gene43937:CDS |
| TGAAGGAAAGGACGCTGTTT+GGG | 0.368363 | 5.4:+40699585 | MS.gene43937:CDS |
| TTGAAGGAAAGGACGCTGTT+TGG | 0.369269 | 5.4:+40699584 | MS.gene43937:CDS |
| ACAAAAGTTGAAGAGTTTGA+TGG | 0.392275 | 5.4:+40699754 | MS.gene43937:CDS |
| AAATGGGAACAAAGAAGGTT+TGG | 0.394970 | 5.4:+40699697 | MS.gene43937:CDS |
| AGAAGGTTTGGATGGAATGC+TGG | 0.431581 | 5.4:+40699709 | MS.gene43937:CDS |
| ACAGAAGTTGCTAAGGTTGA+AGG | 0.453239 | 5.4:+40699568 | MS.gene43937:CDS |
| GATGAAAATAGATTGGTTGA+TGG | 0.465731 | 5.4:+40699607 | MS.gene43937:CDS |
| TTTGGATGGAATGCTGGGAA+TGG | 0.475064 | 5.4:+40699715 | MS.gene43937:CDS |
| TCAAAGGAAAATAGTGGAGA+AGG | 0.489041 | 5.4:+40699517 | MS.gene43937:CDS |
| TTTCTATTGAAGAGAATGGA+TGG | 0.489855 | 5.4:+40699823 | MS.gene43937:CDS |
| TGTTTGGGATGAAAATAGAT+TGG | 0.492208 | 5.4:+40699600 | MS.gene43937:CDS |
| CTGAGTTTGGAAGTTGTTGA+GGG | 0.521064 | 5.4:+40699670 | MS.gene43937:CDS |
| GGGAACAAAGAAGGTTTGGA+TGG | 0.521569 | 5.4:+40699701 | MS.gene43937:CDS |
| ACATCAAATTAGGAGCAAAT+GGG | 0.536534 | 5.4:+40699879 | MS.gene43937:CDS |
| GAATGAAATGGGAACAAAGA+AGG | 0.541076 | 5.4:+40699692 | MS.gene43937:CDS |
| TATTCAAACAGAAGTTGCTA+AGG | 0.545070 | 5.4:+40699561 | MS.gene43937:CDS |
| AGTTGTTGAGGGAATGAAAT+GGG | 0.546947 | 5.4:+40699681 | MS.gene43937:CDS |
| GAAGGTTTGGATGGAATGCT+GGG | 0.561735 | 5.4:+40699710 | MS.gene43937:CDS |
| AGTTTGATGGAACAAATAAG+TGG | 0.584146 | 5.4:+40699767 | MS.gene43937:CDS |
| ACTGAGTTTGGAAGTTGTTG+AGG | 0.595211 | 5.4:+40699669 | MS.gene43937:CDS |
| AGTTGCTAAGGTTGAAGGAA+AGG | 0.599670 | 5.4:+40699573 | MS.gene43937:CDS |
| AAGGAAAATAGTGGAGAAGG+TGG | 0.602522 | 5.4:+40699520 | MS.gene43937:CDS |
| AAGTTGTTGAGGGAATGAAA+TGG | 0.611433 | 5.4:+40699680 | MS.gene43937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTCAAAGGAAAATAG+TGG | + | chr5.4:40699511-40699530 | MS.gene43937:CDS | 20.0% |
| ACAAAAGTTGAAGAGTTTGA+TGG | + | chr5.4:40699754-40699773 | MS.gene43937:CDS | 30.0% | |
| ACATCAAATTAGGAGCAAAT+GGG | + | chr5.4:40699879-40699898 | MS.gene43937:CDS | 30.0% | |
| AGTTTGATGGAACAAATAAG+TGG | + | chr5.4:40699767-40699786 | MS.gene43937:CDS | 30.0% | |
| TATTCAAACAGAAGTTGCTA+AGG | + | chr5.4:40699561-40699580 | MS.gene43937:CDS | 30.0% | |
| TTTCTATTGAAGAGAATGGA+TGG | + | chr5.4:40699823-40699842 | MS.gene43937:CDS | 30.0% | |
| ! | GAGTTTTCTATTGAAGAGAA+TGG | + | chr5.4:40699819-40699838 | MS.gene43937:CDS | 30.0% |
| ! | GATGAAAATAGATTGGTTGA+TGG | + | chr5.4:40699607-40699626 | MS.gene43937:CDS | 30.0% |
| ! | TGTTTGGGATGAAAATAGAT+TGG | + | chr5.4:40699600-40699619 | MS.gene43937:CDS | 30.0% |
| !! | AGTGTTGATGAGAAAAATGT+TGG | + | chr5.4:40699646-40699665 | MS.gene43937:CDS | 30.0% |
| AAATGGGAACAAAGAAGGTT+TGG | + | chr5.4:40699697-40699716 | MS.gene43937:CDS | 35.0% | |
| AAGTTGTTGAGGGAATGAAA+TGG | + | chr5.4:40699680-40699699 | MS.gene43937:CDS | 35.0% | |
| AGTTGTTGAGGGAATGAAAT+GGG | + | chr5.4:40699681-40699700 | MS.gene43937:CDS | 35.0% | |
| CACATCAAATTAGGAGCAAA+TGG | + | chr5.4:40699878-40699897 | MS.gene43937:CDS | 35.0% | |
| GAAAAATGTTGGACTGAGTT+TGG | + | chr5.4:40699657-40699676 | MS.gene43937:CDS | 35.0% | |
| GAATGAAATGGGAACAAAGA+AGG | + | chr5.4:40699692-40699711 | MS.gene43937:CDS | 35.0% | |
| TCAGACACTCACATCAAATT+AGG | + | chr5.4:40699869-40699888 | MS.gene43937:CDS | 35.0% | |
| ! | TCAAAGGAAAATAGTGGAGA+AGG | + | chr5.4:40699517-40699536 | MS.gene43937:CDS | 35.0% |
| ACAGAAGTTGCTAAGGTTGA+AGG | + | chr5.4:40699568-40699587 | MS.gene43937:CDS | 40.0% | |
| ACTGAGTTTGGAAGTTGTTG+AGG | + | chr5.4:40699669-40699688 | MS.gene43937:CDS | 40.0% | |
| AGTTGCTAAGGTTGAAGGAA+AGG | + | chr5.4:40699573-40699592 | MS.gene43937:CDS | 40.0% | |
| CTGAGTTTGGAAGTTGTTGA+GGG | + | chr5.4:40699670-40699689 | MS.gene43937:CDS | 40.0% | |
| ! | AAGGAAAATAGTGGAGAAGG+TGG | + | chr5.4:40699520-40699539 | MS.gene43937:CDS | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | + | chr5.4:40699617-40699636 | MS.gene43937:CDS | 40.0% |
| AGAAGGTTTGGATGGAATGC+TGG | + | chr5.4:40699709-40699728 | MS.gene43937:CDS | 45.0% | |
| GAAGGTTTGGATGGAATGCT+GGG | + | chr5.4:40699710-40699729 | MS.gene43937:CDS | 45.0% | |
| GGGAACAAAGAAGGTTTGGA+TGG | + | chr5.4:40699701-40699720 | MS.gene43937:CDS | 45.0% | |
| TGAAGGAAAGGACGCTGTTT+GGG | + | chr5.4:40699585-40699604 | MS.gene43937:CDS | 45.0% | |
| TTGAAGGAAAGGACGCTGTT+TGG | + | chr5.4:40699584-40699603 | MS.gene43937:CDS | 45.0% | |
| TTTGGATGGAATGCTGGGAA+TGG | + | chr5.4:40699715-40699734 | MS.gene43937:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40699502 | 40699906 | 40699502 | ID=MS.gene43937 |
| chr5.4 | mRNA | 40699502 | 40699906 | 40699502 | ID=MS.gene43937.t1;Parent=MS.gene43937 |
| chr5.4 | exon | 40699502 | 40699906 | 40699502 | ID=MS.gene43937.t1.exon1;Parent=MS.gene43937.t1 |
| chr5.4 | CDS | 40699502 | 40699906 | 40699502 | ID=cds.MS.gene43937.t1;Parent=MS.gene43937.t1 |
| Gene Sequence |
| Protein sequence |
