Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44238.t1 | XP_004509365.1 | 86.6 | 246 | 32 | 1 | 1 | 246 | 1 | 245 | 5.90E-115 | 423.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44238.t1 | A0A1P8B554 | 60.0 | 255 | 88 | 6 | 7 | 248 | 7 | 260 | 2.6e-79 | 296.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44238.t1 | A0A1S2YSV9 | 86.6 | 246 | 32 | 1 | 1 | 246 | 1 | 245 | 4.3e-115 | 423.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene44238 | -0.812054 | 5.14E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene44238 | -0.810094 | 1.37E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene44238 | -0.80948 | 1.85E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene44238 | -0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene44238 | -0.823281 | 1.51E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene44238 | -0.80171 | 7.92E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene44238 | -0.808008 | 3.83E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene44238 | -0.813077 | 3.07E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene44238 | -0.807705 | 4.44E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene44238 | -0.818255 | 2.16E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene44238 | -0.80344 | 3.48E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene44238 | -0.804914 | 1.72E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene44238 | -0.803061 | 4.17E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene44238 | 0.817319 | 3.51E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene44238 | -0.800236 | 1.58E-48 | -1.69E-46 |
| MS.gene051874 | MS.gene44238 | -0.814831 | 1.26E-51 | -1.69E-46 |
| MS.gene052164 | MS.gene44238 | -0.825858 | 3.73E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene44238 | -0.8072 | 5.68E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene44238 | -0.802681 | 5.00E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene44238 | -0.815188 | 1.05E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene44238 | -0.818257 | 2.15E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene44238 | -0.809004 | 2.34E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene44238 | -0.800047 | 1.73E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene44238 | -0.830302 | 3.17E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene44238 | -0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene44238 | -0.802482 | 5.49E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene44238 | -0.808609 | 2.85E-50 | -1.69E-46 |
| MS.gene059699 | MS.gene44238 | 0.815621 | 8.42E-52 | -1.69E-46 |
| MS.gene059703 | MS.gene44238 | 0.808427 | 3.11E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene44238 | -0.816146 | 6.43E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene44238 | -0.809399 | 1.93E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene44238 | -0.806252 | 9.00E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene44238 | -0.800818 | 1.21E-48 | -1.69E-46 |
| MS.gene061150 | MS.gene44238 | -0.803355 | 3.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44238.t1 | MTR_8g075510 | 98.785 | 247 | 3 | 0 | 1 | 247 | 1 | 247 | 0.0 | 502 |
| MS.gene44238.t1 | MTR_8g075510 | 98.394 | 249 | 4 | 0 | 1 | 249 | 1 | 249 | 0.0 | 498 |
| MS.gene44238.t1 | MTR_5g022350 | 71.545 | 246 | 69 | 1 | 1 | 246 | 1 | 245 | 2.11e-132 | 376 |
| MS.gene44238.t1 | MTR_4g073720 | 68.644 | 236 | 70 | 2 | 11 | 243 | 5 | 239 | 6.38e-116 | 335 |
| MS.gene44238.t1 | MTR_1g025420 | 61.694 | 248 | 83 | 6 | 14 | 249 | 6 | 253 | 1.38e-99 | 293 |
| MS.gene44238.t1 | MTR_1g025420 | 62.195 | 246 | 83 | 5 | 14 | 249 | 6 | 251 | 1.42e-99 | 291 |
| MS.gene44238.t1 | MTR_3g114030 | 60.606 | 231 | 81 | 5 | 28 | 248 | 4 | 234 | 9.14e-92 | 270 |
| MS.gene44238.t1 | MTR_8g037890 | 56.800 | 250 | 97 | 6 | 10 | 249 | 4 | 252 | 1.16e-91 | 273 |
| MS.gene44238.t1 | MTR_5g022310 | 55.686 | 255 | 97 | 7 | 10 | 249 | 3 | 256 | 2.64e-88 | 262 |
| MS.gene44238.t1 | MTR_8g075550 | 57.872 | 235 | 84 | 5 | 28 | 248 | 25 | 258 | 1.93e-87 | 262 |
| MS.gene44238.t1 | MTR_5g022310 | 55.731 | 253 | 96 | 7 | 10 | 247 | 3 | 254 | 2.44e-87 | 261 |
| MS.gene44238.t1 | MTR_4g073730 | 54.656 | 247 | 96 | 6 | 13 | 244 | 3 | 248 | 5.87e-87 | 260 |
| MS.gene44238.t1 | MTR_6g079580 | 54.357 | 241 | 98 | 6 | 13 | 244 | 3 | 240 | 7.10e-87 | 258 |
| MS.gene44238.t1 | MTR_4g073730 | 54.656 | 247 | 96 | 6 | 13 | 244 | 3 | 248 | 9.65e-87 | 261 |
| MS.gene44238.t1 | MTR_8g036215 | 56.466 | 232 | 92 | 5 | 19 | 243 | 8 | 237 | 2.90e-83 | 249 |
| MS.gene44238.t1 | MTR_7g102380 | 49.383 | 243 | 114 | 4 | 12 | 246 | 2 | 243 | 8.65e-83 | 248 |
| MS.gene44238.t1 | MTR_8g056820 | 53.306 | 242 | 102 | 6 | 15 | 247 | 11 | 250 | 4.47e-81 | 244 |
| MS.gene44238.t1 | MTR_8g107140 | 54.321 | 243 | 100 | 5 | 14 | 248 | 10 | 249 | 5.63e-81 | 243 |
| MS.gene44238.t1 | MTR_3g111620 | 55.466 | 247 | 98 | 7 | 10 | 247 | 6 | 249 | 1.21e-80 | 243 |
| MS.gene44238.t1 | MTR_1g021945 | 57.328 | 232 | 87 | 6 | 25 | 247 | 20 | 248 | 2.68e-80 | 241 |
| MS.gene44238.t1 | MTR_1g062590 | 51.282 | 234 | 105 | 4 | 19 | 244 | 10 | 242 | 3.60e-79 | 238 |
| MS.gene44238.t1 | MTR_5g022350 | 68.790 | 157 | 49 | 0 | 1 | 157 | 66 | 222 | 2.38e-78 | 236 |
| MS.gene44238.t1 | MTR_1g062630 | 48.729 | 236 | 112 | 4 | 19 | 246 | 10 | 244 | 8.08e-77 | 233 |
| MS.gene44238.t1 | MTR_1g062370 | 50.847 | 236 | 107 | 4 | 19 | 246 | 10 | 244 | 1.77e-76 | 232 |
| MS.gene44238.t1 | MTR_2g069660 | 48.536 | 239 | 112 | 4 | 17 | 244 | 5 | 243 | 2.16e-76 | 231 |
| MS.gene44238.t1 | MTR_1g062350 | 50.847 | 236 | 107 | 4 | 19 | 246 | 10 | 244 | 2.21e-76 | 231 |
| MS.gene44238.t1 | MTR_1g062340 | 50.855 | 234 | 106 | 4 | 21 | 246 | 12 | 244 | 8.70e-76 | 230 |
| MS.gene44238.t1 | MTR_1g062660 | 49.383 | 243 | 114 | 4 | 12 | 246 | 3 | 244 | 9.39e-76 | 230 |
| MS.gene44238.t1 | MTR_5g059200 | 49.580 | 238 | 112 | 5 | 15 | 249 | 13 | 245 | 3.15e-75 | 230 |
| MS.gene44238.t1 | MTR_2g068030 | 48.182 | 220 | 104 | 5 | 33 | 243 | 25 | 243 | 2.15e-67 | 209 |
| MS.gene44238.t1 | MTR_3g068015 | 46.371 | 248 | 118 | 8 | 10 | 244 | 3 | 248 | 4.55e-67 | 208 |
| MS.gene44238.t1 | MTR_2g067980 | 46.939 | 245 | 117 | 6 | 15 | 247 | 7 | 250 | 1.99e-66 | 222 |
| MS.gene44238.t1 | MTR_2g068655 | 46.087 | 230 | 114 | 6 | 29 | 249 | 29 | 257 | 1.66e-64 | 203 |
| MS.gene44238.t1 | MTR_1g062690 | 45.532 | 235 | 97 | 4 | 19 | 246 | 10 | 220 | 6.48e-63 | 196 |
| MS.gene44238.t1 | MTR_7g062610 | 43.697 | 238 | 124 | 5 | 15 | 244 | 7 | 242 | 2.67e-62 | 196 |
| MS.gene44238.t1 | MTR_6g009480 | 44.309 | 246 | 120 | 9 | 15 | 245 | 10 | 253 | 1.08e-58 | 187 |
| MS.gene44238.t1 | MTR_4g063630 | 40.664 | 241 | 125 | 8 | 15 | 243 | 17 | 251 | 1.50e-54 | 176 |
| MS.gene44238.t1 | MTR_5g010640 | 41.350 | 237 | 120 | 8 | 16 | 244 | 11 | 236 | 3.80e-50 | 164 |
| MS.gene44238.t1 | MTR_5g010635 | 43.694 | 222 | 101 | 9 | 29 | 244 | 22 | 225 | 1.33e-48 | 160 |
| MS.gene44238.t1 | MTR_8g096900 | 40.773 | 233 | 119 | 9 | 14 | 244 | 33 | 248 | 1.52e-48 | 160 |
| MS.gene44238.t1 | MTR_5g023850 | 38.824 | 255 | 114 | 10 | 3 | 249 | 1 | 221 | 1.83e-45 | 152 |
| MS.gene44238.t1 | MTR_2g067990 | 41.176 | 221 | 108 | 8 | 28 | 246 | 24 | 224 | 8.34e-43 | 155 |
| MS.gene44238.t1 | MTR_8g089020 | 53.175 | 126 | 54 | 2 | 113 | 235 | 33 | 156 | 9.27e-42 | 140 |
| MS.gene44238.t1 | MTR_2g067970 | 40.642 | 187 | 101 | 6 | 69 | 247 | 1 | 185 | 7.04e-39 | 144 |
| MS.gene44238.t1 | MTR_7g076360 | 38.537 | 205 | 71 | 3 | 29 | 233 | 6 | 155 | 9.94e-39 | 133 |
| MS.gene44238.t1 | MTR_2g063150 | 33.193 | 238 | 136 | 8 | 14 | 235 | 12 | 242 | 1.17e-35 | 127 |
| MS.gene44238.t1 | MTR_1g062390 | 33.884 | 242 | 141 | 7 | 13 | 245 | 8 | 239 | 2.06e-35 | 126 |
| MS.gene44238.t1 | MTR_8g096910 | 42.581 | 155 | 77 | 8 | 15 | 167 | 6 | 150 | 1.99e-28 | 106 |
| MS.gene44238.t1 | MTR_8g096920 | 40.741 | 135 | 72 | 6 | 15 | 147 | 3 | 131 | 5.52e-25 | 97.4 |
| MS.gene44238.t1 | MTR_1g062640 | 38.739 | 111 | 64 | 3 | 58 | 167 | 39 | 146 | 1.01e-16 | 75.1 |
| MS.gene44238.t1 | MTR_5g068670 | 46.591 | 88 | 36 | 4 | 65 | 144 | 5 | 89 | 1.75e-13 | 65.9 |
| MS.gene44238.t1 | MTR_8g088960 | 43.750 | 80 | 41 | 3 | 122 | 199 | 8 | 85 | 1.19e-12 | 62.8 |
| MS.gene44238.t1 | MTR_3g081550 | 43.617 | 94 | 46 | 3 | 113 | 199 | 16 | 109 | 2.10e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44238.t1 | AT4G38660 | 64.659 | 249 | 82 | 3 | 1 | 246 | 2 | 247 | 5.06e-111 | 323 |
| MS.gene44238.t1 | AT4G38660 | 69.369 | 222 | 64 | 2 | 31 | 249 | 8 | 228 | 3.48e-109 | 318 |
| MS.gene44238.t1 | AT4G24180 | 62.069 | 261 | 85 | 6 | 1 | 248 | 1 | 260 | 1.96e-107 | 311 |
| MS.gene44238.t1 | AT4G24180 | 62.548 | 259 | 82 | 7 | 1 | 248 | 1 | 255 | 1.15e-106 | 309 |
| MS.gene44238.t1 | AT4G24180 | 62.403 | 258 | 83 | 6 | 1 | 245 | 1 | 257 | 1.69e-106 | 310 |
| MS.gene44238.t1 | AT4G24180 | 62.891 | 256 | 80 | 7 | 1 | 245 | 1 | 252 | 9.49e-106 | 308 |
| MS.gene44238.t1 | AT4G24180 | 60.166 | 241 | 83 | 5 | 1 | 228 | 1 | 241 | 1.82e-96 | 285 |
| MS.gene44238.t1 | AT4G24180 | 60.669 | 239 | 80 | 6 | 1 | 228 | 1 | 236 | 1.05e-95 | 283 |
| MS.gene44238.t1 | AT1G20030 | 60.870 | 230 | 78 | 4 | 28 | 245 | 17 | 246 | 1.43e-90 | 270 |
| MS.gene44238.t1 | AT1G20030 | 61.947 | 226 | 74 | 4 | 32 | 245 | 4 | 229 | 2.21e-90 | 269 |
| MS.gene44238.t1 | AT1G75800 | 57.202 | 243 | 92 | 4 | 15 | 245 | 7 | 249 | 2.38e-90 | 270 |
| MS.gene44238.t1 | AT1G75030 | 55.020 | 249 | 98 | 7 | 8 | 247 | 3 | 246 | 7.06e-86 | 256 |
| MS.gene44238.t1 | AT1G75050 | 56.017 | 241 | 94 | 7 | 14 | 245 | 7 | 244 | 1.05e-84 | 253 |
| MS.gene44238.t1 | AT2G17860 | 55.691 | 246 | 94 | 6 | 17 | 248 | 9 | 253 | 1.20e-84 | 253 |
| MS.gene44238.t1 | AT1G19320 | 53.755 | 253 | 100 | 7 | 1 | 243 | 1 | 246 | 1.06e-83 | 250 |
| MS.gene44238.t1 | AT4G36010 | 57.082 | 233 | 84 | 6 | 28 | 245 | 20 | 251 | 6.37e-81 | 245 |
| MS.gene44238.t1 | AT4G36010 | 57.082 | 233 | 84 | 6 | 28 | 245 | 20 | 251 | 6.37e-81 | 245 |
| MS.gene44238.t1 | AT1G75040 | 54.852 | 237 | 97 | 6 | 14 | 244 | 7 | 239 | 8.95e-81 | 243 |
| MS.gene44238.t1 | AT4G38670 | 52.439 | 246 | 103 | 7 | 12 | 244 | 3 | 247 | 2.20e-79 | 240 |
| MS.gene44238.t1 | AT4G38670 | 52.439 | 246 | 103 | 7 | 12 | 244 | 3 | 247 | 2.20e-79 | 240 |
| MS.gene44238.t1 | AT1G73620 | 54.825 | 228 | 92 | 5 | 29 | 248 | 40 | 264 | 2.81e-79 | 239 |
| MS.gene44238.t1 | AT1G18250 | 54.464 | 224 | 91 | 5 | 29 | 244 | 19 | 239 | 2.02e-78 | 236 |
| MS.gene44238.t1 | AT1G18250 | 54.018 | 224 | 92 | 5 | 29 | 244 | 20 | 240 | 3.09e-78 | 236 |
| MS.gene44238.t1 | AT1G77700 | 51.240 | 242 | 110 | 5 | 10 | 248 | 40 | 276 | 3.60e-78 | 238 |
| MS.gene44238.t1 | AT1G77700 | 51.883 | 239 | 107 | 5 | 10 | 245 | 40 | 273 | 4.49e-78 | 239 |
| MS.gene44238.t1 | AT5G24620 | 50.198 | 253 | 106 | 7 | 15 | 249 | 6 | 256 | 4.64e-77 | 239 |
| MS.gene44238.t1 | AT5G24620 | 50.198 | 253 | 106 | 7 | 15 | 249 | 61 | 311 | 7.38e-77 | 240 |
| MS.gene44238.t1 | AT5G24620 | 50.198 | 253 | 106 | 7 | 15 | 249 | 82 | 332 | 8.80e-77 | 241 |
| MS.gene44238.t1 | AT4G38670 | 53.509 | 228 | 95 | 5 | 28 | 244 | 4 | 231 | 1.06e-76 | 233 |
| MS.gene44238.t1 | AT1G77700 | 54.091 | 220 | 93 | 5 | 29 | 245 | 87 | 301 | 1.06e-75 | 233 |
| MS.gene44238.t1 | AT5G38280 | 46.502 | 243 | 118 | 4 | 15 | 245 | 9 | 251 | 8.60e-72 | 232 |
| MS.gene44238.t1 | AT5G38280 | 46.502 | 243 | 118 | 4 | 15 | 245 | 9 | 251 | 8.79e-72 | 232 |
| MS.gene44238.t1 | AT5G40020 | 43.651 | 252 | 131 | 5 | 4 | 246 | 1 | 250 | 1.75e-65 | 204 |
| MS.gene44238.t1 | AT4G36000 | 71.724 | 145 | 36 | 3 | 104 | 247 | 1 | 141 | 3.27e-64 | 199 |
| MS.gene44238.t1 | AT4G36000 | 71.724 | 145 | 36 | 3 | 104 | 247 | 1 | 141 | 3.27e-64 | 199 |
| MS.gene44238.t1 | AT5G02140 | 45.382 | 249 | 119 | 7 | 9 | 247 | 3 | 244 | 1.26e-63 | 201 |
| MS.gene44238.t1 | AT5G02140 | 45.528 | 246 | 117 | 7 | 9 | 244 | 3 | 241 | 1.94e-63 | 198 |
| MS.gene44238.t1 | AT4G18253 | 42.500 | 240 | 124 | 5 | 15 | 246 | 7 | 240 | 3.31e-59 | 187 |
| MS.gene44238.t1 | AT1G70250 | 43.860 | 228 | 113 | 5 | 32 | 247 | 146 | 370 | 2.50e-55 | 189 |
| MS.gene44238.t1 | AT2G28790 | 41.841 | 239 | 125 | 7 | 17 | 243 | 37 | 273 | 1.14e-53 | 174 |
| MS.gene44238.t1 | AT2G28790 | 41.841 | 239 | 125 | 7 | 17 | 243 | 12 | 248 | 1.15e-53 | 174 |
| MS.gene44238.t1 | AT4G18250 | 41.772 | 237 | 129 | 4 | 17 | 246 | 40 | 274 | 8.46e-51 | 176 |
| MS.gene44238.t1 | AT4G11650 | 44.017 | 234 | 112 | 11 | 15 | 246 | 9 | 225 | 4.59e-50 | 164 |
| MS.gene44238.t1 | AT5G24620 | 53.175 | 126 | 48 | 3 | 134 | 249 | 15 | 139 | 4.43e-37 | 132 |
| MS.gene44238.t1 | AT2G24810 | 58.974 | 78 | 28 | 2 | 169 | 243 | 116 | 192 | 7.39e-24 | 95.1 |
Find 75 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGCATAGCTATAAGATTT+TGG | 0.212867 | 8.1:-31656229 | None:intergenic |
| GGTTGCAAATTCGACGATTC+TGG | 0.256766 | 8.1:+31655830 | MS.gene44238:CDS |
| CCGGTTGGTCAGGTAGATTC+TGG | 0.283059 | 8.1:+31655798 | MS.gene44238:CDS |
| TCAACCGGAGACTGTGGTTC+CGG | 0.303738 | 8.1:+31655866 | MS.gene44238:CDS |
| ACAATATGGCCAGGAATTCT+AGG | 0.311650 | 8.1:+31655707 | MS.gene44238:CDS |
| TTGTTCGCCTTCGGTTTATT+CGG | 0.321676 | 8.1:+31656186 | MS.gene44238:CDS |
| CAACCGGAGACTGTGGTTCC+GGG | 0.352374 | 8.1:+31655867 | MS.gene44238:CDS |
| GGCCACGGAACCTGCTCAAC+CGG | 0.356065 | 8.1:+31655851 | MS.gene44238:CDS |
| GTTGAAACTAGTGGCGGTTC+AGG | 0.356585 | 8.1:+31656013 | MS.gene44238:CDS |
| ACAAATGTGATCACACAATA+TGG | 0.373841 | 8.1:+31655693 | MS.gene44238:CDS |
| AAACCGGTTGTTCCGAGGTC+TGG | 0.386392 | 8.1:-31655734 | None:intergenic |
| TATTGTTGTAGTGGCGCGTT+TGG | 0.396855 | 8.1:+31656151 | MS.gene44238:CDS |
| AATAGCGCGTGTGGTGCCTT+TGG | 0.406914 | 8.1:+31656118 | MS.gene44238:CDS |
| TGTAGACGGTTACAATCTTC+CGG | 0.423644 | 8.1:+31655985 | MS.gene44238:CDS |
| GAAATTAACTGCAACGGAAA+CGG | 0.426268 | 8.1:+31655890 | MS.gene44238:CDS |
| CAAGCACCAGCCGGTTGGTC+AGG | 0.426443 | 8.1:+31655788 | MS.gene44238:CDS |
| GTGAATTCTGCTAATGTTGC+CGG | 0.441903 | 8.1:-31655923 | None:intergenic |
| TATACATACCTTTGAAAGCT+AGG | 0.444402 | 8.1:-31655457 | None:intergenic |
| CGGAAACGGCGCAACACCAC+CGG | 0.452277 | 8.1:+31655904 | MS.gene44238:CDS |
| TTCCCCGGAACCACAGTCTC+CGG | 0.457171 | 8.1:-31655870 | None:intergenic |
| ATAGCGCGTGTGGTGCCTTT+GGG | 0.460528 | 8.1:+31656119 | MS.gene44238:CDS |
| GCCATCGAGTTGTTCGCCTT+CGG | 0.461378 | 8.1:+31656177 | MS.gene44238:CDS |
| AGACTAACATCGTAGTAATC+TGG | 0.463483 | 8.1:-31655962 | None:intergenic |
| TCATGTCAGCCCACTGGATG+TGG | 0.463727 | 8.1:+31656037 | MS.gene44238:CDS |
| AAACCAGACCTCGGAACAAC+CGG | 0.464546 | 8.1:+31655731 | MS.gene44238:CDS |
| TCCGTTGCAGTTAATTTCCC+CGG | 0.478576 | 8.1:-31655885 | None:intergenic |
| CTTTGAAAGCTAGGATAAGT+AGG | 0.489482 | 8.1:-31655448 | None:intergenic |
| CGGTTACAATCTTCCGGTGA+TGG | 0.491856 | 8.1:+31655991 | MS.gene44238:CDS |
| CTGACCAACCGGCTGGTGCT+TGG | 0.493351 | 8.1:-31655787 | None:intergenic |
| CGGTTGGTCAGGTAGATTCT+GGG | 0.493549 | 8.1:+31655799 | MS.gene44238:CDS |
| CTACTTATCCTAGCTTTCAA+AGG | 0.495706 | 8.1:+31655449 | MS.gene44238:CDS |
| GGTAGATTCTGGGCAAGAAC+CGG | 0.501018 | 8.1:+31655809 | MS.gene44238:CDS |
| ACCACAGTCTCCGGTTGAGC+AGG | 0.501097 | 8.1:-31655861 | None:intergenic |
| CCAACCGGCTGGTGCTTGGA+AGG | 0.506602 | 8.1:-31655783 | None:intergenic |
| TAAGTAGGAAAATGAAGAAA+CGG | 0.509403 | 8.1:-31655433 | None:intergenic |
| GATGTGGAGAGGATCTTAAC+CGG | 0.515133 | 8.1:+31656053 | MS.gene44238:CDS |
| ATTTACCTTCGAGAAGAAGA+AGG | 0.516114 | 8.1:-31656313 | None:intergenic |
| AGAATCGTCGAATTTGCAAC+CGG | 0.526091 | 8.1:-31655828 | None:intergenic |
| CCGTCCACTCTCAGCTCCGA+TGG | 0.526529 | 8.1:-31656082 | None:intergenic |
| CCAGAATCTACCTGACCAAC+CGG | 0.527173 | 8.1:-31655798 | None:intergenic |
| TCAGGTTCATGTCAGCCCAC+TGG | 0.530267 | 8.1:+31656031 | MS.gene44238:CDS |
| TGTGATCACACAATATGGCC+AGG | 0.532969 | 8.1:+31655698 | MS.gene44238:CDS |
| GTGTGGTGCCTTTGGGACAC+CGG | 0.551624 | 8.1:+31656126 | MS.gene44238:CDS |
| AAATTCGACGATTCTGGCCA+CGG | 0.552629 | 8.1:+31655836 | MS.gene44238:CDS |
| TTTGTCCTTCTTCTTCTCGA+AGG | 0.553524 | 8.1:+31656308 | MS.gene44238:CDS |
| AACATTTCCGAATAAACCGA+AGG | 0.568030 | 8.1:-31656193 | None:intergenic |
| CCATCGGAGCTGAGAGTGGA+CGG | 0.581824 | 8.1:+31656082 | MS.gene44238:CDS |
| GTGTCCATCGGAGCTGAGAG+TGG | 0.585045 | 8.1:+31656078 | MS.gene44238:CDS |
| CTCCGGTTGAGCAGGTTCCG+TGG | 0.586582 | 8.1:-31655853 | None:intergenic |
| ACACCGGAGTATTGTTGTAG+TGG | 0.587807 | 8.1:+31656142 | MS.gene44238:CDS |
| CCACTAGTTTCAACCATCAC+CGG | 0.588717 | 8.1:-31656004 | None:intergenic |
| TCCGGGGAAATTAACTGCAA+CGG | 0.590336 | 8.1:+31655884 | MS.gene44238:CDS |
| AATCTACCTGACCAACCGGC+TGG | 0.593577 | 8.1:-31655794 | None:intergenic |
| TACGATGTTAGTCTTGTAGA+CGG | 0.595413 | 8.1:+31655971 | MS.gene44238:CDS |
| ACATTAGCAGAATTCACTCT+AGG | 0.595724 | 8.1:+31655929 | MS.gene44238:CDS |
| ACCGAAGGCGAACAACTCGA+TGG | 0.601385 | 8.1:-31656178 | None:intergenic |
| AACCGGAGACTGTGGTTCCG+GGG | 0.604183 | 8.1:+31655868 | MS.gene44238:CDS |
| AAGATCCTCTCCACATCCAG+TGG | 0.606360 | 8.1:-31656047 | None:intergenic |
| TCAGCTCCGATGGACACCTC+CGG | 0.607057 | 8.1:-31656072 | None:intergenic |
| ACCTGCTCAACCGGAGACTG+TGG | 0.608729 | 8.1:+31655860 | MS.gene44238:CDS |
| ACAATACTCCGGTGTCCCAA+AGG | 0.614212 | 8.1:-31656134 | None:intergenic |
| TCTTAACCGGAGGTGTCCAT+CGG | 0.615290 | 8.1:+31656066 | MS.gene44238:CDS |
| CCTTCCAAGCACCAGCCGGT+TGG | 0.621791 | 8.1:+31655783 | MS.gene44238:CDS |
| GTTCAAAACCGGTTGTTCCG+AGG | 0.625163 | 8.1:-31655739 | None:intergenic |
| CAAACCTTCCAAGCACCAGC+CGG | 0.627518 | 8.1:+31655779 | MS.gene44238:CDS |
| CCGGTGATGGTTGAAACTAG+TGG | 0.635688 | 8.1:+31656004 | MS.gene44238:CDS |
| AGATCCTCTCCACATCCAGT+GGG | 0.647063 | 8.1:-31656046 | None:intergenic |
| GACGCGTGTAATAGCGCGTG+TGG | 0.652762 | 8.1:+31656109 | MS.gene44238:CDS |
| TCAGCCCACTGGATGTGGAG+AGG | 0.658980 | 8.1:+31656042 | MS.gene44238:CDS |
| ATTCTAGGAAAACCAGACCT+CGG | 0.680245 | 8.1:+31655722 | MS.gene44238:CDS |
| AATTCTGCTAATGTTGCCGG+TGG | 0.701173 | 8.1:-31655920 | None:intergenic |
| GCGCCACTACAACAATACTC+CGG | 0.703825 | 8.1:-31656145 | None:intergenic |
| GTGGAGAGGATCTTAACCGG+AGG | 0.704810 | 8.1:+31656056 | MS.gene44238:CDS |
| TCGGAGCTGAGAGTGGACGG+TGG | 0.723814 | 8.1:+31656085 | MS.gene44238:CDS |
| GTGATGGTTGAAACTAGTGG+CGG | 0.740161 | 8.1:+31656007 | MS.gene44238:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTCATATATGTATTGA+TGG | - | chr8.1:31655587-31655606 | None:intergenic | 15.0% |
| ! | TAAGTAGGAAAATGAAGAAA+CGG | - | chr8.1:31655436-31655455 | None:intergenic | 25.0% |
| !! | ATATAACGACGATTTTTGAT+TGG | - | chr8.1:31655549-31655568 | None:intergenic | 25.0% |
| ACAAATGTGATCACACAATA+TGG | + | chr8.1:31655693-31655712 | MS.gene44238:CDS | 30.0% | |
| ! | TACGCATAGCTATAAGATTT+TGG | - | chr8.1:31656232-31656251 | None:intergenic | 30.0% |
| ! | TATACATACCTTTGAAAGCT+AGG | - | chr8.1:31655460-31655479 | None:intergenic | 30.0% |
| !!! | TCCTTTTTTGAGTTCAAAAC+CGG | - | chr8.1:31655753-31655772 | None:intergenic | 30.0% |
| AACATTTCCGAATAAACCGA+AGG | - | chr8.1:31656196-31656215 | None:intergenic | 35.0% | |
| AATTGTTACTCCTGCATGTA+TGG | - | chr8.1:31655648-31655667 | None:intergenic | 35.0% | |
| ACAATTATTCCCATACATGC+AGG | + | chr8.1:31655635-31655654 | MS.gene44238:intron | 35.0% | |
| ACATTAGCAGAATTCACTCT+AGG | + | chr8.1:31655929-31655948 | MS.gene44238:CDS | 35.0% | |
| AGACTAACATCGTAGTAATC+TGG | - | chr8.1:31655965-31655984 | None:intergenic | 35.0% | |
| ATTGTTACTCCTGCATGTAT+GGG | - | chr8.1:31655647-31655666 | None:intergenic | 35.0% | |
| GAAATTAACTGCAACGGAAA+CGG | + | chr8.1:31655890-31655909 | MS.gene44238:CDS | 35.0% | |
| ! | CTACTTATCCTAGCTTTCAA+AGG | + | chr8.1:31655449-31655468 | MS.gene44238:CDS | 35.0% |
| ! | CTTTGAAAGCTAGGATAAGT+AGG | - | chr8.1:31655451-31655470 | None:intergenic | 35.0% |
| ! | GCTATAAGATTTTGGACAAG+CGG | - | chr8.1:31656224-31656243 | None:intergenic | 35.0% |
| ! | TACTTTTACTTGTACTGCTG+CGG | + | chr8.1:31656267-31656286 | MS.gene44238:CDS | 35.0% |
| !! | TACGATGTTAGTCTTGTAGA+CGG | + | chr8.1:31655971-31655990 | MS.gene44238:CDS | 35.0% |
| !!! | ACCGGTTTTGAACTCAAAAA+AGG | + | chr8.1:31655749-31655768 | MS.gene44238:CDS | 35.0% |
| ACAATATGGCCAGGAATTCT+AGG | + | chr8.1:31655707-31655726 | MS.gene44238:CDS | 40.0% | |
| AGAATCGTCGAATTTGCAAC+CGG | - | chr8.1:31655831-31655850 | None:intergenic | 40.0% | |
| GTGAATTCTGCTAATGTTGC+CGG | - | chr8.1:31655926-31655945 | None:intergenic | 40.0% | |
| TGTAGACGGTTACAATCTTC+CGG | + | chr8.1:31655985-31656004 | MS.gene44238:CDS | 40.0% | |
| TTGTTCGCCTTCGGTTTATT+CGG | + | chr8.1:31656186-31656205 | MS.gene44238:CDS | 40.0% | |
| TTTGTCCTTCTTCTTCTCGA+AGG | + | chr8.1:31656308-31656327 | MS.gene44238:CDS | 40.0% | |
| ! | ATTCTAGGAAAACCAGACCT+CGG | + | chr8.1:31655722-31655741 | MS.gene44238:CDS | 40.0% |
| !!! | TCTGGTTTTCCTAGAATTCC+TGG | - | chr8.1:31655719-31655738 | None:intergenic | 40.0% |
| AAATTCGACGATTCTGGCCA+CGG | + | chr8.1:31655836-31655855 | MS.gene44238:CDS | 45.0% | |
| AATTCTGCTAATGTTGCCGG+TGG | - | chr8.1:31655923-31655942 | None:intergenic | 45.0% | |
| GATGTGGAGAGGATCTTAAC+CGG | + | chr8.1:31656053-31656072 | MS.gene44238:CDS | 45.0% | |
| GGTTGCAAATTCGACGATTC+TGG | + | chr8.1:31655830-31655849 | MS.gene44238:CDS | 45.0% | |
| GTGATGGTTGAAACTAGTGG+CGG | + | chr8.1:31656007-31656026 | MS.gene44238:CDS | 45.0% | |
| TATTGTTGTAGTGGCGCGTT+TGG | + | chr8.1:31656151-31656170 | MS.gene44238:CDS | 45.0% | |
| TCCGGGGAAATTAACTGCAA+CGG | + | chr8.1:31655884-31655903 | MS.gene44238:CDS | 45.0% | |
| TCCGTTGCAGTTAATTTCCC+CGG | - | chr8.1:31655888-31655907 | None:intergenic | 45.0% | |
| TGTGATCACACAATATGGCC+AGG | + | chr8.1:31655698-31655717 | MS.gene44238:CDS | 45.0% | |
| ! | CCACTAGTTTCAACCATCAC+CGG | - | chr8.1:31656007-31656026 | None:intergenic | 45.0% |
| !! | ACACCGGAGTATTGTTGTAG+TGG | + | chr8.1:31656142-31656161 | MS.gene44238:CDS | 45.0% |
| AAACCAGACCTCGGAACAAC+CGG | + | chr8.1:31655731-31655750 | MS.gene44238:CDS | 50.0% | |
| AAGATCCTCTCCACATCCAG+TGG | - | chr8.1:31656050-31656069 | None:intergenic | 50.0% | |
| ACAATACTCCGGTGTCCCAA+AGG | - | chr8.1:31656137-31656156 | None:intergenic | 50.0% | |
| AGATCCTCTCCACATCCAGT+GGG | - | chr8.1:31656049-31656068 | None:intergenic | 50.0% | |
| CCAGAATCTACCTGACCAAC+CGG | - | chr8.1:31655801-31655820 | None:intergenic | 50.0% | |
| CGGTTACAATCTTCCGGTGA+TGG | + | chr8.1:31655991-31656010 | MS.gene44238:CDS | 50.0% | |
| GCGCCACTACAACAATACTC+CGG | - | chr8.1:31656148-31656167 | None:intergenic | 50.0% | |
| GGTAGATTCTGGGCAAGAAC+CGG | + | chr8.1:31655809-31655828 | MS.gene44238:CDS | 50.0% | |
| GTTCAAAACCGGTTGTTCCG+AGG | - | chr8.1:31655742-31655761 | None:intergenic | 50.0% | |
| GTTGAAACTAGTGGCGGTTC+AGG | + | chr8.1:31656013-31656032 | MS.gene44238:CDS | 50.0% | |
| ! | CCGGTGATGGTTGAAACTAG+TGG | + | chr8.1:31656004-31656023 | MS.gene44238:CDS | 50.0% |
| ! | CGGTTGGTCAGGTAGATTCT+GGG | + | chr8.1:31655799-31655818 | MS.gene44238:CDS | 50.0% |
| !! | TCTTAACCGGAGGTGTCCAT+CGG | + | chr8.1:31656066-31656085 | MS.gene44238:CDS | 50.0% |
| AAACCGGTTGTTCCGAGGTC+TGG | - | chr8.1:31655737-31655756 | None:intergenic | 55.0% | |
| AATAGCGCGTGTGGTGCCTT+TGG | + | chr8.1:31656118-31656137 | MS.gene44238:CDS | 55.0% | |
| AATCTACCTGACCAACCGGC+TGG | - | chr8.1:31655797-31655816 | None:intergenic | 55.0% | |
| ACCGAAGGCGAACAACTCGA+TGG | - | chr8.1:31656181-31656200 | None:intergenic | 55.0% | |
| ATAGCGCGTGTGGTGCCTTT+GGG | + | chr8.1:31656119-31656138 | MS.gene44238:CDS | 55.0% | |
| CAAACCTTCCAAGCACCAGC+CGG | + | chr8.1:31655779-31655798 | MS.gene44238:CDS | 55.0% | |
| GTGGAGAGGATCTTAACCGG+AGG | + | chr8.1:31656056-31656075 | MS.gene44238:CDS | 55.0% | |
| TCAACCGGAGACTGTGGTTC+CGG | + | chr8.1:31655866-31655885 | MS.gene44238:CDS | 55.0% | |
| TCAGGTTCATGTCAGCCCAC+TGG | + | chr8.1:31656031-31656050 | MS.gene44238:CDS | 55.0% | |
| TCATGTCAGCCCACTGGATG+TGG | + | chr8.1:31656037-31656056 | MS.gene44238:CDS | 55.0% | |
| ! | CCGGTTGGTCAGGTAGATTC+TGG | + | chr8.1:31655798-31655817 | MS.gene44238:CDS | 55.0% |
| ! | GCCATCGAGTTGTTCGCCTT+CGG | + | chr8.1:31656177-31656196 | MS.gene44238:CDS | 55.0% |
| AACCGGAGACTGTGGTTCCG+GGG | + | chr8.1:31655868-31655887 | MS.gene44238:CDS | 60.0% | |
| ACCACAGTCTCCGGTTGAGC+AGG | - | chr8.1:31655864-31655883 | None:intergenic | 60.0% | |
| ACCTGCTCAACCGGAGACTG+TGG | + | chr8.1:31655860-31655879 | MS.gene44238:CDS | 60.0% | |
| CAACCGGAGACTGTGGTTCC+GGG | + | chr8.1:31655867-31655886 | MS.gene44238:CDS | 60.0% | |
| CCATCGGAGCTGAGAGTGGA+CGG | + | chr8.1:31656082-31656101 | MS.gene44238:CDS | 60.0% | |
| GACGCGTGTAATAGCGCGTG+TGG | + | chr8.1:31656109-31656128 | MS.gene44238:CDS | 60.0% | |
| GTGTCCATCGGAGCTGAGAG+TGG | + | chr8.1:31656078-31656097 | MS.gene44238:CDS | 60.0% | |
| TCAGCCCACTGGATGTGGAG+AGG | + | chr8.1:31656042-31656061 | MS.gene44238:CDS | 60.0% | |
| TCAGCTCCGATGGACACCTC+CGG | - | chr8.1:31656075-31656094 | None:intergenic | 60.0% | |
| TTCCCCGGAACCACAGTCTC+CGG | - | chr8.1:31655873-31655892 | None:intergenic | 60.0% | |
| !! | GTGTGGTGCCTTTGGGACAC+CGG | + | chr8.1:31656126-31656145 | MS.gene44238:CDS | 60.0% |
| CCAACCGGCTGGTGCTTGGA+AGG | - | chr8.1:31655786-31655805 | None:intergenic | 65.0% | |
| CCGTCCACTCTCAGCTCCGA+TGG | - | chr8.1:31656085-31656104 | None:intergenic | 65.0% | |
| CCTTCCAAGCACCAGCCGGT+TGG | + | chr8.1:31655783-31655802 | MS.gene44238:CDS | 65.0% | |
| CGGAAACGGCGCAACACCAC+CGG | + | chr8.1:31655904-31655923 | MS.gene44238:CDS | 65.0% | |
| CTCCGGTTGAGCAGGTTCCG+TGG | - | chr8.1:31655856-31655875 | None:intergenic | 65.0% | |
| CTGACCAACCGGCTGGTGCT+TGG | - | chr8.1:31655790-31655809 | None:intergenic | 65.0% | |
| GGCCACGGAACCTGCTCAAC+CGG | + | chr8.1:31655851-31655870 | MS.gene44238:CDS | 65.0% | |
| TCGGAGCTGAGAGTGGACGG+TGG | + | chr8.1:31656085-31656104 | MS.gene44238:CDS | 65.0% | |
| ! | CAAGCACCAGCCGGTTGGTC+AGG | + | chr8.1:31655788-31655807 | MS.gene44238:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 31655398 | 31656333 | 31655398 | ID=MS.gene44238 |
| chr8.1 | mRNA | 31655398 | 31656333 | 31655398 | ID=MS.gene44238.t1;Parent=MS.gene44238 |
| chr8.1 | exon | 31655398 | 31655470 | 31655398 | ID=MS.gene44238.t1.exon1;Parent=MS.gene44238.t1 |
| chr8.1 | CDS | 31655398 | 31655470 | 31655398 | ID=cds.MS.gene44238.t1;Parent=MS.gene44238.t1 |
| chr8.1 | exon | 31655657 | 31656333 | 31655657 | ID=MS.gene44238.t1.exon2;Parent=MS.gene44238.t1 |
| chr8.1 | CDS | 31655657 | 31656333 | 31655657 | ID=cds.MS.gene44238.t1;Parent=MS.gene44238.t1 |
| Gene Sequence |
| Protein sequence |
