Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47280.t1 | XP_013451520.1 | 97 | 1013 | 26 | 2 | 1 | 1013 | 1 | 1009 | 0.00E+00 | 1734.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47280.t1 | B8XCH5 | 50.3 | 1081 | 444 | 16 | 10 | 1013 | 17 | 1081 | 1.0e-238 | 828.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47280.t1 | A0A072U8X0 | 97.0 | 1013 | 26 | 2 | 1 | 1013 | 1 | 1009 | 0.0e+00 | 1734.2 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049596 | MS.gene47280 | 0.814529 | 1.47E-51 | -1.69E-46 |
| MS.gene049989 | MS.gene47280 | 0.853194 | 2.81E-61 | -1.69E-46 |
| MS.gene050297 | MS.gene47280 | 0.883016 | 6.41E-71 | -1.69E-46 |
| MS.gene050310 | MS.gene47280 | 0.808306 | 3.30E-50 | -1.69E-46 |
| MS.gene050648 | MS.gene47280 | 0.832674 | 8.26E-56 | -1.69E-46 |
| MS.gene051263 | MS.gene47280 | 0.849902 | 2.40E-60 | -1.69E-46 |
| MS.gene051478 | MS.gene47280 | 0.818578 | 1.82E-52 | -1.69E-46 |
| MS.gene051629 | MS.gene47280 | 0.809405 | 1.92E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene47280 | 0.83237 | 9.83E-56 | -1.69E-46 |
| MS.gene052143 | MS.gene47280 | 0.804288 | 2.32E-49 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47280.t1 | MTR_6g027540 | 97.236 | 1013 | 24 | 2 | 1 | 1013 | 1 | 1009 | 0.0 | 2017 |
| MS.gene47280.t1 | MTR_4g107850 | 45.920 | 1054 | 492 | 22 | 8 | 1013 | 15 | 1038 | 0.0 | 899 |
| MS.gene47280.t1 | MTR_5g010390 | 43.690 | 1046 | 503 | 22 | 11 | 1013 | 5 | 1007 | 0.0 | 853 |
| MS.gene47280.t1 | MTR_6g028050 | 42.938 | 1055 | 526 | 23 | 6 | 1013 | 1 | 1026 | 0.0 | 831 |
| MS.gene47280.t1 | MTR_8g031270 | 41.905 | 1050 | 512 | 20 | 11 | 1013 | 5 | 1003 | 0.0 | 822 |
| MS.gene47280.t1 | MTR_7g092770 | 51.232 | 771 | 324 | 13 | 272 | 1013 | 28 | 775 | 0.0 | 784 |
| MS.gene47280.t1 | MTR_0305s0020 | 51.432 | 768 | 325 | 12 | 272 | 1013 | 29 | 774 | 0.0 | 782 |
| MS.gene47280.t1 | MTR_7g076900 | 41.801 | 1055 | 524 | 23 | 7 | 1013 | 1 | 1013 | 0.0 | 771 |
| MS.gene47280.t1 | MTR_0291s0010 | 48.642 | 773 | 345 | 14 | 272 | 1013 | 46 | 797 | 0.0 | 747 |
| MS.gene47280.t1 | MTR_4g066150 | 49.741 | 772 | 342 | 15 | 264 | 1013 | 23 | 770 | 0.0 | 745 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47280.t1 | AT3G03680 | 69.485 | 1029 | 273 | 15 | 9 | 1013 | 6 | 1017 | 0.0 | 1401 |
| MS.gene47280.t1 | AT4G11610 | 43.624 | 1043 | 516 | 19 | 6 | 1013 | 2 | 1007 | 0.0 | 867 |
| MS.gene47280.t1 | AT4G11610 | 43.457 | 1047 | 516 | 19 | 6 | 1013 | 2 | 1011 | 0.0 | 866 |
| MS.gene47280.t1 | AT5G17980 | 42.740 | 1095 | 494 | 32 | 6 | 1013 | 1 | 1049 | 0.0 | 834 |
| MS.gene47280.t1 | AT5G48060 | 43.097 | 1072 | 500 | 23 | 11 | 1013 | 6 | 1036 | 0.0 | 833 |
| MS.gene47280.t1 | AT1G04150 | 44.004 | 1034 | 515 | 21 | 11 | 1013 | 12 | 1012 | 0.0 | 825 |
| MS.gene47280.t1 | AT1G22610 | 41.082 | 1054 | 547 | 18 | 9 | 1013 | 1 | 1029 | 0.0 | 795 |
| MS.gene47280.t1 | AT3G57880 | 50.911 | 768 | 328 | 13 | 272 | 1013 | 29 | 773 | 0.0 | 777 |
| MS.gene47280.t1 | AT3G57880 | 50.911 | 768 | 328 | 13 | 272 | 1013 | 29 | 773 | 0.0 | 777 |
| MS.gene47280.t1 | AT3G57880 | 50.911 | 768 | 328 | 13 | 272 | 1013 | 29 | 773 | 0.0 | 777 |
Find 229 sgRNAs with CRISPR-Local
Find 276 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAAAGACGGGACACG+TGG | 0.639862 | 6.4:+2347698 | MS.gene47280:CDS |
| AAAAGAAGAAAATACAAAAG+AGG | 0.474664 | 6.4:+2345995 | MS.gene47280:CDS |
| AAAAGCACAAACCCTTTAAA+CGG | 0.491505 | 6.4:-2348748 | None:intergenic |
| AAATCCACCGGCGAATCCAC+CGG | 0.518908 | 6.4:+2346067 | MS.gene47280:CDS |
| AACAACTGATGCTTATGTGG+TGG | 0.739510 | 6.4:+2347721 | MS.gene47280:CDS |
| AACAATGAGGTCTGTGGAAG+TGG | 0.457588 | 6.4:+2348552 | MS.gene47280:CDS |
| AACAGAGATTTCACTCGGAA+CGG | 0.593560 | 6.4:+2346523 | MS.gene47280:CDS |
| AACTCCAGCAGAGAAAACTC+CGG | 0.662938 | 6.4:+2346037 | MS.gene47280:CDS |
| AACTGCACCGGCGCCGGAGG+TGG | 0.406115 | 6.4:+2346091 | MS.gene47280:CDS |
| AACTGCTATGAAGATAGTAA+CGG | 0.650640 | 6.4:+2348141 | MS.gene47280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTGCTATGAAGATAGTAA+CGG | + | chr6.4:2348141-2348160 | MS.gene47280:CDS | 30.0% | |
| AACTTGATGAAGAGTTTGAT+GGG | + | chr6.4:2348524-2348543 | MS.gene47280:CDS | 30.0% | |
| AAGAATCTAATGCCAAAAGA+CGG | + | chr6.4:2345492-2345511 | MS.gene47280:CDS | 30.0% | |
| AAGAGATTTGAATCCTCAAT+GGG | + | chr6.4:2345584-2345603 | MS.gene47280:CDS | 30.0% | |
| AATAAGAATTCACGTAGACA+CGG | - | chr6.4:2347949-2347968 | None:intergenic | 30.0% | |
| AGAAGAAAATACAAAAGAGG+AGG | + | chr6.4:2345998-2346017 | MS.gene47280:CDS | 30.0% | |
| ATAATCTGATCAGAAAACGA+AGG | - | chr6.4:2348853-2348872 | None:intergenic | 30.0% | |
| CAAATAACAGAGATTTCACT+CGG | + | chr6.4:2346518-2346537 | MS.gene47280:CDS | 30.0% | |
| GGTTTAACATAATGCATTCT+TGG | - | chr6.4:2348088-2348107 | None:intergenic | 30.0% | |
| GTTTCGAATTCAAGATCAAT+TGG | + | chr6.4:2347004-2347023 | MS.gene47280:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 2345435 | 2348876 | 2345435 | ID=MS.gene47280 |
| chr6.4 | mRNA | 2345435 | 2348876 | 2345435 | ID=MS.gene47280.t1;Parent=MS.gene47280 |
| chr6.4 | exon | 2345435 | 2347136 | 2345435 | ID=MS.gene47280.t1.exon1;Parent=MS.gene47280.t1 |
| chr6.4 | CDS | 2345435 | 2347136 | 2345435 | ID=cds.MS.gene47280.t1;Parent=MS.gene47280.t1 |
| chr6.4 | exon | 2347537 | 2348876 | 2347537 | ID=MS.gene47280.t1.exon2;Parent=MS.gene47280.t1 |
| chr6.4 | CDS | 2347537 | 2348876 | 2347537 | ID=cds.MS.gene47280.t1;Parent=MS.gene47280.t1 |
| Gene Sequence |
>MS.gene47280 ATGACTACGACGGAAGTAGTTTCTGTAAGGAAACTAATCGTCGAAGTCATCAATGCAAAGAATCTAATGCCAAAAGACGGTCAAGGAACAGCGAGCGCTTACGCGATCGTTGATTTCGACGGTCAAAGAAAGCGAACAAAGACAAAATCAAGAGATTTGAATCCTCAATGGGACGAGAAACTCGAGTTTCTTGTCCTTGACCAAGAGTCAATGACCTCTGAGACGATGGAGATCAATCTTTACAATGACAAAAAAACAACTGGGAAGAGAAGTACTTTTTTAGGAAAAGTTAAAATCTCTGGAAGCACGTTTGTGAAAAGCGGTGAAGAAGTGATTGTTTACTATCCGTTAGAGAAAAGGAGCGTTTTCTCTCAGATTAAAGGTGAACTTGGTTTGAAAATTTCTTACGTGGATGAAATTTTACCTGTTACCGATTCAACCGGTGATGAAAAGAAAGATGAGAAAGTTGAAGAGAAGTCACCGGTAACCGAATCAGCCGGTGATAGTGAGAAAAAAGACGAGAATCCACCGGTTACCGATTCAGCCGGTGATGAGAAGAAAAAAGAAGAAAATACAAAAGAGGAGGAAAAACCAAAAGAAGAAACTCCAGCAGAGAAAACTCCGGCACCGGAAAATCCACCGGCGAATCCACCGGCAACTGCACCGGCGCCGGAGGTGGTGAATCCACCGGTAGCTGAAACGAAGGATGTGAAAATCAAAGCAAAAGAACAATATGAAATTGTTCAGAAACGTGGTGATGTGATCAATGTGAGTGATCACGAGCTTCGATCTCTGAGTAGAGATCGAAGCAGAAGTGCGGCATACGATCTAGTTGATCGTATGCCATTTCTATATGTTCGCGTTGTTAAAGCGAAGCGATGTGAATCAAAAGCTGAATCTGTGAAGCTTTTCTCAAAGCTTGTGATTGGAACTCATAGTGTGAGAACAAAGAGTGAAAGTGAAGGTAAAGATTGGGATCAGGTTTTCGCTTTTGATAAAGAAGGACTTAATTCAACTTCTTTAGAAGTTTCCGTTTGGTTAGAATCAGAATCAGAGTCATCAGAATCAGAAAACAAAGAGAAGCAAATAACAGAGATTTCACTCGGAACGGTGTCGTTTGATTTGCAAGAAGTTCCGAAGAGAGTTCCACCGGATAGTCCATTAGCACCGCAATGGTATACGCTTGAATCGGAAAATTCACCGGGGAATGACGTTATGATTGCAGTATGGATTGGGACGCAAGCCGATGAAGCTTTTCAGGAGTCGTGGCAGTCGGATTCCGGTGGTTTGATACCGGAGACACGAGCGAAAGTTTATCTTTCTCCGAAGCTGTGGTATCTGAGATTAACGGTTATCCAAACCCAGGATTTACAGCTGGGTTTGGGTGGTTTGGGTTCCGAACCTAAGGTTCGGAGTCCTGAGCTTTATGTTAAGGCTCAGCTTGGTGCACAGGTTTTTAAAACCGGTCGGATTGGTTTAGTCAACTCGGGTAATCCGACGTGGAATGAAGATCTGGTTTTTGTAGCTTCTGAGCCGTTTGAACCGTTTCTAGTCATTACATTAGAAGATGTTTCGAATTCAAGATCAATTGGGAAAACGAAGATCCATGTGGCTTCGATCGAGAGGAGATTAGATGATCGGACGGATGTGAAGTCGAGATGGTTTAATCTTTGTGGTTCAGAGGAAAATCTTTCTTACACAGGTAAATGTTGCTCTTTGTCCAAATATATTGCATATTTTACGGTTGATAGTGTAGCTAATGTGTTCATTTTATATATGTATATAGTTGATTTTGATTTTTGATATTGCAACGTACTTAGTGATATTAATTAAGAGTTTTCCTTCAAAAAATACTATCAATTAGGAGTTTATTTAGAAAAGAATCAACAAATGTATCTAGTAATTTGAAAATGAGCTTATATTTAGAGACGGAGAAAATAGTAAATTATTGTGTTATGTTAGTTTATAATTTAGAACTCATTTAGCGTCTCTTATGTCATGAACTTAGTTATCTGCATGAGATGTTCAATGATTATGTCATTAATTTAATTGTATATGATCCTGCTCCCACTAATTGACATTTTGGATTTGTCTCTGCACAGGTAGGATACATATTCGTGCTTGTTTAGAAGGTGGATATCATGTGATAGATGAAGCAGCTCATGTGACTAGCGATGTCCGTGCTTCTGCGAAGCAGTTAACGAAGCCTCCAATTGGTTTACTTGAAGTTGGGATTCGTGGAGCAACGAATCTTCTTCCGGTGAAAACCAAAGACGGGACACGTGGAACAACTGATGCTTATGTGGTGGCGAAATATGGTCCAAAATGGGTTCGAACTAGGACTATTGTAGACCGGTTTAACCCGAGATGGAATGAGCAATACACATGGGATGTTTATGATCCTTGCACGGTTCTCACAATTGGTGTTTTCGATAATGGAAGGTTTCAGAAAGATGCTAGAGATGTTAGAATGGGGAAAATTCGTGTGCGGTTGTCTACACTAGACACTAACCGTGTCTACGTGAATTCTTATTCGTTGATTGTTTTACTGCCAGGTGGTGCGAGGAGAATGGGTGAGATTGAAATCGCAGTTAGATTTTCATGTTCTTCATGGTTGAGTTTAATGCAGGCTTATACAAGTCCAATTCTACCAAGAATGCATTATGTTAAACCATTCGGTCCGGGTCAACAAGACGTTTTGAGACAAACTGCTATGAAGATAGTAACGGCTCGTTTAGCTCGGTCTGAACCGGCTTTGGGTTCAGAAGTGGTTCAGTTCATGCTTGATTCGGATACTCATGTGTGGAGCATGAGGAAAAGTAAAGCGAATTGGTTTAGATTAGTTGGATTTTTGTCACGTTCGACGACTGTGTTTTATTGGTTAGATGGAATTCGCACGTGGGTGAATCCGGCAACAACGGTTTTAGTTCACGCGCTTCTCATAGCGATTGTGTTCTGTCCTTATTTGATTCTACCAACAGTTTTTATGTATGCTTTCTTGATTCTGATTTTGAGGTTTAGGAACAGAATGAGGGTGCCAAAGAACATGGATCCGAGGATGTCCTACGTGGACATGGTAAGTTTGGATGAACTTGATGAAGAGTTTGATGGGTTTCCAACAATGAGGTCTGTGGAAGTGGTTCGAATCAGGTACGATAGGGTGCGTGCTCTTGCGGGGAGGGCACAAAGTTTGATTGGCGATGTTGCAGCTCAGGGCGAGAGATTGGAAGCTTTGTTTAGTTGGAGAGACCCACGTGCTACAGCGATGTTCGCTGTGTTCTGTTTGGTAATGTCGTTGGTTTTCTATGCTGTGCCGTTTAAAGGGTTTGTGCTTTTGGCTGGGTTTTATTATCTGAGGCATCCGAGGTTTCGCGGTGACATGCCATCTGTTTTCGTGAACTTCTTTCGGAGACTTCCTTCGTTTTCTGATCAGATTATGTAA |
| Protein sequence |
>MS.gene47280.t1 MTTTEVVSVRKLIVEVINAKNLMPKDGQGTASAYAIVDFDGQRKRTKTKSRDLNPQWDEKLEFLVLDQESMTSETMEINLYNDKKTTGKRSTFLGKVKISGSTFVKSGEEVIVYYPLEKRSVFSQIKGELGLKISYVDEILPVTDSTGDEKKDEKVEEKSPVTESAGDSEKKDENPPVTDSAGDEKKKEENTKEEEKPKEETPAEKTPAPENPPANPPATAPAPEVVNPPVAETKDVKIKAKEQYEIVQKRGDVINVSDHELRSLSRDRSRSAAYDLVDRMPFLYVRVVKAKRCESKAESVKLFSKLVIGTHSVRTKSESEGKDWDQVFAFDKEGLNSTSLEVSVWLESESESSESENKEKQITEISLGTVSFDLQEVPKRVPPDSPLAPQWYTLESENSPGNDVMIAVWIGTQADEAFQESWQSDSGGLIPETRAKVYLSPKLWYLRLTVIQTQDLQLGLGGLGSEPKVRSPELYVKAQLGAQVFKTGRIGLVNSGNPTWNEDLVFVASEPFEPFLVITLEDVSNSRSIGKTKIHVASIERRLDDRTDVKSRWFNLCGSEENLSYTGRIHIRACLEGGYHVIDEAAHVTSDVRASAKQLTKPPIGLLEVGIRGATNLLPVKTKDGTRGTTDAYVVAKYGPKWVRTRTIVDRFNPRWNEQYTWDVYDPCTVLTIGVFDNGRFQKDARDVRMGKIRVRLSTLDTNRVYVNSYSLIVLLPGGARRMGEIEIAVRFSCSSWLSLMQAYTSPILPRMHYVKPFGPGQQDVLRQTAMKIVTARLARSEPALGSEVVQFMLDSDTHVWSMRKSKANWFRLVGFLSRSTTVFYWLDGIRTWVNPATTVLVHALLIAIVFCPYLILPTVFMYAFLILILRFRNRMRVPKNMDPRMSYVDMVSLDELDEEFDGFPTMRSVEVVRIRYDRVRALAGRAQSLIGDVAAQGERLEALFSWRDPRATAMFAVFCLVMSLVFYAVPFKGFVLLAGFYYLRHPRFRGDMPSVFVNFFRRLPSFSDQIM |
