Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051698.t1 | XP_013448602.1 | 97.8 | 602 | 13 | 0 | 1 | 602 | 1 | 602 | 1.40E-287 | 998.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051698.t1 | Q55GA4 | 38.6 | 603 | 329 | 9 | 35 | 598 | 33 | 633 | 1.7e-55 | 218.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051698.t1 | A0A072TYP8 | 97.8 | 602 | 13 | 0 | 1 | 602 | 1 | 602 | 1.0e-287 | 998.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049989 | MS.gene051698 | 0.8138 | 2.13E-51 | -1.69E-46 |
| MS.gene050297 | MS.gene051698 | 0.835902 | 1.28E-56 | -1.69E-46 |
| MS.gene051263 | MS.gene051698 | 0.808121 | 3.62E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene052367 | 0.836747 | 7.80E-57 | -1.69E-46 |
| MS.gene051698 | MS.gene053416 | 0.822826 | 1.92E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene053417 | 0.823683 | 1.21E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene054643 | 0.844211 | 8.70E-59 | -1.69E-46 |
| MS.gene051698 | MS.gene061260 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene061805 | 0.814236 | 1.71E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene062855 | 0.805049 | 1.61E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene066031 | 0.819114 | 1.38E-52 | -1.69E-46 |
| MS.gene051698 | MS.gene066551 | 0.801981 | 6.97E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene066552 | 0.818662 | 1.74E-52 | -1.69E-46 |
| MS.gene051698 | MS.gene066877 | 0.820734 | 5.87E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene067039 | 0.806938 | 6.45E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene07028 | 0.817514 | 3.17E-52 | -1.69E-46 |
| MS.gene051698 | MS.gene073862 | 0.815654 | 8.28E-52 | -1.69E-46 |
| MS.gene051698 | MS.gene073863 | 0.811498 | 6.79E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene21320 | 0.805466 | 1.32E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene23980 | 0.800473 | 1.42E-48 | -1.69E-46 |
| MS.gene051698 | MS.gene24699 | 0.842236 | 2.93E-58 | -1.69E-46 |
| MS.gene051698 | MS.gene26224 | 0.837015 | 6.67E-57 | -1.69E-46 |
| MS.gene051698 | MS.gene26801 | 0.801704 | 7.94E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene28050 | 0.810191 | 1.30E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene28787 | 0.816408 | 5.62E-52 | -1.69E-46 |
| MS.gene051698 | MS.gene30201 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene051698 | MS.gene32703 | 0.803403 | 3.55E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene36292 | 0.830204 | 3.35E-55 | -1.69E-46 |
| MS.gene051698 | MS.gene38714 | 0.837045 | 6.55E-57 | -1.69E-46 |
| MS.gene051698 | MS.gene42665 | 0.803925 | 2.76E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene47280 | 0.83237 | 9.83E-56 | -1.69E-46 |
| MS.gene051698 | MS.gene52975 | 0.822653 | 2.11E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene57320 | 0.820245 | 7.59E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene57701 | 0.801291 | 9.65E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene58096 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene59466 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene66156 | 0.807388 | 5.18E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene66158 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene66159 | 0.841546 | 4.45E-58 | -1.69E-46 |
| MS.gene051698 | MS.gene67576 | 0.811953 | 5.41E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene70896 | 0.820097 | 8.21E-53 | -1.69E-46 |
| MS.gene051698 | MS.gene70898 | 0.836427 | 9.42E-57 | -1.69E-46 |
| MS.gene051698 | MS.gene71419 | 0.811402 | 7.13E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene74328 | 0.811935 | 5.46E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene77475 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene78156 | 0.813273 | 2.78E-51 | -1.69E-46 |
| MS.gene051698 | MS.gene78972 | 0.800364 | 1.49E-48 | -1.69E-46 |
| MS.gene051698 | MS.gene80180 | 0.80644 | 8.22E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene80183 | 0.804731 | 1.88E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene84014 | 0.802117 | 6.53E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene89565 | 0.801256 | 9.81E-49 | -1.69E-46 |
| MS.gene051698 | MS.gene99093 | 0.811737 | 6.03E-51 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051698.t1 | MTR_7g056153 | 96.860 | 605 | 13 | 3 | 1 | 602 | 1 | 602 | 0.0 | 1201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051698.t1 | AT3G25100 | 67.381 | 607 | 182 | 3 | 1 | 602 | 1 | 596 | 0.0 | 849 |
Find 133 sgRNAs with CRISPR-Local
Find 160 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTAATAGTTCTGGGAATT+TGG | 0.185937 | 7.3:+49157062 | MS.gene051698:CDS |
| TCCGCTGCTGATGTTGTTTA+TGG | 0.207546 | 7.3:+49157453 | MS.gene051698:CDS |
| ATGAGTTGTTTGAGTCTTCT+TGG | 0.235274 | 7.3:+49157949 | MS.gene051698:CDS |
| GGAGGTGTGGTTAGTGTTAT+GGG | 0.266116 | 7.3:-49156446 | None:intergenic |
| TGCTTCCACTACTATTGATT+TGG | 0.270053 | 7.3:+49156684 | MS.gene051698:CDS |
| CTGCTCAAGGAAATGCGTTC+GGG | 0.276711 | 7.3:+49157880 | MS.gene051698:CDS |
| GGATTCATGGTTATAGTTCT+AGG | 0.283095 | 7.3:+49157427 | MS.gene051698:CDS |
| AAGAAGAAGAAAGAGTATTA+TGG | 0.288347 | 7.3:+49156862 | MS.gene051698:CDS |
| GAGCAGTATATTAATAGTTC+TGG | 0.290818 | 7.3:+49157054 | MS.gene051698:CDS |
| TAAAGCAGTTAATTCTTTCA+TGG | 0.305065 | 7.3:+49157986 | MS.gene051698:CDS |
| TTATGGCGTGACTGCGCTTT+TGG | 0.310974 | 7.3:+49157470 | MS.gene051698:CDS |
| CGAATAAAGTTCTTGTTGTT+GGG | 0.318755 | 7.3:+49157832 | MS.gene051698:CDS |
| TGGGATGCAAAAGGCTATTA+AGG | 0.319371 | 7.3:+49157587 | MS.gene051698:CDS |
| TTCTTGTTGTTGGGGTTTGT+GGG | 0.321732 | 7.3:+49157841 | MS.gene051698:CDS |
| TTTGGAGTCGTTCGTGAATT+CGG | 0.323881 | 7.3:+49157488 | MS.gene051698:CDS |
| TGAAAGATGGGACGAAGATT+AGG | 0.328092 | 7.3:+49157106 | MS.gene051698:CDS |
| TATGGATTGGGCACATTTCA+TGG | 0.332860 | 7.3:+49156880 | MS.gene051698:CDS |
| TGGAGGTGTGGTTAGTGTTA+TGG | 0.340343 | 7.3:-49156447 | None:intergenic |
| CCAACAACAAGAACTTTATT+CGG | 0.343201 | 7.3:-49157831 | None:intergenic |
| TCCATCTCCATCTGCAGAAA+TGG | 0.344329 | 7.3:-49156791 | None:intergenic |
| CCGAATAAAGTTCTTGTTGT+TGG | 0.348360 | 7.3:+49157831 | MS.gene051698:CDS |
| TCTTCGCAAAATTCTTAACT+TGG | 0.349466 | 7.3:+49156501 | MS.gene051698:CDS |
| GCTGCTCAAGGAAATGCGTT+CGG | 0.353508 | 7.3:+49157879 | MS.gene051698:CDS |
| GTTCTTGTTGTTGGGGTTTG+TGG | 0.354406 | 7.3:+49157840 | MS.gene051698:CDS |
| ACATTTCATGGGAAGCCTTC+AGG | 0.354708 | 7.3:+49156892 | MS.gene051698:CDS |
| GAAAGATGGGACGAAGATTA+GGG | 0.361537 | 7.3:+49157107 | MS.gene051698:CDS |
| GGAATCGACGTCGGAGGTTG+AGG | 0.365604 | 7.3:-49156314 | None:intergenic |
| GAACAACAACAGAATCATTC+TGG | 0.367864 | 7.3:-49156588 | None:intergenic |
| GGTTTGTGGGAGGCCTCGTT+TGG | 0.391938 | 7.3:+49157854 | MS.gene051698:CDS |
| TAAGCAATCCTGGACGAATT+CGG | 0.403035 | 7.3:-49157132 | None:intergenic |
| GAAGAAAGAGTATTATGGAT+TGG | 0.404272 | 7.3:+49156867 | MS.gene051698:CDS |
| AATGGATGCTTTGAGAGAAA+AGG | 0.409633 | 7.3:+49157764 | MS.gene051698:CDS |
| GCCGAAGCTGATGCTGTTGC+AGG | 0.409863 | 7.3:+49157164 | MS.gene051698:CDS |
| GGGATGTGGTGGAAGAAAGT+TGG | 0.410002 | 7.3:-49156276 | None:intergenic |
| AATTCTGTTACTTTGAAAGA+TGG | 0.420708 | 7.3:+49157093 | MS.gene051698:CDS |
| GATCTGTGCTTGTATGTCTC+AGG | 0.429059 | 7.3:+49157806 | MS.gene051698:CDS |
| AAGAAAGAGTATTATGGATT+GGG | 0.430822 | 7.3:+49156868 | MS.gene051698:CDS |
| GAAGAAGTTGAAACTGTTGT+TGG | 0.433738 | 7.3:+49157263 | MS.gene051698:CDS |
| CATTTCATGGGAAGCCTTCA+GGG | 0.439608 | 7.3:+49156893 | MS.gene051698:CDS |
| TCTGCGGATTCTAAACTGTT+GGG | 0.441601 | 7.3:+49157705 | MS.gene051698:CDS |
| CTCATCCTCATAAGCAATCC+TGG | 0.442048 | 7.3:-49157142 | None:intergenic |
| AGAATACCGATGAATTGCTT+TGG | 0.442265 | 7.3:+49156950 | MS.gene051698:CDS |
| AAATCAGTAAGTCCATATTC+CGG | 0.451792 | 7.3:-49157384 | None:intergenic |
| AATGTTGATAAGCTCAAAGA+TGG | 0.453499 | 7.3:+49157567 | MS.gene051698:CDS |
| ATAACGAAGATAGTAGCATT+AGG | 0.456575 | 7.3:-49156526 | None:intergenic |
| CGGATTCGAGAATACGGAAA+AGG | 0.457489 | 7.3:-49156354 | None:intergenic |
| TTCGTCCAGGATTGCTTATG+AGG | 0.458166 | 7.3:+49157137 | MS.gene051698:CDS |
| AAGATCCGGTGAAAATGTAT+AGG | 0.461080 | 7.3:+49156839 | MS.gene051698:CDS |
| AGCAGTATATTAATAGTTCT+GGG | 0.462246 | 7.3:+49157055 | MS.gene051698:CDS |
| GATTCATGGTTATAGTTCTA+GGG | 0.467181 | 7.3:+49157428 | MS.gene051698:CDS |
| AAACTGTTGTTGGCTAGGAT+GGG | 0.473055 | 7.3:+49157273 | MS.gene051698:CDS |
| GAGGTATAGTGATGGTGTTA+TGG | 0.474525 | 7.3:+49157026 | MS.gene051698:CDS |
| AGAAATTCCATTTCTGCAGA+TGG | 0.479320 | 7.3:+49156784 | MS.gene051698:CDS |
| GGATGTGGTGGAAGAAAGTT+GGG | 0.479824 | 7.3:-49156275 | None:intergenic |
| AAGAAAGTTGGGCGGATTGA+CGG | 0.483248 | 7.3:-49156264 | None:intergenic |
| GAAACTGTTGTTGGCTAGGA+TGG | 0.484688 | 7.3:+49157272 | MS.gene051698:CDS |
| CTGAGATTATGAAGATGAAT+CGG | 0.486327 | 7.3:-49156562 | None:intergenic |
| CCTCGTTTGGCTGCTGCTCA+AGG | 0.493809 | 7.3:+49157867 | MS.gene051698:CDS |
| CTTCGCAAAATTCTTAACTT+GGG | 0.495268 | 7.3:+49156502 | MS.gene051698:CDS |
| TGCGTTTAGAAGTGCGGCTG+AGG | 0.495604 | 7.3:+49157905 | MS.gene051698:CDS |
| TTGAATCGGATTCGAGAATA+CGG | 0.496269 | 7.3:-49156360 | None:intergenic |
| TTGAAGACGTGGAGTGATAA+CGG | 0.498431 | 7.3:+49157237 | MS.gene051698:CDS |
| GCATTAGCAAGAGCAGTAAG+AGG | 0.498639 | 7.3:-49156664 | None:intergenic |
| TCCATTTCTGCAGATGGAGA+TGG | 0.500250 | 7.3:+49156790 | MS.gene051698:CDS |
| ATTTCGCGGAAGGATGAAAC+GGG | 0.500501 | 7.3:-49156400 | None:intergenic |
| AGATGAAGAGCGTCAAGATC+CGG | 0.502389 | 7.3:+49156825 | MS.gene051698:CDS |
| ATTCTGTTACTTTGAAAGAT+GGG | 0.502719 | 7.3:+49157094 | MS.gene051698:CDS |
| TTGAGGGGAAAATGAGGACG+GGG | 0.504650 | 7.3:-49156297 | None:intergenic |
| GGATTCGAGAATACGGAAAA+GGG | 0.506807 | 7.3:-49156353 | None:intergenic |
| ATGGATGCTTTGAGAGAAAA+GGG | 0.510225 | 7.3:+49157765 | MS.gene051698:CDS |
| CAAAACTTTGTCAAAGCTTG+AGG | 0.512346 | 7.3:-49157732 | None:intergenic |
| CTCTGACTCAGACTCCGAAT+CGG | 0.513676 | 7.3:+49156720 | MS.gene051698:CDS |
| TCCTGCAACAGCATCAGCTT+CGG | 0.515180 | 7.3:-49157165 | None:intergenic |
| GTCTGCGGATTCTAAACTGT+TGG | 0.516003 | 7.3:+49157704 | MS.gene051698:CDS |
| AGATGCGTATTGAATTGAAT+CGG | 0.517102 | 7.3:-49156374 | None:intergenic |
| GAATAAAGTTCTTGTTGTTG+GGG | 0.518055 | 7.3:+49157833 | MS.gene051698:CDS |
| GAATCGACGTCGGAGGTTGA+GGG | 0.518794 | 7.3:-49156313 | None:intergenic |
| GTTGAGGGGAAAATGAGGAC+GGG | 0.522776 | 7.3:-49156298 | None:intergenic |
| TTCAGAATCTGAATCCGATT+CGG | 0.522991 | 7.3:-49156734 | None:intergenic |
| GAGTCGTTCGTGAATTCGGA+TGG | 0.524667 | 7.3:+49157492 | MS.gene051698:CDS |
| GCTCAAAGATGGGATGCAAA+AGG | 0.532197 | 7.3:+49157578 | MS.gene051698:CDS |
| AGGTGTGGTTAGTGTTATGG+GGG | 0.534117 | 7.3:-49156444 | None:intergenic |
| AGTTGAAACTGTTGTTGGCT+AGG | 0.535306 | 7.3:+49157268 | MS.gene051698:CDS |
| AAGAAGACTCAAACAACTCA+TGG | 0.542997 | 7.3:-49157947 | None:intergenic |
| GAGGTGTGGTTAGTGTTATG+GGG | 0.544233 | 7.3:-49156445 | None:intergenic |
| TTGTTGTTGGGGTTTGTGGG+AGG | 0.545204 | 7.3:+49157844 | MS.gene051698:CDS |
| TTAGGGCACCGAATTCGTCC+AGG | 0.545508 | 7.3:+49157124 | MS.gene051698:CDS |
| AGCTGATGCTGTTGCAGGAG+TGG | 0.553964 | 7.3:+49157169 | MS.gene051698:CDS |
| CCGATCAATTTGTGCACGAG+AGG | 0.554137 | 7.3:+49156992 | MS.gene051698:CDS |
| ATGGATTGGGCACATTTCAT+GGG | 0.554848 | 7.3:+49156881 | MS.gene051698:CDS |
| AATTTCGCGGAAGGATGAAA+CGG | 0.554887 | 7.3:-49156401 | None:intergenic |
| TAGTTCTGGGAATTTGGATG+CGG | 0.555180 | 7.3:+49157068 | MS.gene051698:CDS |
| AACTGTTGTTGGCTAGGATG+GGG | 0.557759 | 7.3:+49157274 | MS.gene051698:CDS |
| GGTGTGGTTAGTGTTATGGG+GGG | 0.559356 | 7.3:-49156443 | None:intergenic |
| TAGTGATGGTGTTATGGAGT+TGG | 0.564329 | 7.3:+49157032 | MS.gene051698:CDS |
| AGCTAACCAAAGCAATTCAT+CGG | 0.567480 | 7.3:-49156956 | None:intergenic |
| TGAGGGGAAAATGAGGACGG+GGG | 0.569417 | 7.3:-49156296 | None:intergenic |
| CGGGGTTGCGTTTAGAAGTG+CGG | 0.569454 | 7.3:+49157899 | MS.gene051698:CDS |
| CGGAGGTTGAGGGGAAAATG+AGG | 0.576744 | 7.3:-49156303 | None:intergenic |
| ATTGCTTTGGTTAGCTTGTG+TGG | 0.578683 | 7.3:+49156963 | MS.gene051698:CDS |
| AATCGACGTCGGAGGTTGAG+GGG | 0.581195 | 7.3:-49156312 | None:intergenic |
| TAGCATAATTATGAATTTCG+CGG | 0.581607 | 7.3:-49156414 | None:intergenic |
| GACATACAAGCACAGATCAA+AGG | 0.583268 | 7.3:-49157801 | None:intergenic |
| GAAGTGGAAGGAAGTTTCGA+TGG | 0.584074 | 7.3:+49157667 | MS.gene051698:CDS |
| AGACTCCAAATCAATAGTAG+TGG | 0.587865 | 7.3:-49156689 | None:intergenic |
| GCCCCAGTTGATTAAAACAA+TGG | 0.591920 | 7.3:-49156467 | None:intergenic |
| ACGGATATATTCGAAGTGGA+AGG | 0.597096 | 7.3:+49157655 | MS.gene051698:CDS |
| TCTGCAGATGGAGATGGAGA+TGG | 0.600422 | 7.3:+49156796 | MS.gene051698:CDS |
| TGCTCAAGGAAATGCGTTCG+GGG | 0.600482 | 7.3:+49157881 | MS.gene051698:CDS |
| CTGCGGATTCTAAACTGTTG+GGG | 0.601525 | 7.3:+49157706 | MS.gene051698:CDS |
| AAAGGCTATTAAGGTTCAGA+GGG | 0.602140 | 7.3:+49157596 | MS.gene051698:CDS |
| CCTCTCGTGCACAAATTGAT+CGG | 0.607763 | 7.3:-49156992 | None:intergenic |
| TGCTTCGATGCAGTTTAATG+TGG | 0.608675 | 7.3:+49157521 | MS.gene051698:CDS |
| GTTTCAGTATATGAATGTTG+AGG | 0.608759 | 7.3:+49157323 | MS.gene051698:CDS |
| AAAAGGCTATTAAGGTTCAG+AGG | 0.612575 | 7.3:+49157595 | MS.gene051698:CDS |
| ATAATTATGAATTTCGCGGA+AGG | 0.617801 | 7.3:-49156410 | None:intergenic |
| TGATTAAAACAATGGAGGTG+TGG | 0.618405 | 7.3:-49156459 | None:intergenic |
| GTTGAAGCTTGACGAGTCTG+CGG | 0.622496 | 7.3:+49157689 | MS.gene051698:CDS |
| CCTTGAGCAGCAGCCAAACG+AGG | 0.631943 | 7.3:-49157867 | None:intergenic |
| TCATACATCAAACACCCTGA+AGG | 0.632800 | 7.3:-49156907 | None:intergenic |
| GGTTGAGGGGAAAATGAGGA+CGG | 0.641159 | 7.3:-49156299 | None:intergenic |
| ATGTTGATAAGCTCAAAGAT+GGG | 0.642662 | 7.3:+49157568 | MS.gene051698:CDS |
| GATTATGAAGATGAATCGGA+CGG | 0.649594 | 7.3:-49156558 | None:intergenic |
| CCAGTTGATTAAAACAATGG+AGG | 0.650743 | 7.3:-49156464 | None:intergenic |
| CTTTGAGAGAAAAGGGTGCG+AGG | 0.654784 | 7.3:+49157772 | MS.gene051698:CDS |
| TGCGCAGAGGGAATCGACGT+CGG | 0.658090 | 7.3:-49156323 | None:intergenic |
| GCCATAAACAACATCAGCAG+CGG | 0.659326 | 7.3:-49157454 | None:intergenic |
| TTGCAACTAAATTGAAGACG+TGG | 0.664821 | 7.3:+49157226 | MS.gene051698:CDS |
| AGTGATGAGAGGTATAGTGA+TGG | 0.665061 | 7.3:+49157018 | MS.gene051698:CDS |
| GCAGAGGGAATCGACGTCGG+AGG | 0.677702 | 7.3:-49156320 | None:intergenic |
| ACGAGAGGTTGAGTGATGAG+AGG | 0.679264 | 7.3:+49157007 | MS.gene051698:CDS |
| TGTGGTGGAAGAAAGTTGGG+CGG | 0.702673 | 7.3:-49156272 | None:intergenic |
| TTTCGCGGAAGGATGAAACG+GGG | 0.801246 | 7.3:-49156399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTGTTATTTTCTAA+TGG | + | chr7.3:49157746-49157765 | MS.gene051698:CDS | 15.0% |
| !!! | TCTCTTTCTTGATTTTTTAT+CGG | - | chr7.3:49156761-49156780 | None:intergenic | 20.0% |
| !!! | TTACTGATTTTTATTATCGT+GGG | + | chr7.3:49157397-49157416 | MS.gene051698:CDS | 20.0% |
| ! | AGCAGTATATTAATAGTTCT+GGG | + | chr7.3:49157055-49157074 | MS.gene051698:CDS | 25.0% |
| ! | TAAAGCAGTTAATTCTTTCA+TGG | + | chr7.3:49157986-49158005 | MS.gene051698:CDS | 25.0% |
| ! | TAGCATAATTATGAATTTCG+CGG | - | chr7.3:49156417-49156436 | None:intergenic | 25.0% |
| !!! | AAGAAAGAGTATTATGGATT+GGG | + | chr7.3:49156868-49156887 | MS.gene051698:CDS | 25.0% |
| !!! | AAGAAGAAGAAAGAGTATTA+TGG | + | chr7.3:49156862-49156881 | MS.gene051698:CDS | 25.0% |
| !!! | AATTCTGTTACTTTGAAAGA+TGG | + | chr7.3:49157093-49157112 | MS.gene051698:CDS | 25.0% |
| !!! | ATTCTGTTACTTTGAAAGAT+GGG | + | chr7.3:49157094-49157113 | MS.gene051698:CDS | 25.0% |
| !!! | CTTACTGATTTTTATTATCG+TGG | + | chr7.3:49157396-49157415 | MS.gene051698:CDS | 25.0% |
| !!! | TATTAATAGTTCTGGGAATT+TGG | + | chr7.3:49157062-49157081 | MS.gene051698:CDS | 25.0% |
| AAAAACGGATATATTCGAAG+TGG | + | chr7.3:49157651-49157670 | MS.gene051698:CDS | 30.0% | |
| AAATCAGTAAGTCCATATTC+CGG | - | chr7.3:49157387-49157406 | None:intergenic | 30.0% | |
| AGATGCGTATTGAATTGAAT+CGG | - | chr7.3:49156377-49156396 | None:intergenic | 30.0% | |
| AGTCTTGCGATTATGAAAAA+CGG | + | chr7.3:49157636-49157655 | MS.gene051698:CDS | 30.0% | |
| ATAACGAAGATAGTAGCATT+AGG | - | chr7.3:49156529-49156548 | None:intergenic | 30.0% | |
| ATAATTATGAATTTCGCGGA+AGG | - | chr7.3:49156413-49156432 | None:intergenic | 30.0% | |
| CCAACAACAAGAACTTTATT+CGG | - | chr7.3:49157834-49157853 | None:intergenic | 30.0% | |
| CTGAGATTATGAAGATGAAT+CGG | - | chr7.3:49156565-49156584 | None:intergenic | 30.0% | |
| CTTCGCAAAATTCTTAACTT+GGG | + | chr7.3:49156502-49156521 | MS.gene051698:CDS | 30.0% | |
| GAGCAGTATATTAATAGTTC+TGG | + | chr7.3:49157054-49157073 | MS.gene051698:CDS | 30.0% | |
| GTTTCAGTATATGAATGTTG+AGG | + | chr7.3:49157323-49157342 | MS.gene051698:CDS | 30.0% | |
| TCTTCGCAAAATTCTTAACT+TGG | + | chr7.3:49156501-49156520 | MS.gene051698:CDS | 30.0% | |
| ! | AATGTTGATAAGCTCAAAGA+TGG | + | chr7.3:49157567-49157586 | MS.gene051698:CDS | 30.0% |
| ! | ATGTTGATAAGCTCAAAGAT+GGG | + | chr7.3:49157568-49157587 | MS.gene051698:CDS | 30.0% |
| ! | GATTCATGGTTATAGTTCTA+GGG | + | chr7.3:49157428-49157447 | MS.gene051698:CDS | 30.0% |
| ! | TTCTTCCTATACATTTTCAC+CGG | - | chr7.3:49156847-49156866 | None:intergenic | 30.0% |
| ! | TTTATTATCGTGGGTTTTTG+AGG | + | chr7.3:49157406-49157425 | MS.gene051698:CDS | 30.0% |
| !! | CGAATAAAGTTCTTGTTGTT+GGG | + | chr7.3:49157832-49157851 | MS.gene051698:CDS | 30.0% |
| !! | GAAGAAAGAGTATTATGGAT+TGG | + | chr7.3:49156867-49156886 | MS.gene051698:CDS | 30.0% |
| !! | GAATAAAGTTCTTGTTGTTG+GGG | + | chr7.3:49157833-49157852 | MS.gene051698:CDS | 30.0% |
| !! | TGTTGTTGTTCTTTTTACTC+AGG | + | chr7.3:49156600-49156619 | MS.gene051698:CDS | 30.0% |
| !!! | CTCCATTGTTTTAATCAACT+GGG | + | chr7.3:49156465-49156484 | MS.gene051698:CDS | 30.0% |
| !!! | TCCATTGTTTTAATCAACTG+GGG | + | chr7.3:49156466-49156485 | MS.gene051698:CDS | 30.0% |
| AAAAGGCTATTAAGGTTCAG+AGG | + | chr7.3:49157595-49157614 | MS.gene051698:CDS | 35.0% | |
| AAAGGCTATTAAGGTTCAGA+GGG | + | chr7.3:49157596-49157615 | MS.gene051698:CDS | 35.0% | |
| AAGAAGACTCAAACAACTCA+TGG | - | chr7.3:49157950-49157969 | None:intergenic | 35.0% | |
| AAGATCCGGTGAAAATGTAT+AGG | + | chr7.3:49156839-49156858 | MS.gene051698:CDS | 35.0% | |
| AGAAATTCCATTTCTGCAGA+TGG | + | chr7.3:49156784-49156803 | MS.gene051698:CDS | 35.0% | |
| AGAATACCGATGAATTGCTT+TGG | + | chr7.3:49156950-49156969 | MS.gene051698:CDS | 35.0% | |
| AGACTCCAAATCAATAGTAG+TGG | - | chr7.3:49156692-49156711 | None:intergenic | 35.0% | |
| AGCTAACCAAAGCAATTCAT+CGG | - | chr7.3:49156959-49156978 | None:intergenic | 35.0% | |
| ATGAGTTGTTTGAGTCTTCT+TGG | + | chr7.3:49157949-49157968 | MS.gene051698:CDS | 35.0% | |
| CAAAACTTTGTCAAAGCTTG+AGG | - | chr7.3:49157735-49157754 | None:intergenic | 35.0% | |
| CCAGTTGATTAAAACAATGG+AGG | - | chr7.3:49156467-49156486 | None:intergenic | 35.0% | |
| GAACAACAACAGAATCATTC+TGG | - | chr7.3:49156591-49156610 | None:intergenic | 35.0% | |
| GAAGAAGTTGAAACTGTTGT+TGG | + | chr7.3:49157263-49157282 | MS.gene051698:CDS | 35.0% | |
| GAGGTGAAAAAGAAGATGAA+GGG | + | chr7.3:49157342-49157361 | MS.gene051698:CDS | 35.0% | |
| GATTATGAAGATGAATCGGA+CGG | - | chr7.3:49156561-49156580 | None:intergenic | 35.0% | |
| GGATTCATGGTTATAGTTCT+AGG | + | chr7.3:49157427-49157446 | MS.gene051698:CDS | 35.0% | |
| TGAGGTGAAAAAGAAGATGA+AGG | + | chr7.3:49157341-49157360 | MS.gene051698:CDS | 35.0% | |
| TGATTAAAACAATGGAGGTG+TGG | - | chr7.3:49156462-49156481 | None:intergenic | 35.0% | |
| TTCAGAATCTGAATCCGATT+CGG | - | chr7.3:49156737-49156756 | None:intergenic | 35.0% | |
| TTGAATCGGATTCGAGAATA+CGG | - | chr7.3:49156363-49156382 | None:intergenic | 35.0% | |
| TTGCAACTAAATTGAAGACG+TGG | + | chr7.3:49157226-49157245 | MS.gene051698:CDS | 35.0% | |
| ! | CCGAATAAAGTTCTTGTTGT+TGG | + | chr7.3:49157831-49157850 | MS.gene051698:CDS | 35.0% |
| ! | TGAGTTTGAGAAGTTTTTGC+CGG | + | chr7.3:49157365-49157384 | MS.gene051698:CDS | 35.0% |
| ! | TGCTTCCACTACTATTGATT+TGG | + | chr7.3:49156684-49156703 | MS.gene051698:CDS | 35.0% |
| !! | AATGGATGCTTTGAGAGAAA+AGG | + | chr7.3:49157764-49157783 | MS.gene051698:CDS | 35.0% |
| !! | ATGGATGCTTTGAGAGAAAA+GGG | + | chr7.3:49157765-49157784 | MS.gene051698:CDS | 35.0% |
| !!! | CCTCCATTGTTTTAATCAAC+TGG | + | chr7.3:49156464-49156483 | MS.gene051698:CDS | 35.0% |
| AATTTCGCGGAAGGATGAAA+CGG | - | chr7.3:49156404-49156423 | None:intergenic | 40.0% | |
| ACGGATATATTCGAAGTGGA+AGG | + | chr7.3:49157655-49157674 | MS.gene051698:CDS | 40.0% | |
| GAAAGATGGGACGAAGATTA+GGG | + | chr7.3:49157107-49157126 | MS.gene051698:CDS | 40.0% | |
| GACATACAAGCACAGATCAA+AGG | - | chr7.3:49157804-49157823 | None:intergenic | 40.0% | |
| GCCCCAGTTGATTAAAACAA+TGG | - | chr7.3:49156470-49156489 | None:intergenic | 40.0% | |
| GGATTCGAGAATACGGAAAA+GGG | - | chr7.3:49156356-49156375 | None:intergenic | 40.0% | |
| TAAGCAATCCTGGACGAATT+CGG | - | chr7.3:49157135-49157154 | None:intergenic | 40.0% | |
| TCATACATCAAACACCCTGA+AGG | - | chr7.3:49156910-49156929 | None:intergenic | 40.0% | |
| TCTGCGGATTCTAAACTGTT+GGG | + | chr7.3:49157705-49157724 | MS.gene051698:CDS | 40.0% | |
| TGAAAGATGGGACGAAGATT+AGG | + | chr7.3:49157106-49157125 | MS.gene051698:CDS | 40.0% | |
| TGCTTCGATGCAGTTTAATG+TGG | + | chr7.3:49157521-49157540 | MS.gene051698:CDS | 40.0% | |
| TGGGATGCAAAAGGCTATTA+AGG | + | chr7.3:49157587-49157606 | MS.gene051698:CDS | 40.0% | |
| TTGAAGACGTGGAGTGATAA+CGG | + | chr7.3:49157237-49157256 | MS.gene051698:CDS | 40.0% | |
| ! | AAACTGTTGTTGGCTAGGAT+GGG | + | chr7.3:49157273-49157292 | MS.gene051698:CDS | 40.0% |
| ! | AGTGATGAGAGGTATAGTGA+TGG | + | chr7.3:49157018-49157037 | MS.gene051698:CDS | 40.0% |
| ! | AGTTGAAACTGTTGTTGGCT+AGG | + | chr7.3:49157268-49157287 | MS.gene051698:CDS | 40.0% |
| ! | ATGAGCTAGCTCATTTTCTG+AGG | + | chr7.3:49156926-49156945 | MS.gene051698:CDS | 40.0% |
| ! | GAGAAGTTTTTGCCGGAATA+TGG | + | chr7.3:49157372-49157391 | MS.gene051698:CDS | 40.0% |
| ! | GAGGTATAGTGATGGTGTTA+TGG | + | chr7.3:49157026-49157045 | MS.gene051698:CDS | 40.0% |
| ! | TTTGGAGTCGTTCGTGAATT+CGG | + | chr7.3:49157488-49157507 | MS.gene051698:CDS | 40.0% |
| !! | ATGGATTGGGCACATTTCAT+GGG | + | chr7.3:49156881-49156900 | MS.gene051698:CDS | 40.0% |
| !! | ATTGCTTTGGTTAGCTTGTG+TGG | + | chr7.3:49156963-49156982 | MS.gene051698:CDS | 40.0% |
| !! | TAGTGATGGTGTTATGGAGT+TGG | + | chr7.3:49157032-49157051 | MS.gene051698:CDS | 40.0% |
| !! | TAGTTCTGGGAATTTGGATG+CGG | + | chr7.3:49157068-49157087 | MS.gene051698:CDS | 40.0% |
| !! | TATGGATTGGGCACATTTCA+TGG | + | chr7.3:49156880-49156899 | MS.gene051698:CDS | 40.0% |
| !! | TTCTTGTTGTTGGGGTTTGT+GGG | + | chr7.3:49157841-49157860 | MS.gene051698:CDS | 40.0% |
| ACATTTCATGGGAAGCCTTC+AGG | + | chr7.3:49156892-49156911 | MS.gene051698:CDS | 45.0% | |
| AGGTGTGGTTAGTGTTATGG+GGG | - | chr7.3:49156447-49156466 | None:intergenic | 45.0% | |
| ATTTCGCGGAAGGATGAAAC+GGG | - | chr7.3:49156403-49156422 | None:intergenic | 45.0% | |
| CATTTCATGGGAAGCCTTCA+GGG | + | chr7.3:49156893-49156912 | MS.gene051698:CDS | 45.0% | |
| CCTCTCGTGCACAAATTGAT+CGG | - | chr7.3:49156995-49157014 | None:intergenic | 45.0% | |
| CGGATTCGAGAATACGGAAA+AGG | - | chr7.3:49156357-49156376 | None:intergenic | 45.0% | |
| CTCATCCTCATAAGCAATCC+TGG | - | chr7.3:49157145-49157164 | None:intergenic | 45.0% | |
| CTGCGGATTCTAAACTGTTG+GGG | + | chr7.3:49157706-49157725 | MS.gene051698:CDS | 45.0% | |
| GAAGTGGAAGGAAGTTTCGA+TGG | + | chr7.3:49157667-49157686 | MS.gene051698:CDS | 45.0% | |
| GAGGTGTGGTTAGTGTTATG+GGG | - | chr7.3:49156448-49156467 | None:intergenic | 45.0% | |
| GATCTGTGCTTGTATGTCTC+AGG | + | chr7.3:49157806-49157825 | MS.gene051698:CDS | 45.0% | |
| GCATTAGCAAGAGCAGTAAG+AGG | - | chr7.3:49156667-49156686 | None:intergenic | 45.0% | |
| GCCATAAACAACATCAGCAG+CGG | - | chr7.3:49157457-49157476 | None:intergenic | 45.0% | |
| GCTCAAAGATGGGATGCAAA+AGG | + | chr7.3:49157578-49157597 | MS.gene051698:CDS | 45.0% | |
| GTCTGCGGATTCTAAACTGT+TGG | + | chr7.3:49157704-49157723 | MS.gene051698:CDS | 45.0% | |
| TCCATCTCCATCTGCAGAAA+TGG | - | chr7.3:49156794-49156813 | None:intergenic | 45.0% | |
| TCCATTTCTGCAGATGGAGA+TGG | + | chr7.3:49156790-49156809 | MS.gene051698:CDS | 45.0% | |
| TTCGTCCAGGATTGCTTATG+AGG | + | chr7.3:49157137-49157156 | MS.gene051698:CDS | 45.0% | |
| ! | AACTGTTGTTGGCTAGGATG+GGG | + | chr7.3:49157274-49157293 | MS.gene051698:CDS | 45.0% |
| ! | AGGTTCAGAGGGCAATTTTG+AGG | + | chr7.3:49157607-49157626 | MS.gene051698:CDS | 45.0% |
| ! | GAAACTGTTGTTGGCTAGGA+TGG | + | chr7.3:49157272-49157291 | MS.gene051698:CDS | 45.0% |
| ! | GGAGGTGTGGTTAGTGTTAT+GGG | - | chr7.3:49156449-49156468 | None:intergenic | 45.0% |
| ! | GGATGTGGTGGAAGAAAGTT+GGG | - | chr7.3:49156278-49156297 | None:intergenic | 45.0% |
| ! | TCCGCTGCTGATGTTGTTTA+TGG | + | chr7.3:49157453-49157472 | MS.gene051698:CDS | 45.0% |
| ! | TGGAGGTGTGGTTAGTGTTA+TGG | - | chr7.3:49156450-49156469 | None:intergenic | 45.0% |
| !! | AAGAAAGTTGGGCGGATTGA+CGG | - | chr7.3:49156267-49156286 | None:intergenic | 45.0% |
| !! | AGATGAAGAGCGTCAAGATC+CGG | + | chr7.3:49156825-49156844 | MS.gene051698:CDS | 45.0% |
| !! | CGTGGGTTTTTGAGGATTCA+TGG | + | chr7.3:49157414-49157433 | MS.gene051698:CDS | 45.0% |
| !! | GTTCTTGTTGTTGGGGTTTG+TGG | + | chr7.3:49157840-49157859 | MS.gene051698:CDS | 45.0% |
| ACGAGAGGTTGAGTGATGAG+AGG | + | chr7.3:49157007-49157026 | MS.gene051698:CDS | 50.0% | |
| CCGATCAATTTGTGCACGAG+AGG | + | chr7.3:49156992-49157011 | MS.gene051698:CDS | 50.0% | |
| CTCTGACTCAGACTCCGAAT+CGG | + | chr7.3:49156720-49156739 | MS.gene051698:CDS | 50.0% | |
| CTGCTCAAGGAAATGCGTTC+GGG | + | chr7.3:49157880-49157899 | MS.gene051698:CDS | 50.0% | |
| GAGTCGTTCGTGAATTCGGA+TGG | + | chr7.3:49157492-49157511 | MS.gene051698:CDS | 50.0% | |
| GCTGCTCAAGGAAATGCGTT+CGG | + | chr7.3:49157879-49157898 | MS.gene051698:CDS | 50.0% | |
| GGTGTGGTTAGTGTTATGGG+GGG | - | chr7.3:49156446-49156465 | None:intergenic | 50.0% | |
| GGTTGAGGGGAAAATGAGGA+CGG | - | chr7.3:49156302-49156321 | None:intergenic | 50.0% | |
| GTTGAAGCTTGACGAGTCTG+CGG | + | chr7.3:49157689-49157708 | MS.gene051698:CDS | 50.0% | |
| GTTGAGGGGAAAATGAGGAC+GGG | - | chr7.3:49156301-49156320 | None:intergenic | 50.0% | |
| TCCTGCAACAGCATCAGCTT+CGG | - | chr7.3:49157168-49157187 | None:intergenic | 50.0% | |
| TCTGCAGATGGAGATGGAGA+TGG | + | chr7.3:49156796-49156815 | MS.gene051698:CDS | 50.0% | |
| TGCTCAAGGAAATGCGTTCG+GGG | + | chr7.3:49157881-49157900 | MS.gene051698:CDS | 50.0% | |
| TGGTTAGTGTTATGGGGGGT+GGG | - | chr7.3:49156442-49156461 | None:intergenic | 50.0% | |
| TTGAGGGGAAAATGAGGACG+GGG | - | chr7.3:49156300-49156319 | None:intergenic | 50.0% | |
| TTTCGCGGAAGGATGAAACG+GGG | - | chr7.3:49156402-49156421 | None:intergenic | 50.0% | |
| ! | CTTTGAGAGAAAAGGGTGCG+AGG | + | chr7.3:49157772-49157791 | MS.gene051698:CDS | 50.0% |
| ! | GGGATGTGGTGGAAGAAAGT+TGG | - | chr7.3:49156279-49156298 | None:intergenic | 50.0% |
| ! | TGTGGTGGAAGAAAGTTGGG+CGG | - | chr7.3:49156275-49156294 | None:intergenic | 50.0% |
| ! | TTATGGCGTGACTGCGCTTT+TGG | + | chr7.3:49157470-49157489 | MS.gene051698:CDS | 50.0% |
| ! | TTGTTGTTGGGGTTTGTGGG+AGG | + | chr7.3:49157844-49157863 | MS.gene051698:CDS | 50.0% |
| !!! | AAAGGGTTTTGAGTGCGCAG+AGG | - | chr7.3:49156339-49156358 | None:intergenic | 50.0% |
| !!! | AAGGGTTTTGAGTGCGCAGA+GGG | - | chr7.3:49156338-49156357 | None:intergenic | 50.0% |
| !!! | CAGAGGGCAATTTTGAGGCA+GGG | + | chr7.3:49157612-49157631 | MS.gene051698:CDS | 50.0% |
| !!! | TCAGAGGGCAATTTTGAGGC+AGG | + | chr7.3:49157611-49157630 | MS.gene051698:CDS | 50.0% |
| AATCGACGTCGGAGGTTGAG+GGG | - | chr7.3:49156315-49156334 | None:intergenic | 55.0% | |
| AGCTGATGCTGTTGCAGGAG+TGG | + | chr7.3:49157169-49157188 | MS.gene051698:CDS | 55.0% | |
| CGGAGGTTGAGGGGAAAATG+AGG | - | chr7.3:49156306-49156325 | None:intergenic | 55.0% | |
| CGGGGTTGCGTTTAGAAGTG+CGG | + | chr7.3:49157899-49157918 | MS.gene051698:CDS | 55.0% | |
| GAAAATGAGGACGGGGGATG+TGG | - | chr7.3:49156293-49156312 | None:intergenic | 55.0% | |
| GAATCGACGTCGGAGGTTGA+GGG | - | chr7.3:49156316-49156335 | None:intergenic | 55.0% | |
| GTGGTTAGTGTTATGGGGGG+TGG | - | chr7.3:49156443-49156462 | None:intergenic | 55.0% | |
| TGAGGGGAAAATGAGGACGG+GGG | - | chr7.3:49156299-49156318 | None:intergenic | 55.0% | |
| ! | TGCGTTTAGAAGTGCGGCTG+AGG | + | chr7.3:49157905-49157924 | MS.gene051698:CDS | 55.0% |
| !! | TTAGGGCACCGAATTCGTCC+AGG | + | chr7.3:49157124-49157143 | MS.gene051698:CDS | 55.0% |
| AATGAGGACGGGGGATGTGG+TGG | - | chr7.3:49156290-49156309 | None:intergenic | 60.0% | |
| CCTCGTTTGGCTGCTGCTCA+AGG | + | chr7.3:49157867-49157886 | MS.gene051698:CDS | 60.0% | |
| CCTTGAGCAGCAGCCAAACG+AGG | - | chr7.3:49157870-49157889 | None:intergenic | 60.0% | |
| GCCGAAGCTGATGCTGTTGC+AGG | + | chr7.3:49157164-49157183 | MS.gene051698:CDS | 60.0% | |
| GGAATCGACGTCGGAGGTTG+AGG | - | chr7.3:49156317-49156336 | None:intergenic | 60.0% | |
| GGTTTGTGGGAGGCCTCGTT+TGG | + | chr7.3:49157854-49157873 | MS.gene051698:CDS | 60.0% | |
| TGCGCAGAGGGAATCGACGT+CGG | - | chr7.3:49156326-49156345 | None:intergenic | 60.0% | |
| GCAGAGGGAATCGACGTCGG+AGG | - | chr7.3:49156323-49156342 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 49156223 | 49158031 | 49156223 | ID=MS.gene051698 |
| chr7.3 | mRNA | 49156223 | 49158031 | 49156223 | ID=MS.gene051698.t1;Parent=MS.gene051698 |
| chr7.3 | exon | 49156223 | 49158031 | 49156223 | ID=MS.gene051698.t1.exon1;Parent=MS.gene051698.t1 |
| chr7.3 | CDS | 49156223 | 49158031 | 49156223 | ID=cds.MS.gene051698.t1;Parent=MS.gene051698.t1 |
| Gene Sequence |
| Protein sequence |
