Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48326.t1 | KEH31776.1 | 80.3 | 127 | 15 | 1 | 37 | 153 | 11 | 137 | 9.40E-47 | 196.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48326.t1 | Q9SB64 | 49.4 | 81 | 41 | 0 | 49 | 129 | 12 | 92 | 1.5e-13 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48326.t1 | A0A072UQZ7 | 80.3 | 127 | 15 | 1 | 37 | 153 | 11 | 137 | 6.8e-47 | 196.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene48326 | 0.818355 | 2.05E-52 | -1.69E-46 |
| MS.gene049915 | MS.gene48326 | 0.825983 | 3.48E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene48326 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene48326 | 0.811237 | 7.74E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene48326 | -0.800641 | 1.31E-48 | -1.69E-46 |
| MS.gene050642 | MS.gene48326 | 0.801806 | 7.57E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene48326 | 0.811912 | 5.52E-51 | -1.69E-46 |
| MS.gene051633 | MS.gene48326 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene48326 | 0.812508 | 4.09E-51 | -1.69E-46 |
| MS.gene051663 | MS.gene48326 | 0.800136 | 1.66E-48 | -1.69E-46 |
| MS.gene051789 | MS.gene48326 | 0.800676 | 1.29E-48 | -1.69E-46 |
| MS.gene052286 | MS.gene48326 | 0.81791 | 2.58E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene48326 | 0.821923 | 3.12E-53 | -1.69E-46 |
| MS.gene052376 | MS.gene48326 | 0.820807 | 5.64E-53 | -1.69E-46 |
| MS.gene052600 | MS.gene48326 | 0.80124 | 9.88E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene48326 | 0.809826 | 1.56E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene48326 | 0.804389 | 2.21E-49 | -1.69E-46 |
| MS.gene054667 | MS.gene48326 | 0.808936 | 2.42E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene48326 | 0.839486 | 1.54E-57 | -1.69E-46 |
| MS.gene055441 | MS.gene48326 | 0.800467 | 1.42E-48 | -1.69E-46 |
| MS.gene057260 | MS.gene48326 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene057754 | MS.gene48326 | 0.857435 | 1.64E-62 | -1.69E-46 |
| MS.gene058182 | MS.gene48326 | 0.808136 | 3.59E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene48326 | 0.817956 | 2.52E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene48326 | 0.827647 | 1.39E-54 | -1.69E-46 |
| MS.gene060297 | MS.gene48326 | 0.814995 | 1.16E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene48326 | 0.819718 | 1.00E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48326.t1 | MTR_4g104890 | 76.087 | 138 | 22 | 2 | 26 | 153 | 1 | 137 | 4.24e-56 | 174 |
| MS.gene48326.t1 | MTR_4g104960 | 55.357 | 112 | 44 | 2 | 47 | 153 | 18 | 128 | 1.63e-34 | 119 |
| MS.gene48326.t1 | MTR_4g104900 | 56.140 | 114 | 44 | 2 | 44 | 152 | 4 | 116 | 2.13e-33 | 116 |
| MS.gene48326.t1 | MTR_4g104980 | 68.182 | 110 | 29 | 2 | 49 | 153 | 29 | 137 | 4.99e-33 | 115 |
| MS.gene48326.t1 | MTR_4g104940 | 54.955 | 111 | 44 | 2 | 49 | 153 | 9 | 119 | 6.40e-32 | 112 |
| MS.gene48326.t1 | MTR_4g104990 | 64.151 | 106 | 29 | 3 | 49 | 153 | 24 | 121 | 3.61e-27 | 100 |
| MS.gene48326.t1 | MTR_4g104950 | 68.539 | 89 | 24 | 1 | 58 | 146 | 3 | 87 | 7.12e-22 | 85.5 |
| MS.gene48326.t1 | MTR_4g104880 | 66.667 | 87 | 29 | 0 | 44 | 130 | 4 | 90 | 9.01e-22 | 90.9 |
| MS.gene48326.t1 | MTR_4g104880 | 66.667 | 87 | 29 | 0 | 44 | 130 | 4 | 90 | 9.43e-22 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48326.t1 | AT4G24690 | 49.383 | 81 | 41 | 0 | 49 | 129 | 12 | 92 | 2.19e-19 | 84.0 |
Find 21 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGTTGTTCACACTTTC+TGG | 0.076406 | 8.3:+17690179 | MS.gene48326:CDS |
| TCTACTGATGTCATACTTGC+TGG | 0.316535 | 8.3:+17690237 | MS.gene48326:CDS |
| ACTTGCTGGCTGTTCATACT+TGG | 0.360207 | 8.3:+17690251 | MS.gene48326:CDS |
| TTGAGAATTGAAGTACAATA+TGG | 0.400819 | 8.3:+17691201 | MS.gene48326:CDS |
| TCTTAACATGGTTGAGTTGA+GGG | 0.421418 | 8.3:+17691044 | MS.gene48326:CDS |
| AATCTGTGTGGAAAATATTA+CGG | 0.435313 | 8.3:+17691259 | MS.gene48326:CDS |
| CCTTCCTGTGATGGAATGGC+TGG | 0.453910 | 8.3:+17690162 | None:intergenic |
| GCTGGCTGTTCATACTTGGC+TGG | 0.485154 | 8.3:+17690255 | MS.gene48326:CDS |
| ACGAACATTGAAGCGCTTGA+AGG | 0.493686 | 8.3:-17690998 | None:intergenic |
| TGTGGATGAGAATAATCAAA+TGG | 0.500589 | 8.3:+17691020 | MS.gene48326:CDS |
| TGTTGATGAAGAGGGTGACT+TGG | 0.514717 | 8.3:+17691122 | MS.gene48326:CDS |
| ATCTTAACATGGTTGAGTTG+AGG | 0.541915 | 8.3:+17691043 | MS.gene48326:CDS |
| TTGAGTTGAGGGCTAAGATA+AGG | 0.584743 | 8.3:+17691055 | MS.gene48326:CDS |
| TGTGTGGAAAATATTACGGA+AGG | 0.616681 | 8.3:+17691263 | MS.gene48326:CDS |
| TAATCAAATGGATCTTAACA+TGG | 0.622269 | 8.3:+17691032 | MS.gene48326:CDS |
| ATGATTTGCACGACGTGATG+AGG | 0.626481 | 8.3:+17691163 | MS.gene48326:CDS |
| CAAGCGCTTCAATGTTCGTG+TGG | 0.628729 | 8.3:+17691002 | MS.gene48326:CDS |
| CTGAGATATGTTGATGAAGA+GGG | 0.631119 | 8.3:+17691114 | MS.gene48326:CDS |
| ATTGAAGTACAATATGGCAA+CGG | 0.647428 | 8.3:+17691207 | MS.gene48326:CDS |
| TCTGAGATATGTTGATGAAG+AGG | 0.660807 | 8.3:+17691113 | MS.gene48326:CDS |
| AAATGATCGGTAAATCTGTG+TGG | 0.665580 | 8.3:+17691247 | MS.gene48326:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAATTACTATGTTTTTT+TGG | + | chr8.3:17690930-17690949 | MS.gene48326:intron | 10.0% |
| !! | AAAATTATGTACCAAATATT+TGG | - | chr8.3:17690698-17690717 | None:intergenic | 15.0% |
| !! | TATTTCAAAATCCAAATATT+TGG | + | chr8.3:17690684-17690703 | MS.gene48326:intron | 15.0% |
| !! | TTTCAATTGAGAAAAATGAT+CGG | + | chr8.3:17691234-17691253 | MS.gene48326:CDS | 20.0% |
| !!! | AAATGCTTAAAAAGTATCTT+TGG | - | chr8.3:17690785-17690804 | None:intergenic | 20.0% |
| !!! | AATGCTTAAAAAGTATCTTT+GGG | - | chr8.3:17690784-17690803 | None:intergenic | 20.0% |
| !!! | ATCTTATTTCAGTTAAAGTA+AGG | - | chr8.3:17690654-17690673 | None:intergenic | 20.0% |
| !!! | GCATTTTTCAAATTCAATTT+TGG | + | chr8.3:17690799-17690818 | MS.gene48326:intron | 20.0% |
| !!! | TATTTGGATTTTGAAATAGT+AGG | - | chr8.3:17690682-17690701 | None:intergenic | 20.0% |
| ! | AATCTGTGTGGAAAATATTA+CGG | + | chr8.3:17691259-17691278 | MS.gene48326:CDS | 25.0% |
| ! | CAGTAATAAAGCTTCAAAAA+AGG | - | chr8.3:17690210-17690229 | None:intergenic | 25.0% |
| ! | TAATCAAATGGATCTTAACA+TGG | + | chr8.3:17691032-17691051 | MS.gene48326:CDS | 25.0% |
| ! | TTGAGAATTGAAGTACAATA+TGG | + | chr8.3:17691201-17691220 | MS.gene48326:CDS | 25.0% |
| !!! | ACTCATTTTAGAGTTAGTTA+AGG | + | chr8.3:17690487-17690506 | MS.gene48326:intron | 25.0% |
| !!! | ATCTTGTTTTTGTTAGTAGT+TGG | + | chr8.3:17690283-17690302 | MS.gene48326:CDS | 25.0% |
| !!! | ATTTTTGGGGTCAAAATTTT+GGG | - | chr8.3:17690749-17690768 | None:intergenic | 25.0% |
| !!! | CTCATTTTAGAGTTAGTTAA+GGG | + | chr8.3:17690488-17690507 | MS.gene48326:intron | 25.0% |
| !!! | CTTTTTTGAAGCTTTATTAC+TGG | + | chr8.3:17690208-17690227 | MS.gene48326:CDS | 25.0% |
| AAAATAGAGAGACCAAAAGT+GGG | + | chr8.3:17690866-17690885 | MS.gene48326:intron | 30.0% | |
| ATTGAAGAGCAAATTCTGTT+TGG | + | chr8.3:17690329-17690348 | MS.gene48326:intron | 30.0% | |
| ATTGAAGTACAATATGGCAA+CGG | + | chr8.3:17691207-17691226 | MS.gene48326:CDS | 30.0% | |
| TAAAATAGAGAGACCAAAAG+TGG | + | chr8.3:17690865-17690884 | MS.gene48326:intron | 30.0% | |
| TGTGGATGAGAATAATCAAA+TGG | + | chr8.3:17691020-17691039 | MS.gene48326:CDS | 30.0% | |
| ! | GACAAAAATCCACATTTTTG+GGG | - | chr8.3:17690762-17690781 | None:intergenic | 30.0% |
| ! | GGACAAAAATCCACATTTTT+GGG | - | chr8.3:17690763-17690782 | None:intergenic | 30.0% |
| ! | TATGGTTTGAAGCTTTGTAT+TGG | + | chr8.3:17690363-17690382 | MS.gene48326:intron | 30.0% |
| ! | TGGTCATTTCTTTTTCTGTT+TGG | + | chr8.3:17690383-17690402 | MS.gene48326:intron | 30.0% |
| ! | TTCACATATCGTTGCAATTT+TGG | + | chr8.3:17690716-17690735 | MS.gene48326:intron | 30.0% |
| !! | AAATTTTGACCCCAAAAATG+TGG | + | chr8.3:17690750-17690769 | MS.gene48326:intron | 30.0% |
| !!! | CATTTTTGGGGTCAAAATTT+TGG | - | chr8.3:17690750-17690769 | None:intergenic | 30.0% |
| AAATGATCGGTAAATCTGTG+TGG | + | chr8.3:17691247-17691266 | MS.gene48326:CDS | 35.0% | |
| ATCATTTGTGAACTCTGTAC+AGG | + | chr8.3:17690960-17690979 | MS.gene48326:intron | 35.0% | |
| TGTGTGGAAAATATTACGGA+AGG | + | chr8.3:17691263-17691282 | MS.gene48326:CDS | 35.0% | |
| TGTTTGGAAGCTTTCTCTTA+TGG | + | chr8.3:17690345-17690364 | MS.gene48326:intron | 35.0% | |
| TTCTCTTACCTTGAGTTTGA+GGG | + | chr8.3:17690439-17690458 | MS.gene48326:intron | 35.0% | |
| ! | ATCTTAACATGGTTGAGTTG+AGG | + | chr8.3:17691043-17691062 | MS.gene48326:CDS | 35.0% |
| ! | CTGAGATATGTTGATGAAGA+GGG | + | chr8.3:17691114-17691133 | MS.gene48326:CDS | 35.0% |
| ! | GGGACAAAAATCCACATTTT+TGG | - | chr8.3:17690764-17690783 | None:intergenic | 35.0% |
| ! | TCTGAGATATGTTGATGAAG+AGG | + | chr8.3:17691113-17691132 | MS.gene48326:CDS | 35.0% |
| ! | TGGGGCTAAAATCTTGTTTA+AGG | + | chr8.3:17690842-17690861 | MS.gene48326:intron | 35.0% |
| !! | GCTTTTTCTCTCAATGTTCA+TGG | + | chr8.3:17690609-17690628 | MS.gene48326:intron | 35.0% |
| !! | TAACTAGCTTAACCCACTTT+TGG | - | chr8.3:17690881-17690900 | None:intergenic | 35.0% |
| !! | TCTTAACATGGTTGAGTTGA+GGG | + | chr8.3:17691044-17691063 | MS.gene48326:CDS | 35.0% |
| !!! | AATTCAATTTTGGAGCAACC+TGG | + | chr8.3:17690809-17690828 | MS.gene48326:intron | 35.0% |
| !!! | AGCAACCTGGATTTTGAAAT+TGG | + | chr8.3:17690822-17690841 | MS.gene48326:intron | 35.0% |
| !!! | CAACCTGGATTTTGAAATTG+GGG | + | chr8.3:17690824-17690843 | MS.gene48326:intron | 35.0% |
| !!! | GCAACCTGGATTTTGAAATT+GGG | + | chr8.3:17690823-17690842 | MS.gene48326:intron | 35.0% |
| GTTCTCTTACCTTGAGTTTG+AGG | + | chr8.3:17690438-17690457 | MS.gene48326:intron | 40.0% | |
| TAGCCCCAATTTCAAAATCC+AGG | - | chr8.3:17690830-17690849 | None:intergenic | 40.0% | |
| TCTACTGATGTCATACTTGC+TGG | + | chr8.3:17690237-17690256 | MS.gene48326:CDS | 40.0% | |
| !! | TTGAGTTGAGGGCTAAGATA+AGG | + | chr8.3:17691055-17691074 | MS.gene48326:CDS | 40.0% |
| TCTTACCTTGAGTTTGAGGG+AGG | + | chr8.3:17690442-17690461 | MS.gene48326:intron | 45.0% | |
| ! | ACGAACATTGAAGCGCTTGA+AGG | - | chr8.3:17691001-17691020 | None:intergenic | 45.0% |
| ! | ACTTGCTGGCTGTTCATACT+TGG | + | chr8.3:17690251-17690270 | MS.gene48326:CDS | 45.0% |
| ! | ATGATTTGCACGACGTGATG+AGG | + | chr8.3:17691163-17691182 | MS.gene48326:CDS | 45.0% |
| ! | TGTTGATGAAGAGGGTGACT+TGG | + | chr8.3:17691122-17691141 | MS.gene48326:CDS | 45.0% |
| AACAGCCTCCCTCAAACTCA+AGG | - | chr8.3:17690450-17690469 | None:intergenic | 50.0% | |
| CAAGCGCTTCAATGTTCGTG+TGG | + | chr8.3:17691002-17691021 | MS.gene48326:CDS | 50.0% | |
| ! | GGCTGGTTGTTCACACTTTC+TGG | + | chr8.3:17690179-17690198 | MS.gene48326:CDS | 50.0% |
| GCTGGCTGTTCATACTTGGC+TGG | + | chr8.3:17690255-17690274 | MS.gene48326:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 17690177 | 17691299 | 17690177 | ID=MS.gene48326 |
| chr8.3 | mRNA | 17690177 | 17691299 | 17690177 | ID=MS.gene48326.t1;Parent=MS.gene48326 |
| chr8.3 | exon | 17690177 | 17690320 | 17690177 | ID=MS.gene48326.t1.exon1;Parent=MS.gene48326.t1 |
| chr8.3 | CDS | 17690177 | 17690320 | 17690177 | ID=cds.MS.gene48326.t1;Parent=MS.gene48326.t1 |
| chr8.3 | exon | 17690982 | 17691299 | 17690982 | ID=MS.gene48326.t1.exon2;Parent=MS.gene48326.t1 |
| chr8.3 | CDS | 17690982 | 17691299 | 17690982 | ID=cds.MS.gene48326.t1;Parent=MS.gene48326.t1 |
| Gene Sequence |
| Protein sequence |
