AlfalfaGEDB | MS.gene50859

M. sativa cultivar XinJiangDaYe / MS.gene50859

KEGG: K01061
GO: GO:0003674 GO:0003824 GO:0016787
PFAM: PF01738.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50859.t1	XP_003597994.1	96.7	273	9	0	11	283	61	333	6.90E-152	546.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50859.t1	Q7TP52	26.6	237	151	8	56	282	22	245	1.8e-20	101.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50859.t1	G7IYB4	96.7	273	9	0	11	283	61	333	4.9e-152	546.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050404	MS.gene50859	0.844296	8.26E-59	-1.69E-46
MS.gene050406	MS.gene50859	0.848352	6.48E-60	-1.69E-46
MS.gene050529	MS.gene50859	0.809249	2.08E-50	-1.69E-46
MS.gene051171	MS.gene50859	0.88691	2.27E-72	-1.69E-46
MS.gene051208	MS.gene50859	0.821546	3.81E-53	-1.69E-46
MS.gene051240	MS.gene50859	0.841844	3.71E-58	-1.69E-46
MS.gene051242	MS.gene50859	0.886274	3.95E-72	-1.69E-46
MS.gene051244	MS.gene50859	0.871781	5.26E-67	-1.69E-46
MS.gene051582	MS.gene50859	0.827755	1.31E-54	-1.69E-46
MS.gene052134	MS.gene50859	0.832423	9.53E-56	-1.69E-46
MS.gene052135	MS.gene50859	0.815085	1.11E-51	-1.69E-46
MS.gene053330	MS.gene50859	0.910032	2.95E-82	-1.69E-46
MS.gene053907	MS.gene50859	0.810716	1.00E-50	-1.69E-46
MS.gene053971	MS.gene50859	0.869326	3.37E-66	-1.69E-46
MS.gene055370	MS.gene50859	0.884271	2.21E-71	-1.69E-46
MS.gene05553	MS.gene50859	0.813423	2.58E-51	-1.69E-46
MS.gene05625	MS.gene50859	0.801975	6.99E-49	-1.69E-46
MS.gene056305	MS.gene50859	0.827104	1.88E-54	-1.69E-46
MS.gene056351	MS.gene50859	0.823531	1.32E-53	-1.69E-46
MS.gene056451	MS.gene50859	0.827464	1.54E-54	-1.69E-46
MS.gene056633	MS.gene50859	0.937866	1.72E-98	-1.69E-46
MS.gene056664	MS.gene50859	0.882382	1.09E-70	-1.69E-46
MS.gene056796	MS.gene50859	0.809811	1.57E-50	-1.69E-46
MS.gene057196	MS.gene50859	0.866822	2.15E-65	-1.69E-46
MS.gene057332	MS.gene50859	0.818794	1.63E-52	-1.69E-46
MS.gene058233	MS.gene50859	0.871182	8.30E-67	-1.69E-46
MS.gene058276	MS.gene50859	0.86048	2.01E-63	-1.69E-46
MS.gene058743	MS.gene50859	0.808752	2.65E-50	-1.69E-46
MS.gene058746	MS.gene50859	0.99243	3.07E-193	-1.69E-46
MS.gene059512	MS.gene50859	0.862989	3.43E-64	-1.69E-46
MS.gene059894	MS.gene50859	0.800429	1.45E-48	-1.69E-46
MS.gene060407	MS.gene50859	0.84069	7.47E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50859.t1	MTR_3g005060	96.703	273	9	0	11	283	61	333	0.0	550
MS.gene50859.t1	MTR_3g005060	96.169	261	10	0	23	283	59	319	3.25e-178	494
MS.gene50859.t1	MTR_1g052490	74.286	105	25	2	105	207	7	111	4.57e-51	164
MS.gene50859.t1	MTR_4g117450	27.053	207	133	6	84	282	42	238	1.59e-18	82.8
MS.gene50859.t1	MTR_2g021340	27.358	212	139	6	78	282	37	240	1.98e-18	82.8
MS.gene50859.t1	MTR_4g117400	25.524	286	181	9	6	282	30	292	2.56e-18	83.2
MS.gene50859.t1	MTR_2g021340	27.358	212	139	6	78	282	40	243	2.63e-18	82.4
MS.gene50859.t1	MTR_4g117380	29.817	218	142	6	68	282	28	237	3.38e-16	76.3
MS.gene50859.t1	MTR_4g117450	25.463	216	136	6	84	282	42	249	2.76e-15	73.9
MS.gene50859.t1	MTR_4g117450	26.178	191	121	6	101	282	2	181	3.94e-14	69.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50859.t1	AT1G35420	60.256	234	89	1	50	283	45	274	7.06e-111	322
MS.gene50859.t1	AT1G35420	60.256	234	89	1	50	283	81	310	1.57e-110	322
MS.gene50859.t1	AT1G35420	60.256	234	89	1	50	283	86	315	2.42e-110	322
MS.gene50859.t1	AT3G23600	25.000	200	136	4	84	277	42	233	1.06e-13	69.3
MS.gene50859.t1	AT3G23570	23.832	214	143	6	78	282	36	238	5.99e-13	67.4
MS.gene50859.t1	AT3G23570	23.832	214	143	6	78	282	36	238	5.99e-13	67.4
MS.gene50859.t1	AT3G23570	23.832	214	143	6	78	282	36	238	5.99e-13	67.4
MS.gene50859.t1	AT3G23600	24.500	200	134	4	84	277	42	230	1.18e-12	66.6
MS.gene50859.t1	AT2G32520	25.926	270	156	13	36	283	30	277	1.46e-12	66.6
MS.gene50859.t1	AT2G32520	26.022	269	155	13	36	282	70	316	1.87e-12	67.0
MS.gene50859.t1	AT2G32520	25.703	249	147	12	57	283	7	239	4.20e-12	65.1
MS.gene50859.t1	AT2G32520	26.522	230	134	11	76	283	9	225	7.67e-12	63.9

Find 0 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTAAAAAATAACAATGGAAT+TGG	+	21095:93286-93305	MS.gene50859:CDS	20.0%
!	AAGGCAGTAAAAAATAACAA+TGG	+	21095:93280-93299	MS.gene50859:CDS	25.0%
!	TAAATAAATATGCCTAAGAG+AGG	-	21095:93456-93475	None:intergenic	25.0%
!!!	ATGTTATTTTATCCTCTCTT+AGG	+	21095:93441-93460	MS.gene50859:intron	25.0%
	AAACATCAGAAAGAAGCAAT+AGG	-	21095:93312-93331	None:intergenic	30.0%
	ATCATGTAGTTTACTGAATG+TGG	+	21095:93165-93184	MS.gene50859:CDS	30.0%
	ATCTATCCTTGTACCATAAA+AGG	-	21095:93758-93777	None:intergenic	30.0%
	ATTCAGACTACAATTGATAG+TGG	+	21095:93865-93884	MS.gene50859:CDS	30.0%
	TGCTTCTTTCTGATGTTTAT+GGG	+	21095:93314-93333	MS.gene50859:CDS	30.0%
	TTGCTTCTTTCTGATGTTTA+TGG	+	21095:93313-93332	MS.gene50859:CDS	30.0%
!	TGTTCATGCTTATCTTTTCA+AGG	+	21095:93261-93280	MS.gene50859:CDS	30.0%
!!!	ATAATTGGTTTTTGCTTTGG+AGG	+	21095:93673-93692	MS.gene50859:CDS	30.0%
!!!	TTCCCGTCTTGTTTATTTTA+GGG	+	21095:93803-93822	MS.gene50859:CDS	30.0%
	AGACTGGATACATGAACATT+TGG	+	21095:93990-94009	MS.gene50859:CDS	35.0%
	ATATGCAAAATCTCTTGTGG+AGG	-	21095:93350-93369	None:intergenic	35.0%
	CAAACACTCAGTTTGAACAA+TGG	+	21095:93548-93567	MS.gene50859:CDS	35.0%
	CAGTATGTTGAAGATAGCTA+GGG	-	21095:93409-93428	None:intergenic	35.0%
	CATTGTTCAAACTGAGTGTT+TGG	-	21095:93550-93569	None:intergenic	35.0%
	CCAGTCTCTCATAATTACAT+AGG	-	21095:93977-93996	None:intergenic	35.0%
	CCTATGTAATTATGAGAGAC+TGG	+	21095:93974-93993	MS.gene50859:CDS	35.0%
	CTGAAGAATTGCATTCAACA+AGG	-	21095:93078-93097	None:intergenic	35.0%
	GTCCCCTAAAATAAACAAGA+CGG	-	21095:93809-93828	None:intergenic	35.0%
	TCCAGAAAACTTGGCATAAT+TGG	+	21095:93658-93677	MS.gene50859:CDS	35.0%
	TCCCCTAAAATAAACAAGAC+GGG	-	21095:93808-93827	None:intergenic	35.0%
	TTCTGATGTTTATGGGTTTG+AGG	+	21095:93321-93340	MS.gene50859:CDS	35.0%
!	ACCAATTATGCCAAGTTTTC+TGG	-	21095:93662-93681	None:intergenic	35.0%
!	CACAAGAGATTTTGCATATC+GGG	+	21095:93351-93370	MS.gene50859:CDS	35.0%
!	CCACAAGAGATTTTGCATAT+CGG	+	21095:93350-93369	MS.gene50859:CDS	35.0%
!	GAATCTCCTTTTATGGTACA+AGG	+	21095:93749-93768	MS.gene50859:CDS	35.0%
!!!	GGCATAATTGGTTTTTGCTT+TGG	+	21095:93670-93689	MS.gene50859:CDS	35.0%
!!!	GTTCCCGTCTTGTTTATTTT+AGG	+	21095:93802-93821	MS.gene50859:CDS	35.0%
!!!	TCCCGTCTTGTTTATTTTAG+GGG	+	21095:93804-93823	MS.gene50859:CDS	35.0%
	AAATCAGTTCAAGATACCGC+AGG	+	21095:93133-93152	MS.gene50859:CDS	40.0%
	ACCTGGGAATATCACTACTT+CGG	-	21095:93893-93912	None:intergenic	40.0%
	ACTTATTTCGCGGAAATCCA+TGG	+	21095:93512-93531	MS.gene50859:CDS	40.0%
	ATGTGAATTAGTCAGTGGAG+AGG	+	21095:93210-93229	MS.gene50859:CDS	40.0%
	ATTTCACTTACAGCGCAAAG+TGG	-	21095:93838-93857	None:intergenic	40.0%
	CCAGTATGTTGAAGATAGCT+AGG	-	21095:93410-93429	None:intergenic	40.0%
	CCGATATGCAAAATCTCTTG+TGG	-	21095:93353-93372	None:intergenic	40.0%
	CCTAGCTATCTTCAACATAC+TGG	+	21095:93407-93426	MS.gene50859:intron	40.0%
	CTACTTCCAGACTTATTTCG+CGG	+	21095:93502-93521	MS.gene50859:CDS	40.0%
	GAAGGAGTTTCCAGAAAACT+TGG	+	21095:93649-93668	MS.gene50859:CDS	40.0%
	GATGCATGTGAATTAGTCAG+TGG	+	21095:93205-93224	MS.gene50859:CDS	40.0%
	TGTTGAAGATAGCTAGGGTA+TGG	-	21095:93404-93423	None:intergenic	40.0%
	TTTCACTTACAGCGCAAAGT+GGG	-	21095:93837-93856	None:intergenic	40.0%
!	AAAGCAAGCATCTTGGTCTT+GGG	-	21095:93722-93741	None:intergenic	40.0%
!	ACATGATACTTTTGAGCCTG+CGG	-	21095:93152-93171	None:intergenic	40.0%
!	ATGTCTTTTGCAATCCTCTC+AGG	-	21095:93589-93608	None:intergenic	40.0%
!	GCAGATGAATTGATGACTGA+AGG	+	21095:93631-93650	MS.gene50859:CDS	40.0%
!	TGTCTTTTGCAATCCTCTCA+GGG	-	21095:93588-93607	None:intergenic	40.0%
!!	CAGTTTGAACAATGGATTGC+TGG	+	21095:93556-93575	MS.gene50859:CDS	40.0%
!!	GAGGAGCTTTATTTAGAGGA+TGG	+	21095:93229-93248	MS.gene50859:CDS	40.0%
!!	TGGAGAGGAGCTTTATTTAG+AGG	+	21095:93225-93244	MS.gene50859:CDS	40.0%
!!!	ATTGGTTTTTGCTTTGGAGG+AGG	+	21095:93676-93695	MS.gene50859:CDS	40.0%
	ATATTCCCAGGTAGAGGTCA+TGG	+	21095:93901-93920	MS.gene50859:CDS	45.0%
	CAAAGCAAGCATCTTGGTCT+TGG	-	21095:93723-93742	None:intergenic	45.0%
	CAAATCCATGACCTCTACCT+GGG	-	21095:93909-93928	None:intergenic	45.0%
	CAAGACCAAGATGCTTGCTT+TGG	+	21095:93721-93740	MS.gene50859:CDS	45.0%
	GGATTTCCGCGAAATAAGTC+TGG	-	21095:93511-93530	None:intergenic	45.0%
	GTAGTGATATTCCCAGGTAG+AGG	+	21095:93895-93914	MS.gene50859:CDS	45.0%
	GTTCAAACTGAGTGTTTGGC+TGG	-	21095:93546-93565	None:intergenic	45.0%
	TCCGAAGTAGTGATATTCCC+AGG	+	21095:93889-93908	MS.gene50859:CDS	45.0%
	TGGATCTGTGGAAGAAGACA+AGG	+	21095:93939-93958	MS.gene50859:CDS	45.0%
	TTGCAAAAGACATTACCGCG+TGG	+	21095:93596-93615	MS.gene50859:CDS	45.0%
	TTGTCTTCTTCCACAGATCC+AGG	-	21095:93940-93959	None:intergenic	45.0%
!!	GGCACTGGAATCTCCTTTTA+TGG	+	21095:93742-93761	MS.gene50859:CDS	45.0%
	ACATTACCGCGTGGACAGAA+TGG	+	21095:93605-93624	MS.gene50859:CDS	50.0%
	CAAGATGCTTGCTTTGGCAC+TGG	+	21095:93727-93746	MS.gene50859:CDS	50.0%
	CAGTGCCAAAGCAAGCATCT+TGG	-	21095:93729-93748	None:intergenic	50.0%
	CTTCTTCCACAGATCCAGGT+CGG	-	21095:93936-93955	None:intergenic	50.0%
	GCAAATCCATGACCTCTACC+TGG	-	21095:93910-93929	None:intergenic	50.0%
	TCGCGGAAATCCATGGACAA+AGG	+	21095:93519-93538	MS.gene50859:CDS	50.0%
	TTGCTGGACATAACCCTGAG+AGG	+	21095:93572-93591	MS.gene50859:CDS	50.0%
!	GCATATCGGGTAGCTTGCAA+TGG	+	21095:93364-93383	MS.gene50859:CDS	50.0%
!	TGGAGGAGGCAAAGTGTTAG+AGG	+	21095:93690-93709	MS.gene50859:CDS	50.0%
!	TTTGGCTGGTCCTTTGTCCA+TGG	-	21095:93532-93551	None:intergenic	50.0%
	TTCCACAGATCCAGGTCGGT+GGG	-	21095:93932-93951	None:intergenic	55.0%
	CATGGATTTGCCCACCGACC+TGG	+	21095:93919-93938	MS.gene50859:CDS	60.0%
	CTTCCACAGATCCAGGTCGG+TGG	-	21095:93933-93952	None:intergenic	60.0%
	TACCGCGTGGACAGAATGGC+TGG	+	21095:93609-93628	MS.gene50859:CDS	60.0%
	TGCCAGCCATTCTGTCCACG+CGG	-	21095:93614-93633	None:intergenic	60.0%
	TGCCCACCGACCTGGATCTG+TGG	+	21095:93927-93946	MS.gene50859:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
21095	gene	93061	94017	93061	ID=MS.gene50859
21095	mRNA	93061	94017	93061	ID=MS.gene50859.t1;Parent=MS.gene50859
21095	exon	93061	93392	93061	ID=MS.gene50859.t1.exon1;Parent=MS.gene50859.t1
21095	CDS	93061	93392	93061	ID=cds.MS.gene50859.t1;Parent=MS.gene50859.t1
21095	exon	93498	94017	93498	ID=MS.gene50859.t1.exon2;Parent=MS.gene50859.t1
21095	CDS	93498	94017	93498	ID=cds.MS.gene50859.t1;Parent=MS.gene50859.t1

Gene Sequence

>MS.gene50859

ATGCACTATGTTTACCTTGTTGAATGCAATTCTTCAGTTTGTTTTCAGAAAAAAAGCAAATTGATTCTTCAAAAATCAGTTCAAGATACCGCAGGCTCAAAAGTATCATGTAGTTTACTGAATGTGGAAAGTGTGATAGATGATGATGCATGTGAATTAGTCAGTGGAGAGGAGCTTTATTTAGAGGATGGCGATGATAATGTTCATGCTTATCTTTTCAAGGCAGTAAAAAATAACAATGGAATTGGCCTATTGCTTCTTTCTGATGTTTATGGGTTTGAGGACTCCTCCACAAGAGATTTTGCATATCGGGTAGCTTGCAATGGTTTCAAGTACGTCTCCATACCCTAGCTATCTTCAACATACTGGTTTCAAAGTGTATGTTATTTTATCCTCTCTTAGGCATATTTATTTACTTCTTCTACACAAATTTGCAGCGTTCTACTTCCAGACTTATTTCGCGGAAATCCATGGACAAAGGACCAGCCAAACACTCAGTTTGAACAATGGATTGCTGGACATAACCCTGAGAGGATTGCAAAAGACATTACCGCGTGGACAGAATGGCTGGCAGATGAATTGATGACTGAAGGAGTTTCCAGAAAACTTGGCATAATTGGTTTTTGCTTTGGAGGAGGCAAAGTGTTAGAGGTGCTAGCCCAAGACCAAGATGCTTGCTTTGGCACTGGAATCTCCTTTTATGGTACAAGGATAGATTCGCTTGTTGCTTCTGATGTAAAAGTTCCCGTCTTGTTTATTTTAGGGGACAATGACCCACTTTGCGCTGTAAGTGAAATAAAAAATATTCAGACTACAATTGATAGTGGTTCCGAAGTAGTGATATTCCCAGGTAGAGGTCATGGATTTGCCCACCGACCTGGATCTGTGGAAGAAGACAAGGACGCTGAACAAGCCTATGTAATTATGAGAGACTGGATACATGAACATTTGGTGTAA

Protein sequence

>MS.gene50859.t1

MHYVYLVECNSSVCFQKKSKLILQKSVQDTAGSKVSCSLLNVESVIDDDACELVSGEELYLEDGDDNVHAYLFKAVKNNNGIGLLLLSDVYGFEDSSTRDFAYRVACNGFNVLLPDLFRGNPWTKDQPNTQFEQWIAGHNPERIAKDITAWTEWLADELMTEGVSRKLGIIGFCFGGGKVLEVLAQDQDACFGTGISFYGTRIDSLVASDVKVPVLFILGDNDPLCAVSEIKNIQTTIDSGSEVVIFPGRGHGFAHRPGSVEEDKDAEQAYVIMRDWIHEHLV