AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056451


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCACTTGTTACTCTTAC+AGG 0.198423 8.3:+32951758 MS.gene056451:CDS
CTTCTAGTGCCTTTATGCAT+TGG 0.385653 8.3:-32951721 None:intergenic
AGATCATGCATCTCTCTATC+AGG 0.411660 8.3:-32951682 None:intergenic
TTTACGTACGTGAACGAGCT+TGG 0.432955 8.3:-32951941 None:intergenic
ACAAACAAGGAAGAGGGTAT+TGG 0.476829 8.3:-32951478 None:intergenic
GAGTCAAGACCAATGCATAA+AGG 0.483045 8.3:+32951712 MS.gene056451:CDS
CGTACGTGAACGAGCTTGGA+GGG 0.493662 8.3:-32951937 None:intergenic
TCCACATAGCTATATTAGAA+TGG 0.532785 8.3:+32951508 MS.gene056451:CDS
ACGTACGTGAACGAGCTTGG+AGG 0.547000 8.3:-32951938 None:intergenic
TGACAATACAAACAAGGAAG+AGG 0.554518 8.3:-32951485 None:intergenic
AAGGCACTAGAAGAACATGC+AGG 0.583674 8.3:+32951731 MS.gene056451:CDS
AGTGTGGAGAGAGTTGCAAA+AGG 0.597912 8.3:+32951875 MS.gene056451:intron
GACAATACAAACAAGGAAGA+GGG 0.614485 8.3:-32951484 None:intergenic
AGGCACTAGAAGAACATGCA+GGG 0.629832 8.3:+32951732 MS.gene056451:CDS
ACCATTCTAATATAGCTATG+TGG 0.637830 8.3:-32951509 None:intergenic
AGACCTGTAAGAGTAACAAG+TGG 0.663372 8.3:-32951761 None:intergenic
TGTGGATGACAATACAAACA+AGG 0.679966 8.3:-32951491 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAATATTATTATTTTA+TGG + chr8.3:32951587-32951606 MS.gene056451:intron 0.0%
!! TTATTATGATATTGAAAACA+AGG - chr8.3:32951622-32951641 None:intergenic 15.0%
! TGAAACTTATGTTACACTTT+GGG + chr8.3:32951810-32951829 MS.gene056451:intron 25.0%
! TTGAAACTTATGTTACACTT+TGG + chr8.3:32951809-32951828 MS.gene056451:intron 25.0%
ACCATTCTAATATAGCTATG+TGG - chr8.3:32951512-32951531 None:intergenic 30.0%
TCCACATAGCTATATTAGAA+TGG + chr8.3:32951508-32951527 MS.gene056451:CDS 30.0%
TTTCATGCATGGAAAAATGT+AGG + chr8.3:32951652-32951671 MS.gene056451:intron 30.0%
! TACACTTTGGGAAACATATA+TGG + chr8.3:32951822-32951841 MS.gene056451:intron 30.0%
! TGTATATTCATTTTGCAGTG+TGG + chr8.3:32951859-32951878 MS.gene056451:intron 30.0%
! TTTTCCATGCATGAAAGTTT+TGG - chr8.3:32951648-32951667 None:intergenic 30.0%
GACAATACAAACAAGGAAGA+GGG - chr8.3:32951487-32951506 None:intergenic 35.0%
TGACAATACAAACAAGGAAG+AGG - chr8.3:32951488-32951507 None:intergenic 35.0%
TGCATTGTGCTTAATTCTGT+TGG - chr8.3:32951446-32951465 None:intergenic 35.0%
TGTGGATGACAATACAAACA+AGG - chr8.3:32951494-32951513 None:intergenic 35.0%
AGACCTGTAAGAGTAACAAG+TGG - chr8.3:32951764-32951783 None:intergenic 40.0%
AGATCATGCATCTCTCTATC+AGG - chr8.3:32951685-32951704 None:intergenic 40.0%
AGCTCCAAAACTTTCATGCA+TGG + chr8.3:32951641-32951660 MS.gene056451:intron 40.0%
CTTCTAGTGCCTTTATGCAT+TGG - chr8.3:32951724-32951743 None:intergenic 40.0%
GAGTCAAGACCAATGCATAA+AGG + chr8.3:32951712-32951731 MS.gene056451:CDS 40.0%
! ACAAACAAGGAAGAGGGTAT+TGG - chr8.3:32951481-32951500 None:intergenic 40.0%
AGGCACTAGAAGAACATGCA+GGG + chr8.3:32951732-32951751 MS.gene056451:CDS 45.0%
AGTGTGGAGAGAGTTGCAAA+AGG + chr8.3:32951875-32951894 MS.gene056451:intron 45.0%
! AAGGCACTAGAAGAACATGC+AGG + chr8.3:32951731-32951750 MS.gene056451:CDS 45.0%
! CAGCCACTTGTTACTCTTAC+AGG + chr8.3:32951758-32951777 MS.gene056451:CDS 45.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 32951444 32951954 32951444 ID=MS.gene056451
chr8.3 mRNA 32951444 32951954 32951444 ID=MS.gene056451.t1;Parent=MS.gene056451
chr8.3 exon 32951444 32951529 32951444 ID=MS.gene056451.t1.exon1;Parent=MS.gene056451.t1
chr8.3 CDS 32951444 32951529 32951444 ID=cds.MS.gene056451.t1;Parent=MS.gene056451.t1
chr8.3 exon 32951674 32951779 32951674 ID=MS.gene056451.t1.exon2;Parent=MS.gene056451.t1
chr8.3 CDS 32951674 32951779 32951674 ID=cds.MS.gene056451.t1;Parent=MS.gene056451.t1
chr8.3 exon 32951877 32951954 32951877 ID=MS.gene056451.t1.exon3;Parent=MS.gene056451.t1
chr8.3 CDS 32951877 32951954 32951877 ID=cds.MS.gene056451.t1;Parent=MS.gene056451.t1
Gene Sequence

>MS.gene056451

CAACAGAATTAAGCACAATGCATGATCACACTCACCAATACCCTCTTCCTTGTTTGTATTGTCATCCACATAGCTATATTAGAATGGTAATATTAAGCTACTATATTATAATATACATGTTGAGCACTTTTTATAATTTACTTTAAAAAATATTATTATTTTATGGTATGTGTTTCCTTGTTTTCAATATCATAATAAGCTCCAAAACTTTCATGCATGGAAAAATGTAGGTTCAAAACCTGATAGAGAGATGCATGATCTTTCACATGAGTCAAGACCAATGCATAAAGGCACTAGAAGAACATGCAGGGATTCAGCCACTTGTTACTCTTACAGGTCTTTTGATTATAGTCTTTTCATTAGTCTTGAAACTTATGTTACACTTTGGGAAACATATATGGTTATGTGAATTAAATGTATATTCATTTTGCAGTGTGGAGAGAGTTGCAAAAGGAGAATGAAGAGTTCTTTAGAGCATATTTGCACAATTTATCCCTCCAAGCTCGTTCAC

Protein sequence

>MS.gene056451.t1

QQNUAQCMITLTNTLFLVCIVIHIAILEWFKTUURDAUSFTUVKTNAURHUKNMQGFSHLLLLQCGESCKRRMKSSLEHICTIYPSKLVH