AlfalfaGEDB | MS.gene53496

M. sativa cultivar XinJiangDaYe / MS.gene53496

KEGG: K03294
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF13520.9 PF13906.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53496.t1	XP_003630015.1	96.5	604	21	0	1	604	1	604	0.00E+00	1140.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53496.t1	Q84MA5	67.2	592	184	1	11	602	9	590	1.4e-230	800.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53496.t1	G7L9L7	96.5	604	21	0	1	604	1	604	0.0e+00	1140.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08148	MS.gene53496	PPI
MS.gene042491	MS.gene53496	PPI
MS.gene53496	MS.gene073450	PPI
MS.gene53496	MS.gene007812	PPI
MS.gene53496	MS.gene00402	PPI
MS.gene49831	MS.gene53496	PPI
MS.gene53496	MS.gene08144	PPI
MS.gene41790	MS.gene53496	PPI
MS.gene007811	MS.gene53496	PPI
MS.gene41795	MS.gene53496	PPI
MS.gene41792	MS.gene53496	PPI
MS.gene53496	MS.gene041367	PPI
MS.gene53496	MS.gene08137	PPI
MS.gene38965	MS.gene53496	PPI
MS.gene38964	MS.gene53496	PPI
MS.gene53496	MS.gene32277	PPI
MS.gene53496	MS.gene47297	PPI
MS.gene042480	MS.gene53496	PPI
MS.gene58674	MS.gene53496	PPI
MS.gene032361	MS.gene53496	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53496.t1	MTR_8g089320	96.523	604	21	0	1	604	1	604	0.0	1189
MS.gene53496.t1	MTR_8g089360	75.261	574	138	3	21	594	26	595	0.0	880
MS.gene53496.t1	MTR_8g089360	75.261	574	138	3	21	594	9	578	0.0	880
MS.gene53496.t1	MTR_8g089340	91.685	457	37	1	149	604	1	457	0.0	810
MS.gene53496.t1	MTR_1g030660	63.276	580	206	2	28	601	23	601	0.0	760
MS.gene53496.t1	MTR_2g035430	56.559	587	255	0	1	587	1	587	0.0	707
MS.gene53496.t1	MTR_8g461380	50.000	568	278	2	19	586	26	587	0.0	577
MS.gene53496.t1	MTR_1g111740	40.986	588	324	4	28	603	6	582	6.38e-152	450
MS.gene53496.t1	MTR_4g099270	39.671	547	312	6	51	587	1	539	3.22e-126	383
MS.gene53496.t1	MTR_4g099270	44.385	374	204	2	35	404	6	379	2.43e-105	323
MS.gene53496.t1	MTR_4g099270	37.471	435	254	6	163	587	1	427	6.03e-89	283
MS.gene53496.t1	MTR_4g099270	43.709	302	166	2	35	332	6	307	3.09e-82	261
MS.gene53496.t1	MTR_8g089342	92.086	139	10	1	1	138	1	139	5.16e-81	251
MS.gene53496.t1	MTR_6g027630	35.000	420	263	5	35	446	7	424	3.46e-75	251
MS.gene53496.t1	MTR_6g027630	35.000	420	263	5	35	446	7	424	7.54e-75	251
MS.gene53496.t1	MTR_7g086510	36.829	391	240	3	63	446	29	419	8.40e-75	251
MS.gene53496.t1	MTR_6g027680	35.385	390	243	3	65	446	39	427	1.52e-71	241
MS.gene53496.t1	MTR_6g034535	43.657	268	136	5	328	586	49	310	9.68e-71	231
MS.gene53496.t1	MTR_6g034535	43.750	96	29	1	98	193	19	89	4.63e-15	77.0
MS.gene53496.t1	MTR_6g027660	32.401	429	277	4	25	446	3	425	9.82e-71	240
MS.gene53496.t1	MTR_6g027650	35.233	386	241	4	69	446	33	417	1.33e-70	239
MS.gene53496.t1	MTR_7g086610	35.770	383	237	3	72	446	32	413	1.37e-69	237
MS.gene53496.t1	MTR_7g007850	30.769	520	340	9	65	571	42	554	1.42e-67	230
MS.gene53496.t1	MTR_7g007850	28.909	550	341	9	65	571	42	584	4.43e-63	219
MS.gene53496.t1	MTR_7g007850	32.524	412	267	7	65	467	42	451	5.28e-60	208
MS.gene53496.t1	MTR_7g007850	32.524	412	267	7	65	467	42	451	1.03e-59	208
MS.gene53496.t1	MTR_6g027630	34.118	340	215	4	115	446	1	339	2.87e-55	196
MS.gene53496.t1	MTR_6g027670	32.278	158	107	0	289	446	1	158	2.47e-25	107
MS.gene53496.t1	MTR_2g064760	89.655	29	3	0	489	517	13	41	5.64e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53496.t1	AT4G21120	67.003	594	186	1	11	604	9	592	0.0	837
MS.gene53496.t1	AT2G34960	57.651	562	236	2	11	571	3	563	0.0	679
MS.gene53496.t1	AT4G21120	63.938	452	153	1	153	604	1	442	0.0	604
MS.gene53496.t1	AT1G17120	53.488	559	259	1	13	571	22	579	0.0	595
MS.gene53496.t1	AT3G10600	40.273	586	316	8	27	592	11	582	6.48e-145	432
MS.gene53496.t1	AT5G04770	40.034	582	317	7	30	592	15	583	7.96e-144	429
MS.gene53496.t1	AT5G04770	41.667	384	214	2	30	403	15	398	3.25e-106	327
MS.gene53496.t1	AT3G10600	38.222	450	244	8	163	592	1	436	2.81e-100	312
MS.gene53496.t1	AT5G04770	38.085	449	246	7	163	592	1	436	9.51e-93	292
MS.gene53496.t1	AT5G36940	35.417	384	241	3	70	446	27	410	1.29e-73	247
MS.gene53496.t1	AT3G03720	34.663	401	253	3	53	446	7	405	1.27e-72	244
MS.gene53496.t1	AT1G58030	33.766	385	248	3	69	446	37	421	1.82e-68	234
MS.gene53496.t1	AT1G05940	29.511	532	352	7	64	578	40	565	7.08e-67	228
MS.gene53496.t1	AT1G05940	30.242	496	323	7	64	542	40	529	6.70e-64	219
MS.gene53496.t1	AT1G05940	31.382	427	280	5	64	478	40	465	1.27e-61	213
MS.gene53496.t1	AT1G05940	27.703	444	300	5	151	578	9	447	8.33e-44	162

Find 160 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATTGGAATTTCAGCTTAT+TGG	0.122057	4.2:+12251571	MS.gene53496:CDS
TCCACGACTTCACTCCTTTC+GGG	0.194112	4.2:+12250932	MS.gene53496:CDS
CTTGTATACTATGTGCTATT+GGG	0.197270	4.2:+12251819	MS.gene53496:CDS
GTGTGTCTTGTGTTGATTAT+TGG	0.202436	4.2:+12251543	MS.gene53496:CDS
CCTTGTATACTATGTGCTAT+TGG	0.216779	4.2:+12251818	MS.gene53496:CDS
GCTATTAACATATTCCTTCT+TGG	0.218446	4.2:+12251744	MS.gene53496:CDS
TTTGTTCCTCTGTGGTTTAT+AGG	0.224891	4.2:+12251630	MS.gene53496:CDS
AATTGGAATTTCAGCTTATT+GGG	0.231227	4.2:+12251572	MS.gene53496:CDS
TTGTTCCTCTGTGGTTTATA+GGG	0.252540	4.2:+12251631	MS.gene53496:CDS
GTTGCTTTCTTTACCGATCT+TGG	0.269135	4.2:+12251396	MS.gene53496:CDS
TTCGGCATCGGCGCCGTTAT+TGG	0.280711	4.2:+12248542	MS.gene53496:CDS
TTATAGGGACTGGTGCTCTT+TGG	0.283313	4.2:+12251646	MS.gene53496:CDS
TGCTTACTTAAGAGTAGAAT+TGG	0.292424	4.2:+12250585	MS.gene53496:CDS
TGCAGAGCTGGTGGAGATTA+AGG	0.299520	4.2:+12248466	MS.gene53496:CDS
CAAGGGCTGTGATGGCTACT+AGG	0.303231	4.2:-12250795	None:intergenic
TTCACTCCTTTCGGGCTTCA+TGG	0.308522	4.2:+12250940	MS.gene53496:CDS
GGGGAGTACCTTTGGTTCCT+TGG	0.311642	4.2:+12251703	MS.gene53496:CDS
TTCCACGACTTCACTCCTTT+CGG	0.314152	4.2:+12250931	MS.gene53496:CDS
ATACTCAAGGAGGATGTTTC+CGG	0.316964	4.2:-12250632	None:intergenic
GGGCATATCTGCTTTGTTAT+CGG	0.324019	4.2:+12248643	MS.gene53496:CDS
TGTATTGTCGTTTGTCATCT+CGG	0.339399	4.2:+12248622	MS.gene53496:CDS
GACATTCCAATTGGTCTAGT+TGG	0.355410	4.2:+12251054	MS.gene53496:CDS
TCAAAAGAACGACTTCTTCC+CGG	0.355424	4.2:+12248349	MS.gene53496:CDS
CACAATATGAACCCTGACTA+TGG	0.355787	4.2:+12250745	MS.gene53496:CDS
CGTATATAAGGTTTGCGGTT+TGG	0.357696	4.2:+12251784	MS.gene53496:CDS
GCTGTGGAAATTCCGGTTGC+AGG	0.357996	4.2:+12248686	MS.gene53496:CDS
TCTGGAATATTTGTGCTTAC+CGG	0.360075	4.2:+12248566	MS.gene53496:CDS
GCAATTGAACAAGGTGGAAC+AGG	0.361594	4.2:+12251920	MS.gene53496:CDS
TTGATTATTGGTTCTTCAAT+TGG	0.371036	4.2:+12251555	MS.gene53496:CDS
TTGCTTTCTTTACCGATCTT+GGG	0.377778	4.2:+12251397	MS.gene53496:CDS
ACTAGGGCTGCAATGGCAAT+AGG	0.381476	4.2:-12250778	None:intergenic
TAAGTTATGGGGAGTACCTT+TGG	0.384691	4.2:+12251695	MS.gene53496:CDS
TAGTGCTGTTGGAATGGATT+GGG	0.389974	4.2:+12251182	MS.gene53496:CDS
GGGTCAAGATGGCCATAGTC+AGG	0.395099	4.2:-12250757	None:intergenic
TCCTTTCGGGCTTCATGGTA+TGG	0.395161	4.2:+12250945	MS.gene53496:CDS
GGAGGAGTCGTTTAAGAGTT+GGG	0.398486	4.2:+12248370	MS.gene53496:CDS
GGCAAAGAAGCCTAAGTTAT+GGG	0.403530	4.2:+12251683	MS.gene53496:CDS
ATTGAGATTGTAGCATTCAT+AGG	0.404896	4.2:-12251354	None:intergenic
TCAAGTGAGGTAACTACAAA+AGG	0.408616	4.2:+12251501	MS.gene53496:CDS
AACTTAGGCTTCTTTGCCAT+TGG	0.410038	4.2:-12251678	None:intergenic
ATCGGCGCCGTTATTGGTTC+TGG	0.410639	4.2:+12248548	MS.gene53496:CDS
CTGTCATGGAAACACCTTAC+CGG	0.410920	4.2:+12248408	MS.gene53496:CDS
AGAGTTTGCTGTGGAAATTC+CGG	0.413761	4.2:+12248679	MS.gene53496:CDS
TGGCATTGGCACTTCTAGTA+AGG	0.430198	4.2:+12251469	MS.gene53496:CDS
GGCTAGGCAGGAAGCAGGTC+CGG	0.432589	4.2:+12248595	MS.gene53496:CDS
GATGTTTCCGGCAGCTATAA+AGG	0.433876	4.2:-12250620	None:intergenic
ACTATTATACGAAAATCATC+AGG	0.434415	4.2:-12250721	None:intergenic
GCTCTTGTTGATGAAAGAAC+AGG	0.435974	4.2:+12251327	MS.gene53496:CDS
ATAAGGTTTGCGGTTTGGAC+AGG	0.438702	4.2:+12251789	MS.gene53496:CDS
ATTGAACCAACTAGACCAAT+TGG	0.438801	4.2:-12251060	None:intergenic
ACATACTAGTGGTTCTGATC+AGG	0.442761	4.2:+12251887	MS.gene53496:CDS
TTAGTGCTGTTGGAATGGAT+TGG	0.443178	4.2:+12251181	MS.gene53496:CDS
GTATTGTCGTTTGTCATCTC+GGG	0.449106	4.2:+12248623	MS.gene53496:CDS
CGGAGGAGTCGTTTAAGAGT+TGG	0.471701	4.2:+12248369	MS.gene53496:CDS
TACCGGACTTGAGGCTAGGC+AGG	0.477948	4.2:+12248583	MS.gene53496:CDS
GCACTAAAAGCAACTGAGAA+AGG	0.478246	4.2:-12251165	None:intergenic
CCTCTGTGGTTTATAGGGAC+TGG	0.479549	4.2:+12251636	MS.gene53496:CDS
TGTGGCTATGTAGTTGAAGA+TGG	0.479563	4.2:-12250842	None:intergenic
AACAGTGAGAATGGATGGAT+TGG	0.479583	4.2:+12251597	MS.gene53496:CDS
GTTGAAGATGGAAGAGCCTT+TGG	0.481809	4.2:-12250830	None:intergenic
GCTTACTTAAGAGTAGAATT+GGG	0.482784	4.2:+12250586	MS.gene53496:CDS
CAACTCTTAAACGACTCCTC+CGG	0.483055	4.2:-12248368	None:intergenic
GTTCTTAGTTTCTTCAGCCA+TGG	0.485412	4.2:-12251022	None:intergenic
AACTCTTAAACGACTCCTCC+GGG	0.489247	4.2:-12248367	None:intergenic
TGGCAAAGAAGCCTAAGTTA+TGG	0.489518	4.2:+12251682	MS.gene53496:CDS
GACTTGATGTGGTTCGGCAT+CGG	0.491036	4.2:+12248530	MS.gene53496:CDS
TGGTGGGACTTGATGTGGTT+CGG	0.493050	4.2:+12248524	MS.gene53496:CDS
ACGCCGCGGTAGCTCGATCA+TGG	0.494424	4.2:+12250665	MS.gene53496:CDS
AAAATCGTATATAAGGTTTG+CGG	0.499007	4.2:+12251779	MS.gene53496:CDS
GACACACAATGAGATTTACT+TGG	0.500876	4.2:-12251527	None:intergenic
TATTGGGCTAACAGTGAGAA+TGG	0.501880	4.2:+12251588	MS.gene53496:CDS
ATCCTCCTTGAGTATATCAT+AGG	0.504563	4.2:+12250640	MS.gene53496:CDS
CCAATTGGAATGTCTCTACC+AGG	0.509969	4.2:-12251045	None:intergenic
CCTGGTAGAGACATTCCAAT+TGG	0.511803	4.2:+12251045	MS.gene53496:CDS
GGAAACACCTTACCGGTTGA+AGG	0.513323	4.2:+12248415	MS.gene53496:CDS
GGAGAGGTGGTCGATGGCGG+CGG	0.515481	4.2:+12248302	MS.gene53496:CDS
AAGGGCTGTGATGGCTACTA+GGG	0.519818	4.2:-12250794	None:intergenic
TTTGTTGCCTTTATAGCTGC+CGG	0.521495	4.2:+12250613	MS.gene53496:CDS
CTAGGGCTGCAATGGCAATA+GGG	0.522514	4.2:-12250777	None:intergenic
GCAACAAACGATCCTTCAAC+CGG	0.523498	4.2:-12248427	None:intergenic
AAATATTCCAGAACCAATAA+CGG	0.523587	4.2:-12248555	None:intergenic
ATCTAGTGATCATGCAGAGC+TGG	0.523633	4.2:+12248454	MS.gene53496:CDS
CAAGTGAGGTAACTACAAAA+GGG	0.524334	4.2:+12251502	MS.gene53496:CDS
TTAATAGCAATAGATAAAGA+AGG	0.526151	4.2:-12251729	None:intergenic
GGGTTTGGCTTTGATTAGAC+CGG	0.527710	4.2:-12250902	None:intergenic
TTTAAGAGTTGGGGAAACTA+CGG	0.530921	4.2:+12248380	MS.gene53496:CDS
CCAATAGCACATAGTATACA+AGG	0.532930	4.2:-12251818	None:intergenic
AGATGAAGAAGACGTTGAAC+TGG	0.533887	4.2:+12248504	MS.gene53496:CDS
CTTGCAGTTTACAGCACCAA+AGG	0.534562	4.2:+12250814	MS.gene53496:CDS
TTGTATACTATGTGCTATTG+GGG	0.543861	4.2:+12251820	MS.gene53496:CDS
CTTTGATGCAGTTTCAACCA+TGG	0.552516	4.2:+12251005	MS.gene53496:CDS
CTTGAGGCTAGGCAGGAAGC+AGG	0.555643	4.2:+12248590	MS.gene53496:CDS
TGCTTACCGGACTTGAGGCT+AGG	0.556310	4.2:+12248579	MS.gene53496:CDS
GGGCTAACAGTGAGAATGGA+TGG	0.558502	4.2:+12251592	MS.gene53496:CDS
ATAGATAAAGAAGGTAGCCA+AGG	0.559440	4.2:-12251720	None:intergenic
AAACGATCCTTCAACCGGTA+AGG	0.560163	4.2:-12248422	None:intergenic
TAAGGTTTGCGGTTTGGACA+GGG	0.562484	4.2:+12251790	MS.gene53496:CDS
CAATGGCAATAGGGTCAAGA+TGG	0.562907	4.2:-12250768	None:intergenic
GTAAACTGCAAGGGCTGTGA+TGG	0.563586	4.2:-12250803	None:intergenic
CATAGTCAGGGTTCATATTG+TGG	0.563878	4.2:-12250744	None:intergenic
GTTGGAGAGGTGGTCGATGG+CGG	0.564645	4.2:+12248299	MS.gene53496:CDS
GAAAACAGGAACATGGCAGT+TGG	0.566836	4.2:+12248281	None:intergenic
CTTTGGTGCTGTAAACTGCA+AGG	0.567605	4.2:-12250813	None:intergenic
GCAGTTGGAGAGGTGGTCGA+TGG	0.568853	4.2:+12248296	MS.gene53496:CDS
TAGTGATCATGCAGAGCTGG+TGG	0.569903	4.2:+12248457	MS.gene53496:CDS
GTTTCACTAGGTGCTTTGAA+AGG	0.570706	4.2:+12251216	MS.gene53496:CDS
ACACCATCTTTGTTCCTCTG+TGG	0.571390	4.2:+12251622	MS.gene53496:CDS
ATTTGTGCTTACCGGACTTG+AGG	0.572133	4.2:+12248574	MS.gene53496:CDS
TGAAGAAGACGTTGAACTGG+TGG	0.575728	4.2:+12248507	MS.gene53496:CDS
CAGGAACATGGCAGTTGGAG+AGG	0.576698	4.2:+12248286	None:intergenic
AACATAAGCAAAGAAAAGTA+CGG	0.578928	4.2:-12250980	None:intergenic
GCTAAGTACATTGTTTCACT+AGG	0.579305	4.2:+12251204	MS.gene53496:CDS
GATCAGATTAAACATACTAG+TGG	0.585592	4.2:+12251876	MS.gene53496:CDS
AAGGAGATATTATTCAAGTG+AGG	0.585702	4.2:+12251488	MS.gene53496:CDS
GATGGCTACTAGGGCTGCAA+TGG	0.585884	4.2:-12250785	None:intergenic
TGAACTGGTGGGACTTGATG+TGG	0.588455	4.2:+12248519	MS.gene53496:CDS
TTCCTGCCTAGCCTCAAGTC+CGG	0.590625	4.2:-12248585	None:intergenic
ACCATACCATGAAGCCCGAA+AGG	0.594838	4.2:-12250946	None:intergenic
AAACTACGGAAAATCTGTCA+TGG	0.596565	4.2:+12248394	MS.gene53496:CDS
GCCCGAAAGGAGTGAAGTCG+TGG	0.598740	4.2:-12250933	None:intergenic
GAAGGTAGCCAAGGAACCAA+AGG	0.599611	4.2:-12251711	None:intergenic
GTACTCACATGATGCCACCT+TGG	0.601374	4.2:+12251301	MS.gene53496:CDS
ATTATCTTCATAGTAATCGC+CGG	0.602298	4.2:+12250883	MS.gene53496:CDS
GAGGAGTCGTTTAAGAGTTG+GGG	0.604687	4.2:+12248371	MS.gene53496:CDS
TACTAGTGGTTCTGATCAGG+AGG	0.607435	4.2:+12251890	MS.gene53496:CDS
GAACATGGCAGTTGGAGAGG+TGG	0.607953	4.2:+12248289	None:intergenic
GGTTGAGAAGCAATTGAACA+AGG	0.608119	4.2:+12251911	MS.gene53496:CDS
ACAAAGTAGCAATCGACAAG+AGG	0.610155	4.2:-12251431	None:intergenic
AAACCACAGAGGAACAAAGA+TGG	0.613035	4.2:-12251625	None:intergenic
GTGGAGATTAAGGCTAGGAG+TGG	0.614966	4.2:+12248476	MS.gene53496:CDS
TTTGGTGCTGTAAACTGCAA+GGG	0.618827	4.2:-12250812	None:intergenic
GGTCAAGATGGCCATAGTCA+GGG	0.621031	4.2:-12250756	None:intergenic
TTGTTATACAGAGTTTGCTG+TGG	0.625474	4.2:+12248670	MS.gene53496:CDS
AGCTGGTGGAGATTAAGGCT+AGG	0.626254	4.2:+12248471	MS.gene53496:CDS
GGCGGCGGAGTACGTAGGAG+AGG	0.626720	4.2:+12248317	MS.gene53496:CDS
CAATTGAACAAGGTGGAACA+GGG	0.630668	4.2:+12251921	MS.gene53496:CDS
CGTTGCCTATGATATACTCA+AGG	0.633243	4.2:-12250645	None:intergenic
GTTCGATAGAATCCCAAGAT+CGG	0.634285	4.2:-12251409	None:intergenic
ACAAACGACAATACAACCGC+CGG	0.635158	4.2:-12248614	None:intergenic
CATAGCCACACTCTTACACA+TGG	0.637053	4.2:+12250855	MS.gene53496:CDS
TAGGCAGGAAGCAGGTCCGG+CGG	0.638522	4.2:+12248598	MS.gene53496:CDS
TGCTTTGAAAGGAATGACAA+CGG	0.638848	4.2:+12251227	MS.gene53496:CDS
GCAAAGAAGCCTAAGTTATG+GGG	0.643860	4.2:+12251684	MS.gene53496:CDS
AAAGGTACTCCCCATAACTT+AGG	0.644859	4.2:-12251693	None:intergenic
CTATTATACGAAAATCATCA+GGG	0.647038	4.2:-12250720	None:intergenic
TGGAGATTAAGGCTAGGAGT+GGG	0.659365	4.2:+12248477	MS.gene53496:CDS
GAAGAAGACGTTGAACTGGT+GGG	0.664861	4.2:+12248508	MS.gene53496:CDS
GCTGAAGAAACTAAGAACCC+TGG	0.666018	4.2:+12251027	MS.gene53496:CDS
GGTCCATGATCGAGCTACCG+CGG	0.681679	4.2:-12250668	None:intergenic
TGAGAAGCAATTGAACAAGG+TGG	0.692961	4.2:+12251914	MS.gene53496:CDS
CAATTGGAATGTCTCTACCA+GGG	0.700717	4.2:-12251044	None:intergenic
GCAGAGGGTGGCGAAATACG+AGG	0.702759	4.2:-12250689	None:intergenic
TCGATGGCGGCGGAGTACGT+AGG	0.703593	4.2:+12248312	MS.gene53496:CDS
TGCCTATGATATACTCAAGG+AGG	0.704194	4.2:-12250642	None:intergenic
GACAGCCATGTGTAAGAGTG+TGG	0.708211	4.2:-12250860	None:intergenic
TCAACAAGAGCAAACCAAGG+TGG	0.728106	4.2:-12251315	None:intergenic
AAAGAACGACTTCTTCCCGG+AGG	0.732358	4.2:+12248352	MS.gene53496:CDS
TCATCAACAAGAGCAAACCA+AGG	0.735762	4.2:-12251318	None:intergenic
GTATATCATAGGCAACGCCG+CGG	0.783643	4.2:+12250651	MS.gene53496:CDS
CCAGTCCCTATAAACCACAG+AGG	0.796817	4.2:-12251636	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATATAAATATAAATTAA+AGG	-	chr4.2:12249847-12249866	None:intergenic	0.0%
!!!	ATAATTTTATTTTTTTATTA+TGG	+	chr4.2:12249753-12249772	MS.gene53496:intron	0.0%
!!	ATATAAAGATTATCAATAAA+AGG	-	chr4.2:12250062-12250081	None:intergenic	10.0%
!!!	ATAATTTTTTGTTTACTTTT+AGG	-	chr4.2:12248839-12248858	None:intergenic	10.0%
!!!	ATTTGCATTAAATTTAATTT+AGG	+	chr4.2:12248758-12248777	MS.gene53496:intron	10.0%
!!!	CAAGTTTTTTTTTTTAATAT+TGG	+	chr4.2:12250517-12250536	MS.gene53496:intron	10.0%
!!!	TTATATTTTGAAGAAAAATA+AGG	+	chr4.2:12249565-12249584	MS.gene53496:intron	10.0%
!!!	TTTGCATTAAATTTAATTTA+GGG	+	chr4.2:12248759-12248778	MS.gene53496:intron	10.0%
!!	AAAAAAATTGTATTGAATGA+TGG	-	chr4.2:12249523-12249542	None:intergenic	15.0%
!!	ATATTGTGGAATTATAAATT+TGG	-	chr4.2:12249163-12249182	None:intergenic	15.0%
!!	ATTAAGACAATAAAATGTTA+AGG	+	chr4.2:12250214-12250233	MS.gene53496:intron	15.0%
!!	TATTGTGGAATTATAAATTT+GGG	-	chr4.2:12249162-12249181	None:intergenic	15.0%
!!	TGAAATTATTAAGAATTTGT+TGG	-	chr4.2:12249474-12249493	None:intergenic	15.0%
!!	TTGATAAAAAATCGTATATA+AGG	+	chr4.2:12251772-12251791	MS.gene53496:CDS	15.0%
!!	TTTAAGTCTATAAAAAAATG+TGG	+	chr4.2:12249317-12249336	MS.gene53496:intron	15.0%
!!!	AACAATATTGACAATATTTT+AGG	+	chr4.2:12248957-12248976	MS.gene53496:intron	15.0%
!!!	TATATTCTTTTTGTGATTTA+GGG	+	chr4.2:12250352-12250371	MS.gene53496:intron	15.0%
!!	AAAAGTTGAATTCTCAATTA+TGG	-	chr4.2:12248934-12248953	None:intergenic	20.0%
!!	AAAGATTATCAATAAAAGGT+TGG	-	chr4.2:12250058-12250077	None:intergenic	20.0%
!!	AAAGTTGAATTCTCAATTAT+GGG	-	chr4.2:12248933-12248952	None:intergenic	20.0%
!!	AAATTAATTAATATGCATCG+AGG	-	chr4.2:12249283-12249302	None:intergenic	20.0%
!!	ACAACAAAGAAATAATAGAT+AGG	+	chr4.2:12249097-12249116	MS.gene53496:intron	20.0%
!!	ATACACTATTTATTCCAATA+TGG	-	chr4.2:12249823-12249842	None:intergenic	20.0%
!!	ATCTTATGGTAGTAAAATAT+CGG	-	chr4.2:12249718-12249737	None:intergenic	20.0%
!!	TAATTCCACAATATTAAAAG+TGG	+	chr4.2:12249169-12249188	MS.gene53496:intron	20.0%
!!	TGTGTATTCATGTAAATTAA+AGG	-	chr4.2:12249787-12249806	None:intergenic	20.0%
!!	TTAATAGCAATAGATAAAGA+AGG	-	chr4.2:12251732-12251751	None:intergenic	20.0%
!!!	AACTTGATGTTTTCTTTTAA+GGG	+	chr4.2:12249198-12249217	MS.gene53496:intron	20.0%
!!!	GTATATTCTTTTTGTGATTT+AGG	+	chr4.2:12250351-12250370	MS.gene53496:intron	20.0%
!!!	TAACTTGATGTTTTCTTTTA+AGG	+	chr4.2:12249197-12249216	MS.gene53496:intron	20.0%
!	AAAAAAAAGAAAGGAGTAAG+TGG	-	chr4.2:12249628-12249647	None:intergenic	25.0%
!	AAAATCGTATATAAGGTTTG+CGG	+	chr4.2:12251779-12251798	MS.gene53496:CDS	25.0%
!	AAACGACAACTAAAAAAGAA+CGG	+	chr4.2:12250316-12250335	MS.gene53496:intron	25.0%
!	AAATATTCCAGAACCAATAA+CGG	-	chr4.2:12248558-12248577	None:intergenic	25.0%
!	AACATAAGCAAAGAAAAGTA+CGG	-	chr4.2:12250983-12251002	None:intergenic	25.0%
!	AATATAAAACCCTCCTTATT+TGG	-	chr4.2:12249552-12249571	None:intergenic	25.0%
!	AATTGGAATTTCAGCTTATT+GGG	+	chr4.2:12251572-12251591	MS.gene53496:CDS	25.0%
!	ACACTCAAAAGTTTATAAGT+TGG	-	chr4.2:12248874-12248893	None:intergenic	25.0%
!	ACTATTATACGAAAATCATC+AGG	-	chr4.2:12250724-12250743	None:intergenic	25.0%
!	AGAATCTTAGAGAATTCAAA+AGG	-	chr4.2:12249247-12249266	None:intergenic	25.0%
!	CAAATAAAATTGAACTCTTG+CGG	+	chr4.2:12248896-12248915	MS.gene53496:intron	25.0%
!	CTAAACATTAGTGAATCTTA+TGG	-	chr4.2:12249732-12249751	None:intergenic	25.0%
!	CTATTATACGAAAATCATCA+GGG	-	chr4.2:12250723-12250742	None:intergenic	25.0%
!	GAGAGTTTGAAAAAAAAGAA+AGG	-	chr4.2:12249637-12249656	None:intergenic	25.0%
!	TATAAATTTGGGTGCTTTAT+TGG	-	chr4.2:12249151-12249170	None:intergenic	25.0%
!!	ATATAAAACCCTCCTTATTT+GGG	-	chr4.2:12249551-12249570	None:intergenic	25.0%
!!	ATTGTATTGAATGATGGTTT+TGG	-	chr4.2:12249517-12249536	None:intergenic	25.0%
!!	TTGTATTGAATGATGGTTTT+GGG	-	chr4.2:12249516-12249535	None:intergenic	25.0%
!!	TTTGTTTATCTTTTCACTTG+TGG	+	chr4.2:12251449-12251468	MS.gene53496:CDS	25.0%
!!	TTTTATCAATAGATCCAAGA+AGG	-	chr4.2:12251761-12251780	None:intergenic	25.0%
!!!	AATAGCCACTTTTAATATTG+TGG	-	chr4.2:12249177-12249196	None:intergenic	25.0%
!!!	GCTTTATTGGTTTTAACTTT+GGG	-	chr4.2:12249138-12249157	None:intergenic	25.0%
!!!	TGCTTTATTGGTTTTAACTT+TGG	-	chr4.2:12249139-12249158	None:intergenic	25.0%
!!!	TTGATTATTGGTTCTTCAAT+TGG	+	chr4.2:12251555-12251574	MS.gene53496:CDS	25.0%
!!!	TTTTTTTAGTTCCCCAAATA+AGG	+	chr4.2:12249536-12249555	MS.gene53496:intron	25.0%
	AACGTAATTGTTATAGCACA+AGG	+	chr4.2:12250445-12250464	MS.gene53496:intron	30.0%
	AAGGAGATATTATTCAAGTG+AGG	+	chr4.2:12251488-12251507	MS.gene53496:CDS	30.0%
	AATTGTATCCATTCTACAAG+AGG	-	chr4.2:12249968-12249987	None:intergenic	30.0%
	ATGGATACAATTACTCGAAA+AGG	+	chr4.2:12249976-12249995	MS.gene53496:intron	30.0%
	ATTATCTTCATAGTAATCGC+CGG	+	chr4.2:12250883-12250902	MS.gene53496:CDS	30.0%
	ATTGAGATTGTAGCATTCAT+AGG	-	chr4.2:12251357-12251376	None:intergenic	30.0%
	CAATTGGAATTTCAGCTTAT+TGG	+	chr4.2:12251571-12251590	MS.gene53496:CDS	30.0%
	CTCACAAAACAAATAGACTA+AGG	-	chr4.2:12249692-12249711	None:intergenic	30.0%
	GATCAGATTAAACATACTAG+TGG	+	chr4.2:12251876-12251895	MS.gene53496:CDS	30.0%
	GCTATTAACATATTCCTTCT+TGG	+	chr4.2:12251744-12251763	MS.gene53496:CDS	30.0%
	GCTTACTTAAGAGTAGAATT+GGG	+	chr4.2:12250586-12250605	MS.gene53496:CDS	30.0%
	TAAGTGGTACTCTACTAAAA+AGG	-	chr4.2:12250033-12250052	None:intergenic	30.0%
	TATCAACAACCTAGAGTAAT+CGG	-	chr4.2:12248807-12248826	None:intergenic	30.0%
	TGCTTACTTAAGAGTAGAAT+TGG	+	chr4.2:12250585-12250604	MS.gene53496:CDS	30.0%
!	CTTGTATACTATGTGCTATT+GGG	+	chr4.2:12251819-12251838	MS.gene53496:CDS	30.0%
!	GTACTTTTCTTTGCTTATGT+TGG	+	chr4.2:12250982-12251001	MS.gene53496:CDS	30.0%
!	GTTTTTCCCTCTTATATTTC+AGG	+	chr4.2:12250553-12250572	MS.gene53496:intron	30.0%
!	TATAAAACCCTCCTTATTTG+GGG	-	chr4.2:12249550-12249569	None:intergenic	30.0%
!	TGACATTTTCGTGATTGTTT+AGG	-	chr4.2:12250475-12250494	None:intergenic	30.0%
!	TTGTATACTATGTGCTATTG+GGG	+	chr4.2:12251820-12251839	MS.gene53496:CDS	30.0%
!	TTTAACTTTGGGTGCTTTAT+TGG	-	chr4.2:12249127-12249146	None:intergenic	30.0%
!!	AAACTTTTACCGATTACTCT+AGG	+	chr4.2:12248795-12248814	MS.gene53496:intron	30.0%
!!	TGTATTGAATGATGGTTTTG+GGG	-	chr4.2:12249515-12249534	None:intergenic	30.0%
	AAACTACGGAAAATCTGTCA+TGG	+	chr4.2:12248394-12248413	MS.gene53496:CDS	35.0%
	AACAATATAACTGCTCGTCT+TGG	+	chr4.2:12249916-12249935	MS.gene53496:intron	35.0%
	AAGAAATAGAGGACATTAGG+AGG	-	chr4.2:12249605-12249624	None:intergenic	35.0%
	ATAGATAAAGAAGGTAGCCA+AGG	-	chr4.2:12251723-12251742	None:intergenic	35.0%
	ATCCTCCTTGAGTATATCAT+AGG	+	chr4.2:12250640-12250659	MS.gene53496:CDS	35.0%
	ATTGAACCAACTAGACCAAT+TGG	-	chr4.2:12251063-12251082	None:intergenic	35.0%
	CAAGTGAGGTAACTACAAAA+GGG	+	chr4.2:12251502-12251521	MS.gene53496:CDS	35.0%
	CCAATAGCACATAGTATACA+AGG	-	chr4.2:12251821-12251840	None:intergenic	35.0%
	CCTTGTATACTATGTGCTAT+TGG	+	chr4.2:12251818-12251837	MS.gene53496:CDS	35.0%
	GACACACAATGAGATTTACT+TGG	-	chr4.2:12251530-12251549	None:intergenic	35.0%
	GATACAATTACTCGAAAAGG+TGG	+	chr4.2:12249979-12249998	MS.gene53496:intron	35.0%
	GCTAAGTACATTGTTTCACT+AGG	+	chr4.2:12251204-12251223	MS.gene53496:CDS	35.0%
	GGTACTCTACTAAAAAGGAT+TGG	-	chr4.2:12250028-12250047	None:intergenic	35.0%
	TCAAGTGAGGTAACTACAAA+AGG	+	chr4.2:12251501-12251520	MS.gene53496:CDS	35.0%
	TGGAAGAAATAGAGGACATT+AGG	-	chr4.2:12249608-12249627	None:intergenic	35.0%
	TTGCTTTCTTTACCGATCTT+GGG	+	chr4.2:12251397-12251416	MS.gene53496:CDS	35.0%
	TTGTTCCTCTGTGGTTTATA+GGG	+	chr4.2:12251631-12251650	MS.gene53496:CDS	35.0%
	TTTAAGAGTTGGGGAAACTA+CGG	+	chr4.2:12248380-12248399	MS.gene53496:CDS	35.0%
	TTTCCCTCTTATATTTCAGG+TGG	+	chr4.2:12250556-12250575	MS.gene53496:intron	35.0%
	TTTGTTCCTCTGTGGTTTAT+AGG	+	chr4.2:12251630-12251649	MS.gene53496:CDS	35.0%
!	TATCTTTTCACTTGTGGCAT+TGG	+	chr4.2:12251455-12251474	MS.gene53496:CDS	35.0%
!	TCTGGAATATTTGTGCTTAC+CGG	+	chr4.2:12248566-12248585	MS.gene53496:CDS	35.0%
!	TGTATTGTCGTTTGTCATCT+CGG	+	chr4.2:12248622-12248641	MS.gene53496:CDS	35.0%
!	TTCCCTCTTATATTTCAGGT+GGG	+	chr4.2:12250557-12250576	MS.gene53496:intron	35.0%
!	TTTAGTTCCCCAAATAAGGA+GGG	+	chr4.2:12249540-12249559	MS.gene53496:intron	35.0%
!!	CAATAAAAGGTTGGCTTAAG+TGG	-	chr4.2:12250049-12250068	None:intergenic	35.0%
!!	GGCTTATAGCTCTAATACTT+CGG	-	chr4.2:12250007-12250026	None:intergenic	35.0%
!!	GTGTGTCTTGTGTTGATTAT+TGG	+	chr4.2:12251543-12251562	MS.gene53496:CDS	35.0%
!!	TGCTTTGAAAGGAATGACAA+CGG	+	chr4.2:12251227-12251246	MS.gene53496:CDS	35.0%
!!	TTGTTATACAGAGTTTGCTG+TGG	+	chr4.2:12248670-12248689	MS.gene53496:CDS	35.0%
!!	TTTTGTTTGCGAGTTTGAAG+TGG	-	chr4.2:12249669-12249688	None:intergenic	35.0%
!!!	GTATTGAATGATGGTTTTGG+GGG	-	chr4.2:12249514-12249533	None:intergenic	35.0%
!!!	TATTGAATGATGGTTTTGGG+GGG	-	chr4.2:12249513-12249532	None:intergenic	35.0%
!!!	TCTTTGGCTTTTTGTTCCAA+TGG	+	chr4.2:12251662-12251681	MS.gene53496:CDS	35.0%
!!!	TTTTAGTTCCCCAAATAAGG+AGG	+	chr4.2:12249539-12249558	MS.gene53496:intron	35.0%
	AAACCACAGAGGAACAAAGA+TGG	-	chr4.2:12251628-12251647	None:intergenic	40.0%
	AAATAGAGGACATTAGGAGG+AGG	-	chr4.2:12249602-12249621	None:intergenic	40.0%
	AACAGTGAGAATGGATGGAT+TGG	+	chr4.2:12251597-12251616	MS.gene53496:CDS	40.0%
	AACTTAGGCTTCTTTGCCAT+TGG	-	chr4.2:12251681-12251700	None:intergenic	40.0%
	AATAGAGGACATTAGGAGGA+GGG	-	chr4.2:12249601-12249620	None:intergenic	40.0%
	ACAAAGTAGCAATCGACAAG+AGG	-	chr4.2:12251434-12251453	None:intergenic	40.0%
	ACAATCCACCTCTTGTAGAA+TGG	+	chr4.2:12249957-12249976	MS.gene53496:intron	40.0%
	AGATGAAGAAGACGTTGAAC+TGG	+	chr4.2:12248504-12248523	MS.gene53496:CDS	40.0%
	ATACTCAAGGAGGATGTTTC+CGG	-	chr4.2:12250635-12250654	None:intergenic	40.0%
	ATGACACACTTCAGCCATAT+TGG	+	chr4.2:12249806-12249825	MS.gene53496:intron	40.0%
	CAATTGAACAAGGTGGAACA+GGG	+	chr4.2:12251921-12251940	MS.gene53496:CDS	40.0%
	CAATTGGAATGTCTCTACCA+GGG	-	chr4.2:12251047-12251066	None:intergenic	40.0%
	CACAATATGAACCCTGACTA+TGG	+	chr4.2:12250745-12250764	MS.gene53496:CDS	40.0%
	CATAGTCAGGGTTCATATTG+TGG	-	chr4.2:12250747-12250766	None:intergenic	40.0%
	CGTATATAAGGTTTGCGGTT+TGG	+	chr4.2:12251784-12251803	MS.gene53496:CDS	40.0%
	CGTTGCCTATGATATACTCA+AGG	-	chr4.2:12250648-12250667	None:intergenic	40.0%
	CTTTGATGCAGTTTCAACCA+TGG	+	chr4.2:12251005-12251024	MS.gene53496:CDS	40.0%
	GACCCACCTGAAATATAAGA+GGG	-	chr4.2:12250562-12250581	None:intergenic	40.0%
	GCAAAGAAGCCTAAGTTATG+GGG	+	chr4.2:12251684-12251703	MS.gene53496:CDS	40.0%
	GCACTAAAAGCAACTGAGAA+AGG	-	chr4.2:12251168-12251187	None:intergenic	40.0%
	GGAGTAAGTGGAAGAAATAG+AGG	-	chr4.2:12249616-12249635	None:intergenic	40.0%
	GGCAAAGAAGCCTAAGTTAT+GGG	+	chr4.2:12251683-12251702	MS.gene53496:CDS	40.0%
	GGTTGAGAAGCAATTGAACA+AGG	+	chr4.2:12251911-12251930	MS.gene53496:CDS	40.0%
	GTTCGATAGAATCCCAAGAT+CGG	-	chr4.2:12251412-12251431	None:intergenic	40.0%
	GTTCTTAGTTTCTTCAGCCA+TGG	-	chr4.2:12251025-12251044	None:intergenic	40.0%
	TATTGGGCTAACAGTGAGAA+TGG	+	chr4.2:12251588-12251607	MS.gene53496:CDS	40.0%
	TCAAAAGAACGACTTCTTCC+CGG	+	chr4.2:12248349-12248368	MS.gene53496:CDS	40.0%
	TCATCAACAAGAGCAAACCA+AGG	-	chr4.2:12251321-12251340	None:intergenic	40.0%
	TGACCCACCTGAAATATAAG+AGG	-	chr4.2:12250563-12250582	None:intergenic	40.0%
	TGAGAAGCAATTGAACAAGG+TGG	+	chr4.2:12251914-12251933	MS.gene53496:CDS	40.0%
	TGCCTATGATATACTCAAGG+AGG	-	chr4.2:12250645-12250664	None:intergenic	40.0%
	TGGCAAAGAAGCCTAAGTTA+TGG	+	chr4.2:12251682-12251701	MS.gene53496:CDS	40.0%
	TGTATCCATTCTACAAGAGG+TGG	-	chr4.2:12249965-12249984	None:intergenic	40.0%
	TGTGGCTATGTAGTTGAAGA+TGG	-	chr4.2:12250845-12250864	None:intergenic	40.0%
	TTTGTTGCCTTTATAGCTGC+CGG	+	chr4.2:12250613-12250632	MS.gene53496:CDS	40.0%
!	AGTGAAGTCGTGGAAGTTTT+CGG	-	chr4.2:12250926-12250945	None:intergenic	40.0%
!	ATCAGGGTTTTTGTTGCAGA+GGG	-	chr4.2:12250707-12250726	None:intergenic	40.0%
!	CGAGTTTGAAGTGGAATAGA+GGG	-	chr4.2:12249660-12249679	None:intergenic	40.0%
!	GACATTCCAATTGGTCTAGT+TGG	+	chr4.2:12251054-12251073	MS.gene53496:CDS	40.0%
!	GAGTTTGAAGTGGAATAGAG+GGG	-	chr4.2:12249659-12249678	None:intergenic	40.0%
!	GCTCTTGTTGATGAAAGAAC+AGG	+	chr4.2:12251327-12251346	MS.gene53496:CDS	40.0%
!	GGGCATATCTGCTTTGTTAT+CGG	+	chr4.2:12248643-12248662	MS.gene53496:CDS	40.0%
!	GTATTGTCGTTTGTCATCTC+GGG	+	chr4.2:12248623-12248642	MS.gene53496:CDS	40.0%
!	GTTGCTTTCTTTACCGATCT+TGG	+	chr4.2:12251396-12251415	MS.gene53496:CDS	40.0%
!	GTTTCACTAGGTGCTTTGAA+AGG	+	chr4.2:12251216-12251235	MS.gene53496:CDS	40.0%
!	TAGTGCTGTTGGAATGGATT+GGG	+	chr4.2:12251182-12251201	MS.gene53496:CDS	40.0%
!	TTAGTGCTGTTGGAATGGAT+TGG	+	chr4.2:12251181-12251200	MS.gene53496:CDS	40.0%
!!	AAAGGTACTCCCCATAACTT+AGG	-	chr4.2:12251696-12251715	None:intergenic	40.0%
!!	ACATACTAGTGGTTCTGATC+AGG	+	chr4.2:12251887-12251906	MS.gene53496:CDS	40.0%
!!	AGAGTTTGCTGTGGAAATTC+CGG	+	chr4.2:12248679-12248698	MS.gene53496:CDS	40.0%
!!	AGGAATGACAACGGTTTTGT+TGG	+	chr4.2:12251236-12251255	MS.gene53496:CDS	40.0%
!!	GTAGAAAAAGTACCTGCAAC+CGG	-	chr4.2:12248701-12248720	None:intergenic	40.0%
!!	GTTTTGTTGGTTAGTGCTGT+TGG	+	chr4.2:12251249-12251268	MS.gene53496:CDS	40.0%
!!	TAAGTTATGGGGAGTACCTT+TGG	+	chr4.2:12251695-12251714	MS.gene53496:CDS	40.0%
!!	TGCTTTTAGTGCTGTTGGAA+TGG	+	chr4.2:12251176-12251195	MS.gene53496:CDS	40.0%
!!	TTTGGTGCTGTAAACTGCAA+GGG	-	chr4.2:12250815-12250834	None:intergenic	40.0%
!!!	TCAGTTGCTTTTAGTGCTGT+TGG	+	chr4.2:12251171-12251190	MS.gene53496:CDS	40.0%
	AAACGATCCTTCAACCGGTA+AGG	-	chr4.2:12248425-12248444	None:intergenic	45.0%
	AACTCTTAAACGACTCCTCC+GGG	-	chr4.2:12248370-12248389	None:intergenic	45.0%
	ACAAACGACAATACAACCGC+CGG	-	chr4.2:12248617-12248636	None:intergenic	45.0%
	ACACCATCTTTGTTCCTCTG+TGG	+	chr4.2:12251622-12251641	MS.gene53496:CDS	45.0%
	ATAGAGGACATTAGGAGGAG+GGG	-	chr4.2:12249600-12249619	None:intergenic	45.0%
	ATCTAGTGATCATGCAGAGC+TGG	+	chr4.2:12248454-12248473	MS.gene53496:CDS	45.0%
	ATTTGTGCTTACCGGACTTG+AGG	+	chr4.2:12248574-12248593	MS.gene53496:CDS	45.0%
	CAACTCTTAAACGACTCCTC+CGG	-	chr4.2:12248371-12248390	None:intergenic	45.0%
	CAATGGCAATAGGGTCAAGA+TGG	-	chr4.2:12250771-12250790	None:intergenic	45.0%
	CATAGCCACACTCTTACACA+TGG	+	chr4.2:12250855-12250874	MS.gene53496:CDS	45.0%
	CCAATTGGAATGTCTCTACC+AGG	-	chr4.2:12251048-12251067	None:intergenic	45.0%
	CCTGGTAGAGACATTCCAAT+TGG	+	chr4.2:12251045-12251064	MS.gene53496:CDS	45.0%
	CTGTCATGGAAACACCTTAC+CGG	+	chr4.2:12248408-12248427	MS.gene53496:CDS	45.0%
	CTTGCAGTTTACAGCACCAA+AGG	+	chr4.2:12250814-12250833	MS.gene53496:CDS	45.0%
	GAAGAAGACGTTGAACTGGT+GGG	+	chr4.2:12248508-12248527	MS.gene53496:CDS	45.0%
	GAGGAGTCGTTTAAGAGTTG+GGG	+	chr4.2:12248371-12248390	MS.gene53496:CDS	45.0%
	GATGTTTCCGGCAGCTATAA+AGG	-	chr4.2:12250623-12250642	None:intergenic	45.0%
	GCAACAAACGATCCTTCAAC+CGG	-	chr4.2:12248430-12248449	None:intergenic	45.0%
	GCAATTGAACAAGGTGGAAC+AGG	+	chr4.2:12251920-12251939	MS.gene53496:CDS	45.0%
	GCTGAAGAAACTAAGAACCC+TGG	+	chr4.2:12251027-12251046	MS.gene53496:CDS	45.0%
	GGAGGAGTCGTTTAAGAGTT+GGG	+	chr4.2:12248370-12248389	MS.gene53496:CDS	45.0%
	GTTGAAGATGGAAGAGCCTT+TGG	-	chr4.2:12250833-12250852	None:intergenic	45.0%
	TCAACAAGAGCAAACCAAGG+TGG	-	chr4.2:12251318-12251337	None:intergenic	45.0%
	TGAAGAAGACGTTGAACTGG+TGG	+	chr4.2:12248507-12248526	MS.gene53496:CDS	45.0%
	TGGAGATTAAGGCTAGGAGT+GGG	+	chr4.2:12248477-12248496	MS.gene53496:CDS	45.0%
	TTCCACGACTTCACTCCTTT+CGG	+	chr4.2:12250931-12250950	MS.gene53496:CDS	45.0%
!	ATAAGGTTTGCGGTTTGGAC+AGG	+	chr4.2:12251789-12251808	MS.gene53496:CDS	45.0%
!	CATCAGGGTTTTTGTTGCAG+AGG	-	chr4.2:12250708-12250727	None:intergenic	45.0%
!	GCGAGTTTGAAGTGGAATAG+AGG	-	chr4.2:12249661-12249680	None:intergenic	45.0%
!	GTGAAGTCGTGGAAGTTTTC+GGG	-	chr4.2:12250925-12250944	None:intergenic	45.0%
!	TAAGGTTTGCGGTTTGGACA+GGG	+	chr4.2:12251790-12251809	MS.gene53496:CDS	45.0%
!	TTATAGGGACTGGTGCTCTT+TGG	+	chr4.2:12251646-12251665	MS.gene53496:CDS	45.0%
!!	CTTTGGTGCTGTAAACTGCA+AGG	-	chr4.2:12250816-12250835	None:intergenic	45.0%
!!	GGGTTTGGCTTTGATTAGAC+CGG	-	chr4.2:12250905-12250924	None:intergenic	45.0%
!!	TACTAGTGGTTCTGATCAGG+AGG	+	chr4.2:12251890-12251909	MS.gene53496:CDS	45.0%
!!	TGGCATTGGCACTTCTAGTA+AGG	+	chr4.2:12251469-12251488	MS.gene53496:CDS	45.0%
!!!	AATGATGGTTTTGGGGGGTT+TGG	-	chr4.2:12249508-12249527	None:intergenic	45.0%
!!	AAAAAATTATAAAAAAAGTA+CGG	-	chr4.2:12249031-12249050	None:intergenic	5.0%
	AAAGAACGACTTCTTCCCGG+AGG	+	chr4.2:12248352-12248371	MS.gene53496:CDS	50.0%
	AAGGGCTGTGATGGCTACTA+GGG	-	chr4.2:12250797-12250816	None:intergenic	50.0%
	ACATTAGGAGGAGGGGCTTT+GGG	-	chr4.2:12249593-12249612	None:intergenic	50.0%
	ACCATACCATGAAGCCCGAA+AGG	-	chr4.2:12250949-12250968	None:intergenic	50.0%
	ACTAGGGCTGCAATGGCAAT+AGG	-	chr4.2:12250781-12250800	None:intergenic	50.0%
	AGCTGGTGGAGATTAAGGCT+AGG	+	chr4.2:12248471-12248490	MS.gene53496:CDS	50.0%
	CCAGTCCCTATAAACCACAG+AGG	-	chr4.2:12251639-12251658	None:intergenic	50.0%
	CCTCTGTGGTTTATAGGGAC+TGG	+	chr4.2:12251636-12251655	MS.gene53496:CDS	50.0%
	CGGAGGAGTCGTTTAAGAGT+TGG	+	chr4.2:12248369-12248388	MS.gene53496:CDS	50.0%
	CTAGGGCTGCAATGGCAATA+GGG	-	chr4.2:12250780-12250799	None:intergenic	50.0%
	GAAGGTAGCCAAGGAACCAA+AGG	-	chr4.2:12251714-12251733	None:intergenic	50.0%
	GACAGCCATGTGTAAGAGTG+TGG	-	chr4.2:12250863-12250882	None:intergenic	50.0%
	GGGCTAACAGTGAGAATGGA+TGG	+	chr4.2:12251592-12251611	MS.gene53496:CDS	50.0%
	GGTCAAGATGGCCATAGTCA+GGG	-	chr4.2:12250759-12250778	None:intergenic	50.0%
	GTAAACTGCAAGGGCTGTGA+TGG	-	chr4.2:12250806-12250825	None:intergenic	50.0%
	GTACTCACATGATGCCACCT+TGG	+	chr4.2:12251301-12251320	MS.gene53496:CDS	50.0%
	GTATATCATAGGCAACGCCG+CGG	+	chr4.2:12250651-12250670	MS.gene53496:CDS	50.0%
	GTGGAGATTAAGGCTAGGAG+TGG	+	chr4.2:12248476-12248495	MS.gene53496:CDS	50.0%
	TAGTGATCATGCAGAGCTGG+TGG	+	chr4.2:12248457-12248476	MS.gene53496:CDS	50.0%
	TCCACGACTTCACTCCTTTC+GGG	+	chr4.2:12250932-12250951	MS.gene53496:CDS	50.0%
	TCCTTTCGGGCTTCATGGTA+TGG	+	chr4.2:12250945-12250964	MS.gene53496:CDS	50.0%
	TGAACTGGTGGGACTTGATG+TGG	+	chr4.2:12248519-12248538	MS.gene53496:CDS	50.0%
	TGCAGAGCTGGTGGAGATTA+AGG	+	chr4.2:12248466-12248485	MS.gene53496:CDS	50.0%
	TGGTGGGACTTGATGTGGTT+CGG	+	chr4.2:12248524-12248543	MS.gene53496:CDS	50.0%
	TTCACTCCTTTCGGGCTTCA+TGG	+	chr4.2:12250940-12250959	MS.gene53496:CDS	50.0%
!	AGGGTTTTTGTTGCAGAGGG+TGG	-	chr4.2:12250704-12250723	None:intergenic	50.0%
!	GACTTGATGTGGTTCGGCAT+CGG	+	chr4.2:12248530-12248549	MS.gene53496:CDS	50.0%
!	GGAAACACCTTACCGGTTGA+AGG	+	chr4.2:12248415-12248434	MS.gene53496:CDS	50.0%
!!!	GTCGTGGAAGTTTTCGGGTT+TGG	-	chr4.2:12250920-12250939	None:intergenic	50.0%
	CAAGGGCTGTGATGGCTACT+AGG	-	chr4.2:12250798-12250817	None:intergenic	55.0%
	CATTAGGAGGAGGGGCTTTG+GGG	-	chr4.2:12249592-12249611	None:intergenic	55.0%
	GACATTAGGAGGAGGGGCTT+TGG	-	chr4.2:12249594-12249613	None:intergenic	55.0%
	GATGGCTACTAGGGCTGCAA+TGG	-	chr4.2:12250788-12250807	None:intergenic	55.0%
	GCTGTGGAAATTCCGGTTGC+AGG	+	chr4.2:12248686-12248705	MS.gene53496:CDS	55.0%
	GGGTCAAGATGGCCATAGTC+AGG	-	chr4.2:12250760-12250779	None:intergenic	55.0%
	TGCTTACCGGACTTGAGGCT+AGG	+	chr4.2:12248579-12248598	MS.gene53496:CDS	55.0%
	TTCCTGCCTAGCCTCAAGTC+CGG	-	chr4.2:12248588-12248607	None:intergenic	55.0%
!	ATTAGGAGGAGGGGCTTTGG+GGG	-	chr4.2:12249591-12249610	None:intergenic	55.0%
!!	ATCGGCGCCGTTATTGGTTC+TGG	+	chr4.2:12248548-12248567	MS.gene53496:CDS	55.0%
!!	GGGGAGTACCTTTGGTTCCT+TGG	+	chr4.2:12251703-12251722	MS.gene53496:CDS	55.0%
	CTTGAGGCTAGGCAGGAAGC+AGG	+	chr4.2:12248590-12248609	MS.gene53496:CDS	60.0%
	GCAGAGGGTGGCGAAATACG+AGG	-	chr4.2:12250692-12250711	None:intergenic	60.0%
	GCAGTTGGAGAGGTGGTCGA+TGG	+	chr4.2:12248296-12248315	MS.gene53496:CDS	60.0%
	GCCCGAAAGGAGTGAAGTCG+TGG	-	chr4.2:12250936-12250955	None:intergenic	60.0%
	GGTCCATGATCGAGCTACCG+CGG	-	chr4.2:12250671-12250690	None:intergenic	60.0%
	GTTGGAGAGGTGGTCGATGG+CGG	+	chr4.2:12248299-12248318	MS.gene53496:CDS	60.0%
	TACCGGACTTGAGGCTAGGC+AGG	+	chr4.2:12248583-12248602	MS.gene53496:CDS	60.0%
!!	TTCGGCATCGGCGCCGTTAT+TGG	+	chr4.2:12248542-12248561	MS.gene53496:CDS	60.0%
	ACGCCGCGGTAGCTCGATCA+TGG	+	chr4.2:12250665-12250684	MS.gene53496:CDS	65.0%
	GGCTAGGCAGGAAGCAGGTC+CGG	+	chr4.2:12248595-12248614	MS.gene53496:CDS	65.0%
	TAGGCAGGAAGCAGGTCCGG+CGG	+	chr4.2:12248598-12248617	MS.gene53496:CDS	65.0%
!	TCGATGGCGGCGGAGTACGT+AGG	+	chr4.2:12248312-12248331	MS.gene53496:CDS	65.0%
	GGCGGCGGAGTACGTAGGAG+AGG	+	chr4.2:12248317-12248336	MS.gene53496:CDS	70.0%
!	GGAGAGGTGGTCGATGGCGG+CGG	+	chr4.2:12248302-12248321	MS.gene53496:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	12248293	12251974	12248293	ID=MS.gene53496
chr4.2	mRNA	12248293	12251974	12248293	ID=MS.gene53496.t1;Parent=MS.gene53496
chr4.2	exon	12248293	12248707	12248293	ID=MS.gene53496.t1.exon1;Parent=MS.gene53496.t1
chr4.2	CDS	12248293	12248707	12248293	ID=cds.MS.gene53496.t1;Parent=MS.gene53496.t1
chr4.2	exon	12250575	12251974	12250575	ID=MS.gene53496.t1.exon2;Parent=MS.gene53496.t1
chr4.2	CDS	12250575	12251974	12250575	ID=cds.MS.gene53496.t1;Parent=MS.gene53496.t1

Gene Sequence

>MS.gene53496

ATGGCAGTTGGAGAGGTGGTCGATGGCGGCGGAGTACGTAGGAGAGGATGCACGTTTCAAAAGAACGACTTCTTCCCGGAGGAGTCGTTTAAGAGTTGGGGAAACTACGGAAAATCTGTCATGGAAACACCTTACCGGTTGAAGGATCGTTTGTTGCAACGATCTAGTGATCATGCAGAGCTGGTGGAGATTAAGGCTAGGAGTGGGAATGAGATGAAGAAGACGTTGAACTGGTGGGACTTGATGTGGTTCGGCATCGGCGCCGTTATTGGTTCTGGAATATTTGTGCTTACCGGACTTGAGGCTAGGCAGGAAGCAGGTCCGGCGGTTGTATTGTCGTTTGTCATCTCGGGCATATCTGCTTTGTTATCGGTATTTTGTTATACAGAGTTTGCTGTGGAAATTCCGGTTGCAGGTACTTTTTCTACTCTCACTATACTCTTTCTTATGCGTTATAATATTATGATTTGCATTAAATTTAATTTAGGGCATTGACAATAAGAAACTTTTACCGATTACTCTAGGTTGTTGATATAATAGCATCCTAAAAGTAAACAAAAAATTATAAGAGCATCACACCAACTTATAAACTTTTGAGTGTTACAAATAAAATTGAACTCTTGCGGATAATGATTTTCCCATAATTGAGAATTCAACTTTTTGAAACAATATTGACAATATTTTAGGAATGACACTGTTATTTATCAATCAATAAATGAATAATTAAATCGAATACCGTACTTTTTTTATAATTTTTTAATATTAATGTTTAGATAAATACTAAATTTCATCAACTCAACACTCACAACAAAGAAATAATAGATAGGTTCACCAATAAAGCACCCAAAGTTAAAACCAATAAAGCACCCAAATTTATAATTCCACAATATTAAAAGTGGCTATTTAACTTGATGTTTTCTTTTAAGGGATTTAACTTGATGTTAAATACTACCTTTTGAATTCTCTAAGATTCTAAGATTTTTACAACCTCGATGCATATTAATTAATTTAGACGAAAGATTTATTTAAGTCTATAAAAAAATGTGGCACTCAATTTTTATTTTTAAAGAAAAAAAAAACTATTGTGTCATGAGCTGATAGTTCTTTGTTTGACAAACATGAGTTGATAATTCTAAATATACATTTACTTTTTACAACTTTTGTTTTTAACAAATTATCCAACAAATTCTTAATAATTTCATATAAACTCTTCCAAACCCCCCAAAACCATCATTCAATACAATTTTTTTAGTTCCCCAAATAAGGAGGGTTTTATATTTTGAAGAAAAATAAGGCCCCCAAAGCCCCTCCTCCTAATGTCCTCTATTTCTTCCACTTACTCCTTTCTTTTTTTTCAAACTCTCCCCTCTATTCCACTTCAAACTCGCAAACAAAACCTTAGTCTATTTGTTTTGTGAGAAACCGATATTTTACTACCATAAGATTCACTAATGTTTAGTATAATTTTATTTTTTTATTATGGTATAATATCCTTTAATTTACATGAATACACATGACACACTTCAGCCATATTGGAATAAATAGTGTATTCCTTTAATTTATATTTATATTTATCAAATCACTAATATAGATGTTAGCAACAGAAGAGACATGTGTTTAAAAAACAATATAACTGCTCGTCTTGGATTGAGACAATACAAAAGACAATCCACCTCTTGTAGAATGGATACAATTACTCGAAAAGGTGGCACCGAAGTATTAGAGCTATAAGCCAATCCTTTTTAGTAGAGTACCACTTAAGCCAACCTTTTATTGATAATCTTTATATTCTTTTATGTTGATTTATCATTGATTTTTATAATTCAATGTAAGATTGAATATTTTTTTTGAATAACTGTAAGATTGAATATTGTTTTATCAATTATATCATTAAATACTTATTGCAGAGAAAAATAAAATAATTAAGACAATAAAATGTTAAGGTACGTAATGTAAAAAACTACAAATAATTTCTTCAAAACTAACAGTTTATTAGTTGTATTGATACGTGAATATAAACTTAAAACGACAACTAAAAAAGAACGGTGACACTTGAGAGTATATTCTTTTTGTGATTTAGGGAAAGATTATTAGCAGTTTTTTTTTTTAATATTTTTTTGAACAAGCAAAAATGAATTATATTAACACGCACAACGTAATTGTTATAGCACAAGGTGTGCCTAAACAATCACGAAAATGTCAAATGTCACGTAATTGTTATTAACAAGTTTTTTTTTTTAATATTGGTTTGTGTTTATATGTTTTTCCCTCTTATATTTCAGGTGGGTCATTTGCTTACTTAAGAGTAGAATTGGGAGATTTTGTTGCCTTTATAGCTGCCGGAAACATCCTCCTTGAGTATATCATAGGCAACGCCGCGGTAGCTCGATCATGGACCTCGTATTTCGCCACCCTCTGCAACAAAAACCCTGATGATTTTCGTATAATAGTCCACAATATGAACCCTGACTATGGCCATCTTGACCCTATTGCCATTGCAGCCCTAGTAGCCATCACAGCCCTTGCAGTTTACAGCACCAAAGGCTCTTCCATCTTCAACTACATAGCCACACTCTTACACATGGCTGTCATTATCTTCATAGTAATCGCCGGTCTAATCAAAGCCAAACCCGAAAACTTCCACGACTTCACTCCTTTCGGGCTTCATGGTATGGTCAATGCTTCAGCCGTACTTTTCTTTGCTTATGTTGGCTTTGATGCAGTTTCAACCATGGCTGAAGAAACTAAGAACCCTGGTAGAGACATTCCAATTGGTCTAGTTGGTTCAATGACGATTACAACAATAATCTATTGCTCACTAGCAGCAACGCTGTGTCTTATGCAGAACTACAAAGAACTAAATGTTGATGCTCCTTTCTCAGTTGCTTTTAGTGCTGTTGGAATGGATTGGGCTAAGTACATTGTTTCACTAGGTGCTTTGAAAGGAATGACAACGGTTTTGTTGGTTAGTGCTGTTGGTCAAGCTCGTTACTTAACTCACATTGCACGTACTCACATGATGCCACCTTGGTTTGCTCTTGTTGATGAAAGAACAGGAACGCCTATGAATGCTACAATCTCAATGCTTATCGCTACTGCTATCGTTGCTTTCTTTACCGATCTTGGGATTCTATCGAACCTCTTGTCGATTGCTACTTTGTTTATCTTTTCACTTGTGGCATTGGCACTTCTAGTAAGGAGATATTATTCAAGTGAGGTAACTACAAAAGGGAACCAAGTAAATCTCATTGTGTGTCTTGTGTTGATTATTGGTTCTTCAATTGGAATTTCAGCTTATTGGGCTAACAGTGAGAATGGATGGATTGGATACACCATCTTTGTTCCTCTGTGGTTTATAGGGACTGGTGCTCTTTGGCTTTTTGTTCCAATGGCAAAGAAGCCTAAGTTATGGGGAGTACCTTTGGTTCCTTGGCTACCTTCTTTATCTATTGCTATTAACATATTCCTTCTTGGATCTATTGATAAAAAATCGTATATAAGGTTTGCGGTTTGGACAGGGTTTCTCCTTGTATACTATGTGCTATTGGGGTTACATGCTTCATATGACACTGCAAAAGAGTTTGATCAGATTAAACATACTAGTGGTTCTGATCAGGAGGTTGAGAAGCAATTGAACAAGGTGGAACAGGGTGCTGTCGAACAAGACTCAAGTGTTGCTTAA

Protein sequence

>MS.gene53496.t1

MAVGEVVDGGGVRRRGCTFQKNDFFPEESFKSWGNYGKSVMETPYRLKDRLLQRSSDHAELVEIKARSGNEMKKTLNWWDLMWFGIGAVIGSGIFVLTGLEARQEAGPAVVLSFVISGISALLSVFCYTEFAVEIPVAGGSFAYLRVELGDFVAFIAAGNILLEYIIGNAAVARSWTSYFATLCNKNPDDFRIIVHNMNPDYGHLDPIAIAALVAITALAVYSTKGSSIFNYIATLLHMAVIIFIVIAGLIKAKPENFHDFTPFGLHGMVNASAVLFFAYVGFDAVSTMAEETKNPGRDIPIGLVGSMTITTIIYCSLAATLCLMQNYKELNVDAPFSVAFSAVGMDWAKYIVSLGALKGMTTVLLVSAVGQARYLTHIARTHMMPPWFALVDERTGTPMNATISMLIATAIVAFFTDLGILSNLLSIATLFIFSLVALALLVRRYYSSEVTTKGNQVNLIVCLVLIIGSSIGISAYWANSENGWIGYTIFVPLWFIGTGALWLFVPMAKKPKLWGVPLVPWLPSLSIAINIFLLGSIDKKSYIRFAVWTGFLLVYYVLLGLHASYDTAKEFDQIKHTSGSDQEVEKQLNKVEQGAVEQDSSVA