Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073450.t1 | XP_003594855.2 | 98.5 | 591 | 9 | 0 | 1 | 591 | 1 | 591 | 0.00E+00 | 1141.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073450.t1 | O64759 | 73.6 | 561 | 146 | 2 | 13 | 572 | 5 | 564 | 1.4e-235 | 817.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073450.t1 | G7INC3 | 98.5 | 591 | 9 | 0 | 1 | 591 | 1 | 591 | 0.0e+00 | 1141.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051253 | MS.gene073450 | 0.8187 | 1.71E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene073450 | -0.81648 | 5.41E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene073450 | 0.802494 | 5.46E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene073450 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene053555 | MS.gene073450 | 0.821582 | 3.74E-53 | -1.69E-46 |
| MS.gene053928 | MS.gene073450 | 0.803584 | 3.25E-49 | -1.69E-46 |
| MS.gene054010 | MS.gene073450 | 0.812361 | 4.41E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene073450 | 0.808599 | 2.86E-50 | -1.69E-46 |
| MS.gene054013 | MS.gene073450 | 0.804599 | 2.00E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene073450 | 0.841819 | 3.77E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene073450 | 0.822906 | 1.84E-53 | -1.69E-46 |
| MS.gene054556 | MS.gene073450 | 0.848384 | 6.34E-60 | -1.69E-46 |
| MS.gene055116 | MS.gene073450 | 0.804159 | 2.47E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene073450 | 0.800379 | 1.48E-48 | -1.69E-46 |
| MS.gene055604 | MS.gene073450 | 0.830289 | 3.19E-55 | -1.69E-46 |
| MS.gene056723 | MS.gene073450 | 0.836429 | 9.41E-57 | -1.69E-46 |
| MS.gene056902 | MS.gene073450 | 0.808226 | 3.44E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene073450 | 0.807391 | 5.17E-50 | -1.69E-46 |
| MS.gene058142 | MS.gene073450 | 0.820414 | 6.95E-53 | -1.69E-46 |
| MS.gene058949 | MS.gene073450 | 0.843338 | 1.49E-58 | -1.69E-46 |
| MS.gene059011 | MS.gene073450 | 0.832006 | 1.21E-55 | -1.69E-46 |
| MS.gene059420 | MS.gene073450 | 0.827794 | 1.29E-54 | -1.69E-46 |
| MS.gene059462 | MS.gene073450 | 0.804796 | 1.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene042480 | MS.gene073450 | PPI |
| MS.gene54521 | MS.gene073450 | PPI |
| MS.gene030903 | MS.gene073450 | PPI |
| MS.gene53496 | MS.gene073450 | PPI |
| MS.gene47928 | MS.gene073450 | PPI |
| MS.gene27968 | MS.gene073450 | PPI |
| MS.gene53497 | MS.gene073450 | PPI |
| MS.gene94408 | MS.gene073450 | PPI |
| MS.gene47930 | MS.gene073450 | PPI |
| MS.gene53499 | MS.gene073450 | PPI |
| MS.gene47931 | MS.gene073450 | PPI |
| MS.gene030905 | MS.gene073450 | PPI |
| MS.gene27967 | MS.gene073450 | PPI |
| MS.gene08148 | MS.gene073450 | PPI |
| MS.gene27966 | MS.gene073450 | PPI |
| MS.gene80913 | MS.gene073450 | PPI |
| MS.gene53498 | MS.gene073450 | PPI |
| MS.gene41790 | MS.gene073450 | PPI |
| MS.gene030907 | MS.gene073450 | PPI |
| MS.gene08144 | MS.gene073450 | PPI |
| MS.gene76957 | MS.gene073450 | PPI |
| MS.gene41792 | MS.gene073450 | PPI |
| MS.gene27969 | MS.gene073450 | PPI |
| MS.gene47929 | MS.gene073450 | PPI |
| MS.gene030906 | MS.gene073450 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073450.t1 | MTR_2g035430 | 98.477 | 591 | 9 | 0 | 1 | 591 | 1 | 591 | 0.0 | 1201 |
| MS.gene073450.t1 | MTR_8g461380 | 62.007 | 558 | 211 | 1 | 14 | 571 | 21 | 577 | 0.0 | 695 |
| MS.gene073450.t1 | MTR_8g089320 | 57.217 | 575 | 246 | 0 | 13 | 587 | 13 | 587 | 0.0 | 682 |
| MS.gene073450.t1 | MTR_1g030660 | 57.398 | 561 | 234 | 2 | 28 | 584 | 23 | 582 | 0.0 | 677 |
| MS.gene073450.t1 | MTR_8g089360 | 57.093 | 571 | 243 | 2 | 20 | 589 | 25 | 594 | 0.0 | 669 |
| MS.gene073450.t1 | MTR_8g089360 | 57.093 | 571 | 243 | 2 | 20 | 589 | 8 | 577 | 0.0 | 669 |
| MS.gene073450.t1 | MTR_8g089340 | 56.136 | 440 | 192 | 1 | 149 | 587 | 1 | 440 | 3.23e-166 | 481 |
| MS.gene073450.t1 | MTR_1g111740 | 41.536 | 573 | 317 | 5 | 28 | 585 | 6 | 575 | 3.16e-148 | 440 |
| MS.gene073450.t1 | MTR_4g099270 | 41.210 | 529 | 298 | 5 | 60 | 579 | 10 | 534 | 1.67e-126 | 383 |
| MS.gene073450.t1 | MTR_4g099270 | 42.559 | 383 | 216 | 2 | 34 | 412 | 5 | 387 | 1.18e-98 | 306 |
| MS.gene073450.t1 | MTR_4g099270 | 39.671 | 426 | 244 | 5 | 163 | 579 | 1 | 422 | 1.66e-93 | 294 |
| MS.gene073450.t1 | MTR_6g034535 | 59.471 | 227 | 84 | 2 | 361 | 580 | 89 | 314 | 3.39e-84 | 266 |
| MS.gene073450.t1 | MTR_6g034535 | 39.604 | 101 | 36 | 1 | 98 | 198 | 19 | 94 | 2.92e-13 | 71.6 |
| MS.gene073450.t1 | MTR_4g099270 | 41.722 | 302 | 172 | 2 | 34 | 331 | 5 | 306 | 1.83e-71 | 232 |
| MS.gene073450.t1 | MTR_6g027630 | 33.649 | 422 | 268 | 6 | 34 | 446 | 6 | 424 | 4.99e-69 | 234 |
| MS.gene073450.t1 | MTR_6g027630 | 33.649 | 422 | 268 | 6 | 34 | 446 | 6 | 424 | 1.40e-68 | 234 |
| MS.gene073450.t1 | MTR_6g027660 | 31.991 | 422 | 274 | 4 | 32 | 446 | 10 | 425 | 3.63e-67 | 230 |
| MS.gene073450.t1 | MTR_6g027680 | 31.323 | 431 | 282 | 4 | 24 | 446 | 3 | 427 | 8.12e-67 | 228 |
| MS.gene073450.t1 | MTR_7g086610 | 32.126 | 414 | 261 | 4 | 43 | 446 | 10 | 413 | 1.87e-66 | 228 |
| MS.gene073450.t1 | MTR_7g007850 | 31.021 | 519 | 342 | 10 | 65 | 572 | 42 | 555 | 2.14e-66 | 226 |
| MS.gene073450.t1 | MTR_7g086510 | 31.472 | 394 | 263 | 3 | 60 | 446 | 26 | 419 | 1.36e-64 | 223 |
| MS.gene073450.t1 | MTR_6g027650 | 31.429 | 420 | 273 | 4 | 34 | 446 | 6 | 417 | 2.98e-64 | 222 |
| MS.gene073450.t1 | MTR_7g007850 | 29.250 | 547 | 323 | 10 | 65 | 561 | 42 | 574 | 2.08e-63 | 219 |
| MS.gene073450.t1 | MTR_7g007850 | 33.249 | 397 | 256 | 7 | 65 | 455 | 42 | 435 | 1.31e-57 | 201 |
| MS.gene073450.t1 | MTR_7g007850 | 33.249 | 397 | 256 | 7 | 65 | 455 | 42 | 435 | 1.94e-57 | 202 |
| MS.gene073450.t1 | MTR_6g027630 | 33.432 | 338 | 216 | 4 | 117 | 446 | 3 | 339 | 4.61e-53 | 190 |
| MS.gene073450.t1 | MTR_8g089342 | 65.812 | 117 | 40 | 0 | 22 | 138 | 23 | 139 | 4.41e-49 | 167 |
| MS.gene073450.t1 | MTR_6g027670 | 32.278 | 158 | 107 | 0 | 289 | 446 | 1 | 158 | 1.53e-24 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073450.t1 | AT2G34960 | 73.619 | 561 | 146 | 2 | 13 | 572 | 5 | 564 | 0.0 | 862 |
| MS.gene073450.t1 | AT4G21120 | 61.498 | 574 | 219 | 1 | 1 | 574 | 1 | 572 | 0.0 | 737 |
| MS.gene073450.t1 | AT1G17120 | 61.525 | 564 | 216 | 1 | 8 | 571 | 17 | 579 | 0.0 | 717 |
| MS.gene073450.t1 | AT4G21120 | 59.716 | 422 | 170 | 0 | 153 | 574 | 1 | 422 | 0.0 | 531 |
| MS.gene073450.t1 | AT3G10600 | 42.984 | 563 | 287 | 6 | 27 | 567 | 11 | 561 | 5.79e-143 | 426 |
| MS.gene073450.t1 | AT5G04770 | 41.772 | 553 | 302 | 5 | 30 | 567 | 15 | 562 | 1.78e-142 | 425 |
| MS.gene073450.t1 | AT5G04770 | 43.411 | 387 | 209 | 2 | 30 | 406 | 15 | 401 | 2.82e-104 | 321 |
| MS.gene073450.t1 | AT3G10600 | 42.389 | 427 | 212 | 6 | 163 | 567 | 1 | 415 | 4.20e-104 | 322 |
| MS.gene073450.t1 | AT5G04770 | 41.429 | 420 | 226 | 5 | 163 | 567 | 1 | 415 | 2.68e-98 | 306 |
| MS.gene073450.t1 | AT3G03720 | 27.212 | 599 | 361 | 12 | 54 | 585 | 6 | 596 | 1.61e-68 | 233 |
| MS.gene073450.t1 | AT5G36940 | 32.668 | 401 | 259 | 3 | 55 | 448 | 16 | 412 | 2.11e-67 | 230 |
| MS.gene073450.t1 | AT1G05940 | 28.288 | 555 | 377 | 9 | 41 | 577 | 14 | 565 | 4.77e-64 | 220 |
| MS.gene073450.t1 | AT1G58030 | 30.340 | 412 | 276 | 3 | 42 | 446 | 14 | 421 | 1.29e-63 | 221 |
| MS.gene073450.t1 | AT1G05940 | 28.180 | 511 | 347 | 8 | 41 | 534 | 14 | 521 | 7.62e-57 | 200 |
| MS.gene073450.t1 | AT1G05940 | 28.918 | 453 | 306 | 6 | 41 | 478 | 14 | 465 | 3.87e-56 | 197 |
| MS.gene073450.t1 | AT1G05940 | 27.438 | 441 | 304 | 6 | 151 | 577 | 9 | 447 | 2.89e-45 | 166 |
Find 96 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGGGAATTTCAGCTTAT+TGG | 0.124964 | 2.4:+53612115 | MS.gene073450:CDS |
| TCAATTGGTGGGACCTAATT+TGG | 0.164490 | 2.4:+53610930 | MS.gene073450:CDS |
| AATGGGAATTTCAGCTTATT+GGG | 0.215067 | 2.4:+53612116 | MS.gene073450:CDS |
| GATGATATCCATTGCACTTT+TGG | 0.225184 | 2.4:+53612008 | MS.gene073450:CDS |
| TTTCAGCTTATTGGGGATTA+AGG | 0.251625 | 2.4:+53612124 | MS.gene073450:CDS |
| CACATCCAAGCTAGTGAAAA+AGG | 0.261858 | 2.4:-53611944 | None:intergenic |
| AGTCGCCTAATTCAATTCTT+AGG | 0.284521 | 2.4:-53611135 | None:intergenic |
| CCCTATAGCAGTTGGTGTTC+TGG | 0.294221 | 2.4:+53611321 | MS.gene073450:CDS |
| TTCAGAAAGTTGGAATGAAT+TGG | 0.299542 | 2.4:+53611725 | MS.gene073450:CDS |
| TCTACATTCAACTTATGATA+TGG | 0.303089 | 2.4:+53612386 | MS.gene073450:CDS |
| CTCATTGTTGCTTCTTCAAT+GGG | 0.303127 | 2.4:+53612099 | MS.gene073450:CDS |
| GCAAAATACGTAGTAGCTTT+TGG | 0.317716 | 2.4:+53611748 | MS.gene073450:CDS |
| GTAAGAAGGTATTATGTTAG+AGG | 0.322508 | 2.4:+53612030 | MS.gene073450:CDS |
| GCAATTCTTTACTTTGCATA+TGG | 0.325124 | 2.4:+53611523 | MS.gene073450:CDS |
| TTGATTCGAAGCGAATCTTT+TGG | 0.333880 | 2.4:-53611262 | None:intergenic |
| AGCAAAGGAAGCCAAAGTTT+TGG | 0.341150 | 2.4:+53612226 | MS.gene073450:CDS |
| GCCTACCTAAGAATTGAATT+AGG | 0.344020 | 2.4:+53611130 | MS.gene073450:CDS |
| CTCTTTCACAAACATGGTTA+AGG | 0.349691 | 2.4:+53610819 | MS.gene073450:CDS |
| CATCTCCAATAGCTTCTTTG+TGG | 0.353269 | 2.4:-53610737 | None:intergenic |
| TCTCATTGTTGCTTCTTCAA+TGG | 0.355395 | 2.4:+53612098 | MS.gene073450:CDS |
| ATCATATTCGTAATCATTGC+TGG | 0.358311 | 2.4:+53611427 | MS.gene073450:CDS |
| GACATACCAATCGGCTTGAT+TGG | 0.360474 | 2.4:+53611598 | MS.gene073450:CDS |
| GACCTAATTTGGTTTGGCTT+TGG | 0.371810 | 2.4:+53610941 | MS.gene073450:CDS |
| TCAGTTGCATTTCAGAAAGT+TGG | 0.376841 | 2.4:+53611715 | MS.gene073450:CDS |
| CACCAAAGCCAAACCAAATT+AGG | 0.385473 | 2.4:-53610943 | None:intergenic |
| ACCAGAACACCAACTGCTAT+AGG | 0.387682 | 2.4:-53611322 | None:intergenic |
| CTCAGTGGCATCGTCTGATC+TGG | 0.388850 | 2.4:-53610861 | None:intergenic |
| TCAGAAAGTTGGAATGAATT+GGG | 0.390454 | 2.4:+53611726 | MS.gene073450:CDS |
| TTTGGCTTTGGTGCAGTGAT+TGG | 0.393125 | 2.4:+53610953 | MS.gene073450:CDS |
| GCAGGAATCTTTGTGCTAAC+TGG | 0.395596 | 2.4:+53610977 | MS.gene073450:CDS |
| CACAGTCATCTATTGCTTGA+TGG | 0.401383 | 2.4:+53611636 | MS.gene073450:CDS |
| TTTGTTGCAATTGACAAAGA+TGG | 0.406903 | 2.4:-53612273 | None:intergenic |
| TGGTGGGACCTAATTTGGTT+TGG | 0.409509 | 2.4:+53610935 | MS.gene073450:CDS |
| GTCTTATCCTATGTAGCTTC+AGG | 0.410241 | 2.4:+53611034 | MS.gene073450:CDS |
| ACACTGTTACTGTTCCTGTT+TGG | 0.410710 | 2.4:+53612166 | MS.gene073450:CDS |
| AAGCCGATTGGTATGTCCTT+TGG | 0.412936 | 2.4:-53611592 | None:intergenic |
| ACAAACATGGTTAAGGTTCA+AGG | 0.416776 | 2.4:+53610826 | MS.gene073450:CDS |
| AAAGAGACTGCTAGGATTGA+AGG | 0.421585 | 2.4:+53612450 | MS.gene073450:CDS |
| AATACACCTTTAACTCCATA+AGG | 0.422612 | 2.4:-53611490 | None:intergenic |
| CAGCTTTGAAAGGATTCCTC+TGG | 0.424940 | 2.4:-53610779 | None:intergenic |
| ATGAACCACAAAGAAGCTAT+TGG | 0.431579 | 2.4:+53610732 | MS.gene073450:CDS |
| CTTTGAATGGCATCCACAAA+AGG | 0.433676 | 2.4:+53610698 | None:intergenic |
| GGGGAGTTCCACTTGTTCCA+TGG | 0.441381 | 2.4:+53612247 | MS.gene073450:CDS |
| TATTGGGGATTAAGGCCTAA+TGG | 0.442064 | 2.4:+53612132 | MS.gene073450:CDS |
| CCGATTGGTATGTCCTTTGG+CGG | 0.446653 | 2.4:-53611589 | None:intergenic |
| AGTGGAACTCCCCAAAACTT+TGG | 0.452180 | 2.4:-53612237 | None:intergenic |
| ATAAGAAGTGTAGCATTTAC+CGG | 0.457678 | 2.4:-53611898 | None:intergenic |
| CCAGAACACCAACTGCTATA+GGG | 0.459155 | 2.4:-53611321 | None:intergenic |
| TTAAGGCCTAATGGTTGGAT+TGG | 0.460463 | 2.4:+53612141 | MS.gene073450:CDS |
| GCATTCAAGAAGTATGTTAC+CGG | 0.465899 | 2.4:-53611176 | None:intergenic |
| GCTCTTGTACATCCGAAAAC+AGG | 0.481380 | 2.4:+53611871 | MS.gene073450:CDS |
| ATTCTTTACTTTGCATATGG+AGG | 0.482566 | 2.4:+53611526 | MS.gene073450:CDS |
| CTTCTGCTCTTTCACAAACA+TGG | 0.484220 | 2.4:+53610813 | MS.gene073450:CDS |
| GCTGTTGAAGTTCCAGCTGC+AGG | 0.487505 | 2.4:+53611097 | MS.gene073450:CDS |
| TGAAATTCCTGAAGCTACAT+AGG | 0.493757 | 2.4:-53611041 | None:intergenic |
| GTTCGCACATGATTCCTCCT+TGG | 0.495552 | 2.4:+53611845 | MS.gene073450:CDS |
| CAGAGGAATCCTTTCAAAGC+TGG | 0.496146 | 2.4:+53610780 | MS.gene073450:CDS |
| TTCTTTACTTTGCATATGGA+GGG | 0.502896 | 2.4:+53611527 | MS.gene073450:CDS |
| GCAAATGAACCACCTGCAGC+TGG | 0.513871 | 2.4:-53611109 | None:intergenic |
| AAAGCACACCATCTCAAAGA+TGG | 0.514487 | 2.4:+53611283 | MS.gene073450:CDS |
| GGGGATTAAGGCCTAATGGT+TGG | 0.518202 | 2.4:+53612136 | MS.gene073450:CDS |
| AAATACACAGATATAGATAC+AGG | 0.520624 | 2.4:+53611682 | MS.gene073450:CDS |
| TTTGGTGCAGTGATTGGTGC+AGG | 0.520646 | 2.4:+53610959 | MS.gene073450:CDS |
| GTGCAGCAGTATCAAGATCA+TGG | 0.523129 | 2.4:+53611209 | MS.gene073450:CDS |
| GCTTCTCAATTGGATTGCAT+CGG | 0.527593 | 2.4:+53611387 | MS.gene073450:CDS |
| GTTTGACAGTCTTGCAACCA+TGG | 0.540242 | 2.4:+53611549 | MS.gene073450:CDS |
| GTGTATCCAATCCAACCATT+AGG | 0.541391 | 2.4:-53612147 | None:intergenic |
| CACAAAGAAGCTATTGGAGA+TGG | 0.542271 | 2.4:+53610738 | MS.gene073450:CDS |
| TTACTAGACCCTATAGCAGT+TGG | 0.542343 | 2.4:+53611313 | MS.gene073450:CDS |
| TCATATTCGTAATCATTGCT+GGG | 0.546162 | 2.4:+53611428 | MS.gene073450:CDS |
| ATACTTCTTGAATGCGTACT+CGG | 0.547711 | 2.4:+53611184 | MS.gene073450:CDS |
| TTTGTTGCTTTCATAGCAGC+CGG | 0.549975 | 2.4:+53611157 | MS.gene073450:CDS |
| TGAGTCACAAAGAGACTGCT+AGG | 0.551629 | 2.4:+53612442 | MS.gene073450:CDS |
| CCGCCAAAGGACATACCAAT+CGG | 0.552434 | 2.4:+53611589 | MS.gene073450:CDS |
| ATAGTTGTTCCAGCTTTGAA+AGG | 0.559794 | 2.4:-53610789 | None:intergenic |
| AATTGTATCCATCTTTGAGA+TGG | 0.571253 | 2.4:-53611291 | None:intergenic |
| CAAGAAGCTCATAACCATGC+AGG | 0.571449 | 2.4:+53611001 | MS.gene073450:CDS |
| CCTTCTTACCAAAAGTGCAA+TGG | 0.572193 | 2.4:-53612016 | None:intergenic |
| ATTGACAAAGATGGCAACCA+TGG | 0.573355 | 2.4:-53612264 | None:intergenic |
| GATGGCAACCATGGAACAAG+TGG | 0.573911 | 2.4:-53612255 | None:intergenic |
| AATATGCACTTTGATAATGT+TGG | 0.578928 | 2.4:+53612344 | MS.gene073450:CDS |
| ATGGGAATTTCAGCTTATTG+GGG | 0.579215 | 2.4:+53612117 | MS.gene073450:CDS |
| AAGAGACTGCTAGGATTGAA+GGG | 0.588598 | 2.4:+53612451 | MS.gene073450:CDS |
| AATGGCATCCACAAAAGGCA+TGG | 0.591331 | 2.4:+53610703 | None:intergenic |
| GCCTAATTCAATTCTTAGGT+AGG | 0.592021 | 2.4:-53611131 | None:intergenic |
| AGACTATTGCTGCTCCTGCA+TGG | 0.592496 | 2.4:-53611015 | None:intergenic |
| CTTAAGCTCATGTGTCTCAG+TGG | 0.594867 | 2.4:-53610876 | None:intergenic |
| ATTGCATCGGCAGTCAACAT+AGG | 0.606531 | 2.4:+53611400 | MS.gene073450:CDS |
| ATTGAACCAATCAAGCCGAT+TGG | 0.614297 | 2.4:-53611604 | None:intergenic |
| TAAACAAGATTTCTTGCCAG+AGG | 0.615202 | 2.4:+53610763 | MS.gene073450:CDS |
| GTTGAAGTTCCAGCTGCAGG+TGG | 0.622133 | 2.4:+53611100 | MS.gene073450:CDS |
| TATGGCTCATCAACAAGAGA+AGG | 0.624424 | 2.4:+53612404 | MS.gene073450:CDS |
| ACATCCGAAAACAGGAACAC+CGG | 0.689157 | 2.4:+53611879 | MS.gene073450:CDS |
| TGTACAAGAGCGAACCAAGG+AGG | 0.725556 | 2.4:-53611859 | None:intergenic |
| GGATGTACAAGAGCGAACCA+AGG | 0.727262 | 2.4:-53611862 | None:intergenic |
| GCTGATCATTGCAATCATTG+CGG | 0.733326 | 2.4:-53611350 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAAAAAATGTCTCAAT+TGG | + | chr2.4:53610915-53610934 | MS.gene073450:CDS | 20.0% |
| !!! | TATGAAGCTTTTATAAGATT+TGG | + | chr2.4:53612321-53612340 | MS.gene073450:CDS | 20.0% |
| ! | AAATACACAGATATAGATAC+AGG | + | chr2.4:53611682-53611701 | MS.gene073450:CDS | 25.0% |
| ! | TCTACATTCAACTTATGATA+TGG | + | chr2.4:53612386-53612405 | MS.gene073450:CDS | 25.0% |
| ! | TTAACTCCATAAGGTAAAAA+AGG | - | chr2.4:53611484-53611503 | None:intergenic | 25.0% |
| !! | GCAACAAATATTTTTCTCAT+GGG | + | chr2.4:53612288-53612307 | MS.gene073450:CDS | 25.0% |
| !! | TGCAACAAATATTTTTCTCA+TGG | + | chr2.4:53612287-53612306 | MS.gene073450:CDS | 25.0% |
| !! | TTGGTTTACTATGTTTTCTT+TGG | + | chr2.4:53612363-53612382 | MS.gene073450:CDS | 25.0% |
| !! | TTTTTACCTTATGGAGTTAA+AGG | + | chr2.4:53611484-53611503 | MS.gene073450:CDS | 25.0% |
| !!! | AATATGCACTTTGATAATGT+TGG | + | chr2.4:53612344-53612363 | MS.gene073450:CDS | 25.0% |
| !!! | TTTGGTTTTTTTCAACTCTT+GGG | + | chr2.4:53612184-53612203 | MS.gene073450:CDS | 25.0% |
| AATACACCTTTAACTCCATA+AGG | - | chr2.4:53611493-53611512 | None:intergenic | 30.0% | |
| AATGGGAATTTCAGCTTATT+GGG | + | chr2.4:53612116-53612135 | MS.gene073450:CDS | 30.0% | |
| AGAGTTGAAAAAAACCAAAC+AGG | - | chr2.4:53612183-53612202 | None:intergenic | 30.0% | |
| ATCATATTCGTAATCATTGC+TGG | + | chr2.4:53611427-53611446 | MS.gene073450:CDS | 30.0% | |
| ATTCTTTACTTTGCATATGG+AGG | + | chr2.4:53611526-53611545 | MS.gene073450:CDS | 30.0% | |
| GAAAAAATGTCTCAATTGGT+GGG | + | chr2.4:53610919-53610938 | MS.gene073450:CDS | 30.0% | |
| GCAATTCTTTACTTTGCATA+TGG | + | chr2.4:53611523-53611542 | MS.gene073450:CDS | 30.0% | |
| TCAGAAAGTTGGAATGAATT+GGG | + | chr2.4:53611726-53611745 | MS.gene073450:CDS | 30.0% | |
| TCATATTCGTAATCATTGCT+GGG | + | chr2.4:53611428-53611447 | MS.gene073450:CDS | 30.0% | |
| TGAAAAAATGTCTCAATTGG+TGG | + | chr2.4:53610918-53610937 | MS.gene073450:CDS | 30.0% | |
| TTCAGAAAGTTGGAATGAAT+TGG | + | chr2.4:53611725-53611744 | MS.gene073450:CDS | 30.0% | |
| TTCTTTACTTTGCATATGGA+GGG | + | chr2.4:53611527-53611546 | MS.gene073450:CDS | 30.0% | |
| TTTGTTGCAATTGACAAAGA+TGG | - | chr2.4:53612276-53612295 | None:intergenic | 30.0% | |
| ! | AATCTTTTGGACGATTCAAA+AGG | - | chr2.4:53611252-53611271 | None:intergenic | 30.0% |
| ! | AATTGTATCCATCTTTGAGA+TGG | - | chr2.4:53611294-53611313 | None:intergenic | 30.0% |
| ! | ATAAGAAGTGTAGCATTTAC+CGG | - | chr2.4:53611901-53611920 | None:intergenic | 30.0% |
| ! | TATTTTTCTCATGGGATCTT+TGG | + | chr2.4:53612296-53612315 | MS.gene073450:CDS | 30.0% |
| !! | GTAAGAAGGTATTATGTTAG+AGG | + | chr2.4:53612030-53612049 | MS.gene073450:CDS | 30.0% |
| !!! | ATTTTTAGTTTCTTCAGCCA+TGG | - | chr2.4:53611569-53611588 | None:intergenic | 30.0% |
| !!! | CATATTTTTTGACTCAGCAA+AGG | + | chr2.4:53612211-53612230 | MS.gene073450:CDS | 30.0% |
| !!! | GTTTGGTTTTTTTCAACTCT+TGG | + | chr2.4:53612183-53612202 | MS.gene073450:CDS | 30.0% |
| !!! | TTGACACCTTTTTTACCTTA+TGG | + | chr2.4:53611475-53611494 | MS.gene073450:CDS | 30.0% |
| ACAAACATGGTTAAGGTTCA+AGG | + | chr2.4:53610826-53610845 | MS.gene073450:CDS | 35.0% | |
| AGAAACTAAAAATCCGCCAA+AGG | + | chr2.4:53611576-53611595 | MS.gene073450:CDS | 35.0% | |
| AGTCGCCTAATTCAATTCTT+AGG | - | chr2.4:53611138-53611157 | None:intergenic | 35.0% | |
| ATACTTCTTGAATGCGTACT+CGG | + | chr2.4:53611184-53611203 | MS.gene073450:CDS | 35.0% | |
| ATGAACCACAAAGAAGCTAT+TGG | + | chr2.4:53610732-53610751 | MS.gene073450:CDS | 35.0% | |
| ATGGGAATTTCAGCTTATTG+GGG | + | chr2.4:53612117-53612136 | MS.gene073450:CDS | 35.0% | |
| CAATGGGAATTTCAGCTTAT+TGG | + | chr2.4:53612115-53612134 | MS.gene073450:CDS | 35.0% | |
| CTCTTTCACAAACATGGTTA+AGG | + | chr2.4:53610819-53610838 | MS.gene073450:CDS | 35.0% | |
| GAAAAACTTCGCTTCTCAAT+TGG | + | chr2.4:53611377-53611396 | MS.gene073450:CDS | 35.0% | |
| GCCTAATTCAATTCTTAGGT+AGG | - | chr2.4:53611134-53611153 | None:intergenic | 35.0% | |
| GCCTACCTAAGAATTGAATT+AGG | + | chr2.4:53611130-53611149 | MS.gene073450:CDS | 35.0% | |
| TAAACAAGATTTCTTGCCAG+AGG | + | chr2.4:53610763-53610782 | MS.gene073450:CDS | 35.0% | |
| TCAGTTGCATTTCAGAAAGT+TGG | + | chr2.4:53611715-53611734 | MS.gene073450:CDS | 35.0% | |
| TGAAATTCCTGAAGCTACAT+AGG | - | chr2.4:53611044-53611063 | None:intergenic | 35.0% | |
| TTTCAGCTTATTGGGGATTA+AGG | + | chr2.4:53612124-53612143 | MS.gene073450:CDS | 35.0% | |
| ! | ATGACAACAGTGCTTTTAGT+AGG | + | chr2.4:53611784-53611803 | MS.gene073450:CDS | 35.0% |
| ! | CTCATTGTTGCTTCTTCAAT+GGG | + | chr2.4:53612099-53612118 | MS.gene073450:CDS | 35.0% |
| ! | GATGATATCCATTGCACTTT+TGG | + | chr2.4:53612008-53612027 | MS.gene073450:CDS | 35.0% |
| ! | GCAAAATACGTAGTAGCTTT+TGG | + | chr2.4:53611748-53611767 | MS.gene073450:CDS | 35.0% |
| ! | GCATTCAAGAAGTATGTTAC+CGG | - | chr2.4:53611179-53611198 | None:intergenic | 35.0% |
| ! | GTGCTTTTAGTAGGAAGATT+AGG | + | chr2.4:53611793-53611812 | MS.gene073450:CDS | 35.0% |
| ! | TCTCATTGTTGCTTCTTCAA+TGG | + | chr2.4:53612098-53612117 | MS.gene073450:CDS | 35.0% |
| !! | ATAGTTGTTCCAGCTTTGAA+AGG | - | chr2.4:53610792-53610811 | None:intergenic | 35.0% |
| !! | TTGATTCGAAGCGAATCTTT+TGG | - | chr2.4:53611265-53611284 | None:intergenic | 35.0% |
| AAAGAGACTGCTAGGATTGA+AGG | + | chr2.4:53612450-53612469 | MS.gene073450:CDS | 40.0% | |
| AAAGCACACCATCTCAAAGA+TGG | + | chr2.4:53611283-53611302 | MS.gene073450:CDS | 40.0% | |
| AAGAGACTGCTAGGATTGAA+GGG | + | chr2.4:53612451-53612470 | MS.gene073450:CDS | 40.0% | |
| ACACTGTTACTGTTCCTGTT+TGG | + | chr2.4:53612166-53612185 | MS.gene073450:CDS | 40.0% | |
| ATTGAACCAATCAAGCCGAT+TGG | - | chr2.4:53611607-53611626 | None:intergenic | 40.0% | |
| ATTGACAAAGATGGCAACCA+TGG | - | chr2.4:53612267-53612286 | None:intergenic | 40.0% | |
| CACAAAGAAGCTATTGGAGA+TGG | + | chr2.4:53610738-53610757 | MS.gene073450:CDS | 40.0% | |
| CACAGTCATCTATTGCTTGA+TGG | + | chr2.4:53611636-53611655 | MS.gene073450:CDS | 40.0% | |
| CACATCCAAGCTAGTGAAAA+AGG | - | chr2.4:53611947-53611966 | None:intergenic | 40.0% | |
| CACCAAAGCCAAACCAAATT+AGG | - | chr2.4:53610946-53610965 | None:intergenic | 40.0% | |
| CATCTCCAATAGCTTCTTTG+TGG | - | chr2.4:53610740-53610759 | None:intergenic | 40.0% | |
| CTTCTGCTCTTTCACAAACA+TGG | + | chr2.4:53610813-53610832 | MS.gene073450:CDS | 40.0% | |
| GCTGATCATTGCAATCATTG+CGG | - | chr2.4:53611353-53611372 | None:intergenic | 40.0% | |
| GCTTCTCAATTGGATTGCAT+CGG | + | chr2.4:53611387-53611406 | MS.gene073450:CDS | 40.0% | |
| GTCTTATCCTATGTAGCTTC+AGG | + | chr2.4:53611034-53611053 | MS.gene073450:CDS | 40.0% | |
| GTGTATCCAATCCAACCATT+AGG | - | chr2.4:53612150-53612169 | None:intergenic | 40.0% | |
| TATGGCTCATCAACAAGAGA+AGG | + | chr2.4:53612404-53612423 | MS.gene073450:CDS | 40.0% | |
| TATTGGGGATTAAGGCCTAA+TGG | + | chr2.4:53612132-53612151 | MS.gene073450:CDS | 40.0% | |
| TCAATTGGTGGGACCTAATT+TGG | + | chr2.4:53610930-53610949 | MS.gene073450:CDS | 40.0% | |
| TTAAGGCCTAATGGTTGGAT+TGG | + | chr2.4:53612141-53612160 | MS.gene073450:CDS | 40.0% | |
| TTACTAGACCCTATAGCAGT+TGG | + | chr2.4:53611313-53611332 | MS.gene073450:CDS | 40.0% | |
| ! | AGCAAAGGAAGCCAAAGTTT+TGG | + | chr2.4:53612226-53612245 | MS.gene073450:CDS | 40.0% |
| ! | ATTGTCATCCATGCCTTTTG+TGG | - | chr2.4:53610714-53610733 | None:intergenic | 40.0% |
| ! | CAAAGGAAGCCAAAGTTTTG+GGG | + | chr2.4:53612228-53612247 | MS.gene073450:CDS | 40.0% |
| ! | CCATTGCACTTTTGGTAAGA+AGG | + | chr2.4:53612016-53612035 | MS.gene073450:CDS | 40.0% |
| ! | CCTTCTTACCAAAAGTGCAA+TGG | - | chr2.4:53612019-53612038 | None:intergenic | 40.0% |
| ! | GCAAAGGAAGCCAAAGTTTT+GGG | + | chr2.4:53612227-53612246 | MS.gene073450:CDS | 40.0% |
| ! | TTTACCGGTGTTCCTGTTTT+CGG | - | chr2.4:53611886-53611905 | None:intergenic | 40.0% |
| !! | AGTAGCTTTTGGTGCATTGA+AGG | + | chr2.4:53611759-53611778 | MS.gene073450:CDS | 40.0% |
| !! | CATAGCCTTTTTCACTAGCT+TGG | + | chr2.4:53611939-53611958 | MS.gene073450:CDS | 40.0% |
| !! | GACCTAATTTGGTTTGGCTT+TGG | + | chr2.4:53610941-53610960 | MS.gene073450:CDS | 40.0% |
| !! | GTAGCTTTTGGTGCATTGAA+GGG | + | chr2.4:53611760-53611779 | MS.gene073450:CDS | 40.0% |
| !! | TTTGTTGCTTTCATAGCAGC+CGG | + | chr2.4:53611157-53611176 | MS.gene073450:CDS | 40.0% |
| AAGCCGATTGGTATGTCCTT+TGG | - | chr2.4:53611595-53611614 | None:intergenic | 45.0% | |
| ACATCCGAAAACAGGAACAC+CGG | + | chr2.4:53611879-53611898 | MS.gene073450:CDS | 45.0% | |
| ACCAGAACACCAACTGCTAT+AGG | - | chr2.4:53611325-53611344 | None:intergenic | 45.0% | |
| AGTGGAACTCCCCAAAACTT+TGG | - | chr2.4:53612240-53612259 | None:intergenic | 45.0% | |
| CAAGAAGCTCATAACCATGC+AGG | + | chr2.4:53611001-53611020 | MS.gene073450:CDS | 45.0% | |
| CAGAGGAATCCTTTCAAAGC+TGG | + | chr2.4:53610780-53610799 | MS.gene073450:CDS | 45.0% | |
| CCAGAACACCAACTGCTATA+GGG | - | chr2.4:53611324-53611343 | None:intergenic | 45.0% | |
| CTTAAGCTCATGTGTCTCAG+TGG | - | chr2.4:53610879-53610898 | None:intergenic | 45.0% | |
| GCAGGAATCTTTGTGCTAAC+TGG | + | chr2.4:53610977-53610996 | MS.gene073450:CDS | 45.0% | |
| GCTCTTGTACATCCGAAAAC+AGG | + | chr2.4:53611871-53611890 | MS.gene073450:CDS | 45.0% | |
| GTGCAGCAGTATCAAGATCA+TGG | + | chr2.4:53611209-53611228 | MS.gene073450:CDS | 45.0% | |
| GTTTGACAGTCTTGCAACCA+TGG | + | chr2.4:53611549-53611568 | MS.gene073450:CDS | 45.0% | |
| TGAGTCACAAAGAGACTGCT+AGG | + | chr2.4:53612442-53612461 | MS.gene073450:CDS | 45.0% | |
| TGGTGGGACCTAATTTGGTT+TGG | + | chr2.4:53610935-53610954 | MS.gene073450:CDS | 45.0% | |
| ! | ATTGCATCGGCAGTCAACAT+AGG | + | chr2.4:53611400-53611419 | MS.gene073450:CDS | 45.0% |
| !! | CAGCTTTGAAAGGATTCCTC+TGG | - | chr2.4:53610782-53610801 | None:intergenic | 45.0% |
| !! | GACATACCAATCGGCTTGAT+TGG | + | chr2.4:53611598-53611617 | MS.gene073450:CDS | 45.0% |
| !! | TTTGGCTTTGGTGCAGTGAT+TGG | + | chr2.4:53610953-53610972 | MS.gene073450:CDS | 45.0% |
| AGACTATTGCTGCTCCTGCA+TGG | - | chr2.4:53611018-53611037 | None:intergenic | 50.0% | |
| CCGATTGGTATGTCCTTTGG+CGG | - | chr2.4:53611592-53611611 | None:intergenic | 50.0% | |
| CCGCCAAAGGACATACCAAT+CGG | + | chr2.4:53611589-53611608 | MS.gene073450:CDS | 50.0% | |
| GATGGCAACCATGGAACAAG+TGG | - | chr2.4:53612258-53612277 | None:intergenic | 50.0% | |
| GGATGTACAAGAGCGAACCA+AGG | - | chr2.4:53611865-53611884 | None:intergenic | 50.0% | |
| GGGGATTAAGGCCTAATGGT+TGG | + | chr2.4:53612136-53612155 | MS.gene073450:CDS | 50.0% | |
| GTTCGCACATGATTCCTCCT+TGG | + | chr2.4:53611845-53611864 | MS.gene073450:CDS | 50.0% | |
| TGTACAAGAGCGAACCAAGG+AGG | - | chr2.4:53611862-53611881 | None:intergenic | 50.0% | |
| ! | CCCTATAGCAGTTGGTGTTC+TGG | + | chr2.4:53611321-53611340 | MS.gene073450:CDS | 50.0% |
| ! | TTTGGTGCAGTGATTGGTGC+AGG | + | chr2.4:53610959-53610978 | MS.gene073450:CDS | 50.0% |
| CTCAGTGGCATCGTCTGATC+TGG | - | chr2.4:53610864-53610883 | None:intergenic | 55.0% | |
| GCAAATGAACCACCTGCAGC+TGG | - | chr2.4:53611112-53611131 | None:intergenic | 55.0% | |
| GCTGTTGAAGTTCCAGCTGC+AGG | + | chr2.4:53611097-53611116 | MS.gene073450:CDS | 55.0% | |
| GGGGAGTTCCACTTGTTCCA+TGG | + | chr2.4:53612247-53612266 | MS.gene073450:CDS | 55.0% | |
| GTTGAAGTTCCAGCTGCAGG+TGG | + | chr2.4:53611100-53611119 | MS.gene073450:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 53610704 | 53612479 | 53610704 | ID=MS.gene073450 |
| chr2.4 | mRNA | 53610704 | 53612479 | 53610704 | ID=MS.gene073450.t1;Parent=MS.gene073450 |
| chr2.4 | exon | 53610704 | 53612479 | 53610704 | ID=MS.gene073450.t1.exon1;Parent=MS.gene073450.t1 |
| chr2.4 | CDS | 53610704 | 53612479 | 53610704 | ID=cds.MS.gene073450.t1;Parent=MS.gene073450.t1 |
| Gene Sequence |
| Protein sequence |
