Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58674.t1 | XP_003594855.2 | 98.1 | 585 | 11 | 0 | 1 | 585 | 7 | 591 | 0.00E+00 | 1125.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58674.t1 | O64759 | 73.6 | 561 | 146 | 2 | 7 | 566 | 5 | 564 | 5.1e-235 | 815.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58674.t1 | G7INC3 | 98.1 | 585 | 11 | 0 | 1 | 585 | 7 | 591 | 0.0e+00 | 1125.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene58674 | 0.80003 | 1.74E-48 | -1.69E-46 |
| MS.gene049346 | MS.gene58674 | 0.801494 | 8.77E-49 | -1.69E-46 |
| MS.gene050168 | MS.gene58674 | 0.836081 | 1.15E-56 | -1.69E-46 |
| MS.gene050226 | MS.gene58674 | 0.80998 | 1.45E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene58674 | 0.804375 | 2.23E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene58674 | 0.806899 | 6.57E-50 | -1.69E-46 |
| MS.gene050398 | MS.gene58674 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene050650 | MS.gene58674 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene051253 | MS.gene58674 | 0.806575 | 7.70E-50 | -1.69E-46 |
| MS.gene051525 | MS.gene58674 | 0.805076 | 1.59E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene58674 | -0.835178 | 1.95E-56 | -1.69E-46 |
| MS.gene052077 | MS.gene58674 | 0.809977 | 1.45E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene58674 | 0.802498 | 5.45E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene58674 | 0.82865 | 7.99E-55 | -1.69E-46 |
| MS.gene052255 | MS.gene58674 | 0.825148 | 5.49E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene58674 | 0.815708 | 8.05E-52 | -1.69E-46 |
| MS.gene053555 | MS.gene58674 | 0.813012 | 3.18E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene58674 | 0.806603 | 7.59E-50 | -1.69E-46 |
| MS.gene053928 | MS.gene58674 | 0.854545 | 1.15E-61 | -1.69E-46 |
| MS.gene054010 | MS.gene58674 | 0.82081 | 5.63E-53 | -1.69E-46 |
| MS.gene054011 | MS.gene58674 | 0.815474 | 9.08E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene58674 | 0.822389 | 2.43E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene58674 | 0.822389 | 2.43E-53 | -1.69E-46 |
| MS.gene054183 | MS.gene58674 | 0.843246 | 1.58E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene58674 | 0.815721 | 8.00E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene58674 | 0.823192 | 1.58E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene58674 | 0.810628 | 1.05E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene58674 | 0.83539 | 1.73E-56 | -1.69E-46 |
| MS.gene055604 | MS.gene58674 | 0.831011 | 2.13E-55 | -1.69E-46 |
| MS.gene056723 | MS.gene58674 | 0.841921 | 3.54E-58 | -1.69E-46 |
| MS.gene056902 | MS.gene58674 | 0.809123 | 2.21E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene58674 | 0.824081 | 9.79E-54 | -1.69E-46 |
| MS.gene057469 | MS.gene58674 | 0.818187 | 2.23E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene58674 | 0.82386 | 1.10E-53 | -1.69E-46 |
| MS.gene058949 | MS.gene58674 | 0.805132 | 1.55E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene58674 | 0.800861 | 1.18E-48 | -1.69E-46 |
| MS.gene05907 | MS.gene58674 | 0.806884 | 6.62E-50 | -1.69E-46 |
| MS.gene059247 | MS.gene58674 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene059420 | MS.gene58674 | 0.816452 | 5.49E-52 | -1.69E-46 |
| MS.gene059462 | MS.gene58674 | 0.803265 | 3.79E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene58674 | 0.831394 | 1.71E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene58674 | MS.gene80913 | PPI |
| MS.gene58674 | MS.gene47929 | PPI |
| MS.gene58674 | MS.gene030907 | PPI |
| MS.gene58674 | MS.gene08148 | PPI |
| MS.gene58674 | MS.gene41790 | PPI |
| MS.gene58674 | MS.gene08144 | PPI |
| MS.gene76957 | MS.gene58674 | PPI |
| MS.gene030903 | MS.gene58674 | PPI |
| MS.gene41792 | MS.gene58674 | PPI |
| MS.gene58674 | MS.gene53498 | PPI |
| MS.gene27969 | MS.gene58674 | PPI |
| MS.gene58674 | MS.gene53499 | PPI |
| MS.gene58674 | MS.gene27968 | PPI |
| MS.gene58674 | MS.gene94408 | PPI |
| MS.gene58674 | MS.gene030906 | PPI |
| MS.gene58674 | MS.gene47931 | PPI |
| MS.gene58674 | MS.gene47930 | PPI |
| MS.gene58674 | MS.gene54521 | PPI |
| MS.gene58674 | MS.gene27966 | PPI |
| MS.gene47928 | MS.gene58674 | PPI |
| MS.gene58674 | MS.gene53496 | PPI |
| MS.gene53497 | MS.gene58674 | PPI |
| MS.gene58674 | MS.gene042480 | PPI |
| MS.gene58674 | MS.gene27967 | PPI |
| MS.gene030905 | MS.gene58674 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58674.t1 | MTR_2g035430 | 98.120 | 585 | 11 | 0 | 1 | 585 | 7 | 591 | 0.0 | 1185 |
| MS.gene58674.t1 | MTR_8g461380 | 62.186 | 558 | 210 | 1 | 8 | 565 | 21 | 577 | 0.0 | 697 |
| MS.gene58674.t1 | MTR_8g089320 | 57.043 | 575 | 247 | 0 | 7 | 581 | 13 | 587 | 0.0 | 681 |
| MS.gene58674.t1 | MTR_1g030660 | 57.398 | 561 | 234 | 2 | 22 | 578 | 23 | 582 | 0.0 | 676 |
| MS.gene58674.t1 | MTR_8g089360 | 57.093 | 571 | 243 | 2 | 14 | 583 | 25 | 594 | 0.0 | 669 |
| MS.gene58674.t1 | MTR_8g089360 | 57.093 | 571 | 243 | 2 | 14 | 583 | 8 | 577 | 0.0 | 669 |
| MS.gene58674.t1 | MTR_8g089340 | 56.136 | 440 | 192 | 1 | 143 | 581 | 1 | 440 | 1.74e-166 | 482 |
| MS.gene58674.t1 | MTR_1g111740 | 40.830 | 578 | 314 | 5 | 22 | 579 | 6 | 575 | 8.24e-148 | 439 |
| MS.gene58674.t1 | MTR_4g099270 | 41.210 | 529 | 298 | 5 | 54 | 573 | 10 | 534 | 4.49e-126 | 382 |
| MS.gene58674.t1 | MTR_4g099270 | 42.559 | 383 | 216 | 2 | 28 | 406 | 5 | 387 | 8.51e-99 | 306 |
| MS.gene58674.t1 | MTR_4g099270 | 39.671 | 426 | 244 | 5 | 157 | 573 | 1 | 422 | 4.76e-93 | 293 |
| MS.gene58674.t1 | MTR_6g034535 | 59.912 | 227 | 83 | 2 | 355 | 574 | 89 | 314 | 1.31e-85 | 269 |
| MS.gene58674.t1 | MTR_6g034535 | 39.604 | 101 | 36 | 1 | 92 | 192 | 19 | 94 | 3.18e-13 | 71.2 |
| MS.gene58674.t1 | MTR_4g099270 | 41.722 | 302 | 172 | 2 | 28 | 325 | 5 | 306 | 1.96e-71 | 232 |
| MS.gene58674.t1 | MTR_6g027630 | 33.412 | 422 | 269 | 6 | 28 | 440 | 6 | 424 | 7.38e-69 | 233 |
| MS.gene58674.t1 | MTR_6g027630 | 33.412 | 422 | 269 | 6 | 28 | 440 | 6 | 424 | 1.81e-68 | 233 |
| MS.gene58674.t1 | MTR_6g027660 | 31.754 | 422 | 275 | 4 | 26 | 440 | 10 | 425 | 3.90e-67 | 230 |
| MS.gene58674.t1 | MTR_6g027680 | 31.090 | 431 | 283 | 4 | 18 | 440 | 3 | 427 | 1.36e-66 | 227 |
| MS.gene58674.t1 | MTR_7g086610 | 31.884 | 414 | 262 | 4 | 37 | 440 | 10 | 413 | 2.55e-66 | 228 |
| MS.gene58674.t1 | MTR_7g007850 | 30.829 | 519 | 343 | 10 | 59 | 566 | 42 | 555 | 6.21e-66 | 225 |
| MS.gene58674.t1 | MTR_7g086510 | 31.472 | 394 | 263 | 3 | 54 | 440 | 26 | 419 | 8.76e-65 | 224 |
| MS.gene58674.t1 | MTR_6g027650 | 31.190 | 420 | 274 | 4 | 28 | 440 | 6 | 417 | 3.60e-64 | 222 |
| MS.gene58674.t1 | MTR_7g007850 | 29.588 | 534 | 338 | 9 | 59 | 555 | 42 | 574 | 1.56e-63 | 219 |
| MS.gene58674.t1 | MTR_7g007850 | 33.249 | 397 | 256 | 7 | 59 | 449 | 42 | 435 | 9.23e-58 | 201 |
| MS.gene58674.t1 | MTR_7g007850 | 33.249 | 397 | 256 | 7 | 59 | 449 | 42 | 435 | 1.49e-57 | 202 |
| MS.gene58674.t1 | MTR_6g027630 | 33.136 | 338 | 217 | 4 | 111 | 440 | 3 | 339 | 4.53e-53 | 190 |
| MS.gene58674.t1 | MTR_8g089342 | 65.812 | 117 | 40 | 0 | 16 | 132 | 23 | 139 | 4.53e-49 | 167 |
| MS.gene58674.t1 | MTR_6g027670 | 31.646 | 158 | 108 | 0 | 283 | 440 | 1 | 158 | 2.36e-24 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58674.t1 | AT2G34960 | 73.619 | 561 | 146 | 2 | 7 | 566 | 5 | 564 | 0.0 | 859 |
| MS.gene58674.t1 | AT4G21120 | 61.837 | 566 | 216 | 0 | 3 | 568 | 7 | 572 | 0.0 | 735 |
| MS.gene58674.t1 | AT1G17120 | 62.057 | 564 | 213 | 1 | 2 | 565 | 17 | 579 | 0.0 | 722 |
| MS.gene58674.t1 | AT4G21120 | 59.953 | 422 | 169 | 0 | 147 | 568 | 1 | 422 | 0.0 | 530 |
| MS.gene58674.t1 | AT3G10600 | 42.629 | 563 | 289 | 6 | 21 | 561 | 11 | 561 | 1.96e-142 | 425 |
| MS.gene58674.t1 | AT5G04770 | 41.591 | 553 | 303 | 5 | 24 | 561 | 15 | 562 | 4.27e-142 | 424 |
| MS.gene58674.t1 | AT5G04770 | 43.152 | 387 | 210 | 2 | 24 | 400 | 15 | 401 | 2.94e-104 | 321 |
| MS.gene58674.t1 | AT3G10600 | 41.920 | 427 | 214 | 6 | 157 | 561 | 1 | 415 | 1.70e-103 | 320 |
| MS.gene58674.t1 | AT5G04770 | 41.190 | 420 | 227 | 5 | 157 | 561 | 1 | 415 | 5.45e-98 | 305 |
| MS.gene58674.t1 | AT3G03720 | 27.045 | 599 | 362 | 12 | 48 | 579 | 6 | 596 | 8.11e-69 | 234 |
| MS.gene58674.t1 | AT5G36940 | 32.918 | 401 | 258 | 3 | 49 | 442 | 16 | 412 | 7.88e-68 | 231 |
| MS.gene58674.t1 | AT1G05940 | 27.986 | 561 | 371 | 9 | 35 | 571 | 14 | 565 | 1.05e-63 | 219 |
| MS.gene58674.t1 | AT1G58030 | 30.751 | 413 | 273 | 5 | 36 | 440 | 14 | 421 | 1.46e-63 | 220 |
| MS.gene58674.t1 | AT1G05940 | 27.853 | 517 | 341 | 8 | 35 | 528 | 14 | 521 | 2.01e-56 | 199 |
| MS.gene58674.t1 | AT1G05940 | 28.918 | 453 | 306 | 6 | 35 | 472 | 14 | 465 | 2.39e-56 | 198 |
| MS.gene58674.t1 | AT1G05940 | 27.069 | 447 | 298 | 6 | 145 | 571 | 9 | 447 | 7.51e-45 | 165 |
Find 94 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGGGAATTTCAGCTTAT+TGG | 0.124964 | 2.3:+53548883 | MS.gene58674:CDS |
| TCAATTGGTGGGACCTAATT+TGG | 0.164490 | 2.3:+53547698 | MS.gene58674:CDS |
| AATGGGAATTTCAGCTTATT+GGG | 0.215067 | 2.3:+53548884 | MS.gene58674:CDS |
| GATGATATCCATTGCACTTT+TGG | 0.225184 | 2.3:+53548776 | MS.gene58674:CDS |
| CACATCCAAGCTAGTGAAAA+AGG | 0.261858 | 2.3:-53548712 | None:intergenic |
| AGTCGCCTAATTCAATTCTT+AGG | 0.284521 | 2.3:-53547903 | None:intergenic |
| ACACTGTTACTGTTCCTATT+TGG | 0.284631 | 2.3:+53548934 | MS.gene58674:CDS |
| GCAAAATATGTAGTAGCTTT+TGG | 0.291066 | 2.3:+53548516 | MS.gene58674:CDS |
| CCCTATAGCAGTTGGTGTTC+TGG | 0.294221 | 2.3:+53548089 | MS.gene58674:CDS |
| TTCAGAAAGTTGGAATGAAT+TGG | 0.299542 | 2.3:+53548493 | MS.gene58674:CDS |
| TCTACATTCAACTTATGATA+TGG | 0.303089 | 2.3:+53549154 | MS.gene58674:CDS |
| CTCATTGTTGCTTCTTCAAT+GGG | 0.303127 | 2.3:+53548867 | MS.gene58674:CDS |
| GTAAGAAGGTATTATGTTAG+AGG | 0.322508 | 2.3:+53548798 | MS.gene58674:CDS |
| GCAATTCTTTACTTTGCATA+TGG | 0.325124 | 2.3:+53548291 | MS.gene58674:CDS |
| TTGATTCGAAGCGAATCTTT+TGG | 0.333880 | 2.3:-53548030 | None:intergenic |
| GATATACCAATCGGCTTGAT+TGG | 0.334574 | 2.3:+53548366 | MS.gene58674:CDS |
| AGCAAAGGAAGCCAAAGTTT+TGG | 0.341150 | 2.3:+53548994 | MS.gene58674:CDS |
| GCCTACCTAAGAATTGAATT+AGG | 0.344020 | 2.3:+53547898 | MS.gene58674:CDS |
| ATCATATTCGTAATCATTGC+AGG | 0.347703 | 2.3:+53548195 | MS.gene58674:CDS |
| CTCTTTCACAAACATGGTTA+AGG | 0.349691 | 2.3:+53547587 | MS.gene58674:CDS |
| CATCTCCAATAGCTTCTTTG+TGG | 0.353269 | 2.3:-53547505 | None:intergenic |
| TCTCATTGTTGCTTCTTCAA+TGG | 0.355395 | 2.3:+53548866 | MS.gene58674:CDS |
| AACAGTTATATATTGCTTGA+TGG | 0.367904 | 2.3:+53548404 | MS.gene58674:CDS |
| GACCTAATTTGGTTTGGCTT+TGG | 0.371810 | 2.3:+53547709 | MS.gene58674:CDS |
| TCAGTTGCATTTCAGAAAGT+TGG | 0.376841 | 2.3:+53548483 | MS.gene58674:CDS |
| CACCAAAGCCAAACCAAATT+AGG | 0.385473 | 2.3:-53547711 | None:intergenic |
| ACCAGAACACCAACTGCTAT+AGG | 0.387682 | 2.3:-53548090 | None:intergenic |
| CTCAGTGGCATCGTCTGATC+TGG | 0.388850 | 2.3:-53547629 | None:intergenic |
| TCAGAAAGTTGGAATGAATT+GGG | 0.390454 | 2.3:+53548494 | MS.gene58674:CDS |
| TTTGGCTTTGGTGCAGTGAT+TGG | 0.393125 | 2.3:+53547721 | MS.gene58674:CDS |
| GCAGGAATCTTTGTGCTAAC+TGG | 0.395596 | 2.3:+53547745 | MS.gene58674:CDS |
| AAGCCGATTGGTATATCCTT+TGG | 0.407727 | 2.3:-53548360 | None:intergenic |
| ATCAGAAGTGTAGCATTTAT+CGG | 0.409131 | 2.3:-53548666 | None:intergenic |
| TGGTGGGACCTAATTTGGTT+TGG | 0.409509 | 2.3:+53547703 | MS.gene58674:CDS |
| GTCTTATCCTATGTAGCTTC+AGG | 0.410241 | 2.3:+53547802 | MS.gene58674:CDS |
| TTTGTTGCAATTGACAAAGA+TGG | 0.415372 | 2.3:-53549041 | None:intergenic |
| ACAAACATGGTTAAGGTTCA+AGG | 0.416776 | 2.3:+53547594 | MS.gene58674:CDS |
| AAAGAGACTGCTAGGATTGA+AGG | 0.421585 | 2.3:+53549218 | MS.gene58674:CDS |
| AATACACCTTTAACTCCATA+AGG | 0.422612 | 2.3:-53548258 | None:intergenic |
| CAGCTTTGAAAGGATTCCTC+TGG | 0.424940 | 2.3:-53547547 | None:intergenic |
| TATTGGGGATCGAGGCCTAA+TGG | 0.431391 | 2.3:+53548900 | MS.gene58674:CDS |
| ATGAACCACAAAGAAGCTAT+TGG | 0.431579 | 2.3:+53547500 | MS.gene58674:CDS |
| GCGCTTGTACATCCGAAAAC+AGG | 0.445402 | 2.3:+53548639 | MS.gene58674:CDS |
| AGTGGAACTCCCCAAAACTT+TGG | 0.452180 | 2.3:-53549005 | None:intergenic |
| CCAGAACACCAACTGCTATA+GGG | 0.459155 | 2.3:-53548089 | None:intergenic |
| GCATTCAAGAAGTATGTTAC+CGG | 0.465899 | 2.3:-53547944 | None:intergenic |
| GGGGAGTTCCACTTGTTCCA+TGG | 0.471354 | 2.3:+53549015 | MS.gene58674:CDS |
| CCGATTGGTATATCCTTTGG+TGG | 0.474482 | 2.3:-53548357 | None:intergenic |
| ATTCTTTACTTTGCATATGG+AGG | 0.482566 | 2.3:+53548294 | MS.gene58674:CDS |
| CTTCTGCTCTTTCACAAACA+TGG | 0.484220 | 2.3:+53547581 | MS.gene58674:CDS |
| GCTGTTGAAGTTCCAGCTGC+AGG | 0.487505 | 2.3:+53547865 | MS.gene58674:CDS |
| TCGAGGCCTAATGGTTGGAT+TGG | 0.491931 | 2.3:+53548909 | MS.gene58674:CDS |
| TGAAATTCCTGAAGCTACAT+AGG | 0.493757 | 2.3:-53547809 | None:intergenic |
| CAGAGGAATCCTTTCAAAGC+TGG | 0.496146 | 2.3:+53547548 | MS.gene58674:CDS |
| TTCTTTACTTTGCATATGGA+GGG | 0.502896 | 2.3:+53548295 | MS.gene58674:CDS |
| GCAAATGAACCACCTGCAGC+TGG | 0.513871 | 2.3:-53547877 | None:intergenic |
| AAAGCACACCATCTCAAAGA+TGG | 0.514487 | 2.3:+53548051 | MS.gene58674:CDS |
| AAATACACAGATATAGATAC+AGG | 0.520624 | 2.3:+53548450 | MS.gene58674:CDS |
| TTTGGTGCAGTGATTGGTGC+AGG | 0.520646 | 2.3:+53547727 | MS.gene58674:CDS |
| GTGCAGCAGTATCAAGATCA+TGG | 0.523129 | 2.3:+53547977 | MS.gene58674:CDS |
| GCTTCTCAATTGGATTGCAT+CGG | 0.527593 | 2.3:+53548155 | MS.gene58674:CDS |
| CCACCAAAGGATATACCAAT+CGG | 0.532745 | 2.3:+53548357 | MS.gene58674:CDS |
| GTTCACACATGATTCCTCCT+TGG | 0.536512 | 2.3:+53548613 | MS.gene58674:CDS |
| TTTAGTAGGAAGATTAGGAC+AGG | 0.539074 | 2.3:+53548566 | MS.gene58674:CDS |
| GTTTGACAGTCTTGCAACCA+TGG | 0.540242 | 2.3:+53548317 | MS.gene58674:CDS |
| GTGTATCCAATCCAACCATT+AGG | 0.541391 | 2.3:-53548915 | None:intergenic |
| CACAAAGAAGCTATTGGAGA+TGG | 0.542271 | 2.3:+53547506 | MS.gene58674:CDS |
| TTACTAGACCCTATAGCAGT+TGG | 0.542343 | 2.3:+53548081 | MS.gene58674:CDS |
| ATACTTCTTGAATGCGTACT+CGG | 0.547711 | 2.3:+53547952 | MS.gene58674:CDS |
| TTTGTTGCTTTCATAGCAGC+CGG | 0.549975 | 2.3:+53547925 | MS.gene58674:CDS |
| TGAGTCACAAAGAGACTGCT+AGG | 0.551629 | 2.3:+53549210 | MS.gene58674:CDS |
| GATGGTAACCATGGAACAAG+TGG | 0.553814 | 2.3:-53549023 | None:intergenic |
| GGGGATCGAGGCCTAATGGT+TGG | 0.558578 | 2.3:+53548904 | MS.gene58674:CDS |
| ATAGTTGTTCCAGCTTTGAA+AGG | 0.559794 | 2.3:-53547557 | None:intergenic |
| AATTGTATCCATCTTTGAGA+TGG | 0.571253 | 2.3:-53548059 | None:intergenic |
| CAAGAAGCTCATAACCATGC+AGG | 0.571449 | 2.3:+53547769 | MS.gene58674:CDS |
| CCTTCTTACCAAAAGTGCAA+TGG | 0.572193 | 2.3:-53548784 | None:intergenic |
| TTTCAGCTTATTGGGGATCG+AGG | 0.574349 | 2.3:+53548892 | MS.gene58674:CDS |
| AATATGCACTTTGATAATGT+TGG | 0.578928 | 2.3:+53549112 | MS.gene58674:CDS |
| ATGGGAATTTCAGCTTATTG+GGG | 0.579215 | 2.3:+53548885 | MS.gene58674:CDS |
| AAGAGACTGCTAGGATTGAA+GGG | 0.588598 | 2.3:+53549219 | MS.gene58674:CDS |
| GCCTAATTCAATTCTTAGGT+AGG | 0.592021 | 2.3:-53547899 | None:intergenic |
| AGACTATTGCTGCTCCTGCA+TGG | 0.592496 | 2.3:-53547783 | None:intergenic |
| CTTAAGCTCATGTGTCTCAG+TGG | 0.594867 | 2.3:-53547644 | None:intergenic |
| ATTGCATCGGCAGTCAACAT+AGG | 0.606531 | 2.3:+53548168 | MS.gene58674:CDS |
| TCATATTCGTAATCATTGCA+GGG | 0.613623 | 2.3:+53548196 | MS.gene58674:CDS |
| ATTGAACCAATCAAGCCGAT+TGG | 0.614297 | 2.3:-53548372 | None:intergenic |
| TAAACAAGATTTCTTGCCAG+AGG | 0.615202 | 2.3:+53547531 | MS.gene58674:CDS |
| ATTGACAAAGATGGTAACCA+TGG | 0.620125 | 2.3:-53549032 | None:intergenic |
| GTTGAAGTTCCAGCTGCAGG+TGG | 0.622133 | 2.3:+53547868 | MS.gene58674:CDS |
| TATGGCTCATCAACAAGAGA+AGG | 0.624424 | 2.3:+53549172 | MS.gene58674:CDS |
| GGATGTACAAGCGCGAACCA+AGG | 0.667921 | 2.3:-53548630 | None:intergenic |
| TGTACAAGCGCGAACCAAGG+AGG | 0.678479 | 2.3:-53548627 | None:intergenic |
| GCTGATCATTGCAATCATTG+CGG | 0.733326 | 2.3:-53548118 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAAAAAATGTCTCAAT+TGG | + | chr2.3:53547683-53547702 | MS.gene58674:CDS | 20.0% |
| !!! | GCAACAAATATTTTTCTTAT+GGG | + | chr2.3:53549056-53549075 | MS.gene58674:CDS | 20.0% |
| !!! | TATGAAGCTTTTATAAGATT+TGG | + | chr2.3:53549089-53549108 | MS.gene58674:CDS | 20.0% |
| !!! | TGCAACAAATATTTTTCTTA+TGG | + | chr2.3:53549055-53549074 | MS.gene58674:CDS | 20.0% |
| ! | AAATACACAGATATAGATAC+AGG | + | chr2.3:53548450-53548469 | MS.gene58674:CDS | 25.0% |
| ! | AACAGTTATATATTGCTTGA+TGG | + | chr2.3:53548404-53548423 | MS.gene58674:CDS | 25.0% |
| ! | AGAGTTGAAAAAAACCAAAT+AGG | - | chr2.3:53548951-53548970 | None:intergenic | 25.0% |
| ! | TCTACATTCAACTTATGATA+TGG | + | chr2.3:53549154-53549173 | MS.gene58674:CDS | 25.0% |
| ! | TTAACTCCATAAGGTAAAAA+AGG | - | chr2.3:53548252-53548271 | None:intergenic | 25.0% |
| !! | TATTTTTCTTATGGGATCTT+TGG | + | chr2.3:53549064-53549083 | MS.gene58674:CDS | 25.0% |
| !! | TTGGTTTACTATGTTTTCTT+TGG | + | chr2.3:53549131-53549150 | MS.gene58674:CDS | 25.0% |
| !! | TTTTTACCTTATGGAGTTAA+AGG | + | chr2.3:53548252-53548271 | MS.gene58674:CDS | 25.0% |
| !!! | AATATGCACTTTGATAATGT+TGG | + | chr2.3:53549112-53549131 | MS.gene58674:CDS | 25.0% |
| !!! | ATTTGGTTTTTTTCAACTCT+TGG | + | chr2.3:53548951-53548970 | MS.gene58674:CDS | 25.0% |
| !!! | TTTGGTTTTTTTCAACTCTT+GGG | + | chr2.3:53548952-53548971 | MS.gene58674:CDS | 25.0% |
| AATACACCTTTAACTCCATA+AGG | - | chr2.3:53548261-53548280 | None:intergenic | 30.0% | |
| AATGGGAATTTCAGCTTATT+GGG | + | chr2.3:53548884-53548903 | MS.gene58674:CDS | 30.0% | |
| AGAAACTAAAAATCCACCAA+AGG | + | chr2.3:53548344-53548363 | MS.gene58674:CDS | 30.0% | |
| ATCATATTCGTAATCATTGC+AGG | + | chr2.3:53548195-53548214 | MS.gene58674:CDS | 30.0% | |
| ATTCTTTACTTTGCATATGG+AGG | + | chr2.3:53548294-53548313 | MS.gene58674:CDS | 30.0% | |
| GAAAAAATGTCTCAATTGGT+GGG | + | chr2.3:53547687-53547706 | MS.gene58674:CDS | 30.0% | |
| GCAATTCTTTACTTTGCATA+TGG | + | chr2.3:53548291-53548310 | MS.gene58674:CDS | 30.0% | |
| TCAGAAAGTTGGAATGAATT+GGG | + | chr2.3:53548494-53548513 | MS.gene58674:CDS | 30.0% | |
| TCATATTCGTAATCATTGCA+GGG | + | chr2.3:53548196-53548215 | MS.gene58674:CDS | 30.0% | |
| TGAAAAAATGTCTCAATTGG+TGG | + | chr2.3:53547686-53547705 | MS.gene58674:CDS | 30.0% | |
| TTCAGAAAGTTGGAATGAAT+TGG | + | chr2.3:53548493-53548512 | MS.gene58674:CDS | 30.0% | |
| TTCTTTACTTTGCATATGGA+GGG | + | chr2.3:53548295-53548314 | MS.gene58674:CDS | 30.0% | |
| TTTGTTGCAATTGACAAAGA+TGG | - | chr2.3:53549044-53549063 | None:intergenic | 30.0% | |
| ! | AATTGTATCCATCTTTGAGA+TGG | - | chr2.3:53548062-53548081 | None:intergenic | 30.0% |
| ! | ATCAGAAGTGTAGCATTTAT+CGG | - | chr2.3:53548669-53548688 | None:intergenic | 30.0% |
| ! | GCAAAATATGTAGTAGCTTT+TGG | + | chr2.3:53548516-53548535 | MS.gene58674:CDS | 30.0% |
| !! | GTAAGAAGGTATTATGTTAG+AGG | + | chr2.3:53548798-53548817 | MS.gene58674:CDS | 30.0% |
| !!! | ATTTTTAGTTTCTTCAGCCA+TGG | - | chr2.3:53548337-53548356 | None:intergenic | 30.0% |
| !!! | CATATATTTTGACTCAGCAA+AGG | + | chr2.3:53548979-53548998 | MS.gene58674:CDS | 30.0% |
| !!! | TTGACACCTTTTTTACCTTA+TGG | + | chr2.3:53548243-53548262 | MS.gene58674:CDS | 30.0% |
| ACAAACATGGTTAAGGTTCA+AGG | + | chr2.3:53547594-53547613 | MS.gene58674:CDS | 35.0% | |
| ACACTGTTACTGTTCCTATT+TGG | + | chr2.3:53548934-53548953 | MS.gene58674:CDS | 35.0% | |
| AGTCGCCTAATTCAATTCTT+AGG | - | chr2.3:53547906-53547925 | None:intergenic | 35.0% | |
| ATACTTCTTGAATGCGTACT+CGG | + | chr2.3:53547952-53547971 | MS.gene58674:CDS | 35.0% | |
| ATGAACCACAAAGAAGCTAT+TGG | + | chr2.3:53547500-53547519 | MS.gene58674:CDS | 35.0% | |
| ATGGGAATTTCAGCTTATTG+GGG | + | chr2.3:53548885-53548904 | MS.gene58674:CDS | 35.0% | |
| ATTGACAAAGATGGTAACCA+TGG | - | chr2.3:53549035-53549054 | None:intergenic | 35.0% | |
| CAATGGGAATTTCAGCTTAT+TGG | + | chr2.3:53548883-53548902 | MS.gene58674:CDS | 35.0% | |
| CTCTTTCACAAACATGGTTA+AGG | + | chr2.3:53547587-53547606 | MS.gene58674:CDS | 35.0% | |
| GAAAAACTTCGCTTCTCAAT+TGG | + | chr2.3:53548145-53548164 | MS.gene58674:CDS | 35.0% | |
| GCCTAATTCAATTCTTAGGT+AGG | - | chr2.3:53547902-53547921 | None:intergenic | 35.0% | |
| GCCTACCTAAGAATTGAATT+AGG | + | chr2.3:53547898-53547917 | MS.gene58674:CDS | 35.0% | |
| TAAACAAGATTTCTTGCCAG+AGG | + | chr2.3:53547531-53547550 | MS.gene58674:CDS | 35.0% | |
| TCAGTTGCATTTCAGAAAGT+TGG | + | chr2.3:53548483-53548502 | MS.gene58674:CDS | 35.0% | |
| TGAAATTCCTGAAGCTACAT+AGG | - | chr2.3:53547812-53547831 | None:intergenic | 35.0% | |
| TTTAGTAGGAAGATTAGGAC+AGG | + | chr2.3:53548566-53548585 | MS.gene58674:CDS | 35.0% | |
| ! | AATCTTTTGGACGATTCAGA+AGG | - | chr2.3:53548020-53548039 | None:intergenic | 35.0% |
| ! | ATGACAACAGTGCTTTTAGT+AGG | + | chr2.3:53548552-53548571 | MS.gene58674:CDS | 35.0% |
| ! | CTCATTGTTGCTTCTTCAAT+GGG | + | chr2.3:53548867-53548886 | MS.gene58674:CDS | 35.0% |
| ! | GATGATATCCATTGCACTTT+TGG | + | chr2.3:53548776-53548795 | MS.gene58674:CDS | 35.0% |
| ! | GCATTCAAGAAGTATGTTAC+CGG | - | chr2.3:53547947-53547966 | None:intergenic | 35.0% |
| ! | GTGCTTTTAGTAGGAAGATT+AGG | + | chr2.3:53548561-53548580 | MS.gene58674:CDS | 35.0% |
| ! | TCTCATTGTTGCTTCTTCAA+TGG | + | chr2.3:53548866-53548885 | MS.gene58674:CDS | 35.0% |
| !! | ATAGTTGTTCCAGCTTTGAA+AGG | - | chr2.3:53547560-53547579 | None:intergenic | 35.0% |
| !! | TTGATTCGAAGCGAATCTTT+TGG | - | chr2.3:53548033-53548052 | None:intergenic | 35.0% |
| !! | TTTATCGGTGTTCCTGTTTT+CGG | - | chr2.3:53548654-53548673 | None:intergenic | 35.0% |
| AAAGAGACTGCTAGGATTGA+AGG | + | chr2.3:53549218-53549237 | MS.gene58674:CDS | 40.0% | |
| AAAGCACACCATCTCAAAGA+TGG | + | chr2.3:53548051-53548070 | MS.gene58674:CDS | 40.0% | |
| AAGAGACTGCTAGGATTGAA+GGG | + | chr2.3:53549219-53549238 | MS.gene58674:CDS | 40.0% | |
| AAGCCGATTGGTATATCCTT+TGG | - | chr2.3:53548363-53548382 | None:intergenic | 40.0% | |
| ATTGAACCAATCAAGCCGAT+TGG | - | chr2.3:53548375-53548394 | None:intergenic | 40.0% | |
| CACAAAGAAGCTATTGGAGA+TGG | + | chr2.3:53547506-53547525 | MS.gene58674:CDS | 40.0% | |
| CACATCCAAGCTAGTGAAAA+AGG | - | chr2.3:53548715-53548734 | None:intergenic | 40.0% | |
| CACCAAAGCCAAACCAAATT+AGG | - | chr2.3:53547714-53547733 | None:intergenic | 40.0% | |
| CATCTCCAATAGCTTCTTTG+TGG | - | chr2.3:53547508-53547527 | None:intergenic | 40.0% | |
| CCACCAAAGGATATACCAAT+CGG | + | chr2.3:53548357-53548376 | MS.gene58674:CDS | 40.0% | |
| CTTCTGCTCTTTCACAAACA+TGG | + | chr2.3:53547581-53547600 | MS.gene58674:CDS | 40.0% | |
| GCTGATCATTGCAATCATTG+CGG | - | chr2.3:53548121-53548140 | None:intergenic | 40.0% | |
| GCTTCTCAATTGGATTGCAT+CGG | + | chr2.3:53548155-53548174 | MS.gene58674:CDS | 40.0% | |
| GTCTTATCCTATGTAGCTTC+AGG | + | chr2.3:53547802-53547821 | MS.gene58674:CDS | 40.0% | |
| GTGTATCCAATCCAACCATT+AGG | - | chr2.3:53548918-53548937 | None:intergenic | 40.0% | |
| TATGGCTCATCAACAAGAGA+AGG | + | chr2.3:53549172-53549191 | MS.gene58674:CDS | 40.0% | |
| TCAATTGGTGGGACCTAATT+TGG | + | chr2.3:53547698-53547717 | MS.gene58674:CDS | 40.0% | |
| TTACTAGACCCTATAGCAGT+TGG | + | chr2.3:53548081-53548100 | MS.gene58674:CDS | 40.0% | |
| ! | AGCAAAGGAAGCCAAAGTTT+TGG | + | chr2.3:53548994-53549013 | MS.gene58674:CDS | 40.0% |
| ! | CAAAGGAAGCCAAAGTTTTG+GGG | + | chr2.3:53548996-53549015 | MS.gene58674:CDS | 40.0% |
| ! | CCATTGCACTTTTGGTAAGA+AGG | + | chr2.3:53548784-53548803 | MS.gene58674:CDS | 40.0% |
| ! | CCTTCTTACCAAAAGTGCAA+TGG | - | chr2.3:53548787-53548806 | None:intergenic | 40.0% |
| ! | GCAAAGGAAGCCAAAGTTTT+GGG | + | chr2.3:53548995-53549014 | MS.gene58674:CDS | 40.0% |
| !! | CATAGCCTTTTTCACTAGCT+TGG | + | chr2.3:53548707-53548726 | MS.gene58674:CDS | 40.0% |
| !! | GACCTAATTTGGTTTGGCTT+TGG | + | chr2.3:53547709-53547728 | MS.gene58674:CDS | 40.0% |
| !! | GATATACCAATCGGCTTGAT+TGG | + | chr2.3:53548366-53548385 | MS.gene58674:CDS | 40.0% |
| !! | GTAGCTTTTGGTGCATTGAA+AGG | + | chr2.3:53548528-53548547 | MS.gene58674:CDS | 40.0% |
| !! | TTTGTTGCTTTCATAGCAGC+CGG | + | chr2.3:53547925-53547944 | MS.gene58674:CDS | 40.0% |
| ACCAGAACACCAACTGCTAT+AGG | - | chr2.3:53548093-53548112 | None:intergenic | 45.0% | |
| AGTGGAACTCCCCAAAACTT+TGG | - | chr2.3:53549008-53549027 | None:intergenic | 45.0% | |
| CAAGAAGCTCATAACCATGC+AGG | + | chr2.3:53547769-53547788 | MS.gene58674:CDS | 45.0% | |
| CAGAGGAATCCTTTCAAAGC+TGG | + | chr2.3:53547548-53547567 | MS.gene58674:CDS | 45.0% | |
| CCAGAACACCAACTGCTATA+GGG | - | chr2.3:53548092-53548111 | None:intergenic | 45.0% | |
| CCGATTGGTATATCCTTTGG+TGG | - | chr2.3:53548360-53548379 | None:intergenic | 45.0% | |
| CTTAAGCTCATGTGTCTCAG+TGG | - | chr2.3:53547647-53547666 | None:intergenic | 45.0% | |
| GATGGTAACCATGGAACAAG+TGG | - | chr2.3:53549026-53549045 | None:intergenic | 45.0% | |
| GCAGGAATCTTTGTGCTAAC+TGG | + | chr2.3:53547745-53547764 | MS.gene58674:CDS | 45.0% | |
| GTGCAGCAGTATCAAGATCA+TGG | + | chr2.3:53547977-53547996 | MS.gene58674:CDS | 45.0% | |
| GTTCACACATGATTCCTCCT+TGG | + | chr2.3:53548613-53548632 | MS.gene58674:CDS | 45.0% | |
| GTTTGACAGTCTTGCAACCA+TGG | + | chr2.3:53548317-53548336 | MS.gene58674:CDS | 45.0% | |
| TGAGTCACAAAGAGACTGCT+AGG | + | chr2.3:53549210-53549229 | MS.gene58674:CDS | 45.0% | |
| TGGTGGGACCTAATTTGGTT+TGG | + | chr2.3:53547703-53547722 | MS.gene58674:CDS | 45.0% | |
| TTTCAGCTTATTGGGGATCG+AGG | + | chr2.3:53548892-53548911 | MS.gene58674:CDS | 45.0% | |
| ! | ATTGCATCGGCAGTCAACAT+AGG | + | chr2.3:53548168-53548187 | MS.gene58674:CDS | 45.0% |
| !! | CAGCTTTGAAAGGATTCCTC+TGG | - | chr2.3:53547550-53547569 | None:intergenic | 45.0% |
| !! | TTTGGCTTTGGTGCAGTGAT+TGG | + | chr2.3:53547721-53547740 | MS.gene58674:CDS | 45.0% |
| AGACTATTGCTGCTCCTGCA+TGG | - | chr2.3:53547786-53547805 | None:intergenic | 50.0% | |
| GCGCTTGTACATCCGAAAAC+AGG | + | chr2.3:53548639-53548658 | MS.gene58674:CDS | 50.0% | |
| TATTGGGGATCGAGGCCTAA+TGG | + | chr2.3:53548900-53548919 | MS.gene58674:CDS | 50.0% | |
| TCGAGGCCTAATGGTTGGAT+TGG | + | chr2.3:53548909-53548928 | MS.gene58674:CDS | 50.0% | |
| ! | CCCTATAGCAGTTGGTGTTC+TGG | + | chr2.3:53548089-53548108 | MS.gene58674:CDS | 50.0% |
| ! | TTTGGTGCAGTGATTGGTGC+AGG | + | chr2.3:53547727-53547746 | MS.gene58674:CDS | 50.0% |
| CTCAGTGGCATCGTCTGATC+TGG | - | chr2.3:53547632-53547651 | None:intergenic | 55.0% | |
| GCAAATGAACCACCTGCAGC+TGG | - | chr2.3:53547880-53547899 | None:intergenic | 55.0% | |
| GCTGTTGAAGTTCCAGCTGC+AGG | + | chr2.3:53547865-53547884 | MS.gene58674:CDS | 55.0% | |
| GGATGTACAAGCGCGAACCA+AGG | - | chr2.3:53548633-53548652 | None:intergenic | 55.0% | |
| GGGGAGTTCCACTTGTTCCA+TGG | + | chr2.3:53549015-53549034 | MS.gene58674:CDS | 55.0% | |
| GTTGAAGTTCCAGCTGCAGG+TGG | + | chr2.3:53547868-53547887 | MS.gene58674:CDS | 55.0% | |
| TGTACAAGCGCGAACCAAGG+AGG | - | chr2.3:53548630-53548649 | None:intergenic | 55.0% | |
| GGGGATCGAGGCCTAATGGT+TGG | + | chr2.3:53548904-53548923 | MS.gene58674:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 53547490 | 53549247 | 53547490 | ID=MS.gene58674 |
| chr2.3 | mRNA | 53547490 | 53549247 | 53547490 | ID=MS.gene58674.t1;Parent=MS.gene58674 |
| chr2.3 | exon | 53547490 | 53549247 | 53547490 | ID=MS.gene58674.t1.exon1;Parent=MS.gene58674.t1 |
| chr2.3 | CDS | 53547490 | 53549247 | 53547490 | ID=cds.MS.gene58674.t1;Parent=MS.gene58674.t1 |
| Gene Sequence |
| Protein sequence |
