Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007811.t1 | RHN40813.1 | 96.1 | 513 | 18 | 1 | 1 | 513 | 79 | 589 | 1.30E-273 | 951.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007811.t1 | Q9SHH0 | 72.7 | 498 | 136 | 0 | 1 | 498 | 81 | 578 | 7.3e-209 | 728.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007811.t1 | A0A396GJV2 | 96.1 | 513 | 18 | 1 | 1 | 513 | 79 | 589 | 9.3e-274 | 951.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene007811 | MS.gene064542 | PPI |
| MS.gene007811 | MS.gene47931 | PPI |
| MS.gene007811 | MS.gene029217 | PPI |
| MS.gene007811 | MS.gene53498 | PPI |
| MS.gene007811 | MS.gene53499 | PPI |
| MS.gene007811 | MS.gene27968 | PPI |
| MS.gene007811 | MS.gene27969 | PPI |
| MS.gene007811 | MS.gene029219 | PPI |
| MS.gene007811 | MS.gene90825 | PPI |
| MS.gene007811 | MS.gene47930 | PPI |
| MS.gene007811 | MS.gene73119 | PPI |
| MS.gene007811 | MS.gene27966 | PPI |
| MS.gene007811 | MS.gene97346 | PPI |
| MS.gene007811 | MS.gene93135 | PPI |
| MS.gene007811 | MS.gene029093 | PPI |
| MS.gene007811 | MS.gene030903 | PPI |
| MS.gene007811 | MS.gene53496 | PPI |
| MS.gene007811 | MS.gene53497 | PPI |
| MS.gene007811 | MS.gene030907 | PPI |
| MS.gene007811 | MS.gene23912 | PPI |
| MS.gene007811 | MS.gene27967 | PPI |
| MS.gene007811 | MS.gene066439 | PPI |
| MS.gene007811 | MS.gene037356 | PPI |
| MS.gene007811 | MS.gene47928 | PPI |
| MS.gene007811 | MS.gene47929 | PPI |
| MS.gene007811 | MS.gene035805 | PPI |
| MS.gene007811 | MS.gene030906 | PPI |
| MS.gene007811 | MS.gene030905 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007811.t1 | MTR_8g461380 | 95.906 | 513 | 19 | 1 | 1 | 513 | 79 | 589 | 0.0 | 989 |
| MS.gene007811.t1 | MTR_2g035430 | 61.996 | 521 | 187 | 4 | 1 | 512 | 72 | 590 | 0.0 | 651 |
| MS.gene007811.t1 | MTR_8g089320 | 50.680 | 515 | 250 | 2 | 1 | 511 | 72 | 586 | 0.0 | 532 |
| MS.gene007811.t1 | MTR_8g089360 | 51.677 | 507 | 241 | 3 | 1 | 506 | 77 | 580 | 0.0 | 522 |
| MS.gene007811.t1 | MTR_8g089360 | 51.677 | 507 | 241 | 3 | 1 | 506 | 60 | 563 | 0.0 | 522 |
| MS.gene007811.t1 | MTR_1g030660 | 48.837 | 516 | 259 | 3 | 1 | 515 | 67 | 578 | 3.95e-180 | 519 |
| MS.gene007811.t1 | MTR_6g034535 | 86.235 | 247 | 30 | 1 | 273 | 515 | 68 | 314 | 6.98e-152 | 436 |
| MS.gene007811.t1 | MTR_6g034535 | 67.708 | 96 | 6 | 1 | 27 | 122 | 19 | 89 | 6.20e-29 | 117 |
| MS.gene007811.t1 | MTR_8g089340 | 48.364 | 428 | 219 | 2 | 78 | 503 | 1 | 428 | 2.37e-135 | 400 |
| MS.gene007811.t1 | MTR_1g111740 | 40.607 | 527 | 283 | 7 | 1 | 506 | 50 | 567 | 5.10e-126 | 380 |
| MS.gene007811.t1 | MTR_4g099270 | 38.933 | 506 | 300 | 5 | 1 | 498 | 22 | 526 | 4.98e-106 | 328 |
| MS.gene007811.t1 | MTR_4g099270 | 42.433 | 337 | 190 | 2 | 1 | 333 | 43 | 379 | 3.50e-83 | 263 |
| MS.gene007811.t1 | MTR_4g099270 | 36.145 | 415 | 256 | 5 | 92 | 498 | 1 | 414 | 1.56e-77 | 250 |
| MS.gene007811.t1 | MTR_7g007850 | 31.369 | 526 | 333 | 10 | 1 | 508 | 49 | 564 | 5.69e-64 | 218 |
| MS.gene007811.t1 | MTR_6g027680 | 31.675 | 382 | 254 | 2 | 1 | 375 | 46 | 427 | 6.21e-61 | 210 |
| MS.gene007811.t1 | MTR_7g007850 | 30.109 | 548 | 341 | 11 | 1 | 508 | 49 | 594 | 6.92e-61 | 210 |
| MS.gene007811.t1 | MTR_4g099270 | 42.045 | 264 | 149 | 3 | 1 | 260 | 43 | 306 | 1.02e-59 | 199 |
| MS.gene007811.t1 | MTR_6g027630 | 31.937 | 382 | 253 | 3 | 1 | 375 | 43 | 424 | 1.94e-59 | 206 |
| MS.gene007811.t1 | MTR_6g027630 | 31.937 | 382 | 253 | 3 | 1 | 375 | 43 | 424 | 3.58e-59 | 206 |
| MS.gene007811.t1 | MTR_7g086510 | 31.152 | 382 | 256 | 3 | 1 | 375 | 38 | 419 | 6.99e-58 | 203 |
| MS.gene007811.t1 | MTR_6g027660 | 31.414 | 382 | 255 | 2 | 1 | 375 | 44 | 425 | 2.28e-57 | 201 |
| MS.gene007811.t1 | MTR_6g027650 | 30.366 | 382 | 259 | 3 | 1 | 375 | 36 | 417 | 2.61e-56 | 199 |
| MS.gene007811.t1 | MTR_7g007850 | 32.134 | 417 | 272 | 6 | 1 | 408 | 49 | 463 | 6.31e-56 | 195 |
| MS.gene007811.t1 | MTR_7g086610 | 27.069 | 580 | 354 | 8 | 1 | 511 | 32 | 611 | 6.48e-56 | 197 |
| MS.gene007811.t1 | MTR_7g007850 | 32.134 | 417 | 272 | 6 | 1 | 408 | 49 | 463 | 2.16e-55 | 194 |
| MS.gene007811.t1 | MTR_6g027630 | 31.157 | 337 | 225 | 3 | 46 | 375 | 3 | 339 | 4.95e-48 | 174 |
| MS.gene007811.t1 | MTR_6g027670 | 32.911 | 158 | 106 | 0 | 218 | 375 | 1 | 158 | 4.44e-25 | 105 |
| MS.gene007811.t1 | MTR_8g089342 | 64.179 | 67 | 24 | 0 | 1 | 67 | 73 | 139 | 2.89e-22 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007811.t1 | AT1G17120 | 71.569 | 510 | 144 | 1 | 1 | 509 | 81 | 590 | 0.0 | 733 |
| MS.gene007811.t1 | AT2G34960 | 62.800 | 500 | 183 | 3 | 1 | 498 | 64 | 562 | 0.0 | 646 |
| MS.gene007811.t1 | AT4G21120 | 52.148 | 512 | 244 | 1 | 1 | 511 | 70 | 581 | 0.0 | 548 |
| MS.gene007811.t1 | AT4G21120 | 49.420 | 431 | 217 | 1 | 82 | 511 | 1 | 431 | 4.20e-148 | 432 |
| MS.gene007811.t1 | AT5G04770 | 41.879 | 511 | 280 | 5 | 1 | 497 | 57 | 564 | 9.63e-124 | 374 |
| MS.gene007811.t1 | AT3G10600 | 40.832 | 529 | 288 | 8 | 1 | 511 | 56 | 577 | 3.02e-122 | 370 |
| MS.gene007811.t1 | AT5G04770 | 45.322 | 342 | 177 | 2 | 1 | 332 | 57 | 398 | 1.91e-91 | 285 |
| MS.gene007811.t1 | AT3G10600 | 37.900 | 438 | 247 | 8 | 92 | 511 | 1 | 431 | 7.19e-90 | 282 |
| MS.gene007811.t1 | AT5G04770 | 39.048 | 420 | 239 | 5 | 92 | 497 | 1 | 417 | 2.24e-85 | 271 |
| MS.gene007811.t1 | AT1G05940 | 30.827 | 532 | 332 | 10 | 1 | 507 | 48 | 568 | 2.72e-61 | 211 |
| MS.gene007811.t1 | AT5G36940 | 30.366 | 382 | 259 | 3 | 1 | 375 | 29 | 410 | 3.69e-58 | 203 |
| MS.gene007811.t1 | AT1G58030 | 30.105 | 382 | 260 | 3 | 1 | 375 | 40 | 421 | 1.05e-56 | 200 |
| MS.gene007811.t1 | AT1G05940 | 31.134 | 485 | 300 | 8 | 1 | 462 | 48 | 521 | 2.80e-56 | 196 |
| MS.gene007811.t1 | AT1G05940 | 31.873 | 411 | 267 | 4 | 1 | 399 | 48 | 457 | 1.80e-55 | 194 |
| MS.gene007811.t1 | AT3G03720 | 29.504 | 383 | 261 | 5 | 1 | 375 | 24 | 405 | 6.62e-52 | 186 |
| MS.gene007811.t1 | AT1G05940 | 29.011 | 455 | 286 | 9 | 77 | 507 | 9 | 450 | 2.03e-45 | 166 |
Find 114 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATATACCAATTGGTTTAAT+TGG | 0.142386 | 8.1:+53836794 | MS.gene007811:CDS |
| TTCTTTCCTTGCGGTGTTAA+AGG | 0.185948 | 8.1:+53836680 | MS.gene007811:CDS |
| CTTTATCATTGTGGCAAAAT+AGG | 0.190393 | 8.1:-53836432 | None:intergenic |
| ATTAATAGGTGTTCCAGTTT+TGG | 0.242325 | 8.1:-53837080 | None:intergenic |
| TTGATTGTGAGGAGATATTA+TGG | 0.270784 | 8.1:+53837220 | MS.gene007811:CDS |
| GGCATTGGCACTTGTGAATA+TGG | 0.273952 | 8.1:+53836853 | MS.gene007811:CDS |
| GGGACTGCGCTATGGAATTC+TGG | 0.274074 | 8.1:+53837319 | MS.gene007811:CDS |
| GTTGTTGGTTCTTGTGTTGC+TGG | 0.281893 | 8.1:+53837298 | MS.gene007811:CDS |
| GCCGTTGAAGTTCCTGTTGC+CGG | 0.312047 | 8.1:+53836293 | MS.gene007811:CDS |
| AACAAAACCACAACGGCTAA+TGG | 0.322847 | 8.1:-53836518 | None:intergenic |
| GCACTTATTCATCCCAAAAC+TGG | 0.324146 | 8.1:+53837067 | MS.gene007811:CDS |
| TTGTGTTTGTTTGTTGTTGT+TGG | 0.327089 | 8.1:+53837283 | MS.gene007811:CDS |
| TTGTTCAAATTGGTATGAAA+TGG | 0.328124 | 8.1:+53836921 | MS.gene007811:CDS |
| AACGGTTATTTATTGTTTGA+TGG | 0.330270 | 8.1:+53836832 | MS.gene007811:CDS |
| TGACAACAAGTTTGCTTGTT+GGG | 0.330599 | 8.1:+53836981 | MS.gene007811:CDS |
| CAACGGATAAGAGCGATTTA+TGG | 0.330824 | 8.1:+53837251 | MS.gene007811:CDS |
| CCAATGATCCCATAAGAAAA+AGG | 0.332205 | 8.1:-53837489 | None:intergenic |
| CTGCCGGTCTCGGTCGTTCA+TGG | 0.340474 | 8.1:+53836405 | MS.gene007811:CDS |
| CTTGTTGGTGCTGCCGGTCT+CGG | 0.343843 | 8.1:+53836395 | MS.gene007811:CDS |
| TGTTCAAATTGGTATGAAAT+GGG | 0.344658 | 8.1:+53836922 | MS.gene007811:CDS |
| TTCGGCGCTGTCGTTGGTTC+CGG | 0.347948 | 8.1:+53836155 | MS.gene007811:CDS |
| ATTCTCCTTGAAGCTCTTGT+TGG | 0.364163 | 8.1:+53836380 | MS.gene007811:CDS |
| TTTAGATTCTTGATTTGTAC+TGG | 0.365670 | 8.1:+53837526 | MS.gene007811:CDS |
| CTATGGAATTCTGGTTTGTT+TGG | 0.375852 | 8.1:+53837328 | MS.gene007811:CDS |
| CTCGCTTTCGGCGCTGTCGT+TGG | 0.378647 | 8.1:+53836149 | MS.gene007811:CDS |
| GTCAAAAGTGTAGCATTAAT+AGG | 0.382938 | 8.1:-53837094 | None:intergenic |
| GAAGAGGCAACCAAAGGTTT+GGG | 0.387706 | 8.1:+53837420 | MS.gene007811:CDS |
| ACCTTTGGTTGCCTCTTCTT+CGG | 0.388733 | 8.1:-53837415 | None:intergenic |
| TATGGGATCATTGGGTTATG+AGG | 0.391461 | 8.1:+53837498 | MS.gene007811:CDS |
| TCTGGTTTGTTTGGTTGGAT+TGG | 0.392722 | 8.1:+53837337 | MS.gene007811:CDS |
| CACCTTCCATTCTCTCATCA+TGG | 0.395056 | 8.1:-53837627 | None:intergenic |
| GGAATTCTGGTTTGTTTGGT+TGG | 0.396442 | 8.1:+53837332 | MS.gene007811:CDS |
| GACTTAACCTGGCTCGCTTT+CGG | 0.398071 | 8.1:+53836137 | MS.gene007811:CDS |
| AAGTTTGCTTGTTGGGTCAA+TGG | 0.398278 | 8.1:+53836988 | MS.gene007811:CDS |
| TTTCTTGCTTTCATTGCTGC+TGG | 0.398831 | 8.1:+53836353 | MS.gene007811:CDS |
| AGAAGAGGCAACCAAAGGTT+TGG | 0.405906 | 8.1:+53837419 | MS.gene007811:CDS |
| GAAAACGAGCCACCGGCAAC+AGG | 0.406244 | 8.1:-53836305 | None:intergenic |
| CCAATTGGTATATCTCTTGA+AGG | 0.410536 | 8.1:-53836785 | None:intergenic |
| GACAGCGCCGAAAGCGAGCC+AGG | 0.411784 | 8.1:-53836144 | None:intergenic |
| AATCACCGAGTTCGATTCTC+AGG | 0.413491 | 8.1:-53836331 | None:intergenic |
| ACGCTGCAGCTGTCGTGTAT+TGG | 0.414985 | 8.1:+53836711 | MS.gene007811:CDS |
| TATTTATTGTTTGATGGCAT+TGG | 0.415152 | 8.1:+53836838 | MS.gene007811:CDS |
| CCATGACTCGTTGCCTCACT+TGG | 0.415187 | 8.1:+53836111 | None:intergenic |
| TTGTTGGTTCTTGTGTTGCT+GGG | 0.417278 | 8.1:+53837299 | MS.gene007811:CDS |
| ATTGAACCAATTAAACCAAT+TGG | 0.417758 | 8.1:-53836800 | None:intergenic |
| AGTTTGCTTGTTGGGTCAAT+GGG | 0.420169 | 8.1:+53836989 | MS.gene007811:CDS |
| ATACCGTGGCTGCTTGTGTT+TGG | 0.437593 | 8.1:+53837362 | MS.gene007811:CDS |
| CGCTGCATATGAGAGGATAA+TGG | 0.438614 | 8.1:-53836225 | None:intergenic |
| ATCCTCTCATATGCAGCGTC+AGG | 0.442530 | 8.1:+53836230 | MS.gene007811:CDS |
| CGGCTTTAAAAGAATTGATC+CGG | 0.443728 | 8.1:-53836478 | None:intergenic |
| CCTTCAAGAGATATACCAAT+TGG | 0.445509 | 8.1:+53836785 | MS.gene007811:CDS |
| CAACAAATAGGGCTAAACTA+TGG | 0.448577 | 8.1:-53837650 | None:intergenic |
| TGGATCAAGCATATTGAAAC+CGG | 0.456149 | 8.1:-53836498 | None:intergenic |
| TCACTTGGTGGGACTTAACC+TGG | 0.456437 | 8.1:+53836126 | MS.gene007811:CDS |
| TGATTGTGAGGAGATATTAT+GGG | 0.459724 | 8.1:+53837221 | MS.gene007811:CDS |
| GAAGCTCTTGTTGGTGCTGC+CGG | 0.460471 | 8.1:+53836389 | MS.gene007811:CDS |
| CACAATGATAAAGAACGATT+CGG | 0.464761 | 8.1:+53836442 | MS.gene007811:CDS |
| TGGTTCAATGTCTATGATAA+CGG | 0.465812 | 8.1:+53836814 | MS.gene007811:CDS |
| ATCAAACCATGATGAGAGAA+TGG | 0.480689 | 8.1:+53837621 | MS.gene007811:CDS |
| GTCGTGTATTGGTCTTATAC+CGG | 0.481654 | 8.1:+53836722 | MS.gene007811:CDS |
| TCCGGTATCTTCGTCGTCAC+CGG | 0.482639 | 8.1:+53836173 | MS.gene007811:CDS |
| ACCGGTGACGACGAAGATAC+CGG | 0.483231 | 8.1:-53836174 | None:intergenic |
| GACTCGTTGCCTCACTTGGT+GGG | 0.485706 | 8.1:+53836115 | MS.gene007811:CDS |
| ATGACAACAAGTTTGCTTGT+TGG | 0.486739 | 8.1:+53836980 | MS.gene007811:CDS |
| TGACTCGTTGCCTCACTTGG+TGG | 0.488994 | 8.1:+53836114 | MS.gene007811:CDS |
| CCAAGTGAGGCAACGAGTCA+TGG | 0.489696 | 8.1:-53836111 | None:intergenic |
| GCAACTTATGATGTGGATCA+TGG | 0.490232 | 8.1:+53837586 | MS.gene007811:CDS |
| TATGATGTGGATCATGGAAT+TGG | 0.490780 | 8.1:+53837592 | MS.gene007811:CDS |
| AGTTGCAACCATATCAAAAC+CGG | 0.498318 | 8.1:-53836741 | None:intergenic |
| CGAATCGTTCTTTATCATTG+TGG | 0.500875 | 8.1:-53836441 | None:intergenic |
| CAAGAAGCTCGTTTCAATGC+TGG | 0.502149 | 8.1:+53836197 | MS.gene007811:CDS |
| GTGAGTATTTGTGCTTTGAA+AGG | 0.502564 | 8.1:+53836956 | MS.gene007811:CDS |
| CAGCACCAACAAGAGCTTCA+AGG | 0.507060 | 8.1:-53836385 | None:intergenic |
| GTTCAAATTGGTATGAAATG+GGG | 0.527507 | 8.1:+53836923 | MS.gene007811:CDS |
| GCTTGATCCATTAGCCGTTG+TGG | 0.536314 | 8.1:+53836511 | MS.gene007811:CDS |
| TGACCATGAACGACCGAGAC+CGG | 0.540172 | 8.1:-53836408 | None:intergenic |
| GTGTTGCTGGGACTGCGCTA+TGG | 0.541923 | 8.1:+53837311 | MS.gene007811:CDS |
| ATCGACAATGATGGCACCCA+TGG | 0.546905 | 8.1:-53837457 | None:intergenic |
| ATTGAAACGAGCTTCTTGAC+CGG | 0.547155 | 8.1:-53836192 | None:intergenic |
| TGAGTTTGCTTCCGAAGAAG+AGG | 0.551130 | 8.1:+53837404 | MS.gene007811:CDS |
| TTGGGTCAATGGGACAAGCT+AGG | 0.554068 | 8.1:+53836999 | MS.gene007811:CDS |
| CAATTGGTATATCTCTTGAA+GGG | 0.554114 | 8.1:-53836784 | None:intergenic |
| TGTGAGGAGATATTATGGGA+GGG | 0.562883 | 8.1:+53837225 | MS.gene007811:CDS |
| AAAACACCTTTAACACCGCA+AGG | 0.567101 | 8.1:-53836686 | None:intergenic |
| AAGAGGCAACCAAAGGTTTG+GGG | 0.576079 | 8.1:+53837421 | MS.gene007811:CDS |
| GTTAATCACTAACGGAATTG+CGG | 0.578401 | 8.1:+53836538 | MS.gene007811:CDS |
| TTGTGAGGAGATATTATGGG+AGG | 0.581589 | 8.1:+53837224 | MS.gene007811:CDS |
| AGAGGCAACCAAAGGTTTGG+GGG | 0.584028 | 8.1:+53837422 | MS.gene007811:CDS |
| TTAACACCGTTCTTTCCTTG+CGG | 0.585161 | 8.1:+53836671 | MS.gene007811:CDS |
| CAGGAATGAAAACGAGCCAC+CGG | 0.587952 | 8.1:-53836312 | None:intergenic |
| TCCGAAGAAGAGGCAACCAA+AGG | 0.591614 | 8.1:+53837414 | MS.gene007811:CDS |
| AACCATGATGAGAGAATGGA+AGG | 0.591931 | 8.1:+53837625 | MS.gene007811:CDS |
| GTTGAAGTTCCTGTTGCCGG+TGG | 0.592442 | 8.1:+53836296 | MS.gene007811:CDS |
| GATGGCACCCATGGAACCAA+CGG | 0.594938 | 8.1:-53837448 | None:intergenic |
| AAGTATAGTGAGATTGATGC+TGG | 0.595966 | 8.1:+53836878 | MS.gene007811:CDS |
| ATATTATGGGAGGGAATCAA+CGG | 0.605663 | 8.1:+53837234 | MS.gene007811:CDS |
| TGGCTATTGCGTTGATTGTG+AGG | 0.607141 | 8.1:+53837209 | MS.gene007811:CDS |
| TTCGTCGCAATCGACAATGA+TGG | 0.608279 | 8.1:-53837466 | None:intergenic |
| GGATAAGAGCGATTTATGGA+AGG | 0.608757 | 8.1:+53837255 | MS.gene007811:CDS |
| AAACCAAACACAAGCAGCCA+CGG | 0.609702 | 8.1:-53837365 | None:intergenic |
| ACTAACGGAATTGCGGCTAG+TGG | 0.614925 | 8.1:+53836545 | MS.gene007811:CDS |
| AGGTTAAGTCCCACCAAGTG+AGG | 0.616937 | 8.1:-53836124 | None:intergenic |
| AACCTGACGCTGCATATGAG+AGG | 0.617228 | 8.1:-53836232 | None:intergenic |
| ACCGGCAACAGGAACTTCAA+CGG | 0.628727 | 8.1:-53836294 | None:intergenic |
| TTAACACCGCAAGGAAAGAA+CGG | 0.633974 | 8.1:-53836677 | None:intergenic |
| TCATTCCTGAGAATCGAACT+CGG | 0.639210 | 8.1:+53836326 | MS.gene007811:CDS |
| GGTACACTACTCAAATTGCA+AGG | 0.653655 | 8.1:+53837020 | MS.gene007811:CDS |
| GAGGCAACCAAAGGTTTGGG+GGG | 0.658204 | 8.1:+53837423 | MS.gene007811:CDS |
| GCATATGAGAGGATAATGGA+AGG | 0.658306 | 8.1:-53836221 | None:intergenic |
| TGTTCATGCAACTTATGATG+TGG | 0.672047 | 8.1:+53837579 | MS.gene007811:CDS |
| GATGAAAGCGGAAAGAAGAG+CGG | 0.683982 | 8.1:-53836258 | None:intergenic |
| GAATTCAGCGTAGATGAAAG+CGG | 0.686074 | 8.1:-53836270 | None:intergenic |
| TGGTTGGATTGGTTATACCG+TGG | 0.704461 | 8.1:+53837348 | MS.gene007811:CDS |
| AGTGATTAACAAAACCACAA+CGG | 0.729960 | 8.1:-53836525 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTACAACTTTTATTAT+CGG | + | chr8.1:53836605-53836624 | MS.gene007811:CDS | 15.0% |
| !!! | CTTTTGTATTATTTATTTGT+GGG | + | chr8.1:53837556-53837575 | MS.gene007811:CDS | 15.0% |
| !!! | GCTTTTGTATTATTTATTTG+TGG | + | chr8.1:53837555-53837574 | MS.gene007811:CDS | 20.0% |
| !!! | TCTTTTCATTTTTATGCTTA+TGG | + | chr8.1:53837189-53837208 | MS.gene007811:CDS | 20.0% |
| ! | AACGGTTATTTATTGTTTGA+TGG | + | chr8.1:53836832-53836851 | MS.gene007811:CDS | 25.0% |
| ! | ATTGAACCAATTAAACCAAT+TGG | - | chr8.1:53836803-53836822 | None:intergenic | 25.0% |
| ! | GAAAAACATCTATCTTAACA+TGG | + | chr8.1:53836573-53836592 | MS.gene007811:CDS | 25.0% |
| ! | TGTTCAAATTGGTATGAAAT+GGG | + | chr8.1:53836922-53836941 | MS.gene007811:CDS | 25.0% |
| ! | TTGTTCAAATTGGTATGAAA+TGG | + | chr8.1:53836921-53836940 | MS.gene007811:CDS | 25.0% |
| !! | GATATACCAATTGGTTTAAT+TGG | + | chr8.1:53836794-53836813 | MS.gene007811:CDS | 25.0% |
| !! | TATTTATTGTTTGATGGCAT+TGG | + | chr8.1:53836838-53836857 | MS.gene007811:CDS | 25.0% |
| !!! | ATCGGTTTTATTATTGTGAT+CGG | + | chr8.1:53836623-53836642 | MS.gene007811:CDS | 25.0% |
| !!! | TTTAGATTCTTGATTTGTAC+TGG | + | chr8.1:53837526-53837545 | MS.gene007811:CDS | 25.0% |
| AGTGATTAACAAAACCACAA+CGG | - | chr8.1:53836528-53836547 | None:intergenic | 30.0% | |
| CAATTGGTATATCTCTTGAA+GGG | - | chr8.1:53836787-53836806 | None:intergenic | 30.0% | |
| CACAATGATAAAGAACGATT+CGG | + | chr8.1:53836442-53836461 | MS.gene007811:CDS | 30.0% | |
| CTTTATCATTGTGGCAAAAT+AGG | - | chr8.1:53836435-53836454 | None:intergenic | 30.0% | |
| GGATGAATAAGTGCAAAAAA+GGG | - | chr8.1:53837061-53837080 | None:intergenic | 30.0% | |
| GTTCAAATTGGTATGAAATG+GGG | + | chr8.1:53836923-53836942 | MS.gene007811:CDS | 30.0% | |
| TGATTGTGAGGAGATATTAT+GGG | + | chr8.1:53837221-53837240 | MS.gene007811:CDS | 30.0% | |
| TGGTTCAATGTCTATGATAA+CGG | + | chr8.1:53836814-53836833 | MS.gene007811:CDS | 30.0% | |
| TTGATTGTGAGGAGATATTA+TGG | + | chr8.1:53837220-53837239 | MS.gene007811:CDS | 30.0% | |
| ! | ATTAATAGGTGTTCCAGTTT+TGG | - | chr8.1:53837083-53837102 | None:intergenic | 30.0% |
| ! | CTTTTTCTTATGGGATCATT+GGG | + | chr8.1:53837490-53837509 | MS.gene007811:CDS | 30.0% |
| ! | GTCAAAAGTGTAGCATTAAT+AGG | - | chr8.1:53837097-53837116 | None:intergenic | 30.0% |
| ! | TGTTGCTCTTTTTACTAGTT+TGG | + | chr8.1:53837135-53837154 | MS.gene007811:CDS | 30.0% |
| ! | TTAATAGGTGTTCCAGTTTT+GGG | - | chr8.1:53837082-53837101 | None:intergenic | 30.0% |
| ! | TTTTGATATGGTTGCAACTA+TGG | + | chr8.1:53836745-53836764 | MS.gene007811:CDS | 30.0% |
| !! | AGAACGATTCGGATTATTTT+CGG | + | chr8.1:53836453-53836472 | MS.gene007811:CDS | 30.0% |
| !! | ATTGTGATCGGTTTTATACA+TGG | + | chr8.1:53836635-53836654 | MS.gene007811:CDS | 30.0% |
| !! | TTGTGTTTGTTTGTTGTTGT+TGG | + | chr8.1:53837283-53837302 | MS.gene007811:CDS | 30.0% |
| !!! | GTGGTTTTGTTAATCACTAA+CGG | + | chr8.1:53836530-53836549 | MS.gene007811:CDS | 30.0% |
| !!! | TCAGTTGCTTTTGTTCAAAT+TGG | + | chr8.1:53836911-53836930 | MS.gene007811:CDS | 30.0% |
| AAGTATAGTGAGATTGATGC+TGG | + | chr8.1:53836878-53836897 | MS.gene007811:CDS | 35.0% | |
| AGTTGCAACCATATCAAAAC+CGG | - | chr8.1:53836744-53836763 | None:intergenic | 35.0% | |
| ATATTATGGGAGGGAATCAA+CGG | + | chr8.1:53837234-53837253 | MS.gene007811:CDS | 35.0% | |
| ATCAAACCATGATGAGAGAA+TGG | + | chr8.1:53837621-53837640 | MS.gene007811:CDS | 35.0% | |
| CCAATGATCCCATAAGAAAA+AGG | - | chr8.1:53837492-53837511 | None:intergenic | 35.0% | |
| CCAATTGGTATATCTCTTGA+AGG | - | chr8.1:53836788-53836807 | None:intergenic | 35.0% | |
| CCTTCAAGAGATATACCAAT+TGG | + | chr8.1:53836785-53836804 | MS.gene007811:CDS | 35.0% | |
| CGAATCGTTCTTTATCATTG+TGG | - | chr8.1:53836444-53836463 | None:intergenic | 35.0% | |
| GGGATGAATAAGTGCAAAAA+AGG | - | chr8.1:53837062-53837081 | None:intergenic | 35.0% | |
| GTTAATCACTAACGGAATTG+CGG | + | chr8.1:53836538-53836557 | MS.gene007811:CDS | 35.0% | |
| TATGATGTGGATCATGGAAT+TGG | + | chr8.1:53837592-53837611 | MS.gene007811:CDS | 35.0% | |
| TGAATAAGTGCAAAAAAGGG+TGG | - | chr8.1:53837058-53837077 | None:intergenic | 35.0% | |
| TGGATCAAGCATATTGAAAC+CGG | - | chr8.1:53836501-53836520 | None:intergenic | 35.0% | |
| TGTTCATGCAACTTATGATG+TGG | + | chr8.1:53837579-53837598 | MS.gene007811:CDS | 35.0% | |
| ! | ATGACAACAAGTTTGCTTGT+TGG | + | chr8.1:53836980-53836999 | MS.gene007811:CDS | 35.0% |
| ! | ATTCGGATTATTTTCGGATC+CGG | + | chr8.1:53836459-53836478 | MS.gene007811:CDS | 35.0% |
| ! | CCTTTTTCTTATGGGATCAT+TGG | + | chr8.1:53837489-53837508 | MS.gene007811:CDS | 35.0% |
| ! | GTGAGTATTTGTGCTTTGAA+AGG | + | chr8.1:53836956-53836975 | MS.gene007811:CDS | 35.0% |
| ! | TGACAACAAGTTTGCTTGTT+GGG | + | chr8.1:53836981-53837000 | MS.gene007811:CDS | 35.0% |
| !! | CGGCTTTAAAAGAATTGATC+CGG | - | chr8.1:53836481-53836500 | None:intergenic | 35.0% |
| !! | CTATGGAATTCTGGTTTGTT+TGG | + | chr8.1:53837328-53837347 | MS.gene007811:CDS | 35.0% |
| !!! | CGGATCAATTCTTTTAAAGC+CGG | + | chr8.1:53836479-53836498 | MS.gene007811:CDS | 35.0% |
| !!! | GTCTTATACCGGTTTTGATA+TGG | + | chr8.1:53836733-53836752 | MS.gene007811:CDS | 35.0% |
| AAAACACCTTTAACACCGCA+AGG | - | chr8.1:53836689-53836708 | None:intergenic | 40.0% | |
| AACAAAACCACAACGGCTAA+TGG | - | chr8.1:53836521-53836540 | None:intergenic | 40.0% | |
| AACCATGATGAGAGAATGGA+AGG | + | chr8.1:53837625-53837644 | MS.gene007811:CDS | 40.0% | |
| ATTCTCCTTGAAGCTCTTGT+TGG | + | chr8.1:53836380-53836399 | MS.gene007811:CDS | 40.0% | |
| ATTGAAACGAGCTTCTTGAC+CGG | - | chr8.1:53836195-53836214 | None:intergenic | 40.0% | |
| CAACGGATAAGAGCGATTTA+TGG | + | chr8.1:53837251-53837270 | MS.gene007811:CDS | 40.0% | |
| GAATTCAGCGTAGATGAAAG+CGG | - | chr8.1:53836273-53836292 | None:intergenic | 40.0% | |
| GCAACTTATGATGTGGATCA+TGG | + | chr8.1:53837586-53837605 | MS.gene007811:CDS | 40.0% | |
| GCACTTATTCATCCCAAAAC+TGG | + | chr8.1:53837067-53837086 | MS.gene007811:CDS | 40.0% | |
| GGATAAGAGCGATTTATGGA+AGG | + | chr8.1:53837255-53837274 | MS.gene007811:CDS | 40.0% | |
| GGTACACTACTCAAATTGCA+AGG | + | chr8.1:53837020-53837039 | MS.gene007811:CDS | 40.0% | |
| TATGGGATCATTGGGTTATG+AGG | + | chr8.1:53837498-53837517 | MS.gene007811:CDS | 40.0% | |
| TCATTCCTGAGAATCGAACT+CGG | + | chr8.1:53836326-53836345 | MS.gene007811:CDS | 40.0% | |
| TGTGAGGAGATATTATGGGA+GGG | + | chr8.1:53837225-53837244 | MS.gene007811:CDS | 40.0% | |
| TTAACACCGCAAGGAAAGAA+CGG | - | chr8.1:53836680-53836699 | None:intergenic | 40.0% | |
| TTAACACCGTTCTTTCCTTG+CGG | + | chr8.1:53836671-53836690 | MS.gene007811:CDS | 40.0% | |
| TTGTGAGGAGATATTATGGG+AGG | + | chr8.1:53837224-53837243 | MS.gene007811:CDS | 40.0% | |
| TTTCTTGCTTTCATTGCTGC+TGG | + | chr8.1:53836353-53836372 | MS.gene007811:CDS | 40.0% | |
| ! | AAGTTTGCTTGTTGGGTCAA+TGG | + | chr8.1:53836988-53837007 | MS.gene007811:CDS | 40.0% |
| ! | AGTTTGCTTGTTGGGTCAAT+GGG | + | chr8.1:53836989-53837008 | MS.gene007811:CDS | 40.0% |
| ! | GCGACGAACCTTTTTCTTAT+GGG | + | chr8.1:53837481-53837500 | MS.gene007811:CDS | 40.0% |
| ! | TGCGACGAACCTTTTTCTTA+TGG | + | chr8.1:53837480-53837499 | MS.gene007811:CDS | 40.0% |
| ! | TTCTTTCCTTGCGGTGTTAA+AGG | + | chr8.1:53836680-53836699 | MS.gene007811:CDS | 40.0% |
| !! | GCATATGAGAGGATAATGGA+AGG | - | chr8.1:53836224-53836243 | None:intergenic | 40.0% |
| !! | GGAATTCTGGTTTGTTTGGT+TGG | + | chr8.1:53837332-53837351 | MS.gene007811:CDS | 40.0% |
| !! | GTCGTGTATTGGTCTTATAC+CGG | + | chr8.1:53836722-53836741 | MS.gene007811:CDS | 40.0% |
| !! | TCTGGTTTGTTTGGTTGGAT+TGG | + | chr8.1:53837337-53837356 | MS.gene007811:CDS | 40.0% |
| !! | TTGTTGGTTCTTGTGTTGCT+GGG | + | chr8.1:53837299-53837318 | MS.gene007811:CDS | 40.0% |
| !!! | TGTTTGGTTTTTGGCGACTT+TGG | + | chr8.1:53837378-53837397 | MS.gene007811:CDS | 40.0% |
| AAACCAAACACAAGCAGCCA+CGG | - | chr8.1:53837368-53837387 | None:intergenic | 45.0% | |
| AAGAGGCAACCAAAGGTTTG+GGG | + | chr8.1:53837421-53837440 | MS.gene007811:CDS | 45.0% | |
| AATCACCGAGTTCGATTCTC+AGG | - | chr8.1:53836334-53836353 | None:intergenic | 45.0% | |
| ACCTTTGGTTGCCTCTTCTT+CGG | - | chr8.1:53837418-53837437 | None:intergenic | 45.0% | |
| AGAAGAGGCAACCAAAGGTT+TGG | + | chr8.1:53837419-53837438 | MS.gene007811:CDS | 45.0% | |
| CAAGAAGCTCGTTTCAATGC+TGG | + | chr8.1:53836197-53836216 | MS.gene007811:CDS | 45.0% | |
| CACCTTCCATTCTCTCATCA+TGG | - | chr8.1:53837630-53837649 | None:intergenic | 45.0% | |
| CGCTGCATATGAGAGGATAA+TGG | - | chr8.1:53836228-53836247 | None:intergenic | 45.0% | |
| GAAGAGGCAACCAAAGGTTT+GGG | + | chr8.1:53837420-53837439 | MS.gene007811:CDS | 45.0% | |
| GATGAAAGCGGAAAGAAGAG+CGG | - | chr8.1:53836261-53836280 | None:intergenic | 45.0% | |
| TTCGTCGCAATCGACAATGA+TGG | - | chr8.1:53837469-53837488 | None:intergenic | 45.0% | |
| ! | TGAGTTTGCTTCCGAAGAAG+AGG | + | chr8.1:53837404-53837423 | MS.gene007811:CDS | 45.0% |
| !! | GGCATTGGCACTTGTGAATA+TGG | + | chr8.1:53836853-53836872 | MS.gene007811:CDS | 45.0% |
| !! | GTTGTTGGTTCTTGTGTTGC+TGG | + | chr8.1:53837298-53837317 | MS.gene007811:CDS | 45.0% |
| !! | TGGCTATTGCGTTGATTGTG+AGG | + | chr8.1:53837209-53837228 | MS.gene007811:CDS | 45.0% |
| !! | TGGTTGGATTGGTTATACCG+TGG | + | chr8.1:53837348-53837367 | MS.gene007811:CDS | 45.0% |
| !!! | GGCTGCTTGTGTTTGGTTTT+TGG | + | chr8.1:53837369-53837388 | MS.gene007811:CDS | 45.0% |
| AACCTGACGCTGCATATGAG+AGG | - | chr8.1:53836235-53836254 | None:intergenic | 50.0% | |
| ACCGGCAACAGGAACTTCAA+CGG | - | chr8.1:53836297-53836316 | None:intergenic | 50.0% | |
| ACTAACGGAATTGCGGCTAG+TGG | + | chr8.1:53836545-53836564 | MS.gene007811:CDS | 50.0% | |
| AGAGGCAACCAAAGGTTTGG+GGG | + | chr8.1:53837422-53837441 | MS.gene007811:CDS | 50.0% | |
| AGGTTAAGTCCCACCAAGTG+AGG | - | chr8.1:53836127-53836146 | None:intergenic | 50.0% | |
| ATACCGTGGCTGCTTGTGTT+TGG | + | chr8.1:53837362-53837381 | MS.gene007811:CDS | 50.0% | |
| ATCCTCTCATATGCAGCGTC+AGG | + | chr8.1:53836230-53836249 | MS.gene007811:CDS | 50.0% | |
| ATCGACAATGATGGCACCCA+TGG | - | chr8.1:53837460-53837479 | None:intergenic | 50.0% | |
| CAGCACCAACAAGAGCTTCA+AGG | - | chr8.1:53836388-53836407 | None:intergenic | 50.0% | |
| CAGGAATGAAAACGAGCCAC+CGG | - | chr8.1:53836315-53836334 | None:intergenic | 50.0% | |
| GCTTGATCCATTAGCCGTTG+TGG | + | chr8.1:53836511-53836530 | MS.gene007811:CDS | 50.0% | |
| TCCGAAGAAGAGGCAACCAA+AGG | + | chr8.1:53837414-53837433 | MS.gene007811:CDS | 50.0% | |
| TTGGGTCAATGGGACAAGCT+AGG | + | chr8.1:53836999-53837018 | MS.gene007811:CDS | 50.0% | |
| ! | GACTTAACCTGGCTCGCTTT+CGG | + | chr8.1:53836137-53836156 | MS.gene007811:CDS | 50.0% |
| ! | TCACTTGGTGGGACTTAACC+TGG | + | chr8.1:53836126-53836145 | MS.gene007811:CDS | 50.0% |
| AAAGGTTTGGGGGGTTCCGT+TGG | + | chr8.1:53837432-53837451 | MS.gene007811:CDS | 55.0% | |
| AACGGAACCCCCCAAACCTT+TGG | - | chr8.1:53837433-53837452 | None:intergenic | 55.0% | |
| ACCGGTGACGACGAAGATAC+CGG | - | chr8.1:53836177-53836196 | None:intergenic | 55.0% | |
| ACGCTGCAGCTGTCGTGTAT+TGG | + | chr8.1:53836711-53836730 | MS.gene007811:CDS | 55.0% | |
| GACTCGTTGCCTCACTTGGT+GGG | + | chr8.1:53836115-53836134 | MS.gene007811:CDS | 55.0% | |
| GAGGCAACCAAAGGTTTGGG+GGG | + | chr8.1:53837423-53837442 | MS.gene007811:CDS | 55.0% | |
| GATGGCACCCATGGAACCAA+CGG | - | chr8.1:53837451-53837470 | None:intergenic | 55.0% | |
| GCCGTTGAAGTTCCTGTTGC+CGG | + | chr8.1:53836293-53836312 | MS.gene007811:CDS | 55.0% | |
| GGGACTGCGCTATGGAATTC+TGG | + | chr8.1:53837319-53837338 | MS.gene007811:CDS | 55.0% | |
| GTTGAAGTTCCTGTTGCCGG+TGG | + | chr8.1:53836296-53836315 | MS.gene007811:CDS | 55.0% | |
| TCCGGTATCTTCGTCGTCAC+CGG | + | chr8.1:53836173-53836192 | MS.gene007811:CDS | 55.0% | |
| TGACCATGAACGACCGAGAC+CGG | - | chr8.1:53836411-53836430 | None:intergenic | 55.0% | |
| TGACTCGTTGCCTCACTTGG+TGG | + | chr8.1:53836114-53836133 | MS.gene007811:CDS | 55.0% | |
| !! | GAAGCTCTTGTTGGTGCTGC+CGG | + | chr8.1:53836389-53836408 | MS.gene007811:CDS | 55.0% |
| GAAAACGAGCCACCGGCAAC+AGG | - | chr8.1:53836308-53836327 | None:intergenic | 60.0% | |
| ! | GTGTTGCTGGGACTGCGCTA+TGG | + | chr8.1:53837311-53837330 | MS.gene007811:CDS | 60.0% |
| !! | CTTGTTGGTGCTGCCGGTCT+CGG | + | chr8.1:53836395-53836414 | MS.gene007811:CDS | 60.0% |
| !! | GGGGGTTCCGTTGGTTCCAT+GGG | + | chr8.1:53837441-53837460 | MS.gene007811:CDS | 60.0% |
| !! | TTCGGCGCTGTCGTTGGTTC+CGG | + | chr8.1:53836155-53836174 | MS.gene007811:CDS | 60.0% |
| CTCGCTTTCGGCGCTGTCGT+TGG | + | chr8.1:53836149-53836168 | MS.gene007811:CDS | 65.0% | |
| CTGCCGGTCTCGGTCGTTCA+TGG | + | chr8.1:53836405-53836424 | MS.gene007811:CDS | 65.0% | |
| !! | GGGGGGTTCCGTTGGTTCCA+TGG | + | chr8.1:53837440-53837459 | MS.gene007811:CDS | 65.0% |
| ! | GACAGCGCCGAAAGCGAGCC+AGG | - | chr8.1:53836147-53836166 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 53836113 | 53837660 | 53836113 | ID=MS.gene007811 |
| chr8.1 | mRNA | 53836113 | 53837660 | 53836113 | ID=MS.gene007811.t1;Parent=MS.gene007811 |
| chr8.1 | exon | 53836113 | 53837660 | 53836113 | ID=MS.gene007811.t1.exon1;Parent=MS.gene007811.t1 |
| chr8.1 | CDS | 53836113 | 53837660 | 53836113 | ID=cds.MS.gene007811.t1;Parent=MS.gene007811.t1 |
| Gene Sequence |
| Protein sequence |
