AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54227

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54227.t1 MTR_6g065270 92.398 342 22 1 1 338 1 342 0.0 661
MS.gene54227.t1 MTR_6g065370 80.480 333 65 0 6 338 8 340 0.0 575
MS.gene54227.t1 MTR_6g065400 78.979 333 70 0 6 338 8 340 0.0 567
MS.gene54227.t1 MTR_6g065120 77.844 334 73 1 6 338 9 342 0.0 560
MS.gene54227.t1 MTR_6g065340 81.381 333 46 1 6 338 19 335 0.0 541
MS.gene54227.t1 MTR_6g065110 67.879 330 105 1 8 336 11 340 2.38e-176 492
MS.gene54227.t1 MTR_6g065430 56.757 333 135 2 3 333 4 329 7.33e-141 402
MS.gene54227.t1 MTR_7g093950 37.059 340 205 4 1 332 9 347 7.69e-75 234
MS.gene54227.t1 MTR_1g082210 37.612 335 202 3 2 332 10 341 2.13e-74 233
MS.gene54227.t1 MTR_1g089310 37.685 337 201 3 1 332 9 341 2.83e-74 233
MS.gene54227.t1 MTR_1g089310 37.685 337 201 3 1 332 25 357 4.37e-74 233
MS.gene54227.t1 MTR_6g464280 37.591 274 157 6 30 296 24 290 2.65e-51 173
MS.gene54227.t1 MTR_8g035540 33.439 314 188 6 29 331 13 316 3.38e-47 162
MS.gene54227.t1 MTR_8g035500 32.492 317 197 6 23 331 10 317 3.26e-46 159
MS.gene54227.t1 MTR_4g050140 31.529 314 193 5 29 333 17 317 5.07e-46 159
MS.gene54227.t1 MTR_6g464510 35.870 276 159 8 29 296 27 292 2.09e-45 157
MS.gene54227.t1 MTR_7g406880 32.051 312 185 8 44 333 45 351 1.11e-44 156
MS.gene54227.t1 MTR_8g035520 33.779 299 176 6 47 334 40 327 2.30e-44 155
MS.gene54227.t1 MTR_6g464320 34.982 283 168 6 22 296 17 291 5.77e-44 154
MS.gene54227.t1 MTR_0007s0520 39.171 217 117 4 44 254 37 244 6.36e-44 154
MS.gene54227.t1 MTR_1g102160 37.269 271 143 10 29 285 23 280 8.35e-43 151
MS.gene54227.t1 MTR_1g012430 32.581 310 189 8 29 333 15 309 7.91e-42 148
MS.gene54227.t1 MTR_7g107040 32.313 294 178 5 53 336 39 321 2.57e-41 147
MS.gene54227.t1 MTR_3g012440 30.247 324 207 4 29 333 19 342 2.01e-40 145
MS.gene54227.t1 MTR_1g111280 32.343 303 174 11 45 331 51 338 9.72e-40 143
MS.gene54227.t1 MTR_1g102170 34.468 235 126 6 30 254 43 259 1.36e-39 142
MS.gene54227.t1 MTR_3g101420 32.558 301 171 9 47 333 34 316 4.33e-38 138
MS.gene54227.t1 MTR_6g464120 34.914 232 130 6 30 254 29 246 3.30e-37 140
MS.gene54227.t1 MTR_4g086480 31.864 295 176 9 47 331 39 318 1.77e-35 131
MS.gene54227.t1 MTR_4g086540 30.592 304 177 8 36 334 33 307 2.19e-35 131
MS.gene54227.t1 MTR_4g086510 30.847 295 184 7 47 332 35 318 2.41e-35 131
MS.gene54227.t1 MTR_7g056207 30.743 296 183 8 47 332 67 350 4.43e-33 125
MS.gene54227.t1 MTR_4g086470 31.313 297 179 9 47 333 38 319 2.29e-31 120
MS.gene54227.t1 MTR_1g105020 28.378 296 184 8 51 331 40 322 2.27e-29 115
MS.gene54227.t1 MTR_6g022710 29.818 275 147 5 89 322 156 425 3.31e-29 117
MS.gene54227.t1 MTR_3g450680 28.179 291 181 7 51 326 48 325 1.86e-27 110
MS.gene54227.t1 MTR_5g005850 28.523 298 182 8 51 331 45 328 4.15e-26 106
MS.gene54227.t1 MTR_5g005900 27.692 325 198 9 29 333 21 328 6.05e-26 105
MS.gene54227.t1 MTR_5g005920 26.708 322 205 10 29 333 25 332 3.79e-25 103
MS.gene54227.t1 MTR_7g051020 30.503 318 194 9 29 333 24 327 1.39e-24 102
MS.gene54227.t1 MTR_8g040860 26.923 312 197 11 29 326 28 322 2.24e-24 101
MS.gene54227.t1 MTR_6g065470 33.529 170 81 3 6 157 7 162 2.78e-22 92.0
MS.gene54227.t1 MTR_1g104930 29.467 319 193 11 29 333 24 324 2.76e-19 87.4
MS.gene54227.t1 MTR_1g104933 28.621 290 177 8 57 333 46 318 9.50e-18 83.2
MS.gene54227.t1 MTR_6g065480 53.448 58 26 1 231 288 1 57 2.64e-15 69.7
MS.gene54227.t1 MTR_0007s0480 38.462 91 50 2 169 254 1 90 5.27e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54227.t1 AT5G23530 55.000 340 131 5 2 333 3 328 5.85e-141 402
MS.gene54227.t1 AT5G27320 37.910 335 202 2 1 332 9 340 1.31e-78 244
MS.gene54227.t1 AT3G63010 35.652 345 210 5 1 338 9 348 1.63e-70 224
MS.gene54227.t1 AT3G05120 35.015 337 211 2 1 332 9 342 4.65e-70 222
MS.gene54227.t1 AT5G62180 34.824 313 189 10 30 334 21 326 6.11e-52 175
MS.gene54227.t1 AT5G16080 34.164 281 159 8 46 314 54 320 1.46e-44 156
MS.gene54227.t1 AT5G06570 35.088 342 187 9 9 335 8 329 2.38e-44 155
MS.gene54227.t1 AT5G06570 35.088 342 187 9 9 335 8 329 2.38e-44 155
MS.gene54227.t1 AT5G06570 35.088 342 187 9 9 335 23 344 3.42e-44 155
MS.gene54227.t1 AT5G06570 35.088 342 187 9 9 335 23 344 3.42e-44 155
MS.gene54227.t1 AT2G45600 32.313 294 175 6 57 337 39 321 4.99e-44 154
MS.gene54227.t1 AT2G45610 33.571 280 169 5 30 300 22 293 2.27e-43 152
MS.gene54227.t1 AT1G47480 31.307 329 200 10 11 334 5 312 7.34e-42 148
MS.gene54227.t1 AT1G68620 31.010 287 177 7 54 332 57 330 7.40e-42 149
MS.gene54227.t1 AT1G19190 30.915 317 187 13 30 333 18 315 1.67e-32 123
MS.gene54227.t1 AT3G48700 30.195 308 180 11 47 332 32 326 3.91e-32 122
MS.gene54227.t1 AT2G03550 31.879 298 175 9 47 333 32 312 1.71e-30 118
MS.gene54227.t1 AT5G14310 31.387 274 141 6 93 322 151 421 2.64e-30 120
MS.gene54227.t1 AT3G27320 27.368 380 183 9 23 322 37 403 5.67e-30 119
MS.gene54227.t1 AT3G48690 29.236 301 187 9 47 332 32 321 4.00e-29 114
MS.gene54227.t1 AT3G27320 29.904 311 166 8 54 322 135 435 4.08e-29 117
MS.gene54227.t1 AT1G49660 28.990 307 194 10 35 331 22 314 1.93e-26 107
MS.gene54227.t1 AT1G49640 33.708 178 109 5 50 225 36 206 6.01e-26 105
MS.gene54227.t1 AT1G49650 30.888 259 156 9 51 299 92 337 4.69e-22 95.9

Find 103 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACAGTTCCGGTGCAAATT+TGG 0.242565 6.2:+70404357 MS.gene54227:CDS
CTCCAGGAAATCCGGATTAT+TGG 0.243456 6.2:+70404415 MS.gene54227:CDS
GACAGGAAGGGAGGCTGTTT+TGG 0.273074 6.2:-70404092 None:intergenic
GGCACATCATCTTGCAGTTC+GGG 0.281818 6.2:+70404378 MS.gene54227:CDS
CTCTTTACTCCCACTGTTGC+CGG 0.296074 6.2:+70404043 MS.gene54227:CDS
TGCGGAGGATTTATCGGGTT+TGG 0.301727 6.2:+70404606 MS.gene54227:CDS
GGCGTTAACGGTAATGTCTT+TGG 0.307568 6.2:-70403999 None:intergenic
AGAGGCCGTGCTGAAATATT+TGG 0.323008 6.2:+70404276 MS.gene54227:CDS
TCCAGGAAATCCGGATTATT+GGG 0.333794 6.2:+70404416 MS.gene54227:CDS
GCCGAATGCGGAGGATTTAT+CGG 0.356800 6.2:+70404600 MS.gene54227:CDS
AATAATCAATCATTTAGAAT+TGG 0.363723 6.2:-70404833 None:intergenic
GGCAAAAGAGGTATTATGAT+TGG 0.376841 6.2:+70404680 MS.gene54227:CDS
ACCCAATAATCCGGATTTCC+TGG 0.387273 6.2:-70404417 None:intergenic
TTCCTTGCCGGAGACAGTTC+CGG 0.404209 6.2:+70404346 MS.gene54227:CDS
ATCATTTACTTCCACGGCGG+CGG 0.407976 6.2:+70404115 MS.gene54227:CDS
GATTTGATCCTTTGAATGAT+TGG 0.409071 6.2:+70404659 MS.gene54227:CDS
ACTCAACACCTCTTCTTCCT+TGG 0.411552 6.2:+70403837 MS.gene54227:CDS
TGGCACATCATCTTGCAGTT+CGG 0.418774 6.2:+70404377 MS.gene54227:CDS
GTGTCGAAATGTTTCCTTGC+CGG 0.420854 6.2:+70404334 MS.gene54227:CDS
TTTGGTGGAAACGCCGTTAA+CGG 0.430126 6.2:-70403981 None:intergenic
TGAGTAGATTCAGGCAGATC+AGG 0.435184 6.2:-70404772 None:intergenic
TAAAAGTGAAAGAAGTGAAA+TGG 0.440270 6.2:-70403873 None:intergenic
CCCGATAAATCCTCCGCATT+CGG 0.441988 6.2:-70404601 None:intergenic
GAGGGATCGAACCGGGATCA+TGG 0.444396 6.2:+70404559 MS.gene54227:CDS
CCTAACGCCACTCCCGTTAA+CGG 0.446230 6.2:+70403968 MS.gene54227:CDS
TTGGTGGAAACGCCGTTAAC+GGG 0.454826 6.2:-70403980 None:intergenic
CGCCGTTGGTGTCGATGGTA+AGG 0.455201 6.2:+70404506 MS.gene54227:CDS
GATATCGATGCTCCGGTGTA+AGG 0.456817 6.2:-70404234 None:intergenic
TATGATTGGTTAAAGAAATG+TGG 0.458980 6.2:+70404694 MS.gene54227:CDS
AAGCAGCGCTCCAGGAAATC+CGG 0.461952 6.2:+70404407 MS.gene54227:CDS
CTTTATGCCGTAGACTCTGC+AGG 0.467753 6.2:+70404176 MS.gene54227:CDS
GAGGCTGAGATTAAACTTGA+TGG 0.471710 6.2:+70404481 MS.gene54227:CDS
CCGAATGCGGAGGATTTATC+GGG 0.472619 6.2:+70404601 MS.gene54227:CDS
TTAACGCCGAAAATAACGTT+TGG 0.477196 6.2:+70404014 MS.gene54227:CDS
GGTTTGTAAAGCAGCGCTCC+AGG 0.482274 6.2:+70404399 MS.gene54227:CDS
CCGTTAACGGGAGTGGCGTT+AGG 0.486028 6.2:-70403968 None:intergenic
CTTTGAATGATTGGCAAAAG+AGG 0.487397 6.2:+70404668 MS.gene54227:CDS
CATGGAGCGGTTAATGTAAG+TGG 0.487420 6.2:+70404577 MS.gene54227:CDS
CCTGTCATCATTTACTTCCA+CGG 0.488327 6.2:+70404109 MS.gene54227:CDS
ATGATTGGTTAAAGAAATGT+GGG 0.488530 6.2:+70404695 MS.gene54227:CDS
TCATTTACTTCCACGGCGGC+GGG 0.488815 6.2:+70404116 MS.gene54227:CDS
AAGAAGTGAAATGGTAGCTC+TGG 0.489575 6.2:-70403864 None:intergenic
TCGATACCAACCCAATAATC+CGG 0.490985 6.2:-70404426 None:intergenic
ATTTGCACCGGAACTGTCTC+CGG 0.491985 6.2:-70404353 None:intergenic
TTTACCGGAGGGATCGAACC+GGG 0.500601 6.2:+70404552 MS.gene54227:CDS
GGAAACGCCGTTAACGGGAG+TGG 0.511016 6.2:-70403975 None:intergenic
CATTAACCGCTCCATGATCC+CGG 0.520430 6.2:-70404570 None:intergenic
CGATGGTAAGGACGGATTGG+TGG 0.521258 6.2:+70404518 MS.gene54227:CDS
AAATTTCCCTGCAGAGTCTA+CGG 0.521782 6.2:-70404183 None:intergenic
ATAATCAACTGAGTAGATTC+AGG 0.526163 6.2:-70404781 None:intergenic
GGAAATCCGGATTATTGGGT+TGG 0.530435 6.2:+70404420 MS.gene54227:CDS
TGGTAAGGACGGATTGGTGG+TGG 0.530541 6.2:+70404521 MS.gene54227:CDS
TGGATCGCCGTTGGTGTCGA+TGG 0.540273 6.2:+70404501 MS.gene54227:CDS
GTTAATGAGACGGCGGTTGA+CGG 0.543320 6.2:-70403924 None:intergenic
TAAAGCATCGTGGTAGATTG+AGG 0.544373 6.2:-70404158 None:intergenic
ATCGTGGTAGATTGAGGAAG+GGG 0.546376 6.2:-70404152 None:intergenic
AAGGACGGATTGGTGGTGGA+AGG 0.550023 6.2:+70404525 MS.gene54227:CDS
GCCGGAGAAGTCATCGGAGA+TGG 0.551507 6.2:+70404061 MS.gene54227:CDS
ACCATCTCCGATGACTTCTC+CGG 0.551868 6.2:-70404062 None:intergenic
ATATTTGGAAGAGAATAGAA+AGG 0.555206 6.2:+70404291 MS.gene54227:CDS
ATCAAGTTTAATCTCAGCCT+CGG 0.555342 6.2:-70404479 None:intergenic
TGCGACGGATATCGATGCTC+CGG 0.555954 6.2:-70404241 None:intergenic
CTCTTCCAAATATTTCAGCA+CGG 0.557249 6.2:-70404281 None:intergenic
CCGTGGAAGTAAATGATGAC+AGG 0.560772 6.2:-70404109 None:intergenic
TGATCCCGGTTCGATCCCTC+CGG 0.562811 6.2:-70404556 None:intergenic
CTGGCTTTCCAAGGAAGAAG+AGG 0.565695 6.2:-70403845 None:intergenic
GCATCGTGGTAGATTGAGGA+AGG 0.566906 6.2:-70404154 None:intergenic
GGCGCTTCTTCTCGCTCTAA+CGG 0.569447 6.2:+70403899 MS.gene54227:CDS
TATCCGTCGCAATATGATGA+TGG 0.571005 6.2:+70404253 MS.gene54227:CDS
TGTCGATGGTAAGGACGGAT+TGG 0.572977 6.2:+70404515 MS.gene54227:CDS
TAAACTTGATGGATCGCCGT+TGG 0.573185 6.2:+70404492 MS.gene54227:CDS
TGGCGGGGAGGATCGGACCG+AGG 0.573306 6.2:+70404462 MS.gene54227:CDS
GATGACTTCTCCGGCAACAG+TGG 0.577971 6.2:-70404053 None:intergenic
ATGGTAGCTCTGGCTTTCCA+AGG 0.587238 6.2:-70403854 None:intergenic
GGAAGTAAATGATGACAGGA+AGG 0.590018 6.2:-70404105 None:intergenic
GTTAACGGTAATGTCTTTGG+TGG 0.590282 6.2:-70403996 None:intergenic
CACCGGAACTGTCTCCGGCA+AGG 0.591057 6.2:-70404348 None:intergenic
GACTGAAGAAGTTAATGAGA+CGG 0.593190 6.2:-70403934 None:intergenic
ATGACTTCTCCGGCAACAGT+GGG 0.596379 6.2:-70404052 None:intergenic
TCAGTTGATTATGCAAGTCA+AGG 0.601932 6.2:+70404792 MS.gene54227:CDS
ATCGATGCTCCGGTGTAAGG+CGG 0.602490 6.2:-70404231 None:intergenic
ACTGTTGCCGGAGAAGTCAT+CGG 0.603429 6.2:+70404055 MS.gene54227:CDS
AACGGGAGTGGCGTTAGGTG+AGG 0.608356 6.2:-70403963 None:intergenic
GAAAACTGAACCCGCCGCCG+TGG 0.611082 6.2:-70404126 None:intergenic
ATGATGTGCCAAATTTGCAC+CGG 0.614788 6.2:-70404365 None:intergenic
GTCCTTACCATCGACACCAA+CGG 0.621799 6.2:-70404508 None:intergenic
TGTAAGTGGGCCGAATGCGG+AGG 0.622126 6.2:+70404591 MS.gene54227:CDS
GGAGAAGTCATCGGAGATGG+TGG 0.629162 6.2:+70404064 MS.gene54227:CDS
GTTGGTGTCGATGGTAAGGA+CGG 0.629245 6.2:+70404510 MS.gene54227:CDS
TAATGTAAGTGGGCCGAATG+CGG 0.629761 6.2:+70404588 MS.gene54227:CDS
ATGGAGCGGTTAATGTAAGT+GGG 0.631660 6.2:+70404578 MS.gene54227:CDS
GTAAATGATGACAGGAAGGG+AGG 0.632046 6.2:-70404101 None:intergenic
TGTCAACTACCGCCTTACAC+CGG 0.636801 6.2:+70404222 MS.gene54227:CDS
CATCGTGGTAGATTGAGGAA+GGG 0.643584 6.2:-70404153 None:intergenic
GTCGCAATATGATGATGGAG+AGG 0.652538 6.2:+70404258 MS.gene54227:CDS
GAAGTAAATGATGACAGGAA+GGG 0.653090 6.2:-70404104 None:intergenic
TCTCCATCATCATATTGCGA+CGG 0.653123 6.2:-70404256 None:intergenic
TTTATGCCGTAGACTCTGCA+GGG 0.653194 6.2:+70404177 MS.gene54227:CDS
GTTGATTGAATATCCAAACA+TGG 0.659983 6.2:+70404729 MS.gene54227:CDS
ATCGAACCGGGATCATGGAG+CGG 0.678664 6.2:+70404564 MS.gene54227:CDS
TGAAGAAGTTAATGAGACGG+CGG 0.714279 6.2:-70403931 None:intergenic
GTCATCATTTACTTCCACGG+CGG 0.732442 6.2:+70404112 MS.gene54227:CDS
GTCTACGGCATAAAGCATCG+TGG 0.766027 6.2:-70404168 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATTTGGAAGAGAATAGAA+AGG + chr6.2:70404291-70404310 MS.gene54227:CDS 25.0%
! TAAAAAACATGAACCATGTT+TGG - chr6.2:70404745-70404764 None:intergenic 25.0%
! TAAAAGTGAAAGAAGTGAAA+TGG - chr6.2:70403876-70403895 None:intergenic 25.0%
!! ATGATTGGTTAAAGAAATGT+GGG + chr6.2:70404695-70404714 MS.gene54227:CDS 25.0%
!! TATGATTGGTTAAAGAAATG+TGG + chr6.2:70404694-70404713 MS.gene54227:CDS 25.0%
!!! TCACTTCTTTCACTTTTATT+CGG + chr6.2:70403878-70403897 MS.gene54227:CDS 25.0%
ATAATCAACTGAGTAGATTC+AGG - chr6.2:70404784-70404803 None:intergenic 30.0%
GATTTGATCCTTTGAATGAT+TGG + chr6.2:70404659-70404678 MS.gene54227:CDS 30.0%
GTTGATTGAATATCCAAACA+TGG + chr6.2:70404729-70404748 MS.gene54227:CDS 30.0%
! AGAGAATAGAAAGGTTTTAC+CGG + chr6.2:70404300-70404319 MS.gene54227:CDS 30.0%
!! ACGAAACCAAACGTTATTTT+CGG - chr6.2:70404023-70404042 None:intergenic 30.0%
ATCAAGTTTAATCTCAGCCT+CGG - chr6.2:70404482-70404501 None:intergenic 35.0%
CTCTTCCAAATATTTCAGCA+CGG - chr6.2:70404284-70404303 None:intergenic 35.0%
CTTTGAATGATTGGCAAAAG+AGG + chr6.2:70404668-70404687 MS.gene54227:CDS 35.0%
GAAGTAAATGATGACAGGAA+GGG - chr6.2:70404107-70404126 None:intergenic 35.0%
GACTGAAGAAGTTAATGAGA+CGG - chr6.2:70403937-70403956 None:intergenic 35.0%
TCAGTTGATTATGCAAGTCA+AGG + chr6.2:70404792-70404811 MS.gene54227:CDS 35.0%
TTAACGCCGAAAATAACGTT+TGG + chr6.2:70404014-70404033 MS.gene54227:CDS 35.0%
! CTCTTTTGCCAATCATTCAA+AGG - chr6.2:70404670-70404689 None:intergenic 35.0%
! TTTATCGGGTTTGGATTTTC+CGG + chr6.2:70404615-70404634 MS.gene54227:CDS 35.0%
!! AACGTTATTTTCGGCGTTAA+CGG - chr6.2:70404014-70404033 None:intergenic 35.0%
!! GGCAAAAGAGGTATTATGAT+TGG + chr6.2:70404680-70404699 MS.gene54227:CDS 35.0%
AAATTTCCCTGCAGAGTCTA+CGG - chr6.2:70404186-70404205 None:intergenic 40.0%
AAGAAGTGAAATGGTAGCTC+TGG - chr6.2:70403867-70403886 None:intergenic 40.0%
ATGATGTGCCAAATTTGCAC+CGG - chr6.2:70404368-70404387 None:intergenic 40.0%
ATGGAGCGGTTAATGTAAGT+GGG + chr6.2:70404578-70404597 MS.gene54227:CDS 40.0%
CCAATAAAAACAAGCGTCTC+CGG - chr6.2:70404637-70404656 None:intergenic 40.0%
CCTGTCATCATTTACTTCCA+CGG + chr6.2:70404109-70404128 MS.gene54227:CDS 40.0%
GAGGCTGAGATTAAACTTGA+TGG + chr6.2:70404481-70404500 MS.gene54227:CDS 40.0%
GGAAGTAAATGATGACAGGA+AGG - chr6.2:70404108-70404127 None:intergenic 40.0%
GTTAACGGTAATGTCTTTGG+TGG - chr6.2:70403999-70404018 None:intergenic 40.0%
TATCCGTCGCAATATGATGA+TGG + chr6.2:70404253-70404272 MS.gene54227:CDS 40.0%
TCCAGGAAATCCGGATTATT+GGG + chr6.2:70404416-70404435 MS.gene54227:CDS 40.0%
TCGATACCAACCCAATAATC+CGG - chr6.2:70404429-70404448 None:intergenic 40.0%
TCTCCATCATCATATTGCGA+CGG - chr6.2:70404259-70404278 None:intergenic 40.0%
TGAAGAAGTTAATGAGACGG+CGG - chr6.2:70403934-70403953 None:intergenic 40.0%
!! TAAAGCATCGTGGTAGATTG+AGG - chr6.2:70404161-70404180 None:intergenic 40.0%
!!! GAGACGCTTGTTTTTATTGG+TGG + chr6.2:70404637-70404656 MS.gene54227:CDS 40.0%
!!! GTATCGATTCAGCCGTTTTT+TGG + chr6.2:70404442-70404461 MS.gene54227:CDS 40.0%
ACCCAATAATCCGGATTTCC+TGG - chr6.2:70404420-70404439 None:intergenic 45.0%
ACTCAACACCTCTTCTTCCT+TGG + chr6.2:70403837-70403856 MS.gene54227:CDS 45.0%
AGACAGTTCCGGTGCAAATT+TGG + chr6.2:70404357-70404376 MS.gene54227:CDS 45.0%
AGAGGCCGTGCTGAAATATT+TGG + chr6.2:70404276-70404295 MS.gene54227:CDS 45.0%
ATCGTGGTAGATTGAGGAAG+GGG - chr6.2:70404155-70404174 None:intergenic 45.0%
CATCGTGGTAGATTGAGGAA+GGG - chr6.2:70404156-70404175 None:intergenic 45.0%
CATGGAGCGGTTAATGTAAG+TGG + chr6.2:70404577-70404596 MS.gene54227:CDS 45.0%
CCGTGGAAGTAAATGATGAC+AGG - chr6.2:70404112-70404131 None:intergenic 45.0%
CTCCAGGAAATCCGGATTAT+TGG + chr6.2:70404415-70404434 MS.gene54227:CDS 45.0%
GGAAATCCGGATTATTGGGT+TGG + chr6.2:70404420-70404439 MS.gene54227:CDS 45.0%
GGCGTTAACGGTAATGTCTT+TGG - chr6.2:70404002-70404021 None:intergenic 45.0%
GTAAATGATGACAGGAAGGG+AGG - chr6.2:70404104-70404123 None:intergenic 45.0%
GTCATCATTTACTTCCACGG+CGG + chr6.2:70404112-70404131 MS.gene54227:CDS 45.0%
GTGTCGAAATGTTTCCTTGC+CGG + chr6.2:70404334-70404353 MS.gene54227:CDS 45.0%
TAATGTAAGTGGGCCGAATG+CGG + chr6.2:70404588-70404607 MS.gene54227:CDS 45.0%
TGAGTAGATTCAGGCAGATC+AGG - chr6.2:70404775-70404794 None:intergenic 45.0%
TGGCACATCATCTTGCAGTT+CGG + chr6.2:70404377-70404396 MS.gene54227:CDS 45.0%
TTTATGCCGTAGACTCTGCA+GGG + chr6.2:70404177-70404196 MS.gene54227:CDS 45.0%
TTTGGTGGAAACGCCGTTAA+CGG - chr6.2:70403984-70404003 None:intergenic 45.0%
! TAAACTTGATGGATCGCCGT+TGG + chr6.2:70404492-70404511 MS.gene54227:CDS 45.0%
! TTCGACACGTCAGCATTTTC+CGG - chr6.2:70404322-70404341 None:intergenic 45.0%
!! GTCGCAATATGATGATGGAG+AGG + chr6.2:70404258-70404277 MS.gene54227:CDS 45.0%
!!! CCGGAGACGCTTGTTTTTAT+TGG + chr6.2:70404634-70404653 MS.gene54227:CDS 45.0%
!!! GTGGTGGAAGGTGTTTTTAC+CGG + chr6.2:70404537-70404556 MS.gene54227:CDS 45.0%
!!! TCGATTCAGCCGTTTTTTGG+CGG + chr6.2:70404445-70404464 MS.gene54227:CDS 45.0%
!!! TGGAAGGTGTTTTTACCGGA+GGG + chr6.2:70404541-70404560 MS.gene54227:CDS 45.0%
ACCATCTCCGATGACTTCTC+CGG - chr6.2:70404065-70404084 None:intergenic 50.0%
ACTGTTGCCGGAGAAGTCAT+CGG + chr6.2:70404055-70404074 MS.gene54227:CDS 50.0%
ATCATTTACTTCCACGGCGG+CGG + chr6.2:70404115-70404134 MS.gene54227:CDS 50.0%
ATGACTTCTCCGGCAACAGT+GGG - chr6.2:70404055-70404074 None:intergenic 50.0%
ATTTGCACCGGAACTGTCTC+CGG - chr6.2:70404356-70404375 None:intergenic 50.0%
CATTAACCGCTCCATGATCC+CGG - chr6.2:70404573-70404592 None:intergenic 50.0%
CCCGATAAATCCTCCGCATT+CGG - chr6.2:70404604-70404623 None:intergenic 50.0%
CCGAATGCGGAGGATTTATC+GGG + chr6.2:70404601-70404620 MS.gene54227:CDS 50.0%
CTCTTTACTCCCACTGTTGC+CGG + chr6.2:70404043-70404062 MS.gene54227:CDS 50.0%
CTTTATGCCGTAGACTCTGC+AGG + chr6.2:70404176-70404195 MS.gene54227:CDS 50.0%
GATATCGATGCTCCGGTGTA+AGG - chr6.2:70404237-70404256 None:intergenic 50.0%
GCATCGTGGTAGATTGAGGA+AGG - chr6.2:70404157-70404176 None:intergenic 50.0%
GCCGAATGCGGAGGATTTAT+CGG + chr6.2:70404600-70404619 MS.gene54227:CDS 50.0%
GGCACATCATCTTGCAGTTC+GGG + chr6.2:70404378-70404397 MS.gene54227:CDS 50.0%
GTCCTTACCATCGACACCAA+CGG - chr6.2:70404511-70404530 None:intergenic 50.0%
GTTAATGAGACGGCGGTTGA+CGG - chr6.2:70403927-70403946 None:intergenic 50.0%
TGCGGAGGATTTATCGGGTT+TGG + chr6.2:70404606-70404625 MS.gene54227:CDS 50.0%
TGTCAACTACCGCCTTACAC+CGG + chr6.2:70404222-70404241 MS.gene54227:CDS 50.0%
TGTCGATGGTAAGGACGGAT+TGG + chr6.2:70404515-70404534 MS.gene54227:CDS 50.0%
TTGGTGGAAACGCCGTTAAC+GGG - chr6.2:70403983-70404002 None:intergenic 50.0%
! CTGGCTTTCCAAGGAAGAAG+AGG - chr6.2:70403848-70403867 None:intergenic 50.0%
! TTTTACCGGAGGGATCGAAC+CGG + chr6.2:70404551-70404570 MS.gene54227:CDS 50.0%
!! ATGGTAGCTCTGGCTTTCCA+AGG - chr6.2:70403857-70403876 None:intergenic 50.0%
!! GTCTACGGCATAAAGCATCG+TGG - chr6.2:70404171-70404190 None:intergenic 50.0%
!! GTTGGTGTCGATGGTAAGGA+CGG + chr6.2:70404510-70404529 MS.gene54227:CDS 50.0%
!!! CGATTCAGCCGTTTTTTGGC+GGG + chr6.2:70404446-70404465 MS.gene54227:CDS 50.0%
!!! GATTCAGCCGTTTTTTGGCG+GGG + chr6.2:70404447-70404466 MS.gene54227:CDS 50.0%
!!! GTGGAAGGTGTTTTTACCGG+AGG + chr6.2:70404540-70404559 MS.gene54227:CDS 50.0%
AAGCAGCGCTCCAGGAAATC+CGG + chr6.2:70404407-70404426 MS.gene54227:CDS 55.0%
ATCGAACCGGGATCATGGAG+CGG + chr6.2:70404564-70404583 MS.gene54227:CDS 55.0%
ATCGATGCTCCGGTGTAAGG+CGG - chr6.2:70404234-70404253 None:intergenic 55.0%
CCTAACGCCACTCCCGTTAA+CGG + chr6.2:70403968-70403987 MS.gene54227:CDS 55.0%
CGATCCTCCCCGCCAAAAAA+CGG - chr6.2:70404457-70404476 None:intergenic 55.0%
GATGACTTCTCCGGCAACAG+TGG - chr6.2:70404056-70404075 None:intergenic 55.0%
GGAGAAGTCATCGGAGATGG+TGG + chr6.2:70404064-70404083 MS.gene54227:CDS 55.0%
GGCGCTTCTTCTCGCTCTAA+CGG + chr6.2:70403899-70403918 MS.gene54227:CDS 55.0%
GGTTTGTAAAGCAGCGCTCC+AGG + chr6.2:70404399-70404418 MS.gene54227:CDS 55.0%
TCATTTACTTCCACGGCGGC+GGG + chr6.2:70404116-70404135 MS.gene54227:CDS 55.0%
TGCGACGGATATCGATGCTC+CGG - chr6.2:70404244-70404263 None:intergenic 55.0%
TTCCTTGCCGGAGACAGTTC+CGG + chr6.2:70404346-70404365 MS.gene54227:CDS 55.0%
TTTACCGGAGGGATCGAACC+GGG + chr6.2:70404552-70404571 MS.gene54227:CDS 55.0%
! GACAGGAAGGGAGGCTGTTT+TGG - chr6.2:70404095-70404114 None:intergenic 55.0%
!! AAGGACGGATTGGTGGTGGA+AGG + chr6.2:70404525-70404544 MS.gene54227:CDS 55.0%
!! AGGAAGGGAGGCTGTTTTGG+TGG - chr6.2:70404092-70404111 None:intergenic 55.0%
!! CGATGGTAAGGACGGATTGG+TGG + chr6.2:70404518-70404537 MS.gene54227:CDS 55.0%
!! TGGTAAGGACGGATTGGTGG+TGG + chr6.2:70404521-70404540 MS.gene54227:CDS 55.0%
!!! CGTTTTTTGGCGGGGAGGAT+CGG + chr6.2:70404455-70404474 MS.gene54227:CDS 55.0%
AACGGGAGTGGCGTTAGGTG+AGG - chr6.2:70403966-70403985 None:intergenic 60.0%
CCGTTAACGGGAGTGGCGTT+AGG - chr6.2:70403971-70403990 None:intergenic 60.0%
GAGGGATCGAACCGGGATCA+TGG + chr6.2:70404559-70404578 MS.gene54227:CDS 60.0%
GCCGGAGAAGTCATCGGAGA+TGG + chr6.2:70404061-70404080 MS.gene54227:CDS 60.0%
GGAAACGCCGTTAACGGGAG+TGG - chr6.2:70403978-70403997 None:intergenic 60.0%
TGTAAGTGGGCCGAATGCGG+AGG + chr6.2:70404591-70404610 MS.gene54227:CDS 60.0%
! TGATCCCGGTTCGATCCCTC+CGG - chr6.2:70404559-70404578 None:intergenic 60.0%
!! CGCCGTTGGTGTCGATGGTA+AGG + chr6.2:70404506-70404525 MS.gene54227:CDS 60.0%
!! TGGATCGCCGTTGGTGTCGA+TGG + chr6.2:70404501-70404520 MS.gene54227:CDS 60.0%
!!! TCAGCCGTTTTTTGGCGGGG+AGG + chr6.2:70404450-70404469 MS.gene54227:CDS 60.0%
CACCGGAACTGTCTCCGGCA+AGG - chr6.2:70404351-70404370 None:intergenic 65.0%
GAAAACTGAACCCGCCGCCG+TGG - chr6.2:70404129-70404148 None:intergenic 65.0%
TGGCGGGGAGGATCGGACCG+AGG + chr6.2:70404462-70404481 MS.gene54227:CDS 75.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 70403830 70404846 70403830 ID=MS.gene54227
chr6.2 mRNA 70403830 70404846 70403830 ID=MS.gene54227.t1;Parent=MS.gene54227
chr6.2 exon 70403830 70404846 70403830 ID=MS.gene54227.t1.exon1;Parent=MS.gene54227.t1
chr6.2 CDS 70403830 70404846 70403830 ID=cds.MS.gene54227.t1;Parent=MS.gene54227.t1
Gene Sequence

>MS.gene54227

ATGTCAGACTCAACACCTCTTCTTCCTTGGAAAGCCAGAGCTACCATTTCACTTCTTTCACTTTTATTCGGCGCTTCTTCTCGCTCTAACGGCACCGTCAACCGCCGTCTCATTAACTTCTTCAGTCTCAATGCCTCACCTAACGCCACTCCCGTTAACGGCGTTTCCACCAAAGACATTACCGTTAACGCCGAAAATAACGTTTGGTTTCGTCTCTTTACTCCCACTGTTGCCGGAGAAGTCATCGGAGATGGTGGTGCCACCAAAACAGCCTCCCTTCCTGTCATCATTTACTTCCACGGCGGCGGGTTCAGTTTTCTTTCCCCTTCCTCAATCTACCACGATGCTTTATGCCGTAGACTCTGCAGGGAAATTTTTGCTGTTGTCGTCTCTGTCAACTACCGCCTTACACCGGAGCATCGATATCCGTCGCAATATGATGATGGAGAGGCCGTGCTGAAATATTTGGAAGAGAATAGAAAGGTTTTACCGGAAAATGCTGACGTGTCGAAATGTTTCCTTGCCGGAGACAGTTCCGGTGCAAATTTGGCACATCATCTTGCAGTTCGGGTTTGTAAAGCAGCGCTCCAGGAAATCCGGATTATTGGGTTGGTATCGATTCAGCCGTTTTTTGGCGGGGAGGATCGGACCGAGGCTGAGATTAAACTTGATGGATCGCCGTTGGTGTCGATGGTAAGGACGGATTGGTGGTGGAAGGTGTTTTTACCGGAGGGATCGAACCGGGATCATGGAGCGGTTAATGTAAGTGGGCCGAATGCGGAGGATTTATCGGGTTTGGATTTTCCGGAGACGCTTGTTTTTATTGGTGGATTTGATCCTTTGAATGATTGGCAAAAGAGGTATTATGATTGGTTAAAGAAATGTGGGAAAAAAGCTGAGTTGATTGAATATCCAAACATGGTTCATGTTTTTTATGTTTTTCCTGATCTGCCTGAATCTACTCAGTTGATTATGCAAGTCAAGGAGTTTGTCAACAAAGTGTCCAATTCTAAATGA
Protein sequence

>MS.gene54227.t1

MSDSTPLLPWKARATISLLSLLFGASSRSNGTVNRRLINFFSLNASPNATPVNGVSTKDITVNAENNVWFRLFTPTVAGEVIGDGGATKTASLPVIIYFHGGGFSFLSPSSIYHDALCRRLCREIFAVVVSVNYRLTPEHRYPSQYDDGEAVLKYLEENRKVLPENADVSKCFLAGDSSGANLAHHLAVRVCKAALQEIRIIGLVSIQPFFGGEDRTEAEIKLDGSPLVSMVRTDWWWKVFLPEGSNRDHGAVNVSGPNAEDLSGLDFPETLVFIGGFDPLNDWQKRYYDWLKKCGKKAELIEYPNMVHVFYVFPDLPESTQLIMQVKEFVNKVSNSK