AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56161

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56161.t1 MTR_2g038380 94.350 354 17 1 1 354 1 351 0.0 652
MS.gene56161.t1 MTR_4g127120 72.871 317 80 3 40 352 24 338 1.33e-160 453
MS.gene56161.t1 MTR_4g127120 35.152 165 104 2 106 269 20 182 6.40e-13 69.3
MS.gene56161.t1 MTR_4g105010 47.303 241 126 1 47 287 208 447 2.28e-58 197
MS.gene56161.t1 MTR_4g105010 30.061 163 111 2 43 203 273 434 2.33e-11 65.1
MS.gene56161.t1 MTR_3g076990 49.083 218 110 1 70 287 228 444 5.72e-55 188
MS.gene56161.t1 MTR_3g076990 33.129 163 106 2 43 203 270 431 2.53e-12 68.2
MS.gene56161.t1 MTR_5g032910 43.952 248 138 1 40 287 148 394 7.42e-50 173
MS.gene56161.t1 MTR_8g072950 39.773 264 152 3 47 305 294 555 1.44e-42 155
MS.gene56161.t1 MTR_5g024780 40.167 239 141 2 47 285 305 541 2.63e-42 155
MS.gene56161.t1 MTR_5g024780 40.351 171 99 2 99 269 264 431 4.11e-27 112
MS.gene56161.t1 MTR_4g098530 38.346 266 139 3 44 286 198 461 5.27e-41 150
MS.gene56161.t1 MTR_4g098530 36.782 174 107 2 99 272 183 353 2.07e-22 98.6
MS.gene56161.t1 MTR_3g023830 30.804 224 124 6 46 266 24 219 8.07e-16 77.0
MS.gene56161.t1 MTR_3g023830 27.586 203 121 3 72 274 27 203 4.27e-14 72.0
MS.gene56161.t1 MTR_8g062130 32.500 240 156 6 49 286 796 1031 8.33e-16 79.3
MS.gene56161.t1 MTR_8g062130 34.031 191 119 6 75 263 729 914 6.74e-13 70.5
MS.gene56161.t1 MTR_8g062130 32.673 202 120 7 47 235 887 1085 1.42e-11 66.6
MS.gene56161.t1 MTR_1g038890 30.112 269 169 13 56 310 237 500 9.50e-16 79.3
MS.gene56161.t1 MTR_1g038890 29.018 224 145 8 49 267 94 308 1.51e-12 69.3
MS.gene56161.t1 MTR_8g062140 33.803 213 127 6 54 264 770 970 1.92e-15 78.2
MS.gene56161.t1 MTR_8g062140 36.441 236 110 10 51 260 698 919 8.15e-14 73.2
MS.gene56161.t1 MTR_8g062140 32.984 191 122 6 75 263 688 874 5.36e-12 67.8
MS.gene56161.t1 MTR_8g047220 36.444 225 129 13 58 273 155 374 3.30e-15 77.4
MS.gene56161.t1 MTR_8g047220 32.227 211 103 8 82 263 116 315 3.25e-11 65.1
MS.gene56161.t1 MTR_8g047220 31.304 230 141 11 57 272 201 427 5.60e-11 64.3
MS.gene56161.t1 MTR_2g079560 31.280 211 140 5 63 272 114 320 5.88e-15 76.6
MS.gene56161.t1 MTR_2g079560 27.347 245 149 5 47 288 409 627 2.41e-13 71.6
MS.gene56161.t1 MTR_2g079560 31.280 211 140 5 63 272 67 273 1.68e-14 75.1
MS.gene56161.t1 MTR_2g079560 27.347 245 149 5 47 288 364 582 5.59e-13 70.5
MS.gene56161.t1 MTR_2g079560 31.280 211 140 5 63 272 67 273 1.69e-14 75.1
MS.gene56161.t1 MTR_2g079560 27.347 245 149 5 47 288 362 580 5.58e-13 70.5
MS.gene56161.t1 MTR_1g040615 33.803 213 131 10 63 268 119 328 3.58e-14 74.7
MS.gene56161.t1 MTR_1g040615 29.960 247 162 11 35 273 138 381 4.84e-11 64.7
MS.gene56161.t1 MTR_4g028090 35.071 211 126 11 70 272 150 357 3.86e-14 74.3
MS.gene56161.t1 MTR_8g047230 36.792 212 124 10 63 267 254 462 6.41e-14 73.6
MS.gene56161.t1 MTR_8g047230 32.927 246 131 12 56 269 127 370 1.70e-13 72.4
MS.gene56161.t1 MTR_8g047230 33.019 212 129 10 62 267 114 318 4.58e-11 64.7
MS.gene56161.t1 MTR_1g040545 33.175 211 131 10 63 266 119 326 8.97e-14 73.2
MS.gene56161.t1 MTR_1g097580 33.632 223 137 10 52 266 79 298 9.55e-14 73.2
MS.gene56161.t1 MTR_6g038760 30.078 256 161 10 21 267 58 304 1.37e-13 71.6
MS.gene56161.t1 MTR_7g007560 36.022 186 107 8 90 267 90 271 2.13e-13 72.0
MS.gene56161.t1 MTR_2g079560 27.347 245 149 5 47 288 339 557 2.41e-13 71.6
MS.gene56161.t1 MTR_2g079560 31.383 188 125 4 86 272 64 248 1.87e-12 68.6
MS.gene56161.t1 MTR_7g045860 32.056 287 134 13 67 295 76 359 3.12e-13 71.6
MS.gene56161.t1 MTR_1g039220 31.963 219 141 8 56 268 213 429 3.30e-13 71.2
MS.gene56161.t1 MTR_1g039220 31.905 210 135 8 64 267 101 308 8.90e-12 67.0
MS.gene56161.t1 MTR_1g101250 26.490 302 183 8 19 284 405 703 4.99e-13 70.9
MS.gene56161.t1 MTR_2g450870 33.333 228 111 10 60 249 261 485 1.17e-12 69.7
MS.gene56161.t1 MTR_1g080440 28.016 257 150 11 44 268 78 331 1.59e-12 69.3
MS.gene56161.t1 MTR_8g469830 34.759 187 115 7 35 215 242 427 1.62e-12 69.3
MS.gene56161.t1 MTR_2g437730 33.014 209 129 11 67 267 503 708 2.58e-12 68.6
MS.gene56161.t1 MTR_4g130390 31.401 207 135 7 67 267 356 561 2.85e-12 68.6
MS.gene56161.t1 MTR_1415s0010 30.542 203 129 7 70 265 141 338 3.06e-12 67.4
MS.gene56161.t1 MTR_8g041100 40.000 155 86 7 67 215 206 359 3.91e-12 68.2
MS.gene56161.t1 MTR_8g047210 34.061 229 133 12 58 272 140 364 4.02e-12 68.2
MS.gene56161.t1 MTR_8g047210 32.432 222 109 9 82 273 101 311 7.47e-12 67.0
MS.gene56161.t1 MTR_3g465570 29.064 203 134 5 73 269 136 334 4.69e-12 67.0
MS.gene56161.t1 MTR_5g096340 37.736 159 91 8 63 215 281 437 5.89e-12 67.4
MS.gene56161.t1 MTR_2g017495 31.200 250 150 12 36 265 432 679 6.95e-12 67.4
MS.gene56161.t1 MTR_0491s0020 28.253 269 175 12 19 272 138 403 8.91e-12 66.2
MS.gene56161.t1 MTR_0491s0020 34.518 197 112 9 82 272 116 301 6.50e-11 63.5
MS.gene56161.t1 MTR_0491s0020 32.735 223 116 11 58 273 155 350 9.23e-11 63.2
MS.gene56161.t1 MTR_5g085700 33.493 209 112 8 63 268 132 316 1.02e-11 66.6
MS.gene56161.t1 MTR_1g102500 32.407 216 136 9 57 265 316 528 1.17e-11 66.6
MS.gene56161.t1 MTR_1g039240 35.052 194 117 9 67 254 586 776 1.25e-11 66.6
MS.gene56161.t1 MTR_1g039240 30.088 226 148 10 50 268 305 527 2.17e-11 65.9
MS.gene56161.t1 MTR_3g093930 30.769 221 139 9 56 265 269 486 1.55e-11 66.2
MS.gene56161.t1 MTR_7g010000 35.514 214 124 11 63 267 133 341 1.85e-11 65.9
MS.gene56161.t1 MTR_6g088785 27.148 291 156 10 12 274 25 287 1.97e-11 65.9
MS.gene56161.t1 MTR_7g045510 30.796 289 138 14 67 296 85 370 1.97e-11 65.9
MS.gene56161.t1 MTR_7g045710 31.359 287 136 13 67 295 85 368 2.09e-11 65.9
MS.gene56161.t1 MTR_6g088785 27.148 291 156 10 12 274 25 287 2.21e-11 65.9
MS.gene56161.t1 MTR_0830s0010 35.366 164 99 7 67 225 428 589 3.07e-11 65.1
MS.gene56161.t1 MTR_1g039310 34.536 194 118 9 67 254 583 773 3.27e-11 65.1
MS.gene56161.t1 MTR_3g030980 41.176 102 58 2 160 260 577 677 3.61e-11 65.1
MS.gene56161.t1 MTR_2g074990 30.612 245 152 13 35 267 98 336 3.71e-11 65.1
MS.gene56161.t1 MTR_0491s0030 34.884 215 130 10 56 263 100 311 4.21e-11 64.7
MS.gene56161.t1 MTR_1g040575 32.759 232 145 11 43 266 211 439 4.60e-11 64.7
MS.gene56161.t1 MTR_7g007630 29.851 268 159 11 14 267 39 291 5.04e-11 64.7
MS.gene56161.t1 MTR_3g033240 45.349 86 46 1 174 259 595 679 5.56e-11 64.7
MS.gene56161.t1 MTR_7g067530 31.336 217 140 9 56 265 371 585 5.58e-11 64.3
MS.gene56161.t1 MTR_7g073290 31.963 219 135 8 57 265 337 551 6.76e-11 64.3
MS.gene56161.t1 MTR_2g072610 30.348 201 124 9 80 267 265 462 7.40e-11 63.9
MS.gene56161.t1 MTR_4g070950 29.064 203 136 8 67 264 409 608 7.77e-11 63.9
MS.gene56161.t1 MTR_6g036870 29.319 191 126 8 90 273 272 460 8.68e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56161.t1 AT2G19330 64.151 318 109 2 40 352 58 375 6.13e-139 400
MS.gene56161.t1 AT4G26050 61.846 325 114 5 37 351 49 373 1.12e-125 366
MS.gene56161.t1 AT4G29880 61.950 318 116 2 40 352 51 368 2.95e-125 365
MS.gene56161.t1 AT3G11330 49.180 244 123 1 44 287 200 442 9.64e-58 195
MS.gene56161.t1 AT3G11330 32.298 161 106 2 43 201 268 427 1.71e-11 65.9
MS.gene56161.t1 AT5G05850 48.400 250 128 1 41 290 203 451 8.36e-57 193
MS.gene56161.t1 AT3G26500 40.856 257 139 2 44 300 162 405 3.97e-51 177
MS.gene56161.t1 AT1G12970 40.467 257 140 2 44 300 163 406 1.02e-48 171
MS.gene56161.t1 AT1G12970 35.294 187 110 6 35 215 200 381 1.93e-11 65.5
MS.gene56161.t1 AT4G35470 38.168 262 157 3 47 305 274 533 2.47e-42 155
MS.gene56161.t1 AT4G35470 39.196 199 107 3 98 285 232 427 1.47e-22 99.4
MS.gene56161.t1 AT2G17440 39.924 263 152 3 47 305 258 518 4.05e-40 148
MS.gene56161.t1 AT4G35470 40.171 234 138 2 47 280 274 505 2.24e-39 147
MS.gene56161.t1 AT4G35470 39.196 199 107 3 98 285 232 427 1.79e-22 99.0
MS.gene56161.t1 AT3G15410 34.450 209 132 4 63 270 72 276 3.73e-18 86.3
MS.gene56161.t1 AT3G15410 34.783 207 130 4 67 272 53 255 6.77e-18 85.5
MS.gene56161.t1 AT3G15410 28.723 282 166 6 46 298 78 353 2.41e-16 80.9
MS.gene56161.t1 AT3G15410 28.879 232 132 5 83 288 363 587 8.98e-14 72.8
MS.gene56161.t1 AT3G15410 35.071 211 128 5 63 270 66 270 3.84e-18 86.3
MS.gene56161.t1 AT3G15410 34.783 207 130 4 67 272 47 249 6.84e-18 85.5
MS.gene56161.t1 AT3G15410 28.723 282 166 6 46 298 72 347 2.57e-16 80.5
MS.gene56161.t1 AT3G15410 28.879 232 132 5 83 288 357 581 1.01e-13 72.8
MS.gene56161.t1 AT2G30105 34.211 190 109 5 101 278 141 326 2.88e-16 79.7
MS.gene56161.t1 AT2G30105 41.964 112 63 2 70 181 207 316 7.62e-15 75.5
MS.gene56161.t1 AT2G34930 33.766 231 136 10 47 263 204 431 1.12e-14 75.9
MS.gene56161.t1 AT1G27170 29.817 218 123 6 67 258 767 980 2.62e-14 75.1
MS.gene56161.t1 AT4G28490 34.409 186 112 9 70 247 119 302 3.03e-14 74.7
MS.gene56161.t1 AT1G27170 30.415 217 123 5 67 258 767 980 3.20e-14 74.7
MS.gene56161.t1 AT5G07910 27.586 232 137 6 46 274 25 228 8.12e-14 71.2
MS.gene56161.t1 AT1G69550 39.171 217 121 11 53 262 920 1132 9.31e-14 73.2
MS.gene56161.t1 AT1G69550 38.660 194 110 9 75 262 871 1061 3.20e-13 71.6
MS.gene56161.t1 AT1G69550 38.739 222 124 11 47 260 985 1202 6.62e-13 70.5
MS.gene56161.t1 AT1G69550 30.888 259 146 9 35 262 733 989 2.14e-11 65.9
MS.gene56161.t1 AT1G69550 33.818 275 156 13 53 317 1016 1274 5.51e-11 64.7
MS.gene56161.t1 AT3G56370 33.721 258 134 11 42 265 69 323 1.52e-13 72.4
MS.gene56161.t1 AT3G56370 32.911 237 142 9 36 263 230 458 1.47e-12 69.3
MS.gene56161.t1 AT2G15080 33.632 223 132 12 53 267 104 318 1.69e-13 72.4
MS.gene56161.t1 AT2G15080 33.632 223 132 12 53 267 104 318 1.69e-13 72.4
MS.gene56161.t1 AT5G44700 33.766 231 141 11 44 265 266 493 2.96e-13 71.6
MS.gene56161.t1 AT5G44700 30.342 234 154 9 17 243 599 830 9.36e-12 67.0
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 945 1154 1.03e-12 70.1
MS.gene56161.t1 AT1G27180 30.500 200 133 6 64 260 913 1109 7.94e-11 64.3
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 783 992 1.12e-12 70.1
MS.gene56161.t1 AT1G27180 30.500 200 133 6 64 260 751 947 8.07e-11 64.3
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 783 992 1.16e-12 69.7
MS.gene56161.t1 AT1G27180 30.631 222 144 7 49 265 830 1046 1.62e-11 66.2
MS.gene56161.t1 AT1G27180 30.500 200 133 6 64 260 751 947 8.09e-11 63.9
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 781 990 1.51e-12 69.7
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 781 990 1.51e-12 69.7
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 607 816 1.96e-12 69.3
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 607 816 1.96e-12 69.3
MS.gene56161.t1 AT1G27180 29.577 213 144 6 49 258 607 816 1.96e-12 69.3
MS.gene56161.t1 AT3G24240 35.484 217 130 10 56 265 289 502 2.62e-12 68.6
MS.gene56161.t1 AT4G04220 33.333 243 140 12 37 263 74 310 3.14e-12 68.2
MS.gene56161.t1 AT5G46330 34.222 225 115 10 74 268 178 399 4.72e-12 67.8
MS.gene56161.t1 AT5G46330 34.222 225 115 10 74 268 178 399 4.72e-12 67.8
MS.gene56161.t1 AT5G07180 33.803 213 132 9 67 273 75 284 5.61e-12 67.8
MS.gene56161.t1 AT4G13920 30.542 203 130 9 65 258 79 279 1.07e-11 66.6
MS.gene56161.t1 AT3G11010 32.189 233 145 10 39 265 124 349 1.33e-11 66.6
MS.gene56161.t1 AT3G11010 32.189 233 145 10 39 265 61 286 1.39e-11 66.2
MS.gene56161.t1 AT5G17680 26.587 252 145 6 45 260 762 1009 2.83e-11 65.5
MS.gene56161.t1 AT5G17680 26.587 252 145 6 45 260 761 1008 2.91e-11 65.5
MS.gene56161.t1 AT5G61480 31.556 225 143 10 51 267 114 335 3.43e-11 65.1
MS.gene56161.t1 AT3G47570 32.927 246 154 11 91 328 338 580 3.46e-11 65.1
MS.gene56161.t1 AT2G24130 34.247 219 134 10 57 267 337 553 4.33e-11 65.1
MS.gene56161.t1 AT2G24130 34.247 219 134 10 57 267 352 568 4.63e-11 64.7
MS.gene56161.t1 AT5G11250 34.328 268 158 16 50 307 603 862 4.71e-11 64.7
MS.gene56161.t1 AT5G11250 34.328 268 158 16 50 307 603 862 4.71e-11 64.7
MS.gene56161.t1 AT1G75640 29.126 206 136 9 70 267 293 496 5.57e-11 64.7
MS.gene56161.t1 AT5G11250 34.328 268 158 16 50 307 685 944 5.58e-11 64.7
MS.gene56161.t1 AT4G20140 30.435 230 125 11 70 267 580 806 8.05e-11 64.3
MS.gene56161.t1 AT5G27060 30.622 209 117 7 63 267 119 303 9.60e-11 63.9
MS.gene56161.t1 AT5G27060 30.622 209 117 7 63 267 119 303 9.60e-11 63.9
MS.gene56161.t1 AT5G27060 30.622 209 117 7 63 267 120 304 9.77e-11 63.9

Find 111 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATTCTGACTCACATTT+AGG 0.163400 2.3:+50611814 None:intergenic
CACCATCCACCATCTCTTCT+TGG 0.191377 2.3:+50611554 None:intergenic
TTCCCTCCACGCTTAGCTTT+TGG 0.217550 2.3:+50611676 None:intergenic
GAGTCAGAATTTCCGGTATT+TGG 0.246732 2.3:-50611805 MS.gene56161:CDS
GTTGAGATTCAGTTCTTCTA+AGG 0.278250 2.3:+50612034 None:intergenic
TAAATGTGAGTCAGAATTTC+CGG 0.296177 2.3:-50611812 MS.gene56161:CDS
CAGCAACCATTGAGAATTGC+AGG 0.306044 2.3:-50613765 MS.gene56161:intron
AGAGAGACAACCAATGGAAT+TGG 0.315520 2.3:+50613834 None:intergenic
GAAATTCTGACTCACATTTA+GGG 0.316541 2.3:+50611815 None:intergenic
CAATGGTTGCTGGGAGGGAT+TGG 0.332249 2.3:+50613776 None:intergenic
TCTTGCTTCACCAGGCTTCA+TGG 0.336493 2.3:-50611442 MS.gene56161:CDS
CTTGCTTCACCAGGCTTCAT+GGG 0.358409 2.3:-50611441 MS.gene56161:CDS
TCCACTGGAGGTGGTGGAGC+AGG 0.362764 2.3:-50611637 MS.gene56161:CDS
GCTAAATCAAGAGAAAGTTT+TGG 0.364003 2.3:+50614641 None:intergenic
CTGCAATTCTCAATGGTTGC+TGG 0.367867 2.3:+50613766 None:intergenic
CCCGCTCAGTTCGCCTCCAC+TGG 0.372267 2.3:-50611652 MS.gene56161:CDS
CTCAAAGCTTTGAATGTCTC+TGG 0.381783 2.3:-50613808 MS.gene56161:CDS
TCTTCTTGGTTGGGATATGA+TGG 0.383328 2.3:+50611568 None:intergenic
CTCTCACATGATGACTCTTA+TGG 0.393115 2.3:-50611907 MS.gene56161:CDS
ATCCACCATCTCTTCTTGGT+TGG 0.393964 2.3:+50611558 None:intergenic
ATTATTAAACTTGGAGATGT+TGG 0.403539 2.3:-50613887 MS.gene56161:CDS
GGACTTGCATGGCATGTCCT+TGG 0.409480 2.3:-50614672 MS.gene56161:CDS
ACTTATCATGTCTCCATTGA+AGG 0.410083 2.3:+50611521 None:intergenic
TGGTGAAGCAAGACCATTCA+TGG 0.416329 2.3:+50611452 None:intergenic
TTCGCCTCCACTGGAGGTGG+TGG 0.417968 2.3:-50611643 MS.gene56161:CDS
TCCACCATCTCTTCTTGGTT+GGG 0.419645 2.3:+50611559 None:intergenic
CAACAATATCAAAACATTGC+CGG 0.423660 2.3:-50611724 MS.gene56161:CDS
ACGAGGTTCTCAAGGTCGTC+GGG 0.432286 2.3:+50611849 None:intergenic
AGCTCAAAGCCTATTGTGTC+TGG 0.435730 2.3:+50611990 None:intergenic
TGGGAAACGATCAGAACATA+AGG 0.438511 2.3:-50611499 MS.gene56161:CDS
GAGAGACAACCAATGGAATT+GGG 0.443595 2.3:+50613835 None:intergenic
CTTCACCAGGCTTCATGGGG+AGG 0.443666 2.3:-50611437 MS.gene56161:CDS
TTCAATGGAGACATGATAAG+TGG 0.444496 2.3:-50611519 MS.gene56161:CDS
AGCAAACTTCCAGACACAAT+AGG 0.451995 2.3:-50611999 MS.gene56161:CDS
ATTCTCAATGGTTGCTGGGA+GGG 0.461311 2.3:+50613771 None:intergenic
ATTGTTAGACAAATTGAGCT+TGG 0.462966 2.3:+50614610 None:intergenic
AACGGCAAGATTATTAAACT+TGG 0.472327 2.3:-50613896 MS.gene56161:CDS
CCACTGGAGGTGGTGGAGCA+GGG 0.480559 2.3:-50611636 MS.gene56161:CDS
AGGAAGACACACAAGTTTGT+TGG 0.489389 2.3:+50611938 None:intergenic
GACAACCTCCCCATGAAGCC+TGG 0.490735 2.3:+50611432 None:intergenic
TGCAATTCTCAATGGTTGCT+GGG 0.491491 2.3:+50613767 None:intergenic
TGGAGGCATCCGATGGAGTC+CGG 0.491902 2.3:+50611705 None:intergenic
CCAGTGGAGGCGAACTGAGC+GGG 0.492506 2.3:+50611652 None:intergenic
GAACATACCGGAGTCATTAA+CGG 0.493750 2.3:-50613914 MS.gene56161:CDS
GTGAAATGTGGGACCTTCAA+TGG 0.499860 2.3:-50611534 MS.gene56161:CDS
CAGTTCGCCTCCACTGGAGG+TGG 0.507058 2.3:-50611646 MS.gene56161:CDS
GAGTCATTGCCCTACTCCAT+AGG 0.508724 2.3:-50611783 MS.gene56161:CDS
AATAAACCTGCAATTCTCAA+TGG 0.511036 2.3:+50613759 None:intergenic
TCAACTTAAGGAATTACGAC+GGG 0.513441 2.3:+50611389 None:intergenic
CAAGATTGACGAGGTTCTCA+AGG 0.516518 2.3:+50611841 None:intergenic
CAAGAGGAGTCCTATGGAGT+AGG 0.516632 2.3:+50611773 None:intergenic
TCATCAGATGACAATGAAGA+TGG 0.519446 2.3:-50614774 MS.gene56161:CDS
CTTCATTATCAACCCATGAA+TGG 0.521629 2.3:-50611465 MS.gene56161:CDS
ATGAATGGTCTTGCTTCACC+AGG 0.524055 2.3:-50611450 MS.gene56161:CDS
TGCTCCACCACCTCCAGTGG+AGG 0.525602 2.3:+50611639 None:intergenic
GAGCTTCTGGAGGCATCCGA+TGG 0.527574 2.3:+50611698 None:intergenic
CTATCTTCCTCCGTCGGCGT+CGG 0.529882 2.3:+50614707 None:intergenic
CCCTGCTCCACCACCTCCAG+TGG 0.531840 2.3:+50611636 None:intergenic
GAAGACGCCGCCGACGCCGA+CGG 0.534937 2.3:-50614717 MS.gene56161:CDS
TTTGTCTAACAATAATCTCC+AGG 0.539269 2.3:-50614600 MS.gene56161:intron
CTCCAAAAGCTAAGCGTGGA+GGG 0.545563 2.3:-50611678 MS.gene56161:CDS
AATTACGACGGGGTGAGAAG+AGG 0.549684 2.3:+50611400 None:intergenic
ACGTACGTTTGAATTGCCTC+AGG 0.550757 2.3:-50611878 MS.gene56161:CDS
GACGAGGTTCTCAAGGTCGT+CGG 0.550775 2.3:+50611848 None:intergenic
AGATCCCTACCCAATTCCAT+TGG 0.551883 2.3:-50613844 MS.gene56161:CDS
TTGCTTCACCAGGCTTCATG+GGG 0.551908 2.3:-50611440 MS.gene56161:CDS
AAGAGGAGTCCTATGGAGTA+GGG 0.556187 2.3:+50611774 None:intergenic
TAGGGACAAGAGGAGTCCTA+TGG 0.559709 2.3:+50611767 None:intergenic
GACAACCAATGGAATTGGGT+AGG 0.561937 2.3:+50613839 None:intergenic
ATCATATGACAACTAGGAAG+AGG 0.567301 2.3:-50614751 MS.gene56161:CDS
AGCTAACGTCTAGTTCAACT+AGG 0.568083 2.3:+50611748 None:intergenic
AAAACATTGCCGGACTCCAT+CGG 0.568332 2.3:-50611714 MS.gene56161:CDS
GCTCAGTTCGCCTCCACTGG+AGG 0.570036 2.3:-50611649 MS.gene56161:CDS
AGATGGTGGATGGTGAAATG+TGG 0.570372 2.3:-50611546 MS.gene56161:CDS
ATCATGTGAGAGGTGGAGCT+AGG 0.579817 2.3:+50611918 None:intergenic
ACAACCAATGGAATTGGGTA+GGG 0.580388 2.3:+50613840 None:intergenic
GGGAAACGATCAGAACATAA+GGG 0.581219 2.3:-50611498 MS.gene56161:CDS
AGGGCAATGACTCCAAATAC+CGG 0.582603 2.3:+50611793 None:intergenic
GACGCCGCCGACGCCGACGG+AGG 0.587900 2.3:-50614714 MS.gene56161:CDS
AATCTTGCCGTTAATGACTC+CGG 0.589085 2.3:+50613907 None:intergenic
GAGTTTAGAGAGACAACCAA+TGG 0.592305 2.3:+50613828 None:intergenic
AGATGGATCATATGACAACT+AGG 0.595001 2.3:-50614757 MS.gene56161:CDS
CGGGGTGAGAAGAGGCGAAG+AGG 0.596579 2.3:+50611408 None:intergenic
TCTTCCTCCGTCGGCGTCGG+CGG 0.598751 2.3:+50614710 None:intergenic
TCATATGACAACTAGGAAGA+GGG 0.604678 2.3:-50614750 MS.gene56161:CDS
GCTCCAAAAGCTAAGCGTGG+AGG 0.605699 2.3:-50611679 MS.gene56161:CDS
ATATCCCAACCAAGAAGAGA+TGG 0.606320 2.3:-50611563 MS.gene56161:CDS
AACCAAGAAGAGATGGTGGA+TGG 0.610943 2.3:-50611556 MS.gene56161:CDS
GTTCATGTTGTGGACTTGCA+TGG 0.612500 2.3:-50614683 MS.gene56161:CDS
CTAGTTCAACTAGGGACAAG+AGG 0.617875 2.3:+50611757 None:intergenic
TCAATGGAGACATGATAAGT+GGG 0.620791 2.3:-50611518 MS.gene56161:CDS
AAGAGTCATCATGTGAGAGG+TGG 0.622685 2.3:+50611911 None:intergenic
TCCCAACCAAGAAGAGATGG+TGG 0.632111 2.3:-50611560 MS.gene56161:CDS
AATTGGGTAGGGATCTGAGC+TGG 0.634161 2.3:+50613851 None:intergenic
GCTAACGTCTAGTTCAACTA+GGG 0.636021 2.3:+50611749 None:intergenic
CTCAACTTAAGGAATTACGA+CGG 0.637737 2.3:+50611388 None:intergenic
GGGTAGGGATCTGAGCTGGT+TGG 0.642111 2.3:+50613855 None:intergenic
GGTCGTCGGGGAGACTCCTG+AGG 0.642718 2.3:+50611862 None:intergenic
GGTGAAGCAAGACCATTCAT+GGG 0.642951 2.3:+50611453 None:intergenic
CATAAGAGTCATCATGTGAG+AGG 0.643496 2.3:+50611908 None:intergenic
TGAACTCTATCTTCCTCCGT+CGG 0.645634 2.3:+50614701 None:intergenic
GAAGCTCCAAAAGCTAAGCG+TGG 0.653198 2.3:-50611682 MS.gene56161:CDS
GATGGTGGATGGTGAAATGT+GGG 0.654566 2.3:-50611545 MS.gene56161:CDS
AATTCTCAATGGTTGCTGGG+AGG 0.654863 2.3:+50613770 None:intergenic
GGAAGATAGAGTTCATGTTG+TGG 0.654967 2.3:-50614693 MS.gene56161:CDS
TCCAGTGGAGGCGAACTGAG+CGG 0.662649 2.3:+50611651 None:intergenic
GGTGGAGCAGGGATTGCATG+TGG 0.678013 2.3:-50611625 MS.gene56161:CDS
CTATATATAACAGAACATAC+CGG 0.683092 2.3:-50613926 MS.gene56161:intron
CGAGGTTCTCAAGGTCGTCG+GGG 0.683613 2.3:+50611850 None:intergenic
CAACTTAAGGAATTACGACG+GGG 0.695800 2.3:+50611390 None:intergenic
TTAGGGTTTCAAGATTGACG+AGG 0.703892 2.3:+50611832 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAGATGTAATATAA+CGG - chr2.3:50612015-50612034 MS.gene56161:CDS 10.0%
!! TGTTATATAAATTATTTGAT+AGG - chr2.3:50612238-50612257 MS.gene56161:intron 10.0%
!!! TTTATTCTTGTTTTTTTTTA+GGG - chr2.3:50613705-50613724 MS.gene56161:intron 10.0%
!!! TTTTATTCTTGTTTTTTTTT+AGG - chr2.3:50613704-50613723 MS.gene56161:intron 10.0%
!!! TTTTTTTGAAAGAATTTTTA+GGG - chr2.3:50613736-50613755 MS.gene56161:intron 10.0%
!!! TTTTTTTTGAAAGAATTTTT+AGG - chr2.3:50613735-50613754 MS.gene56161:intron 10.0%
!! AAATTATCCTTATTAACTAT+TGG - chr2.3:50612674-50612693 MS.gene56161:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr2.3:50612675-50612694 None:intergenic 15.0%
!! CCATTATTATATGATAAAAT+TGG - chr2.3:50614014-50614033 MS.gene56161:intron 15.0%
!! TATTAACAAATTACATTTGT+AGG - chr2.3:50613413-50613432 MS.gene56161:intron 15.0%
!! TTATTCAATCAATATCTAAT+TGG + chr2.3:50613952-50613971 None:intergenic 15.0%
!!! AAAGATTATTCTATTTGTTA+TGG + chr2.3:50611847-50611866 None:intergenic 15.0%
!!! AATTTGTTAATATAAGTCTT+TGG + chr2.3:50613405-50613424 None:intergenic 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr2.3:50612801-50612820 MS.gene56161:intron 15.0%
!!! CCAATTTTATCATATAATAA+TGG + chr2.3:50614017-50614036 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - chr2.3:50612698-50612717 MS.gene56161:intron 15.0%
!!! TTATTCTTGTTTTTTTTTAG+GGG - chr2.3:50613706-50613725 MS.gene56161:intron 15.0%
!!! TTTTTTGAAAGAATTTTTAG+GGG - chr2.3:50613737-50613756 MS.gene56161:intron 15.0%
!! AAACAAGAATAAAAGTCTAT+CGG + chr2.3:50613698-50613717 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr2.3:50612684-50612703 None:intergenic 20.0%
!! AATAGAATAATCTTTGTTGA+AGG - chr2.3:50611852-50611871 MS.gene56161:CDS 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr2.3:50612761-50612780 MS.gene56161:intron 20.0%
!! ATAGAATAATCTTTGTTGAA+GGG - chr2.3:50611853-50611872 MS.gene56161:CDS 20.0%
!! TGATTAGAATTGAAAATTTG+AGG + chr2.3:50613861-50613880 None:intergenic 20.0%
!!! AGTACAATGCAATTTTATTA+AGG - chr2.3:50611660-50611679 MS.gene56161:CDS 20.0%
!!! TTATTAAGGTTTTGAAGTTT+AGG - chr2.3:50611674-50611693 MS.gene56161:CDS 20.0%
!!! TTGCATAACTTTTTTTTTAG+TGG - chr2.3:50613008-50613027 MS.gene56161:intron 20.0%
! AAAAACGAGAAATTCATGAT+GGG + chr2.3:50613805-50613824 None:intergenic 25.0%
! AACAAATATTGAACCAGAAT+TGG + chr2.3:50613609-50613628 None:intergenic 25.0%
! ACAAATATTGAACCAGAATT+GGG + chr2.3:50613608-50613627 None:intergenic 25.0%
! ACTAACCATGATTAAAAATC+CGG - chr2.3:50613671-50613690 MS.gene56161:intron 25.0%
! ATTATTAAACTTGGAGATGT+TGG - chr2.3:50612314-50612333 MS.gene56161:intron 25.0%
! CTATATATAACAGAACATAC+CGG - chr2.3:50612275-50612294 MS.gene56161:intron 25.0%
! TAAAAACGAGAAATTCATGA+TGG + chr2.3:50613806-50613825 None:intergenic 25.0%
! TCAATCAATATCTAATTGGT+AGG + chr2.3:50613948-50613967 None:intergenic 25.0%
! TCCAAATCTTAAAATCATAG+TGG - chr2.3:50611812-50611831 MS.gene56161:CDS 25.0%
! TGTGCATAGTATTAATTAGA+GGG - chr2.3:50612727-50612746 MS.gene56161:intron 25.0%
! TTTATTTGCATGACATCATT+AGG + chr2.3:50613487-50613506 None:intergenic 25.0%
!!! ACATGTTTTTAGTTGTGTAT+AGG - chr2.3:50612897-50612916 MS.gene56161:intron 25.0%
!!! ACCACTATGATTTTAAGATT+TGG + chr2.3:50611816-50611835 None:intergenic 25.0%
!!! ATGATTAGATAACTTGTTTG+AGG + chr2.3:50613335-50613354 None:intergenic 25.0%
!!! GGATTTTTTTGATAAGTATC+TGG - chr2.3:50611901-50611920 MS.gene56161:CDS 25.0%
!!! TTTAAAATGAGTGTCGTTTT+AGG - chr2.3:50612593-50612612 MS.gene56161:intron 25.0%
AAAACGAGAAATTCATGATG+GGG + chr2.3:50613804-50613823 None:intergenic 30.0%
AACGGCAAGATTATTAAACT+TGG - chr2.3:50612305-50612324 MS.gene56161:intron 30.0%
AATAAACCTGCAATTCTCAA+TGG + chr2.3:50612445-50612464 None:intergenic 30.0%
AATCTTTGTTGAAGGGAAAA+TGG - chr2.3:50611860-50611879 MS.gene56161:CDS 30.0%
AATTGTGAATTTGTCAACCT+TGG + chr2.3:50611635-50611654 None:intergenic 30.0%
ATTGTTAGACAAATTGAGCT+TGG + chr2.3:50611594-50611613 None:intergenic 30.0%
CAACAATATCAAAACATTGC+CGG - chr2.3:50614477-50614496 MS.gene56161:intron 30.0%
GAAATTCTGACTCACATTTA+GGG + chr2.3:50614389-50614408 None:intergenic 30.0%
GCAAAACACAATAATGAACA+TGG + chr2.3:50612992-50613011 None:intergenic 30.0%
GTGTGCATAGTATTAATTAG+AGG - chr2.3:50612726-50612745 MS.gene56161:intron 30.0%
TAAATGTGAGTCAGAATTTC+CGG - chr2.3:50614389-50614408 MS.gene56161:intron 30.0%
TTTGTCTAACAATAATCTCC+AGG - chr2.3:50611601-50611620 MS.gene56161:CDS 30.0%
! ACCCATATCAAGCTAATTTT+AGG - chr2.3:50612549-50612568 MS.gene56161:intron 30.0%
! CCCATATCAAGCTAATTTTA+GGG - chr2.3:50612550-50612569 MS.gene56161:intron 30.0%
! CCCTAAAATTAGCTTGATAT+GGG + chr2.3:50612553-50612572 None:intergenic 30.0%
! GCTAAATCAAGAGAAAGTTT+TGG + chr2.3:50611563-50611582 None:intergenic 30.0%
! TCCCTAAAATTAGCTTGATA+TGG + chr2.3:50612554-50612573 None:intergenic 30.0%
! TGGATCTAAATCCATGATTT+TGG + chr2.3:50611796-50611815 None:intergenic 30.0%
!! AAGCAACACTCATTTTGAAA+CGG - chr2.3:50612827-50612846 MS.gene56161:intron 30.0%
!! ATATAAGTCTTTGGTTTGCA+TGG + chr2.3:50613396-50613415 None:intergenic 30.0%
!!! AACTTTTTTTTTAGTGGTCG+TGG - chr2.3:50613014-50613033 MS.gene56161:intron 30.0%
!!! CGATATTGATTTTGTGATGT+TGG + chr2.3:50613897-50613916 None:intergenic 30.0%
!!! GACACTCATTTTAAAACTGA+GGG + chr2.3:50612588-50612607 None:intergenic 30.0%
AAACGAGAAATTCATGATGG+GGG + chr2.3:50613803-50613822 None:intergenic 35.0%
AATATTGAACCAGAATTGGG+AGG + chr2.3:50613605-50613624 None:intergenic 35.0%
ACTTATCATGTCTCCATTGA+AGG + chr2.3:50614683-50614702 None:intergenic 35.0%
AGAATAAAAGTCTATCGGAC+CGG + chr2.3:50613693-50613712 None:intergenic 35.0%
AGATGGATCATATGACAACT+AGG - chr2.3:50611444-50611463 MS.gene56161:CDS 35.0%
ATCATATGACAACTAGGAAG+AGG - chr2.3:50611450-50611469 MS.gene56161:CDS 35.0%
CTTCATTATCAACCCATGAA+TGG - chr2.3:50614736-50614755 MS.gene56161:CDS 35.0%
GGAAATTCTGACTCACATTT+AGG + chr2.3:50614390-50614409 None:intergenic 35.0%
GTGCAATTGTGCTAAAAACT+TGG - chr2.3:50614038-50614057 MS.gene56161:intron 35.0%
GTTGAGATTCAGTTCTTCTA+AGG + chr2.3:50614170-50614189 None:intergenic 35.0%
TCAACTTAAGGAATTACGAC+GGG + chr2.3:50614815-50614834 None:intergenic 35.0%
TCAATGGAGACATGATAAGT+GGG - chr2.3:50614683-50614702 MS.gene56161:CDS 35.0%
TCATATGACAACTAGGAAGA+GGG - chr2.3:50611451-50611470 MS.gene56161:CDS 35.0%
TCATCAGATGACAATGAAGA+TGG - chr2.3:50611427-50611446 MS.gene56161:CDS 35.0%
TCATGATGAGATCAATAGCT+AGG - chr2.3:50611751-50611770 MS.gene56161:CDS 35.0%
TGCATAACTATCTACCAAGA+CGG - chr2.3:50613077-50613096 MS.gene56161:intron 35.0%
TGGCATAATCTTGTACTACT+TGG - chr2.3:50611880-50611899 MS.gene56161:CDS 35.0%
TTCAATGGAGACATGATAAG+TGG - chr2.3:50614682-50614701 MS.gene56161:CDS 35.0%
!!! AACACTCATTTTGAAACGGA+GGG - chr2.3:50612831-50612850 MS.gene56161:intron 35.0%
!!! CGACACTCATTTTAAAACTG+AGG + chr2.3:50612589-50612608 None:intergenic 35.0%
!!! GTTTTTTTAAGTTCCCGTCT+TGG + chr2.3:50613094-50613113 None:intergenic 35.0%
AATCTTGCCGTTAATGACTC+CGG + chr2.3:50612297-50612316 None:intergenic 40.0%
ACAACCAATGGAATTGGGTA+GGG + chr2.3:50612364-50612383 None:intergenic 40.0%
ACCAAGACTTATACCAAGAG+GGG - chr2.3:50613048-50613067 MS.gene56161:intron 40.0%
AGAGAGACAACCAATGGAAT+TGG + chr2.3:50612370-50612389 None:intergenic 40.0%
AGCAAACTTCCAGACACAAT+AGG - chr2.3:50614202-50614221 MS.gene56161:intron 40.0%
AGGAAGACACACAAGTTTGT+TGG + chr2.3:50614266-50614285 None:intergenic 40.0%
ATATCCCAACCAAGAAGAGA+TGG - chr2.3:50614638-50614657 MS.gene56161:CDS 40.0%
ATCTCCAGGTATAATCTCCA+AGG - chr2.3:50611615-50611634 MS.gene56161:CDS 40.0%
CAACTTAAGGAATTACGACG+GGG + chr2.3:50614814-50614833 None:intergenic 40.0%
CATAAGAGTCATCATGTGAG+AGG + chr2.3:50614296-50614315 None:intergenic 40.0%
CTCTCACATGATGACTCTTA+TGG - chr2.3:50614294-50614313 MS.gene56161:intron 40.0%
CTCTTGGTATAAGTCTTGGT+CGG + chr2.3:50613048-50613067 None:intergenic 40.0%
GAACATACCGGAGTCATTAA+CGG - chr2.3:50612287-50612306 MS.gene56161:intron 40.0%
GACCAAGACTTATACCAAGA+GGG - chr2.3:50613047-50613066 MS.gene56161:intron 40.0%
GAGAGACAACCAATGGAATT+GGG + chr2.3:50612369-50612388 None:intergenic 40.0%
GAGTCAGAATTTCCGGTATT+TGG - chr2.3:50614396-50614415 MS.gene56161:intron 40.0%
GAGTTTAGAGAGACAACCAA+TGG + chr2.3:50612376-50612395 None:intergenic 40.0%
GCATAACTATCTACCAAGAC+GGG - chr2.3:50613078-50613097 MS.gene56161:intron 40.0%
GCATGCTTCACGTCTTTATA+AGG - chr2.3:50613976-50613995 MS.gene56161:intron 40.0%
GGAAGATAGAGTTCATGTTG+TGG - chr2.3:50611508-50611527 MS.gene56161:CDS 40.0%
GGGAAACGATCAGAACATAA+GGG - chr2.3:50614703-50614722 MS.gene56161:CDS 40.0%
TCAACCTTGGAGATTATACC+TGG + chr2.3:50611622-50611641 None:intergenic 40.0%
TCTTCTTGGTTGGGATATGA+TGG + chr2.3:50614636-50614655 None:intergenic 40.0%
TCTTGGTATAAGTCTTGGTC+GGG + chr2.3:50613047-50613066 None:intergenic 40.0%
TGAGATCAATAGCTAGGAAC+AGG - chr2.3:50611757-50611776 MS.gene56161:CDS 40.0%
TGCAATTCTCAATGGTTGCT+GGG + chr2.3:50612437-50612456 None:intergenic 40.0%
TGGGAAACGATCAGAACATA+AGG - chr2.3:50614702-50614721 MS.gene56161:CDS 40.0%
TTAGGGTTTCAAGATTGACG+AGG + chr2.3:50614372-50614391 None:intergenic 40.0%
! AGCTAACGTCTAGTTCAACT+AGG + chr2.3:50614456-50614475 None:intergenic 40.0%
! GCTAACGTCTAGTTCAACTA+GGG + chr2.3:50614455-50614474 None:intergenic 40.0%
! TCGGACCGGATTTTTAATCA+TGG + chr2.3:50613679-50613698 None:intergenic 40.0%
!! AATTGGGAGGTTTTGTCTGT+TGG + chr2.3:50613592-50613611 None:intergenic 40.0%
!! ATTCAGTTCTTCTAAGGCTC+TGG + chr2.3:50614164-50614183 None:intergenic 40.0%
!! CTCAAAGCTTTGAATGTCTC+TGG - chr2.3:50612393-50612412 MS.gene56161:intron 40.0%
!! CTTTGGTTTGCATGGTACAT+GGG + chr2.3:50613388-50613407 None:intergenic 40.0%
!! TCTTTGGTTTGCATGGTACA+TGG + chr2.3:50613389-50613408 None:intergenic 40.0%
!!! AAGTTTTGGAAGTGAGTCCA+AGG + chr2.3:50611549-50611568 None:intergenic 40.0%
!!! CAACACTCATTTTGAAACGG+AGG - chr2.3:50612830-50612849 MS.gene56161:intron 40.0%
AAAACATTGCCGGACTCCAT+CGG - chr2.3:50614487-50614506 MS.gene56161:intron 45.0%
AAGAGGAGTCCTATGGAGTA+GGG + chr2.3:50614430-50614449 None:intergenic 45.0%
AAGAGTCATCATGTGAGAGG+TGG + chr2.3:50614293-50614312 None:intergenic 45.0%
AATTCTCAATGGTTGCTGGG+AGG + chr2.3:50612434-50612453 None:intergenic 45.0%
ACGTACGTTTGAATTGCCTC+AGG - chr2.3:50614323-50614342 MS.gene56161:intron 45.0%
AGATCCCTACCCAATTCCAT+TGG - chr2.3:50612357-50612376 MS.gene56161:intron 45.0%
AGCTCAAAGCCTATTGTGTC+TGG + chr2.3:50614214-50614233 None:intergenic 45.0%
AGGGCAATGACTCCAAATAC+CGG + chr2.3:50614411-50614430 None:intergenic 45.0%
ATCCACCATCTCTTCTTGGT+TGG + chr2.3:50614646-50614665 None:intergenic 45.0%
ATTCTCAATGGTTGCTGGGA+GGG + chr2.3:50612433-50612452 None:intergenic 45.0%
CAAGATTGACGAGGTTCTCA+AGG + chr2.3:50614363-50614382 None:intergenic 45.0%
CAGACAAAACCTCCCAATTC+TGG - chr2.3:50613593-50613612 MS.gene56161:intron 45.0%
CAGCAACCATTGAGAATTGC+AGG - chr2.3:50612436-50612455 MS.gene56161:intron 45.0%
CGACCAAGACTTATACCAAG+AGG - chr2.3:50613046-50613065 MS.gene56161:intron 45.0%
CTAGTTCAACTAGGGACAAG+AGG + chr2.3:50614447-50614466 None:intergenic 45.0%
CTGCAATTCTCAATGGTTGC+TGG + chr2.3:50612438-50612457 None:intergenic 45.0%
CTTGGTATAAGTCTTGGTCG+GGG + chr2.3:50613046-50613065 None:intergenic 45.0%
GACAACCAATGGAATTGGGT+AGG + chr2.3:50612365-50612384 None:intergenic 45.0%
GCACTAGAGAGCCAAAATCA+TGG - chr2.3:50611782-50611801 MS.gene56161:CDS 45.0%
GGTGAAGCAAGACCATTCAT+GGG + chr2.3:50614751-50614770 None:intergenic 45.0%
GTGAAATGTGGGACCTTCAA+TGG - chr2.3:50614667-50614686 MS.gene56161:CDS 45.0%
GTTCATGTTGTGGACTTGCA+TGG - chr2.3:50611518-50611537 MS.gene56161:CDS 45.0%
TCCACCATCTCTTCTTGGTT+GGG + chr2.3:50614645-50614664 None:intergenic 45.0%
TCCCCTCTTGGTATAAGTCT+TGG + chr2.3:50613052-50613071 None:intergenic 45.0%
TGAACTCTATCTTCCTCCGT+CGG + chr2.3:50611503-50611522 None:intergenic 45.0%
TGGTGAAGCAAGACCATTCA+TGG + chr2.3:50614752-50614771 None:intergenic 45.0%
TTATGCAAAACGTCCCCTCT+TGG + chr2.3:50613064-50613083 None:intergenic 45.0%
! ATGAATGGTCTTGCTTCACC+AGG - chr2.3:50614751-50614770 MS.gene56161:CDS 45.0%
!! AACCAAGAAGAGATGGTGGA+TGG - chr2.3:50614645-50614664 MS.gene56161:CDS 45.0%
!! AGATGGTGGATGGTGAAATG+TGG - chr2.3:50614655-50614674 MS.gene56161:CDS 45.0%
!! GATGGTGGATGGTGAAATGT+GGG - chr2.3:50614656-50614675 MS.gene56161:CDS 45.0%
!!! TTAGCTTTTGGAGCTTCTGG+AGG + chr2.3:50614516-50614535 None:intergenic 45.0%
!!! TTTATTAATATTTTAAAAAC+TGG - chr2.3:50613557-50613576 MS.gene56161:intron 5.0%
AATTACGACGGGGTGAGAAG+AGG + chr2.3:50614804-50614823 None:intergenic 50.0%
AATTGGGTAGGGATCTGAGC+TGG + chr2.3:50612353-50612372 None:intergenic 50.0%
ATCATGTGAGAGGTGGAGCT+AGG + chr2.3:50614286-50614305 None:intergenic 50.0%
CAAGAGGAGTCCTATGGAGT+AGG + chr2.3:50614431-50614450 None:intergenic 50.0%
CACCATCCACCATCTCTTCT+TGG + chr2.3:50614650-50614669 None:intergenic 50.0%
CTCCAAAAGCTAAGCGTGGA+GGG - chr2.3:50614523-50614542 MS.gene56161:intron 50.0%
CTTGCTTCACCAGGCTTCAT+GGG - chr2.3:50614760-50614779 MS.gene56161:CDS 50.0%
GAAGCTCCAAAAGCTAAGCG+TGG - chr2.3:50614519-50614538 MS.gene56161:intron 50.0%
GAGTCATTGCCCTACTCCAT+AGG - chr2.3:50614418-50614437 MS.gene56161:intron 50.0%
TAGGGACAAGAGGAGTCCTA+TGG + chr2.3:50614437-50614456 None:intergenic 50.0%
TCCCAACCAAGAAGAGATGG+TGG - chr2.3:50614641-50614660 MS.gene56161:CDS 50.0%
TCTTGCTTCACCAGGCTTCA+TGG - chr2.3:50614759-50614778 MS.gene56161:CDS 50.0%
TTGCTTCACCAGGCTTCATG+GGG - chr2.3:50614761-50614780 MS.gene56161:CDS 50.0%
!! CAGTTCTTCTAAGGCTCTGG+TGG + chr2.3:50614161-50614180 None:intergenic 50.0%
!! TTCCCTCCACGCTTAGCTTT+TGG + chr2.3:50614528-50614547 None:intergenic 50.0%
!!! CGCTTAGCTTTTGGAGCTTC+TGG + chr2.3:50614519-50614538 None:intergenic 50.0%
ACGAGGTTCTCAAGGTCGTC+GGG + chr2.3:50614355-50614374 None:intergenic 55.0%
CAATGGTTGCTGGGAGGGAT+TGG + chr2.3:50612428-50612447 None:intergenic 55.0%
GACGAGGTTCTCAAGGTCGT+CGG + chr2.3:50614356-50614375 None:intergenic 55.0%
GCTCCAAAAGCTAAGCGTGG+AGG - chr2.3:50614522-50614541 MS.gene56161:intron 55.0%
GGACTTGCATGGCATGTCCT+TGG - chr2.3:50611529-50611548 MS.gene56161:CDS 55.0%
CGAGGTTCTCAAGGTCGTCG+GGG + chr2.3:50614354-50614373 None:intergenic 60.0%
CTATCTTCCTCCGTCGGCGT+CGG + chr2.3:50611497-50611516 None:intergenic 60.0%
CTTCACCAGGCTTCATGGGG+AGG - chr2.3:50614764-50614783 MS.gene56161:CDS 60.0%
GACAACCTCCCCATGAAGCC+TGG + chr2.3:50614772-50614791 None:intergenic 60.0%
GGGTAGGGATCTGAGCTGGT+TGG + chr2.3:50612349-50612368 None:intergenic 60.0%
GGTGGAGCAGGGATTGCATG+TGG - chr2.3:50614576-50614595 MS.gene56161:intron 60.0%
TCCAGTGGAGGCGAACTGAG+CGG + chr2.3:50614553-50614572 None:intergenic 60.0%
!! GAGCTTCTGGAGGCATCCGA+TGG + chr2.3:50614506-50614525 None:intergenic 60.0%
!! TGGAGGCATCCGATGGAGTC+CGG + chr2.3:50614499-50614518 None:intergenic 60.0%
CAGTTCGCCTCCACTGGAGG+TGG - chr2.3:50614555-50614574 MS.gene56161:intron 65.0%
CCAGTGGAGGCGAACTGAGC+GGG + chr2.3:50614552-50614571 None:intergenic 65.0%
CGGGGTGAGAAGAGGCGAAG+AGG + chr2.3:50614796-50614815 None:intergenic 65.0%
GCTCAGTTCGCCTCCACTGG+AGG - chr2.3:50614552-50614571 MS.gene56161:intron 65.0%
TGCTCCACCACCTCCAGTGG+AGG + chr2.3:50614565-50614584 None:intergenic 65.0%
TTCGCCTCCACTGGAGGTGG+TGG - chr2.3:50614558-50614577 MS.gene56161:intron 65.0%
!! CCACTGGAGGTGGTGGAGCA+GGG - chr2.3:50614565-50614584 MS.gene56161:intron 65.0%
!! TCCACTGGAGGTGGTGGAGC+AGG - chr2.3:50614564-50614583 MS.gene56161:intron 65.0%
CCCGCTCAGTTCGCCTCCAC+TGG - chr2.3:50614549-50614568 MS.gene56161:intron 70.0%
CCCTGCTCCACCACCTCCAG+TGG + chr2.3:50614568-50614587 None:intergenic 70.0%
GGTCGTCGGGGAGACTCCTG+AGG + chr2.3:50614342-50614361 None:intergenic 70.0%
! TCTTCCTCCGTCGGCGTCGG+CGG + chr2.3:50611494-50611513 None:intergenic 70.0%
GAAGACGCCGCCGACGCCGA+CGG - chr2.3:50611484-50611503 MS.gene56161:CDS 75.0%
! GACGCCGCCGACGCCGACGG+AGG - chr2.3:50611487-50611506 MS.gene56161:CDS 85.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 50611389 50614834 50611389 ID=MS.gene56161
chr2.3 mRNA 50611389 50614834 50611389 ID=MS.gene56161.t1;Parent=MS.gene56161
chr2.3 exon 50614601 50614834 50614601 ID=MS.gene56161.t1.exon1;Parent=MS.gene56161.t1
chr2.3 CDS 50614601 50614834 50614601 ID=cds.MS.gene56161.t1;Parent=MS.gene56161.t1
chr2.3 exon 50613766 50613935 50613766 ID=MS.gene56161.t1.exon2;Parent=MS.gene56161.t1
chr2.3 CDS 50613766 50613935 50613766 ID=cds.MS.gene56161.t1;Parent=MS.gene56161.t1
chr2.3 exon 50611389 50612058 50611389 ID=MS.gene56161.t1.exon3;Parent=MS.gene56161.t1
chr2.3 CDS 50611389 50612058 50611389 ID=cds.MS.gene56161.t1;Parent=MS.gene56161.t1
Gene Sequence

>MS.gene56161

ATGTATGAATTGCAACAATTTCAGTTTCTTCATCAAGATCATCAGATGACAATGAAGATGGATCATATGACAACTAGGAAGAGGGAGACAAAAAAGAAGACGCCGCCGACGCCGACGGAGGAAGATAGAGTTCATGTTGTGGACTTGCATGGCATGTCCTTGGACTCACTTCCAAAACTTTCTCTTGATTTAGCTATTATTTCCAAGCTCAATTTGTCTAACAATAATCTCCAGGTATAATCTCCAAGGTTGACAAATTCACAATTAATTTAGTACAATGCAATTTTATTAAGGTTTTGAAGTTTAGGTTTTTTCATCAAAATTGTTGTACAATCACAATTATTAATATATATGATTGACACTCATGATGAGATCAATAGCTAGGAACAGGATGCACTAGAGAGCCAAAATCATGGATTTAGATCCAAATCTTAAAATCATAGTGGTTTAGCTGTCCATAACAAATAGAATAATCTTTGTTGAAGGGAAAATGGCATAATCTTGTACTACTTGGATTTTTTTGATAAGTATCTGGTGCAACTAGTATTAGTATATATTCATTTTTTTTGTACATATTCATTTTATTAAGTCTAAAGAATATATTTGATTAAGACATACTTAACTTAATATATTAGATGTAATATAACGGTTAGCTTTGATACAGATATCTTCTTCTAAATTGATTGAATATCTTCTTCTTTTTTTAATGAACATGAACATTTATTTTTATGAACAATTAATTGAATATCTTTTTTTTAATGAACAATTGATTGAATATTTGAATATCTTTTTTTTTAATGAACAATTGATTGAATATCTTTTATTTTATGTAAAATTGATTGAATATCTTGTTATATAAATTATTTGATAGGAAATAACTATTTAACTATATATAACAGAACATACCGGAGTCATTAACGGCAAGATTATTAAACTTGGAGATGTTGGACGTGCACTCCAACCAGCTCAGATCCCTACCCAATTCCATTGGTTGTCTCTCTAAACTCAAAGCTTTGAATGTCTCTGGAAACTTCATCCAATCCCTCCCAGCAACCATTGAGAATTGCAGGTTTATTTTCTTAACTTCTCTTTATATTTTATCGATAATTAAATATATGATAAAATTATCTCATATTATAACTTTTTGAATTTTAAGTGTTACCCATATCAAGCTAATTTTAGGGATTTATTTTACTCCCTCAGTTTTAAAATGAGTGTCGTTTTAGGTGTTTACACACATATTAAGAAATGCAATGATTAAAAGAAAGAGAATGATTATTTTACCAAATTATCCTTATTAACTATTGGTTAGTTTTAAAAGTAATACTTTGGAAGTAGTGTGCATAGTATTAATTAGAGGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGGAAACTGAAAGTGACATTCATTTTGAAACAAATTTTTTTGGCTAAAGCAACACTCATTTTGAAACGGAGGGAGTACTAAGTTTGACAACTGATGTCAAAAGATGCGACTCATATACATGTTTTTAGTTGTGTATAGGTGTTATTACTTTCTTTCAGCATACATATGACTTTTCAAATTTTGAATGCTGACATTATTACAAGAACATCCATGTTCATTATTGTGTTTTGCATAACTTTTTTTTTAGTGGTCGTGGTTTGAACCCCGACCAAGACTTATACCAAGAGGGGACGTTTTGCATAACTATCTACCAAGACGGGAACTTAAAAAAACAAATAAATATTTAATTGATTTGAGAATCTATAATTTTTTTAATTTTTTTTGTATTTTTTCATAAATTTATTAAGATCTAGAAAATAGATTAATTATATCTCATCAAGCTAATCAAATTTTTGTTTACAAGAGTAATTGCTATTACTTTTCAAAAGAAAAGACTAATTGCTATATATTAGTTAACAACTCAAACAAGTTATCTTTTTTTTCTTGTGATACCTCAAACAAGTTATCTAATCATCTCTAATTAAACATAATCATATTTAATTTTCCCATGTACCATGCAAACCAAAGACTTATATTAACAAATTACATTTGTAGGTGTCTAATCATATTTCAACAAATATCTAGTATTCACATTTTACTTTCACCTAATGATGTCATGCAAATAAAAAAAATAACAAAAAGATCGAGAAATTGACTTTGATGTAAATTGATATTTTTTTATTAATATTTTAAAAACTGGATTGTAGATCCAACAGACAAAACCTCCCAATTCTGGTTCAATATTTGTTTAACAAAATAAAAAAATATTATTAACAAAATAAACTGTTTGAACTAACCATGATTAAAAATCCGGTCCGATAGACTTTTATTCTTGTTTTTTTTTAGGGGAGACTTTTTTTTTTGAAAGAATTTTTAGGGGAGACTTTGTATACTTGTTTTGCAATGATATAATATAAATACCCCCATCATGAATTTCTCGTTTTTAATTGTCTCTCTCTTAAAAAATATATTTAATTACCTCAAATTTTCAATTCTAATCAAGAATTTCTAGATCCAACATCACAAAATCAATATCGTAAACTCTACGCATATTATATCTCTAAACCTACCAATTAGATATTGATTGAATAATGCTGCATGCTTCACGTCTTTATAAGGCTCACATCTATGTCACCATTATTATATGATAAAATTGGAGTGCAATTGTGCTAAAAACTTGGTTATGTGTGTTACTGTTACATATCATTGTTAATATTGATTATTACATGTTTTTTTCATTCTTTTTTCTAACATGCACATGTGCATATGAATTTTGATCCACCAGAGCCTTAGAAGAACTGAATCTCAACTTCAACATGTTGAGCAAACTTCCAGACACAATAGGCTTTGAGCTCGTAAAACTCAAGAAGCTTGCAGTTAACTCCAACAAACTTGTGTGTCTTCCTAGCTCCACCTCTCACATGATGACTCTTATGGTTCTCGACGTACGTTTGAATTGCCTCAGGAGTCTCCCCGACGACCTTGAGAACCTCGTCAATCTTGAAACCCTAAATGTGAGTCAGAATTTCCGGTATTTGGAGTCATTGCCCTACTCCATAGGACTCCTCTTGTCCCTAGTTGAACTAGACGTTAGCTACAACAATATCAAAACATTGCCGGACTCCATCGGATGCCTCCAGAAGCTCCAAAAGCTAAGCGTGGAGGGAAACCCGCTCAGTTCGCCTCCACTGGAGGTGGTGGAGCAGGGATTGCATGTGGTGAAAGAGTATATGTGTAACAAAATGAACTCAAGCCATCATATCCCAACCAAGAAGAGATGGTGGATGGTGAAATGTGGGACCTTCAATGGAGACATGATAAGTGGGAAACGATCAGAACATAAGGGTTTCAACAAGCTTCATTATCAACCCATGAATGGTCTTGCTTCACCAGGCTTCATGGGGAGGTTGTCGCCTCTTCGCCTCTTCTCACCCCGTCGTAATTCCTTAAGTTGA
Protein sequence

>MS.gene56161.t1

MYELQQFQFLHQDHQMTMKMDHMTTRKRETKKKTPPTPTEEDRVHVVDLHGMSLDSLPKLSLDLAIISKLNLSNNNLQNIPESLTARLLNLEMLDVHSNQLRSLPNSIGCLSKLKALNVSGNFIQSLPATIENCRALEELNLNFNMLSKLPDTIGFELVKLKKLAVNSNKLVCLPSSTSHMMTLMVLDVRLNCLRSLPDDLENLVNLETLNVSQNFRYLESLPYSIGLLLSLVELDVSYNNIKTLPDSIGCLQKLQKLSVEGNPLSSPPLEVVEQGLHVVKEYMCNKMNSSHHIPTKKRWWMVKCGTFNGDMISGKRSEHKGFNKLHYQPMNGLASPGFMGRLSPLRLFSPRRNSLS