Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57519.t1 | XP_013444912.1 | 96.6 | 179 | 5 | 1 | 1 | 178 | 1 | 179 | 1.70E-84 | 322 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57519.t1 | Q58FX0 | 42.1 | 145 | 77 | 3 | 3 | 143 | 8 | 149 | 5.5e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57519.t1 | A0A072TNG6 | 96.6 | 179 | 5 | 1 | 1 | 178 | 1 | 179 | 1.2e-84 | 322.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene57519 | 0.873773 | 1.13E-67 | -1.69E-46 |
| MS.gene051226 | MS.gene57519 | 0.823631 | 1.25E-53 | -1.69E-46 |
| MS.gene052016 | MS.gene57519 | 0.82589 | 3.66E-54 | -1.69E-46 |
| MS.gene052493 | MS.gene57519 | 0.802246 | 6.14E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene57519 | 0.82652 | 2.59E-54 | -1.69E-46 |
| MS.gene053298 | MS.gene57519 | 0.800539 | 1.37E-48 | -1.69E-46 |
| MS.gene054178 | MS.gene57519 | 0.816355 | 5.77E-52 | -1.69E-46 |
| MS.gene054617 | MS.gene57519 | 0.803872 | 2.83E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene57519 | 0.854599 | 1.11E-61 | -1.69E-46 |
| MS.gene054893 | MS.gene57519 | 0.853434 | 2.40E-61 | -1.69E-46 |
| MS.gene056082 | MS.gene57519 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene57519 | 0.81902 | 1.45E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene57519 | -0.812447 | 4.22E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene57519 | 0.819664 | 1.03E-52 | -1.69E-46 |
| MS.gene060259 | MS.gene57519 | 0.844919 | 5.61E-59 | -1.69E-46 |
| MS.gene061047 | MS.gene57519 | 0.815037 | 1.14E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57519.t1 | MTR_8g432400 | 96.648 | 179 | 5 | 1 | 1 | 178 | 1 | 179 | 1.55e-126 | 353 |
| MS.gene57519.t1 | MTR_8g432390 | 92.179 | 179 | 13 | 1 | 1 | 178 | 1 | 179 | 3.48e-109 | 309 |
| MS.gene57519.t1 | MTR_8g432370 | 89.888 | 178 | 18 | 0 | 1 | 178 | 1 | 178 | 7.82e-108 | 306 |
| MS.gene57519.t1 | MTR_8g432350 | 89.385 | 179 | 18 | 1 | 1 | 178 | 1 | 179 | 3.68e-106 | 301 |
| MS.gene57519.t1 | MTR_7g076000 | 54.396 | 182 | 62 | 6 | 1 | 177 | 1 | 166 | 2.19e-56 | 175 |
| MS.gene57519.t1 | MTR_8g432470 | 39.891 | 183 | 93 | 5 | 2 | 174 | 5 | 180 | 5.96e-31 | 111 |
| MS.gene57519.t1 | MTR_8g432450 | 38.418 | 177 | 94 | 3 | 2 | 168 | 5 | 176 | 1.83e-29 | 107 |
| MS.gene57519.t1 | MTR_8g432440 | 38.251 | 183 | 96 | 6 | 2 | 174 | 5 | 180 | 1.72e-28 | 104 |
| MS.gene57519.t1 | MTR_8g432420 | 38.251 | 183 | 96 | 6 | 2 | 174 | 5 | 180 | 1.72e-28 | 104 |
| MS.gene57519.t1 | MTR_8g432460 | 37.363 | 182 | 98 | 5 | 2 | 174 | 5 | 179 | 8.55e-27 | 100 |
| MS.gene57519.t1 | MTR_8g432490 | 36.813 | 182 | 99 | 5 | 2 | 174 | 5 | 179 | 3.17e-26 | 99.0 |
| MS.gene57519.t1 | MTR_3g064780 | 36.813 | 182 | 99 | 6 | 2 | 174 | 5 | 179 | 3.89e-24 | 95.1 |
| MS.gene57519.t1 | MTR_8g432480 | 33.529 | 170 | 104 | 4 | 2 | 170 | 7 | 168 | 4.09e-22 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57519.t1 | AT2G45760 | 35.784 | 204 | 99 | 5 | 1 | 176 | 6 | 205 | 4.57e-35 | 122 |
| MS.gene57519.t1 | AT3G61190 | 38.798 | 183 | 101 | 6 | 1 | 178 | 16 | 192 | 7.36e-29 | 105 |
| MS.gene57519.t1 | AT3G61190 | 38.798 | 183 | 101 | 6 | 1 | 178 | 16 | 192 | 7.36e-29 | 105 |
Find 41 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAATTTCATATGTTTCTT+TGG | 0.263645 | 8.3:+61900144 | None:intergenic |
| GTGAGTGGAATTCCGGTAAC+TGG | 0.321207 | 8.3:-61900236 | MS.gene57519:CDS |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8.3:-61900328 | MS.gene57519:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8.3:-61900329 | MS.gene57519:CDS |
| TAACAGGAATTCCAGCTGTT+TGG | 0.411065 | 8.3:-61900175 | MS.gene57519:CDS |
| TCATGCAGCGGAGTTGTAAC+AGG | 0.413933 | 8.3:-61900191 | MS.gene57519:CDS |
| GTCGACGGTGAGTGGAATTC+CGG | 0.416179 | 8.3:-61900243 | MS.gene57519:CDS |
| CACATACCCTCCAAGAAAAT+CGG | 0.422233 | 8.3:+61900391 | None:intergenic |
| TCCGCTGCATGAGCCATTAC+CGG | 0.425688 | 8.3:+61900202 | None:intergenic |
| GGGGTTCCGGTGACCGGTAA+TGG | 0.436757 | 8.3:-61900215 | MS.gene57519:CDS |
| AATTCCGGTAACTGGGGTTC+CGG | 0.438542 | 8.3:-61900228 | MS.gene57519:CDS |
| CCTCCAAGAAAATCGGAAAC+AGG | 0.459437 | 8.3:+61900398 | None:intergenic |
| GTCACCGGAACCCCAGTTAC+CGG | 0.462256 | 8.3:+61900224 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.3:-61900337 | MS.gene57519:CDS |
| TGGTAGTTTAACCAAACAGC+TGG | 0.466544 | 8.3:+61900164 | None:intergenic |
| ACCTTCGGAATCAAGTTTCG+TGG | 0.478987 | 8.3:+61900544 | None:intergenic |
| GGTTGTAAATATTTCTGTAA+AGG | 0.481719 | 8.3:-61900306 | MS.gene57519:CDS |
| GTTATGAACCTTGCATGTAA+TGG | 0.492338 | 8.3:+61900479 | None:intergenic |
| GTAACTGGGGTTCCGGTGAC+CGG | 0.498566 | 8.3:-61900221 | MS.gene57519:CDS |
| AGAAGTAGTAGTAATAGTGT+TGG | 0.499927 | 8.3:-61900434 | MS.gene57519:CDS |
| CCAACTTCTCGTTCCATGTA+GGG | 0.504708 | 8.3:+61900513 | None:intergenic |
| TGCAAGGTTCATAACAATAG+AGG | 0.521084 | 8.3:-61900471 | MS.gene57519:CDS |
| TGAGTGGAATTCCGGTAACT+GGG | 0.521789 | 8.3:-61900235 | MS.gene57519:CDS |
| TGTAGGGTAACTTCCACCTT+CGG | 0.525270 | 8.3:+61900529 | None:intergenic |
| TCTATGTCGTCGACGGTGAG+TGG | 0.526798 | 8.3:-61900251 | MS.gene57519:CDS |
| TTCGTCACGGTTCAATGTGA+TGG | 0.531098 | 8.3:-61900587 | MS.gene57519:CDS |
| TAGTAGTAATAGTGTTGGAA+TGG | 0.545051 | 8.3:-61900429 | MS.gene57519:CDS |
| AAGGTGGAAGTTACCCTACA+TGG | 0.553497 | 8.3:-61900526 | MS.gene57519:CDS |
| CCCTACATGGAACGAGAAGT+TGG | 0.557629 | 8.3:-61900513 | MS.gene57519:CDS |
| ACGAAACTTGATTCCGAAGG+TGG | 0.571494 | 8.3:-61900542 | MS.gene57519:CDS |
| ACCGGTAATGGCTCATGCAG+CGG | 0.575607 | 8.3:-61900203 | MS.gene57519:CDS |
| CATGAGCCATTACCGGTCAC+CGG | 0.578927 | 8.3:+61900209 | None:intergenic |
| CTCGAGTTCTATGTCGTCGA+CGG | 0.597662 | 8.3:-61900258 | MS.gene57519:CDS |
| ACCAACTTCTCGTTCCATGT+AGG | 0.602619 | 8.3:+61900512 | None:intergenic |
| TCGAGGTGCCATTACATGCA+AGG | 0.628784 | 8.3:-61900487 | MS.gene57519:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8.3:-61900327 | MS.gene57519:CDS |
| GAACGAGAAGTTGGTGATCG+AGG | 0.646266 | 8.3:-61900504 | MS.gene57519:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.658333 | 8.3:-61900545 | MS.gene57519:CDS |
| GAGTGGAATTCCGGTAACTG+GGG | 0.673539 | 8.3:-61900234 | MS.gene57519:CDS |
| AAAAGGGAACACATTCGTCA+CGG | 0.677311 | 8.3:-61900600 | MS.gene57519:CDS |
| TCAATGTGATGGAAGCAATG+AGG | 0.684597 | 8.3:-61900576 | MS.gene57519:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.3:61900458-61900477 | MS.gene57519:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.3:61900464-61900483 | MS.gene57519:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.3:61900465-61900484 | MS.gene57519:CDS | 20.0% |
| ! | AGAACAAAAAAGCCATAAAA+GGG | - | chr8.3:61900200-61900219 | MS.gene57519:CDS | 25.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | - | chr8.3:61900488-61900507 | MS.gene57519:CDS | 25.0% |
| !! | GGTTGTAAATATTTCTGTAA+AGG | - | chr8.3:61900510-61900529 | MS.gene57519:CDS | 25.0% |
| AGAAGTAGTAGTAATAGTGT+TGG | - | chr8.3:61900382-61900401 | MS.gene57519:CDS | 30.0% | |
| GAGAACAAAAAAGCCATAAA+AGG | - | chr8.3:61900199-61900218 | MS.gene57519:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+TGG | - | chr8.3:61900487-61900506 | MS.gene57519:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | - | chr8.3:61900489-61900508 | MS.gene57519:CDS | 30.0% | |
| ! | TAGTAGTAATAGTGTTGGAA+TGG | - | chr8.3:61900387-61900406 | MS.gene57519:CDS | 30.0% |
| GTTATGAACCTTGCATGTAA+TGG | + | chr8.3:61900340-61900359 | None:intergenic | 35.0% | |
| TGCAAGGTTCATAACAATAG+AGG | - | chr8.3:61900345-61900364 | MS.gene57519:CDS | 35.0% | |
| ! | AACTCGAGCAAGAATTTTGT+TGG | + | chr8.3:61900546-61900565 | None:intergenic | 35.0% |
| ! | ACTCGAGCAAGAATTTTGTT+GGG | + | chr8.3:61900545-61900564 | None:intergenic | 35.0% |
| ! | ATACCTGTTTCCGATTTTCT+TGG | - | chr8.3:61900415-61900434 | MS.gene57519:CDS | 35.0% |
| AAAAGGGAACACATTCGTCA+CGG | - | chr8.3:61900216-61900235 | MS.gene57519:CDS | 40.0% | |
| CACATACCCTCCAAGAAAAT+CGG | + | chr8.3:61900428-61900447 | None:intergenic | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.3:61900479-61900498 | MS.gene57519:CDS | 40.0% | |
| TAACAGGAATTCCAGCTGTT+TGG | - | chr8.3:61900641-61900660 | MS.gene57519:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+TGG | + | chr8.3:61900655-61900674 | None:intergenic | 40.0% | |
| ! | CTGTTTCCGATTTTCTTGGA+GGG | - | chr8.3:61900419-61900438 | MS.gene57519:CDS | 40.0% |
| ! | GACGAATGTGTTCCCTTTTA+TGG | + | chr8.3:61900215-61900234 | None:intergenic | 40.0% |
| ! | TCAATGTGATGGAAGCAATG+AGG | - | chr8.3:61900240-61900259 | MS.gene57519:CDS | 40.0% |
| AAGGTGGAAGTTACCCTACA+TGG | - | chr8.3:61900290-61900309 | MS.gene57519:CDS | 45.0% | |
| ACCAACTTCTCGTTCCATGT+AGG | + | chr8.3:61900307-61900326 | None:intergenic | 45.0% | |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.3:61900271-61900290 | MS.gene57519:CDS | 45.0% | |
| ACCTTCGGAATCAAGTTTCG+TGG | + | chr8.3:61900275-61900294 | None:intergenic | 45.0% | |
| ACGAAACTTGATTCCGAAGG+TGG | - | chr8.3:61900274-61900293 | MS.gene57519:CDS | 45.0% | |
| CCAACTTCTCGTTCCATGTA+GGG | + | chr8.3:61900306-61900325 | None:intergenic | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+AGG | + | chr8.3:61900421-61900440 | None:intergenic | 45.0% | |
| TGAGTGGAATTCCGGTAACT+GGG | - | chr8.3:61900581-61900600 | MS.gene57519:CDS | 45.0% | |
| TGTAGGGTAACTTCCACCTT+CGG | + | chr8.3:61900290-61900309 | None:intergenic | 45.0% | |
| TTCGTCACGGTTCAATGTGA+TGG | - | chr8.3:61900229-61900248 | MS.gene57519:CDS | 45.0% | |
| ! | CCTGTTTCCGATTTTCTTGG+AGG | - | chr8.3:61900418-61900437 | MS.gene57519:CDS | 45.0% |
| AATTCCGGTAACTGGGGTTC+CGG | - | chr8.3:61900588-61900607 | MS.gene57519:CDS | 50.0% | |
| CCCTACATGGAACGAGAAGT+TGG | - | chr8.3:61900303-61900322 | MS.gene57519:CDS | 50.0% | |
| CTCGAGTTCTATGTCGTCGA+CGG | - | chr8.3:61900558-61900577 | MS.gene57519:CDS | 50.0% | |
| GAGTGGAATTCCGGTAACTG+GGG | - | chr8.3:61900582-61900601 | MS.gene57519:CDS | 50.0% | |
| GTGAGTGGAATTCCGGTAAC+TGG | - | chr8.3:61900580-61900599 | MS.gene57519:CDS | 50.0% | |
| TCATGCAGCGGAGTTGTAAC+AGG | - | chr8.3:61900625-61900644 | MS.gene57519:CDS | 50.0% | |
| !! | GAACGAGAAGTTGGTGATCG+AGG | - | chr8.3:61900312-61900331 | MS.gene57519:CDS | 50.0% |
| !! | TCGAGGTGCCATTACATGCA+AGG | - | chr8.3:61900329-61900348 | MS.gene57519:CDS | 50.0% |
| CATGAGCCATTACCGGTCAC+CGG | + | chr8.3:61900610-61900629 | None:intergenic | 55.0% | |
| GTCGACGGTGAGTGGAATTC+CGG | - | chr8.3:61900573-61900592 | MS.gene57519:CDS | 55.0% | |
| TCCGCTGCATGAGCCATTAC+CGG | + | chr8.3:61900617-61900636 | None:intergenic | 55.0% | |
| TCTATGTCGTCGACGGTGAG+TGG | - | chr8.3:61900565-61900584 | MS.gene57519:CDS | 55.0% | |
| ! | ACCGGTAATGGCTCATGCAG+CGG | - | chr8.3:61900613-61900632 | MS.gene57519:CDS | 55.0% |
| GTAACTGGGGTTCCGGTGAC+CGG | - | chr8.3:61900595-61900614 | MS.gene57519:CDS | 60.0% | |
| GTCACCGGAACCCCAGTTAC+CGG | + | chr8.3:61900595-61900614 | None:intergenic | 60.0% | |
| GGGGTTCCGGTGACCGGTAA+TGG | - | chr8.3:61900601-61900620 | MS.gene57519:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61900151 | 61900687 | 61900151 | ID=MS.gene57519 |
| chr8.3 | mRNA | 61900151 | 61900687 | 61900151 | ID=MS.gene57519.t1;Parent=MS.gene57519 |
| chr8.3 | exon | 61900151 | 61900687 | 61900151 | ID=MS.gene57519.t1.exon1;Parent=MS.gene57519.t1 |
| chr8.3 | CDS | 61900151 | 61900687 | 61900151 | ID=cds.MS.gene57519.t1;Parent=MS.gene57519.t1 |
| Gene Sequence |
| Protein sequence |
