AlfalfaGEDB | MS.gene57710

M. sativa cultivar XinJiangDaYe / MS.gene57710

KEGG: K17086
GO: GO:0005575 GO:0016021 GO:0031224 GO:0110165
PFAM: PF02990.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57710.t1	RHN64084.1	99.7	656	2	0	1	656	1	656	0.00E+00	1302.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57710.t1	Q9FYQ8	84.5	658	100	2	1	656	1	658	0.0e+00	1136.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57710.t1	A0A396IID5	99.7	656	2	0	1	656	1	656	0.0e+00	1302.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050818	MS.gene57710	0.803294	3.73E-49	-1.69E-46
MS.gene057195	MS.gene57710	0.852472	4.51E-61	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene36542	MS.gene57710	PPI
MS.gene014910	MS.gene57710	PPI
MS.gene33117	MS.gene57710	PPI
MS.gene058486	MS.gene57710	PPI
MS.gene57710	MS.gene42664	PPI
MS.gene80184	MS.gene57710	PPI
MS.gene32702	MS.gene57710	PPI
MS.gene57710	MS.gene80181	PPI
MS.gene57710	MS.gene36992	PPI
MS.gene57710	MS.gene020254	PPI
MS.gene36990	MS.gene57710	PPI
MS.gene051485	MS.gene57710	PPI
MS.gene016086	MS.gene57710	PPI
MS.gene011190	MS.gene57710	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57710.t1	MTR_0204s0010	100.000	448	0	0	209	656	1	448	0.0	905
MS.gene57710.t1	MTR_3g082140	67.530	656	201	7	7	656	10	659	0.0	882
MS.gene57710.t1	MTR_5g098730	67.085	638	201	7	23	656	33	665	0.0	872
MS.gene57710.t1	MTR_8g016270	44.228	667	313	12	10	656	21	648	0.0	560
MS.gene57710.t1	MTR_1g014080	43.891	663	314	12	14	656	13	637	0.0	543
MS.gene57710.t1	MTR_3g105950	44.697	660	302	13	19	656	18	636	0.0	543
MS.gene57710.t1	MTR_4g116770	92.754	207	13	1	1	207	1	205	3.04e-130	400
MS.gene57710.t1	MTR_5g077170	27.673	636	378	16	32	656	27	591	5.67e-79	262
MS.gene57710.t1	MTR_6g074910	27.969	640	375	17	29	656	23	588	5.58e-78	259
MS.gene57710.t1	MTR_4g053270	28.527	638	366	15	32	656	29	589	2.93e-71	242
MS.gene57710.t1	MTR_8g088730	36.905	420	247	9	247	656	178	589	1.18e-66	229
MS.gene57710.t1	MTR_2g006300	36.930	417	245	9	250	656	175	583	2.97e-63	220

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57710.t1	AT5G35160	85.258	658	95	2	1	656	1	658	0.0	1149
MS.gene57710.t1	AT5G35160	85.258	658	95	2	1	656	1	658	0.0	1149
MS.gene57710.t1	AT5G35160	85.258	658	95	2	1	656	1	658	0.0	1149
MS.gene57710.t1	AT5G35160	81.763	658	90	3	1	656	1	630	0.0	1083
MS.gene57710.t1	AT4G12650	67.593	648	199	6	14	656	11	652	0.0	862
MS.gene57710.t1	AT3G13772	44.495	654	304	13	23	656	27	641	0.0	555
MS.gene57710.t1	AT5G10840	43.797	669	312	13	13	656	19	648	0.0	546
MS.gene57710.t1	AT5G25100	43.498	669	310	13	14	656	18	644	0.0	542
MS.gene57710.t1	AT2G24170	44.122	655	307	12	22	656	22	637	0.0	540
MS.gene57710.t1	AT5G25100	43.343	676	308	14	14	656	18	651	0.0	540
MS.gene57710.t1	AT1G55130	42.511	661	321	12	16	656	16	637	0.0	538
MS.gene57710.t1	AT2G24170	42.140	458	211	9	22	462	22	442	5.20e-120	365
MS.gene57710.t1	AT2G01970	27.889	649	383	16	20	656	17	592	3.06e-84	276
MS.gene57710.t1	AT1G08350	29.376	657	370	19	14	656	13	589	3.23e-81	268
MS.gene57710.t1	AT1G08350	29.376	657	370	19	14	656	13	589	3.23e-81	268
MS.gene57710.t1	AT1G14670	28.947	646	380	16	20	656	17	592	3.11e-80	266
MS.gene57710.t1	AT5G37310	27.717	635	378	15	32	656	30	593	3.59e-78	260
MS.gene57710.t1	AT5G37310	27.717	635	378	15	32	656	30	593	3.59e-78	260
MS.gene57710.t1	AT1G08350	28.351	582	329	15	88	656	2	508	1.38e-67	230
MS.gene57710.t1	AT1G08350	28.881	599	324	19	14	598	13	523	3.67e-67	229
MS.gene57710.t1	AT1G10950	36.691	417	246	9	250	656	181	589	9.14e-65	224

Find 183 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTGGTCTCCTCATATTTA+TGG	0.158373	8.3:+9314536	None:intergenic
ATCAAATACAAGATAGTTTA+TGG	0.166753	8.3:+9313265	None:intergenic
CAATCTTGAACTTCCTATTA+TGG	0.180368	8.3:-9313730	MS.gene57710:CDS
TTGGGTTCCTCTCTTCATTC+TGG	0.207220	8.3:-9313151	MS.gene57710:CDS
GAGGAATTTCTCTAGGAATT+TGG	0.209657	8.3:+9313564	None:intergenic
CCCTGTTGGTATTAAGATTC+AGG	0.247344	8.3:-9314602	MS.gene57710:CDS
TCCTTCTTTGTTGTGTATAA+TGG	0.248630	8.3:-9314005	MS.gene57710:CDS
TTCTCAGATCCTTCCTTAAC+AGG	0.259590	8.3:+9314469	None:intergenic
GGATCGAGAATTCGCCTTAT+AGG	0.263740	8.3:-9314807	MS.gene57710:CDS
ATGGTGATCACTTTCCTTGC+TGG	0.283624	8.3:-9314172	MS.gene57710:CDS
GGTGATGGAGTTCAGATTCT+TGG	0.295289	8.3:-9313980	MS.gene57710:CDS
ATTTGTTTGGTGTTTCAATC+AGG	0.295668	8.3:-9315021	MS.gene57710:CDS
AGCGGCTGGCCTTCTTTAAT+AGG	0.301449	8.3:+9314313	None:intergenic
AATATAACAAAGAGAGAAAA+TGG	0.302358	8.3:+9313683	None:intergenic
TCTTTATCATGTCCAGCATT+TGG	0.304826	8.3:-9313466	MS.gene57710:CDS
GACCAATGTGGCTCGTGTTA+TGG	0.324827	8.3:-9314521	MS.gene57710:CDS
GGTAAACTATAATAACTAAA+TGG	0.337219	8.3:+9314898	None:intergenic
GGAATTTGGTTGGTTCTAAC+AGG	0.337867	8.3:+9313578	None:intergenic
TACTTTGGCTTCCCCATAAT+AGG	0.338322	8.3:+9313717	None:intergenic
AATCTTGAACTTCCTATTAT+GGG	0.344499	8.3:-9313729	MS.gene57710:CDS
TGTTTCTGCAACTCTGTACT+TGG	0.345457	8.3:-9313222	MS.gene57710:CDS
CTGGGTCATGGCTATTTGTT+TGG	0.351997	8.3:-9315034	MS.gene57710:CDS
TTGTTATATTGCTATTGCTT+TGG	0.352574	8.3:-9313670	MS.gene57710:CDS
TTATCTTCTTGAGAACTGTT+AGG	0.357759	8.3:-9314138	MS.gene57710:CDS
GTAAACATCTTAAACCTATA+AGG	0.359924	8.3:+9314793	None:intergenic
GGGTATGGTTTCTACCTTCC+GGG	0.364387	8.3:-9315000	MS.gene57710:CDS
ACCAATGTGGCTCGTGTTAT+GGG	0.372249	8.3:-9314520	MS.gene57710:CDS
CACAACAAAGAAGGATTTGA+TGG	0.373080	8.3:+9314013	None:intergenic
GGTGCTGGCACCCTTCCCTT+TGG	0.373371	8.3:-9313509	MS.gene57710:CDS
TCCTGAATCTTAATACCAAC+AGG	0.375336	8.3:+9314601	None:intergenic
CTTATCAAGCTCCTCATAAC+TGG	0.375567	8.3:+9314105	None:intergenic
CTCGATCCTCAATTCGTTGA+TGG	0.377219	8.3:-9314191	MS.gene57710:CDS
ACACTTGTTGGTGGCTACTT+TGG	0.378171	8.3:-9313626	MS.gene57710:CDS
GTCGATGGATCACATTTAAT+AGG	0.380805	8.3:+9314346	None:intergenic
CTTGGGATACTCACTCTTCA+TGG	0.381798	8.3:-9313204	MS.gene57710:CDS
TCTGTCCCACTTACACTTGT+TGG	0.386331	8.3:-9313638	MS.gene57710:CDS
TGGAAATCATTCTTTGCCTC+TGG	0.395692	8.3:-9313317	MS.gene57710:CDS
GTGATGGAGTTCAGATTCTT+GGG	0.403271	8.3:-9313979	MS.gene57710:CDS
TATCATGTCCAGCATTTGGA+TGG	0.407267	8.3:-9313462	MS.gene57710:CDS
TACCCACACAATTATGCAAT+TGG	0.410515	8.3:-9314973	MS.gene57710:CDS
GTACATGGTTGTTGGGTTTG+AGG	0.411511	8.3:-9314440	MS.gene57710:CDS
TAAAGAACAGCTCAATGAAA+AGG	0.422226	8.3:+9313483	None:intergenic
TGGTTGGAAGCTTGTGGTGG+GGG	0.425246	8.3:-9314050	MS.gene57710:CDS
AAGCCGGGGTACATGGTTGT+TGG	0.426097	8.3:-9314448	MS.gene57710:CDS
GTGCGGCGATCAGAAGGGTT+GGG	0.426321	8.3:-9313810	MS.gene57710:CDS
AGCCGGGGTACATGGTTGTT+GGG	0.426832	8.3:-9314447	MS.gene57710:CDS
ATATCAAGTGGCCATCTAGA+TGG	0.430148	8.3:-9314255	MS.gene57710:CDS
TCTGGTTGGAAGCTTGTGGT+GGG	0.432889	8.3:-9314052	MS.gene57710:CDS
AAGTACAGAGTTGCAGAAAC+AGG	0.445695	8.3:+9313224	None:intergenic
CAGATGAATGAGGAGTTATC+TGG	0.450439	8.3:-9314070	MS.gene57710:CDS
ATCTGGTTGGAAGCTTGTGG+TGG	0.453164	8.3:-9314053	MS.gene57710:CDS
ACAATATTGTTTGCTGCACT+TGG	0.457516	8.3:-9313944	MS.gene57710:CDS
ATGGAATGGCTCCTGTACTT+TGG	0.461027	8.3:+9313702	None:intergenic
TTGGTGTTTCAATCAGGGTA+TGG	0.463389	8.3:-9315015	MS.gene57710:CDS
TGCACCTCTGTGTGGAGGAT+TGG	0.467543	8.3:-9313349	MS.gene57710:CDS
TCTTCTGTGAAGTTGGATTG+AGG	0.467795	8.3:-9313110	None:intergenic
ATCTGAGAAGCCGGGGTACA+TGG	0.468660	8.3:-9314455	MS.gene57710:CDS
AGGGTATGGTTTCTACCTTC+CGG	0.473489	8.3:-9315001	MS.gene57710:CDS
GAGGGATCTGGCCAGTTATG+AGG	0.477456	8.3:-9314116	MS.gene57710:CDS
GAGGATGAAGAAGGCAATAC+CGG	0.480232	8.3:+9313754	None:intergenic
TGTTCTTGTCCATAAATATG+AGG	0.481033	8.3:-9314545	MS.gene57710:CDS
AGGGTTGGGTTTCAGTTGCA+TGG	0.484208	8.3:-9313796	MS.gene57710:CDS
AAGTAAGCATCCCATCTAGA+TGG	0.484918	8.3:+9314244	None:intergenic
CGTGCGGCGATCAGAAGGGT+TGG	0.485992	8.3:-9313811	MS.gene57710:CDS
AAGGATAGTGCTGAGAATCT+AGG	0.486339	8.3:-9314850	MS.gene57710:CDS
GTCCCACTTACACTTGTTGG+TGG	0.488890	8.3:-9313635	MS.gene57710:CDS
TTTGTTTGGTGTTTCAATCA+GGG	0.489983	8.3:-9315020	MS.gene57710:CDS
CAAATTCAAATTCTGGGTCA+TGG	0.490284	8.3:-9315046	MS.gene57710:CDS
TTGTGTATAATGGTTGGTGA+TGG	0.492346	8.3:-9313995	MS.gene57710:CDS
ACTCGCGTGCGGCGATCAGA+AGG	0.493442	8.3:-9313816	MS.gene57710:CDS
TACCTACGAGGTAACGTTTG+AGG	0.495432	8.3:-9314284	MS.gene57710:CDS
ATGGGATGCTTACTTGAAGA+TGG	0.497074	8.3:-9314236	MS.gene57710:CDS
CAGTTATGAGGAGCTTGATA+AGG	0.497655	8.3:-9314104	MS.gene57710:CDS
ATGATACTTGGTATTGCTGC+TGG	0.498593	8.3:-9313866	MS.gene57710:CDS
CTCAATGAAAAGGGTTCCAA+AGG	0.503492	8.3:+9313493	None:intergenic
GATCGAGAACCAATGGACTT+TGG	0.504056	8.3:+9314207	None:intergenic
CGTAGGTAAACGCAAGCGGC+TGG	0.506527	8.3:+9314299	None:intergenic
TGTTGGTGACAGGCACAGTT+GGG	0.507832	8.3:-9313169	MS.gene57710:CDS
AAAGCTGCTTGTTTCTTCCC+CGG	0.509099	8.3:-9313773	MS.gene57710:CDS
TGCAATTGGTGATGAATTGT+CGG	0.510847	8.3:-9314959	MS.gene57710:CDS
TGGAGGATTGGAGATGGTGG+TGG	0.512530	8.3:-9313337	MS.gene57710:CDS
CAAGAACATTAAATCTAAGA+TGG	0.515293	8.3:+9314560	None:intergenic
CTAGCAATCATGTTGGTGAC+AGG	0.515495	8.3:-9313179	MS.gene57710:CDS
GAGAACTGTTAGGAGGGATC+TGG	0.516130	8.3:-9314128	MS.gene57710:CDS
GAATCTAGGTGAGCTTCTCA+TGG	0.516684	8.3:-9314836	MS.gene57710:CDS
AAAGAACAGCTCAATGAAAA+GGG	0.520078	8.3:+9313484	None:intergenic
TCTTGTGGTGGTTTGTGCTG+AGG	0.521715	8.3:-9313393	MS.gene57710:CDS
TAGCCACCAACAAGTGTAAG+TGG	0.522399	8.3:+9313632	None:intergenic
GTGGCTCGTGTTATGGGAAC+CGG	0.523916	8.3:-9314514	MS.gene57710:CDS
ACCTGTGATAAGTGTTCCAC+GGG	0.526211	8.3:+9313904	None:intergenic
TGATCACCATCAACGAATTG+AGG	0.527193	8.3:+9314185	None:intergenic
AACACGGCCCATCCAAATGC+TGG	0.527959	8.3:+9313454	None:intergenic
TTCATGTCACCGGCCTCCCG+TGG	0.527959	8.3:-9313920	MS.gene57710:CDS
AAGGAAGGATCTGAGAAGCC+GGG	0.528711	8.3:-9314463	MS.gene57710:CDS
AATTTCTCTAGGAATTTGGT+TGG	0.529324	8.3:+9313568	None:intergenic
ATAAGTGTTCCACGGGAGGC+CGG	0.530499	8.3:+9313911	None:intergenic
ACCATTATACACAACAAAGA+AGG	0.534847	8.3:+9314004	None:intergenic
CTGGTTGGAAGCTTGTGGTG+GGG	0.535511	8.3:-9314051	MS.gene57710:CDS
AATCATCTCAGCCGCATCAC+CGG	0.536909	8.3:+9314495	None:intergenic
TCTTTGTTGTGTATAATGGT+TGG	0.537010	8.3:-9314001	MS.gene57710:CDS
TCACCAATTGCATAATTGTG+TGG	0.538717	8.3:+9314970	None:intergenic
CTTCTTGAGAACTGTTAGGA+GGG	0.539675	8.3:-9314134	MS.gene57710:CDS
ATAGGCATTGCCACAGTCGA+TGG	0.540885	8.3:+9314331	None:intergenic
CGCTGCTGAGGAATTTCTCT+AGG	0.541640	8.3:+9313557	None:intergenic
TAAGGAAGGATCTGAGAAGC+CGG	0.543364	8.3:-9314464	MS.gene57710:CDS
TCTTCTTGAGAACTGTTAGG+AGG	0.545064	8.3:-9314135	MS.gene57710:CDS
AGGATGAAGAAGGCAATACC+GGG	0.546046	8.3:+9313755	None:intergenic
ATCATGTCCAGCATTTGGAT+GGG	0.548416	8.3:-9313461	MS.gene57710:CDS
TATCAAGTGGCCATCTAGAT+GGG	0.549497	8.3:-9314254	MS.gene57710:CDS
GGATCACATTTAATAGGTGA+CGG	0.552812	8.3:+9314352	None:intergenic
TGGTGGCTACTTTGGAGCAA+AGG	0.554397	8.3:-9313618	MS.gene57710:CDS
ATGTTGGTGACAGGCACAGT+TGG	0.557996	8.3:-9313170	MS.gene57710:CDS
AATCTAGGTGAGCTTCTCAT+GGG	0.557996	8.3:-9314835	MS.gene57710:CDS
GGATGCTTACTTGAAGATGG+AGG	0.561604	8.3:-9314233	MS.gene57710:CDS
TTCTTCGTAAAACGAATAGC+AGG	0.562872	8.3:+9314655	None:intergenic
TGGAGGGAGCCAAAGTCCAT+TGG	0.566645	8.3:-9314216	MS.gene57710:CDS
TGAAAAGGGTTCCAAAGGGA+AGG	0.570056	8.3:+9313498	None:intergenic
CACCAATTGCATAATTGTGT+GGG	0.570143	8.3:+9314971	None:intergenic
TCTCAGATCCTTCCTTAACA+GGG	0.572609	8.3:+9314470	None:intergenic
TGGAGTTCAGATTCTTGGGA+TGG	0.573301	8.3:-9313975	MS.gene57710:CDS
GTGGCAATGCCTATTAAAGA+AGG	0.576450	8.3:-9314322	MS.gene57710:CDS
GATCACATTTAATAGGTGAC+GGG	0.577005	8.3:+9314353	None:intergenic
GTGTGGAGGATTGGAGATGG+TGG	0.579374	8.3:-9313340	MS.gene57710:CDS
CAAGATTGTGAGGATGAAGA+AGG	0.582999	8.3:+9313745	None:intergenic
GATGTTTACTAATGAGTCTG+AGG	0.583355	8.3:-9314779	MS.gene57710:CDS
GTTATCTGGTTGGAAGCTTG+TGG	0.588427	8.3:-9314056	MS.gene57710:CDS
TCCCATAACACGAGCCACAT+TGG	0.588765	8.3:+9314519	None:intergenic
TGCACTTGGATTCATGTCAC+CGG	0.589020	8.3:-9313930	MS.gene57710:CDS
GTTTCTGCAACTCTGTACTT+GGG	0.589552	8.3:-9313221	MS.gene57710:CDS
AGATGGACTGGGTACCCTGT+TGG	0.591209	8.3:-9314616	MS.gene57710:CDS
ATCTTGAACTTCCTATTATG+GGG	0.592990	8.3:-9313728	MS.gene57710:CDS
TTATGGGGAAGCCAAAGTAC+AGG	0.594384	8.3:-9313713	MS.gene57710:CDS
AATTGAGGATCGAGAACCAA+TGG	0.597715	8.3:+9314200	None:intergenic
GTTATGTTTCAGTTCGACTG+TGG	0.597723	8.3:-9313844	MS.gene57710:CDS
TCCCGTGGAACACTTATCAC+AGG	0.598031	8.3:-9313905	MS.gene57710:CDS
CTCGCGTGCGGCGATCAGAA+GGG	0.598186	8.3:-9313815	MS.gene57710:CDS
CATGGTTCTAGCAATCATGT+TGG	0.600699	8.3:-9313186	MS.gene57710:CDS
AAGAAGGATTTGATGGAGCA+CGG	0.601804	8.3:+9314020	None:intergenic
TACCTGTGATAAGTGTTCCA+CGG	0.602595	8.3:+9313903	None:intergenic
TCAATGAAAAGGGTTCCAAA+GGG	0.608377	8.3:+9313494	None:intergenic
TAATTGTGTGGGTAACTACC+CGG	0.608773	8.3:+9314982	None:intergenic
AACAAAGAGAGAAAATGGAA+TGG	0.610331	8.3:+9313688	None:intergenic
AGCTCAAGCACAGATGAATG+AGG	0.617907	8.3:-9314080	MS.gene57710:CDS
CTCAGATCCTTCCTTAACAG+GGG	0.621573	8.3:+9314471	None:intergenic
ATCTAGGTGAGCTTCTCATG+GGG	0.627364	8.3:-9314834	MS.gene57710:CDS
TCTGTGTGGAGGATTGGAGA+TGG	0.630692	8.3:-9313343	MS.gene57710:CDS
CACGACGGATACCGCTGCTG+AGG	0.631043	8.3:+9313545	None:intergenic
GATGCTTACTTGAAGATGGA+GGG	0.631078	8.3:-9314232	MS.gene57710:CDS
GAAAAGGGTTCCAAAGGGAA+GGG	0.631885	8.3:+9313499	None:intergenic
TGAATGAGGAGTTATCTGGT+TGG	0.637187	8.3:-9314066	MS.gene57710:CDS
TCAGATCCTTCCTTAACAGG+GGG	0.637373	8.3:+9314472	None:intergenic
TGTGATAAGTGTTCCACGGG+AGG	0.637733	8.3:+9313907	None:intergenic
TGGTTCTAACAGGATACTCA+AGG	0.638465	8.3:+9313588	None:intergenic
CTCAGCAGCGGTATCCGTCG+TGG	0.639819	8.3:-9313544	MS.gene57710:CDS
ACCCGAAAACATAGTAAACA+CGG	0.641523	8.3:+9313438	None:intergenic
TTGAGGAGAGTGATATCAAG+TGG	0.642653	8.3:-9314267	MS.gene57710:CDS
GCATTATGCATAATACTACA+AGG	0.643881	8.3:+9314412	None:intergenic
ATAGGAAGTTCAAGATTGTG+AGG	0.645085	8.3:+9313735	None:intergenic
AACCCAACAACCATGTACCC+CGG	0.645248	8.3:+9314445	None:intergenic
CTAGAGAAATTCCTCAGCAG+CGG	0.650903	8.3:-9313556	MS.gene57710:CDS
TTACATGCACCTCTGTGTGG+AGG	0.651300	8.3:-9313354	MS.gene57710:CDS
AACTTACATGCACCTCTGTG+TGG	0.652266	8.3:-9313357	MS.gene57710:CDS
CTCCTCAAACGTTACCTCGT+AGG	0.655284	8.3:+9314282	None:intergenic
AGGAAGGATCTGAGAAGCCG+GGG	0.657385	8.3:-9314462	MS.gene57710:CDS
AGCCACCAACAAGTGTAAGT+GGG	0.658396	8.3:+9313633	None:intergenic
ACCTCGTAGGTAAACGCAAG+CGG	0.659065	8.3:+9314295	None:intergenic
TGGTGATGAATTGTCGGTGA+AGG	0.659099	8.3:-9314953	MS.gene57710:CDS
GCCGCTTGCGTTTACCTACG+AGG	0.660211	8.3:-9314296	MS.gene57710:CDS
ACACGAACCAGAATGAAGAG+AGG	0.661550	8.3:+9313144	None:intergenic
TGTTATGGGAACCGGTGATG+CGG	0.663081	8.3:-9314506	MS.gene57710:CDS
TAACAAGAACAATACCAGCA+AGG	0.672966	8.3:+9314158	None:intergenic
CTAACAGGATACTCAAGGTG+AGG	0.675817	8.3:+9313593	None:intergenic
TTTGATCTCAAGAATCTGAG+TGG	0.676176	8.3:-9313248	MS.gene57710:CDS
CTGTGGAGAACACTCGCGTG+CGG	0.676822	8.3:-9313827	MS.gene57710:CDS
CCTGAATCTTAATACCAACA+GGG	0.677762	8.3:+9314602	None:intergenic
GTACAGTGCAACAGAACCAG+AGG	0.685889	8.3:+9313301	None:intergenic
TAAATGTGATCCATCGACTG+TGG	0.688100	8.3:-9314341	MS.gene57710:CDS
CCTAGAACTAAAAGCCACGA+CGG	0.690997	8.3:+9313530	None:intergenic
TGTGTGGGTAACTACCCGGA+AGG	0.693942	8.3:+9314986	None:intergenic
TCTAGGTGAGCTTCTCATGG+GGG	0.697948	8.3:-9314833	MS.gene57710:CDS
TAAATATGAGGAGACCAATG+TGG	0.708708	8.3:-9314533	MS.gene57710:CDS
ATCTCCAATCCTCCACACAG+AGG	0.744593	8.3:+9313345	None:intergenic
GGATGAAGAAGGCAATACCG+GGG	0.767636	8.3:+9313756	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATAACAAAGAGAGAAAA+TGG	+	chr8.3:9314492-9314511	None:intergenic	20.0%
!!	ATCAAATACAAGATAGTTTA+TGG	+	chr8.3:9314910-9314929	None:intergenic	20.0%
!!	GGTAAACTATAATAACTAAA+TGG	+	chr8.3:9313277-9313296	None:intergenic	20.0%
!!!	TTTTCACAAATTCAAATTCT+GGG	-	chr8.3:9313120-9313139	MS.gene57710:CDS	20.0%
!	AATCTTGAACTTCCTATTAT+GGG	-	chr8.3:9314443-9314462	MS.gene57710:CDS	25.0%
!	CAAGAACATTAAATCTAAGA+TGG	+	chr8.3:9313615-9313634	None:intergenic	25.0%
!	GTAAACATCTTAAACCTATA+AGG	+	chr8.3:9313382-9313401	None:intergenic	25.0%
!!	CTTTTCACAAATTCAAATTC+TGG	-	chr8.3:9313119-9313138	MS.gene57710:CDS	25.0%
!!	TTGTTATATTGCTATTGCTT+TGG	-	chr8.3:9314502-9314521	MS.gene57710:CDS	25.0%
	AAAGAACAGCTCAATGAAAA+GGG	+	chr8.3:9314691-9314710	None:intergenic	30.0%
	AACAAAGAGAGAAAATGGAA+TGG	+	chr8.3:9314487-9314506	None:intergenic	30.0%
	ACCATTATACACAACAAAGA+AGG	+	chr8.3:9314171-9314190	None:intergenic	30.0%
	AGTTCAAGATCTTGAAAAAG+AGG	-	chr8.3:9313452-9313471	MS.gene57710:CDS	30.0%
	ATCTTGAACTTCCTATTATG+GGG	-	chr8.3:9314444-9314463	MS.gene57710:CDS	30.0%
	CAATCTTGAACTTCCTATTA+TGG	-	chr8.3:9314442-9314461	MS.gene57710:CDS	30.0%
	GCATTATGCATAATACTACA+AGG	+	chr8.3:9313763-9313782	None:intergenic	30.0%
	TAAAGAACAGCTCAATGAAA+AGG	+	chr8.3:9314692-9314711	None:intergenic	30.0%
	TGTTCTTGTCCATAAATATG+AGG	-	chr8.3:9313627-9313646	MS.gene57710:CDS	30.0%
	TTATCTTCTTGAGAACTGTT+AGG	-	chr8.3:9314034-9314053	MS.gene57710:CDS	30.0%
!	AATTTCTCTAGGAATTTGGT+TGG	+	chr8.3:9314607-9314626	None:intergenic	30.0%
!	TACCTTTTTGTAAACCTCAA+GGG	-	chr8.3:9313293-9313312	MS.gene57710:CDS	30.0%
!	TCCTTCTTTGTTGTGTATAA+TGG	-	chr8.3:9314167-9314186	MS.gene57710:CDS	30.0%
!	TCTTTGTTGTGTATAATGGT+TGG	-	chr8.3:9314171-9314190	MS.gene57710:CDS	30.0%
!	TTACCTTTTTGTAAACCTCA+AGG	-	chr8.3:9313292-9313311	MS.gene57710:CDS	30.0%
!!	AAGATGAGTACTTTTTGAGA+TGG	-	chr8.3:9313539-9313558	MS.gene57710:CDS	30.0%
!!	ATTTGTTTGGTGTTTCAATC+AGG	-	chr8.3:9313151-9313170	MS.gene57710:CDS	30.0%
!!	CTGTTTTTCTACATGATACT+TGG	-	chr8.3:9314294-9314313	MS.gene57710:CDS	30.0%
!!	TTTGTTTGGTGTTTCAATCA+GGG	-	chr8.3:9313152-9313171	MS.gene57710:CDS	30.0%
!!!	CATTGTTTTGATTCTTCTTG+TGG	-	chr8.3:9314764-9314783	MS.gene57710:CDS	30.0%
	ACCCGAAAACATAGTAAACA+CGG	+	chr8.3:9314737-9314756	None:intergenic	35.0%
	ATAGGAAGTTCAAGATTGTG+AGG	+	chr8.3:9314440-9314459	None:intergenic	35.0%
	CAAATTCAAATTCTGGGTCA+TGG	-	chr8.3:9313126-9313145	MS.gene57710:CDS	35.0%
	CACAACAAAGAAGGATTTGA+TGG	+	chr8.3:9314162-9314181	None:intergenic	35.0%
	CACCAATTGCATAATTGTGT+GGG	+	chr8.3:9313204-9313223	None:intergenic	35.0%
	CCTGAATCTTAATACCAACA+GGG	+	chr8.3:9313573-9313592	None:intergenic	35.0%
	GATCACATTTAATAGGTGAC+GGG	+	chr8.3:9313822-9313841	None:intergenic	35.0%
	GATGTTTACTAATGAGTCTG+AGG	-	chr8.3:9313393-9313412	MS.gene57710:CDS	35.0%
	GGATCACATTTAATAGGTGA+CGG	+	chr8.3:9313823-9313842	None:intergenic	35.0%
	GTCGATGGATCACATTTAAT+AGG	+	chr8.3:9313829-9313848	None:intergenic	35.0%
	TAAATATGAGGAGACCAATG+TGG	-	chr8.3:9313639-9313658	MS.gene57710:CDS	35.0%
	TAACAAGAACAATACCAGCA+AGG	+	chr8.3:9314017-9314036	None:intergenic	35.0%
	TACCCACACAATTATGCAAT+TGG	-	chr8.3:9313199-9313218	MS.gene57710:CDS	35.0%
	TCAATGAAAAGGGTTCCAAA+GGG	+	chr8.3:9314681-9314700	None:intergenic	35.0%
	TCACCAATTGCATAATTGTG+TGG	+	chr8.3:9313205-9313224	None:intergenic	35.0%
	TCCTGAATCTTAATACCAAC+AGG	+	chr8.3:9313574-9313593	None:intergenic	35.0%
	TCTTTATCATGTCCAGCATT+TGG	-	chr8.3:9314706-9314725	MS.gene57710:CDS	35.0%
	TTCTTCGTAAAACGAATAGC+AGG	+	chr8.3:9313520-9313539	None:intergenic	35.0%
	TTTGATCTCAAGAATCTGAG+TGG	-	chr8.3:9314924-9314943	MS.gene57710:CDS	35.0%
!	ACAATATTGTTTGCTGCACT+TGG	-	chr8.3:9314228-9314247	MS.gene57710:CDS	35.0%
!	ACCTTTTTGTAAACCTCAAG+GGG	-	chr8.3:9313294-9313313	MS.gene57710:CDS	35.0%
!	CATTGGTCTCCTCATATTTA+TGG	+	chr8.3:9313639-9313658	None:intergenic	35.0%
!	GAGGAATTTCTCTAGGAATT+TGG	+	chr8.3:9314611-9314630	None:intergenic	35.0%
!!	CTTGTTTTCTTCTGTGAAGT+TGG	-	chr8.3:9315055-9315074	MS.gene57710:CDS	35.0%
!!	TGCAATTGGTGATGAATTGT+CGG	-	chr8.3:9313213-9313232	MS.gene57710:CDS	35.0%
!!	TTGTGTATAATGGTTGGTGA+TGG	-	chr8.3:9314177-9314196	MS.gene57710:CDS	35.0%
!!!	AGTACTTTTTGAGATGGACT+GGG	-	chr8.3:9313545-9313564	MS.gene57710:CDS	35.0%
!!!	TGTTTTGATTCTTCTTGTGG+TGG	-	chr8.3:9314767-9314786	MS.gene57710:CDS	35.0%
	AAGAAGGATTTGATGGAGCA+CGG	+	chr8.3:9314155-9314174	None:intergenic	40.0%
	AAGTAAGCATCCCATCTAGA+TGG	+	chr8.3:9313931-9313950	None:intergenic	40.0%
	AAGTACAGAGTTGCAGAAAC+AGG	+	chr8.3:9314951-9314970	None:intergenic	40.0%
	AATCTAGGTGAGCTTCTCAT+GGG	-	chr8.3:9313337-9313356	MS.gene57710:CDS	40.0%
	AATTGAGGATCGAGAACCAA+TGG	+	chr8.3:9313975-9313994	None:intergenic	40.0%
	ATATCAAGTGGCCATCTAGA+TGG	-	chr8.3:9313917-9313936	MS.gene57710:CDS	40.0%
	ATCATGTCCAGCATTTGGAT+GGG	-	chr8.3:9314711-9314730	MS.gene57710:CDS	40.0%
	ATGATACTTGGTATTGCTGC+TGG	-	chr8.3:9314306-9314325	MS.gene57710:CDS	40.0%
	ATGGGATGCTTACTTGAAGA+TGG	-	chr8.3:9313936-9313955	MS.gene57710:CDS	40.0%
	CAAGATTGTGAGGATGAAGA+AGG	+	chr8.3:9314430-9314449	None:intergenic	40.0%
	CAGATGAATGAGGAGTTATC+TGG	-	chr8.3:9314102-9314121	MS.gene57710:CDS	40.0%
	CAGTTATGAGGAGCTTGATA+AGG	-	chr8.3:9314068-9314087	MS.gene57710:CDS	40.0%
	CCCTGTTGGTATTAAGATTC+AGG	-	chr8.3:9313570-9313589	MS.gene57710:CDS	40.0%
	CTCAATGAAAAGGGTTCCAA+AGG	+	chr8.3:9314682-9314701	None:intergenic	40.0%
	CTTATCAAGCTCCTCATAAC+TGG	+	chr8.3:9314070-9314089	None:intergenic	40.0%
	CTTCTTGAGAACTGTTAGGA+GGG	-	chr8.3:9314038-9314057	MS.gene57710:CDS	40.0%
	GATGCTTACTTGAAGATGGA+GGG	-	chr8.3:9313940-9313959	MS.gene57710:CDS	40.0%
	GTGGCAATGCCTATTAAAGA+AGG	-	chr8.3:9313850-9313869	MS.gene57710:CDS	40.0%
	GTTATGTTTCAGTTCGACTG+TGG	-	chr8.3:9314328-9314347	MS.gene57710:CDS	40.0%
	GTTTCTGCAACTCTGTACTT+GGG	-	chr8.3:9314951-9314970	MS.gene57710:CDS	40.0%
	TAATTGTGTGGGTAACTACC+CGG	+	chr8.3:9313193-9313212	None:intergenic	40.0%
	TACCTGTGATAAGTGTTCCA+CGG	+	chr8.3:9314272-9314291	None:intergenic	40.0%
	TATCAAGTGGCCATCTAGAT+GGG	-	chr8.3:9313918-9313937	MS.gene57710:CDS	40.0%
	TATCATGTCCAGCATTTGGA+TGG	-	chr8.3:9314710-9314729	MS.gene57710:CDS	40.0%
	TCTCAGATCCTTCCTTAACA+GGG	+	chr8.3:9313705-9313724	None:intergenic	40.0%
	TCTTCTTGAGAACTGTTAGG+AGG	-	chr8.3:9314037-9314056	MS.gene57710:CDS	40.0%
	TGAATGAGGAGTTATCTGGT+TGG	-	chr8.3:9314106-9314125	MS.gene57710:CDS	40.0%
	TGATCACCATCAACGAATTG+AGG	+	chr8.3:9313990-9314009	None:intergenic	40.0%
	TGGAAATCATTCTTTGCCTC+TGG	-	chr8.3:9314855-9314874	MS.gene57710:CDS	40.0%
	TTCTCAGATCCTTCCTTAAC+AGG	+	chr8.3:9313706-9313725	None:intergenic	40.0%
	TTGAGGAGAGTGATATCAAG+TGG	-	chr8.3:9313905-9313924	MS.gene57710:CDS	40.0%
!	AAGGATAGTGCTGAGAATCT+AGG	-	chr8.3:9313322-9313341	MS.gene57710:CDS	40.0%
!	CCTTTTTGTAAACCTCAAGG+GGG	-	chr8.3:9313295-9313314	MS.gene57710:CDS	40.0%
!	GCCGTGTTTACTATGTTTTC+GGG	-	chr8.3:9314733-9314752	MS.gene57710:CDS	40.0%
!	GGCCGTGTTTACTATGTTTT+CGG	-	chr8.3:9314732-9314751	MS.gene57710:CDS	40.0%
!	GTGATGGAGTTCAGATTCTT+GGG	-	chr8.3:9314193-9314212	MS.gene57710:CDS	40.0%
!	TAAATGTGATCCATCGACTG+TGG	-	chr8.3:9313831-9313850	MS.gene57710:CDS	40.0%
!	TACTTTGGCTTCCCCATAAT+AGG	+	chr8.3:9314458-9314477	None:intergenic	40.0%
!	TGTTTCTGCAACTCTGTACT+TGG	-	chr8.3:9314950-9314969	MS.gene57710:CDS	40.0%
!	TTGGTGTTTCAATCAGGGTA+TGG	-	chr8.3:9313157-9313176	MS.gene57710:CDS	40.0%
!!	CATGGTTCTAGCAATCATGT+TGG	-	chr8.3:9314986-9315005	MS.gene57710:CDS	40.0%
!!	GGAATTTGGTTGGTTCTAAC+AGG	+	chr8.3:9314597-9314616	None:intergenic	40.0%
!!	TGGTTCTAACAGGATACTCA+AGG	+	chr8.3:9314587-9314606	None:intergenic	40.0%
!!!	GAGTACTTTTTGAGATGGAC+TGG	-	chr8.3:9313544-9313563	MS.gene57710:CDS	40.0%
	AACTTACATGCACCTCTGTG+TGG	-	chr8.3:9314815-9314834	MS.gene57710:CDS	45.0%
	ACACGAACCAGAATGAAGAG+AGG	+	chr8.3:9315031-9315050	None:intergenic	45.0%
	ACCAATGTGGCTCGTGTTAT+GGG	-	chr8.3:9313652-9313671	MS.gene57710:CDS	45.0%
	ACCTGTGATAAGTGTTCCAC+GGG	+	chr8.3:9314271-9314290	None:intergenic	45.0%
	AGCCACCAACAAGTGTAAGT+GGG	+	chr8.3:9314542-9314561	None:intergenic	45.0%
	AGCTCAAGCACAGATGAATG+AGG	-	chr8.3:9314092-9314111	MS.gene57710:CDS	45.0%
	AGGATGAAGAAGGCAATACC+GGG	+	chr8.3:9314420-9314439	None:intergenic	45.0%
	AGGGTATGGTTTCTACCTTC+CGG	-	chr8.3:9313171-9313190	MS.gene57710:CDS	45.0%
	ATCTAGGTGAGCTTCTCATG+GGG	-	chr8.3:9313338-9313357	MS.gene57710:CDS	45.0%
	ATGATTCCCCCTGTTAAGGA+AGG	-	chr8.3:9313694-9313713	MS.gene57710:CDS	45.0%
	ATGGAATGGCTCCTGTACTT+TGG	+	chr8.3:9314473-9314492	None:intergenic	45.0%
	ATGGTGATCACTTTCCTTGC+TGG	-	chr8.3:9314000-9314019	MS.gene57710:CDS	45.0%
	CCCCCTTGAGGTTTACAAAA+AGG	+	chr8.3:9313298-9313317	None:intergenic	45.0%
	CCTAGAACTAAAAGCCACGA+CGG	+	chr8.3:9314645-9314664	None:intergenic	45.0%
	CTAACAGGATACTCAAGGTG+AGG	+	chr8.3:9314582-9314601	None:intergenic	45.0%
	CTAGAGAAATTCCTCAGCAG+CGG	-	chr8.3:9314616-9314635	MS.gene57710:CDS	45.0%
	CTCAGATCCTTCCTTAACAG+GGG	+	chr8.3:9313704-9313723	None:intergenic	45.0%
	CTCGATCCTCAATTCGTTGA+TGG	-	chr8.3:9313981-9314000	MS.gene57710:CDS	45.0%
	CTGGGTCATGGCTATTTGTT+TGG	-	chr8.3:9313138-9313157	MS.gene57710:CDS	45.0%
	CTTGGGATACTCACTCTTCA+TGG	-	chr8.3:9314968-9314987	MS.gene57710:CDS	45.0%
	GAAAAGGGTTCCAAAGGGAA+GGG	+	chr8.3:9314676-9314695	None:intergenic	45.0%
	GAATCTAGGTGAGCTTCTCA+TGG	-	chr8.3:9313336-9313355	MS.gene57710:CDS	45.0%
	GAGGATGAAGAAGGCAATAC+CGG	+	chr8.3:9314421-9314440	None:intergenic	45.0%
	GGATCGAGAATTCGCCTTAT+AGG	-	chr8.3:9313365-9313384	MS.gene57710:CDS	45.0%
	GGATGCTTACTTGAAGATGG+AGG	-	chr8.3:9313939-9313958	MS.gene57710:CDS	45.0%
	GTACATGGTTGTTGGGTTTG+AGG	-	chr8.3:9313732-9313751	MS.gene57710:CDS	45.0%
	GTTATCTGGTTGGAAGCTTG+TGG	-	chr8.3:9314116-9314135	MS.gene57710:CDS	45.0%
	TAAGGAAGGATCTGAGAAGC+CGG	-	chr8.3:9313708-9313727	MS.gene57710:CDS	45.0%
	TACCTACGAGGTAACGTTTG+AGG	-	chr8.3:9313888-9313907	MS.gene57710:CDS	45.0%
	TAGCCACCAACAAGTGTAAG+TGG	+	chr8.3:9314543-9314562	None:intergenic	45.0%
	TCAGATCCTTCCTTAACAGG+GGG	+	chr8.3:9313703-9313722	None:intergenic	45.0%
	TCTGTCCCACTTACACTTGT+TGG	-	chr8.3:9314534-9314553	MS.gene57710:CDS	45.0%
	TGAAAAGGGTTCCAAAGGGA+AGG	+	chr8.3:9314677-9314696	None:intergenic	45.0%
	TGAGATGATTCCCCCTGTTA+AGG	-	chr8.3:9313690-9313709	MS.gene57710:CDS	45.0%
	TGCACTTGGATTCATGTCAC+CGG	-	chr8.3:9314242-9314261	MS.gene57710:CDS	45.0%
	TGGAGTTCAGATTCTTGGGA+TGG	-	chr8.3:9314197-9314216	MS.gene57710:CDS	45.0%
	TTATGGGGAAGCCAAAGTAC+AGG	-	chr8.3:9314459-9314478	MS.gene57710:CDS	45.0%
	TTGGGTTCCTCTCTTCATTC+TGG	-	chr8.3:9315021-9315040	MS.gene57710:CDS	45.0%
!	AAAGCTGCTTGTTTCTTCCC+CGG	-	chr8.3:9314399-9314418	MS.gene57710:CDS	45.0%
!	GATCGAGAACCAATGGACTT+TGG	+	chr8.3:9313968-9313987	None:intergenic	45.0%
!	TGGTGATGAATTGTCGGTGA+AGG	-	chr8.3:9313219-9313238	MS.gene57710:CDS	45.0%
!!	ACACTTGTTGGTGGCTACTT+TGG	-	chr8.3:9314546-9314565	MS.gene57710:CDS	45.0%
!!	CTAGCAATCATGTTGGTGAC+AGG	-	chr8.3:9314993-9315012	MS.gene57710:CDS	45.0%
!!	GGTGATGGAGTTCAGATTCT+TGG	-	chr8.3:9314192-9314211	MS.gene57710:CDS	45.0%
!!	TAAACCTCAAGGGGGTGTTA+AGG	-	chr8.3:9313303-9313322	MS.gene57710:CDS	45.0%
!!!	TGGCTTTTAGTTCTAGGTGC+TGG	-	chr8.3:9314648-9314667	MS.gene57710:CDS	45.0%
	AACCCAACAACCATGTACCC+CGG	+	chr8.3:9313730-9313749	None:intergenic	50.0%
	AAGGAAGGATCTGAGAAGCC+GGG	-	chr8.3:9313709-9313728	MS.gene57710:CDS	50.0%
	AATCATCTCAGCCGCATCAC+CGG	+	chr8.3:9313680-9313699	None:intergenic	50.0%
	ACCTCGTAGGTAAACGCAAG+CGG	+	chr8.3:9313880-9313899	None:intergenic	50.0%
	AGGGTTGGGTTTCAGTTGCA+TGG	-	chr8.3:9314376-9314395	MS.gene57710:CDS	50.0%
	ATCTCCAATCCTCCACACAG+AGG	+	chr8.3:9314830-9314849	None:intergenic	50.0%
	ATCTGGTTGGAAGCTTGTGG+TGG	-	chr8.3:9314119-9314138	MS.gene57710:CDS	50.0%
	CGCTGCTGAGGAATTTCTCT+AGG	+	chr8.3:9314618-9314637	None:intergenic	50.0%
	CTATCCTTAACACCCCCTTG+AGG	+	chr8.3:9313310-9313329	None:intergenic	50.0%
	CTCCTCAAACGTTACCTCGT+AGG	+	chr8.3:9313893-9313912	None:intergenic	50.0%
	GACCAATGTGGCTCGTGTTA+TGG	-	chr8.3:9313651-9313670	MS.gene57710:CDS	50.0%
	GAGAACTGTTAGGAGGGATC+TGG	-	chr8.3:9314044-9314063	MS.gene57710:CDS	50.0%
	GGATGAAGAAGGCAATACCG+GGG	+	chr8.3:9314419-9314438	None:intergenic	50.0%
	GGGTATGGTTTCTACCTTCC+GGG	-	chr8.3:9313172-9313191	MS.gene57710:CDS	50.0%
	GTACAGTGCAACAGAACCAG+AGG	+	chr8.3:9314874-9314893	None:intergenic	50.0%
	TCCCATAACACGAGCCACAT+TGG	+	chr8.3:9313656-9313675	None:intergenic	50.0%
	TCCCGTGGAACACTTATCAC+AGG	-	chr8.3:9314267-9314286	MS.gene57710:CDS	50.0%
	TCTAGGTGAGCTTCTCATGG+GGG	-	chr8.3:9313339-9313358	MS.gene57710:CDS	50.0%
	TCTGGTTGGAAGCTTGTGGT+GGG	-	chr8.3:9314120-9314139	MS.gene57710:CDS	50.0%
	TCTGTGTGGAGGATTGGAGA+TGG	-	chr8.3:9314829-9314848	MS.gene57710:CDS	50.0%
	TGTGATAAGTGTTCCACGGG+AGG	+	chr8.3:9314268-9314287	None:intergenic	50.0%
	TGTTATGGGAACCGGTGATG+CGG	-	chr8.3:9313666-9313685	MS.gene57710:CDS	50.0%
	TTACATGCACCTCTGTGTGG+AGG	-	chr8.3:9314818-9314837	MS.gene57710:CDS	50.0%
!	ATAGGCATTGCCACAGTCGA+TGG	+	chr8.3:9313844-9313863	None:intergenic	50.0%
!	GTCCCACTTACACTTGTTGG+TGG	-	chr8.3:9314537-9314556	MS.gene57710:CDS	50.0%
!	TCTTGTGGTGGTTTGTGCTG+AGG	-	chr8.3:9314779-9314798	MS.gene57710:CDS	50.0%
!!	AGCGGCTGGCCTTCTTTAAT+AGG	+	chr8.3:9313862-9313881	None:intergenic	50.0%
!!	ATGTTGGTGACAGGCACAGT+TGG	-	chr8.3:9315002-9315021	MS.gene57710:CDS	50.0%
!!	TGGTGGCTACTTTGGAGCAA+AGG	-	chr8.3:9314554-9314573	MS.gene57710:CDS	50.0%
!!	TGTTGGTGACAGGCACAGTT+GGG	-	chr8.3:9315003-9315022	MS.gene57710:CDS	50.0%
!!!	CCGTCGTGGCTTTTAGTTCT+AGG	-	chr8.3:9314642-9314661	MS.gene57710:CDS	50.0%
	AACACGGCCCATCCAAATGC+TGG	+	chr8.3:9314721-9314740	None:intergenic	55.0%
	AAGCCGGGGTACATGGTTGT+TGG	-	chr8.3:9313724-9313743	MS.gene57710:CDS	55.0%
	AGCCGGGGTACATGGTTGTT+GGG	-	chr8.3:9313725-9313744	MS.gene57710:CDS	55.0%
	AGGAAGGATCTGAGAAGCCG+GGG	-	chr8.3:9313710-9313729	MS.gene57710:CDS	55.0%
	ATAAGTGTTCCACGGGAGGC+CGG	+	chr8.3:9314264-9314283	None:intergenic	55.0%
	ATCTGAGAAGCCGGGGTACA+TGG	-	chr8.3:9313717-9313736	MS.gene57710:CDS	55.0%
	CTGGTTGGAAGCTTGTGGTG+GGG	-	chr8.3:9314121-9314140	MS.gene57710:CDS	55.0%
	GAGGGATCTGGCCAGTTATG+AGG	-	chr8.3:9314056-9314075	MS.gene57710:CDS	55.0%
	GTGAGCTTCTCATGGGGGAT+CGG	-	chr8.3:9313344-9313363	MS.gene57710:CDS	55.0%
	GTGGCTCGTGTTATGGGAAC+CGG	-	chr8.3:9313658-9313677	MS.gene57710:CDS	55.0%
	GTGTGGAGGATTGGAGATGG+TGG	-	chr8.3:9314832-9314851	MS.gene57710:CDS	55.0%
	TGCACCTCTGTGTGGAGGAT+TGG	-	chr8.3:9314823-9314842	MS.gene57710:CDS	55.0%
	TGGAGGGAGCCAAAGTCCAT+TGG	-	chr8.3:9313956-9313975	MS.gene57710:CDS	55.0%
	TGGTTGGAAGCTTGTGGTGG+GGG	-	chr8.3:9314122-9314141	MS.gene57710:CDS	55.0%
	TGTGTGGGTAACTACCCGGA+AGG	+	chr8.3:9313189-9313208	None:intergenic	55.0%
!	TGGAGGATTGGAGATGGTGG+TGG	-	chr8.3:9314835-9314854	MS.gene57710:CDS	55.0%
!!	AGATGGACTGGGTACCCTGT+TGG	-	chr8.3:9313556-9313575	MS.gene57710:CDS	55.0%
	CGTAGGTAAACGCAAGCGGC+TGG	+	chr8.3:9313876-9313895	None:intergenic	60.0%
	CTGTGGAGAACACTCGCGTG+CGG	-	chr8.3:9314345-9314364	MS.gene57710:CDS	60.0%
	GCCGCTTGCGTTTACCTACG+AGG	-	chr8.3:9313876-9313895	MS.gene57710:CDS	60.0%
	GTGCGGCGATCAGAAGGGTT+GGG	-	chr8.3:9314362-9314381	MS.gene57710:CDS	60.0%
	ACTCGCGTGCGGCGATCAGA+AGG	-	chr8.3:9314356-9314375	MS.gene57710:CDS	65.0%
	CACGACGGATACCGCTGCTG+AGG	+	chr8.3:9314630-9314649	None:intergenic	65.0%
	CGTGCGGCGATCAGAAGGGT+TGG	-	chr8.3:9314361-9314380	MS.gene57710:CDS	65.0%
	CTCAGCAGCGGTATCCGTCG+TGG	-	chr8.3:9314628-9314647	MS.gene57710:CDS	65.0%
	CTCGCGTGCGGCGATCAGAA+GGG	-	chr8.3:9314357-9314376	MS.gene57710:CDS	65.0%
	TTCATGTCACCGGCCTCCCG+TGG	-	chr8.3:9314252-9314271	MS.gene57710:CDS	65.0%
!!	GGTGCTGGCACCCTTCCCTT+TGG	-	chr8.3:9314663-9314682	MS.gene57710:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	9313112	9315082	9313112	ID=MS.gene57710
chr8.3	mRNA	9313112	9315082	9313112	ID=MS.gene57710.t1;Parent=MS.gene57710
chr8.3	exon	9313112	9315082	9313112	ID=MS.gene57710.t1.exon1;Parent=MS.gene57710.t1
chr8.3	CDS	9313112	9315082	9313112	ID=cds.MS.gene57710.t1;Parent=MS.gene57710.t1

Gene Sequence

>MS.gene57710

ATGGAGTCTTTTCACAAATTCAAATTCTGGGTCATGGCTATTTGTTTGGTGTTTCAATCAGGGTATGGTTTCTACCTTCCGGGTAGTTACCCACACAATTATGCAATTGGTGATGAATTGTCGGTGAAGGTGAATTCTATCACTTCAATTGATACTGAAATGCCATTTAGTTATTATAGTTTACCTTTTTGTAAACCTCAAGGGGGTGTTAAGGATAGTGCTGAGAATCTAGGTGAGCTTCTCATGGGGGATCGGATCGAGAATTCGCCTTATAGGTTTAAGATGTTTACTAATGAGTCTGAGGTGTTTTTGTGTCAAGTTGATAAATTGTCTGATGATCAGTTCAAGATCTTGAAAAAGAGGATTGATGAGATGTATCAAGTTAATCTTATACTTGATAATTTACCTGCTATTCGTTTTACGAAGAAAGATGAGTACTTTTTGAGATGGACTGGGTACCCTGTTGGTATTAAGATTCAGGATGTGTATTATTTGTTTAACCATCTTAGATTTAATGTTCTTGTCCATAAATATGAGGAGACCAATGTGGCTCGTGTTATGGGAACCGGTGATGCGGCTGAGATGATTCCCCCTGTTAAGGAAGGATCTGAGAAGCCGGGGTACATGGTTGTTGGGTTTGAGGTTATACCTTGTAGTATTATGCATAATGCTAATTCAGTTAAAAACTTGAAAATGTATGAAAAATACCCGTCACCTATTAAATGTGATCCATCGACTGTGGCAATGCCTATTAAAGAAGGCCAGCCGCTTGCGTTTACCTACGAGGTAACGTTTGAGGAGAGTGATATCAAGTGGCCATCTAGATGGGATGCTTACTTGAAGATGGAGGGAGCCAAAGTCCATTGGTTCTCGATCCTCAATTCGTTGATGGTGATCACTTTCCTTGCTGGTATTGTTCTTGTTATCTTCTTGAGAACTGTTAGGAGGGATCTGGCCAGTTATGAGGAGCTTGATAAGGAAGCTCAAGCACAGATGAATGAGGAGTTATCTGGTTGGAAGCTTGTGGTGGGGGATGTTTTCCGTGCTCCATCAAATCCTTCTTTGTTGTGTATAATGGTTGGTGATGGAGTTCAGATTCTTGGGATGGCTGTCGTGACAATATTGTTTGCTGCACTTGGATTCATGTCACCGGCCTCCCGTGGAACACTTATCACAGGTATGCTGTTTTTCTACATGATACTTGGTATTGCTGCTGGTTATGTTTCAGTTCGACTGTGGAGAACACTCGCGTGCGGCGATCAGAAGGGTTGGGTTTCAGTTGCATGGAAAGCTGCTTGTTTCTTCCCCGGTATTGCCTTCTTCATCCTCACAATCTTGAACTTCCTATTATGGGGAAGCCAAAGTACAGGAGCCATTCCATTTTCTCTCTTTGTTATATTGCTATTGCTTTGGTTCTGCATTTCTGTCCCACTTACACTTGTTGGTGGCTACTTTGGAGCAAAGGCACCTCACCTTGAGTATCCTGTTAGAACCAACCAAATTCCTAGAGAAATTCCTCAGCAGCGGTATCCGTCGTGGCTTTTAGTTCTAGGTGCTGGCACCCTTCCCTTTGGAACCCTTTTCATTGAGCTGTTCTTTATCATGTCCAGCATTTGGATGGGCCGTGTTTACTATGTTTTCGGGTTTCTCTTCATTGTTTTGATTCTTCTTGTGGTGGTTTGTGCTGAGGTATCTCTTGTTCTAACTTACATGCACCTCTGTGTGGAGGATTGGAGATGGTGGTGGAAATCATTCTTTGCCTCTGGTTCTGTTGCACTGTACATCTTTTTGTATTCCATAAACTATCTTGTATTTGATCTCAAGAATCTGAGTGGACCTGTTTCTGCAACTCTGTACTTGGGATACTCACTCTTCATGGTTCTAGCAATCATGTTGGTGACAGGCACAGTTGGGTTCCTCTCTTCATTCTGGTTCGTGTATTACTTGTTTTCTTCTGTGAAGTTGGATTGA

Protein sequence

>MS.gene57710.t1

MESFHKFKFWVMAICLVFQSGYGFYLPGSYPHNYAIGDELSVKVNSITSIDTEMPFSYYSLPFCKPQGGVKDSAENLGELLMGDRIENSPYRFKMFTNESEVFLCQVDKLSDDQFKILKKRIDEMYQVNLILDNLPAIRFTKKDEYFLRWTGYPVGIKIQDVYYLFNHLRFNVLVHKYEETNVARVMGTGDAAEMIPPVKEGSEKPGYMVVGFEVIPCSIMHNANSVKNLKMYEKYPSPIKCDPSTVAMPIKEGQPLAFTYEVTFEESDIKWPSRWDAYLKMEGAKVHWFSILNSLMVITFLAGIVLVIFLRTVRRDLASYEELDKEAQAQMNEELSGWKLVVGDVFRAPSNPSLLCIMVGDGVQILGMAVVTILFAALGFMSPASRGTLITGMLFFYMILGIAAGYVSVRLWRTLACGDQKGWVSVAWKAACFFPGIAFFILTILNFLLWGSQSTGAIPFSLFVILLLLWFCISVPLTLVGGYFGAKAPHLEYPVRTNQIPREIPQQRYPSWLLVLGAGTLPFGTLFIELFFIMSSIWMGRVYYVFGFLFIVLILLVVVCAEVSLVLTYMHLCVEDWRWWWKSFFASGSVALYIFLYSINYLVFDLKNLSGPVSATLYLGYSLFMVLAIMLVTGTVGFLSSFWFVYYLFSSVKLD