Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58740.t1 | XP_013458155.1 | 97.1 | 454 | 13 | 0 | 1 | 454 | 1 | 454 | 6.90E-255 | 889.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58740.t1 | Q9FSH9 | 33.0 | 469 | 257 | 17 | 7 | 450 | 9 | 445 | 5.3e-54 | 213.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58740.t1 | G8A053 | 97.1 | 454 | 13 | 0 | 1 | 454 | 1 | 454 | 5.0e-255 | 889.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene58740 | -0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene050133 | MS.gene58740 | 0.806536 | 7.84E-50 | -1.69E-46 |
| MS.gene050315 | MS.gene58740 | -0.803627 | 3.18E-49 | -1.69E-46 |
| MS.gene050329 | MS.gene58740 | 0.807336 | 5.31E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene58740 | 0.826834 | 2.18E-54 | -1.69E-46 |
| MS.gene051158 | MS.gene58740 | 0.818311 | 2.09E-52 | -1.69E-46 |
| MS.gene051996 | MS.gene58740 | 0.812472 | 4.17E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene58740 | 0.816395 | 5.65E-52 | -1.69E-46 |
| MS.gene052223 | MS.gene58740 | 0.870199 | 1.75E-66 | -1.69E-46 |
| MS.gene05233 | MS.gene58740 | 0.800371 | 1.49E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene58740 | 0.812856 | 3.43E-51 | -1.69E-46 |
| MS.gene055157 | MS.gene58740 | 0.820191 | 7.81E-53 | -1.69E-46 |
| MS.gene055601 | MS.gene58740 | 0.838318 | 3.09E-57 | -1.69E-46 |
| MS.gene056330 | MS.gene58740 | 0.839289 | 1.73E-57 | -1.69E-46 |
| MS.gene056690 | MS.gene58740 | 0.849559 | 2.99E-60 | -1.69E-46 |
| MS.gene056691 | MS.gene58740 | 0.828702 | 7.76E-55 | -1.69E-46 |
| MS.gene056899 | MS.gene58740 | 0.842759 | 2.12E-58 | -1.69E-46 |
| MS.gene058218 | MS.gene58740 | 0.805446 | 1.33E-49 | -1.69E-46 |
| MS.gene059892 | MS.gene58740 | 0.804676 | 1.93E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene58740 | 0.810304 | 1.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58740.t1 | MTR_4g117090 | 97.137 | 454 | 13 | 0 | 1 | 454 | 1 | 454 | 0.0 | 895 |
| MS.gene58740.t1 | MTR_2g022480 | 47.807 | 456 | 200 | 11 | 3 | 449 | 4 | 430 | 2.17e-118 | 353 |
| MS.gene58740.t1 | MTR_2g022370 | 49.231 | 455 | 188 | 13 | 1 | 450 | 1 | 417 | 7.47e-116 | 347 |
| MS.gene58740.t1 | MTR_2g022320 | 47.162 | 458 | 193 | 12 | 1 | 450 | 1 | 417 | 1.11e-114 | 343 |
| MS.gene58740.t1 | MTR_2g022430 | 48.341 | 422 | 183 | 11 | 37 | 450 | 27 | 421 | 1.45e-114 | 343 |
| MS.gene58740.t1 | MTR_2g022490 | 47.765 | 425 | 187 | 9 | 33 | 449 | 27 | 424 | 3.56e-114 | 342 |
| MS.gene58740.t1 | MTR_2g022380 | 48.584 | 459 | 189 | 14 | 1 | 450 | 1 | 421 | 8.28e-114 | 342 |
| MS.gene58740.t1 | MTR_2g022350 | 47.162 | 458 | 197 | 12 | 1 | 450 | 1 | 421 | 1.79e-111 | 335 |
| MS.gene58740.t1 | MTR_1g072420 | 45.626 | 423 | 204 | 10 | 33 | 451 | 29 | 429 | 2.10e-111 | 335 |
| MS.gene58740.t1 | MTR_2g022450 | 43.794 | 427 | 213 | 12 | 29 | 451 | 21 | 424 | 6.69e-109 | 329 |
| MS.gene58740.t1 | MTR_1g072450 | 43.839 | 422 | 212 | 9 | 33 | 451 | 29 | 428 | 3.30e-107 | 325 |
| MS.gene58740.t1 | MTR_1g093100 | 43.710 | 469 | 207 | 16 | 1 | 451 | 1 | 430 | 3.23e-101 | 309 |
| MS.gene58740.t1 | MTR_1g072380 | 40.909 | 440 | 232 | 9 | 21 | 452 | 27 | 446 | 5.74e-100 | 306 |
| MS.gene58740.t1 | MTR_2g022460 | 34.404 | 436 | 216 | 11 | 35 | 452 | 25 | 408 | 2.76e-70 | 229 |
| MS.gene58740.t1 | MTR_7g113790 | 33.548 | 465 | 266 | 15 | 1 | 454 | 1 | 433 | 1.64e-68 | 225 |
| MS.gene58740.t1 | MTR_0252s0030 | 49.545 | 220 | 97 | 5 | 202 | 419 | 140 | 347 | 9.66e-56 | 189 |
| MS.gene58740.t1 | MTR_1g093130 | 37.059 | 170 | 87 | 3 | 248 | 416 | 5 | 155 | 5.67e-23 | 95.9 |
| MS.gene58740.t1 | MTR_2g022330 | 64.912 | 57 | 16 | 1 | 396 | 452 | 34 | 86 | 1.31e-14 | 69.3 |
| MS.gene58740.t1 | MTR_3g065160 | 23.227 | 409 | 247 | 13 | 53 | 451 | 78 | 429 | 1.40e-13 | 72.8 |
| MS.gene58740.t1 | MTR_3g053710 | 24.697 | 413 | 223 | 13 | 47 | 437 | 115 | 461 | 9.41e-12 | 67.4 |
| MS.gene58740.t1 | MTR_7g117430 | 24.938 | 405 | 224 | 16 | 49 | 438 | 141 | 480 | 5.85e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58740.t1 | AT1G03220 | 43.142 | 452 | 219 | 12 | 5 | 451 | 9 | 427 | 1.83e-105 | 320 |
| MS.gene58740.t1 | AT1G03230 | 42.925 | 424 | 214 | 11 | 33 | 451 | 28 | 428 | 3.18e-101 | 309 |
| MS.gene58740.t1 | AT5G19110 | 34.597 | 422 | 223 | 14 | 37 | 451 | 26 | 401 | 2.10e-59 | 200 |
| MS.gene58740.t1 | AT5G19110 | 34.597 | 422 | 223 | 14 | 37 | 451 | 62 | 437 | 4.12e-59 | 200 |
| MS.gene58740.t1 | AT5G19100 | 36.301 | 438 | 201 | 14 | 25 | 452 | 18 | 387 | 2.29e-55 | 189 |
| MS.gene58740.t1 | AT5G19120 | 33.257 | 439 | 227 | 15 | 1 | 437 | 1 | 375 | 1.43e-50 | 176 |
| MS.gene58740.t1 | AT5G48430 | 38.267 | 277 | 145 | 7 | 177 | 452 | 152 | 403 | 4.14e-45 | 162 |
| MS.gene58740.t1 | AT3G25700 | 24.450 | 409 | 244 | 17 | 49 | 438 | 83 | 445 | 5.38e-15 | 77.4 |
| MS.gene58740.t1 | AT1G01300 | 24.036 | 441 | 253 | 18 | 15 | 438 | 105 | 480 | 1.23e-13 | 73.2 |
| MS.gene58740.t1 | AT3G12700 | 24.279 | 416 | 239 | 17 | 44 | 440 | 100 | 458 | 1.92e-13 | 72.4 |
| MS.gene58740.t1 | AT3G54400 | 23.040 | 421 | 227 | 15 | 40 | 438 | 75 | 420 | 7.58e-13 | 70.5 |
| MS.gene58740.t1 | AT5G36260 | 21.182 | 406 | 249 | 14 | 50 | 438 | 78 | 429 | 5.78e-11 | 64.7 |
Find 124 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGGTGTATTTAAGAAATT+TGG | 0.163767 | 8.1:-10097817 | None:intergenic |
| ATACTCAACTAGTATTAAAA+TGG | 0.205243 | 8.1:+10097218 | MS.gene58740:CDS |
| TGGTGGGGATCCATATAATT+TGG | 0.206282 | 8.1:+10097773 | MS.gene58740:CDS |
| AAGCAAGTTGTTCCCACTTT+AGG | 0.208826 | 8.1:+10098405 | MS.gene58740:CDS |
| CCTTGAACGCCAAATTTATC+TGG | 0.216108 | 8.1:-10097582 | None:intergenic |
| CAGGCCCAAGCCCATTCTTT+TGG | 0.218989 | 8.1:-10097737 | None:intergenic |
| AGGTGTCGGGTTTAGGTATA+TGG | 0.244141 | 8.1:-10097158 | None:intergenic |
| TTCAACCGAGTACTTTATTA+AGG | 0.255410 | 8.1:+10097887 | MS.gene58740:CDS |
| TCAGCAACTTCAATTATTAT+TGG | 0.283411 | 8.1:+10098299 | MS.gene58740:CDS |
| TTATTAGAGCCCAAATTATA+TGG | 0.286906 | 8.1:-10097783 | None:intergenic |
| AGCAAGTTGTTCCCACTTTA+GGG | 0.299048 | 8.1:+10098406 | MS.gene58740:CDS |
| TATTAAGGTGAAGTCAATTA+AGG | 0.299518 | 8.1:+10097902 | MS.gene58740:CDS |
| GATGTTGAATGGAGAATCTT+TGG | 0.313149 | 8.1:+10098197 | MS.gene58740:CDS |
| GAGATAGTGAAAGGAGAAAC+AGG | 0.320949 | 8.1:-10097095 | None:intergenic |
| CCAGATAAATTTGGCGTTCA+AGG | 0.327531 | 8.1:+10097582 | MS.gene58740:CDS |
| GGTGGGGATCCATATAATTT+GGG | 0.329517 | 8.1:+10097774 | MS.gene58740:CDS |
| GTGAAAGCTGTTGCACCATT+TGG | 0.338406 | 8.1:+10098095 | MS.gene58740:CDS |
| GTTGTAAGTTGTGGAGTTGT+AGG | 0.344427 | 8.1:-10097313 | None:intergenic |
| TGGTGCTAATTCAATGGTTA+AGG | 0.348036 | 8.1:+10098217 | MS.gene58740:CDS |
| TGCAATTGATTTGGTCTTGA+AGG | 0.357006 | 8.1:+10098172 | MS.gene58740:CDS |
| AACACAAAGGTGTCGGGTTT+AGG | 0.360622 | 8.1:-10097165 | None:intergenic |
| AAATCAATTGCAGGCACATT+TGG | 0.363244 | 8.1:-10098161 | None:intergenic |
| GGGCCTGGGAGTCTGTTTGT+TGG | 0.367528 | 8.1:+10097753 | MS.gene58740:CDS |
| TATGTCAATGACAAGATCTA+AGG | 0.376279 | 8.1:-10097256 | None:intergenic |
| GCATTTCTTTGTACCACATT+GGG | 0.378833 | 8.1:-10097340 | None:intergenic |
| ACAAGTGTTGTTTGTGCATC+CGG | 0.384973 | 8.1:-10097415 | None:intergenic |
| TCTTTGTACCACATTGGGTT+GGG | 0.385124 | 8.1:-10097335 | None:intergenic |
| CAAAAGAATGGGCTTGGGCC+TGG | 0.386990 | 8.1:+10097738 | MS.gene58740:CDS |
| GGCGTTCAAGGCTTTCTTCA+AGG | 0.387511 | 8.1:+10097594 | MS.gene58740:CDS |
| TAGATCTTGTCATTGACATA+AGG | 0.401290 | 8.1:+10097259 | MS.gene58740:CDS |
| ACTTCCCAAAAGAATGGGCT+TGG | 0.401719 | 8.1:+10097732 | MS.gene58740:CDS |
| ACCAATCATCCTTTCAAAAC+CGG | 0.404107 | 8.1:+10097396 | MS.gene58740:CDS |
| AAATGTGCCTGCAATTGATT+TGG | 0.406205 | 8.1:+10098163 | MS.gene58740:CDS |
| CCTTCAACTTCCCAAAAGAA+TGG | 0.409321 | 8.1:+10097726 | MS.gene58740:CDS |
| AATCTTTGGTGCTAATTCAA+TGG | 0.414472 | 8.1:+10098211 | MS.gene58740:CDS |
| TATTATTGGTGGGCATCAAT+TGG | 0.420251 | 8.1:+10098313 | MS.gene58740:CDS |
| CTGGGAGTCTGTTTGTTGGT+GGG | 0.420565 | 8.1:+10097757 | MS.gene58740:CDS |
| TGCATTTCTTTGTACCACAT+TGG | 0.420623 | 8.1:-10097341 | None:intergenic |
| ACAATTAGCAAGAGTGTTAC+CGG | 0.421303 | 8.1:+10098137 | MS.gene58740:CDS |
| AAAAGAATGGGCTTGGGCCT+GGG | 0.422617 | 8.1:+10097739 | MS.gene58740:CDS |
| TTCAAGACCAAATCAATTGC+AGG | 0.427026 | 8.1:-10098170 | None:intergenic |
| AGATCTTGTCATTGACATAA+GGG | 0.432329 | 8.1:+10097260 | MS.gene58740:CDS |
| TTCTTTGTACCACATTGGGT+TGG | 0.435161 | 8.1:-10097336 | None:intergenic |
| GATCTTGTTTCCTCAAAACT+TGG | 0.447946 | 8.1:+10098356 | MS.gene58740:CDS |
| TTAATAAAGTACTCGGTTGA+AGG | 0.448175 | 8.1:-10097885 | None:intergenic |
| ACAAGATCTAAGGGCACTGC+AGG | 0.448519 | 8.1:-10097246 | None:intergenic |
| CCTGGGAGTCTGTTTGTTGG+TGG | 0.450280 | 8.1:+10097756 | MS.gene58740:CDS |
| TTCACAAAAGCATTCAAAAG+TGG | 0.451413 | 8.1:-10098044 | None:intergenic |
| CTGCGACGGTTAGTAATAAG+AGG | 0.452299 | 8.1:-10097837 | None:intergenic |
| CTTCAACTTCCCAAAAGAAT+GGG | 0.455541 | 8.1:+10097727 | MS.gene58740:CDS |
| AATGAAAATGTGTTATGTCT+TGG | 0.457270 | 8.1:+10098242 | MS.gene58740:CDS |
| GAAGGAAAGTTATCAAATAT+AGG | 0.461915 | 8.1:-10097867 | None:intergenic |
| GTTGAACCGTTCAACCCATT+CGG | 0.462621 | 8.1:+10097441 | MS.gene58740:CDS |
| TACTCAACTAGTATTAAAAT+GGG | 0.463650 | 8.1:+10097219 | MS.gene58740:CDS |
| AGGAATCACTGTGCTGAGTT+TGG | 0.465877 | 8.1:-10097991 | None:intergenic |
| CGCGCACGTTGGTAACTCTT+CGG | 0.471565 | 8.1:-10097530 | None:intergenic |
| GCAACTTCAATTATTATTGG+TGG | 0.473806 | 8.1:+10098302 | MS.gene58740:CDS |
| TTGCTTTCTATTAACAAACT+AGG | 0.477513 | 8.1:+10097957 | MS.gene58740:CDS |
| TGTGTGTACCCAGATAAATT+TGG | 0.484387 | 8.1:+10097573 | MS.gene58740:CDS |
| CTTCCCAAAAGAATGGGCTT+GGG | 0.490626 | 8.1:+10097733 | MS.gene58740:CDS |
| GCAATTGATTTGGTCTTGAA+GGG | 0.492581 | 8.1:+10098173 | MS.gene58740:CDS |
| ATGGGAAACACAAAGGTGTC+GGG | 0.493132 | 8.1:-10097171 | None:intergenic |
| CAACTTCAATTATTATTGGT+GGG | 0.496037 | 8.1:+10098303 | MS.gene58740:CDS |
| ATTACTAACCGTCGCAGTAC+TGG | 0.497591 | 8.1:+10097843 | MS.gene58740:CDS |
| GAATCAAAACATGCTCCAAA+TGG | 0.497809 | 8.1:-10098110 | None:intergenic |
| GTGAAAGAAGAGATAGTGAA+AGG | 0.498488 | 8.1:-10097104 | None:intergenic |
| AGTCTTAAAATCCCCTAAAG+TGG | 0.503011 | 8.1:-10098418 | None:intergenic |
| TTCGGTGGATTCTTTGTGAG+TGG | 0.511303 | 8.1:+10097459 | MS.gene58740:CDS |
| ATTGGTACAATCAATGCAAC+CGG | 0.512419 | 8.1:-10097379 | None:intergenic |
| AATTGCAGGCACATTTGGTC+CGG | 0.513751 | 8.1:-10098156 | None:intergenic |
| AATCTAGTTGCGAATTGTGT+TGG | 0.514737 | 8.1:-10097672 | None:intergenic |
| TGGGAGTCTGTTTGTTGGTG+GGG | 0.521130 | 8.1:+10097758 | MS.gene58740:CDS |
| TTGGGTTGGGTTGTAAGTTG+TGG | 0.521537 | 8.1:-10097322 | None:intergenic |
| TGTCTTGGATTTGTTGATGC+GGG | 0.521708 | 8.1:+10098257 | MS.gene58740:CDS |
| GTAAACATAGTGTAAACTTA+CGG | 0.535570 | 8.1:-10097704 | None:intergenic |
| TGCAAGCAAGCTAGAGGCAC+CGG | 0.538614 | 8.1:+10097360 | MS.gene58740:CDS |
| TCTATTAACAAACTAGGAGA+AGG | 0.538655 | 8.1:+10097963 | MS.gene58740:CDS |
| TCACAAAGAATCCACCGAAT+GGG | 0.539095 | 8.1:-10097455 | None:intergenic |
| GTGAAAGGAGAAACAGGTGG+AGG | 0.539572 | 8.1:-10097089 | None:intergenic |
| GTCTTAAAATCCCCTAAAGT+GGG | 0.544065 | 8.1:-10098417 | None:intergenic |
| CTTGAACGCCAAATTTATCT+GGG | 0.544277 | 8.1:-10097581 | None:intergenic |
| ACATAAGGGAGCGTTTCCTA+TGG | 0.545597 | 8.1:+10097274 | MS.gene58740:CDS |
| CTTCACCTTAATAAAGTACT+CGG | 0.547033 | 8.1:-10097892 | None:intergenic |
| AATCATCACATTCAAACCAT+AGG | 0.553313 | 8.1:-10097290 | None:intergenic |
| TGATGCGGGTTCTGAGGAAG+TGG | 0.561699 | 8.1:+10098271 | MS.gene58740:CDS |
| GGTGTCGGGTTTAGGTATAT+GGG | 0.567463 | 8.1:-10097157 | None:intergenic |
| TGAAAGGAGAAACAGGTGGA+GGG | 0.570090 | 8.1:-10097088 | None:intergenic |
| CAATTGATTTGGTCTTGAAG+GGG | 0.571240 | 8.1:+10098174 | MS.gene58740:CDS |
| CCACCAACAAACAGACTCCC+AGG | 0.571791 | 8.1:-10097756 | None:intergenic |
| AATGGGAAACACAAAGGTGT+CGG | 0.575045 | 8.1:-10097172 | None:intergenic |
| TGCTTTGTTGAGTAAAAGTG+AGG | 0.581792 | 8.1:-10098387 | None:intergenic |
| ATAATAATTGAAGTTGCTGA+TGG | 0.586341 | 8.1:-10098296 | None:intergenic |
| CTTTCTTCAAGGTCTAAGCA+AGG | 0.587331 | 8.1:+10097605 | MS.gene58740:CDS |
| ATGTCAATGACAAGATCTAA+GGG | 0.588940 | 8.1:-10097255 | None:intergenic |
| AATCCACCGAATGGGTTGAA+CGG | 0.593083 | 8.1:-10097447 | None:intergenic |
| CGGCCATCGCTAGTGATACG+TGG | 0.593354 | 8.1:-10097510 | None:intergenic |
| GAACCGTTCAACCCATTCGG+TGG | 0.602812 | 8.1:+10097444 | MS.gene58740:CDS |
| TTTCTTCAAGGTCTAAGCAA+GGG | 0.612379 | 8.1:+10097606 | MS.gene58740:CDS |
| CTCACAAAGAATCCACCGAA+TGG | 0.614046 | 8.1:-10097456 | None:intergenic |
| ATCGCTAGTGATACGTGGGA+AGG | 0.614330 | 8.1:-10097505 | None:intergenic |
| CAAGATCTAAGGGCACTGCA+GGG | 0.615487 | 8.1:-10097245 | None:intergenic |
| ATGTCTTGGATTTGTTGATG+CGG | 0.620309 | 8.1:+10098256 | MS.gene58740:CDS |
| AAGATCTAAGGGCACTGCAG+GGG | 0.622294 | 8.1:-10097244 | None:intergenic |
| AAGAAATGCAAGCAAGCTAG+AGG | 0.624549 | 8.1:+10097354 | MS.gene58740:CDS |
| ATTAACAAACTAGGAGAAGG+TGG | 0.633136 | 8.1:+10097966 | MS.gene58740:CDS |
| TGCGACGGTTAGTAATAAGA+GGG | 0.636681 | 8.1:-10097836 | None:intergenic |
| GAAACGTGGCACGCGCACGT+TGG | 0.639318 | 8.1:-10097541 | None:intergenic |
| TTCCCACGTATCACTAGCGA+TGG | 0.641396 | 8.1:+10097507 | MS.gene58740:CDS |
| TGTGAGTGGAGACGTAGGAG+AGG | 0.641528 | 8.1:+10097473 | MS.gene58740:CDS |
| ATTTGTTGATGCGGGTTCTG+AGG | 0.643464 | 8.1:+10098265 | MS.gene58740:CDS |
| AATTGATTTGGTCTTGAAGG+GGG | 0.646279 | 8.1:+10098175 | MS.gene58740:CDS |
| GGCCATCGCTAGTGATACGT+GGG | 0.647935 | 8.1:-10097509 | None:intergenic |
| TTCTTTGTGAGTGGAGACGT+AGG | 0.651013 | 8.1:+10097468 | MS.gene58740:CDS |
| TCAATTGGAAGATAATCTTG+TGG | 0.652504 | 8.1:+10098328 | MS.gene58740:CDS |
| GCAAGTTGTTCCCACTTTAG+GGG | 0.655905 | 8.1:+10098407 | MS.gene58740:CDS |
| ATATAGGACCAGTACTGCGA+CGG | 0.671660 | 8.1:-10097851 | None:intergenic |
| ATAGTGAAAGGAGAAACAGG+TGG | 0.675322 | 8.1:-10097092 | None:intergenic |
| ACTTACAACCCAACCCAATG+TGG | 0.685608 | 8.1:+10097327 | MS.gene58740:CDS |
| GATGCGGGTTCTGAGGAAGT+GGG | 0.693306 | 8.1:+10098272 | MS.gene58740:CDS |
| GGATGCACAAACAACACTTG+TGG | 0.695025 | 8.1:+10097417 | MS.gene58740:CDS |
| GAAGTGTGTAATGTTGTGTG+AGG | 0.719466 | 8.1:-10098011 | None:intergenic |
| GCGACGGTTAGTAATAAGAG+GGG | 0.739886 | 8.1:-10097835 | None:intergenic |
| ACACATGATGAAATGAAACG+TGG | 0.754331 | 8.1:-10097555 | None:intergenic |
| TTTGTTAATAGAAAGCAACG+TGG | 0.831722 | 8.1:-10097952 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTCAACTAGTATTAAAA+TGG | + | chr8.1:10097218-10097237 | MS.gene58740:CDS | 20.0% |
| !! | TACTCAACTAGTATTAAAAT+GGG | + | chr8.1:10097219-10097238 | MS.gene58740:CDS | 20.0% |
| ! | AATGAAAATGTGTTATGTCT+TGG | + | chr8.1:10098242-10098261 | MS.gene58740:CDS | 25.0% |
| ! | ATAATAATTGAAGTTGCTGA+TGG | - | chr8.1:10098299-10098318 | None:intergenic | 25.0% |
| ! | CAACTTCAATTATTATTGGT+GGG | + | chr8.1:10098303-10098322 | MS.gene58740:CDS | 25.0% |
| ! | GAAGGAAAGTTATCAAATAT+AGG | - | chr8.1:10097870-10097889 | None:intergenic | 25.0% |
| ! | GTAAACATAGTGTAAACTTA+CGG | - | chr8.1:10097707-10097726 | None:intergenic | 25.0% |
| ! | TATTAAGGTGAAGTCAATTA+AGG | + | chr8.1:10097902-10097921 | MS.gene58740:CDS | 25.0% |
| ! | TCAGCAACTTCAATTATTAT+TGG | + | chr8.1:10098299-10098318 | MS.gene58740:CDS | 25.0% |
| ! | TTATTAGAGCCCAAATTATA+TGG | - | chr8.1:10097786-10097805 | None:intergenic | 25.0% |
| ! | TTGCTTTCTATTAACAAACT+AGG | + | chr8.1:10097957-10097976 | MS.gene58740:CDS | 25.0% |
| AATCATCACATTCAAACCAT+AGG | - | chr8.1:10097293-10097312 | None:intergenic | 30.0% | |
| AGATCTTGTCATTGACATAA+GGG | + | chr8.1:10097260-10097279 | MS.gene58740:CDS | 30.0% | |
| ATGTCAATGACAAGATCTAA+GGG | - | chr8.1:10097258-10097277 | None:intergenic | 30.0% | |
| GCAACTTCAATTATTATTGG+TGG | + | chr8.1:10098302-10098321 | MS.gene58740:CDS | 30.0% | |
| TAGATCTTGTCATTGACATA+AGG | + | chr8.1:10097259-10097278 | MS.gene58740:CDS | 30.0% | |
| TATGTCAATGACAAGATCTA+AGG | - | chr8.1:10097259-10097278 | None:intergenic | 30.0% | |
| TCAATTGGAAGATAATCTTG+TGG | + | chr8.1:10098328-10098347 | MS.gene58740:CDS | 30.0% | |
| TCTATTAACAAACTAGGAGA+AGG | + | chr8.1:10097963-10097982 | MS.gene58740:CDS | 30.0% | |
| TTCAACCGAGTACTTTATTA+AGG | + | chr8.1:10097887-10097906 | MS.gene58740:CDS | 30.0% | |
| TTTGTTAATAGAAAGCAACG+TGG | - | chr8.1:10097955-10097974 | None:intergenic | 30.0% | |
| ! | AGGGGTGTATTTAAGAAATT+TGG | - | chr8.1:10097820-10097839 | None:intergenic | 30.0% |
| ! | CTTCACCTTAATAAAGTACT+CGG | - | chr8.1:10097895-10097914 | None:intergenic | 30.0% |
| ! | TTAATAAAGTACTCGGTTGA+AGG | - | chr8.1:10097888-10097907 | None:intergenic | 30.0% |
| !! | AATCTTTGGTGCTAATTCAA+TGG | + | chr8.1:10098211-10098230 | MS.gene58740:CDS | 30.0% |
| !! | TTCACAAAAGCATTCAAAAG+TGG | - | chr8.1:10098047-10098066 | None:intergenic | 30.0% |
| !!! | TTGAGTATTGAAGCGTTTTT+GGG | - | chr8.1:10097206-10097225 | None:intergenic | 30.0% |
| AAATCAATTGCAGGCACATT+TGG | - | chr8.1:10098164-10098183 | None:intergenic | 35.0% | |
| AAATGTGCCTGCAATTGATT+TGG | + | chr8.1:10098163-10098182 | MS.gene58740:CDS | 35.0% | |
| AATCTAGTTGCGAATTGTGT+TGG | - | chr8.1:10097675-10097694 | None:intergenic | 35.0% | |
| ACACATGATGAAATGAAACG+TGG | - | chr8.1:10097558-10097577 | None:intergenic | 35.0% | |
| ACCAATCATCCTTTCAAAAC+CGG | + | chr8.1:10097396-10097415 | MS.gene58740:CDS | 35.0% | |
| ATTAACAAACTAGGAGAAGG+TGG | + | chr8.1:10097966-10097985 | MS.gene58740:CDS | 35.0% | |
| ATTGGTACAATCAATGCAAC+CGG | - | chr8.1:10097382-10097401 | None:intergenic | 35.0% | |
| CTTCAACTTCCCAAAAGAAT+GGG | + | chr8.1:10097727-10097746 | MS.gene58740:CDS | 35.0% | |
| CTTGAACGCCAAATTTATCT+GGG | - | chr8.1:10097584-10097603 | None:intergenic | 35.0% | |
| GAATCAAAACATGCTCCAAA+TGG | - | chr8.1:10098113-10098132 | None:intergenic | 35.0% | |
| GATGTTGAATGGAGAATCTT+TGG | + | chr8.1:10098197-10098216 | MS.gene58740:CDS | 35.0% | |
| GCATTTCTTTGTACCACATT+GGG | - | chr8.1:10097343-10097362 | None:intergenic | 35.0% | |
| GTGAAAGAAGAGATAGTGAA+AGG | - | chr8.1:10097107-10097126 | None:intergenic | 35.0% | |
| TGCATTTCTTTGTACCACAT+TGG | - | chr8.1:10097344-10097363 | None:intergenic | 35.0% | |
| TGTGTGTACCCAGATAAATT+TGG | + | chr8.1:10097573-10097592 | MS.gene58740:CDS | 35.0% | |
| TTCAAGACCAAATCAATTGC+AGG | - | chr8.1:10098173-10098192 | None:intergenic | 35.0% | |
| TTTCTTCAAGGTCTAAGCAA+GGG | + | chr8.1:10097606-10097625 | MS.gene58740:CDS | 35.0% | |
| ! | GATCTTGTTTCCTCAAAACT+TGG | + | chr8.1:10098356-10098375 | MS.gene58740:CDS | 35.0% |
| ! | TATTATTGGTGGGCATCAAT+TGG | + | chr8.1:10098313-10098332 | MS.gene58740:CDS | 35.0% |
| ! | TGCTTTGTTGAGTAAAAGTG+AGG | - | chr8.1:10098390-10098409 | None:intergenic | 35.0% |
| ! | TGGTGCTAATTCAATGGTTA+AGG | + | chr8.1:10098217-10098236 | MS.gene58740:CDS | 35.0% |
| ! | TTTTGCAATGGGAAACACAA+AGG | - | chr8.1:10097181-10097200 | None:intergenic | 35.0% |
| !! | AAGGGTAAAAAAGGAGTGTT+AGG | + | chr8.1:10097624-10097643 | MS.gene58740:CDS | 35.0% |
| !! | AATTGATTTGGTCTTGAAGG+GGG | + | chr8.1:10098175-10098194 | MS.gene58740:CDS | 35.0% |
| !! | ACAATTAGCAAGAGTGTTAC+CGG | + | chr8.1:10098137-10098156 | MS.gene58740:CDS | 35.0% |
| !! | ATGTCTTGGATTTGTTGATG+CGG | + | chr8.1:10098256-10098275 | MS.gene58740:CDS | 35.0% |
| !! | CAATTGATTTGGTCTTGAAG+GGG | + | chr8.1:10098174-10098193 | MS.gene58740:CDS | 35.0% |
| !! | GCAATTGATTTGGTCTTGAA+GGG | + | chr8.1:10098173-10098192 | MS.gene58740:CDS | 35.0% |
| !! | TGCAATTGATTTGGTCTTGA+AGG | + | chr8.1:10098172-10098191 | MS.gene58740:CDS | 35.0% |
| !!! | GTTGAGTATTGAAGCGTTTT+TGG | - | chr8.1:10097207-10097226 | None:intergenic | 35.0% |
| !!! | GTTTTTGGGTCTTTTGCAAT+GGG | - | chr8.1:10097192-10097211 | None:intergenic | 35.0% |
| AAGAAATGCAAGCAAGCTAG+AGG | + | chr8.1:10097354-10097373 | MS.gene58740:CDS | 40.0% | |
| AAGCAAGTTGTTCCCACTTT+AGG | + | chr8.1:10098405-10098424 | MS.gene58740:CDS | 40.0% | |
| AGCAAGTTGTTCCCACTTTA+GGG | + | chr8.1:10098406-10098425 | MS.gene58740:CDS | 40.0% | |
| ATAGTGAAAGGAGAAACAGG+TGG | - | chr8.1:10097095-10097114 | None:intergenic | 40.0% | |
| CCAGATAAATTTGGCGTTCA+AGG | + | chr8.1:10097582-10097601 | MS.gene58740:CDS | 40.0% | |
| CCTTCAACTTCCCAAAAGAA+TGG | + | chr8.1:10097726-10097745 | MS.gene58740:CDS | 40.0% | |
| CCTTGAACGCCAAATTTATC+TGG | - | chr8.1:10097585-10097604 | None:intergenic | 40.0% | |
| CTTTCTTCAAGGTCTAAGCA+AGG | + | chr8.1:10097605-10097624 | MS.gene58740:CDS | 40.0% | |
| GAAGTGTGTAATGTTGTGTG+AGG | - | chr8.1:10098014-10098033 | None:intergenic | 40.0% | |
| GAGATAGTGAAAGGAGAAAC+AGG | - | chr8.1:10097098-10097117 | None:intergenic | 40.0% | |
| GGTCTAAGCAAGGGTAAAAA+AGG | + | chr8.1:10097615-10097634 | MS.gene58740:CDS | 40.0% | |
| GGTGGGGATCCATATAATTT+GGG | + | chr8.1:10097774-10097793 | MS.gene58740:CDS | 40.0% | |
| GTTGTAAGTTGTGGAGTTGT+AGG | - | chr8.1:10097316-10097335 | None:intergenic | 40.0% | |
| TCACAAAGAATCCACCGAAT+GGG | - | chr8.1:10097458-10097477 | None:intergenic | 40.0% | |
| TCTTTGTACCACATTGGGTT+GGG | - | chr8.1:10097338-10097357 | None:intergenic | 40.0% | |
| TGCGACGGTTAGTAATAAGA+GGG | - | chr8.1:10097839-10097858 | None:intergenic | 40.0% | |
| TGGTGGGGATCCATATAATT+TGG | + | chr8.1:10097773-10097792 | MS.gene58740:CDS | 40.0% | |
| TTCTTTGTACCACATTGGGT+TGG | - | chr8.1:10097339-10097358 | None:intergenic | 40.0% | |
| ! | AATGGGAAACACAAAGGTGT+CGG | - | chr8.1:10097175-10097194 | None:intergenic | 40.0% |
| !! | ACAAGTGTTGTTTGTGCATC+CGG | - | chr8.1:10097418-10097437 | None:intergenic | 40.0% |
| !! | CCATTCTTTTGGGAAGTTGA+AGG | - | chr8.1:10097729-10097748 | None:intergenic | 40.0% |
| !! | TGTCTTGGATTTGTTGATGC+GGG | + | chr8.1:10098257-10098276 | MS.gene58740:CDS | 40.0% |
| !!! | CGTTTTTGGGTCTTTTGCAA+TGG | - | chr8.1:10097193-10097212 | None:intergenic | 40.0% |
| !!! | TCCGGTTTTGAAAGGATGAT+TGG | - | chr8.1:10097400-10097419 | None:intergenic | 40.0% |
| !!! | TTTGTGCATCCGGTTTTGAA+AGG | - | chr8.1:10097408-10097427 | None:intergenic | 40.0% |
| AATTGCAGGCACATTTGGTC+CGG | - | chr8.1:10098159-10098178 | None:intergenic | 45.0% | |
| ACTTACAACCCAACCCAATG+TGG | + | chr8.1:10097327-10097346 | MS.gene58740:CDS | 45.0% | |
| AGGAATCACTGTGCTGAGTT+TGG | - | chr8.1:10097994-10098013 | None:intergenic | 45.0% | |
| ATATAGGACCAGTACTGCGA+CGG | - | chr8.1:10097854-10097873 | None:intergenic | 45.0% | |
| ATTACTAACCGTCGCAGTAC+TGG | + | chr8.1:10097843-10097862 | MS.gene58740:CDS | 45.0% | |
| CTCACAAAGAATCCACCGAA+TGG | - | chr8.1:10097459-10097478 | None:intergenic | 45.0% | |
| CTGCGACGGTTAGTAATAAG+AGG | - | chr8.1:10097840-10097859 | None:intergenic | 45.0% | |
| GCAAGTTGTTCCCACTTTAG+GGG | + | chr8.1:10098407-10098426 | MS.gene58740:CDS | 45.0% | |
| GCGACGGTTAGTAATAAGAG+GGG | - | chr8.1:10097838-10097857 | None:intergenic | 45.0% | |
| GGATGCACAAACAACACTTG+TGG | + | chr8.1:10097417-10097436 | MS.gene58740:CDS | 45.0% | |
| GTGAAAGCTGTTGCACCATT+TGG | + | chr8.1:10098095-10098114 | MS.gene58740:CDS | 45.0% | |
| GTTGAACCGTTCAACCCATT+CGG | + | chr8.1:10097441-10097460 | MS.gene58740:CDS | 45.0% | |
| TCTTGAAGGGGGATGTTGAA+TGG | + | chr8.1:10098186-10098205 | MS.gene58740:CDS | 45.0% | |
| TGAAAGGAGAAACAGGTGGA+GGG | - | chr8.1:10097091-10097110 | None:intergenic | 45.0% | |
| TTCTTTGTGAGTGGAGACGT+AGG | + | chr8.1:10097468-10097487 | MS.gene58740:CDS | 45.0% | |
| ! | ACTTCCCAAAAGAATGGGCT+TGG | + | chr8.1:10097732-10097751 | MS.gene58740:CDS | 45.0% |
| ! | ATTTGTTGATGCGGGTTCTG+AGG | + | chr8.1:10098265-10098284 | MS.gene58740:CDS | 45.0% |
| ! | CTTCCCAAAAGAATGGGCTT+GGG | + | chr8.1:10097733-10097752 | MS.gene58740:CDS | 45.0% |
| ! | GGAGCTAAAGCCAAGTTTTG+AGG | - | chr8.1:10098369-10098388 | None:intergenic | 45.0% |
| ! | GGTGTCGGGTTTAGGTATAT+GGG | - | chr8.1:10097160-10097179 | None:intergenic | 45.0% |
| ! | TTCGGTGGATTCTTTGTGAG+TGG | + | chr8.1:10097459-10097478 | MS.gene58740:CDS | 45.0% |
| !! | AACACAAAGGTGTCGGGTTT+AGG | - | chr8.1:10097168-10097187 | None:intergenic | 45.0% |
| !! | AATCCACCGAATGGGTTGAA+CGG | - | chr8.1:10097450-10097469 | None:intergenic | 45.0% |
| !! | ACATAAGGGAGCGTTTCCTA+TGG | + | chr8.1:10097274-10097293 | MS.gene58740:CDS | 45.0% |
| !! | AGGTGTCGGGTTTAGGTATA+TGG | - | chr8.1:10097161-10097180 | None:intergenic | 45.0% |
| !! | ATGGGAAACACAAAGGTGTC+GGG | - | chr8.1:10097174-10097193 | None:intergenic | 45.0% |
| !! | TTGGGTTGGGTTGTAAGTTG+TGG | - | chr8.1:10097325-10097344 | None:intergenic | 45.0% |
| ATCGCTAGTGATACGTGGGA+AGG | - | chr8.1:10097508-10097527 | None:intergenic | 50.0% | |
| GGCGTTCAAGGCTTTCTTCA+AGG | + | chr8.1:10097594-10097613 | MS.gene58740:CDS | 50.0% | |
| GTGAAAGGAGAAACAGGTGG+AGG | - | chr8.1:10097092-10097111 | None:intergenic | 50.0% | |
| TTCCCACGTATCACTAGCGA+TGG | + | chr8.1:10097507-10097526 | MS.gene58740:CDS | 50.0% | |
| ! | AAAAGAATGGGCTTGGGCCT+GGG | + | chr8.1:10097739-10097758 | MS.gene58740:CDS | 50.0% |
| ! | AGGCCCAAGCCCATTCTTTT+GGG | - | chr8.1:10097739-10097758 | None:intergenic | 50.0% |
| ! | CTGGGAGTCTGTTTGTTGGT+GGG | + | chr8.1:10097757-10097776 | MS.gene58740:CDS | 50.0% |
| ! | TGGGAGTCTGTTTGTTGGTG+GGG | + | chr8.1:10097758-10097777 | MS.gene58740:CDS | 50.0% |
| !! | AAGATCTAAGGGCACTGCAG+GGG | - | chr8.1:10097247-10097266 | None:intergenic | 50.0% |
| !! | ACAAGATCTAAGGGCACTGC+AGG | - | chr8.1:10097249-10097268 | None:intergenic | 50.0% |
| !! | CAAGATCTAAGGGCACTGCA+GGG | - | chr8.1:10097248-10097267 | None:intergenic | 50.0% |
| CCACCAACAAACAGACTCCC+AGG | - | chr8.1:10097759-10097778 | None:intergenic | 55.0% | |
| GAACCGTTCAACCCATTCGG+TGG | + | chr8.1:10097444-10097463 | MS.gene58740:CDS | 55.0% | |
| GGCCATCGCTAGTGATACGT+GGG | - | chr8.1:10097512-10097531 | None:intergenic | 55.0% | |
| TGTGAGTGGAGACGTAGGAG+AGG | + | chr8.1:10097473-10097492 | MS.gene58740:CDS | 55.0% | |
| ! | CAAAAGAATGGGCTTGGGCC+TGG | + | chr8.1:10097738-10097757 | MS.gene58740:CDS | 55.0% |
| ! | CAGGCCCAAGCCCATTCTTT+TGG | - | chr8.1:10097740-10097759 | None:intergenic | 55.0% |
| ! | CCTGGGAGTCTGTTTGTTGG+TGG | + | chr8.1:10097756-10097775 | MS.gene58740:CDS | 55.0% |
| ! | TGCAAGCAAGCTAGAGGCAC+CGG | + | chr8.1:10097360-10097379 | MS.gene58740:CDS | 55.0% |
| !! | CGCGCACGTTGGTAACTCTT+CGG | - | chr8.1:10097533-10097552 | None:intergenic | 55.0% |
| !! | GATGCGGGTTCTGAGGAAGT+GGG | + | chr8.1:10098272-10098291 | MS.gene58740:CDS | 55.0% |
| !! | TGATGCGGGTTCTGAGGAAG+TGG | + | chr8.1:10098271-10098290 | MS.gene58740:CDS | 55.0% |
| CGGCCATCGCTAGTGATACG+TGG | - | chr8.1:10097513-10097532 | None:intergenic | 60.0% | |
| GGGCCTGGGAGTCTGTTTGT+TGG | + | chr8.1:10097753-10097772 | MS.gene58740:CDS | 60.0% | |
| GAAACGTGGCACGCGCACGT+TGG | - | chr8.1:10097544-10097563 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10097072 | 10098436 | 10097072 | ID=MS.gene58740 |
| chr8.1 | mRNA | 10097072 | 10098436 | 10097072 | ID=MS.gene58740.t1;Parent=MS.gene58740 |
| chr8.1 | exon | 10097072 | 10098436 | 10097072 | ID=MS.gene58740.t1.exon1;Parent=MS.gene58740.t1 |
| chr8.1 | CDS | 10097072 | 10098436 | 10097072 | ID=cds.MS.gene58740.t1;Parent=MS.gene58740.t1 |
| Gene Sequence |
| Protein sequence |
