Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58982.t1 | PNX75878.1 | 73 | 63 | 17 | 0 | 1 | 63 | 28 | 90 | 3.30E-14 | 87 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58982.t1 | A0A2K3LBG3 | 73.0 | 63 | 17 | 0 | 1 | 63 | 28 | 90 | 2.4e-14 | 87.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene58982 | 0.812693 | 3.73E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene58982 | 0.807874 | 4.08E-50 | -1.69E-46 |
| MS.gene050323 | MS.gene58982 | 0.801147 | 1.03E-48 | -1.69E-46 |
| MS.gene050496 | MS.gene58982 | 0.810019 | 1.42E-50 | -1.69E-46 |
| MS.gene050697 | MS.gene58982 | 0.802751 | 4.84E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene58982 | 0.826848 | 2.17E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene58982 | 0.830628 | 2.64E-55 | -1.69E-46 |
| MS.gene051509 | MS.gene58982 | 0.804981 | 1.66E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene58982 | 0.817269 | 3.60E-52 | -1.69E-46 |
| MS.gene053626 | MS.gene58982 | 0.814694 | 1.35E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene58982 | 0.804267 | 2.34E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene58982 | 0.80138 | 9.25E-49 | -1.69E-46 |
| MS.gene055911 | MS.gene58982 | 0.802365 | 5.81E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene58982 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene58982 | 0.809146 | 2.19E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene58982 | 0.804496 | 2.10E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene58982 | 0.810091 | 1.37E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene58982 | 0.816417 | 5.59E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene58982 | -0.801601 | 8.34E-49 | -1.69E-46 |
| MS.gene058173 | MS.gene58982 | -0.80569 | 1.18E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene58982 | -0.802034 | 6.80E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene58982 | 0.808951 | 2.41E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene58982 | 0.820538 | 6.51E-53 | -1.69E-46 |
| MS.gene059368 | MS.gene58982 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene059680 | MS.gene58982 | 0.803995 | 2.67E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene58982 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene58982 | 0.828714 | 7.71E-55 | -1.69E-46 |
| MS.gene060187 | MS.gene58982 | -0.803354 | 3.63E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene58982 | -0.81984 | 9.40E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene58982 | 0.853138 | 2.91E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 3.2:+44427976 | None:intergenic |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 3.2:+44427962 | None:intergenic |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 3.2:+44427940 | None:intergenic |
| TGCGTGCCACCAGGAACTTC+TGG | 0.331803 | 3.2:-44428002 | MS.gene58982:CDS |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 3.2:-44428055 | MS.gene58982:CDS |
| TCTTGGTTACCAGAAGTTCC+TGG | 0.400394 | 3.2:+44427993 | None:intergenic |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 3.2:+44427949 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 3.2:+44427953 | None:intergenic |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 3.2:+44428045 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 3.2:+44428066 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 3.2:-44428047 | MS.gene58982:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 3.2:+44427941 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 3.2:+44427944 | None:intergenic |
| TGATGATTCAACTCAATTCA+AGG | 0.508747 | 3.2:+44427918 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+AGG | 0.530335 | 3.2:-44428011 | MS.gene58982:CDS |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 3.2:+44427963 | None:intergenic |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 3.2:-44428091 | MS.gene58982:CDS |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 3.2:-44428107 | None:intergenic |
| GATGATTCAACTCAATTCAA+GGG | 0.567646 | 3.2:+44427919 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 3.2:+44428050 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 3.2:-44428031 | MS.gene58982:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 3.2:-44428054 | MS.gene58982:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 3.2:-44428073 | MS.gene58982:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 3.2:-44427957 | MS.gene58982:CDS |
| TGGTTACCAGAAGTTCCTGG+TGG | 0.663688 | 3.2:+44427996 | None:intergenic |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 3.2:-44427954 | MS.gene58982:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 3.2:+44428039 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | + | chr3.2:44428046-44428065 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | chr3.2:44428029-44428048 | None:intergenic | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr3.2:44427946-44427965 | MS.gene58982:CDS | 45.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr3.2:44428062-44428081 | MS.gene58982:CDS | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | chr3.2:44428026-44428045 | None:intergenic | 50.0% | |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr3.2:44427988-44428007 | MS.gene58982:CDS | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr3.2:44428059-44428078 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr3.2:44427983-44428002 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr3.2:44427928-44427947 | MS.gene58982:CDS | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr3.2:44428060-44428079 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr3.2:44428081-44428100 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr3.2:44428065-44428084 | MS.gene58982:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr3.2:44427956-44427975 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr3.2:44428073-44428092 | None:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | chr3.2:44428017-44428036 | MS.gene58982:CDS | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr3.2:44427977-44427996 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr3.2:44428069-44428088 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr3.2:44428078-44428097 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr3.2:44428082-44428101 | None:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr3.2:44427972-44427991 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr3.2:44427972-44427991 | MS.gene58982:CDS | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | chr3.2:44428008-44428027 | MS.gene58982:CDS | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr3.2:44427965-44427984 | MS.gene58982:CDS | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr3.2:44427964-44427983 | MS.gene58982:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 44427925 | 44428116 | 44427925 | ID=MS.gene58982 |
| chr3.2 | mRNA | 44427925 | 44428116 | 44427925 | ID=MS.gene58982.t1;Parent=MS.gene58982 |
| chr3.2 | exon | 44427925 | 44428116 | 44427925 | ID=MS.gene58982.t1.exon1;Parent=MS.gene58982.t1 |
| chr3.2 | CDS | 44427925 | 44428116 | 44427925 | ID=cds.MS.gene58982.t1;Parent=MS.gene58982.t1 |
| Gene Sequence |
| Protein sequence |
