AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60172.t1 MTR_4g097540 99.577 473 2 0 1 473 1 473 0.0 985
MS.gene60172.t1 MTR_5g015020 83.402 482 67 5 1 471 1 480 0.0 835
MS.gene60172.t1 MTR_3g103550 70.139 432 122 3 3 433 1 426 0.0 657
MS.gene60172.t1 MTR_6g091760 60.325 431 161 4 4 434 14 434 0.0 554
MS.gene60172.t1 MTR_8g098930 55.876 451 187 4 2 450 10 450 0.0 552
MS.gene60172.t1 MTR_8g101750 58.352 437 164 5 4 439 12 431 0.0 535
MS.gene60172.t1 MTR_8g028600 53.759 439 189 3 2 438 9 435 0.0 520
MS.gene60172.t1 MTR_7g079080 53.103 435 191 3 2 434 9 432 6.70e-178 507
MS.gene60172.t1 MTR_8g101820 54.233 437 169 6 4 439 12 418 4.74e-170 485
MS.gene60172.t1 MTR_6g463260 45.517 435 225 5 4 437 104 527 1.54e-146 429
MS.gene60172.t1 MTR_7g079080 52.959 338 146 3 99 434 1 327 9.55e-135 394
MS.gene60172.t1 MTR_5g011400 41.687 415 232 3 16 430 84 488 9.38e-126 375
MS.gene60172.t1 MTR_2g090730 81.720 93 13 1 57 149 12 100 4.07e-49 167
MS.gene60172.t1 MTR_5g033230 25.410 244 162 9 24 253 35 272 1.86e-14 75.9
MS.gene60172.t1 MTR_5g033230 26.966 178 122 6 79 253 100 272 2.53e-14 75.5
MS.gene60172.t1 MTR_8g023140 22.449 343 235 13 112 433 182 514 4.08e-13 71.6
MS.gene60172.t1 MTR_4g011820 22.802 364 228 14 79 431 61 382 7.53e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60172.t1 AT4G37770 71.698 477 123 4 1 473 1 469 0.0 715
MS.gene60172.t1 AT3G49700 74.153 472 109 4 1 466 1 465 0.0 712
MS.gene60172.t1 AT5G65800 73.517 472 112 5 1 466 1 465 0.0 704
MS.gene60172.t1 AT2G22810 69.023 481 134 3 1 473 1 474 0.0 691
MS.gene60172.t1 AT4G08040 66.450 462 145 3 3 463 1 453 0.0 651
MS.gene60172.t1 AT4G26200 58.333 432 171 3 4 434 18 441 0.0 534
MS.gene60172.t1 AT3G61510 54.147 434 187 3 2 435 9 430 0.0 520
MS.gene60172.t1 AT1G01480 51.380 471 213 4 3 464 11 474 1.72e-179 512
MS.gene60172.t1 AT4G11280 53.118 433 194 2 1 433 12 435 9.68e-177 505
MS.gene60172.t1 AT1G01480 51.467 375 166 4 99 464 1 368 2.60e-140 408
MS.gene60172.t1 AT5G51690 43.590 429 231 4 4 431 69 487 3.06e-139 409
MS.gene60172.t1 AT1G62960 42.227 431 231 8 4 431 132 547 3.97e-126 378
MS.gene60172.t1 AT5G51690 42.932 382 200 4 50 431 1 364 9.32e-121 358
MS.gene60172.t1 AT1G23310 23.868 243 167 8 24 253 35 272 7.16e-15 77.0
MS.gene60172.t1 AT1G23310 23.868 243 167 8 24 253 35 272 1.20e-14 76.6
MS.gene60172.t1 AT1G70580 23.457 243 168 8 24 253 35 272 3.43e-14 75.1
MS.gene60172.t1 AT1G70580 23.457 243 168 8 24 253 35 272 3.43e-14 75.1
MS.gene60172.t1 AT1G70580 23.457 243 168 8 24 253 35 272 3.43e-14 75.1
MS.gene60172.t1 AT1G70580 23.457 243 168 8 24 253 35 272 3.43e-14 75.1
MS.gene60172.t1 AT2G22250 22.222 270 182 7 105 369 106 352 4.53e-13 71.2
MS.gene60172.t1 AT1G77670 24.267 375 237 16 71 431 92 433 1.07e-12 70.1
MS.gene60172.t1 AT2G24850 23.724 333 211 12 114 433 117 419 1.06e-11 67.0
MS.gene60172.t1 AT2G20610 23.333 360 227 14 88 433 111 435 2.94e-11 65.9
MS.gene60172.t1 AT4G23600 23.118 372 244 13 87 444 78 421 4.10e-11 65.1

Find 91 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTACTCCTTTGACTTTA+AGG 0.007055 8.2:+24538427 None:intergenic
TCCATGACGCTGATGAAATT+TGG 0.143831 8.2:+24538270 None:intergenic
CGTCAGAACAAGCTTGTTTC+GGG 0.214801 8.2:-24537970 MS.gene60172:CDS
ACAAATATTATCAGCGTCTT+TGG 0.227556 8.2:+24538215 None:intergenic
CAAGACTCTTCATACTTCTT+AGG 0.254225 8.2:-24539167 MS.gene60172:CDS
GAGAGCAAGTTCACGGAATA+TGG 0.287004 8.2:+24538879 None:intergenic
GTTACTTACCCTGGATAATA+AGG 0.296953 8.2:+24538673 None:intergenic
ACCTGGCAAACCCAAGTCTT+TGG 0.309354 8.2:+24538164 None:intergenic
CAACACATTTGAAGCTGAAA+TGG 0.312708 8.2:-24537866 MS.gene60172:CDS
ACGTCAGAACAAGCTTGTTT+CGG 0.332943 8.2:-24537971 MS.gene60172:CDS
ACAAGCATTGGTAGATTTCA+TGG 0.337094 8.2:-24538824 MS.gene60172:CDS
GCTTTCCAAGCAGCTAATTC+CGG 0.349870 8.2:+24537936 None:intergenic
CCCATTTGAATAATTCCTTT+AGG 0.350296 8.2:+24539101 None:intergenic
TTCGGTTACTTGAAAGTTGT+TGG 0.352316 8.2:+24538491 None:intergenic
CTTCCTACTCCTTATTATCC+AGG 0.362035 8.2:-24538682 MS.gene60172:CDS
GAGTCCACTGGCAATGTTAA+TGG 0.369447 8.2:-24537688 MS.gene60172:CDS
GAGGAATGCTTCTCCTTTCT+CGG 0.379843 8.2:+24538705 None:intergenic
TCCTAAAGGAATTATTCAAA+TGG 0.384178 8.2:-24539102 MS.gene60172:CDS
TCCAAAGACTTGGGTTTGCC+AGG 0.387995 8.2:-24538165 MS.gene60172:CDS
TTGGAGCTTGTGCAATGTAT+TGG 0.402052 8.2:+24538510 None:intergenic
GTGGTGCCTAGCGGGTTTGA+TGG 0.413274 8.2:+24538396 None:intergenic
TTGAAGCTGAAATGGAACTA+TGG 0.421415 8.2:-24537858 MS.gene60172:CDS
CATTTCAGCTTCAAATGTGT+TGG 0.425676 8.2:+24537867 None:intergenic
GATTTCATGGCTGAAATTAG+AGG 0.438990 8.2:-24538811 MS.gene60172:CDS
CTATAGTCTTTCCAAAGACT+TGG 0.439881 8.2:-24538175 MS.gene60172:CDS
AGAGATCTTAAGTGGAGAAC+TGG 0.441917 8.2:-24538546 MS.gene60172:CDS
TGCTTGTTGCAATGCTTGTT+CGG 0.447826 8.2:+24538473 None:intergenic
GCTAAGAATCCAGATGCATC+TGG 0.449521 8.2:-24538922 MS.gene60172:CDS
TTGACATTGTGGTGCCTAGC+GGG 0.450378 8.2:+24538388 None:intergenic
GATTTGATAGAGATCTTAAG+TGG 0.457172 8.2:-24538554 MS.gene60172:CDS
AGCTGCTTGGAAAGCAATGC+AGG 0.457790 8.2:-24537928 MS.gene60172:CDS
TACCTTCTTTCCGCCATGCT+TGG 0.457804 8.2:-24538048 MS.gene60172:CDS
ACCAATGCTTGTTTGAATGC+TGG 0.468828 8.2:+24538835 None:intergenic
GGATTGTGAACATTATCGTA+TGG 0.469336 8.2:+24539122 None:intergenic
GGTTTGCCAGGTTTCCGTGT+TGG 0.478336 8.2:-24538153 MS.gene60172:CDS
CTTTGAAACTAGCAATGAAA+AGG 0.480723 8.2:-24537729 MS.gene60172:CDS
GCCGAAGCTTGACATCTTCG+TGG 0.488316 8.2:+24538092 None:intergenic
GAGAAATTTACTCACTAAGT+GGG 0.491942 8.2:-24537644 MS.gene60172:CDS
CCTAAAGGAATTATTCAAAT+GGG 0.495148 8.2:-24539101 MS.gene60172:CDS
CTAAGAAGTATGAAGAGTCT+TGG 0.495354 8.2:+24539168 None:intergenic
GCTTTCGTTGTTGAGTCCAC+TGG 0.495466 8.2:-24537700 MS.gene60172:CDS
ACTCTTCATACTTCTTAGGA+TGG 0.496264 8.2:-24539163 MS.gene60172:CDS
AGAGAAATTTACTCACTAAG+TGG 0.501044 8.2:-24537645 MS.gene60172:CDS
CACCAAGCATGGCGGAAAGA+AGG 0.506326 8.2:+24538046 None:intergenic
CCTTAAAGTCAAAGGAGTAA+TGG 0.507013 8.2:-24538427 MS.gene60172:CDS
GATAATGTTCACAATCCTAA+AGG 0.507661 8.2:-24539116 MS.gene60172:CDS
TCCAAATTTCATCAGCGTCA+TGG 0.509292 8.2:-24538271 MS.gene60172:CDS
GATTGTGAACATTATCGTAT+GGG 0.512439 8.2:+24539123 None:intergenic
CTGGCATGCCATGGTAGTCT+TGG 0.514145 8.2:+24538854 None:intergenic
TGGTGCCTAGCGGGTTTGAT+GGG 0.518811 8.2:+24538397 None:intergenic
TAATTCACTCCTTGACATTG+TGG 0.520906 8.2:+24538377 None:intergenic
GCATCTGGATTCAAAAGAGA+TGG 0.523681 8.2:-24538907 MS.gene60172:CDS
TATAGTCTTTCCAAAGACTT+GGG 0.530946 8.2:-24538174 MS.gene60172:CDS
CTCTTCATACTTCTTAGGAT+GGG 0.532693 8.2:-24539162 MS.gene60172:CDS
TTCCTACTCCTTATTATCCA+GGG 0.533548 8.2:-24538681 MS.gene60172:intron
GCCAGCATTCAAACAAGCAT+TGG 0.538139 8.2:-24538836 MS.gene60172:CDS
GCCACGAAGATGTCAAGCTT+CGG 0.543189 8.2:-24538093 MS.gene60172:CDS
ATGGCGCCAACACGGAAACC+TGG 0.544960 8.2:+24538147 None:intergenic
CGGAGTAGATGGCGCCAACA+CGG 0.550123 8.2:+24538139 None:intergenic
CTTGGAAGAGAGCAAGTTCA+CGG 0.552418 8.2:+24538872 None:intergenic
GTTTGAATGCTGGCATGCCA+TGG 0.557856 8.2:+24538845 None:intergenic
TCTTGTCATTGTACAGAACC+AGG 0.559141 8.2:-24537790 MS.gene60172:CDS
AAAAGCCGGAATTAGCTGCT+TGG 0.561044 8.2:-24537941 MS.gene60172:CDS
GCGGTGAGAACTATGTGGTT+GGG 0.564851 8.2:+24538769 None:intergenic
GTGAAATGTTTAAACCAACT+TGG 0.565975 8.2:+24537821 None:intergenic
AAGAAAATAGTGTACCAAGT+TGG 0.566563 8.2:-24537835 MS.gene60172:CDS
AAAGCAACTTGTAATAGTCA+TGG 0.582182 8.2:-24539191 MS.gene60172:CDS
AAACGCAACCTTAAAGTCAA+AGG 0.585930 8.2:-24538435 MS.gene60172:CDS
TGCGGTGAGAACTATGTGGT+TGG 0.600293 8.2:+24538768 None:intergenic
AAACACACACGAAACCAACC+TGG 0.602737 8.2:+24537772 None:intergenic
CATCTACTCCGAAAATGATA+CGG 0.603585 8.2:-24538127 MS.gene60172:CDS
GTCATTGTACAGAACCAGGT+TGG 0.607425 8.2:-24537786 MS.gene60172:CDS
AACCACATAGTTCTCACCGC+AGG 0.611056 8.2:-24538766 MS.gene60172:CDS
CTGGATAATAAGGAGTAGGA+AGG 0.613137 8.2:+24538683 None:intergenic
GATAATAAGGAGTAGGAAGG+AGG 0.616132 8.2:+24538686 None:intergenic
ATAAGCGACGAGATTTACTC+CGG 0.625337 8.2:-24538309 MS.gene60172:CDS
TGATCAACGTGATCAACAAG+AGG 0.629251 8.2:-24537602 MS.gene60172:CDS
GTTGGTTTAAACATTTCACC+TGG 0.630672 8.2:-24537817 MS.gene60172:CDS
AACGTGATCAACAAGAGGAA+CGG 0.630998 8.2:-24537597 MS.gene60172:CDS
TACCCTGGATAATAAGGAGT+AGG 0.632955 8.2:+24538679 None:intergenic
CGCTAGGCACCACAATGTCA+AGG 0.634181 8.2:-24538386 MS.gene60172:CDS
AACACCATTAACATTGCCAG+TGG 0.635347 8.2:+24537684 None:intergenic
CTTGACATTGTGGTGCCTAG+CGG 0.638500 8.2:+24538387 None:intergenic
GACGCTGATGAAATTTGGAG+AGG 0.641265 8.2:+24538275 None:intergenic
ACAAACCCATCAAACCCGCT+AGG 0.648673 8.2:-24538402 MS.gene60172:CDS
GTGTTTCGCAAACATGTCAG+AGG 0.667703 8.2:-24537755 MS.gene60172:CDS
GCTCTCTTCCAAGACTACCA+TGG 0.688190 8.2:-24538862 MS.gene60172:CDS
ATTTGCTGAAGTAGAACCTG+CGG 0.695572 8.2:+24538750 None:intergenic
GTACAATGACAAGATGAACC+AGG 0.699733 8.2:+24537799 None:intergenic
ATTGCAACAAGCATACCAAG+AGG 0.713636 8.2:-24538463 MS.gene60172:CDS
AACCTGCGGTGAGAACTATG+TGG 0.788440 8.2:+24538764 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTACAAATAAATTTTTA+AGG + chr8.2:24537801-24537820 None:intergenic 10.0%
!! GCAAAAGAAAAAAAAAATAT+TGG + chr8.2:24537829-24537848 None:intergenic 15.0%
! CCTAAAGGAATTATTCAAAT+GGG - chr8.2:24537702-24537721 MS.gene60172:CDS 25.0%
! TCCTAAAGGAATTATTCAAA+TGG - chr8.2:24537701-24537720 MS.gene60172:CDS 25.0%
AAAGCAACTTGTAATAGTCA+TGG - chr8.2:24537612-24537631 MS.gene60172:CDS 30.0%
AAGAAAATAGTGTACCAAGT+TGG - chr8.2:24538968-24538987 MS.gene60172:intron 30.0%
ACAAATATTATCAGCGTCTT+TGG + chr8.2:24538591-24538610 None:intergenic 30.0%
AGAGAAATTTACTCACTAAG+TGG - chr8.2:24539158-24539177 MS.gene60172:CDS 30.0%
CCCATTTGAATAATTCCTTT+AGG + chr8.2:24537705-24537724 None:intergenic 30.0%
GAGAAATTTACTCACTAAGT+GGG - chr8.2:24539159-24539178 MS.gene60172:CDS 30.0%
GATAATGTTCACAATCCTAA+AGG - chr8.2:24537687-24537706 MS.gene60172:CDS 30.0%
GATTGTGAACATTATCGTAT+GGG + chr8.2:24537683-24537702 None:intergenic 30.0%
GATTTGATAGAGATCTTAAG+TGG - chr8.2:24538249-24538268 MS.gene60172:CDS 30.0%
GTGAAATGTTTAAACCAACT+TGG + chr8.2:24538985-24539004 None:intergenic 30.0%
TATAGTCTTTCCAAAGACTT+GGG - chr8.2:24538629-24538648 MS.gene60172:intron 30.0%
! AAAAACAATGTTACTTACCC+TGG + chr8.2:24538142-24538161 None:intergenic 30.0%
! GTTTTCAGCTAAGTAGTTTT+TGG + chr8.2:24538795-24538814 None:intergenic 30.0%
!! CAATGCAGGATTATTTTGTT+GGG - chr8.2:24538889-24538908 MS.gene60172:CDS 30.0%
!! CTTTGAAACTAGCAATGAAA+AGG - chr8.2:24539074-24539093 MS.gene60172:intron 30.0%
!!! TATTTTGTTGGGTTGATATG+AGG - chr8.2:24538900-24538919 MS.gene60172:CDS 30.0%
AAACGCAACCTTAAAGTCAA+AGG - chr8.2:24538368-24538387 MS.gene60172:CDS 35.0%
ACTCTTCATACTTCTTAGGA+TGG - chr8.2:24537640-24537659 MS.gene60172:CDS 35.0%
CAAGACTCTTCATACTTCTT+AGG - chr8.2:24537636-24537655 MS.gene60172:CDS 35.0%
CATCTACTCCGAAAATGATA+CGG - chr8.2:24538676-24538695 MS.gene60172:intron 35.0%
CATTTCAGCTTCAAATGTGT+TGG + chr8.2:24538939-24538958 None:intergenic 35.0%
CCTTAAAGTCAAAGGAGTAA+TGG - chr8.2:24538376-24538395 MS.gene60172:CDS 35.0%
CTATAGTCTTTCCAAAGACT+TGG - chr8.2:24538628-24538647 MS.gene60172:intron 35.0%
CTCTTCATACTTCTTAGGAT+GGG - chr8.2:24537641-24537660 MS.gene60172:CDS 35.0%
GATTTCATGGCTGAAATTAG+AGG - chr8.2:24537992-24538011 MS.gene60172:CDS 35.0%
GGATTGTGAACATTATCGTA+TGG + chr8.2:24537684-24537703 None:intergenic 35.0%
GTTACTTACCCTGGATAATA+AGG + chr8.2:24538133-24538152 None:intergenic 35.0%
TAATTCACTCCTTGACATTG+TGG + chr8.2:24538429-24538448 None:intergenic 35.0%
TTCCTACTCCTTATTATCCA+GGG - chr8.2:24538122-24538141 MS.gene60172:CDS 35.0%
TTCGGTTACTTGAAAGTTGT+TGG + chr8.2:24538315-24538334 None:intergenic 35.0%
TTGAAGCTGAAATGGAACTA+TGG - chr8.2:24538945-24538964 MS.gene60172:CDS 35.0%
! AGCAACAACCGTATCATTTT+CGG + chr8.2:24538687-24538706 None:intergenic 35.0%
! CAACACATTTGAAGCTGAAA+TGG - chr8.2:24538937-24538956 MS.gene60172:CDS 35.0%
! CCATTACTCCTTTGACTTTA+AGG + chr8.2:24538379-24538398 None:intergenic 35.0%
! CTAAGAAGTATGAAGAGTCT+TGG + chr8.2:24537638-24537657 None:intergenic 35.0%
! CTTTTGATCTCTTGGAATCA+TGG - chr8.2:24537855-24537874 MS.gene60172:CDS 35.0%
! GTTGGTTTAAACATTTCACC+TGG - chr8.2:24538986-24539005 MS.gene60172:intron 35.0%
!! ACAAGCATTGGTAGATTTCA+TGG - chr8.2:24537979-24537998 MS.gene60172:CDS 35.0%
!! GCAATGCAGGATTATTTTGT+TGG - chr8.2:24538888-24538907 MS.gene60172:CDS 35.0%
!! TACTCACTAAGTGGGTTTTT+CGG - chr8.2:24539167-24539186 MS.gene60172:CDS 35.0%
!!! GAATTTTTTGTCACCAAGCA+TGG + chr8.2:24538771-24538790 None:intergenic 35.0%
AACACCATTAACATTGCCAG+TGG + chr8.2:24539122-24539141 None:intergenic 40.0%
AACGTGATCAACAAGAGGAA+CGG - chr8.2:24539206-24539225 MS.gene60172:CDS 40.0%
ACCAATGCTTGTTTGAATGC+TGG + chr8.2:24537971-24537990 None:intergenic 40.0%
AGAGATCTTAAGTGGAGAAC+TGG - chr8.2:24538257-24538276 MS.gene60172:CDS 40.0%
ATAAGCGACGAGATTTACTC+CGG - chr8.2:24538494-24538513 MS.gene60172:CDS 40.0%
ATTGCAACAAGCATACCAAG+AGG - chr8.2:24538340-24538359 MS.gene60172:CDS 40.0%
CTGGATAATAAGGAGTAGGA+AGG + chr8.2:24538123-24538142 None:intergenic 40.0%
CTTCCTACTCCTTATTATCC+AGG - chr8.2:24538121-24538140 MS.gene60172:CDS 40.0%
GATAATAAGGAGTAGGAAGG+AGG + chr8.2:24538120-24538139 None:intergenic 40.0%
GCATCTGGATTCAAAAGAGA+TGG - chr8.2:24537896-24537915 MS.gene60172:CDS 40.0%
GTACAATGACAAGATGAACC+AGG + chr8.2:24539007-24539026 None:intergenic 40.0%
TACCCTGGATAATAAGGAGT+AGG + chr8.2:24538127-24538146 None:intergenic 40.0%
TCCAAATTTCATCAGCGTCA+TGG - chr8.2:24538532-24538551 MS.gene60172:CDS 40.0%
TCCATGACGCTGATGAAATT+TGG + chr8.2:24538536-24538555 None:intergenic 40.0%
TCTTGTCATTGTACAGAACC+AGG - chr8.2:24539013-24539032 MS.gene60172:intron 40.0%
TGATCAACGTGATCAACAAG+AGG - chr8.2:24539201-24539220 MS.gene60172:CDS 40.0%
TTGGAGCTTGTGCAATGTAT+TGG + chr8.2:24538296-24538315 None:intergenic 40.0%
! ACGTCAGAACAAGCTTGTTT+CGG - chr8.2:24538832-24538851 MS.gene60172:CDS 40.0%
! ATGTTTTGTCTTGCCGAGAA+AGG - chr8.2:24538085-24538104 MS.gene60172:CDS 40.0%
! ATTTGCTGAAGTAGAACCTG+CGG + chr8.2:24538056-24538075 None:intergenic 40.0%
! CGTATCATTTTCGGAGTAGA+TGG + chr8.2:24538678-24538697 None:intergenic 40.0%
! CTTTTGAATCCAGATGCATC+TGG + chr8.2:24537893-24537912 None:intergenic 40.0%
! TGCTTGTTGCAATGCTTGTT+CGG + chr8.2:24538333-24538352 None:intergenic 40.0%
!! GTTTCGGGTTTACAAAAAGC+CGG - chr8.2:24538848-24538867 MS.gene60172:CDS 40.0%
!!! TTTTTTGTCACCAAGCATGG+CGG + chr8.2:24538768-24538787 None:intergenic 40.0%
AAAAGCCGGAATTAGCTGCT+TGG - chr8.2:24538862-24538881 MS.gene60172:CDS 45.0%
AAACACACACGAAACCAACC+TGG + chr8.2:24539034-24539053 None:intergenic 45.0%
CTTGGAAGAGAGCAAGTTCA+CGG + chr8.2:24537934-24537953 None:intergenic 45.0%
GACGCTGATGAAATTTGGAG+AGG + chr8.2:24538531-24538550 None:intergenic 45.0%
GAGAGCAAGTTCACGGAATA+TGG + chr8.2:24537927-24537946 None:intergenic 45.0%
GAGGAATGCTTCTCCTTTCT+CGG + chr8.2:24538101-24538120 None:intergenic 45.0%
GAGTCCACTGGCAATGTTAA+TGG - chr8.2:24539115-24539134 MS.gene60172:CDS 45.0%
GCCAGCATTCAAACAAGCAT+TGG - chr8.2:24537967-24537986 MS.gene60172:CDS 45.0%
GCTAAGAATCCAGATGCATC+TGG - chr8.2:24537881-24537900 MS.gene60172:CDS 45.0%
GCTTTCCAAGCAGCTAATTC+CGG + chr8.2:24538870-24538889 None:intergenic 45.0%
GTCATTGTACAGAACCAGGT+TGG - chr8.2:24539017-24539036 MS.gene60172:intron 45.0%
GTGTTTCGCAAACATGTCAG+AGG - chr8.2:24539048-24539067 MS.gene60172:intron 45.0%
TGGAGAGGAAAAAACAGTGC+CGG + chr8.2:24538516-24538535 None:intergenic 45.0%
! CGTCAGAACAAGCTTGTTTC+GGG - chr8.2:24538833-24538852 MS.gene60172:CDS 45.0%
! GCAGCTCTCTTTTGATCTCT+TGG - chr8.2:24537847-24537866 MS.gene60172:CDS 45.0%
AACCACATAGTTCTCACCGC+AGG - chr8.2:24538037-24538056 MS.gene60172:CDS 50.0%
AACCTGCGGTGAGAACTATG+TGG + chr8.2:24538042-24538061 None:intergenic 50.0%
ACAAACCCATCAAACCCGCT+AGG - chr8.2:24538401-24538420 MS.gene60172:CDS 50.0%
ACCTGGCAAACCCAAGTCTT+TGG + chr8.2:24538642-24538661 None:intergenic 50.0%
AGCTGCTTGGAAAGCAATGC+AGG - chr8.2:24538875-24538894 MS.gene60172:CDS 50.0%
CTTGACATTGTGGTGCCTAG+CGG + chr8.2:24538419-24538438 None:intergenic 50.0%
GCCACGAAGATGTCAAGCTT+CGG - chr8.2:24538710-24538729 MS.gene60172:CDS 50.0%
GCGGTGAGAACTATGTGGTT+GGG + chr8.2:24538037-24538056 None:intergenic 50.0%
GCTCTCTTCCAAGACTACCA+TGG - chr8.2:24537941-24537960 MS.gene60172:CDS 50.0%
GCTTTCGTTGTTGAGTCCAC+TGG - chr8.2:24539103-24539122 MS.gene60172:CDS 50.0%
GTTTGAATGCTGGCATGCCA+TGG + chr8.2:24537961-24537980 None:intergenic 50.0%
TACCTTCTTTCCGCCATGCT+TGG - chr8.2:24538755-24538774 MS.gene60172:CDS 50.0%
TCCAAAGACTTGGGTTTGCC+AGG - chr8.2:24538638-24538657 MS.gene60172:intron 50.0%
TGCGGTGAGAACTATGTGGT+TGG + chr8.2:24538038-24538057 None:intergenic 50.0%
TTGACATTGTGGTGCCTAGC+GGG + chr8.2:24538418-24538437 None:intergenic 50.0%
! GGTTGCGTTTTTGTGCCTCT+TGG + chr8.2:24538358-24538377 None:intergenic 50.0%
CGCTAGGCACCACAATGTCA+AGG - chr8.2:24538417-24538436 MS.gene60172:CDS 55.0%
CTGGCATGCCATGGTAGTCT+TGG + chr8.2:24537952-24537971 None:intergenic 55.0%
GCCGAAGCTTGACATCTTCG+TGG + chr8.2:24538714-24538733 None:intergenic 55.0%
GGTTTGCCAGGTTTCCGTGT+TGG - chr8.2:24538650-24538669 MS.gene60172:intron 55.0%
! CACCAAGCATGGCGGAAAGA+AGG + chr8.2:24538760-24538779 None:intergenic 55.0%
!! TGGTGCCTAGCGGGTTTGAT+GGG + chr8.2:24538409-24538428 None:intergenic 55.0%
ATGGCGCCAACACGGAAACC+TGG + chr8.2:24538659-24538678 None:intergenic 60.0%
CGGAGTAGATGGCGCCAACA+CGG + chr8.2:24538667-24538686 None:intergenic 60.0%
!! GTGGTGCCTAGCGGGTTTGA+TGG + chr8.2:24538410-24538429 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 24537594 24539231 24537594 ID=MS.gene60172
chr8.2 mRNA 24537594 24539231 24537594 ID=MS.gene60172.t1;Parent=MS.gene60172
chr8.2 exon 24539085 24539231 24539085 ID=MS.gene60172.t1.exon1;Parent=MS.gene60172.t1
chr8.2 CDS 24539085 24539231 24539085 ID=cds.MS.gene60172.t1;Parent=MS.gene60172.t1
chr8.2 exon 24538682 24538974 24538682 ID=MS.gene60172.t1.exon2;Parent=MS.gene60172.t1
chr8.2 CDS 24538682 24538974 24538682 ID=cds.MS.gene60172.t1;Parent=MS.gene60172.t1
chr8.2 exon 24537594 24538575 24537594 ID=MS.gene60172.t1.exon3;Parent=MS.gene60172.t1
chr8.2 CDS 24537594 24538575 24537594 ID=cds.MS.gene60172.t1;Parent=MS.gene60172.t1
Gene Sequence

>MS.gene60172

ATGAAGCTCTTGTCTACAAAAGCAACTTGTAATAGTCATGGCCAAGACTCTTCATACTTCTTAGGATGGGAAGAATACGAGAAAAACCCATACGATAATGTTCACAATCCTAAAGGAATTATTCAAATGGGTCTAGCAGAGAATCAAGTAATTAACTAGTCTTAAACTCTTAATTAATTTTTTTCATAACAACAACAAGAACAACCTTAAAAATTTATTTGTACTTAGCTTACCAATATTTTTTTTTTCTTTTGCAGCTCTCTTTTGATCTCTTGGAATCATGGCTAGCTAAGAATCCAGATGCATCTGGATTCAAAAGAGATGGAAAATCCATATTCCGTGAACTTGCTCTCTTCCAAGACTACCATGGCATGCCAGCATTCAAACAAGCATTGGTAGATTTCATGGCTGAAATTAGAGGAAACAAAGTTACTTTTGATCCCAACCACATAGTTCTCACCGCAGGTTCTACTTCAGCAAATGAGACTCTTATGTTTTGTCTTGCCGAGAAAGGAGAAGCATTCCTCCTTCCTACTCCTTATTATCCAGGGTAAGTAACATTGTTTTTTTTTTATTACTTAAAAACGAATGAAAGCGACAAGAGCTGTAACATCATTTGTGACATAGTTGTTGATATTTTTTTTTTATTTGTGCAGATTTGATAGAGATCTTAAGTGGAGAACTGGTGTTGAGATTGTTCCAATACATTGCACAAGCTCCAACAACTTTCAAGTAACCGAACAAGCATTGCAACAAGCATACCAAGAGGCACAAAAACGCAACCTTAAAGTCAAAGGAGTAATGGTTACAAACCCATCAAACCCGCTAGGCACCACAATGTCAAGGAGTGAATTAAATCTTCTCGTTGACTTTATCAAAGAAAACAAAGACATGCATTTGATAAGCGACGAGATTTACTCCGGCACTGTTTTTTCCTCTCCAAATTTCATCAGCGTCATGGAAATTCTTAAAGACAGAAATGACTTTAACGACTCCAAAGACGCTGATAATATTTGTGAGAGAGTTCATGTTGTCTATAGTCTTTCCAAAGACTTGGGTTTGCCAGGTTTCCGTGTTGGCGCCATCTACTCCGAAAATGATACGGTTGTTGCTGCTGCCACGAAGATGTCAAGCTTCGGCTTAATCTCGTCGCAAACACAATACCTTCTTTCCGCCATGCTTGGTGACAAAAAATTCACCAAAAACTACTTAGCTGAAAACTTAAAGAGACTTAAGAAACGTCAGAACAAGCTTGTTTCGGGTTTACAAAAAGCCGGAATTAGCTGCTTGGAAAGCAATGCAGGATTATTTTGTTGGGTTGATATGAGGCATCTTCTTCATTCCAACACATTTGAAGCTGAAATGGAACTATGGAAGAAAATAGTGTACCAAGTTGGTTTAAACATTTCACCTGGTTCATCTTGTCATTGTACAGAACCAGGTTGGTTTCGTGTGTGTTTCGCAAACATGTCAGAGGAGACTTTGAAACTAGCAATGAAAAGGTTGAAAGCTTTCGTTGTTGAGTCCACTGGCAATGTTAATGGTGTTACAACAAAAAGCACAAAGAGAAATTTACTCACTAAGTGGGTTTTTCGGTTATCGTCTCGTGATCAACGTGATCAACAAGAGGAACGGTAA
Protein sequence

>MS.gene60172.t1

MKLLSTKATCNSHGQDSSYFLGWEEYEKNPYDNVHNPKGIIQMGLAENQLSFDLLESWLAKNPDASGFKRDGKSIFRELALFQDYHGMPAFKQALVDFMAEIRGNKVTFDPNHIVLTAGSTSANETLMFCLAEKGEAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNNFQVTEQALQQAYQEAQKRNLKVKGVMVTNPSNPLGTTMSRSELNLLVDFIKENKDMHLISDEIYSGTVFSSPNFISVMEILKDRNDFNDSKDADNICERVHVVYSLSKDLGLPGFRVGAIYSENDTVVAAATKMSSFGLISSQTQYLLSAMLGDKKFTKNYLAENLKRLKKRQNKLVSGLQKAGISCLESNAGLFCWVDMRHLLHSNTFEAEMELWKKIVYQVGLNISPGSSCHCTEPGWFRVCFANMSEETLKLAMKRLKAFVVESTGNVNGVTTKSTKRNLLTKWVFRLSSRDQRDQQEER