AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06176

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06176.t1 MTR_8g101750 94.943 435 22 0 1 435 1 435 0.0 868
MS.gene06176.t1 MTR_8g101820 88.736 435 36 2 1 435 1 422 0.0 800
MS.gene06176.t1 MTR_6g091760 81.797 434 71 2 1 426 1 434 0.0 756
MS.gene06176.t1 MTR_5g015020 58.621 435 163 6 12 430 4 437 0.0 540
MS.gene06176.t1 MTR_4g097540 57.870 432 164 5 12 426 4 434 0.0 535
MS.gene06176.t1 MTR_3g103550 57.647 425 169 3 12 425 2 426 0.0 531
MS.gene06176.t1 MTR_8g098930 57.277 426 173 2 12 428 12 437 0.0 527
MS.gene06176.t1 MTR_8g028600 55.012 429 185 2 12 433 11 438 0.0 520
MS.gene06176.t1 MTR_7g079080 55.684 431 184 1 12 435 11 441 0.0 519
MS.gene06176.t1 MTR_6g463260 45.047 424 224 2 8 423 100 522 1.64e-144 422
MS.gene06176.t1 MTR_7g079080 55.655 336 149 0 100 435 1 336 5.65e-142 410
MS.gene06176.t1 MTR_5g011400 43.735 407 219 3 24 422 84 488 3.00e-130 385
MS.gene06176.t1 MTR_2g090730 55.405 74 29 1 77 150 31 100 1.13e-20 90.1
MS.gene06176.t1 MTR_8g091280 23.350 394 259 15 46 423 97 463 1.82e-16 81.6
MS.gene06176.t1 MTR_5g033230 24.611 321 219 13 72 372 90 407 2.28e-16 81.3
MS.gene06176.t1 MTR_5g033230 24.595 309 212 12 83 373 103 408 2.69e-16 81.3
MS.gene06176.t1 MTR_4g011820 22.981 322 204 12 115 423 92 382 1.67e-15 78.2
MS.gene06176.t1 MTR_2g090615 23.743 358 230 12 88 429 86 416 3.12e-14 74.7
MS.gene06176.t1 MTR_8g023140 21.925 374 264 14 78 428 149 517 6.37e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06176.t1 AT4G26200 75.524 429 96 3 10 429 16 444 0.0 664
MS.gene06176.t1 AT4G37770 60.000 425 160 2 12 426 4 428 0.0 550
MS.gene06176.t1 AT4G08040 58.531 422 168 1 12 426 2 423 0.0 544
MS.gene06176.t1 AT3G49700 57.763 438 169 4 11 433 3 439 0.0 532
MS.gene06176.t1 AT5G65800 57.981 426 169 3 11 426 3 428 0.0 532
MS.gene06176.t1 AT2G22810 57.176 425 171 3 10 423 2 426 0.0 524
MS.gene06176.t1 AT3G61510 56.161 422 175 2 12 426 11 429 0.0 523
MS.gene06176.t1 AT4G11280 56.019 432 183 1 4 428 7 438 0.0 520
MS.gene06176.t1 AT1G01480 51.598 438 202 3 1 428 1 438 3.35e-172 492
MS.gene06176.t1 AT5G51690 44.313 422 226 3 10 423 67 487 2.20e-138 406
MS.gene06176.t1 AT1G01480 52.711 332 154 2 100 428 1 332 2.28e-134 392
MS.gene06176.t1 AT1G62960 42.402 408 226 5 22 423 143 547 4.71e-127 379
MS.gene06176.t1 AT5G51690 47.230 343 180 1 81 423 23 364 1.15e-123 364
MS.gene06176.t1 AT4G23600 26.197 355 225 13 88 429 78 408 1.81e-18 87.4
MS.gene06176.t1 AT1G23310 25.632 277 188 9 83 344 103 376 6.95e-18 85.9
MS.gene06176.t1 AT1G23310 25.632 277 188 9 83 344 103 376 1.06e-17 85.5
MS.gene06176.t1 AT5G36160 23.757 362 235 13 83 429 80 415 2.89e-17 84.0
MS.gene06176.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.35e-17 83.6
MS.gene06176.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.35e-17 83.6
MS.gene06176.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.35e-17 83.6
MS.gene06176.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.35e-17 83.6
MS.gene06176.t1 AT1G72330 22.606 376 263 14 73 425 160 530 9.76e-17 82.8
MS.gene06176.t1 AT1G72330 23.596 267 190 8 73 328 160 423 3.72e-16 80.5
MS.gene06176.t1 AT1G80360 23.797 395 248 15 41 423 28 381 5.23e-16 79.7
MS.gene06176.t1 AT1G80360 23.797 395 248 15 41 423 28 381 5.23e-16 79.7
MS.gene06176.t1 AT1G80360 23.797 395 248 15 41 423 28 381 5.23e-16 79.7
MS.gene06176.t1 AT1G80360 23.797 395 248 15 41 423 28 381 5.23e-16 79.7
MS.gene06176.t1 AT2G20610 24.513 359 226 14 88 429 109 439 9.97e-16 79.3
MS.gene06176.t1 AT1G77670 24.207 347 238 11 87 430 116 440 1.04e-15 79.3
MS.gene06176.t1 AT4G23600 26.074 326 206 12 117 429 1 304 2.43e-15 77.0
MS.gene06176.t1 AT1G72330 21.851 389 263 14 73 425 160 543 4.47e-15 77.8
MS.gene06176.t1 AT1G17290 22.148 298 212 11 78 359 168 461 1.97e-14 75.9
MS.gene06176.t1 AT4G23600 25.472 318 203 11 88 392 78 374 3.08e-14 74.3
MS.gene06176.t1 AT2G20610 25.461 271 167 9 88 345 109 357 4.11e-14 74.3
MS.gene06176.t1 AT4G23590 24.148 352 236 12 88 429 78 408 5.81e-12 67.8
MS.gene06176.t1 AT5G53970 23.691 363 232 14 88 433 76 410 6.26e-12 67.4
MS.gene06176.t1 AT4G28410 26.619 278 163 11 88 350 111 362 1.04e-11 67.0
MS.gene06176.t1 AT4G28410 25.208 361 229 15 88 433 111 445 1.40e-11 66.6

Find 86 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATATATTAAGATTAAT+AGG 0.143027 4.2:+3325624 MS.gene06176:CDS
GTGATCAACCTGGTTGGTTT+AGG 0.197998 4.2:+3325852 MS.gene06176:CDS
TGTAAGCATAGCAGAGATTC+TGG 0.267334 4.2:+3325394 MS.gene06176:CDS
TGATCAACCTGGTTGGTTTA+GGG 0.271865 4.2:+3325853 MS.gene06176:CDS
GCGGTGATGACTATTCTTTC+CGG 0.294737 4.2:-3324548 None:intergenic
ATAATGACAAAGAAGATATA+AGG 0.344052 4.2:+3325950 MS.gene06176:CDS
ACAAACCCTTCAAACCCTTT+AGG 0.351926 4.2:+3325257 MS.gene06176:CDS
GTCAACTAAGGAAGGTGAAT+TGG 0.354677 4.2:+3325766 MS.gene06176:CDS
TTGAAACCATTATAATCCTC+TGG 0.362383 4.2:-3324425 None:intergenic
GATGAGTTAACTAACTCTTC+AGG 0.373544 4.2:+3324220 MS.gene06176:CDS
GAAAACTCACCCTACTTTGC+TGG 0.379915 4.2:+3324169 MS.gene06176:CDS
AAGTGCAATGGCAAGCTTCA+TGG 0.383493 4.2:+3324493 MS.gene06176:CDS
TATAGTCTCTCTAAAGATCT+TGG 0.393148 4.2:+3325458 MS.gene06176:CDS
GAAATATATTAAGATTAATA+GGG 0.399795 4.2:+3325625 MS.gene06176:CDS
AGGAACAAGCAAGGCATCGC+CGG 0.403862 4.2:-3324618 None:intergenic
GAGTTAGTTAACTCATCATA+AGG 0.406566 4.2:-3324214 None:intergenic
GAGCACCCAGAGGATTATAA+TGG 0.417389 4.2:+3324419 MS.gene06176:CDS
TTTGACCTGTTGCAGTGAAT+TGG 0.417511 4.2:-3325149 None:intergenic
GTTCCTACCCCGTACTATCC+AGG 0.422363 4.2:+3324635 MS.gene06176:CDS
GGAAAGAATACGTAGCTTCA+TGG 0.427457 4.2:+3325919 MS.gene06176:CDS
CGTTGAACTGTTATACCTAA+AGG 0.428028 4.2:-3325272 None:intergenic
GTTGAACTGTTATACCTAAA+GGG 0.430255 4.2:-3325271 None:intergenic
GTTATACCTAAAGGGTTTGA+AGG 0.431593 4.2:-3325263 None:intergenic
AATTTATTCATACAATGATA+AGG 0.432216 4.2:+3325505 MS.gene06176:CDS
CGACTTGGTAGAAAAGTACT+TGG 0.434235 4.2:+3324394 MS.gene06176:CDS
GATTCTTACCCTGGATAGTA+CGG 0.435827 4.2:-3324644 None:intergenic
AGATATGAAATGATTATTGA+AGG 0.438836 4.2:+3325662 MS.gene06176:CDS
TAGTGCCAATTCACTGCAAC+AGG 0.441325 4.2:+3325144 MS.gene06176:CDS
CTGAAATTGAATATATCTCC+AGG 0.445529 4.2:+3325815 MS.gene06176:CDS
GTAGGTTTGATAGAGACTTA+AGG 0.446910 4.2:+3325102 MS.gene06176:intron
GGAACAAGCAAGGCATCGCC+GGG 0.449445 4.2:-3324617 None:intergenic
GCATTATTTCAAGACTATCA+TGG 0.449699 4.2:+3324455 MS.gene06176:CDS
ATTGAAGGCTTGAAAAGTGT+TGG 0.452654 4.2:+3325677 MS.gene06176:CDS
TTAGTTGACTCCTTCAAAAG+TGG 0.458012 4.2:-3325752 None:intergenic
GAGTGTTTGAAAGGGAATGC+AGG 0.465675 4.2:+3325704 MS.gene06176:CDS
AGGAAGGTGAATTGGAACTT+TGG 0.476087 4.2:+3325774 MS.gene06176:CDS
AGTTAGTTAACTCATCATAA+GGG 0.478302 4.2:-3324213 None:intergenic
ACAAACTTTGGATGTTGCAT+TGG 0.480931 4.2:+3325898 MS.gene06176:CDS
GGTCTACCAGGTTTCAGAGT+TGG 0.483669 4.2:+3325479 MS.gene06176:CDS
AAATATGAGTGAACAAACTT+TGG 0.486245 4.2:+3325886 MS.gene06176:CDS
CTCTTCAGGAGTTATACAAA+TGG 0.489531 4.2:+3324234 MS.gene06176:CDS
AGAGACTTAAGGTGGAGAAC+TGG 0.493331 4.2:+3325113 MS.gene06176:CDS
CATCTCAGACGAAATCTACT+CGG 0.501179 4.2:+3325346 MS.gene06176:CDS
GGCTTTCCATCCAGCAAAGT+AGG 0.501359 4.2:-3324179 None:intergenic
ATTCTTACCCTGGATAGTAC+GGG 0.503055 4.2:-3324643 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.510586 4.2:+3325467 MS.gene06176:CDS
TCTTCAGGAGTTATACAAAT+GGG 0.513889 4.2:+3324235 MS.gene06176:CDS
TGGTAGAGCTAAATTTGATC+CGG 0.517167 4.2:+3324529 MS.gene06176:CDS
GCCGGGGTTTGCAAGAATGA+AGG 0.526652 4.2:-3324600 None:intergenic
ACCTTCATTCTTGCAAACCC+CGG 0.529279 4.2:+3324599 MS.gene06176:CDS
ATTGCAATTTCTGACACTCA+TGG 0.531513 4.2:+3324145 MS.gene06176:CDS
GTCATTGTGATCAACCTGGT+TGG 0.538197 4.2:+3325846 MS.gene06176:CDS
TTATACCTAAAGGGTTTGAA+GGG 0.548536 4.2:-3325262 None:intergenic
TGAAACCATTATAATCCTCT+GGG 0.550341 4.2:-3324424 None:intergenic
TAAGCTGCTTCTAAAGCTTG+AGG 0.550801 4.2:-3325188 None:intergenic
AGACGAAATCTACTCGGGCT+CGG 0.552466 4.2:+3325352 MS.gene06176:CDS
TGTGCAGGTTTCGTTCGACT+TGG 0.553387 4.2:+3324379 MS.gene06176:intron
TTGTGTTTGTGAAGATATCA+AGG 0.553420 4.2:-3325561 None:intergenic
GGTTTGATAGAGACTTAAGG+TGG 0.553899 4.2:+3325105 MS.gene06176:intron
TTGAAGGAGTCAACTAAGGA+AGG 0.555608 4.2:+3325758 MS.gene06176:CDS
AGATTTCGTCTGAGATGAGG+TGG 0.558059 4.2:-3325340 None:intergenic
GTTGGAATTGAGTGTTTGAA+AGG 0.569169 4.2:+3325695 MS.gene06176:CDS
ATTAGCCGCAGTTGCTCCTG+CGG 0.571832 4.2:-3324567 None:intergenic
ACTCACCCTACTTTGCTGGA+TGG 0.572647 4.2:+3324173 MS.gene06176:CDS
GGCTAATATGCTATCAGACA+AGG 0.573142 4.2:+3325592 MS.gene06176:CDS
TTGGAATTGAGTGTTTGAAA+GGG 0.574213 4.2:+3325696 MS.gene06176:CDS
GCTTTCCATCCAGCAAAGTA+GGG 0.580141 4.2:-3324178 None:intergenic
AGCTTCATGGAACAAATAAG+AGG 0.582469 4.2:+3324506 MS.gene06176:CDS
TAAATCATTCAGAAGTGCAA+TGG 0.583118 4.2:+3324481 MS.gene06176:CDS
TCTTGTCATTGTGATCAACC+TGG 0.587957 4.2:+3325842 MS.gene06176:CDS
ATCTCAGACGAAATCTACTC+GGG 0.596514 4.2:+3325347 MS.gene06176:CDS
TTCCTACCCCGTACTATCCA+GGG 0.606239 4.2:+3324636 MS.gene06176:CDS
TCAATGAACATGAAAGTGAG+TGG 0.607414 4.2:+3325224 MS.gene06176:CDS
ATTGTTCCAACTCTGAAACC+TGG 0.608322 4.2:-3325485 None:intergenic
GAAAGAATAGTCATCACCGC+AGG 0.624197 4.2:+3324551 MS.gene06176:CDS
AGTAGATTTCGTCTGAGATG+AGG 0.631875 4.2:-3325343 None:intergenic
TGAAATTGAATATATCTCCA+GGG 0.640695 4.2:+3325816 MS.gene06176:CDS
TTCATGGAACAAATAAGAGG+TGG 0.647531 4.2:+3324509 MS.gene06176:CDS
CATCACCGCAGGAGCAACTG+CGG 0.654637 4.2:+3324562 MS.gene06176:CDS
TACCCTGGATAGTACGGGGT+AGG 0.665259 4.2:-3324638 None:intergenic
GTACGGGGTAGGAACAAGCA+AGG 0.685560 4.2:-3324627 None:intergenic
AAACAGACCCTAAACCAACC+AGG 0.685588 4.2:-3325860 None:intergenic
TTCTTACCCTGGATAGTACG+GGG 0.696996 4.2:-3324642 None:intergenic
TCACAATGACAAGAACACCC+TGG 0.704106 4.2:-3325833 None:intergenic
GAACAAGCAAGGCATCGCCG+GGG 0.751656 4.2:-3324616 None:intergenic
GTACTTGGAAGAGCACCCAG+AGG 0.784244 4.2:+3324409 MS.gene06176:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATATATTAAGATTAAT+AGG + chr4.2:3325624-3325643 MS.gene06176:CDS 10.0%
!! GAAATATATTAAGATTAATA+GGG + chr4.2:3325625-3325644 MS.gene06176:CDS 10.0%
!! AATTTATTCATACAATGATA+AGG + chr4.2:3325505-3325524 MS.gene06176:CDS 15.0%
!! AGATATGAAATGATTATTGA+AGG + chr4.2:3325662-3325681 MS.gene06176:CDS 20.0%
! AAATATGAGTGAACAAACTT+TGG + chr4.2:3325886-3325905 MS.gene06176:CDS 25.0%
! AGTTAGTTAACTCATCATAA+GGG - chr4.2:3324216-3324235 None:intergenic 25.0%
! CACTTCATCATAAAGAAAAA+AGG - chr4.2:3324947-3324966 None:intergenic 25.0%
! TATATTAATGTGTATTGTGC+AGG + chr4.2:3324364-3324383 MS.gene06176:intron 25.0%
! TGAAATTGAATATATCTCCA+GGG + chr4.2:3325816-3325835 MS.gene06176:CDS 25.0%
!! TCTTTTTCTTGTACTTTTGT+AGG + chr4.2:3325084-3325103 MS.gene06176:intron 25.0%
!!! ATATTACTTGAGAGTGTTTA+TGG - chr4.2:3324838-3324857 None:intergenic 25.0%
ATAGTTAGTTCCAAAACCAT+TGG - chr4.2:3324345-3324364 None:intergenic 30.0%
CTGAAATTGAATATATCTCC+AGG + chr4.2:3325815-3325834 MS.gene06176:CDS 30.0%
GAGTTAGTTAACTCATCATA+AGG - chr4.2:3324217-3324236 None:intergenic 30.0%
GCATTATTTCAAGACTATCA+TGG + chr4.2:3324455-3324474 MS.gene06176:CDS 30.0%
GTTGAACTGTTATACCTAAA+GGG - chr4.2:3325274-3325293 None:intergenic 30.0%
TCTTCAGGAGTTATACAAAT+GGG + chr4.2:3324235-3324254 MS.gene06176:CDS 30.0%
TGAAACCATTATAATCCTCT+GGG - chr4.2:3324427-3324446 None:intergenic 30.0%
TTATACCTAAAGGGTTTGAA+GGG - chr4.2:3325265-3325284 None:intergenic 30.0%
TTGAAACCATTATAATCCTC+TGG - chr4.2:3324428-3324447 None:intergenic 30.0%
TTGGAATTGAGTGTTTGAAA+GGG + chr4.2:3325696-3325715 MS.gene06176:CDS 30.0%
TTGTGTTTGTGAAGATATCA+AGG - chr4.2:3325564-3325583 None:intergenic 30.0%
! ATCATAAGGGTTTTCATCAT+AGG - chr4.2:3324203-3324222 None:intergenic 30.0%
! TAAATCATTCAGAAGTGCAA+TGG + chr4.2:3324481-3324500 MS.gene06176:CDS 30.0%
! TATAGTCTCTCTAAAGATCT+TGG + chr4.2:3325458-3325477 MS.gene06176:CDS 30.0%
! TTCACAAACACAACAGTTTT+TGG + chr4.2:3325571-3325590 MS.gene06176:CDS 30.0%
!! ACTATTTTTCATAACTCTCG+AGG - chr4.2:3324791-3324810 None:intergenic 30.0%
AAACCATTGGTGAACGAATT+AGG - chr4.2:3324332-3324351 None:intergenic 35.0%
ACAAACTTTGGATGTTGCAT+TGG + chr4.2:3325898-3325917 MS.gene06176:CDS 35.0%
AGCTTCATGGAACAAATAAG+AGG + chr4.2:3324506-3324525 MS.gene06176:CDS 35.0%
ATTGCAATTTCTGACACTCA+TGG + chr4.2:3324145-3324164 MS.gene06176:CDS 35.0%
CGTTGAACTGTTATACCTAA+AGG - chr4.2:3325275-3325294 None:intergenic 35.0%
CTCTTCAGGAGTTATACAAA+TGG + chr4.2:3324234-3324253 MS.gene06176:CDS 35.0%
GAATCAAGCACCATACAATA+CGG + chr4.2:3324662-3324681 MS.gene06176:intron 35.0%
GATGAGTTAACTAACTCTTC+AGG + chr4.2:3324220-3324239 MS.gene06176:CDS 35.0%
GTAGGTTTGATAGAGACTTA+AGG + chr4.2:3325102-3325121 MS.gene06176:intron 35.0%
GTTATACCTAAAGGGTTTGA+AGG - chr4.2:3325266-3325285 None:intergenic 35.0%
GTTGGAATTGAGTGTTTGAA+AGG + chr4.2:3325695-3325714 MS.gene06176:CDS 35.0%
TCAATGAACATGAAAGTGAG+TGG + chr4.2:3325224-3325243 MS.gene06176:CDS 35.0%
TGGTAGAGCTAAATTTGATC+CGG + chr4.2:3324529-3324548 MS.gene06176:CDS 35.0%
TTCATGGAACAAATAAGAGG+TGG + chr4.2:3324509-3324528 MS.gene06176:CDS 35.0%
! AATTCGTTCACCAATGGTTT+TGG + chr4.2:3324332-3324351 MS.gene06176:intron 35.0%
! ATTGAAGGCTTGAAAAGTGT+TGG + chr4.2:3325677-3325696 MS.gene06176:CDS 35.0%
! TTAGTTGACTCCTTCAAAAG+TGG - chr4.2:3325755-3325774 None:intergenic 35.0%
!! ACTTTTGAAGGAGTCAACTA+AGG + chr4.2:3325754-3325773 MS.gene06176:CDS 35.0%
!! GAATTTGAGTCCACTTTTGA+AGG + chr4.2:3325742-3325761 MS.gene06176:CDS 35.0%
AAGCTAGCTAGCTAGCTAAA+AGG - chr4.2:3324983-3325002 None:intergenic 40.0%
ACAAACCCTTCAAACCCTTT+AGG + chr4.2:3325257-3325276 MS.gene06176:CDS 40.0%
ACATATAGTCTACGCAAGCA+TGG - chr4.2:3325045-3325064 None:intergenic 40.0%
AGGAAGGTGAATTGGAACTT+TGG + chr4.2:3325774-3325793 MS.gene06176:CDS 40.0%
ATCTCAGACGAAATCTACTC+GGG + chr4.2:3325347-3325366 MS.gene06176:CDS 40.0%
ATTCTTACCCTGGATAGTAC+GGG - chr4.2:3324646-3324665 None:intergenic 40.0%
CATCTCAGACGAAATCTACT+CGG + chr4.2:3325346-3325365 MS.gene06176:CDS 40.0%
CCAAACATAGCCGTATTGTA+TGG - chr4.2:3324675-3324694 None:intergenic 40.0%
CCATACAATACGGCTATGTT+TGG + chr4.2:3324672-3324691 MS.gene06176:intron 40.0%
GATTCTTACCCTGGATAGTA+CGG - chr4.2:3324647-3324666 None:intergenic 40.0%
GGAAAGAATACGTAGCTTCA+TGG + chr4.2:3325919-3325938 MS.gene06176:CDS 40.0%
GGCTAATATGCTATCAGACA+AGG + chr4.2:3325592-3325611 MS.gene06176:CDS 40.0%
GGTTTGATAGAGACTTAAGG+TGG + chr4.2:3325105-3325124 MS.gene06176:intron 40.0%
GTCAACTAAGGAAGGTGAAT+TGG + chr4.2:3325766-3325785 MS.gene06176:CDS 40.0%
TCTAAAGATCTTGGTCTACC+AGG + chr4.2:3325467-3325486 MS.gene06176:CDS 40.0%
TCTTGTCATTGTGATCAACC+TGG + chr4.2:3325842-3325861 MS.gene06176:CDS 40.0%
TGTAAGCATAGCAGAGATTC+TGG + chr4.2:3325394-3325413 MS.gene06176:CDS 40.0%
TTGAAGGAGTCAACTAAGGA+AGG + chr4.2:3325758-3325777 MS.gene06176:CDS 40.0%
TTTGACCTGTTGCAGTGAAT+TGG - chr4.2:3325152-3325171 None:intergenic 40.0%
! AGTAGATTTCGTCTGAGATG+AGG - chr4.2:3325346-3325365 None:intergenic 40.0%
!! ATTGTTCCAACTCTGAAACC+TGG - chr4.2:3325488-3325507 None:intergenic 40.0%
!! CGACTTGGTAGAAAAGTACT+TGG + chr4.2:3324394-3324413 MS.gene06176:CDS 40.0%
!! GGAATGCAGGATTGTTTTGT+TGG + chr4.2:3325717-3325736 MS.gene06176:CDS 40.0%
!! TAAGCTGCTTCTAAAGCTTG+AGG - chr4.2:3325191-3325210 None:intergenic 40.0%
!! TGATCAACCTGGTTGGTTTA+GGG + chr4.2:3325853-3325872 MS.gene06176:CDS 40.0%
AAACAGACCCTAAACCAACC+AGG - chr4.2:3325863-3325882 None:intergenic 45.0%
AAGTGCAATGGCAAGCTTCA+TGG + chr4.2:3324493-3324512 MS.gene06176:CDS 45.0%
ACCTTCATTCTTGCAAACCC+CGG + chr4.2:3324599-3324618 MS.gene06176:CDS 45.0%
AGAGACTTAAGGTGGAGAAC+TGG + chr4.2:3325113-3325132 MS.gene06176:CDS 45.0%
ATGGTGCTTGATTCTTACCC+TGG - chr4.2:3324656-3324675 None:intergenic 45.0%
GAAAACTCACCCTACTTTGC+TGG + chr4.2:3324169-3324188 MS.gene06176:CDS 45.0%
GAAAGAATAGTCATCACCGC+AGG + chr4.2:3324551-3324570 MS.gene06176:CDS 45.0%
GAGCACCCAGAGGATTATAA+TGG + chr4.2:3324419-3324438 MS.gene06176:CDS 45.0%
GAGTGTTTGAAAGGGAATGC+AGG + chr4.2:3325704-3325723 MS.gene06176:CDS 45.0%
GCGGTGATGACTATTCTTTC+CGG - chr4.2:3324551-3324570 None:intergenic 45.0%
GCTCCTAATTCGTTCACCAA+TGG + chr4.2:3324326-3324345 MS.gene06176:intron 45.0%
GCTTTCCATCCAGCAAAGTA+GGG - chr4.2:3324181-3324200 None:intergenic 45.0%
GTCATTGTGATCAACCTGGT+TGG + chr4.2:3325846-3325865 MS.gene06176:CDS 45.0%
TAGTGCCAATTCACTGCAAC+AGG + chr4.2:3325144-3325163 MS.gene06176:CDS 45.0%
TCACAATGACAAGAACACCC+TGG - chr4.2:3325836-3325855 None:intergenic 45.0%
TTCTTACCCTGGATAGTACG+GGG - chr4.2:3324645-3324664 None:intergenic 45.0%
! AGATTTCGTCTGAGATGAGG+TGG - chr4.2:3325343-3325362 None:intergenic 45.0%
!! GTGATCAACCTGGTTGGTTT+AGG + chr4.2:3325852-3325871 MS.gene06176:CDS 45.0%
ACTCACCCTACTTTGCTGGA+TGG + chr4.2:3324173-3324192 MS.gene06176:CDS 50.0%
AGACGAAATCTACTCGGGCT+CGG + chr4.2:3325352-3325371 MS.gene06176:CDS 50.0%
GGCTTTCCATCCAGCAAAGT+AGG - chr4.2:3324182-3324201 None:intergenic 50.0%
GGTCTACCAGGTTTCAGAGT+TGG + chr4.2:3325479-3325498 MS.gene06176:CDS 50.0%
TGTGCAGGTTTCGTTCGACT+TGG + chr4.2:3324379-3324398 MS.gene06176:intron 50.0%
TTCCTACCCCGTACTATCCA+GGG + chr4.2:3324636-3324655 MS.gene06176:CDS 50.0%
ATTAGCCGCAGTTGCTCCTG+CGG - chr4.2:3324570-3324589 None:intergenic 55.0%
GCCGGGGTTTGCAAGAATGA+AGG - chr4.2:3324603-3324622 None:intergenic 55.0%
GTACGGGGTAGGAACAAGCA+AGG - chr4.2:3324630-3324649 None:intergenic 55.0%
GTTCCTACCCCGTACTATCC+AGG + chr4.2:3324635-3324654 MS.gene06176:CDS 55.0%
TACCCTGGATAGTACGGGGT+AGG - chr4.2:3324641-3324660 None:intergenic 55.0%
!! AGGAACAAGCAAGGCATCGC+CGG - chr4.2:3324621-3324640 None:intergenic 55.0%
!! GTACTTGGAAGAGCACCCAG+AGG + chr4.2:3324409-3324428 MS.gene06176:CDS 55.0%
CATCACCGCAGGAGCAACTG+CGG + chr4.2:3324562-3324581 MS.gene06176:CDS 60.0%
!! GAACAAGCAAGGCATCGCCG+GGG - chr4.2:3324619-3324638 None:intergenic 60.0%
!! GGAACAAGCAAGGCATCGCC+GGG - chr4.2:3324620-3324639 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 3324103 3325970 3324103 ID=MS.gene06176
chr4.2 mRNA 3324103 3325970 3324103 ID=MS.gene06176.t1;Parent=MS.gene06176
chr4.2 exon 3324103 3324273 3324103 ID=MS.gene06176.t1.exon1;Parent=MS.gene06176.t1
chr4.2 CDS 3324103 3324273 3324103 ID=cds.MS.gene06176.t1;Parent=MS.gene06176.t1
chr4.2 exon 3324386 3324657 3324386 ID=MS.gene06176.t1.exon2;Parent=MS.gene06176.t1
chr4.2 CDS 3324386 3324657 3324386 ID=cds.MS.gene06176.t1;Parent=MS.gene06176.t1
chr4.2 exon 3325106 3325970 3325106 ID=MS.gene06176.t1.exon3;Parent=MS.gene06176.t1
chr4.2 CDS 3325106 3325970 3325106 ID=cds.MS.gene06176.t1;Parent=MS.gene06176.t1
Gene Sequence

>MS.gene06176

ATGGGTATTGAGATAGAACAATCTTGTGTACAACTTTCAAACATTGCAATTTCTGACACTCATGGAGAAAACTCACCCTACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATGATGAGTTAACTAACTCTTCAGGAGTTATACAAATGGGATTAGCAGAAAATCAAGTATGTTTATATATCTTGAATTAATAAGTCTTATTATGTCTATATATAATTAGCTCCTAATTCGTTCACCAATGGTTTTGGAACTAACTATATATTAATGTGTATTGTGCAGGTTTCGTTCGACTTGGTAGAAAAGTACTTGGAAGAGCACCCAGAGGATTATAATGGTTTCAAAGAGAATGCATTATTTCAAGACTATCATGGACTTAAATCATTCAGAAGTGCAATGGCAAGCTTCATGGAACAAATAAGAGGTGGTAGAGCTAAATTTGATCCGGAAAGAATAGTCATCACCGCAGGAGCAACTGCGGCTAATGAGCTTTTAACCTTCATTCTTGCAAACCCCGGCGATGCCTTGCTTGTTCCTACCCCGTACTATCCAGGGTAAGAATCAAGCACCATACAATACGGCTATGTTTGGATAAACAATTTAATTGATAAACTATGTCTATAATACATGATAAAATACAACTAAACTATTTTATATAAGTTATAAATTGTTTTCACTAGCATTCCTCGAGAGTTATGAAAAATAGTTTAAGCAGAAGTCATAAGTTGTTTCCATAAACACTCTCAAGTAATATCAGTAGATAAATTCAAATAAATCAATTCAAAAAGAGTGCATATGTCTTAATAACACGCGTAAAATTTCACACATTTATTTCATGCACCTTTTTTCTTTATGATGAAGTGCTATTATTAAAAACCTTTTAGCTAGCTAGCTAGCTTCATTTGCTTTAATTTATCATTAGAAGCTATAGTATGTAACCATGCTTGCGTAGACTATATGTGATGAAATAATCTCATGTATCTTTTTCTTGTACTTTTGTAGGTTTGATAGAGACTTAAGGTGGAGAACTGGTGTAAACATAGTGCCAATTCACTGCAACAGGTCAAACAATTTTCAAATTACACCTCAAGCTTTAGAAGCAGCTTACAAAGAAGCAGAATCAATGAACATGAAAGTGAGTGGAGTACTCATCACAAACCCTTCAAACCCTTTAGGTATAACAGTTCAACGCACAGTTCTTGAAGAGATTCTTGACTTTGTGACTAAAAAGAACATCCACCTCATCTCAGACGAAATCTACTCGGGCTCGGTCTTTAATTCATCTGAGTTTGTAAGCATAGCAGAGATTCTGGAAGCTCGTCGATACAAAGATGCAGAGAGAGTTCACATTGTTTATAGTCTCTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTTGGAACAATTTATTCATACAATGATAAGGTTGTAACAACAGCGCGAAGAATGTCGAGTTTTACCTTGATATCTTCACAAACACAACAGTTTTTGGCTAATATGCTATCAGACAAGGAGTTTACAGAGAAATATATTAAGATTAATAGGGAGAGATTAAAGAAGAGATATGAAATGATTATTGAAGGCTTGAAAAGTGTTGGAATTGAGTGTTTGAAAGGGAATGCAGGATTGTTTTGTTGGATGAATTTGAGTCCACTTTTGAAGGAGTCAACTAAGGAAGGTGAATTGGAACTTTGGAATGTTGTTTTGAATGAACTGAAATTGAATATATCTCCAGGGTGTTCTTGTCATTGTGATCAACCTGGTTGGTTTAGGGTCTGTTTTGCAAATATGAGTGAACAAACTTTGGATGTTGCATTGGAAAGAATACGTAGCTTCATGGCTAGAAGAATAATGACAAAGAAGATATAA
Protein sequence

>MS.gene06176.t1

MGIEIEQSCVQLSNIAISDTHGENSPYFAGWKAYDENPYDELTNSSGVIQMGLAENQVSFDLVEKYLEEHPEDYNGFKENALFQDYHGLKSFRSAMASFMEQIRGGRAKFDPERIVITAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCNRSNNFQITPQALEAAYKEAESMNMKVSGVLITNPSNPLGITVQRTVLEEILDFVTKKNIHLISDEIYSGSVFNSSEFVSIAEILEARRYKDAERVHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQQFLANMLSDKEFTEKYIKINRERLKKRYEMIIEGLKSVGIECLKGNAGLFCWMNLSPLLKESTKEGELELWNVVLNELKLNISPGCSCHCDQPGWFRVCFANMSEQTLDVALERIRSFMARRIMTKKI