AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20160.t1 MTR_8g101750 95.172 435 21 0 1 435 1 435 0.0 869
MS.gene20160.t1 MTR_8g101820 88.966 435 35 2 1 435 1 422 0.0 801
MS.gene20160.t1 MTR_6g091760 82.028 434 70 2 1 426 1 434 0.0 758
MS.gene20160.t1 MTR_5g015020 58.621 435 163 6 12 430 4 437 0.0 539
MS.gene20160.t1 MTR_4g097540 57.870 432 164 5 12 426 4 434 0.0 536
MS.gene20160.t1 MTR_3g103550 57.647 425 169 3 12 425 2 426 0.0 532
MS.gene20160.t1 MTR_8g098930 57.277 426 173 2 12 428 12 437 0.0 528
MS.gene20160.t1 MTR_8g028600 55.245 429 184 2 12 433 11 438 0.0 521
MS.gene20160.t1 MTR_7g079080 55.916 431 183 1 12 435 11 441 0.0 520
MS.gene20160.t1 MTR_6g463260 45.047 424 224 2 8 423 100 522 1.07e-144 423
MS.gene20160.t1 MTR_7g079080 55.952 336 148 0 100 435 1 336 1.92e-142 412
MS.gene20160.t1 MTR_5g011400 43.735 407 219 3 24 422 84 488 3.00e-130 385
MS.gene20160.t1 MTR_2g090730 55.405 74 29 1 77 150 31 100 1.12e-20 90.1
MS.gene20160.t1 MTR_8g091280 23.350 394 259 15 46 423 97 463 1.00e-16 82.4
MS.gene20160.t1 MTR_5g033230 24.611 321 219 13 72 372 90 407 2.58e-16 81.3
MS.gene20160.t1 MTR_5g033230 24.595 309 212 12 83 373 103 408 3.08e-16 80.9
MS.gene20160.t1 MTR_4g011820 22.981 322 204 12 115 423 92 382 1.20e-15 78.6
MS.gene20160.t1 MTR_2g090615 24.022 358 229 12 88 429 86 416 1.15e-14 75.9
MS.gene20160.t1 MTR_8g023140 22.102 371 261 14 78 425 149 514 6.66e-14 73.9
MS.gene20160.t1 MTR_8g061360 22.167 406 266 17 44 429 38 413 8.56e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20160.t1 AT4G26200 75.758 429 95 3 10 429 16 444 0.0 666
MS.gene20160.t1 AT4G37770 60.000 425 160 2 12 426 4 428 0.0 551
MS.gene20160.t1 AT4G08040 58.768 422 167 1 12 426 2 423 0.0 545
MS.gene20160.t1 AT3G49700 57.534 438 170 4 11 433 3 439 0.0 530
MS.gene20160.t1 AT5G65800 57.746 426 170 3 11 426 3 428 0.0 530
MS.gene20160.t1 AT2G22810 57.176 425 171 3 10 423 2 426 0.0 525
MS.gene20160.t1 AT3G61510 56.161 422 175 2 12 426 11 429 0.0 524
MS.gene20160.t1 AT4G11280 56.250 432 182 1 4 428 7 438 0.0 522
MS.gene20160.t1 AT1G01480 51.598 438 202 3 1 428 1 438 1.33e-172 493
MS.gene20160.t1 AT5G51690 44.313 422 226 3 10 423 67 487 1.59e-138 406
MS.gene20160.t1 AT1G01480 52.711 332 154 2 100 428 1 332 7.42e-135 393
MS.gene20160.t1 AT1G62960 42.402 408 226 5 22 423 143 547 3.96e-127 379
MS.gene20160.t1 AT5G51690 47.230 343 180 1 81 423 23 364 9.22e-124 364
MS.gene20160.t1 AT4G23600 26.479 355 224 13 88 429 78 408 5.59e-19 89.0
MS.gene20160.t1 AT1G23310 25.632 277 188 9 83 344 103 376 6.58e-18 85.9
MS.gene20160.t1 AT5G36160 24.033 362 234 13 83 429 80 415 9.18e-18 85.5
MS.gene20160.t1 AT1G23310 25.632 277 188 9 83 344 103 376 1.10e-17 85.5
MS.gene20160.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.45e-17 83.6
MS.gene20160.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.45e-17 83.6
MS.gene20160.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.45e-17 83.6
MS.gene20160.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.45e-17 83.6
MS.gene20160.t1 AT1G72330 22.606 376 263 14 73 425 160 530 9.00e-17 82.8
MS.gene20160.t1 AT2G20610 24.791 359 225 14 88 429 109 439 2.89e-16 81.3
MS.gene20160.t1 AT1G80360 23.797 395 248 15 41 423 28 381 3.62e-16 80.5
MS.gene20160.t1 AT1G80360 23.797 395 248 15 41 423 28 381 3.62e-16 80.5
MS.gene20160.t1 AT1G80360 23.797 395 248 15 41 423 28 381 3.62e-16 80.5
MS.gene20160.t1 AT1G80360 23.797 395 248 15 41 423 28 381 3.62e-16 80.5
MS.gene20160.t1 AT1G72330 23.596 267 190 8 73 328 160 423 3.83e-16 80.5
MS.gene20160.t1 AT1G77670 24.207 347 238 11 87 430 116 440 6.99e-16 79.7
MS.gene20160.t1 AT4G23600 26.380 326 205 12 117 429 1 304 7.04e-16 78.6
MS.gene20160.t1 AT1G72330 21.851 389 263 14 73 425 160 543 4.55e-15 77.8
MS.gene20160.t1 AT1G17290 22.148 298 212 11 78 359 168 461 2.02e-14 75.5
MS.gene20160.t1 AT4G23600 25.472 318 203 11 88 392 78 374 3.11e-14 74.3
MS.gene20160.t1 AT2G20610 25.461 271 167 9 88 345 109 357 4.11e-14 74.3
MS.gene20160.t1 AT4G23590 24.432 352 235 12 88 429 78 408 1.83e-12 69.3
MS.gene20160.t1 AT5G53970 23.967 363 231 14 88 433 76 410 2.23e-12 68.9
MS.gene20160.t1 AT4G28410 25.479 365 223 16 88 433 111 445 4.52e-12 68.2
MS.gene20160.t1 AT4G28410 26.619 278 163 11 88 350 111 362 1.05e-11 67.0

Find 86 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATATATTAAGATTAAT+AGG 0.143027 4.4:+3842295 MS.gene20160:CDS
GTGATCAACCTGGTTGGTTT+AGG 0.197998 4.4:+3842523 MS.gene20160:CDS
TGTAAGCATAGCAGAGATTC+TGG 0.267334 4.4:+3842065 MS.gene20160:CDS
TGATCAACCTGGTTGGTTTA+GGG 0.271865 4.4:+3842524 MS.gene20160:CDS
GCGGTGATGACTATTCTTTC+CGG 0.294737 4.4:-3841219 None:intergenic
ATAATGACAAAGAAGATATA+AGG 0.344052 4.4:+3842621 MS.gene20160:CDS
ACAAACCCTTCAAACCCTTT+AGG 0.351926 4.4:+3841928 MS.gene20160:CDS
GTCAACTAAGGAAGGTGAAT+TGG 0.354677 4.4:+3842437 MS.gene20160:CDS
TTGAAACCATTATAATCCTC+TGG 0.362383 4.4:-3841096 None:intergenic
GATGAGTTAACTAACTCTTC+AGG 0.373544 4.4:+3840891 MS.gene20160:CDS
GAAAACTCACCCTACTTTGC+TGG 0.379915 4.4:+3840840 MS.gene20160:CDS
AAGTGCAATGGCAAGCTTCA+TGG 0.383493 4.4:+3841164 MS.gene20160:CDS
TATAGTCTCTCTAAAGATCT+TGG 0.393148 4.4:+3842129 MS.gene20160:CDS
GAAATATATTAAGATTAATA+GGG 0.399795 4.4:+3842296 MS.gene20160:CDS
AGGAACAAGCAAGGCATCGC+CGG 0.403862 4.4:-3841289 None:intergenic
GAGTTAGTTAACTCATCATA+AGG 0.406566 4.4:-3840885 None:intergenic
GAGCACCCAGAGGATTATAA+TGG 0.417389 4.4:+3841090 MS.gene20160:CDS
TTTGACCTGTTGCAGTGAAT+TGG 0.417511 4.4:-3841820 None:intergenic
GTTCCTACCCCGTACTATCC+AGG 0.422363 4.4:+3841306 MS.gene20160:CDS
GGAAAGAATACGTAGCTTCA+TGG 0.427457 4.4:+3842590 MS.gene20160:CDS
CGTTGAACTGTTATACCTAA+AGG 0.428028 4.4:-3841943 None:intergenic
GTTGAACTGTTATACCTAAA+GGG 0.430255 4.4:-3841942 None:intergenic
GTTATACCTAAAGGGTTTGA+AGG 0.431593 4.4:-3841934 None:intergenic
AATTTATTCATACAATGATA+AGG 0.432216 4.4:+3842176 MS.gene20160:CDS
CGACTTGGTAGAAAAGTACT+TGG 0.434235 4.4:+3841065 MS.gene20160:CDS
GATTCTTACCCTGGATAGTA+CGG 0.435827 4.4:-3841315 None:intergenic
AGATATGAAATGATTATTGA+AGG 0.438836 4.4:+3842333 MS.gene20160:CDS
TAGTGCCAATTCACTGCAAC+AGG 0.441325 4.4:+3841815 MS.gene20160:CDS
CTGAAATTGAATATATCTCC+AGG 0.445529 4.4:+3842486 MS.gene20160:CDS
ACAAACTTTGGAAGTTGCAT+TGG 0.446683 4.4:+3842569 MS.gene20160:CDS
GTAGGTTTGATAGAGACTTA+AGG 0.446910 4.4:+3841773 MS.gene20160:intron
GGAACAAGCAAGGCATCGCC+GGG 0.449445 4.4:-3841288 None:intergenic
GCATTATTTCAAGACTATCA+TGG 0.449699 4.4:+3841126 MS.gene20160:CDS
ATTGAAGGCTTGAAAAGTGT+TGG 0.452654 4.4:+3842348 MS.gene20160:CDS
TTAGTTGACTCCTTCAAAAG+TGG 0.458012 4.4:-3842423 None:intergenic
GAGTGTTTGAAAGGGAATGC+AGG 0.465675 4.4:+3842375 MS.gene20160:CDS
AGGAAGGTGAATTGGAACTT+TGG 0.476087 4.4:+3842445 MS.gene20160:CDS
AGTTAGTTAACTCATCATAA+GGG 0.478302 4.4:-3840884 None:intergenic
AAATATGAGTGAACAAACTT+TGG 0.478309 4.4:+3842557 MS.gene20160:CDS
GGTCTACCAGGTTTCAGAGT+TGG 0.483669 4.4:+3842150 MS.gene20160:CDS
CTCTTCAGGAGTTATACAAA+TGG 0.489531 4.4:+3840905 MS.gene20160:CDS
AGAGACTTAAGGTGGAGAAC+TGG 0.493331 4.4:+3841784 MS.gene20160:CDS
CATCTCAGACGAAATCTACT+CGG 0.501179 4.4:+3842017 MS.gene20160:CDS
GGCTTTCCATCCAGCAAAGT+AGG 0.501359 4.4:-3840850 None:intergenic
ATTCTTACCCTGGATAGTAC+GGG 0.503055 4.4:-3841314 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.510586 4.4:+3842138 MS.gene20160:CDS
TCTTCAGGAGTTATACAAAT+GGG 0.513889 4.4:+3840906 MS.gene20160:CDS
TGGTAGAGCTAAATTTGATC+CGG 0.517167 4.4:+3841200 MS.gene20160:CDS
GCCGGGGTTTGCAAGAATGA+AGG 0.526652 4.4:-3841271 None:intergenic
ACCTTCATTCTTGCAAACCC+CGG 0.529279 4.4:+3841270 MS.gene20160:CDS
ATTGCAATTTCTGACACTCA+TGG 0.531513 4.4:+3840816 MS.gene20160:CDS
GTCATTGTGATCAACCTGGT+TGG 0.538197 4.4:+3842517 MS.gene20160:CDS
TTATACCTAAAGGGTTTGAA+GGG 0.548536 4.4:-3841933 None:intergenic
TGAAACCATTATAATCCTCT+GGG 0.550341 4.4:-3841095 None:intergenic
TAAGCTGCTTCTAAAGCTTG+AGG 0.550801 4.4:-3841859 None:intergenic
AGACGAAATCTACTCGGGCT+CGG 0.552466 4.4:+3842023 MS.gene20160:CDS
TGTGCAGGTTTCGTTCGACT+TGG 0.553387 4.4:+3841050 MS.gene20160:intron
TTGTGTTTGTGAAGATATCA+AGG 0.553420 4.4:-3842232 None:intergenic
GGTTTGATAGAGACTTAAGG+TGG 0.553899 4.4:+3841776 MS.gene20160:intron
TTGAAGGAGTCAACTAAGGA+AGG 0.555608 4.4:+3842429 MS.gene20160:CDS
AGATTTCGTCTGAGATGAGG+TGG 0.558059 4.4:-3842011 None:intergenic
GTTGGAATTGAGTGTTTGAA+AGG 0.569169 4.4:+3842366 MS.gene20160:CDS
ATTAGCCGCAGTTGCTCCTG+CGG 0.571832 4.4:-3841238 None:intergenic
ACTCACCCTACTTTGCTGGA+TGG 0.572647 4.4:+3840844 MS.gene20160:CDS
GGCTAATATGCTATCAGACA+AGG 0.573142 4.4:+3842263 MS.gene20160:CDS
TTGGAATTGAGTGTTTGAAA+GGG 0.574213 4.4:+3842367 MS.gene20160:CDS
GCTTTCCATCCAGCAAAGTA+GGG 0.580141 4.4:-3840849 None:intergenic
AGCTTCATGGAACAAATAAG+AGG 0.582469 4.4:+3841177 MS.gene20160:CDS
TAAATCATTCAGAAGTGCAA+TGG 0.583118 4.4:+3841152 MS.gene20160:CDS
TCTTGTCATTGTGATCAACC+TGG 0.587957 4.4:+3842513 MS.gene20160:CDS
ATCTCAGACGAAATCTACTC+GGG 0.596514 4.4:+3842018 MS.gene20160:CDS
TTCCTACCCCGTACTATCCA+GGG 0.606239 4.4:+3841307 MS.gene20160:CDS
TCAATGAACATGAAAGTGAG+TGG 0.607414 4.4:+3841895 MS.gene20160:CDS
ATTGTTCCAACTCTGAAACC+TGG 0.608322 4.4:-3842156 None:intergenic
GAAAGAATAGTCATCACCGC+AGG 0.624197 4.4:+3841222 MS.gene20160:CDS
AGTAGATTTCGTCTGAGATG+AGG 0.631875 4.4:-3842014 None:intergenic
TGAAATTGAATATATCTCCA+GGG 0.640695 4.4:+3842487 MS.gene20160:CDS
TTCATGGAACAAATAAGAGG+TGG 0.647531 4.4:+3841180 MS.gene20160:CDS
CATCACCGCAGGAGCAACTG+CGG 0.654637 4.4:+3841233 MS.gene20160:CDS
TACCCTGGATAGTACGGGGT+AGG 0.665259 4.4:-3841309 None:intergenic
GTACGGGGTAGGAACAAGCA+AGG 0.685560 4.4:-3841298 None:intergenic
AAACAGACCCTAAACCAACC+AGG 0.685588 4.4:-3842531 None:intergenic
TTCTTACCCTGGATAGTACG+GGG 0.696996 4.4:-3841313 None:intergenic
TCACAATGACAAGAACACCC+TGG 0.704106 4.4:-3842504 None:intergenic
GAACAAGCAAGGCATCGCCG+GGG 0.751656 4.4:-3841287 None:intergenic
GTACTTGGAAGAGCACCCAG+AGG 0.784244 4.4:+3841080 MS.gene20160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATATATTAAGATTAAT+AGG + chr4.4:3842295-3842314 MS.gene20160:CDS 10.0%
!! GAAATATATTAAGATTAATA+GGG + chr4.4:3842296-3842315 MS.gene20160:CDS 10.0%
!! AATTTATTCATACAATGATA+AGG + chr4.4:3842176-3842195 MS.gene20160:CDS 15.0%
!! AGATATGAAATGATTATTGA+AGG + chr4.4:3842333-3842352 MS.gene20160:CDS 20.0%
! AAATATGAGTGAACAAACTT+TGG + chr4.4:3842557-3842576 MS.gene20160:CDS 25.0%
! AGTTAGTTAACTCATCATAA+GGG - chr4.4:3840887-3840906 None:intergenic 25.0%
! CACTTCATCATAAAGAAAAA+AGG - chr4.4:3841618-3841637 None:intergenic 25.0%
! TATATTAATGTGTATTGTGC+AGG + chr4.4:3841035-3841054 MS.gene20160:intron 25.0%
! TGAAATTGAATATATCTCCA+GGG + chr4.4:3842487-3842506 MS.gene20160:CDS 25.0%
!! TCTTTTTCTTGTACTTTTGT+AGG + chr4.4:3841755-3841774 MS.gene20160:intron 25.0%
!!! ATATTACTTGAGAGTGTTTA+TGG - chr4.4:3841509-3841528 None:intergenic 25.0%
ATAGTTAGTTCCAAAACCAT+TGG - chr4.4:3841016-3841035 None:intergenic 30.0%
CTGAAATTGAATATATCTCC+AGG + chr4.4:3842486-3842505 MS.gene20160:CDS 30.0%
GAGTTAGTTAACTCATCATA+AGG - chr4.4:3840888-3840907 None:intergenic 30.0%
GCATTATTTCAAGACTATCA+TGG + chr4.4:3841126-3841145 MS.gene20160:CDS 30.0%
GTTGAACTGTTATACCTAAA+GGG - chr4.4:3841945-3841964 None:intergenic 30.0%
TCTTCAGGAGTTATACAAAT+GGG + chr4.4:3840906-3840925 MS.gene20160:CDS 30.0%
TGAAACCATTATAATCCTCT+GGG - chr4.4:3841098-3841117 None:intergenic 30.0%
TTATACCTAAAGGGTTTGAA+GGG - chr4.4:3841936-3841955 None:intergenic 30.0%
TTGAAACCATTATAATCCTC+TGG - chr4.4:3841099-3841118 None:intergenic 30.0%
TTGGAATTGAGTGTTTGAAA+GGG + chr4.4:3842367-3842386 MS.gene20160:CDS 30.0%
TTGTGTTTGTGAAGATATCA+AGG - chr4.4:3842235-3842254 None:intergenic 30.0%
! ATCATAAGGGTTTTCATCAT+AGG - chr4.4:3840874-3840893 None:intergenic 30.0%
! TAAATCATTCAGAAGTGCAA+TGG + chr4.4:3841152-3841171 MS.gene20160:CDS 30.0%
! TATAGTCTCTCTAAAGATCT+TGG + chr4.4:3842129-3842148 MS.gene20160:CDS 30.0%
! TTCACAAACACAACAGTTTT+TGG + chr4.4:3842242-3842261 MS.gene20160:CDS 30.0%
!! ACTATTTTTCATAACTCTCG+AGG - chr4.4:3841462-3841481 None:intergenic 30.0%
AAACCATTGGTGAACGAATT+AGG - chr4.4:3841003-3841022 None:intergenic 35.0%
AGCTTCATGGAACAAATAAG+AGG + chr4.4:3841177-3841196 MS.gene20160:CDS 35.0%
ATTGCAATTTCTGACACTCA+TGG + chr4.4:3840816-3840835 MS.gene20160:CDS 35.0%
CGTTGAACTGTTATACCTAA+AGG - chr4.4:3841946-3841965 None:intergenic 35.0%
CTCTTCAGGAGTTATACAAA+TGG + chr4.4:3840905-3840924 MS.gene20160:CDS 35.0%
GAATCAAGCACCATACAATA+CGG + chr4.4:3841333-3841352 MS.gene20160:intron 35.0%
GATGAGTTAACTAACTCTTC+AGG + chr4.4:3840891-3840910 MS.gene20160:CDS 35.0%
GTAGGTTTGATAGAGACTTA+AGG + chr4.4:3841773-3841792 MS.gene20160:intron 35.0%
GTTATACCTAAAGGGTTTGA+AGG - chr4.4:3841937-3841956 None:intergenic 35.0%
GTTGGAATTGAGTGTTTGAA+AGG + chr4.4:3842366-3842385 MS.gene20160:CDS 35.0%
TCAATGAACATGAAAGTGAG+TGG + chr4.4:3841895-3841914 MS.gene20160:CDS 35.0%
TGGTAGAGCTAAATTTGATC+CGG + chr4.4:3841200-3841219 MS.gene20160:CDS 35.0%
TTCATGGAACAAATAAGAGG+TGG + chr4.4:3841180-3841199 MS.gene20160:CDS 35.0%
! AATTCGTTCACCAATGGTTT+TGG + chr4.4:3841003-3841022 MS.gene20160:intron 35.0%
! ACAAACTTTGGAAGTTGCAT+TGG + chr4.4:3842569-3842588 MS.gene20160:CDS 35.0%
! ATTGAAGGCTTGAAAAGTGT+TGG + chr4.4:3842348-3842367 MS.gene20160:CDS 35.0%
! TTAGTTGACTCCTTCAAAAG+TGG - chr4.4:3842426-3842445 None:intergenic 35.0%
!! ACTTTTGAAGGAGTCAACTA+AGG + chr4.4:3842425-3842444 MS.gene20160:CDS 35.0%
!! GAATTTGAGTCCACTTTTGA+AGG + chr4.4:3842413-3842432 MS.gene20160:CDS 35.0%
AAGCTAGCTAGCTAGCTAAA+AGG - chr4.4:3841654-3841673 None:intergenic 40.0%
ACAAACCCTTCAAACCCTTT+AGG + chr4.4:3841928-3841947 MS.gene20160:CDS 40.0%
ACATATAGTCTACGCAAGCA+TGG - chr4.4:3841716-3841735 None:intergenic 40.0%
AGGAAGGTGAATTGGAACTT+TGG + chr4.4:3842445-3842464 MS.gene20160:CDS 40.0%
ATCTCAGACGAAATCTACTC+GGG + chr4.4:3842018-3842037 MS.gene20160:CDS 40.0%
ATTCTTACCCTGGATAGTAC+GGG - chr4.4:3841317-3841336 None:intergenic 40.0%
CATCTCAGACGAAATCTACT+CGG + chr4.4:3842017-3842036 MS.gene20160:CDS 40.0%
CCAAACATAGCCGTATTGTA+TGG - chr4.4:3841346-3841365 None:intergenic 40.0%
CCATACAATACGGCTATGTT+TGG + chr4.4:3841343-3841362 MS.gene20160:intron 40.0%
GATTCTTACCCTGGATAGTA+CGG - chr4.4:3841318-3841337 None:intergenic 40.0%
GGAAAGAATACGTAGCTTCA+TGG + chr4.4:3842590-3842609 MS.gene20160:CDS 40.0%
GGCTAATATGCTATCAGACA+AGG + chr4.4:3842263-3842282 MS.gene20160:CDS 40.0%
GGTTTGATAGAGACTTAAGG+TGG + chr4.4:3841776-3841795 MS.gene20160:intron 40.0%
GTCAACTAAGGAAGGTGAAT+TGG + chr4.4:3842437-3842456 MS.gene20160:CDS 40.0%
TCTAAAGATCTTGGTCTACC+AGG + chr4.4:3842138-3842157 MS.gene20160:CDS 40.0%
TCTTGTCATTGTGATCAACC+TGG + chr4.4:3842513-3842532 MS.gene20160:CDS 40.0%
TGTAAGCATAGCAGAGATTC+TGG + chr4.4:3842065-3842084 MS.gene20160:CDS 40.0%
TTGAAGGAGTCAACTAAGGA+AGG + chr4.4:3842429-3842448 MS.gene20160:CDS 40.0%
TTTGACCTGTTGCAGTGAAT+TGG - chr4.4:3841823-3841842 None:intergenic 40.0%
! AGTAGATTTCGTCTGAGATG+AGG - chr4.4:3842017-3842036 None:intergenic 40.0%
!! ATTGTTCCAACTCTGAAACC+TGG - chr4.4:3842159-3842178 None:intergenic 40.0%
!! CGACTTGGTAGAAAAGTACT+TGG + chr4.4:3841065-3841084 MS.gene20160:CDS 40.0%
!! GGAATGCAGGATTGTTTTGT+TGG + chr4.4:3842388-3842407 MS.gene20160:CDS 40.0%
!! TAAGCTGCTTCTAAAGCTTG+AGG - chr4.4:3841862-3841881 None:intergenic 40.0%
!! TGATCAACCTGGTTGGTTTA+GGG + chr4.4:3842524-3842543 MS.gene20160:CDS 40.0%
AAACAGACCCTAAACCAACC+AGG - chr4.4:3842534-3842553 None:intergenic 45.0%
AAGTGCAATGGCAAGCTTCA+TGG + chr4.4:3841164-3841183 MS.gene20160:CDS 45.0%
ACCTTCATTCTTGCAAACCC+CGG + chr4.4:3841270-3841289 MS.gene20160:CDS 45.0%
AGAGACTTAAGGTGGAGAAC+TGG + chr4.4:3841784-3841803 MS.gene20160:CDS 45.0%
ATGGTGCTTGATTCTTACCC+TGG - chr4.4:3841327-3841346 None:intergenic 45.0%
GAAAACTCACCCTACTTTGC+TGG + chr4.4:3840840-3840859 MS.gene20160:CDS 45.0%
GAAAGAATAGTCATCACCGC+AGG + chr4.4:3841222-3841241 MS.gene20160:CDS 45.0%
GAGCACCCAGAGGATTATAA+TGG + chr4.4:3841090-3841109 MS.gene20160:CDS 45.0%
GAGTGTTTGAAAGGGAATGC+AGG + chr4.4:3842375-3842394 MS.gene20160:CDS 45.0%
GCGGTGATGACTATTCTTTC+CGG - chr4.4:3841222-3841241 None:intergenic 45.0%
GCTCCTAATTCGTTCACCAA+TGG + chr4.4:3840997-3841016 MS.gene20160:intron 45.0%
GCTTTCCATCCAGCAAAGTA+GGG - chr4.4:3840852-3840871 None:intergenic 45.0%
GTCATTGTGATCAACCTGGT+TGG + chr4.4:3842517-3842536 MS.gene20160:CDS 45.0%
TAGTGCCAATTCACTGCAAC+AGG + chr4.4:3841815-3841834 MS.gene20160:CDS 45.0%
TCACAATGACAAGAACACCC+TGG - chr4.4:3842507-3842526 None:intergenic 45.0%
TTCTTACCCTGGATAGTACG+GGG - chr4.4:3841316-3841335 None:intergenic 45.0%
! AGATTTCGTCTGAGATGAGG+TGG - chr4.4:3842014-3842033 None:intergenic 45.0%
!! GTGATCAACCTGGTTGGTTT+AGG + chr4.4:3842523-3842542 MS.gene20160:CDS 45.0%
ACTCACCCTACTTTGCTGGA+TGG + chr4.4:3840844-3840863 MS.gene20160:CDS 50.0%
AGACGAAATCTACTCGGGCT+CGG + chr4.4:3842023-3842042 MS.gene20160:CDS 50.0%
GGCTTTCCATCCAGCAAAGT+AGG - chr4.4:3840853-3840872 None:intergenic 50.0%
GGTCTACCAGGTTTCAGAGT+TGG + chr4.4:3842150-3842169 MS.gene20160:CDS 50.0%
TGTGCAGGTTTCGTTCGACT+TGG + chr4.4:3841050-3841069 MS.gene20160:intron 50.0%
TTCCTACCCCGTACTATCCA+GGG + chr4.4:3841307-3841326 MS.gene20160:CDS 50.0%
ATTAGCCGCAGTTGCTCCTG+CGG - chr4.4:3841241-3841260 None:intergenic 55.0%
GCCGGGGTTTGCAAGAATGA+AGG - chr4.4:3841274-3841293 None:intergenic 55.0%
GTACGGGGTAGGAACAAGCA+AGG - chr4.4:3841301-3841320 None:intergenic 55.0%
GTTCCTACCCCGTACTATCC+AGG + chr4.4:3841306-3841325 MS.gene20160:CDS 55.0%
TACCCTGGATAGTACGGGGT+AGG - chr4.4:3841312-3841331 None:intergenic 55.0%
!! AGGAACAAGCAAGGCATCGC+CGG - chr4.4:3841292-3841311 None:intergenic 55.0%
!! GTACTTGGAAGAGCACCCAG+AGG + chr4.4:3841080-3841099 MS.gene20160:CDS 55.0%
CATCACCGCAGGAGCAACTG+CGG + chr4.4:3841233-3841252 MS.gene20160:CDS 60.0%
!! GAACAAGCAAGGCATCGCCG+GGG - chr4.4:3841290-3841309 None:intergenic 60.0%
!! GGAACAAGCAAGGCATCGCC+GGG - chr4.4:3841291-3841310 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3840774 3842641 3840774 ID=MS.gene20160
chr4.4 mRNA 3840774 3842641 3840774 ID=MS.gene20160.t1;Parent=MS.gene20160
chr4.4 exon 3840774 3840944 3840774 ID=MS.gene20160.t1.exon1;Parent=MS.gene20160.t1
chr4.4 CDS 3840774 3840944 3840774 ID=cds.MS.gene20160.t1;Parent=MS.gene20160.t1
chr4.4 exon 3841057 3841328 3841057 ID=MS.gene20160.t1.exon2;Parent=MS.gene20160.t1
chr4.4 CDS 3841057 3841328 3841057 ID=cds.MS.gene20160.t1;Parent=MS.gene20160.t1
chr4.4 exon 3841777 3842641 3841777 ID=MS.gene20160.t1.exon3;Parent=MS.gene20160.t1
chr4.4 CDS 3841777 3842641 3841777 ID=cds.MS.gene20160.t1;Parent=MS.gene20160.t1
Gene Sequence

>MS.gene20160

ATGGGTATTGAGATAGAACAATCTTGTGTACAACTTTCAAACATTGCAATTTCTGACACTCATGGAGAAAACTCACCCTACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATGATGAGTTAACTAACTCTTCAGGAGTTATACAAATGGGATTAGCAGAAAATCAAGTATGTTTATATATCTTGAATTAATAAGTCTTATTATGTCTATATATAATTAGCTCCTAATTCGTTCACCAATGGTTTTGGAACTAACTATATATTAATGTGTATTGTGCAGGTTTCGTTCGACTTGGTAGAAAAGTACTTGGAAGAGCACCCAGAGGATTATAATGGTTTCAAAGAGAATGCATTATTTCAAGACTATCATGGACTTAAATCATTCAGAAGTGCAATGGCAAGCTTCATGGAACAAATAAGAGGTGGTAGAGCTAAATTTGATCCGGAAAGAATAGTCATCACCGCAGGAGCAACTGCGGCTAATGAGCTTTTAACCTTCATTCTTGCAAACCCCGGCGATGCCTTGCTTGTTCCTACCCCGTACTATCCAGGGTAAGAATCAAGCACCATACAATACGGCTATGTTTGGATAAACAATTTAATTGATAAACTATGTCTATAATACATGATAAAATACAACTAAACTATTTTATATAAGTTATAAATTGTTTTCACTAGCATTCCTCGAGAGTTATGAAAAATAGTTTAAGCAGAAGTCATAAGTTGTTTCCATAAACACTCTCAAGTAATATCAGTAGATAAATTCAAATAAATCAATTCAAAAAGAGTGCATATGTCTTAATAACACGCGTAAAATTTCACACATTTATTTCATGCACCTTTTTTCTTTATGATGAAGTGCTATTATTAAAAACCTTTTAGCTAGCTAGCTAGCTTCATTTGCTTTAATTTATCATTAGAAGCTATAGTATGTAACCATGCTTGCGTAGACTATATGTGATGAAATAATCTCATGTATCTTTTTCTTGTACTTTTGTAGGTTTGATAGAGACTTAAGGTGGAGAACTGGTGTAAACATAGTGCCAATTCACTGCAACAGGTCAAACAATTTTCAAATTACACCTCAAGCTTTAGAAGCAGCTTACAAAGAAGCAGAATCAATGAACATGAAAGTGAGTGGAGTACTCATCACAAACCCTTCAAACCCTTTAGGTATAACAGTTCAACGCACAGTTCTTGAAGAGATTCTTGACTTTGTGACTAAAAAGAACATCCACCTCATCTCAGACGAAATCTACTCGGGCTCGGTCTTTAATTCATCTGAGTTTGTAAGCATAGCAGAGATTCTGGAAGCTCGTCGATACAAAGATGCAGAGAGAGTTCACATTGTTTATAGTCTCTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTTGGAACAATTTATTCATACAATGATAAGGTTGTAACAACAGCGCGAAGAATGTCGAGTTTTACCTTGATATCTTCACAAACACAACAGTTTTTGGCTAATATGCTATCAGACAAGGAGTTTACAGAGAAATATATTAAGATTAATAGGGAGAGATTAAAGAAGAGATATGAAATGATTATTGAAGGCTTGAAAAGTGTTGGAATTGAGTGTTTGAAAGGGAATGCAGGATTGTTTTGTTGGATGAATTTGAGTCCACTTTTGAAGGAGTCAACTAAGGAAGGTGAATTGGAACTTTGGAATGTTGTTTTGAATGAACTGAAATTGAATATATCTCCAGGGTGTTCTTGTCATTGTGATCAACCTGGTTGGTTTAGGGTCTGTTTTGCAAATATGAGTGAACAAACTTTGGAAGTTGCATTGGAAAGAATACGTAGCTTCATGGCTAGAAGAATAATGACAAAGAAGATATAA
Protein sequence

>MS.gene20160.t1

MGIEIEQSCVQLSNIAISDTHGENSPYFAGWKAYDENPYDELTNSSGVIQMGLAENQVSFDLVEKYLEEHPEDYNGFKENALFQDYHGLKSFRSAMASFMEQIRGGRAKFDPERIVITAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCNRSNNFQITPQALEAAYKEAESMNMKVSGVLITNPSNPLGITVQRTVLEEILDFVTKKNIHLISDEIYSGSVFNSSEFVSIAEILEARRYKDAERVHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQQFLANMLSDKEFTEKYIKINRERLKKRYEMIIEGLKSVGIECLKGNAGLFCWMNLSPLLKESTKEGELELWNVVLNELKLNISPGCSCHCDQPGWFRVCFANMSEQTLEVALERIRSFMARRIMTKKI