AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20161

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20161.t1 MTR_8g101750 95.402 435 20 0 1 435 1 435 0.0 875
MS.gene20161.t1 MTR_8g101820 89.195 435 34 2 1 435 1 422 0.0 808
MS.gene20161.t1 MTR_6g091760 81.007 437 75 2 1 429 1 437 0.0 756
MS.gene20161.t1 MTR_5g015020 58.661 433 162 6 12 428 4 435 0.0 539
MS.gene20161.t1 MTR_4g097540 57.666 437 167 5 12 431 4 439 0.0 538
MS.gene20161.t1 MTR_3g103550 56.744 430 175 3 12 430 2 431 0.0 530
MS.gene20161.t1 MTR_8g098930 56.706 425 175 2 12 427 12 436 0.0 523
MS.gene20161.t1 MTR_8g028600 55.478 429 183 2 12 433 11 438 0.0 521
MS.gene20161.t1 MTR_7g079080 55.944 429 182 1 12 433 11 439 0.0 520
MS.gene20161.t1 MTR_6g463260 44.731 427 227 2 10 428 102 527 6.15e-145 424
MS.gene20161.t1 MTR_7g079080 55.689 334 148 0 100 433 1 334 1.16e-141 410
MS.gene20161.t1 MTR_5g011400 43.735 407 219 3 24 422 84 488 1.08e-130 386
MS.gene20161.t1 MTR_2g090730 56.757 74 28 1 77 150 31 100 3.02e-21 91.7
MS.gene20161.t1 MTR_8g091280 23.192 401 265 15 46 430 97 470 9.51e-17 82.4
MS.gene20161.t1 MTR_5g033230 24.272 309 213 12 83 373 103 408 3.95e-16 80.5
MS.gene20161.t1 MTR_5g033230 24.272 309 213 12 83 373 103 408 5.29e-16 80.5
MS.gene20161.t1 MTR_4g011820 23.101 316 211 11 115 423 92 382 2.24e-15 77.8
MS.gene20161.t1 MTR_2g090615 26.087 276 167 9 88 350 86 337 2.10e-14 75.1
MS.gene20161.t1 MTR_8g023140 22.102 371 261 14 78 425 149 514 5.52e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20161.t1 AT4G26200 75.291 429 97 3 10 429 16 444 0.0 662
MS.gene20161.t1 AT4G37770 59.122 433 167 2 12 434 4 436 0.0 553
MS.gene20161.t1 AT4G08040 57.674 430 175 1 12 434 2 431 0.0 546
MS.gene20161.t1 AT5G65800 57.944 428 170 3 11 428 3 430 0.0 533
MS.gene20161.t1 AT3G49700 58.042 429 168 3 11 428 3 430 0.0 528
MS.gene20161.t1 AT2G22810 55.734 436 182 3 10 434 2 437 0.0 525
MS.gene20161.t1 AT3G61510 56.161 422 175 2 12 426 11 429 0.0 524
MS.gene20161.t1 AT4G11280 55.916 431 183 1 4 427 7 437 0.0 518
MS.gene20161.t1 AT1G01480 51.716 437 201 3 1 427 1 437 2.03e-172 493
MS.gene20161.t1 AT5G51690 44.028 427 230 3 10 428 67 492 1.10e-138 407
MS.gene20161.t1 AT1G01480 52.568 331 154 2 100 427 1 331 4.15e-134 391
MS.gene20161.t1 AT1G62960 43.210 405 227 3 22 423 143 547 2.16e-127 380
MS.gene20161.t1 AT5G51690 46.839 348 184 1 81 428 23 369 3.66e-124 365
MS.gene20161.t1 AT4G23600 25.627 359 230 13 88 433 78 412 4.10e-18 86.3
MS.gene20161.t1 AT5G36160 24.022 358 231 13 83 425 80 411 1.27e-17 85.1
MS.gene20161.t1 AT1G23310 25.271 277 189 9 83 344 103 376 1.44e-17 85.1
MS.gene20161.t1 AT1G23310 25.271 277 189 9 83 344 103 376 1.72e-17 85.1
MS.gene20161.t1 AT1G72330 22.691 379 265 14 73 428 160 533 3.69e-17 84.0
MS.gene20161.t1 AT1G70580 24.910 277 190 10 83 344 103 376 9.98e-17 82.4
MS.gene20161.t1 AT1G70580 24.910 277 190 10 83 344 103 376 9.98e-17 82.4
MS.gene20161.t1 AT1G70580 24.910 277 190 10 83 344 103 376 9.98e-17 82.4
MS.gene20161.t1 AT1G70580 24.910 277 190 10 83 344 103 376 9.98e-17 82.4
MS.gene20161.t1 AT1G72330 23.596 267 190 8 73 328 160 423 2.26e-16 81.3
MS.gene20161.t1 AT1G72330 21.939 392 265 14 73 428 160 546 2.11e-15 78.6
MS.gene20161.t1 AT1G80360 23.485 396 250 15 41 424 28 382 3.34e-15 77.4
MS.gene20161.t1 AT1G80360 23.485 396 250 15 41 424 28 382 3.34e-15 77.4
MS.gene20161.t1 AT1G80360 23.485 396 250 15 41 424 28 382 3.34e-15 77.4
MS.gene20161.t1 AT1G80360 23.485 396 250 15 41 424 28 382 3.34e-15 77.4
MS.gene20161.t1 AT2G20610 24.507 355 223 14 88 425 109 435 3.65e-15 77.8
MS.gene20161.t1 AT4G23600 25.455 330 211 12 117 433 1 308 5.72e-15 75.9
MS.gene20161.t1 AT1G17290 22.259 301 214 11 78 362 168 464 1.29e-14 76.3
MS.gene20161.t1 AT4G23600 25.472 318 203 11 88 392 78 374 1.49e-14 75.5
MS.gene20161.t1 AT1G77670 24.340 341 233 11 87 424 116 434 1.49e-14 75.9
MS.gene20161.t1 AT2G20610 25.461 271 167 9 88 345 109 357 3.96e-14 74.3
MS.gene20161.t1 AT4G28410 26.619 278 163 11 88 350 111 362 9.25e-12 67.0
MS.gene20161.t1 AT4G28410 26.642 274 168 10 88 350 111 362 1.04e-11 67.0
MS.gene20161.t1 AT4G23590 23.851 348 234 12 88 425 78 404 2.87e-11 65.5
MS.gene20161.t1 AT5G53970 23.416 363 233 14 88 433 76 410 4.89e-11 64.7

Find 88 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATATATTAAGATTAAT+AGG 0.143027 4.4:+3849594 MS.gene20161:CDS
GCGGTGATGACTATTCTTTC+TGG 0.188225 4.4:-3848644 None:intergenic
GTGATCAACCTGGTTGGTTT+AGG 0.197998 4.4:+3849822 MS.gene20161:CDS
TGATCAACCTGGTTGGTTTA+GGG 0.271865 4.4:+3849823 MS.gene20161:CDS
AACTTCATGGCTAGCCGAAC+AGG 0.317741 4.4:+3849902 MS.gene20161:CDS
ACAAACCCTTCAAACCCTTT+AGG 0.351926 4.4:+3849227 MS.gene20161:CDS
GTCAACTAAGGAAGGTGAAT+TGG 0.354677 4.4:+3849736 MS.gene20161:CDS
GATGAATTAACTAACTCTTC+AGG 0.373544 4.4:+3848310 MS.gene20161:CDS
TGTGCAGGTTTCATTTGATT+TGG 0.377676 4.4:+3848475 MS.gene20161:intron
GAAAACTCACCCTACTTTGC+TGG 0.379915 4.4:+3848259 MS.gene20161:CDS
GAGTTAGTTAATTCATCATA+AGG 0.381161 4.4:-3848304 None:intergenic
GATTCTTACCCTGGATAGTA+AGG 0.392460 4.4:-3848740 None:intergenic
TATAGTCTCTCTAAAGATCT+TGG 0.393148 4.4:+3849428 MS.gene20161:CDS
CTCATTAGCCGCAGTTGCTC+CGG 0.395906 4.4:-3848666 None:intergenic
ACATCTTCTTTGTCCCTGTT+CGG 0.397573 4.4:-3849916 None:intergenic
GAAATATATTAAGATTAATA+GGG 0.399795 4.4:+3849595 MS.gene20161:CDS
AGGAACAAGCAAGGCATCGC+CGG 0.403862 4.4:-3848714 None:intergenic
CTGAAACCATTATAATCCTC+AGG 0.404711 4.4:-3848521 None:intergenic
AAGTGCAATGGCAAGTTTCA+TGG 0.405411 4.4:+3848589 MS.gene20161:CDS
GAGCACCCTGAGGATTATAA+TGG 0.406179 4.4:+3848515 MS.gene20161:CDS
GTTCCTACCCCTTACTATCC+AGG 0.407257 4.4:+3848731 MS.gene20161:CDS
GGAAAGAATACGTAACTTCA+TGG 0.412992 4.4:+3849889 MS.gene20161:CDS
TTTGACCTGTTGCAGTGAAT+TGG 0.417511 4.4:-3849119 None:intergenic
CGTTGAACTGTTATACCTAA+AGG 0.428028 4.4:-3849242 None:intergenic
GTTGAACTGTTATACCTAAA+GGG 0.430255 4.4:-3849241 None:intergenic
GTTATACCTAAAGGGTTTGA+AGG 0.431593 4.4:-3849233 None:intergenic
AATTTATTCATACAATGATA+AGG 0.432216 4.4:+3849475 MS.gene20161:CDS
AGATATGAAATGATTATTGA+AGG 0.438836 4.4:+3849632 MS.gene20161:CDS
TAGTGCCAATTCACTGCAAC+AGG 0.441325 4.4:+3849114 MS.gene20161:CDS
CTGAAATTGAATATATCTCC+AGG 0.445529 4.4:+3849785 MS.gene20161:CDS
GTAGGTTTGATAGAGACTTA+AGG 0.446910 4.4:+3849072 MS.gene20161:intron
GGAACAAGCAAGGCATCGCC+GGG 0.449445 4.4:-3848713 None:intergenic
GCATTATTTCAAGACTATCA+TGG 0.449699 4.4:+3848551 MS.gene20161:CDS
ATTGAAGGCTTGAAAAGTGT+TGG 0.452654 4.4:+3849647 MS.gene20161:CDS
TTAGTTGACTCCTTCAAAAG+TGG 0.458012 4.4:-3849722 None:intergenic
GAGTGTTTGAAAGGGAATGC+AGG 0.465675 4.4:+3849674 MS.gene20161:CDS
AGTTAGTTAATTCATCATAA+GGG 0.468946 4.4:-3848303 None:intergenic
AGGAAGGTGAATTGGAACTT+TGG 0.476087 4.4:+3849744 MS.gene20161:CDS
ACAAACTTTGGATGTTGCAT+TGG 0.480931 4.4:+3849868 MS.gene20161:CDS
GGTCTACCAGGTTTCAGAGT+TGG 0.483669 4.4:+3849449 MS.gene20161:CDS
AAATATGAGTGAACAAACTT+TGG 0.486245 4.4:+3849856 MS.gene20161:CDS
CTCTTCAGGAGTTATACAAA+TGG 0.488431 4.4:+3848324 MS.gene20161:CDS
AGAGACTTAAGGTGGAGAAC+TGG 0.493331 4.4:+3849083 MS.gene20161:CDS
CATCTCAGACGAAATCTACT+CGG 0.501179 4.4:+3849316 MS.gene20161:CDS
GGCTTTCCATCCAGCAAAGT+AGG 0.501359 4.4:-3848269 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.510586 4.4:+3849437 MS.gene20161:CDS
ACAGGGACAAAGAAGATGTA+AGG 0.520984 4.4:+3849920 MS.gene20161:CDS
TTGTGTTTGTGAAGATATCA+TGG 0.525948 4.4:-3849531 None:intergenic
GCCGGGGTTTGCAAGAATGA+AGG 0.526652 4.4:-3848696 None:intergenic
ACCTTCATTCTTGCAAACCC+CGG 0.529279 4.4:+3848695 MS.gene20161:CDS
ATTGCAATTTCTGACACTCA+TGG 0.531513 4.4:+3848235 MS.gene20161:CDS
TCTTCAGGAGTTATACAAAT+GGG 0.535454 4.4:+3848325 MS.gene20161:CDS
GTCATTGTGATCAACCTGGT+TGG 0.538197 4.4:+3849816 MS.gene20161:CDS
TTCCTACCCCTTACTATCCA+GGG 0.542899 4.4:+3848732 MS.gene20161:CDS
TTATACCTAAAGGGTTTGAA+GGG 0.548536 4.4:-3849232 None:intergenic
ATTCTTACCCTGGATAGTAA+GGG 0.548613 4.4:-3848739 None:intergenic
TAAGCTGCTTCTAAAGCTTG+AGG 0.550801 4.4:-3849158 None:intergenic
AGACGAAATCTACTCGGGCT+CGG 0.552466 4.4:+3849322 MS.gene20161:CDS
GGTTTGATAGAGACTTAAGG+TGG 0.553899 4.4:+3849075 MS.gene20161:intron
TTGAAGGAGTCAACTAAGGA+AGG 0.555608 4.4:+3849728 MS.gene20161:CDS
AGATTTCGTCTGAGATGAGG+TGG 0.558059 4.4:-3849310 None:intergenic
GTTGGAATTGAGTGTTTGAA+AGG 0.569169 4.4:+3849665 MS.gene20161:CDS
ACTCACCCTACTTTGCTGGA+TGG 0.572647 4.4:+3848263 MS.gene20161:CDS
GGCTAATATGCTATCAGACA+AGG 0.573142 4.4:+3849562 MS.gene20161:CDS
TTGGAATTGAGTGTTTGAAA+GGG 0.574213 4.4:+3849666 MS.gene20161:CDS
AGTTTCATGGAACAAATAAG+AGG 0.575774 4.4:+3848602 MS.gene20161:CDS
GCTTTCCATCCAGCAAAGTA+GGG 0.580141 4.4:-3848268 None:intergenic
AGGAGTTATACAAATGGGAC+TGG 0.580638 4.4:+3848330 MS.gene20161:CDS
TAAATCATTCAGAAGTGCAA+TGG 0.583118 4.4:+3848577 MS.gene20161:CDS
TCTTGTCATTGTGATCAACC+TGG 0.587957 4.4:+3849812 MS.gene20161:CDS
ATTAGCCGCAGTTGCTCCGG+CGG 0.589538 4.4:-3848663 None:intergenic
CATCACCGCCGGAGCAACTG+CGG 0.595084 4.4:+3848658 MS.gene20161:CDS
ATCTCAGACGAAATCTACTC+GGG 0.596514 4.4:+3849317 MS.gene20161:CDS
TTCTTACCCTGGATAGTAAG+GGG 0.604583 4.4:-3848738 None:intergenic
TCAATGAACATGAAAGTGAG+TGG 0.607414 4.4:+3849194 MS.gene20161:CDS
ATTGTTCCAACTCTGAAACC+TGG 0.608322 4.4:-3849455 None:intergenic
TGAAACCATTATAATCCTCA+GGG 0.627551 4.4:-3848520 None:intergenic
ACTTCATGGCTAGCCGAACA+GGG 0.629105 4.4:+3849903 MS.gene20161:CDS
GAAAGAATAGTCATCACCGC+CGG 0.631099 4.4:+3848647 MS.gene20161:CDS
AGTAGATTTCGTCTGAGATG+AGG 0.631875 4.4:-3849313 None:intergenic
TACCCTGGATAGTAAGGGGT+AGG 0.633960 4.4:-3848734 None:intergenic
TGAAATTGAATATATCTCCA+GGG 0.640695 4.4:+3849786 MS.gene20161:CDS
TTCATGGAACAAATAAGAGG+TGG 0.647531 4.4:+3848605 MS.gene20161:CDS
GTAAGGGGTAGGAACAAGCA+AGG 0.672268 4.4:-3848723 None:intergenic
AAACAGACCCTAAACCAACC+AGG 0.685588 4.4:-3849830 None:intergenic
TCACAATGACAAGAACACCC+TGG 0.704106 4.4:-3849803 None:intergenic
GAACAAGCAAGGCATCGCCG+GGG 0.751656 4.4:-3848712 None:intergenic
GTACTTGAAAGAGCACCCTG+AGG 0.755804 4.4:+3848505 MS.gene20161:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGAACTAATTAATT+AGG - chr4.4:3848420-3848439 None:intergenic 10.0%
!! AGAAATATATTAAGATTAAT+AGG + chr4.4:3849594-3849613 MS.gene20161:CDS 10.0%
!! GAAATATATTAAGATTAATA+GGG + chr4.4:3849595-3849614 MS.gene20161:CDS 10.0%
!! TTAATTAGTTCATTAATTAA+TGG + chr4.4:3848422-3848441 MS.gene20161:intron 10.0%
!! AATTTATTCATACAATGATA+AGG + chr4.4:3849475-3849494 MS.gene20161:CDS 15.0%
!!! ATATAATCATACTTGATTTT+CGG - chr4.4:3848356-3848375 None:intergenic 15.0%
!! AGATATGAAATGATTATTGA+AGG + chr4.4:3849632-3849651 MS.gene20161:CDS 20.0%
!! AGTTAGTTAATTCATCATAA+GGG - chr4.4:3848306-3848325 None:intergenic 20.0%
!!! AGTTCATTAATTAATGGTTT+TGG + chr4.4:3848428-3848447 MS.gene20161:intron 20.0%
! AAATATGAGTGAACAAACTT+TGG + chr4.4:3849856-3849875 MS.gene20161:CDS 25.0%
! GAGTTAGTTAATTCATCATA+AGG - chr4.4:3848307-3848326 None:intergenic 25.0%
! TATATTAATGTGTATTGTGC+AGG + chr4.4:3848460-3848479 MS.gene20161:intron 25.0%
! TGAAATTGAATATATCTCCA+GGG + chr4.4:3849786-3849805 MS.gene20161:CDS 25.0%
!! TTAATTTTGAATAACTCCTG+AGG - chr4.4:3848867-3848886 None:intergenic 25.0%
!!! ATTTTACTTGAGAGTGTTTA+TGG - chr4.4:3848919-3848938 None:intergenic 25.0%
AGTTTCATGGAACAAATAAG+AGG + chr4.4:3848602-3848621 MS.gene20161:CDS 30.0%
CTGAAATTGAATATATCTCC+AGG + chr4.4:3849785-3849804 MS.gene20161:CDS 30.0%
GATGAATTAACTAACTCTTC+AGG + chr4.4:3848310-3848329 MS.gene20161:CDS 30.0%
GCATTATTTCAAGACTATCA+TGG + chr4.4:3848551-3848570 MS.gene20161:CDS 30.0%
GTTGAACTGTTATACCTAAA+GGG - chr4.4:3849244-3849263 None:intergenic 30.0%
TCTTCAGGAGTTATACAAAT+GGG + chr4.4:3848325-3848344 MS.gene20161:CDS 30.0%
TGAAACCATTATAATCCTCA+GGG - chr4.4:3848523-3848542 None:intergenic 30.0%
TTATACCTAAAGGGTTTGAA+GGG - chr4.4:3849235-3849254 None:intergenic 30.0%
TTGGAATTGAGTGTTTGAAA+GGG + chr4.4:3849666-3849685 MS.gene20161:CDS 30.0%
TTGTGTTTGTGAAGATATCA+TGG - chr4.4:3849534-3849553 None:intergenic 30.0%
! ATCATAAGGGTTTTCATCAT+AGG - chr4.4:3848293-3848312 None:intergenic 30.0%
! TAAATCATTCAGAAGTGCAA+TGG + chr4.4:3848577-3848596 MS.gene20161:CDS 30.0%
! TATAGTCTCTCTAAAGATCT+TGG + chr4.4:3849428-3849447 MS.gene20161:CDS 30.0%
! TATTTTCATCAACTATCCTC+AGG + chr4.4:3848848-3848867 MS.gene20161:intron 30.0%
! TCTTTTGCTTGTACTTTTGT+AGG + chr4.4:3849054-3849073 MS.gene20161:intron 30.0%
! TTCACAAACACAACAGTTTT+TGG + chr4.4:3849541-3849560 MS.gene20161:CDS 30.0%
ACAAACTTTGGATGTTGCAT+TGG + chr4.4:3849868-3849887 MS.gene20161:CDS 35.0%
ATATATAGTCTACGCAAGCA+TGG - chr4.4:3849015-3849034 None:intergenic 35.0%
ATTCTTACCCTGGATAGTAA+GGG - chr4.4:3848742-3848761 None:intergenic 35.0%
ATTGCAATTTCTGACACTCA+TGG + chr4.4:3848235-3848254 MS.gene20161:CDS 35.0%
CGTTGAACTGTTATACCTAA+AGG - chr4.4:3849245-3849264 None:intergenic 35.0%
CTCTTCAGGAGTTATACAAA+TGG + chr4.4:3848324-3848343 MS.gene20161:CDS 35.0%
CTGAAACCATTATAATCCTC+AGG - chr4.4:3848524-3848543 None:intergenic 35.0%
GGAAAGAATACGTAACTTCA+TGG + chr4.4:3849889-3849908 MS.gene20161:CDS 35.0%
GTAGGTTTGATAGAGACTTA+AGG + chr4.4:3849072-3849091 MS.gene20161:intron 35.0%
GTTATACCTAAAGGGTTTGA+AGG - chr4.4:3849236-3849255 None:intergenic 35.0%
GTTGGAATTGAGTGTTTGAA+AGG + chr4.4:3849665-3849684 MS.gene20161:CDS 35.0%
GTTGTTTATCCAAACATAGC+CGG - chr4.4:3848780-3848799 None:intergenic 35.0%
TCAATGAACATGAAAGTGAG+TGG + chr4.4:3849194-3849213 MS.gene20161:CDS 35.0%
TGTGCAGGTTTCATTTGATT+TGG + chr4.4:3848475-3848494 MS.gene20161:intron 35.0%
TTCATGGAACAAATAAGAGG+TGG + chr4.4:3848605-3848624 MS.gene20161:CDS 35.0%
! ATTGAAGGCTTGAAAAGTGT+TGG + chr4.4:3849647-3849666 MS.gene20161:CDS 35.0%
! TTAGTTGACTCCTTCAAAAG+TGG - chr4.4:3849725-3849744 None:intergenic 35.0%
!! ACTTTTGAAGGAGTCAACTA+AGG + chr4.4:3849724-3849743 MS.gene20161:CDS 35.0%
!! GAATTTGAGTCCACTTTTGA+AGG + chr4.4:3849712-3849731 MS.gene20161:CDS 35.0%
AAGTGCAATGGCAAGTTTCA+TGG + chr4.4:3848589-3848608 MS.gene20161:CDS 40.0%
AATGAAGCTAGCTAGCTAGA+AGG - chr4.4:3848957-3848976 None:intergenic 40.0%
ACAAACCCTTCAAACCCTTT+AGG + chr4.4:3849227-3849246 MS.gene20161:CDS 40.0%
ACATCTTCTTTGTCCCTGTT+CGG - chr4.4:3849919-3849938 None:intergenic 40.0%
AGGAAGGTGAATTGGAACTT+TGG + chr4.4:3849744-3849763 MS.gene20161:CDS 40.0%
AGGAGTTATACAAATGGGAC+TGG + chr4.4:3848330-3848349 MS.gene20161:CDS 40.0%
ATCTCAGACGAAATCTACTC+GGG + chr4.4:3849317-3849336 MS.gene20161:CDS 40.0%
CATCTCAGACGAAATCTACT+CGG + chr4.4:3849316-3849335 MS.gene20161:CDS 40.0%
GAATCAAGCACCATACAATC+CGG + chr4.4:3848758-3848777 MS.gene20161:intron 40.0%
GATTCTTACCCTGGATAGTA+AGG - chr4.4:3848743-3848762 None:intergenic 40.0%
GGCTAATATGCTATCAGACA+AGG + chr4.4:3849562-3849581 MS.gene20161:CDS 40.0%
GGTTTGATAGAGACTTAAGG+TGG + chr4.4:3849075-3849094 MS.gene20161:intron 40.0%
GTCAACTAAGGAAGGTGAAT+TGG + chr4.4:3849736-3849755 MS.gene20161:CDS 40.0%
TCTAAAGATCTTGGTCTACC+AGG + chr4.4:3849437-3849456 MS.gene20161:CDS 40.0%
TCTTGTCATTGTGATCAACC+TGG + chr4.4:3849812-3849831 MS.gene20161:CDS 40.0%
TTCTTACCCTGGATAGTAAG+GGG - chr4.4:3848741-3848760 None:intergenic 40.0%
TTGAAGGAGTCAACTAAGGA+AGG + chr4.4:3849728-3849747 MS.gene20161:CDS 40.0%
TTTGACCTGTTGCAGTGAAT+TGG - chr4.4:3849122-3849141 None:intergenic 40.0%
! AGTAGATTTCGTCTGAGATG+AGG - chr4.4:3849316-3849335 None:intergenic 40.0%
!! ATTGTTCCAACTCTGAAACC+TGG - chr4.4:3849458-3849477 None:intergenic 40.0%
!! GGAATGCAGGATTGTTTTGT+TGG + chr4.4:3849687-3849706 MS.gene20161:CDS 40.0%
!! TAAGCTGCTTCTAAAGCTTG+AGG - chr4.4:3849161-3849180 None:intergenic 40.0%
!! TGATCAACCTGGTTGGTTTA+GGG + chr4.4:3849823-3849842 MS.gene20161:CDS 40.0%
AAACAGACCCTAAACCAACC+AGG - chr4.4:3849833-3849852 None:intergenic 45.0%
ACCTTCATTCTTGCAAACCC+CGG + chr4.4:3848695-3848714 MS.gene20161:CDS 45.0%
AGAGACTTAAGGTGGAGAAC+TGG + chr4.4:3849083-3849102 MS.gene20161:CDS 45.0%
ATGGTGCTTGATTCTTACCC+TGG - chr4.4:3848752-3848771 None:intergenic 45.0%
CCAAACATAGCCGGATTGTA+TGG - chr4.4:3848771-3848790 None:intergenic 45.0%
CCATACAATCCGGCTATGTT+TGG + chr4.4:3848768-3848787 MS.gene20161:intron 45.0%
GAAAACTCACCCTACTTTGC+TGG + chr4.4:3848259-3848278 MS.gene20161:CDS 45.0%
GAAAGAATAGTCATCACCGC+CGG + chr4.4:3848647-3848666 MS.gene20161:CDS 45.0%
GAGCACCCTGAGGATTATAA+TGG + chr4.4:3848515-3848534 MS.gene20161:CDS 45.0%
GAGTGTTTGAAAGGGAATGC+AGG + chr4.4:3849674-3849693 MS.gene20161:CDS 45.0%
GCGGTGATGACTATTCTTTC+TGG - chr4.4:3848647-3848666 None:intergenic 45.0%
GCTTTCCATCCAGCAAAGTA+GGG - chr4.4:3848271-3848290 None:intergenic 45.0%
GTCATTGTGATCAACCTGGT+TGG + chr4.4:3849816-3849835 MS.gene20161:CDS 45.0%
TAGTGCCAATTCACTGCAAC+AGG + chr4.4:3849114-3849133 MS.gene20161:CDS 45.0%
TCACAATGACAAGAACACCC+TGG - chr4.4:3849806-3849825 None:intergenic 45.0%
TTCCTACCCCTTACTATCCA+GGG + chr4.4:3848732-3848751 MS.gene20161:CDS 45.0%
! AGATTTCGTCTGAGATGAGG+TGG - chr4.4:3849313-3849332 None:intergenic 45.0%
!! GTGATCAACCTGGTTGGTTT+AGG + chr4.4:3849822-3849841 MS.gene20161:CDS 45.0%
ACTCACCCTACTTTGCTGGA+TGG + chr4.4:3848263-3848282 MS.gene20161:CDS 50.0%
AGACGAAATCTACTCGGGCT+CGG + chr4.4:3849322-3849341 MS.gene20161:CDS 50.0%
GGCTTTCCATCCAGCAAAGT+AGG - chr4.4:3848272-3848291 None:intergenic 50.0%
GGTCTACCAGGTTTCAGAGT+TGG + chr4.4:3849449-3849468 MS.gene20161:CDS 50.0%
GTAAGGGGTAGGAACAAGCA+AGG - chr4.4:3848726-3848745 None:intergenic 50.0%
GTTCCTACCCCTTACTATCC+AGG + chr4.4:3848731-3848750 MS.gene20161:CDS 50.0%
TACCCTGGATAGTAAGGGGT+AGG - chr4.4:3848737-3848756 None:intergenic 50.0%
! AACTTCATGGCTAGCCGAAC+AGG + chr4.4:3849902-3849921 MS.gene20161:CDS 50.0%
! ACTTCATGGCTAGCCGAACA+GGG + chr4.4:3849903-3849922 MS.gene20161:CDS 50.0%
!! GTACTTGAAAGAGCACCCTG+AGG + chr4.4:3848505-3848524 MS.gene20161:CDS 50.0%
CTCATTAGCCGCAGTTGCTC+CGG - chr4.4:3848669-3848688 None:intergenic 55.0%
GCCGGGGTTTGCAAGAATGA+AGG - chr4.4:3848699-3848718 None:intergenic 55.0%
!! AGGAACAAGCAAGGCATCGC+CGG - chr4.4:3848717-3848736 None:intergenic 55.0%
ATTAGCCGCAGTTGCTCCGG+CGG - chr4.4:3848666-3848685 None:intergenic 60.0%
!! GAACAAGCAAGGCATCGCCG+GGG - chr4.4:3848715-3848734 None:intergenic 60.0%
!! GGAACAAGCAAGGCATCGCC+GGG - chr4.4:3848716-3848735 None:intergenic 60.0%
CATCACCGCCGGAGCAACTG+CGG + chr4.4:3848658-3848677 MS.gene20161:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3848193 3849940 3848193 ID=MS.gene20161
chr4.4 mRNA 3848193 3849940 3848193 ID=MS.gene20161.t1;Parent=MS.gene20161
chr4.4 exon 3848193 3848363 3848193 ID=MS.gene20161.t1.exon1;Parent=MS.gene20161.t1
chr4.4 CDS 3848193 3848363 3848193 ID=cds.MS.gene20161.t1;Parent=MS.gene20161.t1
chr4.4 exon 3848482 3848753 3848482 ID=MS.gene20161.t1.exon2;Parent=MS.gene20161.t1
chr4.4 CDS 3848482 3848753 3848482 ID=cds.MS.gene20161.t1;Parent=MS.gene20161.t1
chr4.4 exon 3849076 3849940 3849076 ID=MS.gene20161.t1.exon3;Parent=MS.gene20161.t1
chr4.4 CDS 3849076 3849940 3849076 ID=cds.MS.gene20161.t1;Parent=MS.gene20161.t1
Gene Sequence

>MS.gene20161

ATGGGTATTGAGATAGAACAAACATGTGTGCAGCTTTCAAACATTGCAATTTCTGACACTCATGGAGAAAACTCACCCTACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATGATGAATTAACTAACTCTTCAGGAGTTATACAAATGGGACTGGCCGAAAATCAAGTATGATTATATATATCTTGAATAAGTCTTTTTATGTCTATATATAATTAGCTCCTAATTAATTAGTTCATTAATTAATGGTTTTGGAACTAACTATATATTAATGTGTATTGTGCAGGTTTCATTTGATTTGGTAGAAAAGTACTTGAAAGAGCACCCTGAGGATTATAATGGTTTCAGAGAGAATGCATTATTTCAAGACTATCATGGACTTAAATCATTCAGAAGTGCAATGGCAAGTTTCATGGAACAAATAAGAGGTGGTAGAGCTAAATTTGATCCAGAAAGAATAGTCATCACCGCCGGAGCAACTGCGGCTAATGAGCTTTTAACCTTCATTCTTGCAAACCCCGGCGATGCCTTGCTTGTTCCTACCCCTTACTATCCAGGGTAAGAATCAAGCACCATACAATCCGGCTATGTTTGGATAAACAACTTAATTAATAAACTATTTTTAAAAATACAAGATAAAATACAATTAAACTATTTTCATCAACTATCCTCAGGAGTTATTCAAAATTAAATAGTTTAAGAACAAGTCATAAATTGTTTCCATAAACACTCTCAAGTAAAATATTTATTGATAAAAACCTTCTAGCTAGCTAGCTTCATTTGTTTTAATTTGTCATTTGAAGCTATAGCATGTAACCATGCTTGCGTAGACTATATATGATGAAATAATCTCATGTATCTTTTGCTTGTACTTTTGTAGGTTTGATAGAGACTTAAGGTGGAGAACTGGTGTAAACATAGTGCCAATTCACTGCAACAGGTCAAACAATTTTCAAATTACACCTCAAGCTTTAGAAGCAGCTTACAAAGAAGCAGAATCAATGAACATGAAAGTGAGTGGAGTACTCATCACAAACCCTTCAAACCCTTTAGGTATAACAGTTCAACGCACAGTTCTTGAAGAGATTCTTGACTTTGTGACTAAAAAGAACATCCACCTCATCTCAGACGAAATCTACTCGGGCTCGGTCTTTAATTCATCTGAGTTTGTAAGCATAGCAGAGATTCTCGAAGCTCGTCGATACAAAGATGCAGAGAGAGTTCACATTGTTTATAGTCTCTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTTGGAACAATTTATTCATACAATGATAAGGTTGTAACAACAGCGCGAAGAATGTCGAGTTTTACCATGATATCTTCACAAACACAACAGTTTTTGGCTAATATGCTATCAGACAAGGAGTTTACAGAGAAATATATTAAGATTAATAGGGAGAGATTAAAGAAGAGATATGAAATGATTATTGAAGGCTTGAAAAGTGTTGGAATTGAGTGTTTGAAAGGGAATGCAGGATTGTTTTGTTGGATGAATTTGAGTCCACTTTTGAAGGAGTCAACTAAGGAAGGTGAATTGGAACTTTGGAATGTTGTTTTGAATGAACTGAAATTGAATATATCTCCAGGGTGTTCTTGTCATTGTGATCAACCTGGTTGGTTTAGGGTCTGTTTTGCAAATATGAGTGAACAAACTTTGGATGTTGCATTGGAAAGAATACGTAACTTCATGGCTAGCCGAACAGGGACAAAGAAGATGTAA
Protein sequence

>MS.gene20161.t1

MGIEIEQTCVQLSNIAISDTHGENSPYFAGWKAYDENPYDELTNSSGVIQMGLAENQVSFDLVEKYLKEHPEDYNGFRENALFQDYHGLKSFRSAMASFMEQIRGGRAKFDPERIVITAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCNRSNNFQITPQALEAAYKEAESMNMKVSGVLITNPSNPLGITVQRTVLEEILDFVTKKNIHLISDEIYSGSVFNSSEFVSIAEILEARRYKDAERVHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTMISSQTQQFLANMLSDKEFTEKYIKINRERLKKRYEMIIEGLKSVGIECLKGNAGLFCWMNLSPLLKESTKEGELELWNVVLNELKLNISPGCSCHCDQPGWFRVCFANMSEQTLDVALERIRNFMASRTGTKKM