AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06177

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06177.t1 MTR_8g101750 95.632 435 19 0 1 435 1 435 0.0 876
MS.gene06177.t1 MTR_8g101820 89.425 435 33 2 1 435 1 422 0.0 809
MS.gene06177.t1 MTR_6g091760 81.236 437 74 2 1 429 1 437 0.0 756
MS.gene06177.t1 MTR_5g015020 58.891 433 161 6 12 428 4 435 0.0 540
MS.gene06177.t1 MTR_4g097540 57.895 437 166 5 12 431 4 439 0.0 538
MS.gene06177.t1 MTR_3g103550 56.977 430 174 3 12 430 2 431 0.0 531
MS.gene06177.t1 MTR_8g098930 56.941 425 174 2 12 427 12 436 0.0 524
MS.gene06177.t1 MTR_8g028600 55.711 429 182 2 12 433 11 438 0.0 522
MS.gene06177.t1 MTR_7g079080 56.177 429 181 1 12 433 11 439 0.0 521
MS.gene06177.t1 MTR_6g463260 44.731 427 227 2 10 428 102 527 9.00e-145 423
MS.gene06177.t1 MTR_7g079080 55.988 334 147 0 100 433 1 334 7.58e-142 410
MS.gene06177.t1 MTR_5g011400 43.735 407 219 3 24 422 84 488 1.86e-130 385
MS.gene06177.t1 MTR_2g090730 56.757 74 28 1 77 150 31 100 3.11e-21 91.7
MS.gene06177.t1 MTR_8g091280 23.174 397 262 15 46 426 97 466 1.24e-16 82.0
MS.gene06177.t1 MTR_5g033230 24.595 309 212 12 83 373 103 408 2.32e-16 81.3
MS.gene06177.t1 MTR_5g033230 24.595 309 212 12 83 373 103 408 2.97e-16 81.3
MS.gene06177.t1 MTR_4g011820 23.101 316 211 11 115 423 92 382 1.77e-15 78.2
MS.gene06177.t1 MTR_2g090615 26.087 276 167 9 88 350 86 337 2.82e-14 74.7
MS.gene06177.t1 MTR_8g023140 22.372 371 260 14 78 425 149 514 3.50e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06177.t1 AT4G26200 75.524 429 96 3 10 429 16 444 0.0 663
MS.gene06177.t1 AT4G37770 59.353 433 166 2 12 434 4 436 0.0 554
MS.gene06177.t1 AT4G08040 57.907 430 174 1 12 434 2 431 0.0 547
MS.gene06177.t1 AT5G65800 58.178 428 169 3 11 428 3 430 0.0 533
MS.gene06177.t1 AT3G49700 58.275 429 167 3 11 428 3 430 0.0 529
MS.gene06177.t1 AT2G22810 55.963 436 181 3 10 434 2 437 0.0 526
MS.gene06177.t1 AT3G61510 56.398 422 174 2 12 426 11 429 0.0 525
MS.gene06177.t1 AT4G11280 56.148 431 182 1 4 427 7 437 0.0 519
MS.gene06177.t1 AT1G01480 51.945 437 200 3 1 427 1 437 1.13e-172 493
MS.gene06177.t1 AT5G51690 44.028 427 230 3 10 428 67 492 1.91e-138 406
MS.gene06177.t1 AT1G01480 52.870 331 153 2 100 427 1 331 1.93e-134 392
MS.gene06177.t1 AT1G62960 43.210 405 227 3 22 423 143 547 2.18e-127 380
MS.gene06177.t1 AT5G51690 46.839 348 184 1 81 428 23 369 5.71e-124 365
MS.gene06177.t1 AT1G23310 25.632 277 188 9 83 344 103 376 7.34e-18 85.9
MS.gene06177.t1 AT4G23600 25.627 359 230 13 88 433 78 412 8.40e-18 85.5
MS.gene06177.t1 AT1G23310 25.632 277 188 9 83 344 103 376 9.64e-18 85.9
MS.gene06177.t1 AT5G36160 24.022 358 231 13 83 425 80 411 1.75e-17 84.7
MS.gene06177.t1 AT1G72330 22.955 379 264 14 73 428 160 533 2.06e-17 84.7
MS.gene06177.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.02e-17 83.6
MS.gene06177.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.02e-17 83.6
MS.gene06177.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.02e-17 83.6
MS.gene06177.t1 AT1G70580 25.271 277 189 10 83 344 103 376 5.02e-17 83.6
MS.gene06177.t1 AT1G72330 23.970 267 189 8 73 328 160 423 1.17e-16 82.0
MS.gene06177.t1 AT1G72330 22.194 392 264 14 73 428 160 546 1.20e-15 79.3
MS.gene06177.t1 AT1G80360 23.485 396 250 15 41 424 28 382 2.99e-15 77.4
MS.gene06177.t1 AT1G80360 23.485 396 250 15 41 424 28 382 2.99e-15 77.4
MS.gene06177.t1 AT1G80360 23.485 396 250 15 41 424 28 382 2.99e-15 77.4
MS.gene06177.t1 AT1G80360 23.485 396 250 15 41 424 28 382 2.99e-15 77.4
MS.gene06177.t1 AT2G20610 24.507 355 223 14 88 425 109 435 3.02e-15 77.8
MS.gene06177.t1 AT1G17290 22.483 298 211 11 78 359 168 461 7.64e-15 77.0
MS.gene06177.t1 AT4G23600 25.455 330 211 12 117 433 1 308 1.16e-14 75.1
MS.gene06177.t1 AT1G77670 24.340 341 233 11 87 424 116 434 1.26e-14 75.9
MS.gene06177.t1 AT4G23600 25.472 318 203 11 88 392 78 374 2.92e-14 74.3
MS.gene06177.t1 AT2G20610 25.461 271 167 9 88 345 109 357 3.79e-14 74.3
MS.gene06177.t1 AT4G28410 26.619 278 163 11 88 350 111 362 1.00e-11 67.0
MS.gene06177.t1 AT4G28410 26.642 274 168 10 88 350 111 362 1.28e-11 66.6
MS.gene06177.t1 AT5G53970 23.416 363 233 14 88 433 76 410 3.74e-11 65.1
MS.gene06177.t1 AT4G23590 23.851 348 234 12 88 425 78 404 4.33e-11 65.1

Find 88 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATATATTAAGATTAAT+AGG 0.143027 4.2:+3332919 MS.gene06177:CDS
GCGGTGATGACTATTCTTTC+TGG 0.188225 4.2:-3331969 None:intergenic
GTGATCAACCTGGTTGGTTT+AGG 0.197998 4.2:+3333147 MS.gene06177:CDS
TGATCAACCTGGTTGGTTTA+GGG 0.271865 4.2:+3333148 MS.gene06177:CDS
AACTTCATGGCTAGCCGAAC+AGG 0.317741 4.2:+3333227 MS.gene06177:CDS
ACAAACCCTTCAAACCCTTT+AGG 0.351926 4.2:+3332552 MS.gene06177:CDS
GTCAACTAAGGAAGGTGAAT+TGG 0.354677 4.2:+3333061 MS.gene06177:CDS
GATGAATTAACTAACTCTTC+AGG 0.373544 4.2:+3331635 MS.gene06177:CDS
TGTGCAGGTTTCATTTGATT+TGG 0.377676 4.2:+3331800 MS.gene06177:intron
GAAAACTCACCCTACTTTGC+TGG 0.379915 4.2:+3331584 MS.gene06177:CDS
GAGTTAGTTAATTCATCATA+AGG 0.381161 4.2:-3331629 None:intergenic
GATTCTTACCCTGGATAGTA+AGG 0.392460 4.2:-3332065 None:intergenic
TATAGTCTCTCTAAAGATCT+TGG 0.393148 4.2:+3332753 MS.gene06177:CDS
CTCATTAGCCGCAGTTGCTC+CGG 0.395906 4.2:-3331991 None:intergenic
ACATCTTCTTTGTCCCTGTT+CGG 0.397573 4.2:-3333241 None:intergenic
GAAATATATTAAGATTAATA+GGG 0.399795 4.2:+3332920 MS.gene06177:CDS
AGGAACAAGCAAGGCATCGC+CGG 0.403862 4.2:-3332039 None:intergenic
CTGAAACCATTATAATCCTC+AGG 0.404711 4.2:-3331846 None:intergenic
AAGTGCAATGGCAAGTTTCA+TGG 0.405411 4.2:+3331914 MS.gene06177:CDS
GAGCACCCTGAGGATTATAA+TGG 0.406179 4.2:+3331840 MS.gene06177:CDS
GTTCCTACCCCTTACTATCC+AGG 0.407257 4.2:+3332056 MS.gene06177:CDS
GGAAAGAATACGTAACTTCA+TGG 0.412992 4.2:+3333214 MS.gene06177:CDS
TTTGACCTGTTGCAGTGAAT+TGG 0.417511 4.2:-3332444 None:intergenic
CGTTGAACTGTTATACCTAA+AGG 0.428028 4.2:-3332567 None:intergenic
GTTGAACTGTTATACCTAAA+GGG 0.430255 4.2:-3332566 None:intergenic
GTTATACCTAAAGGGTTTGA+AGG 0.431593 4.2:-3332558 None:intergenic
AATTTATTCATACAATGATA+AGG 0.432216 4.2:+3332800 MS.gene06177:CDS
AGATATGAAATGATTATTGA+AGG 0.438836 4.2:+3332957 MS.gene06177:CDS
TAGTGCCAATTCACTGCAAC+AGG 0.441325 4.2:+3332439 MS.gene06177:CDS
CTGAAATTGAATATATCTCC+AGG 0.445529 4.2:+3333110 MS.gene06177:CDS
GTAGGTTTGATAGAGACTTA+AGG 0.446910 4.2:+3332397 MS.gene06177:intron
GGAACAAGCAAGGCATCGCC+GGG 0.449445 4.2:-3332038 None:intergenic
GCATTATTTCAAGACTATCA+TGG 0.449699 4.2:+3331876 MS.gene06177:CDS
ATTGAAGGCTTGAAAAGTGT+TGG 0.452654 4.2:+3332972 MS.gene06177:CDS
TTAGTTGACTCCTTCAAAAG+TGG 0.458012 4.2:-3333047 None:intergenic
GAGTGTTTGAAAGGGAATGC+AGG 0.465675 4.2:+3332999 MS.gene06177:CDS
AGTTAGTTAATTCATCATAA+GGG 0.468946 4.2:-3331628 None:intergenic
AGGAAGGTGAATTGGAACTT+TGG 0.476087 4.2:+3333069 MS.gene06177:CDS
ACAAACTTTGGATGTTGCAT+TGG 0.480931 4.2:+3333193 MS.gene06177:CDS
GGTCTACCAGGTTTCAGAGT+TGG 0.483669 4.2:+3332774 MS.gene06177:CDS
AAATATGAGTGAACAAACTT+TGG 0.486245 4.2:+3333181 MS.gene06177:CDS
CTCTTCAGGAGTTATACAAA+TGG 0.488431 4.2:+3331649 MS.gene06177:CDS
AGAGACTTAAGGTGGAGAAC+TGG 0.493331 4.2:+3332408 MS.gene06177:CDS
CATCTCAGACGAAATCTACT+CGG 0.501179 4.2:+3332641 MS.gene06177:CDS
GGCTTTCCATCCAGCAAAGT+AGG 0.501359 4.2:-3331594 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.510586 4.2:+3332762 MS.gene06177:CDS
ACAGGGACAAAGAAGATGTA+AGG 0.520984 4.2:+3333245 MS.gene06177:CDS
GCCGGGGTTTGCAAGAATGA+AGG 0.526652 4.2:-3332021 None:intergenic
ACCTTCATTCTTGCAAACCC+CGG 0.529279 4.2:+3332020 MS.gene06177:CDS
ATTGCAATTTCTGACACTCA+TGG 0.531513 4.2:+3331560 MS.gene06177:CDS
TCTTCAGGAGTTATACAAAT+GGG 0.535454 4.2:+3331650 MS.gene06177:CDS
GTCATTGTGATCAACCTGGT+TGG 0.538197 4.2:+3333141 MS.gene06177:CDS
TTCCTACCCCTTACTATCCA+GGG 0.542899 4.2:+3332057 MS.gene06177:CDS
TTATACCTAAAGGGTTTGAA+GGG 0.548536 4.2:-3332557 None:intergenic
ATTCTTACCCTGGATAGTAA+GGG 0.548613 4.2:-3332064 None:intergenic
TAAGCTGCTTCTAAAGCTTG+AGG 0.550801 4.2:-3332483 None:intergenic
AGACGAAATCTACTCGGGCT+CGG 0.552466 4.2:+3332647 MS.gene06177:CDS
TTGTGTTTGTGAAGATATCA+AGG 0.553420 4.2:-3332856 None:intergenic
GGTTTGATAGAGACTTAAGG+TGG 0.553899 4.2:+3332400 MS.gene06177:intron
TTGAAGGAGTCAACTAAGGA+AGG 0.555608 4.2:+3333053 MS.gene06177:CDS
AGATTTCGTCTGAGATGAGG+TGG 0.558059 4.2:-3332635 None:intergenic
GTTGGAATTGAGTGTTTGAA+AGG 0.569169 4.2:+3332990 MS.gene06177:CDS
ACTCACCCTACTTTGCTGGA+TGG 0.572647 4.2:+3331588 MS.gene06177:CDS
GGCTAATATGCTATCAGACA+AGG 0.573142 4.2:+3332887 MS.gene06177:CDS
TTGGAATTGAGTGTTTGAAA+GGG 0.574213 4.2:+3332991 MS.gene06177:CDS
AGTTTCATGGAACAAATAAG+AGG 0.575774 4.2:+3331927 MS.gene06177:CDS
GCTTTCCATCCAGCAAAGTA+GGG 0.580141 4.2:-3331593 None:intergenic
AGGAGTTATACAAATGGGAC+TGG 0.580638 4.2:+3331655 MS.gene06177:CDS
TAAATCATTCAGAAGTGCAA+TGG 0.583118 4.2:+3331902 MS.gene06177:CDS
TCTTGTCATTGTGATCAACC+TGG 0.587957 4.2:+3333137 MS.gene06177:CDS
ATTAGCCGCAGTTGCTCCGG+CGG 0.589538 4.2:-3331988 None:intergenic
CATCACCGCCGGAGCAACTG+CGG 0.595084 4.2:+3331983 MS.gene06177:CDS
ATCTCAGACGAAATCTACTC+GGG 0.596514 4.2:+3332642 MS.gene06177:CDS
TTCTTACCCTGGATAGTAAG+GGG 0.604583 4.2:-3332063 None:intergenic
TCAATGAACATGAAAGTGAG+TGG 0.607414 4.2:+3332519 MS.gene06177:CDS
ATTGTTCCAACTCTGAAACC+TGG 0.608322 4.2:-3332780 None:intergenic
TGAAACCATTATAATCCTCA+GGG 0.627551 4.2:-3331845 None:intergenic
ACTTCATGGCTAGCCGAACA+GGG 0.629105 4.2:+3333228 MS.gene06177:CDS
GAAAGAATAGTCATCACCGC+CGG 0.631099 4.2:+3331972 MS.gene06177:CDS
AGTAGATTTCGTCTGAGATG+AGG 0.631875 4.2:-3332638 None:intergenic
TACCCTGGATAGTAAGGGGT+AGG 0.633960 4.2:-3332059 None:intergenic
TGAAATTGAATATATCTCCA+GGG 0.640695 4.2:+3333111 MS.gene06177:CDS
TTCATGGAACAAATAAGAGG+TGG 0.647531 4.2:+3331930 MS.gene06177:CDS
GTAAGGGGTAGGAACAAGCA+AGG 0.672268 4.2:-3332048 None:intergenic
AAACAGACCCTAAACCAACC+AGG 0.685588 4.2:-3333155 None:intergenic
TCACAATGACAAGAACACCC+TGG 0.704106 4.2:-3333128 None:intergenic
GAACAAGCAAGGCATCGCCG+GGG 0.751656 4.2:-3332037 None:intergenic
GTACTTGAAAGAGCACCCTG+AGG 0.755804 4.2:+3331830 MS.gene06177:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGAACTAATTAATT+AGG - chr4.2:3331745-3331764 None:intergenic 10.0%
!! AGAAATATATTAAGATTAAT+AGG + chr4.2:3332919-3332938 MS.gene06177:CDS 10.0%
!! GAAATATATTAAGATTAATA+GGG + chr4.2:3332920-3332939 MS.gene06177:CDS 10.0%
!! TTAATTAGTTCATTAATTAA+TGG + chr4.2:3331747-3331766 MS.gene06177:intron 10.0%
!! AATTTATTCATACAATGATA+AGG + chr4.2:3332800-3332819 MS.gene06177:CDS 15.0%
!!! ATATAATCATACTTGATTTT+CGG - chr4.2:3331681-3331700 None:intergenic 15.0%
!! AGATATGAAATGATTATTGA+AGG + chr4.2:3332957-3332976 MS.gene06177:CDS 20.0%
!! AGTTAGTTAATTCATCATAA+GGG - chr4.2:3331631-3331650 None:intergenic 20.0%
!!! AGTTCATTAATTAATGGTTT+TGG + chr4.2:3331753-3331772 MS.gene06177:intron 20.0%
! AAATATGAGTGAACAAACTT+TGG + chr4.2:3333181-3333200 MS.gene06177:CDS 25.0%
! GAGTTAGTTAATTCATCATA+AGG - chr4.2:3331632-3331651 None:intergenic 25.0%
! TATATTAATGTGTATTGTGC+AGG + chr4.2:3331785-3331804 MS.gene06177:intron 25.0%
! TGAAATTGAATATATCTCCA+GGG + chr4.2:3333111-3333130 MS.gene06177:CDS 25.0%
!! TTAATTTTGAATAACTCCTG+AGG - chr4.2:3332192-3332211 None:intergenic 25.0%
!!! ATTTTACTTGAGAGTGTTTA+TGG - chr4.2:3332244-3332263 None:intergenic 25.0%
AGTTTCATGGAACAAATAAG+AGG + chr4.2:3331927-3331946 MS.gene06177:CDS 30.0%
CTGAAATTGAATATATCTCC+AGG + chr4.2:3333110-3333129 MS.gene06177:CDS 30.0%
GATGAATTAACTAACTCTTC+AGG + chr4.2:3331635-3331654 MS.gene06177:CDS 30.0%
GCATTATTTCAAGACTATCA+TGG + chr4.2:3331876-3331895 MS.gene06177:CDS 30.0%
GTTGAACTGTTATACCTAAA+GGG - chr4.2:3332569-3332588 None:intergenic 30.0%
TCTTCAGGAGTTATACAAAT+GGG + chr4.2:3331650-3331669 MS.gene06177:CDS 30.0%
TGAAACCATTATAATCCTCA+GGG - chr4.2:3331848-3331867 None:intergenic 30.0%
TTATACCTAAAGGGTTTGAA+GGG - chr4.2:3332560-3332579 None:intergenic 30.0%
TTGGAATTGAGTGTTTGAAA+GGG + chr4.2:3332991-3333010 MS.gene06177:CDS 30.0%
TTGTGTTTGTGAAGATATCA+AGG - chr4.2:3332859-3332878 None:intergenic 30.0%
! ATCATAAGGGTTTTCATCAT+AGG - chr4.2:3331618-3331637 None:intergenic 30.0%
! TAAATCATTCAGAAGTGCAA+TGG + chr4.2:3331902-3331921 MS.gene06177:CDS 30.0%
! TATAGTCTCTCTAAAGATCT+TGG + chr4.2:3332753-3332772 MS.gene06177:CDS 30.0%
! TATTTTCATCAACTATCCTC+AGG + chr4.2:3332173-3332192 MS.gene06177:intron 30.0%
! TCTTTTGCTTGTACTTTTGT+AGG + chr4.2:3332379-3332398 MS.gene06177:intron 30.0%
! TTCACAAACACAACAGTTTT+TGG + chr4.2:3332866-3332885 MS.gene06177:CDS 30.0%
AATGAAGCTAGCTAGATAGA+AGG - chr4.2:3332282-3332301 None:intergenic 35.0%
ACAAACTTTGGATGTTGCAT+TGG + chr4.2:3333193-3333212 MS.gene06177:CDS 35.0%
ATATATAGTCTACGCAAGCA+TGG - chr4.2:3332340-3332359 None:intergenic 35.0%
ATTCTTACCCTGGATAGTAA+GGG - chr4.2:3332067-3332086 None:intergenic 35.0%
ATTGCAATTTCTGACACTCA+TGG + chr4.2:3331560-3331579 MS.gene06177:CDS 35.0%
CGTTGAACTGTTATACCTAA+AGG - chr4.2:3332570-3332589 None:intergenic 35.0%
CTCTTCAGGAGTTATACAAA+TGG + chr4.2:3331649-3331668 MS.gene06177:CDS 35.0%
CTGAAACCATTATAATCCTC+AGG - chr4.2:3331849-3331868 None:intergenic 35.0%
GGAAAGAATACGTAACTTCA+TGG + chr4.2:3333214-3333233 MS.gene06177:CDS 35.0%
GTAGGTTTGATAGAGACTTA+AGG + chr4.2:3332397-3332416 MS.gene06177:intron 35.0%
GTTATACCTAAAGGGTTTGA+AGG - chr4.2:3332561-3332580 None:intergenic 35.0%
GTTGGAATTGAGTGTTTGAA+AGG + chr4.2:3332990-3333009 MS.gene06177:CDS 35.0%
GTTGTTTATCCAAACATAGC+CGG - chr4.2:3332105-3332124 None:intergenic 35.0%
TCAATGAACATGAAAGTGAG+TGG + chr4.2:3332519-3332538 MS.gene06177:CDS 35.0%
TGTGCAGGTTTCATTTGATT+TGG + chr4.2:3331800-3331819 MS.gene06177:intron 35.0%
TTCATGGAACAAATAAGAGG+TGG + chr4.2:3331930-3331949 MS.gene06177:CDS 35.0%
! ATTGAAGGCTTGAAAAGTGT+TGG + chr4.2:3332972-3332991 MS.gene06177:CDS 35.0%
! TTAGTTGACTCCTTCAAAAG+TGG - chr4.2:3333050-3333069 None:intergenic 35.0%
!! ACTTTTGAAGGAGTCAACTA+AGG + chr4.2:3333049-3333068 MS.gene06177:CDS 35.0%
!! GAATTTGAGTCCACTTTTGA+AGG + chr4.2:3333037-3333056 MS.gene06177:CDS 35.0%
AAGTGCAATGGCAAGTTTCA+TGG + chr4.2:3331914-3331933 MS.gene06177:CDS 40.0%
ACAAACCCTTCAAACCCTTT+AGG + chr4.2:3332552-3332571 MS.gene06177:CDS 40.0%
ACATCTTCTTTGTCCCTGTT+CGG - chr4.2:3333244-3333263 None:intergenic 40.0%
AGGAAGGTGAATTGGAACTT+TGG + chr4.2:3333069-3333088 MS.gene06177:CDS 40.0%
AGGAGTTATACAAATGGGAC+TGG + chr4.2:3331655-3331674 MS.gene06177:CDS 40.0%
ATCTCAGACGAAATCTACTC+GGG + chr4.2:3332642-3332661 MS.gene06177:CDS 40.0%
CATCTCAGACGAAATCTACT+CGG + chr4.2:3332641-3332660 MS.gene06177:CDS 40.0%
GAATCAAGCACCATACAATC+CGG + chr4.2:3332083-3332102 MS.gene06177:intron 40.0%
GATTCTTACCCTGGATAGTA+AGG - chr4.2:3332068-3332087 None:intergenic 40.0%
GGCTAATATGCTATCAGACA+AGG + chr4.2:3332887-3332906 MS.gene06177:CDS 40.0%
GGTTTGATAGAGACTTAAGG+TGG + chr4.2:3332400-3332419 MS.gene06177:intron 40.0%
GTCAACTAAGGAAGGTGAAT+TGG + chr4.2:3333061-3333080 MS.gene06177:CDS 40.0%
TCTAAAGATCTTGGTCTACC+AGG + chr4.2:3332762-3332781 MS.gene06177:CDS 40.0%
TCTTGTCATTGTGATCAACC+TGG + chr4.2:3333137-3333156 MS.gene06177:CDS 40.0%
TTCTTACCCTGGATAGTAAG+GGG - chr4.2:3332066-3332085 None:intergenic 40.0%
TTGAAGGAGTCAACTAAGGA+AGG + chr4.2:3333053-3333072 MS.gene06177:CDS 40.0%
TTTGACCTGTTGCAGTGAAT+TGG - chr4.2:3332447-3332466 None:intergenic 40.0%
! AGTAGATTTCGTCTGAGATG+AGG - chr4.2:3332641-3332660 None:intergenic 40.0%
!! ATTGTTCCAACTCTGAAACC+TGG - chr4.2:3332783-3332802 None:intergenic 40.0%
!! GGAATGCAGGATTGTTTTGT+TGG + chr4.2:3333012-3333031 MS.gene06177:CDS 40.0%
!! TAAGCTGCTTCTAAAGCTTG+AGG - chr4.2:3332486-3332505 None:intergenic 40.0%
!! TGATCAACCTGGTTGGTTTA+GGG + chr4.2:3333148-3333167 MS.gene06177:CDS 40.0%
AAACAGACCCTAAACCAACC+AGG - chr4.2:3333158-3333177 None:intergenic 45.0%
ACCTTCATTCTTGCAAACCC+CGG + chr4.2:3332020-3332039 MS.gene06177:CDS 45.0%
AGAGACTTAAGGTGGAGAAC+TGG + chr4.2:3332408-3332427 MS.gene06177:CDS 45.0%
ATGGTGCTTGATTCTTACCC+TGG - chr4.2:3332077-3332096 None:intergenic 45.0%
CCAAACATAGCCGGATTGTA+TGG - chr4.2:3332096-3332115 None:intergenic 45.0%
CCATACAATCCGGCTATGTT+TGG + chr4.2:3332093-3332112 MS.gene06177:intron 45.0%
GAAAACTCACCCTACTTTGC+TGG + chr4.2:3331584-3331603 MS.gene06177:CDS 45.0%
GAAAGAATAGTCATCACCGC+CGG + chr4.2:3331972-3331991 MS.gene06177:CDS 45.0%
GAGCACCCTGAGGATTATAA+TGG + chr4.2:3331840-3331859 MS.gene06177:CDS 45.0%
GAGTGTTTGAAAGGGAATGC+AGG + chr4.2:3332999-3333018 MS.gene06177:CDS 45.0%
GCGGTGATGACTATTCTTTC+TGG - chr4.2:3331972-3331991 None:intergenic 45.0%
GCTTTCCATCCAGCAAAGTA+GGG - chr4.2:3331596-3331615 None:intergenic 45.0%
GTCATTGTGATCAACCTGGT+TGG + chr4.2:3333141-3333160 MS.gene06177:CDS 45.0%
TAGTGCCAATTCACTGCAAC+AGG + chr4.2:3332439-3332458 MS.gene06177:CDS 45.0%
TCACAATGACAAGAACACCC+TGG - chr4.2:3333131-3333150 None:intergenic 45.0%
TTCCTACCCCTTACTATCCA+GGG + chr4.2:3332057-3332076 MS.gene06177:CDS 45.0%
! AGATTTCGTCTGAGATGAGG+TGG - chr4.2:3332638-3332657 None:intergenic 45.0%
!! GTGATCAACCTGGTTGGTTT+AGG + chr4.2:3333147-3333166 MS.gene06177:CDS 45.0%
ACTCACCCTACTTTGCTGGA+TGG + chr4.2:3331588-3331607 MS.gene06177:CDS 50.0%
AGACGAAATCTACTCGGGCT+CGG + chr4.2:3332647-3332666 MS.gene06177:CDS 50.0%
GGCTTTCCATCCAGCAAAGT+AGG - chr4.2:3331597-3331616 None:intergenic 50.0%
GGTCTACCAGGTTTCAGAGT+TGG + chr4.2:3332774-3332793 MS.gene06177:CDS 50.0%
GTAAGGGGTAGGAACAAGCA+AGG - chr4.2:3332051-3332070 None:intergenic 50.0%
GTTCCTACCCCTTACTATCC+AGG + chr4.2:3332056-3332075 MS.gene06177:CDS 50.0%
TACCCTGGATAGTAAGGGGT+AGG - chr4.2:3332062-3332081 None:intergenic 50.0%
! AACTTCATGGCTAGCCGAAC+AGG + chr4.2:3333227-3333246 MS.gene06177:CDS 50.0%
! ACTTCATGGCTAGCCGAACA+GGG + chr4.2:3333228-3333247 MS.gene06177:CDS 50.0%
!! GTACTTGAAAGAGCACCCTG+AGG + chr4.2:3331830-3331849 MS.gene06177:CDS 50.0%
CTCATTAGCCGCAGTTGCTC+CGG - chr4.2:3331994-3332013 None:intergenic 55.0%
GCCGGGGTTTGCAAGAATGA+AGG - chr4.2:3332024-3332043 None:intergenic 55.0%
!! AGGAACAAGCAAGGCATCGC+CGG - chr4.2:3332042-3332061 None:intergenic 55.0%
ATTAGCCGCAGTTGCTCCGG+CGG - chr4.2:3331991-3332010 None:intergenic 60.0%
!! GAACAAGCAAGGCATCGCCG+GGG - chr4.2:3332040-3332059 None:intergenic 60.0%
!! GGAACAAGCAAGGCATCGCC+GGG - chr4.2:3332041-3332060 None:intergenic 60.0%
CATCACCGCCGGAGCAACTG+CGG + chr4.2:3331983-3332002 MS.gene06177:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 3331518 3333265 3331518 ID=MS.gene06177
chr4.2 mRNA 3331518 3333265 3331518 ID=MS.gene06177.t1;Parent=MS.gene06177
chr4.2 exon 3331518 3331688 3331518 ID=MS.gene06177.t1.exon1;Parent=MS.gene06177.t1
chr4.2 CDS 3331518 3331688 3331518 ID=cds.MS.gene06177.t1;Parent=MS.gene06177.t1
chr4.2 exon 3331807 3332078 3331807 ID=MS.gene06177.t1.exon2;Parent=MS.gene06177.t1
chr4.2 CDS 3331807 3332078 3331807 ID=cds.MS.gene06177.t1;Parent=MS.gene06177.t1
chr4.2 exon 3332401 3333265 3332401 ID=MS.gene06177.t1.exon3;Parent=MS.gene06177.t1
chr4.2 CDS 3332401 3333265 3332401 ID=cds.MS.gene06177.t1;Parent=MS.gene06177.t1
Gene Sequence

>MS.gene06177

ATGGGTATTGAGATAGAACAAACATGTGTGCAGCTTTCAAACATTGCAATTTCTGACACTCATGGAGAAAACTCACCCTACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATGATGAATTAACTAACTCTTCAGGAGTTATACAAATGGGACTGGCCGAAAATCAAGTATGATTATATATATCTTGAATAAGTCTTTTTATGTCTATATATAATTAGCTCCTAATTAATTAGTTCATTAATTAATGGTTTTGGAACTAACTATATATTAATGTGTATTGTGCAGGTTTCATTTGATTTGGTAGAAAAGTACTTGAAAGAGCACCCTGAGGATTATAATGGTTTCAGAGAGAATGCATTATTTCAAGACTATCATGGACTTAAATCATTCAGAAGTGCAATGGCAAGTTTCATGGAACAAATAAGAGGTGGTAGAGCTAAATTTGATCCAGAAAGAATAGTCATCACCGCCGGAGCAACTGCGGCTAATGAGCTTTTAACCTTCATTCTTGCAAACCCCGGCGATGCCTTGCTTGTTCCTACCCCTTACTATCCAGGGTAAGAATCAAGCACCATACAATCCGGCTATGTTTGGATAAACAACTTAATTAATAAACTATTTTTAAAAATACAAGATAAAATACAATTAAACTATTTTCATCAACTATCCTCAGGAGTTATTCAAAATTAAATAGTTTAAGAACAAGTCATAAATTGTTTCCATAAACACTCTCAAGTAAAATATTTATTGATAAAAACCTTCTATCTAGCTAGCTTCATTTGTTTTAATTTGTCATTTGAAGCTATAGCATGTAACCATGCTTGCGTAGACTATATATGATGAAATAATCTCATGTATCTTTTGCTTGTACTTTTGTAGGTTTGATAGAGACTTAAGGTGGAGAACTGGTGTAAACATAGTGCCAATTCACTGCAACAGGTCAAACAATTTTCAAATTACACCTCAAGCTTTAGAAGCAGCTTACAAAGAAGCAGAATCAATGAACATGAAAGTGAGTGGAGTACTCATCACAAACCCTTCAAACCCTTTAGGTATAACAGTTCAACGCACAGTTCTTGAAGAGATTCTTGACTTTGTGACTAAAAAGAACATCCACCTCATCTCAGACGAAATCTACTCGGGCTCGGTCTTTAATTCATCTGAGTTTGTAAGCATAGCAGAGATTCTCGAAGCTCGTCGATACAAAGATGCAGAGAGAGTTCACATTGTTTATAGTCTCTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTTGGAACAATTTATTCATACAATGATAAGGTTGTAACAACAGCGCGAAGAATGTCGAGTTTTACCTTGATATCTTCACAAACACAACAGTTTTTGGCTAATATGCTATCAGACAAGGAGTTTACAGAGAAATATATTAAGATTAATAGGGAGAGATTAAAGAAGAGATATGAAATGATTATTGAAGGCTTGAAAAGTGTTGGAATTGAGTGTTTGAAAGGGAATGCAGGATTGTTTTGTTGGATGAATTTGAGTCCACTTTTGAAGGAGTCAACTAAGGAAGGTGAATTGGAACTTTGGAATGTTGTTTTGAATGAACTGAAATTGAATATATCTCCAGGGTGTTCTTGTCATTGTGATCAACCTGGTTGGTTTAGGGTCTGTTTTGCAAATATGAGTGAACAAACTTTGGATGTTGCATTGGAAAGAATACGTAACTTCATGGCTAGCCGAACAGGGACAAAGAAGATGTAA
Protein sequence

>MS.gene06177.t1

MGIEIEQTCVQLSNIAISDTHGENSPYFAGWKAYDENPYDELTNSSGVIQMGLAENQVSFDLVEKYLKEHPEDYNGFRENALFQDYHGLKSFRSAMASFMEQIRGGRAKFDPERIVITAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCNRSNNFQITPQALEAAYKEAESMNMKVSGVLITNPSNPLGITVQRTVLEEILDFVTKKNIHLISDEIYSGSVFNSSEFVSIAEILEARRYKDAERVHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQQFLANMLSDKEFTEKYIKINRERLKKRYEMIIEGLKSVGIECLKGNAGLFCWMNLSPLLKESTKEGELELWNVVLNELKLNISPGCSCHCDQPGWFRVCFANMSEQTLDVALERIRNFMASRTGTKKM