AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66343

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66343.t1 MTR_6g091760 98.874 444 5 0 1 444 1 444 0.0 920
MS.gene66343.t1 MTR_8g101750 81.922 437 71 2 1 437 1 429 0.0 758
MS.gene66343.t1 MTR_8g101820 76.201 437 83 3 1 437 1 416 0.0 698
MS.gene66343.t1 MTR_5g015020 61.111 432 159 5 14 436 4 435 0.0 558
MS.gene66343.t1 MTR_4g097540 60.325 431 161 4 14 434 4 434 0.0 552
MS.gene66343.t1 MTR_3g103550 58.645 428 172 2 14 436 2 429 0.0 549
MS.gene66343.t1 MTR_8g028600 56.308 428 185 2 14 440 11 437 0.0 526
MS.gene66343.t1 MTR_8g098930 55.530 443 190 5 1 438 1 441 0.0 521
MS.gene66343.t1 MTR_7g079080 56.279 430 187 1 14 442 11 440 0.0 520
MS.gene66343.t1 MTR_6g463260 44.213 432 238 2 12 441 102 532 4.77e-146 427
MS.gene66343.t1 MTR_7g079080 55.522 335 149 0 108 442 1 335 9.61e-140 405
MS.gene66343.t1 MTR_5g011400 44.010 409 221 3 26 430 84 488 1.60e-131 389
MS.gene66343.t1 MTR_2g090730 57.692 78 29 1 81 158 27 100 4.90e-22 94.0
MS.gene66343.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 8.24e-18 85.9
MS.gene66343.t1 MTR_8g023140 23.181 371 257 14 86 433 149 514 8.31e-18 86.3
MS.gene66343.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 9.01e-18 85.9
MS.gene66343.t1 MTR_4g011820 22.917 336 222 12 105 431 75 382 1.40e-14 75.5
MS.gene66343.t1 MTR_8g061360 21.168 411 276 15 46 440 38 416 1.34e-11 66.6
MS.gene66343.t1 MTR_7g106830 19.637 331 243 10 114 436 151 466 6.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66343.t1 AT4G26200 75.638 431 102 3 12 439 16 446 0.0 665
MS.gene66343.t1 AT4G37770 60.563 426 164 2 14 435 4 429 0.0 557
MS.gene66343.t1 AT4G08040 60.798 426 166 1 14 438 2 427 0.0 554
MS.gene66343.t1 AT5G65800 58.837 430 173 2 13 438 3 432 0.0 545
MS.gene66343.t1 AT3G49700 58.333 432 176 2 13 440 3 434 0.0 542
MS.gene66343.t1 AT2G22810 58.333 432 175 3 12 438 2 433 0.0 538
MS.gene66343.t1 AT3G61510 58.531 422 171 2 14 434 11 429 0.0 536
MS.gene66343.t1 AT4G11280 58.670 421 173 1 14 433 15 435 0.0 530
MS.gene66343.t1 AT1G01480 54.524 431 192 3 14 440 12 442 2.17e-178 508
MS.gene66343.t1 AT1G01480 53.571 336 153 2 108 440 1 336 1.44e-136 397
MS.gene66343.t1 AT5G51690 43.396 424 237 2 12 433 67 489 2.31e-136 401
MS.gene66343.t1 AT1G62960 42.787 409 229 3 24 431 143 547 1.58e-127 380
MS.gene66343.t1 AT5G51690 46.064 343 184 1 89 431 23 364 3.86e-121 358
MS.gene66343.t1 AT1G72330 24.865 370 252 14 86 433 165 530 6.38e-20 92.8
MS.gene66343.t1 AT1G23310 26.259 278 185 10 91 352 103 376 8.34e-20 91.7
MS.gene66343.t1 AT1G23310 26.259 278 185 10 91 352 103 376 1.14e-19 91.7
MS.gene66343.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66343.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66343.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66343.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66343.t1 AT1G72330 24.021 383 252 14 86 433 165 543 4.23e-18 87.0
MS.gene66343.t1 AT1G72330 25.573 262 181 8 86 336 165 423 1.10e-17 85.5
MS.gene66343.t1 AT1G17290 23.826 298 207 11 86 367 168 461 3.02e-17 84.3
MS.gene66343.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66343.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66343.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66343.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66343.t1 AT2G24850 24.000 350 232 14 96 433 92 419 1.48e-15 79.0
MS.gene66343.t1 AT1G77670 24.458 323 214 11 121 437 141 439 1.63e-14 75.5
MS.gene66343.t1 AT4G23600 23.056 360 236 14 96 440 78 411 6.37e-14 73.9
MS.gene66343.t1 AT5G36160 22.096 353 234 13 96 433 85 411 1.36e-13 72.8
MS.gene66343.t1 AT2G20610 22.284 359 240 14 96 440 109 442 2.40e-12 68.9
MS.gene66343.t1 AT4G23600 23.263 331 215 13 125 440 1 307 9.10e-12 66.2
MS.gene66343.t1 AT2G20610 23.810 273 177 10 96 358 109 360 1.01e-11 67.0
MS.gene66343.t1 AT4G23600 23.264 288 188 10 96 370 78 345 3.11e-11 65.1

Find 80 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCGAACCCGGTTGGTTT+AGG 0.229798 6.4:-63130121 MS.gene66343:CDS
TTCCGAACCCGGTTGGTTTA+GGG 0.281999 6.4:-63130120 MS.gene66343:CDS
TTGTGTTTGTGAAGATATTA+AGG 0.307747 6.4:+63130412 None:intergenic
AGGTAAAACTCGACATTCTT+CGG 0.310933 6.4:+63130432 None:intergenic
TGTAACAACTTTATCGTTGT+AGG 0.335375 6.4:+63130460 None:intergenic
ATTTCTTACCCAGGATAGTA+TGG 0.344459 6.4:+63131308 None:intergenic
AGGTCAATAGAGAAAGATTA+AGG 0.358277 6.4:-63130337 MS.gene66343:CDS
GCTTTCCATCCAGCAAAGTA+TGG 0.363582 6.4:+63131886 None:intergenic
TATAGTCTTTCTAAAGATCT+TGG 0.364131 6.4:-63130515 MS.gene66343:CDS
CATGAAATAACTAACCCTTC+TGG 0.371377 6.4:-63131844 MS.gene66343:CDS
GAAAATTCTCCATACTTTGC+TGG 0.375504 6.4:-63131895 MS.gene66343:CDS
AAAAGCAATGGCAAGTTTCA+TGG 0.377867 6.4:-63131459 MS.gene66343:CDS
AACCCTAAACCAACCGGGTT+CGG 0.381536 6.4:+63130118 None:intergenic
CGTTGAATCGATATGCCTAA+TGG 0.381900 6.4:+63130701 None:intergenic
GGTAAAACTCGACATTCTTC+GGG 0.382775 6.4:+63130433 None:intergenic
GGGTTAGTTATTTCATGATA+AGG 0.383889 6.4:+63131850 None:intergenic
GATATGCCTAATGGATTTGA+TGG 0.385429 6.4:+63130710 None:intergenic
GGTTAGTTATTTCATGATAA+GGG 0.406672 6.4:+63131851 None:intergenic
GAGCAGGTGAATTGAAGCTT+TGG 0.427210 6.4:-63130199 MS.gene66343:CDS
ATTGAAGGTTTGAAAAGTGC+TGG 0.436332 6.4:-63130296 MS.gene66343:CDS
AAAACAAACCCTAAACCAAC+CGG 0.440582 6.4:+63130112 None:intergenic
ACTTTCATCCTTGCAAATCC+AGG 0.440957 6.4:-63131353 MS.gene66343:CDS
GATTTGATAGAGATTTAAGA+TGG 0.441356 6.4:-63130868 MS.gene66343:CDS
AGATATGAAATGATCATTGA+AGG 0.446464 6.4:-63130311 MS.gene66343:CDS
TCTTGTCATTGTTCCGAACC+CGG 0.453246 6.4:-63130131 MS.gene66343:CDS
CCCTTCTGGAGTTATACAAA+TGG 0.454742 6.4:-63131830 MS.gene66343:CDS
AGAGATTTAAGATGGAGAAC+TGG 0.456614 6.4:-63130860 MS.gene66343:CDS
GTTCCAACACCATACTATCC+TGG 0.457037 6.4:-63131317 MS.gene66343:CDS
AACAAGGACAGAAAGGATAC+AGG 0.467234 6.4:-63130029 MS.gene66343:CDS
TGGAGTTATACAAATGGGCT+TGG 0.477948 6.4:-63131824 MS.gene66343:CDS
TTTGAGCTATCACAATGGAT+TGG 0.480408 6.4:+63130824 None:intergenic
GATGAAAGTCAAGAGTTCAT+TGG 0.480725 6.4:+63131367 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.481569 6.4:-63130506 MS.gene66343:CDS
GCATTATTTCAAGACTATCA+TGG 0.482023 6.4:-63131497 MS.gene66343:CDS
CTTGTATTACTTTCCAACAT+TGG 0.485720 6.4:+63130221 None:intergenic
GAGGCATCAACATGGGGAAA+AGG 0.488094 6.4:-63131542 MS.gene66343:CDS
CCTTCTGGAGTTATACAAAT+GGG 0.492469 6.4:-63131829 MS.gene66343:CDS
ATTCTCCATACTTTGCTGGA+TGG 0.499110 6.4:-63131891 MS.gene66343:CDS
GTCATTGTTCCGAACCCGGT+TGG 0.507280 6.4:-63130127 MS.gene66343:CDS
CATGAATAACAAGGACAGAA+AGG 0.509122 6.4:-63130036 MS.gene66343:CDS
TTGGAAAGTAATACAAGAGC+AGG 0.510180 6.4:-63130215 MS.gene66343:CDS
GAATGCTTGAAAGGTAATGC+AGG 0.512496 6.4:-63130269 MS.gene66343:CDS
GAAAGAATTGTCCTCACTGC+TGG 0.516086 6.4:-63131401 MS.gene66343:CDS
TACCCAGGATAGTATGGTGT+TGG 0.517904 6.4:+63131314 None:intergenic
CCCATTTGTATAACTCCAGA+AGG 0.531454 6.4:+63131829 None:intergenic
TTCCAACACCATACTATCCT+GGG 0.532294 6.4:-63131316 MS.gene66343:intron
ATTGCAACTTCTGAAACTCA+TGG 0.536344 6.4:-63131919 MS.gene66343:CDS
GATGAATATGAGTCCAATGT+TGG 0.544594 6.4:-63130234 MS.gene66343:CDS
TTTCACTGATAATTACATCA+AGG 0.546541 6.4:-63130357 MS.gene66343:CDS
GCCTAATGGATTTGATGGGT+TGG 0.548657 6.4:+63130715 None:intergenic
GGAACAAGTAAAGCATCTCC+TGG 0.548786 6.4:+63131335 None:intergenic
TTGACGAGATTCAAGAATCT+CGG 0.556243 6.4:+63130568 None:intergenic
TGTCTCAGACGAAATCTACT+CGG 0.564955 6.4:-63130627 MS.gene66343:CDS
GGTCTACCAGGTTTCAGAGT+CGG 0.574668 6.4:-63130494 MS.gene66343:CDS
GTTAAGCTTAATATTTCACC+AGG 0.577298 6.4:-63130158 MS.gene66343:CDS
TAAATCATTCAGAAAAGCAA+TGG 0.583118 6.4:-63131471 MS.gene66343:CDS
GCTGGAATTGAATGCTTGAA+AGG 0.592728 6.4:-63130278 MS.gene66343:CDS
AACACTCAGAGGCATCAACA+TGG 0.595204 6.4:-63131550 MS.gene66343:CDS
ACCAACCCATCAAATCCATT+AGG 0.597602 6.4:-63130716 MS.gene66343:CDS
ATTGTTCCGACTCTGAAACC+TGG 0.601886 6.4:+63130488 None:intergenic
ACACTCAGAGGCATCAACAT+GGG 0.602352 6.4:-63131549 MS.gene66343:CDS
GTCTCAGACGAAATCTACTC+GGG 0.602761 6.4:-63130626 MS.gene66343:CDS
GCATCAACATGGGGAAAAGG+TGG 0.616595 6.4:-63131539 MS.gene66343:CDS
ACAAGGACAGAAAGGATACA+GGG 0.626149 6.4:-63130028 MS.gene66343:CDS
CACTCAGAGGCATCAACATG+GGG 0.626693 6.4:-63131548 MS.gene66343:CDS
AGTAGATTTCGTCTGAGACA+AGG 0.630503 6.4:+63130630 None:intergenic
AAGCATCTCCTGGATTTGCA+AGG 0.633214 6.4:+63131345 None:intergenic
GATTCAACGTTCAGTTCTCG+AGG 0.639409 6.4:-63130687 MS.gene66343:CDS
CCATTTGTATAACTCCAGAA+GGG 0.643005 6.4:+63131830 None:intergenic
ATATGCCTAATGGATTTGAT+GGG 0.644532 6.4:+63130711 None:intergenic
AATTGTTTGAGCTATCACAA+TGG 0.655921 6.4:+63130819 None:intergenic
ATATTTGAAAGTTCAACACA+AGG 0.665047 6.4:+63131940 None:intergenic
ACGTGATTTCATGAATAACA+AGG 0.666747 6.4:-63130045 MS.gene66343:CDS
AAACAAACCCTAAACCAACC+GGG 0.670128 6.4:+63130113 None:intergenic
TATTTGAAAGTTCAACACAA+GGG 0.671806 6.4:+63131941 None:intergenic
GAACAATGACAAGAACAACC+TGG 0.683485 6.4:+63130140 None:intergenic
CAGCAGTAGCACCAGCAGTG+AGG 0.696586 6.4:+63131390 None:intergenic
ATACTTGGAAGAACACTCAG+AGG 0.699650 6.4:-63131561 MS.gene66343:CDS
AATGCTTGAAAGGTAATGCA+GGG 0.724648 6.4:-63130268 MS.gene66343:CDS
ATCTCGTCAATACAAAGACG+CGG 0.725438 6.4:-63130555 MS.gene66343:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATAAAAATTTTGGTAT+AGG + chr6.4:63130772-63130791 None:intergenic 10.0%
!!! TATTTCTAAATTCGAAAATA+TGG - chr6.4:63130948-63130967 MS.gene66343:intron 15.0%
!!! TTTTTTGTTTGAAATTCTTT+AGG - chr6.4:63130383-63130402 MS.gene66343:CDS 15.0%
!! GACAATATAAGTACAATAAT+TGG - chr6.4:63131062-63131081 MS.gene66343:intron 20.0%
!! TCTCTTACCATAATTAATAA+TGG - chr6.4:63130740-63130759 MS.gene66343:CDS 20.0%
!!! ATAAAAATTTTGGTATAGGA+AGG + chr6.4:63130768-63130787 None:intergenic 20.0%
!!! GATATATGTACTTTAAAGAA+AGG - chr6.4:63130295-63130314 MS.gene66343:CDS 20.0%
!!! TATTTGCTTAAATGCATTTT+TGG - chr6.4:63130889-63130908 MS.gene66343:intron 20.0%
! AGATATGAAATGATCATTGA+AGG - chr6.4:63131678-63131697 MS.gene66343:intron 25.0%
! AGTTTCATGGAAAAAATTAG+AGG - chr6.4:63130543-63130562 MS.gene66343:CDS 25.0%
! ATATTTGAAAGTTCAACACA+AGG + chr6.4:63130052-63130071 None:intergenic 25.0%
! GAAGGTTCCATTATTAATTA+TGG + chr6.4:63130750-63130769 None:intergenic 25.0%
! GATTTGATAGAGATTTAAGA+TGG - chr6.4:63131121-63131140 MS.gene66343:intron 25.0%
! TAAATCATTCAGAAAAGCAA+TGG - chr6.4:63130518-63130537 MS.gene66343:CDS 25.0%
! TATTTGAAAGTTCAACACAA+GGG + chr6.4:63130051-63130070 None:intergenic 25.0%
! TTGTGTTTGTGAAGATATTA+AGG + chr6.4:63131580-63131599 None:intergenic 25.0%
! TTTCACTGATAATTACATCA+AGG - chr6.4:63131632-63131651 MS.gene66343:intron 25.0%
!! GGTTAGTTATTTCATGATAA+GGG + chr6.4:63130141-63130160 None:intergenic 25.0%
!! TATAGTCTTTCTAAAGATCT+TGG - chr6.4:63131474-63131493 MS.gene66343:CDS 25.0%
!!! CAAACTTTTTTTTTTTCCCT+TGG - chr6.4:63130806-63130825 MS.gene66343:CDS 25.0%
!!! TGATTTGCTTGAAAAATACT+TGG - chr6.4:63130413-63130432 MS.gene66343:CDS 25.0%
AAAGAGATAATTTCTTACCC+AGG + chr6.4:63130693-63130712 None:intergenic 30.0%
AATTGTTTGAGCTATCACAA+TGG + chr6.4:63131173-63131192 None:intergenic 30.0%
ACGTGATTTCATGAATAACA+AGG - chr6.4:63131944-63131963 MS.gene66343:CDS 30.0%
AGGTCAATAGAGAAAGATTA+AGG - chr6.4:63131652-63131671 MS.gene66343:intron 30.0%
CTTGTATTACTTTCCAACAT+TGG + chr6.4:63131771-63131790 None:intergenic 30.0%
GCATTATTTCAAGACTATCA+TGG - chr6.4:63130492-63130511 MS.gene66343:CDS 30.0%
GTTAAGCTTAATATTTCACC+AGG - chr6.4:63131831-63131850 MS.gene66343:CDS 30.0%
TGTAACAACTTTATCGTTGT+AGG + chr6.4:63131532-63131551 None:intergenic 30.0%
! ATGATAAGGGTTTTCATCAT+AGG + chr6.4:63130128-63130147 None:intergenic 30.0%
! TGCTTTTACTTTCATGTTCA+TGG + chr6.4:63131244-63131263 None:intergenic 30.0%
!! ATATGCCTAATGGATTTGAT+GGG + chr6.4:63131281-63131300 None:intergenic 30.0%
!! GGGTTAGTTATTTCATGATA+AGG + chr6.4:63130142-63130161 None:intergenic 30.0%
AAAACAAACCCTAAACCAAC+CGG + chr6.4:63131880-63131899 None:intergenic 35.0%
AAAAGCAATGGCAAGTTTCA+TGG - chr6.4:63130530-63130549 MS.gene66343:CDS 35.0%
AATGCTTGAAAGGTAATGCA+GGG - chr6.4:63131721-63131740 MS.gene66343:intron 35.0%
AGGTAAAACTCGACATTCTT+CGG + chr6.4:63131560-63131579 None:intergenic 35.0%
ATTTCTTACCCAGGATAGTA+TGG + chr6.4:63130684-63130703 None:intergenic 35.0%
CATGAAATAACTAACCCTTC+TGG - chr6.4:63130145-63130164 MS.gene66343:CDS 35.0%
CATGAATAACAAGGACAGAA+AGG - chr6.4:63131953-63131972 MS.gene66343:CDS 35.0%
CCATTTGTATAACTCCAGAA+GGG + chr6.4:63130162-63130181 None:intergenic 35.0%
GAAAATTCTCCATACTTTGC+TGG - chr6.4:63130094-63130113 MS.gene66343:CDS 35.0%
GATGAAAGTCAAGAGTTCAT+TGG + chr6.4:63130625-63130644 None:intergenic 35.0%
GATGAATATGAGTCCAATGT+TGG - chr6.4:63131755-63131774 MS.gene66343:intron 35.0%
TAAATGCTTGCAAGTTCCAA+GGG + chr6.4:63130825-63130844 None:intergenic 35.0%
TTAAATGCTTGCAAGTTCCA+AGG + chr6.4:63130826-63130845 None:intergenic 35.0%
TTGACGAGATTCAAGAATCT+CGG + chr6.4:63131424-63131443 None:intergenic 35.0%
TTGGAAAGTAATACAAGAGC+AGG - chr6.4:63131774-63131793 MS.gene66343:intron 35.0%
TTTGAGCTATCACAATGGAT+TGG + chr6.4:63131168-63131187 None:intergenic 35.0%
! AGAGATTTAAGATGGAGAAC+TGG - chr6.4:63131129-63131148 MS.gene66343:intron 35.0%
! ATTGAAGGTTTGAAAAGTGC+TGG - chr6.4:63131693-63131712 MS.gene66343:intron 35.0%
! TTTTCTTATATCGTTGACCC+AGG + chr6.4:63130242-63130261 None:intergenic 35.0%
!! ATTGCAACTTCTGAAACTCA+TGG - chr6.4:63130070-63130089 MS.gene66343:CDS 35.0%
!! CCTTCTGGAGTTATACAAAT+GGG - chr6.4:63130160-63130179 MS.gene66343:CDS 35.0%
!! GATATGCCTAATGGATTTGA+TGG + chr6.4:63131282-63131301 None:intergenic 35.0%
!! TCTTTTGAGTGCAAGTTGAA+GGG + chr6.4:63131922-63131941 None:intergenic 35.0%
!! TTCTTTTGAGTGCAAGTTGA+AGG + chr6.4:63131923-63131942 None:intergenic 35.0%
AAACAAACCCTAAACCAACC+GGG + chr6.4:63131879-63131898 None:intergenic 40.0%
AACAAGGACAGAAAGGATAC+AGG - chr6.4:63131960-63131979 MS.gene66343:CDS 40.0%
ACAAGGACAGAAAGGATACA+GGG - chr6.4:63131961-63131980 MS.gene66343:CDS 40.0%
ACCAACCCATCAAATCCATT+AGG - chr6.4:63131273-63131292 MS.gene66343:intron 40.0%
ACTTTCATCCTTGCAAATCC+AGG - chr6.4:63130636-63130655 MS.gene66343:CDS 40.0%
ATACTTGGAAGAACACTCAG+AGG - chr6.4:63130428-63130447 MS.gene66343:CDS 40.0%
ATCTCGTCAATACAAAGACG+CGG - chr6.4:63131434-63131453 MS.gene66343:CDS 40.0%
ATTCTCCATACTTTGCTGGA+TGG - chr6.4:63130098-63130117 MS.gene66343:CDS 40.0%
CCCATTTGTATAACTCCAGA+AGG + chr6.4:63130163-63130182 None:intergenic 40.0%
CGTTGAATCGATATGCCTAA+TGG + chr6.4:63131291-63131310 None:intergenic 40.0%
GAACAATGACAAGAACAACC+TGG + chr6.4:63131852-63131871 None:intergenic 40.0%
GAATGCTTGAAAGGTAATGC+AGG - chr6.4:63131720-63131739 MS.gene66343:intron 40.0%
GCTGGAATTGAATGCTTGAA+AGG - chr6.4:63131711-63131730 MS.gene66343:intron 40.0%
GGTAAAACTCGACATTCTTC+GGG + chr6.4:63131559-63131578 None:intergenic 40.0%
TCTAAAGATCTTGGTCTACC+AGG - chr6.4:63131483-63131502 MS.gene66343:CDS 40.0%
TGTCTCAGACGAAATCTACT+CGG - chr6.4:63131362-63131381 MS.gene66343:CDS 40.0%
TTCCAACACCATACTATCCT+GGG - chr6.4:63130673-63130692 MS.gene66343:CDS 40.0%
! AGTAGATTTCGTCTGAGACA+AGG + chr6.4:63131362-63131381 None:intergenic 40.0%
! TGGAGTTATACAAATGGGCT+TGG - chr6.4:63130165-63130184 MS.gene66343:CDS 40.0%
!! CCCTTCTGGAGTTATACAAA+TGG - chr6.4:63130159-63130178 MS.gene66343:CDS 40.0%
!! GTAATGCAGGGTTGTTTTGT+TGG - chr6.4:63131733-63131752 MS.gene66343:intron 40.0%
AAGCATCTCCTGGATTTGCA+AGG + chr6.4:63130647-63130666 None:intergenic 45.0%
CTTATATCGTTGACCCAGGT+CGG + chr6.4:63130238-63130257 None:intergenic 45.0%
GAAAGAATTGTCCTCACTGC+TGG - chr6.4:63130588-63130607 MS.gene66343:CDS 45.0%
GAGCAGGTGAATTGAAGCTT+TGG - chr6.4:63131790-63131809 MS.gene66343:intron 45.0%
GATTCAACGTTCAGTTCTCG+AGG - chr6.4:63131302-63131321 MS.gene66343:intron 45.0%
GCTTTCCATCCAGCAAAGTA+TGG + chr6.4:63130106-63130125 None:intergenic 45.0%
GTCTCAGACGAAATCTACTC+GGG - chr6.4:63131363-63131382 MS.gene66343:CDS 45.0%
GTTCCAACACCATACTATCC+TGG - chr6.4:63130672-63130691 MS.gene66343:CDS 45.0%
TACCCAGGATAGTATGGTGT+TGG + chr6.4:63130678-63130697 None:intergenic 45.0%
TCTTGTCATTGTTCCGAACC+CGG - chr6.4:63131858-63131877 MS.gene66343:CDS 45.0%
! GGAACAAGTAAAGCATCTCC+TGG + chr6.4:63130657-63130676 None:intergenic 45.0%
!! AACACTCAGAGGCATCAACA+TGG - chr6.4:63130439-63130458 MS.gene66343:CDS 45.0%
!! ACACTCAGAGGCATCAACAT+GGG - chr6.4:63130440-63130459 MS.gene66343:CDS 45.0%
!! ATTGTTCCGACTCTGAAACC+TGG + chr6.4:63131504-63131523 None:intergenic 45.0%
!! GCCTAATGGATTTGATGGGT+TGG + chr6.4:63131277-63131296 None:intergenic 45.0%
!!! AAACTATATTATAAAAATTT+TGG + chr6.4:63130778-63130797 None:intergenic 5.0%
AACCCTAAACCAACCGGGTT+CGG + chr6.4:63131874-63131893 None:intergenic 50.0%
GCATCAACATGGGGAAAAGG+TGG - chr6.4:63130450-63130469 MS.gene66343:CDS 50.0%
GGTCTACCAGGTTTCAGAGT+CGG - chr6.4:63131495-63131514 MS.gene66343:CDS 50.0%
! ACGTTTTCAGTGTCCGACCT+GGG - chr6.4:63130222-63130241 MS.gene66343:CDS 50.0%
! GAGGCATCAACATGGGGAAA+AGG - chr6.4:63130447-63130466 MS.gene66343:CDS 50.0%
!! CACTCAGAGGCATCAACATG+GGG - chr6.4:63130441-63130460 MS.gene66343:CDS 50.0%
!! TTCCGAACCCGGTTGGTTTA+GGG - chr6.4:63131869-63131888 MS.gene66343:CDS 50.0%
GTCATTGTTCCGAACCCGGT+TGG - chr6.4:63131862-63131881 MS.gene66343:CDS 55.0%
! GACGTTTTCAGTGTCCGACC+TGG - chr6.4:63130221-63130240 MS.gene66343:CDS 55.0%
!! GTTCCGAACCCGGTTGGTTT+AGG - chr6.4:63131868-63131887 MS.gene66343:CDS 55.0%
CAGCAGTAGCACCAGCAGTG+AGG + chr6.4:63130602-63130621 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 63130022 63131989 63130022 ID=MS.gene66343
chr6.4 mRNA 63130022 63131989 63130022 ID=MS.gene66343.t1;Parent=MS.gene66343
chr6.4 exon 63131813 63131989 63131813 ID=MS.gene66343.t1.exon1;Parent=MS.gene66343.t1
chr6.4 CDS 63131813 63131989 63131813 ID=cds.MS.gene66343.t1;Parent=MS.gene66343.t1
chr6.4 exon 63131317 63131606 63131317 ID=MS.gene66343.t1.exon2;Parent=MS.gene66343.t1
chr6.4 CDS 63131317 63131606 63131317 ID=cds.MS.gene66343.t1;Parent=MS.gene66343.t1
chr6.4 exon 63130022 63130889 63130022 ID=MS.gene66343.t1.exon3;Parent=MS.gene66343.t1
chr6.4 CDS 63130022 63130889 63130022 ID=cds.MS.gene66343.t1;Parent=MS.gene66343.t1
Gene Sequence

>MS.gene66343

ATGGGTATTGAGATTGAACAAGAACACCCTTGTGTTGAACTTTCAAATATTGCAACTTCTGAAACTCATGGAGAAAATTCTCCATACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATCATGAAATAACTAACCCTTCTGGAGTTATACAAATGGGCTTGGCAGAAAATCAAGTAAGTGTATAAATATATCGAAGACGTTTTCAGTGTCCGACCTGGGTCAACGATATAAGAAAAAAAAGTACATGTATCTGATATAAATTTTGATCAGATATATGTACTTTAAAGAAAGGAAAAAAATGTGACACTTAATTATTTTCATTAATTTCTAAGCTTATATTTAACTAGTTAACTAATATTTTTTGTTTGAAATTCTTTAGGTATCATTTGATTTGCTTGAAAAATACTTGGAAGAACACTCAGAGGCATCAACATGGGGAAAAGGTGGTTCAAGTTTTAGAGATAATGCATTATTTCAAGACTATCATGGTCTTAAATCATTCAGAAAAGCAATGGCAAGTTTCATGGAAAAAATTAGAGGAAATAAAGCAAAATTTGATTATGAAAGAATTGTCCTCACTGCTGGTGCTACTGCTGCCAATGAACTCTTGACTTTCATCCTTGCAAATCCAGGAGATGCTTTACTTGTTCCAACACCATACTATCCTGGGTAAGAAATTATCTCTTTTCACACTTCAATACATTTTTATATTTTTCTCTTACCATAATTAATAATGGAACCTTCCTATACCAAAATTTTTATAATATAGTTTCAAAATGTCAAACTTTTTTTTTTTCCCTTGGAACTTGCAAGCATTTAACTATTTGCTTTATGAGTATAATAAAAATATATTCTATATAATATATTTGCTTAAATGCATTTTTGGTCGTCTATTTTTACTAATTCATGATTTTAGCTCGTGTATTTCTAAATTCGAAAATATGGTCTTCATGTTTTCAAATTTAATGAGCACTAAAATCGTGAATCTATTAAAAAAATGATCAAACGAGTAATCATCTAAGCATAAAATTCGCGCGACAATATAAGTACAATAATTGGAAAATTAAACTAATGTATATATTTTTTGATTTTGCAGATTTGATAGAGATTTAAGATGGAGAACTGGTGTAAACATAGTTCCAATCCATTGTGATAGCTCAAACAATTTTCAAATCACACTTGAAGCATTAGAAACTGCATACAAAAATGCAGAATCCATGAACATGAAAGTAAAAGCAGTACTTATCACCAACCCATCAAATCCATTAGGCATATCGATTCAACGTTCAGTTCTCGAGGACATTCTCAATTTCGTGACTCGCAAGAACATACACCTTGTCTCAGACGAAATCTACTCGGGCTCAGTTTTCTCTTCACATGAATTCATAAGCATAGCCGAGATTCTTGAATCTCGTCAATACAAAGACGCGGAAAGATGTCACATTGTTTATAGTCTTTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTCGGAACAATTTATTCCTACAACGATAAAGTTGTTACAACAGCCCGAAGAATGTCGAGTTTTACCTTAATATCTTCACAAACACAACATCTTTTAGCATCAATGTTGTCAGATGAAAGTTTCACTGATAATTACATCAAGGTCAATAGAGAAAGATTAAGGAAAAGATATGAAATGATCATTGAAGGTTTGAAAAGTGCTGGAATTGAATGCTTGAAAGGTAATGCAGGGTTGTTTTGTTGGATGAATATGAGTCCAATGTTGGAAAGTAATACAAGAGCAGGTGAATTGAAGCTTTGGAATGAGATTTTGAATGAAGTTAAGCTTAATATTTCACCAGGTTGTTCTTGTCATTGTTCCGAACCCGGTTGGTTTAGGGTTTGTTTTGCAAATATGAGTGAAGAAACCCTTCAACTTGCACTCAAAAGAATACGTGATTTCATGAATAACAAGGACAGAAAGGATACAGGGATATAA
Protein sequence

>MS.gene66343.t1

MGIEIEQEHPCVELSNIATSETHGENSPYFAGWKAYDENPYHEITNPSGVIQMGLAENQVSFDLLEKYLEEHSEASTWGKGGSSFRDNALFQDYHGLKSFRKAMASFMEKIRGNKAKFDYERIVLTAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCDSSNNFQITLEALETAYKNAESMNMKVKAVLITNPSNPLGISIQRSVLEDILNFVTRKNIHLVSDEIYSGSVFSSHEFISIAEILESRQYKDAERCHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQHLLASMLSDESFTDNYIKVNRERLRKRYEMIIEGLKSAGIECLKGNAGLFCWMNMSPMLESNTRAGELKLWNEILNEVKLNISPGCSCHCSEPGWFRVCFANMSEETLQLALKRIRDFMNNKDRKDTGI