AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75059

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75059.t1 MTR_6g091760 98.874 444 5 0 1 444 1 444 0.0 920
MS.gene75059.t1 MTR_8g101750 81.922 437 71 2 1 437 1 429 0.0 758
MS.gene75059.t1 MTR_8g101820 76.201 437 83 3 1 437 1 416 0.0 698
MS.gene75059.t1 MTR_5g015020 61.111 432 159 5 14 436 4 435 0.0 558
MS.gene75059.t1 MTR_4g097540 60.325 431 161 4 14 434 4 434 0.0 552
MS.gene75059.t1 MTR_3g103550 58.645 428 172 2 14 436 2 429 0.0 549
MS.gene75059.t1 MTR_8g028600 56.308 428 185 2 14 440 11 437 0.0 526
MS.gene75059.t1 MTR_8g098930 55.530 443 190 5 1 438 1 441 0.0 521
MS.gene75059.t1 MTR_7g079080 56.279 430 187 1 14 442 11 440 0.0 520
MS.gene75059.t1 MTR_6g463260 44.213 432 238 2 12 441 102 532 4.77e-146 427
MS.gene75059.t1 MTR_7g079080 55.522 335 149 0 108 442 1 335 9.61e-140 405
MS.gene75059.t1 MTR_5g011400 44.010 409 221 3 26 430 84 488 1.60e-131 389
MS.gene75059.t1 MTR_2g090730 57.692 78 29 1 81 158 27 100 4.90e-22 94.0
MS.gene75059.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 8.24e-18 85.9
MS.gene75059.t1 MTR_8g023140 23.181 371 257 14 86 433 149 514 8.31e-18 86.3
MS.gene75059.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 9.01e-18 85.9
MS.gene75059.t1 MTR_4g011820 22.917 336 222 12 105 431 75 382 1.40e-14 75.5
MS.gene75059.t1 MTR_8g061360 21.168 411 276 15 46 440 38 416 1.34e-11 66.6
MS.gene75059.t1 MTR_7g106830 19.637 331 243 10 114 436 151 466 6.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75059.t1 AT4G26200 75.638 431 102 3 12 439 16 446 0.0 665
MS.gene75059.t1 AT4G37770 60.563 426 164 2 14 435 4 429 0.0 557
MS.gene75059.t1 AT4G08040 60.798 426 166 1 14 438 2 427 0.0 554
MS.gene75059.t1 AT5G65800 58.837 430 173 2 13 438 3 432 0.0 545
MS.gene75059.t1 AT3G49700 58.333 432 176 2 13 440 3 434 0.0 542
MS.gene75059.t1 AT2G22810 58.333 432 175 3 12 438 2 433 0.0 538
MS.gene75059.t1 AT3G61510 58.531 422 171 2 14 434 11 429 0.0 536
MS.gene75059.t1 AT4G11280 58.670 421 173 1 14 433 15 435 0.0 530
MS.gene75059.t1 AT1G01480 54.524 431 192 3 14 440 12 442 2.17e-178 508
MS.gene75059.t1 AT1G01480 53.571 336 153 2 108 440 1 336 1.44e-136 397
MS.gene75059.t1 AT5G51690 43.396 424 237 2 12 433 67 489 2.31e-136 401
MS.gene75059.t1 AT1G62960 42.787 409 229 3 24 431 143 547 1.58e-127 380
MS.gene75059.t1 AT5G51690 46.064 343 184 1 89 431 23 364 3.86e-121 358
MS.gene75059.t1 AT1G72330 24.865 370 252 14 86 433 165 530 6.38e-20 92.8
MS.gene75059.t1 AT1G23310 26.259 278 185 10 91 352 103 376 8.34e-20 91.7
MS.gene75059.t1 AT1G23310 26.259 278 185 10 91 352 103 376 1.14e-19 91.7
MS.gene75059.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene75059.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene75059.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene75059.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene75059.t1 AT1G72330 24.021 383 252 14 86 433 165 543 4.23e-18 87.0
MS.gene75059.t1 AT1G72330 25.573 262 181 8 86 336 165 423 1.10e-17 85.5
MS.gene75059.t1 AT1G17290 23.826 298 207 11 86 367 168 461 3.02e-17 84.3
MS.gene75059.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene75059.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene75059.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene75059.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene75059.t1 AT2G24850 24.000 350 232 14 96 433 92 419 1.48e-15 79.0
MS.gene75059.t1 AT1G77670 24.458 323 214 11 121 437 141 439 1.63e-14 75.5
MS.gene75059.t1 AT4G23600 23.056 360 236 14 96 440 78 411 6.37e-14 73.9
MS.gene75059.t1 AT5G36160 22.096 353 234 13 96 433 85 411 1.36e-13 72.8
MS.gene75059.t1 AT2G20610 22.284 359 240 14 96 440 109 442 2.40e-12 68.9
MS.gene75059.t1 AT4G23600 23.263 331 215 13 125 440 1 307 9.10e-12 66.2
MS.gene75059.t1 AT2G20610 23.810 273 177 10 96 358 109 360 1.01e-11 67.0
MS.gene75059.t1 AT4G23600 23.264 288 188 10 96 370 78 345 3.11e-11 65.1

Find 80 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCGAACCCGGTTGGTTT+AGG 0.229798 6.3:-83763469 MS.gene75059:CDS
TTCCGAACCCGGTTGGTTTA+GGG 0.281999 6.3:-83763468 MS.gene75059:CDS
TTGTGTTTGTGAAGATATTA+AGG 0.307747 6.3:+83763760 None:intergenic
AGGTAAAACTCGACATTCTT+CGG 0.310933 6.3:+83763780 None:intergenic
TGTAACAACTTTATCGTTGT+AGG 0.335375 6.3:+83763808 None:intergenic
ATTTCTTACCCAGGATAGTA+TGG 0.344459 6.3:+83764656 None:intergenic
AGGTCAATAGAGAAAGATTA+AGG 0.358277 6.3:-83763685 MS.gene75059:CDS
GCTTTCCATCCAGCAAAGTA+TGG 0.363582 6.3:+83765234 None:intergenic
TATAGTCTTTCTAAAGATCT+TGG 0.364131 6.3:-83763863 MS.gene75059:CDS
CATGAAATAACTAACCCTTC+TGG 0.371377 6.3:-83765192 MS.gene75059:CDS
GAAAATTCTCCATACTTTGC+TGG 0.375504 6.3:-83765243 MS.gene75059:CDS
AAAAGCAATGGCAAGTTTCA+TGG 0.377867 6.3:-83764807 MS.gene75059:CDS
AACCCTAAACCAACCGGGTT+CGG 0.381536 6.3:+83763466 None:intergenic
CGTTGAATCGATATGCCTAA+TGG 0.381900 6.3:+83764049 None:intergenic
GGTAAAACTCGACATTCTTC+GGG 0.382775 6.3:+83763781 None:intergenic
GGGTTAGTTATTTCATGATA+AGG 0.383889 6.3:+83765198 None:intergenic
GATATGCCTAATGGATTTGA+TGG 0.385429 6.3:+83764058 None:intergenic
GGTTAGTTATTTCATGATAA+GGG 0.406672 6.3:+83765199 None:intergenic
GAGCAGGTGAATTGAAGCTT+TGG 0.427210 6.3:-83763547 MS.gene75059:CDS
ATTGAAGGTTTGAAAAGTGC+TGG 0.436332 6.3:-83763644 MS.gene75059:CDS
AAAACAAACCCTAAACCAAC+CGG 0.440582 6.3:+83763460 None:intergenic
ACTTTCATCCTTGCAAATCC+AGG 0.440957 6.3:-83764701 MS.gene75059:CDS
GATTTGATAGAGATTTAAGA+TGG 0.441356 6.3:-83764216 MS.gene75059:CDS
AGATATGAAATGATCATTGA+AGG 0.446464 6.3:-83763659 MS.gene75059:CDS
TCTTGTCATTGTTCCGAACC+CGG 0.453246 6.3:-83763479 MS.gene75059:CDS
CCCTTCTGGAGTTATACAAA+TGG 0.454742 6.3:-83765178 MS.gene75059:CDS
AGAGATTTAAGATGGAGAAC+TGG 0.456614 6.3:-83764208 MS.gene75059:CDS
GTTCCAACACCATACTATCC+TGG 0.457037 6.3:-83764665 MS.gene75059:CDS
AACAAGGACAGAAAGGATAC+AGG 0.467234 6.3:-83763377 MS.gene75059:CDS
TGGAGTTATACAAATGGGCT+TGG 0.477948 6.3:-83765172 MS.gene75059:CDS
TTTGAGCTATCACAATGGAT+TGG 0.480408 6.3:+83764172 None:intergenic
GATGAAAGTCAAGAGTTCAT+TGG 0.480725 6.3:+83764715 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.481569 6.3:-83763854 MS.gene75059:CDS
GCATTATTTCAAGACTATCA+TGG 0.482023 6.3:-83764845 MS.gene75059:CDS
CTTGTATTACTTTCCAACAT+TGG 0.485720 6.3:+83763569 None:intergenic
GAGGCATCAACATGGGGAAA+AGG 0.488094 6.3:-83764890 MS.gene75059:CDS
CCTTCTGGAGTTATACAAAT+GGG 0.492469 6.3:-83765177 MS.gene75059:CDS
ATTCTCCATACTTTGCTGGA+TGG 0.499110 6.3:-83765239 MS.gene75059:CDS
GTCATTGTTCCGAACCCGGT+TGG 0.507280 6.3:-83763475 MS.gene75059:CDS
CATGAATAACAAGGACAGAA+AGG 0.509122 6.3:-83763384 MS.gene75059:CDS
TTGGAAAGTAATACAAGAGC+AGG 0.510180 6.3:-83763563 MS.gene75059:CDS
GAATGCTTGAAAGGTAATGC+AGG 0.512496 6.3:-83763617 MS.gene75059:CDS
GAAAGAATTGTCCTCACTGC+TGG 0.516086 6.3:-83764749 MS.gene75059:CDS
TACCCAGGATAGTATGGTGT+TGG 0.517904 6.3:+83764662 None:intergenic
CCCATTTGTATAACTCCAGA+AGG 0.531454 6.3:+83765177 None:intergenic
TTCCAACACCATACTATCCT+GGG 0.532294 6.3:-83764664 MS.gene75059:intron
ATTGCAACTTCTGAAACTCA+TGG 0.536344 6.3:-83765267 MS.gene75059:CDS
GATGAATATGAGTCCAATGT+TGG 0.544594 6.3:-83763582 MS.gene75059:CDS
TTTCACTGATAATTACATCA+AGG 0.546541 6.3:-83763705 MS.gene75059:CDS
GCCTAATGGATTTGATGGGT+TGG 0.548657 6.3:+83764063 None:intergenic
GGAACAAGTAAAGCATCTCC+TGG 0.548786 6.3:+83764683 None:intergenic
TTGACGAGATTCAAGAATCT+CGG 0.556243 6.3:+83763916 None:intergenic
TGTCTCAGACGAAATCTACT+CGG 0.564955 6.3:-83763975 MS.gene75059:CDS
GGTCTACCAGGTTTCAGAGT+CGG 0.574668 6.3:-83763842 MS.gene75059:CDS
GTTAAGCTTAATATTTCACC+AGG 0.577298 6.3:-83763506 MS.gene75059:CDS
TAAATCATTCAGAAAAGCAA+TGG 0.583118 6.3:-83764819 MS.gene75059:CDS
GCTGGAATTGAATGCTTGAA+AGG 0.592728 6.3:-83763626 MS.gene75059:CDS
AACACTCAGAGGCATCAACA+TGG 0.595204 6.3:-83764898 MS.gene75059:CDS
ACCAACCCATCAAATCCATT+AGG 0.597602 6.3:-83764064 MS.gene75059:CDS
ATTGTTCCGACTCTGAAACC+TGG 0.601886 6.3:+83763836 None:intergenic
ACACTCAGAGGCATCAACAT+GGG 0.602352 6.3:-83764897 MS.gene75059:CDS
GTCTCAGACGAAATCTACTC+GGG 0.602761 6.3:-83763974 MS.gene75059:CDS
GCATCAACATGGGGAAAAGG+TGG 0.616595 6.3:-83764887 MS.gene75059:CDS
ACAAGGACAGAAAGGATACA+GGG 0.626149 6.3:-83763376 MS.gene75059:CDS
CACTCAGAGGCATCAACATG+GGG 0.626693 6.3:-83764896 MS.gene75059:CDS
AGTAGATTTCGTCTGAGACA+AGG 0.630503 6.3:+83763978 None:intergenic
AAGCATCTCCTGGATTTGCA+AGG 0.633214 6.3:+83764693 None:intergenic
GATTCAACGTTCAGTTCTCG+AGG 0.639409 6.3:-83764035 MS.gene75059:CDS
CCATTTGTATAACTCCAGAA+GGG 0.643005 6.3:+83765178 None:intergenic
ATATGCCTAATGGATTTGAT+GGG 0.644532 6.3:+83764059 None:intergenic
AATTGTTTGAGCTATCACAA+TGG 0.655921 6.3:+83764167 None:intergenic
ATATTTGAAAGTTCAACACA+AGG 0.665047 6.3:+83765288 None:intergenic
ACGTGATTTCATGAATAACA+AGG 0.666747 6.3:-83763393 MS.gene75059:CDS
AAACAAACCCTAAACCAACC+GGG 0.670128 6.3:+83763461 None:intergenic
TATTTGAAAGTTCAACACAA+GGG 0.671806 6.3:+83765289 None:intergenic
GAACAATGACAAGAACAACC+TGG 0.683485 6.3:+83763488 None:intergenic
CAGCAGTAGCACCAGCAGTG+AGG 0.696586 6.3:+83764738 None:intergenic
ATACTTGGAAGAACACTCAG+AGG 0.699650 6.3:-83764909 MS.gene75059:CDS
AATGCTTGAAAGGTAATGCA+GGG 0.724648 6.3:-83763616 MS.gene75059:CDS
ATCTCGTCAATACAAAGACG+CGG 0.725438 6.3:-83763903 MS.gene75059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATAAAAATTTTGGTAT+AGG + chr6.3:83764120-83764139 None:intergenic 10.0%
!!! TATTTCTAAATTCGAAAATA+TGG - chr6.3:83764296-83764315 MS.gene75059:intron 15.0%
!!! TTTTTTGTTTGAAATTCTTT+AGG - chr6.3:83763731-83763750 MS.gene75059:CDS 15.0%
!! GACAATATAAGTACAATAAT+TGG - chr6.3:83764410-83764429 MS.gene75059:intron 20.0%
!! TCTCTTACCATAATTAATAA+TGG - chr6.3:83764088-83764107 MS.gene75059:CDS 20.0%
!!! ATAAAAATTTTGGTATAGGA+AGG + chr6.3:83764116-83764135 None:intergenic 20.0%
!!! GATATATGTACTTTAAAGAA+AGG - chr6.3:83763643-83763662 MS.gene75059:CDS 20.0%
!!! TATTTGCTTAAATGCATTTT+TGG - chr6.3:83764237-83764256 MS.gene75059:intron 20.0%
! AGATATGAAATGATCATTGA+AGG - chr6.3:83765026-83765045 MS.gene75059:intron 25.0%
! AGTTTCATGGAAAAAATTAG+AGG - chr6.3:83763891-83763910 MS.gene75059:CDS 25.0%
! ATATTTGAAAGTTCAACACA+AGG + chr6.3:83763400-83763419 None:intergenic 25.0%
! GAAGGTTCCATTATTAATTA+TGG + chr6.3:83764098-83764117 None:intergenic 25.0%
! GATTTGATAGAGATTTAAGA+TGG - chr6.3:83764469-83764488 MS.gene75059:intron 25.0%
! TAAATCATTCAGAAAAGCAA+TGG - chr6.3:83763866-83763885 MS.gene75059:CDS 25.0%
! TATTTGAAAGTTCAACACAA+GGG + chr6.3:83763399-83763418 None:intergenic 25.0%
! TTGTGTTTGTGAAGATATTA+AGG + chr6.3:83764928-83764947 None:intergenic 25.0%
! TTTCACTGATAATTACATCA+AGG - chr6.3:83764980-83764999 MS.gene75059:intron 25.0%
!! GGTTAGTTATTTCATGATAA+GGG + chr6.3:83763489-83763508 None:intergenic 25.0%
!! TATAGTCTTTCTAAAGATCT+TGG - chr6.3:83764822-83764841 MS.gene75059:CDS 25.0%
!!! CAAACTTTTTTTTTTTCCCT+TGG - chr6.3:83764154-83764173 MS.gene75059:CDS 25.0%
!!! TGATTTGCTTGAAAAATACT+TGG - chr6.3:83763761-83763780 MS.gene75059:CDS 25.0%
AAAGAGATAATTTCTTACCC+AGG + chr6.3:83764041-83764060 None:intergenic 30.0%
AATTGTTTGAGCTATCACAA+TGG + chr6.3:83764521-83764540 None:intergenic 30.0%
ACGTGATTTCATGAATAACA+AGG - chr6.3:83765292-83765311 MS.gene75059:CDS 30.0%
AGGTCAATAGAGAAAGATTA+AGG - chr6.3:83765000-83765019 MS.gene75059:intron 30.0%
CTTGTATTACTTTCCAACAT+TGG + chr6.3:83765119-83765138 None:intergenic 30.0%
GCATTATTTCAAGACTATCA+TGG - chr6.3:83763840-83763859 MS.gene75059:CDS 30.0%
GTTAAGCTTAATATTTCACC+AGG - chr6.3:83765179-83765198 MS.gene75059:CDS 30.0%
TGTAACAACTTTATCGTTGT+AGG + chr6.3:83764880-83764899 None:intergenic 30.0%
! ATGATAAGGGTTTTCATCAT+AGG + chr6.3:83763476-83763495 None:intergenic 30.0%
! TGCTTTTACTTTCATGTTCA+TGG + chr6.3:83764592-83764611 None:intergenic 30.0%
! TTTTCTTATATCATTGACCC+AGG + chr6.3:83763590-83763609 None:intergenic 30.0%
!! ATATGCCTAATGGATTTGAT+GGG + chr6.3:83764629-83764648 None:intergenic 30.0%
!! GGGTTAGTTATTTCATGATA+AGG + chr6.3:83763490-83763509 None:intergenic 30.0%
AAAACAAACCCTAAACCAAC+CGG + chr6.3:83765228-83765247 None:intergenic 35.0%
AAAAGCAATGGCAAGTTTCA+TGG - chr6.3:83763878-83763897 MS.gene75059:CDS 35.0%
AATGCTTGAAAGGTAATGCA+GGG - chr6.3:83765069-83765088 MS.gene75059:intron 35.0%
AGGTAAAACTCGACATTCTT+CGG + chr6.3:83764908-83764927 None:intergenic 35.0%
ATTTCTTACCCAGGATAGTA+TGG + chr6.3:83764032-83764051 None:intergenic 35.0%
CATGAAATAACTAACCCTTC+TGG - chr6.3:83763493-83763512 MS.gene75059:CDS 35.0%
CATGAATAACAAGGACAGAA+AGG - chr6.3:83765301-83765320 MS.gene75059:CDS 35.0%
CCATTTGTATAACTCCAGAA+GGG + chr6.3:83763510-83763529 None:intergenic 35.0%
GAAAATTCTCCATACTTTGC+TGG - chr6.3:83763442-83763461 MS.gene75059:CDS 35.0%
GATGAAAGTCAAGAGTTCAT+TGG + chr6.3:83763973-83763992 None:intergenic 35.0%
GATGAATATGAGTCCAATGT+TGG - chr6.3:83765103-83765122 MS.gene75059:intron 35.0%
TAAATGCTTGCAAGTTCCAA+GGG + chr6.3:83764173-83764192 None:intergenic 35.0%
TTAAATGCTTGCAAGTTCCA+AGG + chr6.3:83764174-83764193 None:intergenic 35.0%
TTGACGAGATTCAAGAATCT+CGG + chr6.3:83764772-83764791 None:intergenic 35.0%
TTGGAAAGTAATACAAGAGC+AGG - chr6.3:83765122-83765141 MS.gene75059:intron 35.0%
TTTGAGCTATCACAATGGAT+TGG + chr6.3:83764516-83764535 None:intergenic 35.0%
! AGAGATTTAAGATGGAGAAC+TGG - chr6.3:83764477-83764496 MS.gene75059:intron 35.0%
! ATTGAAGGTTTGAAAAGTGC+TGG - chr6.3:83765041-83765060 MS.gene75059:intron 35.0%
!! ATTGCAACTTCTGAAACTCA+TGG - chr6.3:83763418-83763437 MS.gene75059:CDS 35.0%
!! CCTTCTGGAGTTATACAAAT+GGG - chr6.3:83763508-83763527 MS.gene75059:CDS 35.0%
!! GATATGCCTAATGGATTTGA+TGG + chr6.3:83764630-83764649 None:intergenic 35.0%
!! TCTTTTGAGTGCAAGTTGAA+GGG + chr6.3:83765270-83765289 None:intergenic 35.0%
!! TTCTTTTGAGTGCAAGTTGA+AGG + chr6.3:83765271-83765290 None:intergenic 35.0%
AAACAAACCCTAAACCAACC+GGG + chr6.3:83765227-83765246 None:intergenic 40.0%
AACAAGGACAGAAAGGATAC+AGG - chr6.3:83765308-83765327 MS.gene75059:CDS 40.0%
ACAAGGACAGAAAGGATACA+GGG - chr6.3:83765309-83765328 MS.gene75059:CDS 40.0%
ACCAACCCATCAAATCCATT+AGG - chr6.3:83764621-83764640 MS.gene75059:intron 40.0%
ACTTTCATCCTTGCAAATCC+AGG - chr6.3:83763984-83764003 MS.gene75059:CDS 40.0%
ATACTTGGAAGAACACTCAG+AGG - chr6.3:83763776-83763795 MS.gene75059:CDS 40.0%
ATCTCGTCAATACAAAGACG+CGG - chr6.3:83764782-83764801 MS.gene75059:CDS 40.0%
ATTCTCCATACTTTGCTGGA+TGG - chr6.3:83763446-83763465 MS.gene75059:CDS 40.0%
CCCATTTGTATAACTCCAGA+AGG + chr6.3:83763511-83763530 None:intergenic 40.0%
CGTTGAATCGATATGCCTAA+TGG + chr6.3:83764639-83764658 None:intergenic 40.0%
CTTATATCATTGACCCAGGT+CGG + chr6.3:83763586-83763605 None:intergenic 40.0%
GAACAATGACAAGAACAACC+TGG + chr6.3:83765200-83765219 None:intergenic 40.0%
GAATGCTTGAAAGGTAATGC+AGG - chr6.3:83765068-83765087 MS.gene75059:intron 40.0%
GCTGGAATTGAATGCTTGAA+AGG - chr6.3:83765059-83765078 MS.gene75059:intron 40.0%
GGTAAAACTCGACATTCTTC+GGG + chr6.3:83764907-83764926 None:intergenic 40.0%
TCTAAAGATCTTGGTCTACC+AGG - chr6.3:83764831-83764850 MS.gene75059:CDS 40.0%
TGTCTCAGACGAAATCTACT+CGG - chr6.3:83764710-83764729 MS.gene75059:CDS 40.0%
TTCCAACACCATACTATCCT+GGG - chr6.3:83764021-83764040 MS.gene75059:CDS 40.0%
! AGTAGATTTCGTCTGAGACA+AGG + chr6.3:83764710-83764729 None:intergenic 40.0%
! TGGAGTTATACAAATGGGCT+TGG - chr6.3:83763513-83763532 MS.gene75059:CDS 40.0%
!! CCCTTCTGGAGTTATACAAA+TGG - chr6.3:83763507-83763526 MS.gene75059:CDS 40.0%
!! GTAATGCAGGGTTGTTTTGT+TGG - chr6.3:83765081-83765100 MS.gene75059:intron 40.0%
AAGCATCTCCTGGATTTGCA+AGG + chr6.3:83763995-83764014 None:intergenic 45.0%
GAAAGAATTGTCCTCACTGC+TGG - chr6.3:83763936-83763955 MS.gene75059:CDS 45.0%
GAGCAGGTGAATTGAAGCTT+TGG - chr6.3:83765138-83765157 MS.gene75059:intron 45.0%
GATTCAACGTTCAGTTCTCG+AGG - chr6.3:83764650-83764669 MS.gene75059:intron 45.0%
GCTTTCCATCCAGCAAAGTA+TGG + chr6.3:83763454-83763473 None:intergenic 45.0%
GTCTCAGACGAAATCTACTC+GGG - chr6.3:83764711-83764730 MS.gene75059:CDS 45.0%
GTTCCAACACCATACTATCC+TGG - chr6.3:83764020-83764039 MS.gene75059:CDS 45.0%
TACCCAGGATAGTATGGTGT+TGG + chr6.3:83764026-83764045 None:intergenic 45.0%
TCTTGTCATTGTTCCGAACC+CGG - chr6.3:83765206-83765225 MS.gene75059:CDS 45.0%
! GGAACAAGTAAAGCATCTCC+TGG + chr6.3:83764005-83764024 None:intergenic 45.0%
!! AACACTCAGAGGCATCAACA+TGG - chr6.3:83763787-83763806 MS.gene75059:CDS 45.0%
!! ACACTCAGAGGCATCAACAT+GGG - chr6.3:83763788-83763807 MS.gene75059:CDS 45.0%
!! ATTGTTCCGACTCTGAAACC+TGG + chr6.3:83764852-83764871 None:intergenic 45.0%
!! GCCTAATGGATTTGATGGGT+TGG + chr6.3:83764625-83764644 None:intergenic 45.0%
!!! AAACTATATTATAAAAATTT+TGG + chr6.3:83764126-83764145 None:intergenic 5.0%
AACCCTAAACCAACCGGGTT+CGG + chr6.3:83765222-83765241 None:intergenic 50.0%
GCATCAACATGGGGAAAAGG+TGG - chr6.3:83763798-83763817 MS.gene75059:CDS 50.0%
GGTCTACCAGGTTTCAGAGT+CGG - chr6.3:83764843-83764862 MS.gene75059:CDS 50.0%
! ACGTTTTCAGTGTCCGACCT+GGG - chr6.3:83763570-83763589 MS.gene75059:CDS 50.0%
! GAGGCATCAACATGGGGAAA+AGG - chr6.3:83763795-83763814 MS.gene75059:CDS 50.0%
!! CACTCAGAGGCATCAACATG+GGG - chr6.3:83763789-83763808 MS.gene75059:CDS 50.0%
!! TTCCGAACCCGGTTGGTTTA+GGG - chr6.3:83765217-83765236 MS.gene75059:CDS 50.0%
GTCATTGTTCCGAACCCGGT+TGG - chr6.3:83765210-83765229 MS.gene75059:CDS 55.0%
! GACGTTTTCAGTGTCCGACC+TGG - chr6.3:83763569-83763588 MS.gene75059:CDS 55.0%
!! GTTCCGAACCCGGTTGGTTT+AGG - chr6.3:83765216-83765235 MS.gene75059:CDS 55.0%
CAGCAGTAGCACCAGCAGTG+AGG + chr6.3:83763950-83763969 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 83763370 83765337 83763370 ID=MS.gene75059
chr6.3 mRNA 83763370 83765337 83763370 ID=MS.gene75059.t1;Parent=MS.gene75059
chr6.3 exon 83765161 83765337 83765161 ID=MS.gene75059.t1.exon1;Parent=MS.gene75059.t1
chr6.3 CDS 83765161 83765337 83765161 ID=cds.MS.gene75059.t1;Parent=MS.gene75059.t1
chr6.3 exon 83764665 83764954 83764665 ID=MS.gene75059.t1.exon2;Parent=MS.gene75059.t1
chr6.3 CDS 83764665 83764954 83764665 ID=cds.MS.gene75059.t1;Parent=MS.gene75059.t1
chr6.3 exon 83763370 83764237 83763370 ID=MS.gene75059.t1.exon3;Parent=MS.gene75059.t1
chr6.3 CDS 83763370 83764237 83763370 ID=cds.MS.gene75059.t1;Parent=MS.gene75059.t1
Gene Sequence

>MS.gene75059

ATGGGTATTGAGATTGAACAAGAACACCCTTGTGTTGAACTTTCAAATATTGCAACTTCTGAAACTCATGGAGAAAATTCTCCATACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATCATGAAATAACTAACCCTTCTGGAGTTATACAAATGGGCTTGGCAGAAAATCAAGTAAGTGTATAAATATATCGAAGACGTTTTCAGTGTCCGACCTGGGTCAATGATATAAGAAAAAAAAGTACATGTATCTGATATAAATTTTGATCAGATATATGTACTTTAAAGAAAGGAAAAAAATGTGACACTTAATTATTTTCATTAATTTCTAAGCTTATATTTAACTAGTTAACTAATATTTTTTGTTTGAAATTCTTTAGGTATCATTTGATTTGCTTGAAAAATACTTGGAAGAACACTCAGAGGCATCAACATGGGGAAAAGGTGGTTCAAGTTTTAGAGATAATGCATTATTTCAAGACTATCATGGTCTTAAATCATTCAGAAAAGCAATGGCAAGTTTCATGGAAAAAATTAGAGGAAATAAAGCAAAATTTGATTATGAAAGAATTGTCCTCACTGCTGGTGCTACTGCTGCCAATGAACTCTTGACTTTCATCCTTGCAAATCCAGGAGATGCTTTACTTGTTCCAACACCATACTATCCTGGGTAAGAAATTATCTCTTTTCACACTTCAATACATTTTTATATTTTTCTCTTACCATAATTAATAATGGAACCTTCCTATACCAAAATTTTTATAATATAGTTTCAAAATGTCAAACTTTTTTTTTTTCCCTTGGAACTTGCAAGCATTTAACTATTTGCTTTATGAGTATAATAAAAATATATTCTATATAATATATTTGCTTAAATGCATTTTTGGTCGTCTATTTTTACTAATTCATGATTTTAGCTCGTGTATTTCTAAATTCGAAAATATGGTCTTCATGTTTTCAAATTTAATGAGCACTAAAATCGTGAATCTATTAAAAAAATGATCAAACGAGTAATCATCTAAGCATAAAATTCGCGCGACAATATAAGTACAATAATTGGAAAATTAAACTAATGTATATATTTTTTGATTTTGCAGATTTGATAGAGATTTAAGATGGAGAACTGGTGTAAACATAGTTCCAATCCATTGTGATAGCTCAAACAATTTTCAAATCACACTTGAAGCATTAGAAACTGCATACAAAAATGCAGAATCCATGAACATGAAAGTAAAAGCAGTACTTATCACCAACCCATCAAATCCATTAGGCATATCGATTCAACGTTCAGTTCTCGAGGACATTCTCAATTTCGTGACTCGCAAGAACATACACCTTGTCTCAGACGAAATCTACTCGGGCTCAGTTTTCTCTTCACATGAATTCATAAGCATAGCCGAGATTCTTGAATCTCGTCAATACAAAGACGCGGAAAGATGTCACATTGTTTATAGTCTTTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTCGGAACAATTTATTCCTACAACGATAAAGTTGTTACAACAGCCCGAAGAATGTCGAGTTTTACCTTAATATCTTCACAAACACAACATCTTTTAGCATCAATGTTGTCAGATGAAAGTTTCACTGATAATTACATCAAGGTCAATAGAGAAAGATTAAGGAAAAGATATGAAATGATCATTGAAGGTTTGAAAAGTGCTGGAATTGAATGCTTGAAAGGTAATGCAGGGTTGTTTTGTTGGATGAATATGAGTCCAATGTTGGAAAGTAATACAAGAGCAGGTGAATTGAAGCTTTGGAATGAGATTTTGAATGAAGTTAAGCTTAATATTTCACCAGGTTGTTCTTGTCATTGTTCCGAACCCGGTTGGTTTAGGGTTTGTTTTGCAAATATGAGTGAAGAAACCCTTCAACTTGCACTCAAAAGAATACGTGATTTCATGAATAACAAGGACAGAAAGGATACAGGGATATAA
Protein sequence

>MS.gene75059.t1

MGIEIEQEHPCVELSNIATSETHGENSPYFAGWKAYDENPYHEITNPSGVIQMGLAENQVSFDLLEKYLEEHSEASTWGKGGSSFRDNALFQDYHGLKSFRKAMASFMEKIRGNKAKFDYERIVLTAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCDSSNNFQITLEALETAYKNAESMNMKVKAVLITNPSNPLGISIQRSVLEDILNFVTRKNIHLVSDEIYSGSVFSSHEFISIAEILESRQYKDAERCHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQHLLASMLSDESFTDNYIKVNRERLRKRYEMIIEGLKSAGIECLKGNAGLFCWMNMSPMLESNTRAGELKLWNEILNEVKLNISPGCSCHCSEPGWFRVCFANMSEETLQLALKRIRDFMNNKDRKDTGI