AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66342

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66342.t1 MTR_6g091760 98.874 444 5 0 1 444 1 444 0.0 920
MS.gene66342.t1 MTR_8g101750 81.922 437 71 2 1 437 1 429 0.0 758
MS.gene66342.t1 MTR_8g101820 76.201 437 83 3 1 437 1 416 0.0 698
MS.gene66342.t1 MTR_5g015020 61.111 432 159 5 14 436 4 435 0.0 558
MS.gene66342.t1 MTR_4g097540 60.325 431 161 4 14 434 4 434 0.0 552
MS.gene66342.t1 MTR_3g103550 58.645 428 172 2 14 436 2 429 0.0 549
MS.gene66342.t1 MTR_8g028600 56.308 428 185 2 14 440 11 437 0.0 526
MS.gene66342.t1 MTR_8g098930 55.530 443 190 5 1 438 1 441 0.0 521
MS.gene66342.t1 MTR_7g079080 56.279 430 187 1 14 442 11 440 0.0 520
MS.gene66342.t1 MTR_6g463260 44.213 432 238 2 12 441 102 532 4.77e-146 427
MS.gene66342.t1 MTR_7g079080 55.522 335 149 0 108 442 1 335 9.61e-140 405
MS.gene66342.t1 MTR_5g011400 44.010 409 221 3 26 430 84 488 1.60e-131 389
MS.gene66342.t1 MTR_2g090730 57.692 78 29 1 81 158 27 100 4.90e-22 94.0
MS.gene66342.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 8.24e-18 85.9
MS.gene66342.t1 MTR_8g023140 23.181 371 257 14 86 433 149 514 8.31e-18 86.3
MS.gene66342.t1 MTR_5g033230 25.301 332 222 14 73 382 83 410 9.01e-18 85.9
MS.gene66342.t1 MTR_4g011820 22.917 336 222 12 105 431 75 382 1.40e-14 75.5
MS.gene66342.t1 MTR_8g061360 21.168 411 276 15 46 440 38 416 1.34e-11 66.6
MS.gene66342.t1 MTR_7g106830 19.637 331 243 10 114 436 151 466 6.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66342.t1 AT4G26200 75.638 431 102 3 12 439 16 446 0.0 665
MS.gene66342.t1 AT4G37770 60.563 426 164 2 14 435 4 429 0.0 557
MS.gene66342.t1 AT4G08040 60.798 426 166 1 14 438 2 427 0.0 554
MS.gene66342.t1 AT5G65800 58.837 430 173 2 13 438 3 432 0.0 545
MS.gene66342.t1 AT3G49700 58.333 432 176 2 13 440 3 434 0.0 542
MS.gene66342.t1 AT2G22810 58.333 432 175 3 12 438 2 433 0.0 538
MS.gene66342.t1 AT3G61510 58.531 422 171 2 14 434 11 429 0.0 536
MS.gene66342.t1 AT4G11280 58.670 421 173 1 14 433 15 435 0.0 530
MS.gene66342.t1 AT1G01480 54.524 431 192 3 14 440 12 442 2.17e-178 508
MS.gene66342.t1 AT1G01480 53.571 336 153 2 108 440 1 336 1.44e-136 397
MS.gene66342.t1 AT5G51690 43.396 424 237 2 12 433 67 489 2.31e-136 401
MS.gene66342.t1 AT1G62960 42.787 409 229 3 24 431 143 547 1.58e-127 380
MS.gene66342.t1 AT5G51690 46.064 343 184 1 89 431 23 364 3.86e-121 358
MS.gene66342.t1 AT1G72330 24.865 370 252 14 86 433 165 530 6.38e-20 92.8
MS.gene66342.t1 AT1G23310 26.259 278 185 10 91 352 103 376 8.34e-20 91.7
MS.gene66342.t1 AT1G23310 26.259 278 185 10 91 352 103 376 1.14e-19 91.7
MS.gene66342.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66342.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66342.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66342.t1 AT1G70580 25.540 278 187 10 91 352 103 376 6.23e-19 89.4
MS.gene66342.t1 AT1G72330 24.021 383 252 14 86 433 165 543 4.23e-18 87.0
MS.gene66342.t1 AT1G72330 25.573 262 181 8 86 336 165 423 1.10e-17 85.5
MS.gene66342.t1 AT1G17290 23.826 298 207 11 86 367 168 461 3.02e-17 84.3
MS.gene66342.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66342.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66342.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66342.t1 AT1G80360 23.678 397 252 16 43 431 28 381 9.89e-17 82.0
MS.gene66342.t1 AT2G24850 24.000 350 232 14 96 433 92 419 1.48e-15 79.0
MS.gene66342.t1 AT1G77670 24.458 323 214 11 121 437 141 439 1.63e-14 75.5
MS.gene66342.t1 AT4G23600 23.056 360 236 14 96 440 78 411 6.37e-14 73.9
MS.gene66342.t1 AT5G36160 22.096 353 234 13 96 433 85 411 1.36e-13 72.8
MS.gene66342.t1 AT2G20610 22.284 359 240 14 96 440 109 442 2.40e-12 68.9
MS.gene66342.t1 AT4G23600 23.263 331 215 13 125 440 1 307 9.10e-12 66.2
MS.gene66342.t1 AT2G20610 23.810 273 177 10 96 358 109 360 1.01e-11 67.0
MS.gene66342.t1 AT4G23600 23.264 288 188 10 96 370 78 345 3.11e-11 65.1

Find 80 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCGAACCCGGTTGGTTT+AGG 0.229798 6.4:-63142741 MS.gene66342:CDS
TTCCGAACCCGGTTGGTTTA+GGG 0.281999 6.4:-63142740 MS.gene66342:CDS
TTGTGTTTGTGAAGATATTA+AGG 0.307747 6.4:+63143032 None:intergenic
AGGTAAAACTCGACATTCTT+CGG 0.310933 6.4:+63143052 None:intergenic
TGTAACAACTTTATCGTTGT+AGG 0.335375 6.4:+63143080 None:intergenic
ATTTCTTACCCAGGATAGTA+TGG 0.344459 6.4:+63143928 None:intergenic
AGGTCAATAGAGAAAGATTA+AGG 0.358277 6.4:-63142957 MS.gene66342:CDS
GCTTTCCATCCAGCAAAGTA+TGG 0.363582 6.4:+63144506 None:intergenic
TATAGTCTTTCTAAAGATCT+TGG 0.364131 6.4:-63143135 MS.gene66342:CDS
CATGAAATAACTAACCCTTC+TGG 0.371377 6.4:-63144464 MS.gene66342:CDS
GAAAATTCTCCATACTTTGC+TGG 0.375504 6.4:-63144515 MS.gene66342:CDS
AAAAGCAATGGCAAGTTTCA+TGG 0.377867 6.4:-63144079 MS.gene66342:CDS
AACCCTAAACCAACCGGGTT+CGG 0.381536 6.4:+63142738 None:intergenic
CGTTGAATCGATATGCCTAA+TGG 0.381900 6.4:+63143321 None:intergenic
GGTAAAACTCGACATTCTTC+GGG 0.382775 6.4:+63143053 None:intergenic
GGGTTAGTTATTTCATGATA+AGG 0.383889 6.4:+63144470 None:intergenic
GATATGCCTAATGGATTTGA+TGG 0.385429 6.4:+63143330 None:intergenic
GGTTAGTTATTTCATGATAA+GGG 0.406672 6.4:+63144471 None:intergenic
GAGCAGGTGAATTGAAGCTT+TGG 0.427210 6.4:-63142819 MS.gene66342:CDS
ATTGAAGGTTTGAAAAGTGC+TGG 0.436332 6.4:-63142916 MS.gene66342:CDS
AAAACAAACCCTAAACCAAC+CGG 0.440582 6.4:+63142732 None:intergenic
ACTTTCATCCTTGCAAATCC+AGG 0.440957 6.4:-63143973 MS.gene66342:CDS
GATTTGATAGAGATTTAAGA+TGG 0.441356 6.4:-63143488 MS.gene66342:CDS
AGATATGAAATGATCATTGA+AGG 0.446464 6.4:-63142931 MS.gene66342:CDS
TCTTGTCATTGTTCCGAACC+CGG 0.453246 6.4:-63142751 MS.gene66342:CDS
CCCTTCTGGAGTTATACAAA+TGG 0.454742 6.4:-63144450 MS.gene66342:CDS
AGAGATTTAAGATGGAGAAC+TGG 0.456614 6.4:-63143480 MS.gene66342:CDS
GTTCCAACACCATACTATCC+TGG 0.457037 6.4:-63143937 MS.gene66342:CDS
AACAAGGACAGAAAGGATAC+AGG 0.467234 6.4:-63142649 MS.gene66342:CDS
TGGAGTTATACAAATGGGCT+TGG 0.477948 6.4:-63144444 MS.gene66342:CDS
TTTGAGCTATCACAATGGAT+TGG 0.480408 6.4:+63143444 None:intergenic
GATGAAAGTCAAGAGTTCAT+TGG 0.480725 6.4:+63143987 None:intergenic
TCTAAAGATCTTGGTCTACC+AGG 0.481569 6.4:-63143126 MS.gene66342:CDS
GCATTATTTCAAGACTATCA+TGG 0.482023 6.4:-63144117 MS.gene66342:CDS
CTTGTATTACTTTCCAACAT+TGG 0.485720 6.4:+63142841 None:intergenic
GAGGCATCAACATGGGGAAA+AGG 0.488094 6.4:-63144162 MS.gene66342:CDS
CCTTCTGGAGTTATACAAAT+GGG 0.492469 6.4:-63144449 MS.gene66342:CDS
ATTCTCCATACTTTGCTGGA+TGG 0.499110 6.4:-63144511 MS.gene66342:CDS
GTCATTGTTCCGAACCCGGT+TGG 0.507280 6.4:-63142747 MS.gene66342:CDS
CATGAATAACAAGGACAGAA+AGG 0.509122 6.4:-63142656 MS.gene66342:CDS
TTGGAAAGTAATACAAGAGC+AGG 0.510180 6.4:-63142835 MS.gene66342:CDS
GAATGCTTGAAAGGTAATGC+AGG 0.512496 6.4:-63142889 MS.gene66342:CDS
GAAAGAATTGTCCTCACTGC+TGG 0.516086 6.4:-63144021 MS.gene66342:CDS
TACCCAGGATAGTATGGTGT+TGG 0.517904 6.4:+63143934 None:intergenic
CCCATTTGTATAACTCCAGA+AGG 0.531454 6.4:+63144449 None:intergenic
TTCCAACACCATACTATCCT+GGG 0.532294 6.4:-63143936 MS.gene66342:intron
ATTGCAACTTCTGAAACTCA+TGG 0.536344 6.4:-63144539 MS.gene66342:CDS
GATGAATATGAGTCCAATGT+TGG 0.544594 6.4:-63142854 MS.gene66342:CDS
TTTCACTGATAATTACATCA+AGG 0.546541 6.4:-63142977 MS.gene66342:CDS
GCCTAATGGATTTGATGGGT+TGG 0.548657 6.4:+63143335 None:intergenic
GGAACAAGTAAAGCATCTCC+TGG 0.548786 6.4:+63143955 None:intergenic
TTGACGAGATTCAAGAATCT+CGG 0.556243 6.4:+63143188 None:intergenic
TGTCTCAGACGAAATCTACT+CGG 0.564955 6.4:-63143247 MS.gene66342:CDS
GGTCTACCAGGTTTCAGAGT+CGG 0.574668 6.4:-63143114 MS.gene66342:CDS
GTTAAGCTTAATATTTCACC+AGG 0.577298 6.4:-63142778 MS.gene66342:CDS
TAAATCATTCAGAAAAGCAA+TGG 0.583118 6.4:-63144091 MS.gene66342:CDS
GCTGGAATTGAATGCTTGAA+AGG 0.592728 6.4:-63142898 MS.gene66342:CDS
AACACTCAGAGGCATCAACA+TGG 0.595204 6.4:-63144170 MS.gene66342:CDS
ACCAACCCATCAAATCCATT+AGG 0.597602 6.4:-63143336 MS.gene66342:CDS
ATTGTTCCGACTCTGAAACC+TGG 0.601886 6.4:+63143108 None:intergenic
ACACTCAGAGGCATCAACAT+GGG 0.602352 6.4:-63144169 MS.gene66342:CDS
GTCTCAGACGAAATCTACTC+GGG 0.602761 6.4:-63143246 MS.gene66342:CDS
GCATCAACATGGGGAAAAGG+TGG 0.616595 6.4:-63144159 MS.gene66342:CDS
ACAAGGACAGAAAGGATACA+GGG 0.626149 6.4:-63142648 MS.gene66342:CDS
CACTCAGAGGCATCAACATG+GGG 0.626693 6.4:-63144168 MS.gene66342:CDS
AGTAGATTTCGTCTGAGACA+AGG 0.630503 6.4:+63143250 None:intergenic
AAGCATCTCCTGGATTTGCA+AGG 0.633214 6.4:+63143965 None:intergenic
GATTCAACGTTCAGTTCTCG+AGG 0.639409 6.4:-63143307 MS.gene66342:CDS
CCATTTGTATAACTCCAGAA+GGG 0.643005 6.4:+63144450 None:intergenic
ATATGCCTAATGGATTTGAT+GGG 0.644532 6.4:+63143331 None:intergenic
AATTGTTTGAGCTATCACAA+TGG 0.655921 6.4:+63143439 None:intergenic
ATATTTGAAAGTTCAACACA+AGG 0.665047 6.4:+63144560 None:intergenic
ACGTGATTTCATGAATAACA+AGG 0.666747 6.4:-63142665 MS.gene66342:CDS
AAACAAACCCTAAACCAACC+GGG 0.670128 6.4:+63142733 None:intergenic
TATTTGAAAGTTCAACACAA+GGG 0.671806 6.4:+63144561 None:intergenic
GAACAATGACAAGAACAACC+TGG 0.683485 6.4:+63142760 None:intergenic
CAGCAGTAGCACCAGCAGTG+AGG 0.696586 6.4:+63144010 None:intergenic
ATACTTGGAAGAACACTCAG+AGG 0.699650 6.4:-63144181 MS.gene66342:CDS
AATGCTTGAAAGGTAATGCA+GGG 0.724648 6.4:-63142888 MS.gene66342:CDS
ATCTCGTCAATACAAAGACG+CGG 0.725438 6.4:-63143175 MS.gene66342:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATAAAAATTTTGGTAT+AGG + chr6.4:63143392-63143411 None:intergenic 10.0%
!!! TATTTCTAAATTCGAAAATA+TGG - chr6.4:63143568-63143587 MS.gene66342:intron 15.0%
!!! TTTTTTGTTTGAAATTCTTT+AGG - chr6.4:63143003-63143022 MS.gene66342:CDS 15.0%
!! GACAATATAAGTACAATAAT+TGG - chr6.4:63143682-63143701 MS.gene66342:intron 20.0%
!! TCTCTTACCATAATTAATAA+TGG - chr6.4:63143360-63143379 MS.gene66342:CDS 20.0%
!!! ATAAAAATTTTGGTATAGGA+AGG + chr6.4:63143388-63143407 None:intergenic 20.0%
!!! GATATATGTACTTTAAAGAA+AGG - chr6.4:63142915-63142934 MS.gene66342:CDS 20.0%
!!! TATTTGCTTAAATGCATTTT+TGG - chr6.4:63143509-63143528 MS.gene66342:intron 20.0%
! AGATATGAAATGATCATTGA+AGG - chr6.4:63144298-63144317 MS.gene66342:intron 25.0%
! AGTTTCATGGAAAAAATTAG+AGG - chr6.4:63143163-63143182 MS.gene66342:CDS 25.0%
! ATATTTGAAAGTTCAACACA+AGG + chr6.4:63142672-63142691 None:intergenic 25.0%
! GAAGGTTCCATTATTAATTA+TGG + chr6.4:63143370-63143389 None:intergenic 25.0%
! GATTTGATAGAGATTTAAGA+TGG - chr6.4:63143741-63143760 MS.gene66342:intron 25.0%
! TAAATCATTCAGAAAAGCAA+TGG - chr6.4:63143138-63143157 MS.gene66342:CDS 25.0%
! TATTTGAAAGTTCAACACAA+GGG + chr6.4:63142671-63142690 None:intergenic 25.0%
! TTGTGTTTGTGAAGATATTA+AGG + chr6.4:63144200-63144219 None:intergenic 25.0%
! TTTCACTGATAATTACATCA+AGG - chr6.4:63144252-63144271 MS.gene66342:intron 25.0%
!! GGTTAGTTATTTCATGATAA+GGG + chr6.4:63142761-63142780 None:intergenic 25.0%
!! TATAGTCTTTCTAAAGATCT+TGG - chr6.4:63144094-63144113 MS.gene66342:CDS 25.0%
!!! CAAACTTTTTTTTTTTCCCT+TGG - chr6.4:63143426-63143445 MS.gene66342:CDS 25.0%
!!! TGATTTGCTTGAAAAATACT+TGG - chr6.4:63143033-63143052 MS.gene66342:CDS 25.0%
AAAGAGATAATTTCTTACCC+AGG + chr6.4:63143313-63143332 None:intergenic 30.0%
AATTGTTTGAGCTATCACAA+TGG + chr6.4:63143793-63143812 None:intergenic 30.0%
ACGTGATTTCATGAATAACA+AGG - chr6.4:63144564-63144583 MS.gene66342:CDS 30.0%
AGGTCAATAGAGAAAGATTA+AGG - chr6.4:63144272-63144291 MS.gene66342:intron 30.0%
CTTGTATTACTTTCCAACAT+TGG + chr6.4:63144391-63144410 None:intergenic 30.0%
GCATTATTTCAAGACTATCA+TGG - chr6.4:63143112-63143131 MS.gene66342:CDS 30.0%
GTTAAGCTTAATATTTCACC+AGG - chr6.4:63144451-63144470 MS.gene66342:CDS 30.0%
TGTAACAACTTTATCGTTGT+AGG + chr6.4:63144152-63144171 None:intergenic 30.0%
! ATGATAAGGGTTTTCATCAT+AGG + chr6.4:63142748-63142767 None:intergenic 30.0%
! TGCTTTTACTTTCATGTTCA+TGG + chr6.4:63143864-63143883 None:intergenic 30.0%
!! ATATGCCTAATGGATTTGAT+GGG + chr6.4:63143901-63143920 None:intergenic 30.0%
!! GGGTTAGTTATTTCATGATA+AGG + chr6.4:63142762-63142781 None:intergenic 30.0%
AAAACAAACCCTAAACCAAC+CGG + chr6.4:63144500-63144519 None:intergenic 35.0%
AAAAGCAATGGCAAGTTTCA+TGG - chr6.4:63143150-63143169 MS.gene66342:CDS 35.0%
AATGCTTGAAAGGTAATGCA+GGG - chr6.4:63144341-63144360 MS.gene66342:intron 35.0%
AGGTAAAACTCGACATTCTT+CGG + chr6.4:63144180-63144199 None:intergenic 35.0%
ATTTCTTACCCAGGATAGTA+TGG + chr6.4:63143304-63143323 None:intergenic 35.0%
CATGAAATAACTAACCCTTC+TGG - chr6.4:63142765-63142784 MS.gene66342:CDS 35.0%
CATGAATAACAAGGACAGAA+AGG - chr6.4:63144573-63144592 MS.gene66342:CDS 35.0%
CCATTTGTATAACTCCAGAA+GGG + chr6.4:63142782-63142801 None:intergenic 35.0%
GAAAATTCTCCATACTTTGC+TGG - chr6.4:63142714-63142733 MS.gene66342:CDS 35.0%
GATGAAAGTCAAGAGTTCAT+TGG + chr6.4:63143245-63143264 None:intergenic 35.0%
GATGAATATGAGTCCAATGT+TGG - chr6.4:63144375-63144394 MS.gene66342:intron 35.0%
TAAATGCTTGCAAGTTCCAA+GGG + chr6.4:63143445-63143464 None:intergenic 35.0%
TTAAATGCTTGCAAGTTCCA+AGG + chr6.4:63143446-63143465 None:intergenic 35.0%
TTGACGAGATTCAAGAATCT+CGG + chr6.4:63144044-63144063 None:intergenic 35.0%
TTGGAAAGTAATACAAGAGC+AGG - chr6.4:63144394-63144413 MS.gene66342:intron 35.0%
TTTGAGCTATCACAATGGAT+TGG + chr6.4:63143788-63143807 None:intergenic 35.0%
! AGAGATTTAAGATGGAGAAC+TGG - chr6.4:63143749-63143768 MS.gene66342:intron 35.0%
! ATTGAAGGTTTGAAAAGTGC+TGG - chr6.4:63144313-63144332 MS.gene66342:intron 35.0%
! TTTTCTTATATCGTTGACCC+AGG + chr6.4:63142862-63142881 None:intergenic 35.0%
!! ATTGCAACTTCTGAAACTCA+TGG - chr6.4:63142690-63142709 MS.gene66342:CDS 35.0%
!! CCTTCTGGAGTTATACAAAT+GGG - chr6.4:63142780-63142799 MS.gene66342:CDS 35.0%
!! GATATGCCTAATGGATTTGA+TGG + chr6.4:63143902-63143921 None:intergenic 35.0%
!! TCTTTTGAGTGCAAGTTGAA+GGG + chr6.4:63144542-63144561 None:intergenic 35.0%
!! TTCTTTTGAGTGCAAGTTGA+AGG + chr6.4:63144543-63144562 None:intergenic 35.0%
AAACAAACCCTAAACCAACC+GGG + chr6.4:63144499-63144518 None:intergenic 40.0%
AACAAGGACAGAAAGGATAC+AGG - chr6.4:63144580-63144599 MS.gene66342:CDS 40.0%
ACAAGGACAGAAAGGATACA+GGG - chr6.4:63144581-63144600 MS.gene66342:CDS 40.0%
ACCAACCCATCAAATCCATT+AGG - chr6.4:63143893-63143912 MS.gene66342:intron 40.0%
ACTTTCATCCTTGCAAATCC+AGG - chr6.4:63143256-63143275 MS.gene66342:CDS 40.0%
ATACTTGGAAGAACACTCAG+AGG - chr6.4:63143048-63143067 MS.gene66342:CDS 40.0%
ATCTCGTCAATACAAAGACG+CGG - chr6.4:63144054-63144073 MS.gene66342:CDS 40.0%
ATTCTCCATACTTTGCTGGA+TGG - chr6.4:63142718-63142737 MS.gene66342:CDS 40.0%
CCCATTTGTATAACTCCAGA+AGG + chr6.4:63142783-63142802 None:intergenic 40.0%
CGTTGAATCGATATGCCTAA+TGG + chr6.4:63143911-63143930 None:intergenic 40.0%
GAACAATGACAAGAACAACC+TGG + chr6.4:63144472-63144491 None:intergenic 40.0%
GAATGCTTGAAAGGTAATGC+AGG - chr6.4:63144340-63144359 MS.gene66342:intron 40.0%
GCTGGAATTGAATGCTTGAA+AGG - chr6.4:63144331-63144350 MS.gene66342:intron 40.0%
GGTAAAACTCGACATTCTTC+GGG + chr6.4:63144179-63144198 None:intergenic 40.0%
TCTAAAGATCTTGGTCTACC+AGG - chr6.4:63144103-63144122 MS.gene66342:CDS 40.0%
TGTCTCAGACGAAATCTACT+CGG - chr6.4:63143982-63144001 MS.gene66342:CDS 40.0%
TTCCAACACCATACTATCCT+GGG - chr6.4:63143293-63143312 MS.gene66342:CDS 40.0%
! AGTAGATTTCGTCTGAGACA+AGG + chr6.4:63143982-63144001 None:intergenic 40.0%
! TGGAGTTATACAAATGGGCT+TGG - chr6.4:63142785-63142804 MS.gene66342:CDS 40.0%
!! CCCTTCTGGAGTTATACAAA+TGG - chr6.4:63142779-63142798 MS.gene66342:CDS 40.0%
!! GTAATGCAGGGTTGTTTTGT+TGG - chr6.4:63144353-63144372 MS.gene66342:intron 40.0%
AAGCATCTCCTGGATTTGCA+AGG + chr6.4:63143267-63143286 None:intergenic 45.0%
CTTATATCGTTGACCCAGGT+CGG + chr6.4:63142858-63142877 None:intergenic 45.0%
GAAAGAATTGTCCTCACTGC+TGG - chr6.4:63143208-63143227 MS.gene66342:CDS 45.0%
GAGCAGGTGAATTGAAGCTT+TGG - chr6.4:63144410-63144429 MS.gene66342:intron 45.0%
GATTCAACGTTCAGTTCTCG+AGG - chr6.4:63143922-63143941 MS.gene66342:intron 45.0%
GCTTTCCATCCAGCAAAGTA+TGG + chr6.4:63142726-63142745 None:intergenic 45.0%
GTCTCAGACGAAATCTACTC+GGG - chr6.4:63143983-63144002 MS.gene66342:CDS 45.0%
GTTCCAACACCATACTATCC+TGG - chr6.4:63143292-63143311 MS.gene66342:CDS 45.0%
TACCCAGGATAGTATGGTGT+TGG + chr6.4:63143298-63143317 None:intergenic 45.0%
TCTTGTCATTGTTCCGAACC+CGG - chr6.4:63144478-63144497 MS.gene66342:CDS 45.0%
! GGAACAAGTAAAGCATCTCC+TGG + chr6.4:63143277-63143296 None:intergenic 45.0%
!! AACACTCAGAGGCATCAACA+TGG - chr6.4:63143059-63143078 MS.gene66342:CDS 45.0%
!! ACACTCAGAGGCATCAACAT+GGG - chr6.4:63143060-63143079 MS.gene66342:CDS 45.0%
!! ATTGTTCCGACTCTGAAACC+TGG + chr6.4:63144124-63144143 None:intergenic 45.0%
!! GCCTAATGGATTTGATGGGT+TGG + chr6.4:63143897-63143916 None:intergenic 45.0%
!!! AAACTATATTATAAAAATTT+TGG + chr6.4:63143398-63143417 None:intergenic 5.0%
AACCCTAAACCAACCGGGTT+CGG + chr6.4:63144494-63144513 None:intergenic 50.0%
GCATCAACATGGGGAAAAGG+TGG - chr6.4:63143070-63143089 MS.gene66342:CDS 50.0%
GGTCTACCAGGTTTCAGAGT+CGG - chr6.4:63144115-63144134 MS.gene66342:CDS 50.0%
! ACGTTTTCAGTGTCCGACCT+GGG - chr6.4:63142842-63142861 MS.gene66342:CDS 50.0%
! GAGGCATCAACATGGGGAAA+AGG - chr6.4:63143067-63143086 MS.gene66342:CDS 50.0%
!! CACTCAGAGGCATCAACATG+GGG - chr6.4:63143061-63143080 MS.gene66342:CDS 50.0%
!! TTCCGAACCCGGTTGGTTTA+GGG - chr6.4:63144489-63144508 MS.gene66342:CDS 50.0%
GTCATTGTTCCGAACCCGGT+TGG - chr6.4:63144482-63144501 MS.gene66342:CDS 55.0%
! GACGTTTTCAGTGTCCGACC+TGG - chr6.4:63142841-63142860 MS.gene66342:CDS 55.0%
!! GTTCCGAACCCGGTTGGTTT+AGG - chr6.4:63144488-63144507 MS.gene66342:CDS 55.0%
CAGCAGTAGCACCAGCAGTG+AGG + chr6.4:63143222-63143241 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 63142642 63144609 63142642 ID=MS.gene66342
chr6.4 mRNA 63142642 63144609 63142642 ID=MS.gene66342.t1;Parent=MS.gene66342
chr6.4 exon 63144433 63144609 63144433 ID=MS.gene66342.t1.exon1;Parent=MS.gene66342.t1
chr6.4 CDS 63144433 63144609 63144433 ID=cds.MS.gene66342.t1;Parent=MS.gene66342.t1
chr6.4 exon 63143937 63144226 63143937 ID=MS.gene66342.t1.exon2;Parent=MS.gene66342.t1
chr6.4 CDS 63143937 63144226 63143937 ID=cds.MS.gene66342.t1;Parent=MS.gene66342.t1
chr6.4 exon 63142642 63143509 63142642 ID=MS.gene66342.t1.exon3;Parent=MS.gene66342.t1
chr6.4 CDS 63142642 63143509 63142642 ID=cds.MS.gene66342.t1;Parent=MS.gene66342.t1
Gene Sequence

>MS.gene66342

ATGGGTATTGAGATTGAACAAGAACACCCTTGTGTTGAACTTTCAAATATTGCAACTTCTGAAACTCATGGAGAAAATTCTCCATACTTTGCTGGATGGAAAGCCTATGATGAAAACCCTTATCATGAAATAACTAACCCTTCTGGAGTTATACAAATGGGCTTGGCAGAAAATCAAGTAAGTGTATAAATATATCGAAGACGTTTTCAGTGTCCGACCTGGGTCAACGATATAAGAAAAAAAAGTACATGTATCTGATATAAATTTTGATCAGATATATGTACTTTAAAGAAAGGAAAAAAATGTGACACTTAATTATTTTCATTAATTTCTAAGCTTATATTTAACTAGTTAACTAATATTTTTTGTTTGAAATTCTTTAGGTATCATTTGATTTGCTTGAAAAATACTTGGAAGAACACTCAGAGGCATCAACATGGGGAAAAGGTGGTTCAAGTTTTAGAGATAATGCATTATTTCAAGACTATCATGGTCTTAAATCATTCAGAAAAGCAATGGCAAGTTTCATGGAAAAAATTAGAGGAAATAAAGCAAAATTTGATTATGAAAGAATTGTCCTCACTGCTGGTGCTACTGCTGCCAATGAACTCTTGACTTTCATCCTTGCAAATCCAGGAGATGCTTTACTTGTTCCAACACCATACTATCCTGGGTAAGAAATTATCTCTTTTCACACTTCAATACATTTTTATATTTTTCTCTTACCATAATTAATAATGGAACCTTCCTATACCAAAATTTTTATAATATAGTTTCAAAATGTCAAACTTTTTTTTTTTCCCTTGGAACTTGCAAGCATTTAACTATTTGCTTTATGAGTATAATAAAAATATATTCTATATAATATATTTGCTTAAATGCATTTTTGGTCGTCTATTTTTACTAATTCATGATTTTAGCTCGTGTATTTCTAAATTCGAAAATATGGTCTTCATGTTTTCAAATTTAATGAGCACTAAAATCGTGAATCTATTAAAAAAATGATCAAACGAGTAATCATCTAAGCATAAAATTCGCGCGACAATATAAGTACAATAATTGGAAAATTAAACTAATGTATATATTTTTTGATTTTGCAGATTTGATAGAGATTTAAGATGGAGAACTGGTGTAAACATAGTTCCAATCCATTGTGATAGCTCAAACAATTTTCAAATCACACTTGAAGCATTAGAAACTGCATACAAAAATGCAGAATCCATGAACATGAAAGTAAAAGCAGTACTTATCACCAACCCATCAAATCCATTAGGCATATCGATTCAACGTTCAGTTCTCGAGGACATTCTCAATTTCGTGACTCGCAAGAACATACACCTTGTCTCAGACGAAATCTACTCGGGCTCAGTTTTCTCTTCACATGAATTCATAAGCATAGCCGAGATTCTTGAATCTCGTCAATACAAAGACGCGGAAAGATGTCACATTGTTTATAGTCTTTCTAAAGATCTTGGTCTACCAGGTTTCAGAGTCGGAACAATTTATTCCTACAACGATAAAGTTGTTACAACAGCCCGAAGAATGTCGAGTTTTACCTTAATATCTTCACAAACACAACATCTTTTAGCATCAATGTTGTCAGATGAAAGTTTCACTGATAATTACATCAAGGTCAATAGAGAAAGATTAAGGAAAAGATATGAAATGATCATTGAAGGTTTGAAAAGTGCTGGAATTGAATGCTTGAAAGGTAATGCAGGGTTGTTTTGTTGGATGAATATGAGTCCAATGTTGGAAAGTAATACAAGAGCAGGTGAATTGAAGCTTTGGAATGAGATTTTGAATGAAGTTAAGCTTAATATTTCACCAGGTTGTTCTTGTCATTGTTCCGAACCCGGTTGGTTTAGGGTTTGTTTTGCAAATATGAGTGAAGAAACCCTTCAACTTGCACTCAAAAGAATACGTGATTTCATGAATAACAAGGACAGAAAGGATACAGGGATATAA
Protein sequence

>MS.gene66342.t1

MGIEIEQEHPCVELSNIATSETHGENSPYFAGWKAYDENPYHEITNPSGVIQMGLAENQVSFDLLEKYLEEHSEASTWGKGGSSFRDNALFQDYHGLKSFRKAMASFMEKIRGNKAKFDYERIVLTAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVNIVPIHCDSSNNFQITLEALETAYKNAESMNMKVKAVLITNPSNPLGISIQRSVLEDILNFVTRKNIHLVSDEIYSGSVFSSHEFISIAEILESRQYKDAERCHIVYSLSKDLGLPGFRVGTIYSYNDKVVTTARRMSSFTLISSQTQHLLASMLSDESFTDNYIKVNRERLRKRYEMIIEGLKSAGIECLKGNAGLFCWMNMSPMLESNTRAGELKLWNEILNEVKLNISPGCSCHCSEPGWFRVCFANMSEETLQLALKRIRDFMNNKDRKDTGI