AlfalfaGEDB | MS.gene63621

M. sativa cultivar XinJiangDaYe / MS.gene63621

KEGG: K18195
GO: -
PFAM: PF06045.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63621.t1	RYR42136.1	94.5	55	3	0	1	55	95	149	3.20E-24	120.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63621.t1	A0A445BU56	94.5	55	3	0	1	55	95	149	2.3e-24	120.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene63621	0.824706	6.98E-54	-1.69E-46
MS.gene049825	MS.gene63621	0.806129	9.56E-50	-1.69E-46
MS.gene051332	MS.gene63621	0.809448	1.88E-50	-1.69E-46
MS.gene051902	MS.gene63621	0.806837	6.77E-50	-1.69E-46
MS.gene052108	MS.gene63621	0.811473	6.88E-51	-1.69E-46
MS.gene052582	MS.gene63621	0.808138	3.59E-50	-1.69E-46
MS.gene052584	MS.gene63621	0.818957	1.49E-52	-1.69E-46
MS.gene052773	MS.gene63621	0.805999	1.02E-49	-1.69E-46
MS.gene053226	MS.gene63621	0.802945	4.41E-49	-1.69E-46
MS.gene056050	MS.gene63621	0.804113	2.52E-49	-1.69E-46
MS.gene056776	MS.gene63621	0.807907	4.02E-50	-1.69E-46
MS.gene05689	MS.gene63621	0.801369	9.30E-49	-1.69E-46
MS.gene057102	MS.gene63621	0.805017	1.64E-49	-1.69E-46
MS.gene057208	MS.gene63621	0.806141	9.50E-50	-1.69E-46
MS.gene057598	MS.gene63621	0.800518	1.39E-48	-1.69E-46
MS.gene057893	MS.gene63621	-0.815079	1.11E-51	-1.69E-46
MS.gene057919	MS.gene63621	0.814961	1.18E-51	-1.69E-46
MS.gene058625	MS.gene63621	0.813022	3.16E-51	-1.69E-46
MS.gene059625	MS.gene63621	0.819917	9.03E-53	-1.69E-46
MS.gene060365	MS.gene63621	0.823425	1.40E-53	-1.69E-46
MS.gene060634	MS.gene63621	-0.818793	1.63E-52	-1.69E-46
MS.gene060935	MS.gene63621	0.804974	1.67E-49	-1.69E-46
MS.gene060937	MS.gene63621	0.815188	1.05E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63621.t1	MTR_3g111420	96.491	57	1	1	2	57	114	170	3.56e-30	110
MS.gene63621.t1	MTR_3g111410	77.193	57	12	1	2	57	99	155	1.90e-23	91.3
MS.gene63621.t1	MTR_3g111380	70.175	57	16	1	2	57	107	163	2.43e-21	85.1
MS.gene63621.t1	MTR_3g111380	70.175	57	16	1	2	57	28	84	2.58e-21	85.1
MS.gene63621.t1	MTR_3g111380	70.175	57	16	1	2	57	19	75	2.84e-21	84.7
MS.gene63621.t1	MTR_5g066770	64.912	57	19	1	2	57	99	155	6.82e-20	80.9
MS.gene63621.t1	MTR_8g068110	48.148	54	28	0	2	55	154	207	7.20e-12	58.2
MS.gene63621.t1	MTR_8g068110	48.148	54	28	0	2	55	145	198	7.63e-12	58.2
MS.gene63621.t1	MTR_8g068120	46.552	58	30	1	1	57	157	214	3.15e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63621.t1	AT1G09910	78.947	57	11	1	2	57	139	195	8.60e-25	95.1
MS.gene63621.t1	AT1G09910	78.947	57	11	1	2	57	96	152	1.10e-24	94.7
MS.gene63621.t1	AT1G09910	78.947	57	11	1	2	57	96	152	1.10e-24	94.7
MS.gene63621.t1	AT1G09890	69.643	56	17	0	2	57	96	151	1.36e-22	88.6
MS.gene63621.t1	AT1G09890	72.222	54	15	0	2	55	80	133	1.42e-22	88.6
MS.gene63621.t1	AT1G09890	72.222	54	15	0	2	55	96	149	1.47e-22	88.6
MS.gene63621.t1	AT1G09890	72.222	54	15	0	2	55	96	149	1.84e-22	88.2
MS.gene63621.t1	AT1G09890	66.667	54	13	1	2	55	96	144	1.60e-17	74.3
MS.gene63621.t1	AT4G24430	54.386	57	25	1	2	57	100	156	3.35e-16	70.5
MS.gene63621.t1	AT2G22620	55.357	56	25	0	2	57	141	196	2.12e-15	68.2
MS.gene63621.t1	AT2G22620	55.357	56	25	0	2	57	141	196	2.41e-15	68.2
MS.gene63621.t1	AT2G22620	55.357	56	25	0	2	57	141	196	2.41e-15	68.2
MS.gene63621.t1	AT2G22620	55.357	56	25	0	2	57	141	196	2.97e-15	67.8
MS.gene63621.t1	AT1G09880	57.447	47	20	0	11	57	174	220	5.48e-14	64.3
MS.gene63621.t1	AT1G09880	57.447	47	20	0	11	57	160	206	5.64e-14	64.3
MS.gene63621.t1	AT1G09880	57.447	47	20	0	11	57	128	174	5.86e-14	64.3
MS.gene63621.t1	AT1G09880	57.447	47	20	0	11	57	94	140	6.45e-14	63.9
MS.gene63621.t1	AT4G37950	52.941	51	24	0	5	55	141	191	2.01e-13	62.8
MS.gene63621.t1	AT4G37950	52.941	51	24	0	5	55	141	191	2.01e-13	62.8
MS.gene63621.t1	AT4G38030	53.061	49	23	0	7	55	135	183	5.93e-13	61.2

Find 11 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATCAGGCTTCTACTCTTA+TGG	0.240846	3.2:+23827594	MS.gene63621:CDS
GTTTCACTAATATCAAAATC+TGG	0.370567	3.2:-23827642	None:intergenic
AATATCAAAATCTGGCCATC+CGG	0.419141	3.2:-23827634	None:intergenic
TCCCCTTAAATATTGACAAA+AGG	0.433210	3.2:+23827474	MS.gene63621:CDS
ATGTGGGATCCTTCTCTTGA+GGG	0.478112	3.2:+23827443	MS.gene63621:CDS
GGAATTTATGAGCACTTACC+CGG	0.490203	3.2:+23827615	MS.gene63621:CDS
ATATCAAAATCTGGCCATCC+GGG	0.534719	3.2:-23827633	None:intergenic
ACAAACTTTCCCTCAAGAGA+AGG	0.598936	3.2:-23827452	None:intergenic
TTTATGAGCACTTACCCGGA+TGG	0.612291	3.2:+23827619	MS.gene63621:CDS
AATGTGGGATCCTTCTCTTG+AGG	0.629331	3.2:+23827442	None:intergenic
AGTAGAAGCCTGATGAACCA+CGG	0.701660	3.2:-23827587	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATATTGACAAAAGGTAAT+TGG	+	chr3.2:23827481-23827500	MS.gene63621:intron	20.0%
!!	ATTGGATTAATATTTGTGAA+TGG	+	chr3.2:23827542-23827561	MS.gene63621:intron	20.0%
!!	TTCACAAATATTAATCCAAT+AGG	-	chr3.2:23827542-23827561	None:intergenic	20.0%
!!!	ATTTTGATATTAGTGAAACA+AGG	+	chr3.2:23827646-23827665	MS.gene63621:CDS	20.0%
!!!	TACCTTTTGTCAATATTTAA+GGG	-	chr3.2:23827479-23827498	None:intergenic	20.0%
!!!	TTACCTTTTGTCAATATTTA+AGG	-	chr3.2:23827480-23827499	None:intergenic	20.0%
!	GTTTCACTAATATCAAAATC+TGG	-	chr3.2:23827645-23827664	None:intergenic	25.0%
!!	ACCTTTTGTCAATATTTAAG+GGG	-	chr3.2:23827478-23827497	None:intergenic	25.0%
	TCCCCTTAAATATTGACAAA+AGG	+	chr3.2:23827474-23827493	MS.gene63621:CDS	30.0%
!!	TGAATAATGCGTTTTTGAAG+TGG	+	chr3.2:23827514-23827533	MS.gene63621:intron	30.0%
	AATATCAAAATCTGGCCATC+CGG	-	chr3.2:23827637-23827656	None:intergenic	35.0%
	GGAGCAAAATGAATCTGAAA+TGG	-	chr3.2:23827569-23827588	None:intergenic	35.0%
!!!	GTTTTTGAAGTGGTACCTAT+TGG	+	chr3.2:23827524-23827543	MS.gene63621:intron	35.0%
	ACAAACTTTCCCTCAAGAGA+AGG	-	chr3.2:23827455-23827474	None:intergenic	40.0%
	ATATCAAAATCTGGCCATCC+GGG	-	chr3.2:23827636-23827655	None:intergenic	40.0%
	GGAATTTATGAGCACTTACC+CGG	+	chr3.2:23827615-23827634	MS.gene63621:CDS	40.0%
	TCATCAGGCTTCTACTCTTA+TGG	+	chr3.2:23827594-23827613	MS.gene63621:CDS	40.0%
!	TTCAGATTCATTTTGCTCCG+TGG	+	chr3.2:23827570-23827589	MS.gene63621:intron	40.0%
	AGTAGAAGCCTGATGAACCA+CGG	-	chr3.2:23827590-23827609	None:intergenic	45.0%
	ATGTGGGATCCTTCTCTTGA+GGG	+	chr3.2:23827443-23827462	MS.gene63621:CDS	45.0%
	TTTATGAGCACTTACCCGGA+TGG	+	chr3.2:23827619-23827638	MS.gene63621:CDS	45.0%
!	ATTTTGCTCCGTGGTTCATC+AGG	+	chr3.2:23827579-23827598	MS.gene63621:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	23827443	23827695	23827443	ID=MS.gene63621
chr3.2	mRNA	23827443	23827695	23827443	ID=MS.gene63621.t1;Parent=MS.gene63621
chr3.2	exon	23827443	23827495	23827443	ID=MS.gene63621.t1.exon1;Parent=MS.gene63621.t1
chr3.2	CDS	23827443	23827495	23827443	ID=cds.MS.gene63621.t1;Parent=MS.gene63621.t1
chr3.2	exon	23827575	23827695	23827575	ID=MS.gene63621.t1.exon2;Parent=MS.gene63621.t1
chr3.2	CDS	23827575	23827695	23827575	ID=cds.MS.gene63621.t1;Parent=MS.gene63621.t1

Gene Sequence	>MS.gene63621 ATGTGGGATCCTTCTCTTGAGGGAAAGTTTGTCCCCTTAAATATTGACAAAAGGTAATTGGTCAAATTTTGTGAATAATGCGTTTTTGAAGTGGTACCTATTGGATTAATATTTGTGAATGGACCATTTCAGATTCATTTTGCTCCGTGGTTCATCAGGCTTCTACTCTTATGGAATTTATGAGCACTTACCCGGATGGCCAGATTTTGATATTAGTGAAACAAGGATCACTTTCAAGCTTAAAGACAAGTAA
Protein sequence	>MS.gene63621.t1 MWDPSLEGKFVPLNIDKRFILLRGSSGFYSYGIYEHLPGWPDFDISETRITFKLKDK