Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65617.t1 | XP_013445938.1 | 95.6 | 342 | 15 | 0 | 1 | 342 | 1 | 342 | 6.10E-187 | 663.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65617.t1 | Q9ZTR0 | 92.4 | 341 | 23 | 2 | 3 | 341 | 2 | 341 | 6.6e-182 | 637.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65617.t1 | A0A072U2G1 | 95.6 | 342 | 15 | 0 | 1 | 342 | 1 | 342 | 4.4e-187 | 663.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene65617 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene65617 | 0.819526 | 1.11E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene65617 | 0.814675 | 1.37E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene65617 | 0.818059 | 2.39E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene65617 | 0.809942 | 1.47E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene65617 | 0.801758 | 7.74E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene65617 | 0.804146 | 2.48E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene65617 | 0.814839 | 1.26E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene65617 | 0.828958 | 6.73E-55 | -1.69E-46 |
| MS.gene057448 | MS.gene65617 | 0.801232 | 9.92E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene65617 | 0.800901 | 1.16E-48 | -1.69E-46 |
| MS.gene057754 | MS.gene65617 | 0.846241 | 2.46E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene65617 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene060084 | MS.gene65617 | 0.833409 | 5.42E-56 | -1.69E-46 |
| MS.gene060495 | MS.gene65617 | 0.833094 | 6.50E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene65617 | 0.805467 | 1.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene65617 | MS.gene051552 | PPI |
| MS.gene65617 | MS.gene32768 | PPI |
| MS.gene65617 | MS.gene058418 | PPI |
| MS.gene65617 | MS.gene014976 | PPI |
| MS.gene65617 | MS.gene016160 | PPI |
| MS.gene42684 | MS.gene65617 | PPI |
| MS.gene65617 | MS.gene42624 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65617.t1 | MTR_8g063940 | 97.953 | 342 | 7 | 0 | 1 | 342 | 1 | 342 | 0.0 | 694 |
| MS.gene65617.t1 | MTR_5g034470 | 81.897 | 348 | 49 | 3 | 2 | 342 | 3 | 343 | 0.0 | 586 |
| MS.gene65617.t1 | MTR_5g034470 | 79.765 | 341 | 55 | 3 | 2 | 335 | 3 | 336 | 0.0 | 555 |
| MS.gene65617.t1 | MTR_3g091090 | 66.019 | 309 | 90 | 2 | 44 | 337 | 45 | 353 | 1.25e-145 | 415 |
| MS.gene65617.t1 | MTR_1g028360 | 64.379 | 306 | 96 | 3 | 44 | 337 | 53 | 357 | 5.72e-139 | 399 |
| MS.gene65617.t1 | MTR_3g078190 | 57.483 | 294 | 104 | 2 | 51 | 340 | 41 | 317 | 1.29e-128 | 371 |
| MS.gene65617.t1 | MTR_5g006140 | 35.965 | 228 | 137 | 7 | 71 | 293 | 42 | 265 | 3.89e-38 | 139 |
| MS.gene65617.t1 | MTR_5g006140 | 36.321 | 212 | 128 | 5 | 71 | 277 | 42 | 251 | 7.07e-38 | 136 |
| MS.gene65617.t1 | MTR_1g015300 | 31.136 | 273 | 158 | 9 | 45 | 295 | 11 | 275 | 9.70e-37 | 135 |
| MS.gene65617.t1 | MTR_3g104960 | 29.412 | 255 | 152 | 11 | 45 | 290 | 28 | 263 | 6.58e-28 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65617.t1 | AT1G23820 | 79.456 | 331 | 63 | 4 | 12 | 342 | 9 | 334 | 0.0 | 544 |
| MS.gene65617.t1 | AT1G70310 | 76.385 | 343 | 77 | 2 | 1 | 342 | 1 | 340 | 0.0 | 541 |
| MS.gene65617.t1 | AT1G23820 | 80.899 | 267 | 47 | 3 | 12 | 278 | 53 | 315 | 3.39e-159 | 449 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 49 | 355 | 9.86e-145 | 413 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 49 | 355 | 9.86e-145 | 413 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 49 | 355 | 9.86e-145 | 413 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 49 | 355 | 9.86e-145 | 413 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 49 | 355 | 9.86e-145 | 413 |
| MS.gene65617.t1 | AT5G53120 | 65.909 | 308 | 88 | 3 | 48 | 339 | 66 | 372 | 2.28e-144 | 413 |
| MS.gene65617.t1 | AT5G53120 | 60.299 | 335 | 89 | 4 | 48 | 339 | 49 | 382 | 7.01e-140 | 402 |
| MS.gene65617.t1 | AT5G19530 | 32.171 | 258 | 163 | 7 | 68 | 320 | 47 | 297 | 3.64e-39 | 142 |
| MS.gene65617.t1 | AT5G19530 | 32.171 | 258 | 163 | 7 | 68 | 320 | 40 | 290 | 3.73e-39 | 141 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGGAAGACCATGACTTTC+TGG | 0.202805 | 8.2:-37951033 | None:intergenic |
| ATCATCTCTTTGTCTCTTCA+TGG | 0.223026 | 8.2:-37947570 | None:intergenic |
| ATATGGCAAGGTTCTTGTTT+TGG | 0.280455 | 8.2:+37951164 | MS.gene65617:CDS |
| CTCTATTTCTAACCCCAAAA+AGG | 0.307016 | 8.2:+37951257 | MS.gene65617:CDS |
| GGTGTTTCATCTGTGATTCC+TGG | 0.309804 | 8.2:+37947668 | MS.gene65617:CDS |
| GATGATGAAGAAGAGAATAA+TGG | 0.312917 | 8.2:+37947587 | MS.gene65617:CDS |
| CCAGAAAGTCATGGTCTTCC+AGG | 0.315838 | 8.2:+37951033 | MS.gene65617:CDS |
| ATGATGAAGAAGAGAATAAT+GGG | 0.324828 | 8.2:+37947588 | MS.gene65617:CDS |
| TTGGTTATTGGTGGAGGTGA+TGG | 0.328542 | 8.2:+37951647 | MS.gene65617:CDS |
| TTTGAAAATCTGACGACAAT+TGG | 0.351640 | 8.2:-37952783 | None:intergenic |
| ACTGCATCATAAGTTCCTTC+TGG | 0.359338 | 8.2:-37952510 | None:intergenic |
| GTAGATGAGTGATCATTTCT+TGG | 0.372101 | 8.2:-37951227 | None:intergenic |
| GATGAAACACCATTACCATT+TGG | 0.374994 | 8.2:-37947656 | None:intergenic |
| CATTTGTGAAATTGACAATA+TGG | 0.376368 | 8.2:+37951718 | MS.gene65617:CDS |
| CATGGCTGCTGCAGAGAGTA+TGG | 0.377150 | 8.2:+37947532 | None:intergenic |
| TTAAACCCTACTGCAACTTC+AGG | 0.398829 | 8.2:-37952151 | None:intergenic |
| GCACTCAGGCTGAAAGTATA+TGG | 0.405219 | 8.2:+37952729 | MS.gene65617:CDS |
| ATGAAACACCATTACCATTT+GGG | 0.414247 | 8.2:-37947655 | None:intergenic |
| AACTTATGATGCAGTTATAG+TGG | 0.422946 | 8.2:+37952518 | MS.gene65617:CDS |
| GTTATTGGTGGAGGTGATGG+TGG | 0.427899 | 8.2:+37951650 | MS.gene65617:CDS |
| GTGGATTCATCTGATCCTAT+TGG | 0.434928 | 8.2:+37952537 | MS.gene65617:CDS |
| AAAACTAACCTGGCCACATT+GGG | 0.439033 | 8.2:-37947709 | None:intergenic |
| TGCAGAGAGTATGGTGGAAC+TGG | 0.481978 | 8.2:+37947541 | MS.gene65617:CDS |
| TGAAAGTATATGGCTTCATA+TGG | 0.483697 | 8.2:+37952739 | MS.gene65617:CDS |
| AAGGCTCTGTCAATTATGCT+TGG | 0.485127 | 8.2:+37952804 | MS.gene65617:CDS |
| AAAACATACCTTGGATATGT+AGG | 0.492840 | 8.2:-37952836 | None:intergenic |
| GAAATTAGCCCAATGTGGCC+AGG | 0.496157 | 8.2:+37947701 | MS.gene65617:CDS |
| GTGTTTCATCTGTGATTCCT+GGG | 0.503605 | 8.2:+37947669 | MS.gene65617:CDS |
| TCGTTTGCGAGGAGGAAGAT+TGG | 0.509808 | 8.2:+37954062 | MS.gene65617:CDS |
| GTGGCAAGGGCTCTTCGTCC+AGG | 0.512123 | 8.2:+37952695 | MS.gene65617:CDS |
| TGTTGAAGGTGGTGGTGATA+AGG | 0.524499 | 8.2:+37947631 | MS.gene65617:CDS |
| TGAGTGCACATAACTCCTCC+TGG | 0.525919 | 8.2:-37952713 | None:intergenic |
| CAAACATCCAGTGAATCCTA+TGG | 0.529311 | 8.2:+37953851 | MS.gene65617:CDS |
| TAAACCCTACTGCAACTTCA+GGG | 0.530870 | 8.2:-37952150 | None:intergenic |
| GCTTCATATGGATATAATTG+AGG | 0.531349 | 8.2:+37952751 | MS.gene65617:CDS |
| GTTTCAACAATGGATGTTGA+AGG | 0.531892 | 8.2:+37947617 | MS.gene65617:CDS |
| TTTATGCTTTGCTCAACTGA+GGG | 0.534590 | 8.2:+37953813 | MS.gene65617:CDS |
| ATGAGTGATCATTTCTTGGT+AGG | 0.535102 | 8.2:-37951223 | None:intergenic |
| TGGGGTTTCTGTTTCAACAA+TGG | 0.540223 | 8.2:+37947607 | MS.gene65617:CDS |
| ATATCCTTAAGGTCCTGCTC+AGG | 0.540360 | 8.2:+37952643 | MS.gene65617:intron |
| GAGTTATTCAGCTCACAGAA+AGG | 0.548553 | 8.2:+37951190 | MS.gene65617:CDS |
| ATCTCTTTGTCTCTTCATGG+AGG | 0.564464 | 8.2:-37947567 | None:intergenic |
| AGTTATTCAGCTCACAGAAA+GGG | 0.568813 | 8.2:+37951191 | MS.gene65617:CDS |
| GCAGTCATCAACATATGGCA+AGG | 0.573215 | 8.2:+37951152 | MS.gene65617:intron |
| GGTGGTGATAAGGACCCAAA+TGG | 0.579962 | 8.2:+37947641 | MS.gene65617:CDS |
| TCTGTTTGCCGTCGTTTGCG+AGG | 0.581679 | 8.2:+37954051 | MS.gene65617:CDS |
| GGGTTAGAAATAGAGCAAAG+AGG | 0.598026 | 8.2:-37951249 | None:intergenic |
| GATAAGGACCCAAATGGTAA+TGG | 0.604632 | 8.2:+37947647 | MS.gene65617:CDS |
| TCAGAGTTGTAAAATTTGAG+TGG | 0.611670 | 8.2:-37953903 | None:intergenic |
| CCACGTGTGACTCTTCGCAT+TGG | 0.612172 | 8.2:+37952178 | MS.gene65617:CDS |
| GTGACTCTTCGCATTGGTGA+TGG | 0.620618 | 8.2:+37952184 | MS.gene65617:CDS |
| AGGAGGAGTTATGTGCACTC+AGG | 0.621343 | 8.2:+37952715 | MS.gene65617:CDS |
| GTTTGCCGTCGTTTGCGAGG+AGG | 0.626127 | 8.2:+37954054 | MS.gene65617:CDS |
| CTACAGTTCCTACATATCCA+AGG | 0.636790 | 8.2:+37952828 | MS.gene65617:CDS |
| GTCAGATTACCAGAAAGTCA+TGG | 0.643976 | 8.2:+37951024 | MS.gene65617:CDS |
| ATCTTCCTCCTCGCAAACGA+CGG | 0.645194 | 8.2:-37954059 | None:intergenic |
| AATGACGTGCTACTTCTCGC+AGG | 0.646893 | 8.2:-37951676 | None:intergenic |
| TTTCATCTGTGATTCCTGGG+TGG | 0.656590 | 8.2:+37947672 | MS.gene65617:CDS |
| ACAATGGATGTTGAAGGTGG+TGG | 0.657096 | 8.2:+37947623 | MS.gene65617:CDS |
| GCAAGGGCTCTTCGTCCAGG+AGG | 0.657812 | 8.2:+37952698 | MS.gene65617:CDS |
| TGATGAAGAAGAGAATAATG+GGG | 0.657977 | 8.2:+37947589 | MS.gene65617:CDS |
| GGATGTTTGAAATCAACAAG+AGG | 0.663795 | 8.2:-37953837 | None:intergenic |
| TTTCTGAAATTAGCCCAATG+TGG | 0.672641 | 8.2:+37947696 | MS.gene65617:CDS |
| CTAATTTCAGAAAACCACCC+AGG | 0.684797 | 8.2:-37947686 | None:intergenic |
| TCAACAATGGATGTTGAAGG+TGG | 0.691448 | 8.2:+37947620 | MS.gene65617:CDS |
| GGCTGCTGCAGAGAGTATGG+TGG | 0.698354 | 8.2:+37947535 | MS.gene65617:CDS |
| CCAATGCGAAGAGTCACACG+TGG | 0.702718 | 8.2:-37952178 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 37947533 | 37954100 | 37947533 | ID=MS.gene65617 |
| chr8.2 | mRNA | 37947533 | 37954100 | 37947533 | ID=MS.gene65617.t1;Parent=MS.gene65617 |
| chr8.2 | exon | 37947533 | 37947722 | 37947533 | ID=MS.gene65617.t1.exon1;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37947533 | 37947722 | 37947533 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37950978 | 37951054 | 37950978 | ID=MS.gene65617.t1.exon2;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37950978 | 37951054 | 37950978 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37951156 | 37951278 | 37951156 | ID=MS.gene65617.t1.exon3;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37951156 | 37951278 | 37951156 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37951644 | 37951748 | 37951644 | ID=MS.gene65617.t1.exon4;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37951644 | 37951748 | 37951644 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37952133 | 37952205 | 37952133 | ID=MS.gene65617.t1.exon5;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37952133 | 37952205 | 37952133 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37952487 | 37952558 | 37952487 | ID=MS.gene65617.t1.exon6;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37952487 | 37952558 | 37952487 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37952654 | 37952849 | 37952654 | ID=MS.gene65617.t1.exon7;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37952654 | 37952849 | 37952654 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37953800 | 37953926 | 37953800 | ID=MS.gene65617.t1.exon8;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37953800 | 37953926 | 37953800 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| chr8.2 | exon | 37954035 | 37954100 | 37954035 | ID=MS.gene65617.t1.exon9;Parent=MS.gene65617.t1 |
| chr8.2 | CDS | 37954035 | 37954100 | 37954035 | ID=cds.MS.gene65617.t1;Parent=MS.gene65617.t1 |
| Gene Sequence |
| Protein sequence |
