AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66785

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66785.t1 MTR_4g053675 98.259 1034 15 1 1 1034 1 1031 0.0 2103
MS.gene66785.t1 MTR_2g086140 80.263 988 176 8 1 982 1 975 0.0 1620
MS.gene66785.t1 MTR_8g093540 27.206 408 211 10 11 376 11 374 1.13e-35 144
MS.gene66785.t1 MTR_3g106080 28.205 195 108 2 16 210 464 626 1.78e-18 91.3
MS.gene66785.t1 MTR_4g095058 24.789 355 197 9 16 370 292 576 1.12e-16 85.1
MS.gene66785.t1 MTR_4g095058 24.789 355 197 9 16 370 290 574 1.19e-16 85.1
MS.gene66785.t1 MTR_5g016350 23.662 355 201 10 16 370 297 581 1.80e-15 81.3
MS.gene66785.t1 MTR_5g016350 23.662 355 201 10 16 370 233 517 2.12e-15 80.9
MS.gene66785.t1 MTR_8g038550 26.984 189 104 4 12 198 308 464 2.16e-13 75.1
MS.gene66785.t1 MTR_1g072140 31.481 162 93 3 65 226 22 165 4.48e-13 72.0
MS.gene66785.t1 MTR_1g072140 29.861 144 84 1 62 205 61 187 5.97e-12 68.6
MS.gene66785.t1 MTR_5g016370 23.380 355 202 10 16 370 309 593 7.42e-13 73.2
MS.gene66785.t1 MTR_5g016370 25.322 233 121 5 5 202 340 554 9.75e-12 69.3
MS.gene66785.t1 MTR_2g034500 30.216 139 77 2 63 198 234 355 1.98e-12 72.4
MS.gene66785.t1 MTR_7g104550 27.313 227 119 8 15 220 18 219 3.13e-12 69.7
MS.gene66785.t1 MTR_4g105700 26.768 198 122 4 28 224 420 595 5.81e-12 70.5
MS.gene66785.t1 MTR_1g087120 33.094 139 73 3 63 198 319 440 5.98e-12 70.9
MS.gene66785.t1 MTR_1g054300 22.680 388 225 12 12 379 118 450 1.24e-11 68.9
MS.gene66785.t1 MTR_4g119620 27.228 202 116 4 16 217 30 200 1.68e-11 67.4
MS.gene66785.t1 MTR_6g004040 24.865 185 107 3 28 212 32 184 2.79e-11 68.2
MS.gene66785.t1 MTR_5g035980 31.138 167 106 4 16 178 246 407 5.12e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66785.t1 AT3G44530 72.661 962 245 9 1 960 1 946 0.0 1398
MS.gene66785.t1 AT3G44530 69.880 996 248 11 1 960 1 980 0.0 1385
MS.gene66785.t1 AT3G44530 70.940 819 224 8 144 960 2 808 0.0 1152
MS.gene66785.t1 AT3G44530 67.638 686 208 8 277 960 1 674 0.0 890
MS.gene66785.t1 AT5G64630 28.395 405 220 11 11 380 11 380 1.11e-39 154
MS.gene66785.t1 AT5G64630 27.901 405 222 10 11 380 11 380 2.36e-39 151
MS.gene66785.t1 AT5G64630 28.779 344 180 10 70 380 10 321 5.80e-32 130
MS.gene66785.t1 AT5G67320 22.642 371 185 10 16 370 327 611 2.15e-17 87.8
MS.gene66785.t1 AT5G67320 27.679 224 109 5 10 198 363 568 1.21e-12 72.4
MS.gene66785.t1 AT5G25150 28.962 183 98 2 16 198 463 613 5.03e-16 83.6
MS.gene66785.t1 AT5G25150 23.111 225 137 3 11 201 352 574 2.94e-11 68.2
MS.gene66785.t1 AT5G25150 28.962 183 98 2 16 198 463 613 5.40e-16 83.2
MS.gene66785.t1 AT5G25150 23.111 225 137 3 11 201 352 574 3.13e-11 68.2
MS.gene66785.t1 AT5G13480 27.513 189 103 4 12 198 259 415 4.33e-13 73.9
MS.gene66785.t1 AT5G13480 27.513 189 103 4 12 198 236 392 4.52e-13 73.9
MS.gene66785.t1 AT5G13480 27.513 189 103 4 12 198 236 392 4.81e-13 73.9
MS.gene66785.t1 AT5G13480 27.513 189 103 4 12 198 248 404 5.57e-13 73.6
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 47 175 8.78e-13 73.6
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 47 175 8.78e-13 73.6
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 47 175 9.70e-13 73.2
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 228 356 1.05e-12 73.2
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 229 357 1.06e-12 73.2
MS.gene66785.t1 AT2G47410 30.822 146 79 3 63 203 229 357 1.14e-12 73.2
MS.gene66785.t1 AT4G02730 28.144 167 101 3 61 226 34 182 2.70e-12 69.7
MS.gene66785.t1 AT5G08390 26.596 188 106 3 28 215 32 187 2.81e-12 71.6
MS.gene66785.t1 AT3G49660 26.457 223 120 6 18 234 29 213 1.13e-11 67.8
MS.gene66785.t1 AT5G49430 30.201 149 84 3 63 208 238 369 1.80e-11 69.3
MS.gene66785.t1 AT5G49430 30.201 149 84 3 63 208 238 369 1.80e-11 69.3

Find 283 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGGATCACTTTATTTA+TGG 0.129245 4.3:-46644430 MS.gene66785:CDS
CTTTGCAGATATAACTTTAA+TGG 0.177954 4.3:+46642815 None:intergenic
GTTCAGCAGGAAAATATTTC+TGG 0.195639 4.3:-46645484 MS.gene66785:CDS
GCAACCCTACGGGATCATTT+TGG 0.196194 4.3:-46647768 MS.gene66785:CDS
AGCTCAGTTGGCCTCTTCTT+TGG 0.207933 4.3:-46641904 MS.gene66785:CDS
TTGAACTTGACCACAATAAT+TGG 0.212571 4.3:+46646557 None:intergenic
GGACCATCACTCTTCTTATT+AGG 0.233543 4.3:+46645631 None:intergenic
GTTGGCCTCTTCTTTGGCTT+TGG 0.239980 4.3:-46641898 MS.gene66785:CDS
GGGCAGAATGCCTTGGCTTC+TGG 0.250053 4.3:+46646636 None:intergenic
TGGTTCTCCTCTTGTTGTTT+TGG 0.252310 4.3:-46642781 MS.gene66785:CDS
GAGAAGGTTCCGGCCTCTTC+TGG 0.260380 4.3:-46645271 MS.gene66785:CDS
ACAGGAACTCCAACTGCTTC+TGG 0.265909 4.3:+46645505 None:intergenic
TGGTGAACTGGCTGCTTTGC+AGG 0.267347 4.3:-46642643 MS.gene66785:CDS
GCATTTGCACAACTGTTCTA+AGG 0.270517 4.3:-46647031 MS.gene66785:CDS
TTTGCCAGCTCTTGGAGTTT+GGG 0.272667 4.3:-46642678 MS.gene66785:CDS
TTGGCCTCTTCTTTGGCTTT+GGG 0.274910 4.3:-46641897 MS.gene66785:CDS
CACATTGGCCAGTGGTAGTT+TGG 0.276268 4.3:-46647087 MS.gene66785:CDS
AGTATCAGCAATTTCGTATT+CGG 0.290589 4.3:+46639788 None:intergenic
AAAAGGATCACTTTATTTAT+GGG 0.295941 4.3:-46644429 MS.gene66785:CDS
ATGATAAAACAGTCATTATC+TGG 0.307219 4.3:-46646941 MS.gene66785:CDS
GGCATTGGCGATATTCATTA+GGG 0.318800 4.3:+46641871 None:intergenic
TAAAGTAGGTGGATCTTAAT+TGG 0.320161 4.3:-46647124 MS.gene66785:intron
TTCATCACAGTCTACAAATA+TGG 0.321824 4.3:+46644478 None:intergenic
CCTAGTCCAACCTGGCTTGC+GGG 0.328585 4.3:+46642599 None:intergenic
AGGCATTGGCGATATTCATT+AGG 0.328755 4.3:+46641870 None:intergenic
CATTACTCATATTCCATATA+TGG 0.333463 4.3:+46647054 None:intergenic
TCCCAACTGCTCAACATTGA+TGG 0.338707 4.3:+46645237 None:intergenic
CTTTGGTCTGATAGGATATC+AGG 0.345362 4.3:-46645025 MS.gene66785:CDS
CCATACAATGTTATTGCAAT+TGG 0.352122 4.3:-46646431 MS.gene66785:CDS
CATGGAAGGTATATTGCATC+TGG 0.358585 4.3:-46647717 MS.gene66785:CDS
TCATTTACGTATGTTCCTAA+TGG 0.361413 4.3:+46639696 None:intergenic
ATGTTCAACCTGGTGGCCTT+AGG 0.362491 4.3:-46649019 MS.gene66785:CDS
TGCCTGCCGGATGGTCCAAT+GGG 0.364491 4.3:-46646484 MS.gene66785:CDS
CTGCCTGCCGGATGGTCCAA+TGG 0.367361 4.3:-46646485 MS.gene66785:CDS
TGATTGCTGAGAAACCTATC+TGG 0.367498 4.3:-46649076 MS.gene66785:CDS
TGCATCTGGGTCGGACGATC+AGG 0.372181 4.3:-46647703 MS.gene66785:CDS
GGCCTTGCTTCTAAACAAAT+AGG 0.373501 4.3:+46645139 None:intergenic
CAACTCCATTGTTACCCCTT+TGG 0.374334 4.3:+46645413 None:intergenic
AACATCATCGGATAAGAGTT+TGG 0.386540 4.3:-46641367 MS.gene66785:CDS
TGGCTCTTTATTTCCAGTCT+TGG 0.390889 4.3:+46646457 None:intergenic
TTCCAGCCATGGCATCAAAT+AGG 0.391006 4.3:-46639851 MS.gene66785:CDS
GAGACTGCTCAACGAATTCA+TGG 0.391577 4.3:-46639820 MS.gene66785:CDS
CTAATAGAGAGTGGTCCATT+AGG 0.392622 4.3:-46639711 MS.gene66785:CDS
TGGACAACACCATCCATATA+TGG 0.393575 4.3:-46647067 MS.gene66785:CDS
TGCACATGATCAAATTGTTC+AGG 0.396758 4.3:-46639583 MS.gene66785:CDS
TCCAATGGGGCTTCCAAGAC+TGG 0.399767 4.3:-46646470 MS.gene66785:CDS
GAAATCGTACATAGGACAAT+AGG 0.402440 4.3:+46641850 None:intergenic
GGGCCACTCTAGCTTGGTTA+AGG 0.402923 4.3:-46647009 MS.gene66785:CDS
CCTTCATGCCTAACCCAGAT+AGG 0.405755 4.3:+46649062 None:intergenic
CCAATTGCAATAACATTGTA+TGG 0.407012 4.3:+46646431 None:intergenic
CTCATGCCGCTCTTTCGATT+AGG 0.411524 4.3:-46645195 MS.gene66785:CDS
GCTTGGTTAAGGGCGTTGCT+TGG 0.411526 4.3:-46646998 MS.gene66785:CDS
TGAATTCGTTGAGCAGTCTC+TGG 0.412571 4.3:+46639823 None:intergenic
CATGAGAGGAAGCCTGGTTC+AGG 0.413791 4.3:-46647669 MS.gene66785:CDS
CTTGGTTAAGGGCGTTGCTT+GGG 0.415567 4.3:-46646997 MS.gene66785:CDS
GAGTCTCAAGTGTTGGTTAA+GGG 0.415688 4.3:-46642730 MS.gene66785:CDS
CCTTGTGATTGAGAAGGTTC+CGG 0.415801 4.3:-46645281 MS.gene66785:CDS
TTGGCAGTTGGTTGTGAGGA+TGG 0.416645 4.3:-46644968 MS.gene66785:CDS
CTCTATTCAAAGTGGTGAAC+TGG 0.417934 4.3:-46642655 MS.gene66785:CDS
TGTTATTGCAATTGGAAGTC+AGG 0.418050 4.3:-46646423 MS.gene66785:CDS
CAAGAACCTCATTCCTGATC+TGG 0.419160 4.3:+46639555 None:intergenic
AGTTTCCTTGGACCTCCAAC+TGG 0.422515 4.3:-46641402 MS.gene66785:CDS
GGCGTTGCTTGGGATCCTAT+TGG 0.424316 4.3:-46646987 MS.gene66785:CDS
TTTAAATCTGAATTTCTGCC+AGG 0.427338 4.3:+46645346 None:intergenic
TTCCTATTTGATGCCATGGC+TGG 0.431604 4.3:+46639849 None:intergenic
GACTTTCCTATTTGATGCCA+TGG 0.433487 4.3:+46639845 None:intergenic
GAGAAACCAACGAGTCTTGA+AGG 0.433506 4.3:+46644384 None:intergenic
CTCAAACACTTTGGTCTGAT+AGG 0.435314 4.3:-46645033 MS.gene66785:CDS
CTTCTTGCTTGGTGTTTGCT+TGG 0.435321 4.3:+46645756 None:intergenic
CATGCTGTTAATGACATTGC+AGG 0.440016 4.3:-46645109 MS.gene66785:CDS
TTGGGTCTGTCAACTGTGTA+AGG 0.442920 4.3:-46647749 MS.gene66785:CDS
AATAGTCTTACAAGCACAAC+AGG 0.446613 4.3:+46641336 None:intergenic
CATTCCCCTCTCTCGAGGAC+AGG 0.447521 4.3:+46646614 None:intergenic
CTATATTCTCTTTGCTTCAC+AGG 0.448882 4.3:+46645556 None:intergenic
CTTAGGTTTGCTACTGGTGG+TGG 0.448989 4.3:-46649002 MS.gene66785:CDS
GACAGGGAAGCCAAGTTCTT+CGG 0.449576 4.3:+46645450 None:intergenic
AGCTCATCTGCTAAAGATAC+AGG 0.452038 4.3:-46644344 MS.gene66785:intron
AAATATTTGGCCCGCAAGCC+AGG 0.452423 4.3:-46642609 MS.gene66785:CDS
TCCAGTCTTGGAAGCCCCAT+TGG 0.453698 4.3:+46646469 None:intergenic
CTGAGAAACCTATCTGGGTT+AGG 0.453815 4.3:-46649070 MS.gene66785:CDS
TTGGGAAATTCGACGACTAC+TGG 0.454060 4.3:-46645220 MS.gene66785:CDS
TTAGCAACCACCTTCTTGCT+TGG 0.458208 4.3:+46645745 None:intergenic
GGTTCAGGTACCACAGAATT+CGG 0.460753 4.3:-46647654 MS.gene66785:CDS
AGCCACCGGATATTGAAAAC+TGG 0.461185 4.3:-46647623 MS.gene66785:CDS
TTTAATGCATCACCACCAAC+GGG 0.461912 4.3:+46645610 None:intergenic
GGCCACTCTAGCTTGGTTAA+GGG 0.463838 4.3:-46647008 MS.gene66785:CDS
CTATCAGACCAAAGTGTTTG+AGG 0.465507 4.3:+46645034 None:intergenic
AGGATCGAACTATAACTGTT+TGG 0.466858 4.3:-46646403 MS.gene66785:CDS
CTGTTGCTGGTGACTAGAAA+AGG 0.466988 4.3:-46644446 MS.gene66785:CDS
CCGGCCTCTTCTGGCAGAGA+TGG 0.467613 4.3:-46645262 MS.gene66785:CDS
GAAGTCTGTGAGAGTTTCCT+TGG 0.469315 4.3:-46641414 MS.gene66785:CDS
AACCGATCCATCCAAGGAAC+AGG 0.470436 4.3:+46645930 None:intergenic
GGATAGTTGACAAGAAAGGT+GGG 0.470622 4.3:-46645174 MS.gene66785:CDS
CTCACAGGACAGATGGTCAC+TGG 0.471964 4.3:-46646896 MS.gene66785:CDS
TGTTCAGGATACACCAGATC+AGG 0.473006 4.3:-46639568 MS.gene66785:CDS
TTGTAGACTGTGATGAAAGT+TGG 0.474659 4.3:-46644472 MS.gene66785:CDS
TTGAATCTTTGCAGGGAATG+AGG 0.475545 4.3:-46639902 MS.gene66785:intron
TCTAAGGGGCCACTCTAGCT+TGG 0.477426 4.3:-46647015 MS.gene66785:CDS
TACCTATTTGTTTAGAAGCA+AGG 0.477895 4.3:-46645141 MS.gene66785:CDS
TCTGCAAAGCTATCAAAGTC+TGG 0.478840 4.3:-46642801 MS.gene66785:CDS
GGAAGCCCCATTGGACCATC+CGG 0.479663 4.3:+46646478 None:intergenic
ACCTAAGGTACTTGTTCTTA+CGG 0.479901 4.3:+46645375 None:intergenic
TCAGATTTAAAAGAACGCTC+AGG 0.480310 4.3:-46645334 MS.gene66785:CDS
ATAATTCTCCTCCTTCCATC+AGG 0.480332 4.3:+46645529 None:intergenic
AACCATCAATGTTGAGCAGT+TGG 0.482044 4.3:-46645239 MS.gene66785:CDS
TGCTCACTTGGAAGCTCAGT+TGG 0.482764 4.3:-46641916 MS.gene66785:CDS
GCAGTTCAATCCGAAGAACT+TGG 0.483559 4.3:-46645460 MS.gene66785:CDS
TTATCTGGAAAACTAGTGAT+TGG 0.485573 4.3:-46646926 MS.gene66785:CDS
GCCCCATTGGACCATCCGGC+AGG 0.486063 4.3:+46646482 None:intergenic
ACCATCAATGTTGAGCAGTT+GGG 0.486119 4.3:-46645238 MS.gene66785:CDS
AGTGCATACACGTGCTCACT+TGG 0.486258 4.3:-46641928 MS.gene66785:CDS
CCTTGGACCTCCAACTGGCA+TGG 0.489223 4.3:-46641397 MS.gene66785:CDS
AATTTCTGTTTCCCTCATCA+TGG 0.490984 4.3:+46645072 None:intergenic
AGTTGGAGTTCCTGTTCAGC+AGG 0.491571 4.3:-46645497 MS.gene66785:CDS
CGTGTCTTCTGAGCACCAAA+GGG 0.491642 4.3:-46645428 MS.gene66785:CDS
TCTTCAGCCATGCCAGTTGG+AGG 0.491804 4.3:+46641390 None:intergenic
CTGGCCAATGTGGAATCATC+AGG 0.494501 4.3:+46647098 None:intergenic
AGCAATGCCTACCATGATGA+TGG 0.496254 4.3:-46644510 MS.gene66785:CDS
TCTGCCCCTGTCCTCGAGAG+AGG 0.497072 4.3:-46646620 MS.gene66785:CDS
CTTGCTATAAAAGATCCAAT+AGG 0.497902 4.3:+46646972 None:intergenic
TGAAAGTTGGAAGCTGTTGC+TGG 0.500343 4.3:-46644459 MS.gene66785:CDS
GAGCTGGCAAAATTTGATGC+AGG 0.500810 4.3:+46642690 None:intergenic
TCAACCTTCTTCAGACGACT+TGG 0.501073 4.3:-46646701 MS.gene66785:CDS
TCCATTGATGTTCAACCTGG+TGG 0.501384 4.3:-46649026 MS.gene66785:CDS
CCCGCAAGCCAGGTTGGACT+AGG 0.501584 4.3:-46642599 MS.gene66785:intron
TGCCTGTTCCTTGGATGGAT+CGG 0.501589 4.3:-46645932 MS.gene66785:CDS
AACGCTCAGGGGTTACCGCT+AGG 0.502120 4.3:-46645321 MS.gene66785:CDS
GTCTGTCAACTGTGTAAGGT+GGG 0.504299 4.3:-46647745 MS.gene66785:CDS
TGAGTCTCAAGTGTTGGTTA+AGG 0.504728 4.3:-46642731 MS.gene66785:CDS
AGCAAACTTAGCAGAGAGTC+CGG 0.504934 4.3:-46645806 MS.gene66785:CDS
CAGATTTAAAAGAACGCTCA+GGG 0.505055 4.3:-46645333 MS.gene66785:CDS
GCCACCAGGTTGAACATCAA+TGG 0.505751 4.3:+46649025 None:intergenic
CCTATCTGGGTTAGGCATGA+AGG 0.506355 4.3:-46649062 MS.gene66785:CDS
TTAATTCATGAGAGGAAGCC+TGG 0.506476 4.3:-46647675 MS.gene66785:CDS
CCTATGTACGATTTCTTGCA+AGG 0.508448 4.3:-46641842 MS.gene66785:intron
GGTGGCCTTAGGTTTGCTAC+TGG 0.511390 4.3:-46649008 MS.gene66785:CDS
ATTAGGATAGTTGACAAGAA+AGG 0.513380 4.3:-46645178 MS.gene66785:CDS
GGCCTTAGGTTTGCTACTGG+TGG 0.513426 4.3:-46649005 MS.gene66785:CDS
GTTTCTTCAGCCATGCCAGT+TGG 0.515748 4.3:+46641387 None:intergenic
AGGATAGTTGACAAGAAAGG+TGG 0.515769 4.3:-46645175 MS.gene66785:CDS
CTGGAAGAATGTCTTCGATA+AGG 0.516463 4.3:+46639868 None:intergenic
AGGACAGGGGCAGAATGCCT+TGG 0.518488 4.3:+46646629 None:intergenic
CATTTGCACAACTGTTCTAA+GGG 0.519738 4.3:-46647030 MS.gene66785:CDS
AGAATTATTCCAGAAGCAGT+TGG 0.520758 4.3:-46645514 MS.gene66785:CDS
AGAGGCTTCTTGCAACCCTA+CGG 0.521890 4.3:-46647779 MS.gene66785:CDS
AAAGTGTTGTGGATTTATCC+TGG 0.522076 4.3:-46646331 MS.gene66785:intron
GAGAAGATTTACACAAAGTG+TGG 0.523098 4.3:-46644539 MS.gene66785:intron
TTGACATGAGTCTCAAGTGT+TGG 0.523601 4.3:-46642737 MS.gene66785:CDS
TATATTCTCTTTGCTTCACA+GGG 0.524792 4.3:+46645557 None:intergenic
CTGTCTCCTTCAAGACTCGT+TGG 0.528643 4.3:-46644390 MS.gene66785:CDS
TCTTGAAACAGGAGCCCTGA+TGG 0.531488 4.3:-46645967 MS.gene66785:intron
TCGTGTCTTCTGAGCACCAA+AGG 0.532080 4.3:-46645429 MS.gene66785:CDS
ATGCTGTATATTGAATCCTC+AGG 0.532196 4.3:+46639669 None:intergenic
CATATATGGAATATGAGTAA+TGG 0.534441 4.3:-46647053 MS.gene66785:CDS
ACCGTAAGAACAAGTACCTT+AGG 0.536470 4.3:-46645376 MS.gene66785:CDS
ACCTAGTCCAACCTGGCTTG+CGG 0.538398 4.3:+46642598 None:intergenic
ATTCCCCTCTCTCGAGGACA+GGG 0.540099 4.3:+46646615 None:intergenic
TGGGAAATGCATCCATGATG+AGG 0.540434 4.3:-46645084 MS.gene66785:CDS
GTTTAATGCATCACCACCAA+CGG 0.540968 4.3:+46645609 None:intergenic
CTGCCCCTGTCCTCGAGAGA+GGG 0.541522 4.3:-46646619 MS.gene66785:CDS
TTCTCCATTGATGTTCAACC+TGG 0.541547 4.3:-46649029 MS.gene66785:CDS
CGCCCTTAACCAAGCTAGAG+TGG 0.542102 4.3:+46647006 None:intergenic
CCATCCAAGTCGTCTGAAGA+AGG 0.543050 4.3:+46646697 None:intergenic
TGGTATAGAATGACTGATGA+CGG 0.543456 4.3:-46641951 MS.gene66785:intron
CGAAGACATTCTTCCAGCCA+TGG 0.543845 4.3:-46639862 MS.gene66785:CDS
CCTTCTTCAGACGACTTGGA+TGG 0.544713 4.3:-46646697 MS.gene66785:CDS
CAGAAATTGCTTGCACTAGA+GGG 0.545773 4.3:-46645057 MS.gene66785:CDS
TCTGCCAGGTGCTCCACCTA+AGG 0.546653 4.3:+46645360 None:intergenic
AATTCTGTGGTACCTGAACC+AGG 0.546984 4.3:+46647657 None:intergenic
ATTTGGCCCGCAAGCCAGGT+TGG 0.549243 4.3:-46642605 MS.gene66785:CDS
GAGGCTTCTTGCAACCCTAC+GGG 0.550771 4.3:-46647778 MS.gene66785:CDS
CAACGAGTCTTGAAGGAGAC+AGG 0.551079 4.3:+46644391 None:intergenic
ACAGAAATTGCTTGCACTAG+AGG 0.552254 4.3:-46645058 MS.gene66785:CDS
GAAATGAAGTCTCTGCCTGC+CGG 0.554698 4.3:-46646497 MS.gene66785:CDS
CTGTCCTCGAGAGAGGGGAA+TGG 0.555734 4.3:-46646613 MS.gene66785:CDS
TCATTAACAGCATGTTCTCT+CGG 0.557271 4.3:+46645118 None:intergenic
AGAGCCCAAACTCCAAGAGC+TGG 0.558972 4.3:+46642674 None:intergenic
CCATCTCTGCCAGAAGAGGC+CGG 0.559147 4.3:+46645262 None:intergenic
GGTGTTGTCCAAACTACCAC+TGG 0.560714 4.3:+46647079 None:intergenic
TGAGAGCCTTGTGATTGAGA+AGG 0.561272 4.3:-46645287 MS.gene66785:CDS
ATTCGGTAGTGGAGAGCCAC+CGG 0.561465 4.3:-46647637 MS.gene66785:CDS
ACGCTCAGGGGTTACCGCTA+GGG 0.565882 4.3:-46645320 MS.gene66785:CDS
GAAGATGTCTTGAGTAACAC+TGG 0.566233 4.3:+46639747 None:intergenic
AGAGAATATAGACGTCCTGA+TGG 0.566622 4.3:-46645544 MS.gene66785:CDS
GCTTCTAGCAATAACTGTGC+CGG 0.567315 4.3:+46645787 None:intergenic
TTGTGAGGATGGATGCCTGC+AGG 0.567992 4.3:-46644957 MS.gene66785:intron
GGTCTGTCAACTGTGTAAGG+TGG 0.568847 4.3:-46647746 MS.gene66785:CDS
CGAGTTGCCAAAACAACAAG+AGG 0.570729 4.3:+46642774 None:intergenic
CTCATGGTTTCCAGAAGCCA+AGG 0.573823 4.3:-46646646 MS.gene66785:CDS
CTAGAGGGCCTCAAACACTT+TGG 0.574402 4.3:-46645042 MS.gene66785:CDS
GTGATTGGAGTCTTGCTCAC+AGG 0.575006 4.3:-46646911 MS.gene66785:CDS
TAAGGTGGGCTAAGCATGGA+AGG 0.576123 4.3:-46647731 MS.gene66785:CDS
CTTGAAACAGGAGCCCTGAT+GGG 0.576934 4.3:-46645966 MS.gene66785:intron
GATTGCTGAGAAACCTATCT+GGG 0.577637 4.3:-46649075 MS.gene66785:CDS
AAGGTATATTGCATCTGGGT+CGG 0.577707 4.3:-46647712 MS.gene66785:CDS
AATATAGACGTCCTGATGGA+AGG 0.577756 4.3:-46645540 MS.gene66785:CDS
GTATCTTTAGCAGATGAGCT+AGG 0.578133 4.3:+46644347 None:intergenic
TTCTTATTAGGTTCAACTTG+AGG 0.578528 4.3:+46645643 None:intergenic
TGTGTAAGGTGGGCTAAGCA+TGG 0.578561 4.3:-46647735 MS.gene66785:CDS
AGTCTTGCTCACAGGACAGA+TGG 0.579756 4.3:-46646903 MS.gene66785:CDS
CACCACCAGTAGCAAACCTA+AGG 0.581188 4.3:+46649003 None:intergenic
GAACCTAATAAGAAGAGTGA+TGG 0.581485 4.3:-46645634 MS.gene66785:CDS
TGGTGCTCAGAAGACACGAC+AGG 0.582497 4.3:+46645433 None:intergenic
AAGAGTGATGGTCCCGTTGG+TGG 0.582685 4.3:-46645622 MS.gene66785:CDS
AGTACCTTAGGTGGAGCACC+TGG 0.583432 4.3:-46645364 MS.gene66785:CDS
GAGAGGGCACACTGCAGATG+TGG 0.583609 4.3:-46647583 MS.gene66785:intron
CTCTCTATTAGATTAGTGAC+AGG 0.585196 4.3:+46639723 None:intergenic
TGAAGTCTCTGCCTGCCGGA+TGG 0.585315 4.3:-46646493 MS.gene66785:CDS
GGCTGCAGATCCCATCATCA+TGG 0.586799 4.3:+46644499 None:intergenic
CCATGCCAGTTGGAGGTCCA+AGG 0.586990 4.3:+46641397 None:intergenic
GGTACCACAGAATTCGGTAG+TGG 0.587847 4.3:-46647648 MS.gene66785:CDS
AAGAAGAGTGATGGTCCCGT+TGG 0.588017 4.3:-46645625 MS.gene66785:CDS
ACTCATATTCCATATATGGA+TGG 0.589286 4.3:+46647058 None:intergenic
ATCGGATAAGAGTTTGGCAT+GGG 0.590269 4.3:-46641361 MS.gene66785:CDS
GCAATGCCTACCATGATGAT+GGG 0.595359 4.3:-46644509 MS.gene66785:CDS
CAAGCAAACACCAAGCAAGA+AGG 0.596117 4.3:-46645755 MS.gene66785:CDS
TGCCCCTGTCCTCGAGAGAG+GGG 0.596242 4.3:-46646618 MS.gene66785:CDS
AGCTGGCAAAATTTGATGCA+GGG 0.597004 4.3:+46642691 None:intergenic
AGAAGATTTACACAAAGTGT+GGG 0.598390 4.3:-46644538 MS.gene66785:intron
CAAACTACCACTGGCCAATG+TGG 0.600599 4.3:+46647088 None:intergenic
TCACAGGACAGATGGTCACT+GGG 0.601079 4.3:-46646895 MS.gene66785:CDS
AGTCGCTACGGTGATGTGAG+AGG 0.601594 4.3:-46645835 MS.gene66785:CDS
AAAGTCGCAATGACTTTGAG+AGG 0.603804 4.3:-46647600 MS.gene66785:CDS
GTAGCAAACCTAAGGCCACC+AGG 0.606604 4.3:+46649011 None:intergenic
AACTATCCTAATCGAAAGAG+CGG 0.608196 4.3:+46645189 None:intergenic
CCGGAACCTTCTCAATCACA+AGG 0.610220 4.3:+46645281 None:intergenic
ATGCTGTTAATGACATTGCA+GGG 0.610409 4.3:-46645108 MS.gene66785:CDS
GCAGATCCCATCATCATGGT+AGG 0.610628 4.3:+46644503 None:intergenic
GTACATAGGACAATAGGCAT+TGG 0.611246 4.3:+46641856 None:intergenic
ACACAATGCTCCAATTATTG+TGG 0.611428 4.3:-46646567 MS.gene66785:CDS
GGTTCCATCTCTGCCAGAAG+AGG 0.613002 4.3:+46645258 None:intergenic
TGTAGCAACCGATCCATCCA+AGG 0.615103 4.3:+46645924 None:intergenic
CATCGGATAAGAGTTTGGCA+TGG 0.615910 4.3:-46641362 MS.gene66785:CDS
TGTTAATGACATTGCAGGGG+TGG 0.616105 4.3:-46645104 MS.gene66785:CDS
GGGAAATGCATCCATGATGA+GGG 0.616515 4.3:-46645083 MS.gene66785:CDS
CATGGCTGAAGAAACATCAT+CGG 0.616564 4.3:-46641379 MS.gene66785:CDS
ACAAAATTACAACCTCTGCA+CGG 0.617275 4.3:-46645588 MS.gene66785:CDS
GTCACTAATCTAATAGAGAG+TGG 0.618560 4.3:-46639720 MS.gene66785:CDS
CCTTGCAAGAAATCGTACAT+AGG 0.619664 4.3:+46641842 None:intergenic
AATACATACCTAGTCCAACC+TGG 0.620825 4.3:+46642591 None:intergenic
GTTAATGACATTGCAGGGGT+GGG 0.621176 4.3:-46645103 MS.gene66785:CDS
GCAATAACATTGTATGGCTG+TGG 0.621422 4.3:+46646437 None:intergenic
ATGGAAGGTATATTGCATCT+GGG 0.621600 4.3:-46647716 MS.gene66785:CDS
GAGCTGAAGAGAAGTCGCTA+CGG 0.622583 4.3:-46645847 MS.gene66785:CDS
GATAGTTGACAAGAAAGGTG+GGG 0.622735 4.3:-46645173 MS.gene66785:CDS
GTCTCCTGATGATTCCACAT+TGG 0.623387 4.3:-46647102 MS.gene66785:CDS
TGAGGATTCAATATACAGCA+TGG 0.623908 4.3:-46639667 MS.gene66785:CDS
AGACCCAAAATGATCCCGTA+GGG 0.626036 4.3:+46647764 None:intergenic
CAGACCATTCCCCTCTCTCG+AGG 0.627604 4.3:+46646609 None:intergenic
GAGCACCAAAGGGGTAACAA+TGG 0.629150 4.3:-46645418 MS.gene66785:CDS
TACTGGTGGTGGTGACCACA+AGG 0.629423 4.3:-46648991 MS.gene66785:intron
AGTTGACAAGAAAGGTGGGG+AGG 0.629636 4.3:-46645170 MS.gene66785:CDS
GTAAGAACAAGTACCTTAGG+TGG 0.632052 4.3:-46645373 MS.gene66785:CDS
GATACACCAGATCAGGAATG+AGG 0.633367 4.3:-46639561 None:intergenic
GCAAACACCAAGCAAGAAGG+TGG 0.637877 4.3:-46645752 MS.gene66785:CDS
GGATCCCAAAGCCAAAGAAG+AGG 0.641838 4.3:+46641893 None:intergenic
CTCTCCACTACCGAATTCTG+TGG 0.645556 4.3:+46647644 None:intergenic
CAGACCCAAAATGATCCCGT+AGG 0.647447 4.3:+46647763 None:intergenic
TTCCCCTCTCTCGAGGACAG+GGG 0.655046 4.3:+46646616 None:intergenic
AAGTCGCAATGACTTTGAGA+GGG 0.657167 4.3:-46647599 MS.gene66785:CDS
AGGTGATATTAATTCATGAG+AGG 0.657420 4.3:-46647683 MS.gene66785:CDS
GCCTGCCGGATGGTCCAATG+GGG 0.657772 4.3:-46646483 MS.gene66785:CDS
ATAGACGTCCTGATGGAAGG+AGG 0.658288 4.3:-46645537 MS.gene66785:CDS
GATGATTCCACATTGGCCAG+TGG 0.661400 4.3:-46647095 MS.gene66785:CDS
AGTTTGGGCTCTATTCAAAG+TGG 0.661830 4.3:-46642663 MS.gene66785:CDS
ACAGCATGGACAATAACACT+CGG 0.663001 4.3:-46639653 MS.gene66785:CDS
ATTTGCACAACTGTTCTAAG+GGG 0.663592 4.3:-46647029 MS.gene66785:CDS
GGTGCTCAGAAGACACGACA+GGG 0.668430 4.3:+46645434 None:intergenic
GCTTATGTTGATACACGTGT+TGG 0.671428 4.3:-46645697 MS.gene66785:CDS
AGGGCTAGAAATCCGTGCAG+AGG 0.675302 4.3:+46645576 None:intergenic
GCATTGGCGATATTCATTAG+GGG 0.683274 4.3:+46641872 None:intergenic
CAGCATGGACAATAACACTC+GGG 0.683679 4.3:-46639652 MS.gene66785:CDS
CATGGACAATAACACTCGGG+TGG 0.702532 4.3:-46639649 MS.gene66785:CDS
ATACGTAAATGACACTCCTG+AGG 0.702986 4.3:-46639685 MS.gene66785:CDS
GTGGTGATAAAATGACCACA+TGG 0.706319 4.3:+46646671 None:intergenic
AGATTTAAAAGAACGCTCAG+GGG 0.707981 4.3:-46645332 MS.gene66785:CDS
CTTCTGGAAACCATGAGTAG+TGG 0.708208 4.3:+46646652 None:intergenic
CGACTTGGATGGTCTCCATG+TGG 0.712770 4.3:-46646686 MS.gene66785:CDS
CAAATGAAGAGTCTTCTCAG+AGG 0.719314 4.3:-46647797 MS.gene66785:CDS
GTGTCTTCTGAGCACCAAAG+GGG 0.723995 4.3:-46645427 MS.gene66785:CDS
CTCAGAAATCGTAGCCCTAG+CGG 0.727943 4.3:+46645306 None:intergenic
AAAGCATTTGTCTAACACGG+AGG 0.742152 4.3:-46646519 MS.gene66785:CDS
TGCTGTTAATGACATTGCAG+GGG 0.744856 4.3:-46645107 MS.gene66785:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.3 gene 46639563 46649099 46639563 ID=MS.gene66785
chr4.3 mRNA 46639563 46649099 46639563 ID=MS.gene66785.t1;Parent=MS.gene66785
chr4.3 exon 46648992 46649099 46648992 ID=MS.gene66785.t1.exon1;Parent=MS.gene66785.t1
chr4.3 CDS 46648992 46649099 46648992 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46647584 46647871 46647584 ID=MS.gene66785.t1.exon2;Parent=MS.gene66785.t1
chr4.3 CDS 46647584 46647871 46647584 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46646887 46647138 46646887 ID=MS.gene66785.t1.exon3;Parent=MS.gene66785.t1
chr4.3 CDS 46646887 46647138 46646887 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46646332 46646729 46646332 ID=MS.gene66785.t1.exon4;Parent=MS.gene66785.t1
chr4.3 CDS 46646332 46646729 46646332 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46644958 46645978 46644958 ID=MS.gene66785.t1.exon5;Parent=MS.gene66785.t1
chr4.3 CDS 46644958 46645978 46644958 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46644345 46644555 46644345 ID=MS.gene66785.t1.exon6;Parent=MS.gene66785.t1
chr4.3 CDS 46644345 46644555 46644345 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46642600 46642840 46642600 ID=MS.gene66785.t1.exon7;Parent=MS.gene66785.t1
chr4.3 CDS 46642600 46642840 46642600 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46641843 46641965 46641843 ID=MS.gene66785.t1.exon8;Parent=MS.gene66785.t1
chr4.3 CDS 46641843 46641965 46641843 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46641347 46641461 46641347 ID=MS.gene66785.t1.exon9;Parent=MS.gene66785.t1
chr4.3 CDS 46641347 46641461 46641347 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
chr4.3 exon 46639563 46639910 46639563 ID=MS.gene66785.t1.exon10;Parent=MS.gene66785.t1
chr4.3 CDS 46639563 46639910 46639563 ID=cds.MS.gene66785.t1;Parent=MS.gene66785.t1
Gene Sequence

>MS.gene66785

ATGATTGCTGAGAAACCTATCTGGGTTAGGCATGAAGGCATGCAAATTTTCTCCATTGATGTTCAACCTGGTGGCCTTAGGTTTGCTACTGGTGGTGGTGACCACAAGGTATGTTCTTTTTTTCTCATTCTTCTTGTTTTGTTTGAGATTCTCAGTTTATTGCTTAATTTGAATTTTGGGTCTGTTTTTATATAGTTTTGTTGTGTTTGATTTGATATGGTAGCGGATGAGTTAGGTTTGTGCCATTTTGTATGTAATGTTGCATATGAGGCTCGGATACGTAGTACGAGTGCGATATGATACATATATGCAGATAACTAAACGTTTTGAAATTCAGGATATGATACGGCCGAGATACTTTGGCAATTTCTTTTTTTAAAGAAATTATATAATACTTGTAAAAACAGAGTCATTGATCATTCTTCATTCATATACAATTATGATGATCACAATTTTTGTAATATGTTTTAGCTCCTATGTTACGATTTCAACGAAAGCATCATTAAAAGGACTTGAACAAGTATCGACCCTAGACAATAGTAAACAATTCAAAAGCATCTTTAATAAATTATTGTCTAATACTAACAAATTCAGTCTCTTTCCCACATTACTATTGTCCTTAAGAAGCATGTGTTCCGAGTGTGGTTCATCAAAAGACAACTTTGTCAATTCAAGATTCAAGAAGTCCATTGATGTCCGACAACTCTGTCTCATCTTTTTATACCCTCCTAGGATACATATCTGTGTGTATCGGTGAAATATCATATATGTATCTGATTTTTTGTTTATAAAAAAGTAAAACATTCATATACTTCTAGGGTGCATATCTGTGTGTATCTGCAGAGTACCGGTATCCAATATTTATCTGATACGGGTAAATCTCCAATTTTGGAGTATCCAGGCTTCATAGATTTTTGCCTATGGAGTACTGACACGAACACCAAACATGACACTGACACGAACACCAAACATGACACTGACAATGGTAATAATTTGAGAAAATGGTATAATTGAATGTAACGACATGTGTAAGGGCACCGACACATGTCGGATATCGAACACACCTTTAATCTGAAGTGTTGGTGTTGTATATTACTGCTGGTCTCAATGGGAAAAAGAGTGTTGGACAGATGTTTAATGCTTTGTTAGTTAGGTAATAGGAGATTGAGACTGAGAGATATGGTATTGATTTTGGAAAAATCATCCTCTAAACTAAAATGTTTTGCAGGTTCGTATATGGAATATGAAGTTTGTTTCTACTGACTTAACAAATGACTTAACAAATGAAGAGTCTTCTCAGAGGCTTCTTGCAACCCTACGGGATCATTTTGGGTCTGTCAACTGTGTAAGGTGGGCTAAGCATGGAAGGTATATTGCATCTGGGTCGGACGATCAGGTGATATTAATTCATGAGAGGAAGCCTGGTTCAGGTACCACAGAATTCGGTAGTGGAGAGCCACCGGATATTGAAAACTGGAAAGTCGCAATGACTTTGAGAGGGCACACTGCAGATGTGGTAATTTAATTGCTTTTAACCTCATGTCCTTTCTAAAAAGTCTTTATAGTTCCAGTTAACTGTCATTTTCTCTTTGAATGTAAAAGTGTGTTTGGATGGAGGATTTTGGAGGGGAGGGGGAGGGGAGAGGAGTACTTGTTTTTCTTTTCTAAATTATGGAGGATATAAAATGTTTTTAAAGATAAATCAAGTTTGATTGAAATGTTATATGATCATCTATCATGTCATTATTTCAATTTTTAAAAAATCTCAAATCTCAAATATATAACAAAATAGACTTAGCCATCTAAAATCTTCAATCCGAACATACCCTAAATAAACCAGATATTCCATTTAAATGATTCTCTTATCGCTTTTTGTTTTAATCATCACCTTGAGCATTCAACTTCACAGTTTTTTTATCAGCATCATTAACTTGCGATCTACTTGTTCATGTGTAAAGTAGGTGGATCTTAATTGGTCTCCTGATGATTCCACATTGGCCAGTGGTAGTTTGGACAACACCATCCATATATGGAATATGAGTAATGGCATTTGCACAACTGTTCTAAGGGGCCACTCTAGCTTGGTTAAGGGCGTTGCTTGGGATCCTATTGGATCTTTTATAGCAAGTCAATCTGATGATAAAACAGTCATTATCTGGAAAACTAGTGATTGGAGTCTTGCTCACAGGACAGATGGTCACTGGGCTAAATCAGTAAGTTTCTTACTTTGAAGTCTAATAATACTATGCAGCACTGAAACTTCACATTGATGGCGTGTTCAGTGTCCGACATCGATACACGTGGTTACATTCAATTATCCAATTTTATCAAATTATTACTGATATAATTTCTATTTCCTACACCTTTCAGCTTGGATCAACCTTCTTCAGACGACTTGGATGGTCTCCATGTGGTCATTTTATCACCACTACTCATGGTTTCCAGAAGCCAAGGCATTCTGCCCCTGTCCTCGAGAGAGGGGAATGGTCTGCAACTTTTGACTTCTTAGGACACAATGCTCCAATTATTGTGGTCAAGTTCAACCATTCAATGTTCAAAAAGCATTTGTCTAACACGGAGGAAATGAAGTCTCTGCCTGCCGGATGGTCCAATGGGGCTTCCAAGACTGGAAATAAAGAGCCACAGCCATACAATGTTATTGCAATTGGAAGTCAGGATCGAACTATAACTGTTTGGACTACTGCAAGTCCTCGTCCTCTTTTTGTGGCCAAGCACTTTTTTACTCAAAGTGTTGTGGATTTATCCTGGTATGCTATTGTTCTATGGATGCCAGTTTTTTCAACCGCGGATAGCAGAAAGTAGTGGTTTGTTCAAATTACGCTATGCTACAATGCTATAGCGCTGCTATATCCGCTATGTGACACTACTTTTTAGTTTTTACTAAATCGTGTACTGCGGGACAATATCGGATTGTTAAATTCCTGTTATGCTATAGCGCTGCAGTGCCGCTATAACCCCGCTATCTGACAACACTGATGGATGCATCATATGTGTAGTTTGACATTGAATGCTGTTATTTTTTTATTTTTTTGTTAATTAGTTAAAATTATTTTTTGATAATAATTTCTATTATTTATTTATTTATTTCATCTTGAAACAGGAGCCCTGATGGGTATTCACTTTTTGCCTGTTCCTTGGATGGATCGGTTGCTACATTTCATTTTGAGGCGAAAGAACTTGGTCAGAGACTCAGTGATACTGAGTTAGATGAGCTGAAGAGAAGTCGCTACGGTGATGTGAGAGGTCGTCAAGCAAACTTAGCAGAGAGTCCGGCACAGTTATTGCTAGAAGCAGCTTCTACCAAGCAAACACCAAGCAAGAAGGTGGTTGCTAATCCGCAAAACCAGACAATTGCAAAAGCTTATGTTGATACACGTGTTGGTGCAAAAAGTGTTGGTGCAAAAAATGCGGAGCCTCAAGTTGAACCTAATAAGAAGAGTGATGGTCCCGTTGGTGGTGATGCATTAAACAAAATTACAACCTCTGCACGGATTTCTAGCCCTGTGAAGCAAAGAGAATATAGACGTCCTGATGGAAGGAGGAGAATTATTCCAGAAGCAGTTGGAGTTCCTGTTCAGCAGGAAAATATTTCTGGTGCAGTTCAATCCGAAGAACTTGGCTTCCCTGTCGTGTCTTCTGAGCACCAAAGGGGTAACAATGGAGTTGTATTTAACAATGATACCGTAAGAACAAGTACCTTAGGTGGAGCACCTGGCAGAAATTCAGATTTAAAAGAACGCTCAGGGGTTACCGCTAGGGCTACGATTTCTGAGAGCCTTGTGATTGAGAAGGTTCCGGCCTCTTCTGGCAGAGATGGAACCATCAATGTTGAGCAGTTGGGAAATTCGACGACTACTGGTTCTCATGCCGCTCTTTCGATTAGGATAGTTGACAAGAAAGGTGGGGAGGATACTTTACCTATTTGTTTAGAAGCAAGGCCGAGAGAACATGCTGTTAATGACATTGCAGGGGTGGGAAATGCATCCATGATGAGGGAAACAGAAATTGCTTGCACTAGAGGGCCTCAAACACTTTGGTCTGATAGGATATCAGGAAAAGTCACTGTTTTAGCTGGAAATGCAAATTTTTTGGCAGTTGGTTGTGAGGATGGATGCCTGCAGGTATTTAGTCTTTCTATGTGTGTGTATATATGGTTTACTTTTCAATTTCCACTGCTTTTAATTCTGTTAACTATTTTGATATATCGTAAATGTTATTGTATTGTATCATTCATTACCTTTTGTAGTTGCATATTATTTCATCTTTTATTCTGAATTTCCATCATTTATTAAGAAAAACACTTTTAGATACTGAACCATCTCTCTCAATTTCAAAACTCACTAAGAAGTTTCAATTTTGAGATGAGCTTTTGTATTCCATTGACAGATGACTAATTTATGCATGACGTGTGATGTGGGATTCATTACTTTTTGACACTTGTAAAACTATGGCATGATTTATATTCCTATGATGTAGAACATACATTTCATAATGTAATTTGACTAATTTGTGCTGTTGAGAAGATTTACACAAAGTGTGGGAGACGAGCAATGCCTACCATGATGATGGGATCTGCAGCCATATTTGTAGACTGTGATGAAAGTTGGAAGCTGTTGCTGGTGACTAGAAAAGGATCACTTTATTTATGGGATTTATTCAATCGAACCTGTCTCCTTCAAGACTCGTTGGTTTCTCTTGTTGCTTCAAGTCCTAGCTCATCTGCTAAAGATACAGGTAGGTACCATAAATTACCTACATGTTTCATATAATCTCTATCTCTGTTTGCTAAAAATTAGCGAATAAGCTAACTGGTTGAATTTGAAGTGTTTGGTAATATTAGCAGTTGAACTAGTTGATAAATATAAAATCACATAGAAAAATATTTTTAATTGATATTTGATTTTTGTTAATTAGGATAATGAGGGTAAAAATGAGATAAAAATGATACAATATAAGCTATAAGGTCAAACGCTGTTTGAAGTAGCATCTCAAAAAATCCAAAAAGTTCATAAGAGAATAATGTTACCAACCAGTTATACCATACTCGCAATTCACTTTGGCAGTGGTACGGCTACGCAATAAACAACTCTTGATGTACTGGGTGCGTGGGGGATATAAATAGTCGATACATTATTAGATTTCATGGCACATTCTAATAGTTTAATCGGTATGCTATCAATAATTTTGATTTAGGCTGGAAGGTAAAGAAGACTTGATCTAATATTTATGTAAGTTGTTTTTAAAAGAATTATCAATACTAATTTGATTAACAATTTAACAATTATAAAATAAACAAAATATAACCCAGCAGTTAGGAATAATACTCCCTCCGTATCAAAATGAATGTCGTTTAAAGTTTTTACACACATATTAAGAAATGCATTAATTAAAAGAAAGAGAGATGTTATTTTACCAAAATATCCTAATCAACTATTGGTTTGTTTTTCATTAAAGAAAAAATAATACATTGAAGGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGGAAACTGAAAGTGACATTCATTTTGAAACAATTTTTTTTGACTAAAACGACACCCATTTTAAAACGGAGGGAGTATAATATGATTAACCGCATCGTGTGCAACAGTGTGTAACATAGTCTCTTTGGTTTAAGCTTTCATACCAACAAGTTATAATAGTTCTGCACTTCTGCATCGTAAGTGTATAAGAAATAGAGGTAGATTTAGCTACAAAAGAGGGTGTATAACCTGTGTGTTATTTCCTTCCGGAGAACGCTGCTAGTTGTGAATAGCATTGTTTCCTTTATCCGGAAAATTGATTTTAGTGGTTTCAAGGCTTGTTTATAACCGGTTCTAGTGGCTTTAGAGCCAGTTTAATGTTAGAGCTATTTTCATGCTCATTCAAATCAGAGATTGTTGTGTCTCTCTGGTACTTCATTTCCAGCAAATTATAGATGAATTGGTGCATATTAACCAATCTGCAAAAGTATCGGGAATCGGTTCTCACGAACAAATTCATGGTTTTCCACCCACCCCAGCGAATTATGTGACAATGAGTTATACAAGCTTATCTAAGCTATCTTCTATAGGCTAGTAGTTTGGCTTTGCCATACAGAGCTGCCTGAAATTTACAAATTTGTCAGAGCTTATCATTTTTTTATGAGGGCAACTGGTTTTGTTTTGCCAATAAGTTTTTAGATGCTTTGGCTCTATCTCCTTTTATTTTTATTGTATGTATATTTTTAATTCCAGGCACCATTAAAGTTATATCTGCAAAGCTATCAAAGTCTGGTTCTCCTCTTGTTGTTTTGGCAACTCGTCATGCTTTCATATTTGACATGAGTCTCAAGTGTTGGTTAAGGGTTGCTGATGACTGCTTCCCTGCATCAAATTTTGCCAGCTCTTGGAGTTTGGGCTCTATTCAAAGTGGTGAACTGGCTGCTTTGCAGGTCGATGTTAAAAAATATTTGGCCCGCAAGCCAGGTTGGACTAGGTATGTATTTATTGGTATCCATGTTTGACACAGCCTTTAGTTAGTATTTGTCTTCTCTTTGAAAATACTGTTAAATATTGTATGAAATTGATTTAAGGGATATAAGTCATTCTTAATCCTGTAAATTTGTCATAGTGGGCTTTAGTTTAATTTTAGATATGTTCATTAAAATTTCTGAAGATTCAGATATGTATGTAAGCAAGCTAAATATGTATTAAAATAAAAAGATGTCCAAACATACATGAGCATTTAAATATATATGATTGTTGTTTTTGTGTTCCTATTTTCTATCGCAAGTTACATGTTTTAAGTACCTTGGGCCCATCATACAAATTGATGGGGAGATAGTGGGATATTCTTACTTCGCGGTTCTAGTGGGTTGGAAATGGATTGTAATTTGTGAAATTTAATATTCAAGCTCAAAGGTAAGTTAATAGGCTTATTGAGGGAGTTCTACATTGAATGGTTTATATGTAGTTATGGTTTATGAGAGATCTTTATAGTCGAAAAATACTTTTTTGTTCATATTTTTGTTTTGTAGGAATATACAAATCATACTTCTTCTCTAGTGCTCGTCTCACTAGTATATTGATGTTTAAACTAAAAGTTTGTAAACTTGAACTTTTTGGTATAGAATGACTGATGACGGAGTGCATACACGTGCTCACTTGGAAGCTCAGTTGGCCTCTTCTTTGGCTTTGGGATCCCCTAATGAATATCGCCAATGCCTATTGTCCTATGTACGATTTCTTGCAAGGTATTATTTCTGTTTTCTTTTATGATTCTCCAAATGCAATCTAGGCACCTTCTTACTCTTGCACGAAACGTGCATGCTGTCCGTCATCCCATCTCCGACGAAAAAAACCTCTTGCTGTCTGTCTCGCAGCTATCGAACTGTCTTTCTCATGAAATTATGGAAACCTTTTATGAGAGGGATGGGGCCTTTGACATTAATCTTCAAAAACTTAATAATCAAGAGTGGTGCCCCGCATACATTTATTCAATTCCTGAGATTGTATTTACCTAGTTATAAATATAGTTTTATACTTTTTTTGTAATTATTGAATCACATCACACTCTGGTCTATGAATTTGGACCATCAGCAGACTAAACAAAAATTATGATGAAATATTTGCAGAGAAGCAGATGAATCTCGATTACGAGAAGTCTGTGAGAGTTTCCTTGGACCTCCAACTGGCATGGCTGAAGAAACATCATCGGATAAGAGTTTGGCATGGGATCCTGTTGTGCTTGTAAGACTATTTTTAAATCCTTATGCGTAATTAATACATATTGTGTGAACTGTGGTTATATTTTTATTTTCTGCTAAAAATAAGTCACCGTAAGAATTACACCGCATTAGAGCTAAATTTTTTCTTGCGTAAGCTGTGGATTGCGTAATTAATACATATTGTGTGAACTGTGGTTATATTTTTATTTTCTGCTAAAAATAAGTCACCGTAAGAATTACATCGCATTAGAGCTAAATTTTTTCTTGCGTAAGCTGTTGATTACAACCGAAATGGTATATTGGATTACAACCGAAATGGTATATATTTAGAATCAAATTCTTTACCCCAGAGTTTACAACACTTGCCCCTGCGCACCAATTGCGCTAGACCCCAGTGGTAACTGAAATGATACTTGAATGTATGATGGTAGAAATATTGAATAATTCAGTTGGCTGATTATTTAGATGATCTGGCAGTACCAGTATCTTAAGATGCATTATGAAGCACGGACACCTCTAGGATTAGGCGTGTCGCGGTGTCTGACACACGTCAGTGTCCGACACTCCTATGACACTTACGACACGTGTCGGACAAGTGTAGTGTCCTAAGTAAGTTGTTTTCTTCTTCACTTTGACACTCCTTGGACTAGTGTCTGACACCTATACAACACTTATATGAGGCGTGTCGCACTCAGACAAATATCTAGATTTATTTCTCTACTTTCACACAGTGGATTCAAGAGTTAAAGATGGGCGAGAAATGTTAATGAAATAAATTGCTCAAATTAGATGCATATGTATCTATTTTGTTCTTAATTTAGATCTTTTATAAATAAATTATTATATAGGTAGCGGTGTCAGTGTCCTATGTTTTTACATTAGCGGTGTCGGCGTGTCCGTGTCAGTGTCAGTGTCAGGTGTCCGTGTCGGAGTCCGTGCTTCATAGATGAGAATCATATCTTGATATGATACTAAACTGACTCAATCGATACAAATCTTTTGTGTGTATCAATTTTGGATACGAATCTCATGATGAATCCCAATTCTTTATGATGAATCCCAATTCACTATGATGAATCTTGATTCATTCTAATGAATCACTACCTAAACTTGTATAGTCAACCACTTTTTTTTTGGCAACTTTGTAAAAACTATACCCTTTTGTTTTGTCATTTAATTAATGACTTACATCTTGAATTACTTTGAAGGTCATATATGATATACCAGGTTATAGTTTTGTTCCCTAAAAAAAAGGATATAGTTTTGTTATGTCACTTGTTTTTTTTACTTAAAATGACATTTCTACTGGCAATGTATCTTACAATTCATAGTAAGATTCAATTCACGATTCGGAAAATAGGTATTCTGATTTGCGATTTGATAGTTATGACCTTGAGTACCAAATCATCACATGTTGAGCTCATCGACTGATCAACTTTCCCATTTGAATCTTTGCAGGGAATGAGGAAACACAAACTCCTTATCGAAGACATTCTTCCAGCCATGGCATCAAATAGGAAAGTCCAGAGACTGCTCAACGAATTCATGGATCTAGTATCCGAATACGAAATTGCTGATACTAATCATGATCAAAGAAATCCAGTGTTACTCAAGACATCTTCACCTGTCACTAATCTAATAGAGAGTGGTCCATTAGGAACATACGTAAATGACACTCCTGAGGATTCAATATACAGCATGGACAATAACACTCGGGTGGTAGAAAGTCGTGAAAATTTCACTATTGCTGCAAATGAAGCTAGTGCACATGATCAAATTGTTCAGGATACACCAGATCAGGAATGA
Protein sequence

>MS.gene66785.t1

MIAEKPIWVRHEGMQIFSIDVQPGGLRFATGGGDHKVRIWNMKFVSTDLTNDLTNEESSQRLLATLRDHFGSVNCVRWAKHGRYIASGSDDQVILIHERKPGSGTTEFGSGEPPDIENWKVAMTLRGHTADVVDLNWSPDDSTLASGSLDNTIHIWNMSNGICTTVLRGHSSLVKGVAWDPIGSFIASQSDDKTVIIWKTSDWSLAHRTDGHWAKSLGSTFFRRLGWSPCGHFITTTHGFQKPRHSAPVLERGEWSATFDFLGHNAPIIVVKFNHSMFKKHLSNTEEMKSLPAGWSNGASKTGNKEPQPYNVIAIGSQDRTITVWTTASPRPLFVAKHFFTQSVVDLSWSPDGYSLFACSLDGSVATFHFEAKELGQRLSDTELDELKRSRYGDVRGRQANLAESPAQLLLEAASTKQTPSKKVVANPQNQTIAKAYVDTRVGAKSVGAKNAEPQVEPNKKSDGPVGGDALNKITTSARISSPVKQREYRRPDGRRRIIPEAVGVPVQQENISGAVQSEELGFPVVSSEHQRGNNGVVFNNDTVRTSTLGGAPGRNSDLKERSGVTARATISESLVIEKVPASSGRDGTINVEQLGNSTTTGSHAALSIRIVDKKGGEDTLPICLEARPREHAVNDIAGVGNASMMRETEIACTRGPQTLWSDRISGKVTVLAGNANFLAVGCEDGCLQIYTKCGRRAMPTMMMGSAAIFVDCDESWKLLLVTRKGSLYLWDLFNRTCLLQDSLVSLVASSPSSSAKDTGTIKVISAKLSKSGSPLVVLATRHAFIFDMSLKCWLRVADDCFPASNFASSWSLGSIQSGELAALQVDVKKYLARKPGWTRMTDDGVHTRAHLEAQLASSLALGSPNEYRQCLLSYVRFLAREADESRLREVCESFLGPPTGMAEETSSDKSLAWDPVVLGMRKHKLLIEDILPAMASNRKVQRLLNEFMDLVSEYEIADTNHDQRNPVLLKTSSPVTNLIESGPLGTYVNDTPEDSIYSMDNNTRVVESRENFTIAANEASAHDQIVQDTPDQE