AlfalfaGEDB | MS.gene67269

M. sativa cultivar XinJiangDaYe / MS.gene67269

KEGG: -
GO: GO:0000145 GO:0005575 GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0032991 GO:0046903 GO:0051179 GO:0051234 GO:0099023 GO:0140352
PFAM: PF03081.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67269.t1	XP_003615883.1	98.3	644	11	0	1	644	1	644	0.00E+00	1272.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67269.t1	Q9FGH9	69.0	638	177	7	1	635	1	620	2.2e-255	882.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67269.t1	G7JZR5	98.3	644	11	0	1	644	1	644	0.0e+00	1272.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene67269	0.856242	3.67E-62	-1.69E-46
MS.gene04917	MS.gene67269	0.856335	3.45E-62	-1.69E-46
MS.gene049685	MS.gene67269	0.821737	3.44E-53	-1.69E-46
MS.gene04998	MS.gene67269	0.833311	5.74E-56	-1.69E-46
MS.gene050114	MS.gene67269	0.826367	2.82E-54	-1.69E-46
MS.gene050317	MS.gene67269	0.808775	2.62E-50	-1.69E-46
MS.gene050359	MS.gene67269	0.80127	9.75E-49	-1.69E-46
MS.gene050386	MS.gene67269	0.808816	2.57E-50	-1.69E-46
MS.gene050654	MS.gene67269	0.805684	1.19E-49	-1.69E-46
MS.gene050941	MS.gene67269	0.802349	5.85E-49	-1.69E-46
MS.gene051184	MS.gene67269	0.840808	6.96E-58	-1.69E-46
MS.gene051809	MS.gene67269	0.801809	7.56E-49	-1.69E-46
MS.gene052342	MS.gene67269	0.800596	1.34E-48	-1.69E-46
MS.gene056097	MS.gene67269	0.841312	5.13E-58	-1.69E-46
MS.gene056181	MS.gene67269	0.804965	1.68E-49	-1.69E-46
MS.gene056752	MS.gene67269	0.847915	8.55E-60	-1.69E-46
MS.gene056787	MS.gene67269	0.800615	1.33E-48	-1.69E-46
MS.gene056849	MS.gene67269	0.828557	8.41E-55	-1.69E-46
MS.gene056992	MS.gene67269	0.810728	9.98E-51	-1.69E-46
MS.gene057452	MS.gene67269	-0.850076	2.14E-60	-1.69E-46
MS.gene057999	MS.gene67269	0.822479	2.32E-53	-1.69E-46
MS.gene058278	MS.gene67269	0.825478	4.59E-54	-1.69E-46
MS.gene058758	MS.gene67269	0.819694	1.02E-52	-1.69E-46
MS.gene059007	MS.gene67269	0.819942	8.91E-53	-1.69E-46
MS.gene059050	MS.gene67269	0.802424	5.65E-49	-1.69E-46
MS.gene060259	MS.gene67269	0.817474	3.24E-52	-1.69E-46
MS.gene060563	MS.gene67269	0.835497	1.62E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67269.t1	MTR_5g073450	98.292	644	11	0	1	644	1	644	0.0	1315
MS.gene67269.t1	MTR_3g031130	69.457	645	177	5	1	642	1	628	0.0	865
MS.gene67269.t1	MTR_5g073460	64.574	446	156	2	194	637	251	696	0.0	586
MS.gene67269.t1	MTR_5g073480	57.666	437	119	5	194	627	252	625	5.25e-165	486
MS.gene67269.t1	MTR_5g073620	62.575	334	105	2	194	525	280	595	2.04e-131	413
MS.gene67269.t1	MTR_5g073620	39.147	258	135	4	378	634	833	1069	5.67e-40	157
MS.gene67269.t1	MTR_8g021330	41.667	456	254	5	201	644	161	616	9.08e-124	380
MS.gene67269.t1	MTR_4g103540	34.294	659	393	10	6	634	33	681	3.89e-117	365
MS.gene67269.t1	MTR_6g033310	36.746	547	309	10	114	644	212	737	8.82e-113	355
MS.gene67269.t1	MTR_7g013770	33.683	668	388	14	6	634	16	667	4.98e-111	349
MS.gene67269.t1	MTR_7g026630	37.989	537	308	10	119	643	137	660	2.56e-110	347
MS.gene67269.t1	MTR_5g074130	44.866	448	225	9	190	631	242	673	2.24e-108	344
MS.gene67269.t1	MTR_1g057560	32.647	631	395	9	22	641	22	633	4.27e-108	340
MS.gene67269.t1	MTR_1g090620	30.864	648	399	10	22	641	23	649	2.18e-105	333
MS.gene67269.t1	MTR_1g117090	33.445	598	336	12	91	642	96	677	3.83e-95	307
MS.gene67269.t1	MTR_5g073920	39.644	449	237	9	196	637	242	663	7.15e-92	300
MS.gene67269.t1	MTR_3g025900	31.303	591	356	13	70	637	49	612	4.09e-87	285
MS.gene67269.t1	MTR_3g464010	39.732	448	242	11	196	635	243	670	1.31e-82	274
MS.gene67269.t1	MTR_2g096230	28.933	553	355	9	94	640	68	588	3.00e-82	271
MS.gene67269.t1	MTR_5g093530	43.324	367	172	5	267	631	238	570	2.97e-81	268
MS.gene67269.t1	MTR_4g062330	30.741	553	346	12	94	631	72	602	1.91e-77	259
MS.gene67269.t1	MTR_5g073900	44.072	388	171	10	219	603	1	345	1.03e-74	243
MS.gene67269.t1	MTR_5g073770	64.550	189	65	1	192	378	191	379	1.95e-74	244
MS.gene67269.t1	MTR_1g067480	30.153	524	326	12	125	631	100	600	1.87e-71	242
MS.gene67269.t1	MTR_1g067460	28.681	523	336	10	125	631	98	599	1.72e-65	226
MS.gene67269.t1	MTR_5g073490	53.846	169	63	1	467	635	3	156	5.38e-56	187
MS.gene67269.t1	MTR_5g073820	42.321	293	76	5	192	481	230	432	2.93e-52	187
MS.gene67269.t1	MTR_5g073960	49.758	207	76	6	177	378	233	416	5.19e-45	166
MS.gene67269.t1	MTR_5g073780	39.669	242	124	4	394	634	4	224	4.57e-43	155
MS.gene67269.t1	MTR_1g009620	26.296	521	333	10	131	642	184	662	4.85e-38	150
MS.gene67269.t1	MTR_5g074190	34.000	300	120	8	336	632	184	408	1.85e-31	129
MS.gene67269.t1	MTR_5g073650	38.384	198	80	3	200	396	171	327	3.06e-30	122
MS.gene67269.t1	MTR_1g017910	26.077	441	267	12	197	599	236	655	4.25e-30	126
MS.gene67269.t1	MTR_5g073810	38.776	196	78	3	202	396	275	429	5.47e-28	117
MS.gene67269.t1	MTR_4g008260	25.339	442	295	12	205	632	220	640	6.66e-26	113
MS.gene67269.t1	MTR_0229s0040	31.746	189	123	2	458	642	31	217	1.89e-22	97.1
MS.gene67269.t1	MTR_4g019260	45.946	74	34	1	267	340	2	69	4.08e-13	65.1
MS.gene67269.t1	MTR_3g031110	63.333	60	19	1	279	335	17	76	1.58e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67269.t1	AT5G58430	69.279	638	175	7	1	635	1	620	0.0	885
MS.gene67269.t1	AT1G07000	49.767	645	267	10	1	635	1	598	0.0	630
MS.gene67269.t1	AT5G50380	35.000	660	386	11	7	634	24	672	1.55e-119	371
MS.gene67269.t1	AT5G13990	37.990	587	330	14	74	643	124	693	6.31e-119	370
MS.gene67269.t1	AT3G14090	36.280	543	317	7	126	643	85	623	2.79e-118	366
MS.gene67269.t1	AT1G72470	41.394	459	250	7	201	644	178	632	3.88e-117	363
MS.gene67269.t1	AT5G13150	36.986	584	308	14	91	643	99	653	6.42e-113	353
MS.gene67269.t1	AT1G54090	39.735	453	260	6	201	642	170	620	1.72e-112	351
MS.gene67269.t1	AT5G03540	32.701	633	385	9	22	635	22	632	1.92e-108	341
MS.gene67269.t1	AT5G03540	32.675	658	390	11	22	641	22	664	5.68e-107	338
MS.gene67269.t1	AT5G03540	39.229	441	249	4	204	635	87	517	1.07e-105	330
MS.gene67269.t1	AT5G52340	30.941	627	409	6	22	641	22	631	1.69e-105	333
MS.gene67269.t1	AT3G29400	32.340	671	403	16	4	643	7	657	1.25e-94	305
MS.gene67269.t1	AT5G52350	33.693	463	292	4	176	635	129	579	1.31e-84	277
MS.gene67269.t1	AT2G39380	28.080	552	370	7	94	634	76	611	5.68e-77	258
MS.gene67269.t1	AT5G59730	28.618	608	368	14	63	634	22	599	4.16e-74	250
MS.gene67269.t1	AT5G59730	28.618	608	368	14	63	634	22	599	4.47e-74	250
MS.gene67269.t1	AT3G55150	28.364	550	371	8	94	634	76	611	7.58e-72	244
MS.gene67269.t1	AT5G61010	27.134	656	412	15	15	643	23	639	7.74e-72	244
MS.gene67269.t1	AT5G61010	27.134	656	412	15	15	643	23	639	7.74e-72	244
MS.gene67269.t1	AT1G07725	28.172	536	318	10	125	633	96	591	3.86e-71	242
MS.gene67269.t1	AT3G09530	26.384	614	416	9	35	633	10	602	1.35e-68	236
MS.gene67269.t1	AT2G28640	28.298	523	331	8	125	637	94	582	6.51e-67	230
MS.gene67269.t1	AT3G09520	28.164	561	363	10	89	632	68	605	1.50e-65	227
MS.gene67269.t1	AT2G28650	28.842	423	278	5	212	631	152	554	2.65e-52	189
MS.gene67269.t1	AT4G31540	25.775	516	335	9	135	642	190	665	2.23e-39	154
MS.gene67269.t1	AT1G51640	24.494	445	301	10	209	632	196	626	7.97e-25	110

Find 143 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCAGGGTCCTTATAGATTT+TGG	0.108866	5.1:-56728239	None:intergenic
CAGCCATGTTGTTATTATTA+TGG	0.177095	5.1:-56726893	None:intergenic
CGAATGGTTGCCGGTGGATT+TGG	0.193761	5.1:+56727482	MS.gene67269:CDS
TTTCATCAGATCATCCAATT+TGG	0.196368	5.1:+56727075	MS.gene67269:CDS
GGAGCGGTTGTTTAAAATTC+TGG	0.212540	5.1:+56727823	MS.gene67269:CDS
TCCGGCTAAGGCGGGTGTTC+CGG	0.217088	5.1:+56728006	MS.gene67269:CDS
CCGGCTAAGGCGGGTGTTCC+GGG	0.220217	5.1:+56728007	MS.gene67269:CDS
CCTTAATCCATGAGCTTCTT+TGG	0.233529	5.1:-56728396	None:intergenic
GAAATTGAAAGATGGATTAA+AGG	0.249788	5.1:+56727632	MS.gene67269:CDS
AAAACTCAATGATTTGTTTC+AGG	0.259634	5.1:+56728705	MS.gene67269:CDS
TCCAAGAGCTTTCACGGCTT+TGG	0.277854	5.1:+56727010	MS.gene67269:CDS
AGGAGAGTTTGTCGAGATTA+GGG	0.287412	5.1:+56727552	MS.gene67269:CDS
TAAGGTGTTTGGGTTTCTTA+AGG	0.292868	5.1:+56728414	MS.gene67269:CDS
AAAGCCGAAGATAGTGAATT+AGG	0.312797	5.1:+56728307	MS.gene67269:CDS
AATGGATCAATGCAACATAA+TGG	0.314221	5.1:+56728445	MS.gene67269:CDS
AGCTCATGGATTAAGGTGTT+TGG	0.315385	5.1:+56728403	MS.gene67269:CDS
TTTCTTAAGGTGGAGAATAA+TGG	0.326928	5.1:+56728427	MS.gene67269:CDS
CCAAAGAAGCTCATGGATTA+AGG	0.329377	5.1:+56728396	MS.gene67269:CDS
AACATTGCGTGACCTAATTT+CGG	0.332839	5.1:+56727856	MS.gene67269:CDS
GCTGATAAATATATTAAGTA+TGG	0.333613	5.1:+56728664	MS.gene67269:CDS
ACAACTCAAACTCCGAAATT+AGG	0.336754	5.1:-56727868	None:intergenic
AGCAAAATCCAAAATCTATA+AGG	0.337586	5.1:+56728231	MS.gene67269:CDS
ATGGATCAATGCAACATAAT+GGG	0.338830	5.1:+56728446	MS.gene67269:CDS
TACAGATTTAGCTTCGCTTT+CGG	0.343027	5.1:-56728750	None:intergenic
TATCTTTGATGAACAACTTA+AGG	0.343532	5.1:+56728549	MS.gene67269:CDS
GAGCAAGTCTTTGAAGACTA+TGG	0.352862	5.1:+56728103	MS.gene67269:CDS
TCAGCAGAGTCTGCCCAAAT+TGG	0.354285	5.1:-56727089	None:intergenic
TTCATCACGTAACGCGTAAT+CGG	0.355133	5.1:-56728043	None:intergenic
GAGGAGAGTTTGTCGAGATT+AGG	0.359256	5.1:+56727551	MS.gene67269:CDS
AGCTTCTTTGGTACTGCATA+TGG	0.365030	5.1:-56728384	None:intergenic
TCCGTTACCGGCATCGCAAC+CGG	0.366270	5.1:-56727395	None:intergenic
GCTCATGGATTAAGGTGTTT+GGG	0.366445	5.1:+56728404	MS.gene67269:CDS
GGAGCTATTAGAGAGCAATT+TGG	0.368249	5.1:+56728207	MS.gene67269:CDS
TGCTCCTAATTCACTATCTT+CGG	0.368543	5.1:-56728311	None:intergenic
GAATGGTTGCCGGTGGATTT+GGG	0.370483	5.1:+56727483	MS.gene67269:CDS
TTCGTCTTTGTCGGTGCAAA+TGG	0.370914	5.1:+56728174	MS.gene67269:CDS
AAGGCGGGTGTTCCGGGTGG+TGG	0.371274	5.1:+56728013	MS.gene67269:CDS
TTCATCAGATCATCCAATTT+GGG	0.380165	5.1:+56727076	MS.gene67269:CDS
CAGAGATCCAAGAGCTTTCA+CGG	0.381140	5.1:+56727004	MS.gene67269:CDS
TAGTTGCCGGAGAGAATTCT+TGG	0.391094	5.1:+56727529	MS.gene67269:CDS
GGTGCAAATGGATTGGATTA+TGG	0.395788	5.1:+56728186	MS.gene67269:CDS
TGCATCCTATCATCCATCTT+AGG	0.400142	5.1:-56728145	None:intergenic
GCATTCATCTTCGTCTTTGT+CGG	0.400996	5.1:+56728165	MS.gene67269:CDS
ATTACTATATGGAAGAGATT+GGG	0.401127	5.1:+56727932	MS.gene67269:CDS
CTTTGTCGGTGCAAATGGAT+TGG	0.403105	5.1:+56728179	MS.gene67269:CDS
TATTCCGGTACGGCGGAGTT+AGG	0.407125	5.1:-56727295	None:intergenic
ACAATTTCATAATCCGTTAC+CGG	0.411801	5.1:-56727407	None:intergenic
TTAGCTTCGCTTTCGGCTAC+CGG	0.424223	5.1:-56728743	None:intergenic
GGAATGAAGCAATTACTATA+TGG	0.424616	5.1:+56727921	MS.gene67269:CDS
TTCGCAGATGCGGTTGCGAT+TGG	0.427350	5.1:+56727788	MS.gene67269:CDS
TTTCAAAATGTTGCTGAAGT+TGG	0.430084	5.1:+56728634	MS.gene67269:CDS
AAACTCAATGATTTGTTTCA+GGG	0.430768	5.1:+56728706	MS.gene67269:CDS
AATTACTATATGGAAGAGAT+TGG	0.434289	5.1:+56727931	MS.gene67269:CDS
ATCGCGAAACGAATGGTTGC+CGG	0.437422	5.1:+56727473	MS.gene67269:CDS
TCCAAAGCCGTGAAAGCTCT+TGG	0.438932	5.1:-56727011	None:intergenic
CGCATGTGTACAGTAGTTGC+CGG	0.441409	5.1:+56727516	MS.gene67269:CDS
AATTCACCGAATCGTTATTC+CGG	0.449218	5.1:-56727310	None:intergenic
GAATTTGATTCGAAGGGATC+CGG	0.454380	5.1:+56727988	MS.gene67269:CDS
ATTATCCTAAGATGGATGAT+AGG	0.458844	5.1:+56728140	MS.gene67269:CDS
CCCGGAACACCCGCCTTAGC+CGG	0.464875	5.1:-56728007	None:intergenic
TCTGATTCCGGTTGCGATGC+CGG	0.465064	5.1:+56727388	MS.gene67269:CDS
TGCGATTGGAAGCCGGTCGC+CGG	0.467580	5.1:+56727802	MS.gene67269:CDS
ACAATGAAATTTGCATAAGC+TGG	0.471843	5.1:-56728607	None:intergenic
GATTCGAAGGGATCCGGCTA+AGG	0.472839	5.1:+56727994	MS.gene67269:CDS
AGATGAGTTTCGTTCTCTCA+TGG	0.474512	5.1:+56727250	MS.gene67269:CDS
CAGGGAAGCAGCGGATCAAC+CGG	0.475342	5.1:+56728724	MS.gene67269:CDS
TGGTGAAGAGGATCTGATTC+CGG	0.477953	5.1:+56727376	MS.gene67269:CDS
TAAACAACCGCTCCGGCGAC+CGG	0.479945	5.1:-56727814	None:intergenic
CAAATCTTCTCAAATTTCGA+TGG	0.480862	5.1:+56726927	MS.gene67269:CDS
AAGAAAACGTAGCACAAAGC+AGG	0.482301	5.1:-56728256	None:intergenic
GGTGTTTGGGTTTCTTAAGG+TGG	0.482519	5.1:+56728417	MS.gene67269:CDS
CGGTGTTTGATGATTTATGC+AGG	0.484441	5.1:+56728509	MS.gene67269:CDS
TTGAAGATGAAATTGAAAGA+TGG	0.490489	5.1:+56727624	MS.gene67269:CDS
ATTGAAGGATTATCCTAAGA+TGG	0.495216	5.1:+56728132	MS.gene67269:CDS
ACCGTTTGATTCTGAAGAAG+AGG	0.499752	5.1:+56727334	MS.gene67269:CDS
TCCGGTTGCGATGCCGGTAA+CGG	0.500704	5.1:+56727394	MS.gene67269:CDS
ATCTTAGGATAATCCTTCAA+TGG	0.501748	5.1:-56728130	None:intergenic
ATCAGTATAGTGTGTCTTTG+AGG	0.503425	5.1:+56727900	MS.gene67269:CDS
TCCTCTTCTTCAGAATCAAA+CGG	0.503787	5.1:-56727335	None:intergenic
GTGTAGGTCGCGGCAGACTT+TGG	0.505804	5.1:+56728081	MS.gene67269:CDS
AGTTGGAGAATTTGATTCGA+AGG	0.519933	5.1:+56727981	MS.gene67269:CDS
AGTGAGAGATTCGGCGGTCG+AGG	0.522517	5.1:-56727049	None:intergenic
TGAGAAAGTCGCTGCGGTCG+AGG	0.527276	5.1:+56726974	MS.gene67269:CDS
CGAAGGGATCCGGCTAAGGC+GGG	0.530606	5.1:+56727998	MS.gene67269:CDS
ATTACCTTCGTGCTGCGTGT+AGG	0.531020	5.1:+56728065	MS.gene67269:CDS
TCGAAGGGATCCGGCTAAGG+CGG	0.531883	5.1:+56727997	MS.gene67269:CDS
TGATTCTGAAGAAGAGGATG+AGG	0.534419	5.1:+56727340	MS.gene67269:CDS
GCCGTACCGGAATAACGATT+CGG	0.534490	5.1:+56727304	MS.gene67269:CDS
TATGCAGGGTTCAATCTACA+TGG	0.536987	5.1:+56728524	MS.gene67269:CDS
GTTGGAGAATTTGATTCGAA+GGG	0.538931	5.1:+56727982	MS.gene67269:CDS
TGTTCACAAAATGCAGTGGC+AGG	0.547500	5.1:+56727598	MS.gene67269:CDS
TCGCATAATCGTCGCTCACT+CGG	0.551165	5.1:-56727683	None:intergenic
ATGCGGTTGCGATTGGAAGC+CGG	0.551749	5.1:+56727795	MS.gene67269:CDS
TTCGTGCTGCGTGTAGGTCG+CGG	0.551910	5.1:+56728071	MS.gene67269:CDS
ACCGAATCGTTATTCCGGTA+CGG	0.552709	5.1:-56727305	None:intergenic
TCACGAGATCGCGAAACGAA+TGG	0.552800	5.1:+56727466	MS.gene67269:CDS
TTGACCTAACTCCGCCGTAC+CGG	0.553540	5.1:+56727291	MS.gene67269:CDS
CTTATGCAAATTTCATTGTG+AGG	0.557442	5.1:+56728611	MS.gene67269:CDS
ATGAAACGTAGTGAGAGATT+CGG	0.558715	5.1:-56727058	None:intergenic
TTACTATATGGAAGAGATTG+GGG	0.558787	5.1:+56727933	MS.gene67269:CDS
TGGATTTATCACCGGTGAGG+TGG	0.560283	5.1:-56727169	None:intergenic
AGACTATGGACATCCATTGA+AGG	0.560704	5.1:+56728117	MS.gene67269:CDS
TGCAGTACCAAAGAAGCTCA+TGG	0.561080	5.1:+56728389	MS.gene67269:CDS
GATGAGGAAATCGACGGTGG+TGG	0.568555	5.1:+56727356	MS.gene67269:CDS
GTTGTTGAATTTCGCAGATG+CGG	0.569479	5.1:+56727778	MS.gene67269:CDS
TTGGAAGCCGGTCGCCGGAG+CGG	0.578483	5.1:+56727807	MS.gene67269:CDS
GCGTGCAAGACACGTGGCGA+TGG	0.587844	5.1:-56727189	None:intergenic
TCTCCTCCAAGAATTCTCTC+CGG	0.592000	5.1:-56727535	None:intergenic
AATCGACGGTGGTGGTGAAG+AGG	0.592097	5.1:+56727364	MS.gene67269:CDS
CACGTGTCTTGCACGCGCTG+AGG	0.603781	5.1:+56727196	MS.gene67269:CDS
TTGCCGGAGAGAATTCTTGG+AGG	0.606882	5.1:+56727532	MS.gene67269:CDS
GCAGAGTGGAACCACCTCAC+CGG	0.613195	5.1:+56727158	MS.gene67269:CDS
GGTGTTTGATGATTTATGCA+GGG	0.615103	5.1:+56728510	MS.gene67269:CDS
ACACTCTTTCCCAAATCCAC+CGG	0.617998	5.1:-56727492	None:intergenic
AGATCGTTAATCGTCGCTGA+CGG	0.621884	5.1:-56727443	None:intergenic
TGGATCAATGCAACATAATG+GGG	0.624314	5.1:+56728447	MS.gene67269:CDS
TTTCGATGGAAGATTCTCGA+AGG	0.631139	5.1:+56726941	MS.gene67269:CDS
AGAAAACGTAGCACAAAGCA+GGG	0.636456	5.1:-56728255	None:intergenic
CGTGGCGATGGATTTATCAC+CGG	0.638141	5.1:-56727177	None:intergenic
GAGGTGGTTCCACTCTGCAA+CGG	0.639466	5.1:-56727153	None:intergenic
CTCAGCGCGTGCAAGACACG+TGG	0.639873	5.1:-56727195	None:intergenic
GCTAAGGCGGGTGTTCCGGG+TGG	0.643592	5.1:+56728010	MS.gene67269:CDS
GCGAAACGAATGGTTGCCGG+TGG	0.645091	5.1:+56727476	MS.gene67269:CDS
GAAGAGGATGAGGAAATCGA+CGG	0.652395	5.1:+56727350	MS.gene67269:CDS
GATTTGTTTCAGGGAAGCAG+CGG	0.656597	5.1:+56728715	MS.gene67269:CDS
CGTTCTCTCATGGAACGCGG+TGG	0.656643	5.1:+56727260	MS.gene67269:CDS
CGATGGATTTATCACCGGTG+AGG	0.664178	5.1:-56727172	None:intergenic
AGAAGAGAAATTACTCGCTG+TGG	0.664906	5.1:+56726845	MS.gene67269:CDS
ATCGGATGTAGTCCACCACC+CGG	0.665264	5.1:-56728025	None:intergenic
TAGTAGCTTCCGTTGCAGAG+TGG	0.667358	5.1:+56727144	MS.gene67269:CDS
ATATATTAAGTATGGAACAG+AGG	0.672378	5.1:+56728672	MS.gene67269:CDS
TTTCGTTCTCTCATGGAACG+CGG	0.674207	5.1:+56727257	MS.gene67269:CDS
AAGATGTTCACAAAATGCAG+TGG	0.676259	5.1:+56727594	MS.gene67269:CDS
AACGGCGTCGAGAAAAGCAG+CGG	0.676927	5.1:-56727114	None:intergenic
GGAAGCTACTAAGTCGTCAA+CGG	0.681900	5.1:-56727132	None:intergenic
TACTATATGGAAGAGATTGG+GGG	0.699675	5.1:+56727934	MS.gene67269:CDS
AAACGTAGTGAGAGATTCGG+CGG	0.706086	5.1:-56727055	None:intergenic
GAGGATGAGGAAATCGACGG+TGG	0.709462	5.1:+56727353	MS.gene67269:CDS
AGGCCATAATAATAACAACA+TGG	0.709937	5.1:+56726890	MS.gene67269:CDS
GCGACCTACACGCAGCACGA+AGG	0.713813	5.1:-56728069	None:intergenic
TCTTTCTGAGAAAGTCGCTG+CGG	0.726564	5.1:+56726968	MS.gene67269:CDS
GAATCGTTATTCCGGTACGG+CGG	0.733458	5.1:-56727302	None:intergenic
GAAGCTGAAATCATTCAACA+CGG	0.746033	5.1:+56728489	MS.gene67269:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GCTGATAAATATATTAAGTA+TGG	+	chr5.1:56728664-56728683	MS.gene67269:CDS	20.0%
!	AAAAACACACTGCAAAAATT+CGG	+	chr5.1:56728356-56728375	MS.gene67269:CDS	25.0%
!	AATTACTATATGGAAGAGAT+TGG	+	chr5.1:56727931-56727950	MS.gene67269:CDS	25.0%
!	AGCAAAATCCAAAATCTATA+AGG	+	chr5.1:56728231-56728250	MS.gene67269:CDS	25.0%
!	ATATATTAAGTATGGAACAG+AGG	+	chr5.1:56728672-56728691	MS.gene67269:CDS	25.0%
!	ATTACTATATGGAAGAGATT+GGG	+	chr5.1:56727932-56727951	MS.gene67269:CDS	25.0%
!	TATCTTTGATGAACAACTTA+AGG	+	chr5.1:56728549-56728568	MS.gene67269:CDS	25.0%
!	TTGAAGATGAAATTGAAAGA+TGG	+	chr5.1:56727624-56727643	MS.gene67269:CDS	25.0%
!!	TTTTCTTGATGAATAATTGC+AGG	+	chr5.1:56728272-56728291	MS.gene67269:CDS	25.0%
!!!	AAAACTCAATGATTTGTTTC+AGG	+	chr5.1:56728705-56728724	MS.gene67269:CDS	25.0%
!!!	AAACTCAATGATTTGTTTCA+GGG	+	chr5.1:56728706-56728725	MS.gene67269:CDS	25.0%
!!!	GAAATTGAAAGATGGATTAA+AGG	+	chr5.1:56727632-56727651	MS.gene67269:CDS	25.0%
	AATGGATCAATGCAACATAA+TGG	+	chr5.1:56728445-56728464	MS.gene67269:CDS	30.0%
	ACAATGAAATTTGCATAAGC+TGG	-	chr5.1:56728610-56728629	None:intergenic	30.0%
	ACAATTTCATAATCCGTTAC+CGG	-	chr5.1:56727410-56727429	None:intergenic	30.0%
	AGGCCATAATAATAACAACA+TGG	+	chr5.1:56726890-56726909	MS.gene67269:CDS	30.0%
	ATCTTAGGATAATCCTTCAA+TGG	-	chr5.1:56728133-56728152	None:intergenic	30.0%
	ATGGATCAATGCAACATAAT+GGG	+	chr5.1:56728446-56728465	MS.gene67269:CDS	30.0%
	ATTATCCTAAGATGGATGAT+AGG	+	chr5.1:56728140-56728159	MS.gene67269:CDS	30.0%
	ATTGAAGGATTATCCTAAGA+TGG	+	chr5.1:56728132-56728151	MS.gene67269:CDS	30.0%
	CAAATCTTCTCAAATTTCGA+TGG	+	chr5.1:56726927-56726946	MS.gene67269:CDS	30.0%
	CTTATGCAAATTTCATTGTG+AGG	+	chr5.1:56728611-56728630	MS.gene67269:CDS	30.0%
	GGAATGAAGCAATTACTATA+TGG	+	chr5.1:56727921-56727940	MS.gene67269:CDS	30.0%
	TTACTATATGGAAGAGATTG+GGG	+	chr5.1:56727933-56727952	MS.gene67269:CDS	30.0%
	TTCATCAGATCATCCAATTT+GGG	+	chr5.1:56727076-56727095	MS.gene67269:CDS	30.0%
	TTTCAAAATGTTGCTGAAGT+TGG	+	chr5.1:56728634-56728653	MS.gene67269:CDS	30.0%
	TTTCATCAGATCATCCAATT+TGG	+	chr5.1:56727075-56727094	MS.gene67269:CDS	30.0%
	TTTCTTAAGGTGGAGAATAA+TGG	+	chr5.1:56728427-56728446	MS.gene67269:CDS	30.0%
!	AGCAATTAGAGGGATTTTTA+TGG	+	chr5.1:56727958-56727977	MS.gene67269:CDS	30.0%
!	AGCTGCAGATTTTTCTTTTA+TGG	+	chr5.1:56727730-56727749	MS.gene67269:CDS	30.0%
!	CAGCCATGTTGTTATTATTA+TGG	-	chr5.1:56726896-56726915	None:intergenic	30.0%
!	TGCAGATTTTTCTTTTATGG+AGG	+	chr5.1:56727733-56727752	MS.gene67269:CDS	30.0%
	AAAAGAAAAATCTGCAGCTG+AGG	-	chr5.1:56727729-56727748	None:intergenic	35.0%
	AAAGCCGAAGATAGTGAATT+AGG	+	chr5.1:56728307-56728326	MS.gene67269:CDS	35.0%
	AACATTGCGTGACCTAATTT+CGG	+	chr5.1:56727856-56727875	MS.gene67269:CDS	35.0%
	AAGATGTTCACAAAATGCAG+TGG	+	chr5.1:56727594-56727613	MS.gene67269:CDS	35.0%
	AATTCACCGAATCGTTATTC+CGG	-	chr5.1:56727313-56727332	None:intergenic	35.0%
	ACAACTCAAACTCCGAAATT+AGG	-	chr5.1:56727871-56727890	None:intergenic	35.0%
	ATCAGTATAGTGTGTCTTTG+AGG	+	chr5.1:56727900-56727919	MS.gene67269:CDS	35.0%
	ATGAAACGTAGTGAGAGATT+CGG	-	chr5.1:56727061-56727080	None:intergenic	35.0%
	GAAGCTGAAATCATTCAACA+CGG	+	chr5.1:56728489-56728508	MS.gene67269:CDS	35.0%
	TACTATATGGAAGAGATTGG+GGG	+	chr5.1:56727934-56727953	MS.gene67269:CDS	35.0%
	TCCTCTTCTTCAGAATCAAA+CGG	-	chr5.1:56727338-56727357	None:intergenic	35.0%
	TGCTCCTAATTCACTATCTT+CGG	-	chr5.1:56728314-56728333	None:intergenic	35.0%
	TGGATCAATGCAACATAATG+GGG	+	chr5.1:56728447-56728466	MS.gene67269:CDS	35.0%
!	AGAATTTTAAACAACCGCTC+CGG	-	chr5.1:56727824-56727843	None:intergenic	35.0%
!	AGTTGGAGAATTTGATTCGA+AGG	+	chr5.1:56727981-56728000	MS.gene67269:CDS	35.0%
!	GGTGTTTGATGATTTATGCA+GGG	+	chr5.1:56728510-56728529	MS.gene67269:CDS	35.0%
!	GTTGGAGAATTTGATTCGAA+GGG	+	chr5.1:56727982-56728001	MS.gene67269:CDS	35.0%
!	TAAGGTGTTTGGGTTTCTTA+AGG	+	chr5.1:56728414-56728433	MS.gene67269:CDS	35.0%
!	TAGAGGGATTTTTATGGAGT+TGG	+	chr5.1:56727964-56727983	MS.gene67269:CDS	35.0%
!	TCTTTTATGGAGGTTTGTAG+AGG	+	chr5.1:56727743-56727762	MS.gene67269:CDS	35.0%
!!!	AGTGAATTAGGAGCACTTTT+AGG	+	chr5.1:56728319-56728338	MS.gene67269:CDS	35.0%
	AAGAAAACGTAGCACAAAGC+AGG	-	chr5.1:56728259-56728278	None:intergenic	40.0%
	AGAAAACGTAGCACAAAGCA+GGG	-	chr5.1:56728258-56728277	None:intergenic	40.0%
	AGAAGAGAAATTACTCGCTG+TGG	+	chr5.1:56726845-56726864	MS.gene67269:CDS	40.0%
	AGACTATGGACATCCATTGA+AGG	+	chr5.1:56728117-56728136	MS.gene67269:CDS	40.0%
	AGATGAGTTTCGTTCTCTCA+TGG	+	chr5.1:56727250-56727269	MS.gene67269:CDS	40.0%
	AGCTTCTTTGGTACTGCATA+TGG	-	chr5.1:56728387-56728406	None:intergenic	40.0%
	AGGAGAGTTTGTCGAGATTA+GGG	+	chr5.1:56727552-56727571	MS.gene67269:CDS	40.0%
	CCTTAATCCATGAGCTTCTT+TGG	-	chr5.1:56728399-56728418	None:intergenic	40.0%
	GCATTCATCTTCGTCTTTGT+CGG	+	chr5.1:56728165-56728184	MS.gene67269:CDS	40.0%
	GGAGCTATTAGAGAGCAATT+TGG	+	chr5.1:56728207-56728226	MS.gene67269:CDS	40.0%
	GTTGTTGAATTTCGCAGATG+CGG	+	chr5.1:56727778-56727797	MS.gene67269:CDS	40.0%
	TATGCAGGGTTCAATCTACA+TGG	+	chr5.1:56728524-56728543	MS.gene67269:CDS	40.0%
	TGCATCCTATCATCCATCTT+AGG	-	chr5.1:56728148-56728167	None:intergenic	40.0%
	TTCATCACGTAACGCGTAAT+CGG	-	chr5.1:56728046-56728065	None:intergenic	40.0%
	TTTCGATGGAAGATTCTCGA+AGG	+	chr5.1:56726941-56726960	MS.gene67269:CDS	40.0%
!	ACCGTTTGATTCTGAAGAAG+AGG	+	chr5.1:56727334-56727353	MS.gene67269:CDS	40.0%
!	AGCAGGGTCCTTATAGATTT+TGG	-	chr5.1:56728242-56728261	None:intergenic	40.0%
!	AGCTCATGGATTAAGGTGTT+TGG	+	chr5.1:56728403-56728422	MS.gene67269:CDS	40.0%
!	CCAAAGAAGCTCATGGATTA+AGG	+	chr5.1:56728396-56728415	MS.gene67269:CDS	40.0%
!	CGGTGTTTGATGATTTATGC+AGG	+	chr5.1:56728509-56728528	MS.gene67269:CDS	40.0%
!	GAATTTGATTCGAAGGGATC+CGG	+	chr5.1:56727988-56728007	MS.gene67269:CDS	40.0%
!	GAGCAAGTCTTTGAAGACTA+TGG	+	chr5.1:56728103-56728122	MS.gene67269:CDS	40.0%
!	GCTCATGGATTAAGGTGTTT+GGG	+	chr5.1:56728404-56728423	MS.gene67269:CDS	40.0%
!	GGAGCGGTTGTTTAAAATTC+TGG	+	chr5.1:56727823-56727842	MS.gene67269:CDS	40.0%
!	TGATTCTGAAGAAGAGGATG+AGG	+	chr5.1:56727340-56727359	MS.gene67269:CDS	40.0%
!!	GAGCACTTTTAGGAGATGAT+TGG	+	chr5.1:56728329-56728348	MS.gene67269:CDS	40.0%
!!	GGTGCAAATGGATTGGATTA+TGG	+	chr5.1:56728186-56728205	MS.gene67269:CDS	40.0%
!!!	TTATGCGATCGCGTGTTTTT+TGG	+	chr5.1:56727698-56727717	MS.gene67269:CDS	40.0%
	AAACGTAGTGAGAGATTCGG+CGG	-	chr5.1:56727058-56727077	None:intergenic	45.0%
	ACACTCTTTCCCAAATCCAC+CGG	-	chr5.1:56727495-56727514	None:intergenic	45.0%
	ACCGAATCGTTATTCCGGTA+CGG	-	chr5.1:56727308-56727327	None:intergenic	45.0%
	AGATCGTTAATCGTCGCTGA+CGG	-	chr5.1:56727446-56727465	None:intergenic	45.0%
	AGATTGGGGGAAGCAATTAG+AGG	+	chr5.1:56727947-56727966	MS.gene67269:CDS	45.0%
	CAGAGATCCAAGAGCTTTCA+CGG	+	chr5.1:56727004-56727023	MS.gene67269:CDS	45.0%
	CGTCACATAGCAAAAACGCT+AGG	+	chr5.1:56726870-56726889	MS.gene67269:CDS	45.0%
	CTTTGTCGGTGCAAATGGAT+TGG	+	chr5.1:56728179-56728198	MS.gene67269:CDS	45.0%
	GAAGAGGATGAGGAAATCGA+CGG	+	chr5.1:56727350-56727369	MS.gene67269:CDS	45.0%
	GAGGAGAGTTTGTCGAGATT+AGG	+	chr5.1:56727551-56727570	MS.gene67269:CDS	45.0%
	GATTGGGGGAAGCAATTAGA+GGG	+	chr5.1:56727948-56727967	MS.gene67269:CDS	45.0%
	GGAAGCTACTAAGTCGTCAA+CGG	-	chr5.1:56727135-56727154	None:intergenic	45.0%
	TAGTTGCCGGAGAGAATTCT+TGG	+	chr5.1:56727529-56727548	MS.gene67269:CDS	45.0%
	TCTCCTCCAAGAATTCTCTC+CGG	-	chr5.1:56727538-56727557	None:intergenic	45.0%
	TGCAGTACCAAAGAAGCTCA+TGG	+	chr5.1:56728389-56728408	MS.gene67269:CDS	45.0%
	TGGTGAAGAGGATCTGATTC+CGG	+	chr5.1:56727376-56727395	MS.gene67269:CDS	45.0%
	TGTTCACAAAATGCAGTGGC+AGG	+	chr5.1:56727598-56727617	MS.gene67269:CDS	45.0%
	TTCGTCTTTGTCGGTGCAAA+TGG	+	chr5.1:56728174-56728193	MS.gene67269:CDS	45.0%
	TTTCGTTCTCTCATGGAACG+CGG	+	chr5.1:56727257-56727276	MS.gene67269:CDS	45.0%
!	GATTTGTTTCAGGGAAGCAG+CGG	+	chr5.1:56728715-56728734	MS.gene67269:CDS	45.0%
!	GGTGTTTGGGTTTCTTAAGG+TGG	+	chr5.1:56728417-56728436	MS.gene67269:CDS	45.0%
!	TCTTTCTGAGAAAGTCGCTG+CGG	+	chr5.1:56726968-56726987	MS.gene67269:CDS	45.0%
	ATCGCGAAACGAATGGTTGC+CGG	+	chr5.1:56727473-56727492	MS.gene67269:CDS	50.0%
	ATTACCTTCGTGCTGCGTGT+AGG	+	chr5.1:56728065-56728084	MS.gene67269:CDS	50.0%
	CGCATGTGTACAGTAGTTGC+CGG	+	chr5.1:56727516-56727535	MS.gene67269:CDS	50.0%
	GAATCGTTATTCCGGTACGG+CGG	-	chr5.1:56727305-56727324	None:intergenic	50.0%
	GCCGTACCGGAATAACGATT+CGG	+	chr5.1:56727304-56727323	MS.gene67269:CDS	50.0%
	TAGTAGCTTCCGTTGCAGAG+TGG	+	chr5.1:56727144-56727163	MS.gene67269:CDS	50.0%
	TCACGAGATCGCGAAACGAA+TGG	+	chr5.1:56727466-56727485	MS.gene67269:CDS	50.0%
	TCAGCAGAGTCTGCCCAAAT+TGG	-	chr5.1:56727092-56727111	None:intergenic	50.0%
	TCCAAAGCCGTGAAAGCTCT+TGG	-	chr5.1:56727014-56727033	None:intergenic	50.0%
	TCCAAGAGCTTTCACGGCTT+TGG	+	chr5.1:56727010-56727029	MS.gene67269:CDS	50.0%
	TCGCATAATCGTCGCTCACT+CGG	-	chr5.1:56727686-56727705	None:intergenic	50.0%
	TGGATTTATCACCGGTGAGG+TGG	-	chr5.1:56727172-56727191	None:intergenic	50.0%
	TTAGCTTCGCTTTCGGCTAC+CGG	-	chr5.1:56728746-56728765	None:intergenic	50.0%
	TTGCCGGAGAGAATTCTTGG+AGG	+	chr5.1:56727532-56727551	MS.gene67269:CDS	50.0%
!	CGATGGATTTATCACCGGTG+AGG	-	chr5.1:56727175-56727194	None:intergenic	50.0%
!	CGTGGCGATGGATTTATCAC+CGG	-	chr5.1:56727180-56727199	None:intergenic	50.0%
!	GAATGGTTGCCGGTGGATTT+GGG	+	chr5.1:56727483-56727502	MS.gene67269:CDS	50.0%
	ATCGGATGTAGTCCACCACC+CGG	-	chr5.1:56728028-56728047	None:intergenic	55.0%
	ATGCGGTTGCGATTGGAAGC+CGG	+	chr5.1:56727795-56727814	MS.gene67269:CDS	55.0%
	GAGGATGAGGAAATCGACGG+TGG	+	chr5.1:56727353-56727372	MS.gene67269:CDS	55.0%
	GAGGTGGTTCCACTCTGCAA+CGG	-	chr5.1:56727156-56727175	None:intergenic	55.0%
	GATGAGGAAATCGACGGTGG+TGG	+	chr5.1:56727356-56727375	MS.gene67269:CDS	55.0%
	GATTCGAAGGGATCCGGCTA+AGG	+	chr5.1:56727994-56728013	MS.gene67269:CDS	55.0%
	TATTCCGGTACGGCGGAGTT+AGG	-	chr5.1:56727298-56727317	None:intergenic	55.0%
	TCTGATTCCGGTTGCGATGC+CGG	+	chr5.1:56727388-56727407	MS.gene67269:CDS	55.0%
	TTCGCAGATGCGGTTGCGAT+TGG	+	chr5.1:56727788-56727807	MS.gene67269:CDS	55.0%
	TTGACCTAACTCCGCCGTAC+CGG	+	chr5.1:56727291-56727310	MS.gene67269:CDS	55.0%
!	AACGGCGTCGAGAAAAGCAG+CGG	-	chr5.1:56727117-56727136	None:intergenic	55.0%
!	AATCGACGGTGGTGGTGAAG+AGG	+	chr5.1:56727364-56727383	MS.gene67269:CDS	55.0%
!	CGAATGGTTGCCGGTGGATT+TGG	+	chr5.1:56727482-56727501	MS.gene67269:CDS	55.0%
	AGTGAGAGATTCGGCGGTCG+AGG	-	chr5.1:56727052-56727071	None:intergenic	60.0%
	CAGGGAAGCAGCGGATCAAC+CGG	+	chr5.1:56728724-56728743	MS.gene67269:CDS	60.0%
	CGTTCTCTCATGGAACGCGG+TGG	+	chr5.1:56727260-56727279	MS.gene67269:CDS	60.0%
	GCAGAGTGGAACCACCTCAC+CGG	+	chr5.1:56727158-56727177	MS.gene67269:CDS	60.0%
	GCGAAACGAATGGTTGCCGG+TGG	+	chr5.1:56727476-56727495	MS.gene67269:CDS	60.0%
	GTGTAGGTCGCGGCAGACTT+TGG	+	chr5.1:56728081-56728100	MS.gene67269:CDS	60.0%
	TAAACAACCGCTCCGGCGAC+CGG	-	chr5.1:56727817-56727836	None:intergenic	60.0%
	TCCGGTTGCGATGCCGGTAA+CGG	+	chr5.1:56727394-56727413	MS.gene67269:CDS	60.0%
	TCCGTTACCGGCATCGCAAC+CGG	-	chr5.1:56727398-56727417	None:intergenic	60.0%
	TCGAAGGGATCCGGCTAAGG+CGG	+	chr5.1:56727997-56728016	MS.gene67269:CDS	60.0%
	TGAGAAAGTCGCTGCGGTCG+AGG	+	chr5.1:56726974-56726993	MS.gene67269:CDS	60.0%
	TTCGTGCTGCGTGTAGGTCG+CGG	+	chr5.1:56728071-56728090	MS.gene67269:CDS	60.0%
	CACGTGTCTTGCACGCGCTG+AGG	+	chr5.1:56727196-56727215	MS.gene67269:CDS	65.0%
	CGAAGGGATCCGGCTAAGGC+GGG	+	chr5.1:56727998-56728017	MS.gene67269:CDS	65.0%
	CTCAGCGCGTGCAAGACACG+TGG	-	chr5.1:56727198-56727217	None:intergenic	65.0%
	GCGACCTACACGCAGCACGA+AGG	-	chr5.1:56728072-56728091	None:intergenic	65.0%
	GCGTGCAAGACACGTGGCGA+TGG	-	chr5.1:56727192-56727211	None:intergenic	65.0%
	TCCGGCTAAGGCGGGTGTTC+CGG	+	chr5.1:56728006-56728025	MS.gene67269:CDS	65.0%
	TGCGATTGGAAGCCGGTCGC+CGG	+	chr5.1:56727802-56727821	MS.gene67269:CDS	65.0%
	AAGGCGGGTGTTCCGGGTGG+TGG	+	chr5.1:56728013-56728032	MS.gene67269:CDS	70.0%
	CCCGGAACACCCGCCTTAGC+CGG	-	chr5.1:56728010-56728029	None:intergenic	70.0%
	CCGGCTAAGGCGGGTGTTCC+GGG	+	chr5.1:56728007-56728026	MS.gene67269:CDS	70.0%
	GCTAAGGCGGGTGTTCCGGG+TGG	+	chr5.1:56728010-56728029	MS.gene67269:CDS	70.0%
	TTGGAAGCCGGTCGCCGGAG+CGG	+	chr5.1:56727807-56727826	MS.gene67269:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	56726831	56728765	56726831	ID=MS.gene67269
chr5.1	mRNA	56726831	56728765	56726831	ID=MS.gene67269.t1;Parent=MS.gene67269
chr5.1	exon	56726831	56728765	56726831	ID=MS.gene67269.t1.exon1;Parent=MS.gene67269.t1
chr5.1	CDS	56726831	56728765	56726831	ID=cds.MS.gene67269.t1;Parent=MS.gene67269.t1

Gene Sequence

>MS.gene67269

ATGTCTGAAAACGGAGAAGAGAAATTACTCGCTGTGGCACGTCACATAGCAAAAACGCTAGGCCATAATAATAACAACATGGCTGATGATATTCTTCAAATCTTCTCAAATTTCGATGGAAGATTCTCGAAGGAGAATCTTTCTGAGAAAGTCGCTGCGGTCGAGGTTACTGACAGAGATCCAAGAGCTTTCACGGCTTTGGATCACTGTCTTAAAAACCTCGACCGCCGAATCTCTCACTACGTTTCATCAGATCATCCAATTTGGGCAGACTCTGCTGATGCCGCTGCTTTTCTCGACGCCGTTGACGACTTAGTAGCTTCCGTTGCAGAGTGGAACCACCTCACCGGTGATAAATCCATCGCCACGTGTCTTGCACGCGCTGAGGATATGCTTCAACATGCTATGTTTCGTCTTGAAGATGAGTTTCGTTCTCTCATGGAACGCGGTGGTGAGTCGTTTGACCTAACTCCGCCGTACCGGAATAACGATTCGGTGAATTTACCGTTTGATTCTGAAGAAGAGGATGAGGAAATCGACGGTGGTGGTGAAGAGGATCTGATTCCGGTTGCGATGCCGGTAACGGATTATGAAATTGTGATTGATGCGTTACCGTCAGCGACGATTAACGATCTTCACGAGATCGCGAAACGAATGGTTGCCGGTGGATTTGGGAAAGAGTGTTCGCATGTGTACAGTAGTTGCCGGAGAGAATTCTTGGAGGAGAGTTTGTCGAGATTAGGGTTACAGAAGCTGAGTATTGAAGATGTTCACAAAATGCAGTGGCAGGATATTGAAGATGAAATTGAAAGATGGATTAAAGGTTTCAATGTTGCTTTGAAAATACTTTTTCCGAGTGAGCGACGATTATGCGATCGCGTGTTTTTTGGATTCTCCTCAGCTGCAGATTTTTCTTTTATGGAGGTTTGTAGAGGTTCTACAGTTCAGTTGTTGAATTTCGCAGATGCGGTTGCGATTGGAAGCCGGTCGCCGGAGCGGTTGTTTAAAATTCTGGATGTGTTTGAAACATTGCGTGACCTAATTTCGGAGTTTGAGTTGTTGTTCTGTGATCAGTATAGTGTGTCTTTGAGGAATGAAGCAATTACTATATGGAAGAGATTGGGGGAAGCAATTAGAGGGATTTTTATGGAGTTGGAGAATTTGATTCGAAGGGATCCGGCTAAGGCGGGTGTTCCGGGTGGTGGACTACATCCGATTACGCGTTACGTGATGAATTACCTTCGTGCTGCGTGTAGGTCGCGGCAGACTTTGGAGCAAGTCTTTGAAGACTATGGACATCCATTGAAGGATTATCCTAAGATGGATGATAGGATGCATTCATCTTCGTCTTTGTCGGTGCAAATGGATTGGATTATGGAGCTATTAGAGAGCAATTTGGAAGCAAAATCCAAAATCTATAAGGACCCTGCTTTGTGCTACGTTTTCTTGATGAATAATTGCAGGTACATTGTTCAAAAAGCCGAAGATAGTGAATTAGGAGCACTTTTAGGAGATGATTGGATCAAAAAACACACTGCAAAAATTCGGCAGTACCATATGCAGTACCAAAGAAGCTCATGGATTAAGGTGTTTGGGTTTCTTAAGGTGGAGAATAATGGATCAATGCAACATAATGGGGTAGCTAAGTCAATGAAAGAGAAGCTGAAATCATTCAACACGGTGTTTGATGATTTATGCAGGGTTCAATCTACATGGTTTATCTTTGATGAACAACTTAAGGAAGAAATAAGAATTTCGATTGAGAAACTCTTGTTACCAGCTTATGCAAATTTCATTGTGAGGTTTCAAAATGTTGCTGAAGTTGGTAAACATGCTGATAAATATATTAAGTATGGAACAGAGGACATTGAAGCAAAACTCAATGATTTGTTTCAGGGAAGCAGCGGATCAACCGGTAGCCGAAAGCGAAGCTAA

Protein sequence

>MS.gene67269.t1

MSENGEEKLLAVARHIAKTLGHNNNNMADDILQIFSNFDGRFSKENLSEKVAAVEVTDRDPRAFTALDHCLKNLDRRISHYVSSDHPIWADSADAAAFLDAVDDLVASVAEWNHLTGDKSIATCLARAEDMLQHAMFRLEDEFRSLMERGGESFDLTPPYRNNDSVNLPFDSEEEDEEIDGGGEEDLIPVAMPVTDYEIVIDALPSATINDLHEIAKRMVAGGFGKECSHVYSSCRREFLEESLSRLGLQKLSIEDVHKMQWQDIEDEIERWIKGFNVALKILFPSERRLCDRVFFGFSSAADFSFMEVCRGSTVQLLNFADAVAIGSRSPERLFKILDVFETLRDLISEFELLFCDQYSVSLRNEAITIWKRLGEAIRGIFMELENLIRRDPAKAGVPGGGLHPITRYVMNYLRAACRSRQTLEQVFEDYGHPLKDYPKMDDRMHSSSSLSVQMDWIMELLESNLEAKSKIYKDPALCYVFLMNNCRYIVQKAEDSELGALLGDDWIKKHTAKIRQYHMQYQRSSWIKVFGFLKVENNGSMQHNGVAKSMKEKLKSFNTVFDDLCRVQSTWFIFDEQLKEEIRISIEKLLLPAYANFIVRFQNVAEVGKHADKYIKYGTEDIEAKLNDLFQGSSGSTGSRKRS