Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68115.t1 | XP_024635233.1 | 96.2 | 442 | 6 | 1 | 1 | 431 | 1 | 442 | 5.20E-244 | 853.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68115.t1 | Q9XGM8 | 69.8 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 8.9e-184 | 644.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68115.t1 | A0A1S2Y7Q7 | 88.0 | 442 | 42 | 1 | 1 | 431 | 1 | 442 | 9.6e-224 | 785.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene68115 | 0.821963 | 3.05E-53 | -1.69E-46 |
| MS.gene049647 | MS.gene68115 | 0.801556 | 8.51E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene68115 | 0.830891 | 2.27E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene68115 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene68115 | 0.816571 | 5.16E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene68115 | 0.809646 | 1.71E-50 | -1.69E-46 |
| MS.gene050439 | MS.gene68115 | 0.822039 | 2.93E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene68115 | 0.848136 | 7.43E-60 | -1.69E-46 |
| MS.gene050721 | MS.gene68115 | 0.847443 | 1.15E-59 | -1.69E-46 |
| MS.gene050724 | MS.gene68115 | 0.824572 | 7.51E-54 | -1.69E-46 |
| MS.gene050762 | MS.gene68115 | 0.820993 | 5.11E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene68115 | 0.821215 | 4.55E-53 | -1.69E-46 |
| MS.gene050780 | MS.gene68115 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene051210 | MS.gene68115 | 0.825072 | 5.72E-54 | -1.69E-46 |
| MS.gene052665 | MS.gene68115 | 0.809703 | 1.66E-50 | -1.69E-46 |
| MS.gene053811 | MS.gene68115 | 0.818131 | 2.30E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene68115 | 0.810697 | 1.01E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene68115 | 0.820827 | 5.58E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene68115 | 0.819801 | 9.60E-53 | -1.69E-46 |
| MS.gene05468 | MS.gene68115 | 0.808456 | 3.07E-50 | -1.69E-46 |
| MS.gene056432 | MS.gene68115 | 0.810901 | 9.15E-51 | -1.69E-46 |
| MS.gene056552 | MS.gene68115 | 0.814595 | 1.42E-51 | -1.69E-46 |
| MS.gene056638 | MS.gene68115 | 0.803543 | 3.32E-49 | -1.69E-46 |
| MS.gene057360 | MS.gene68115 | 0.821296 | 4.35E-53 | -1.69E-46 |
| MS.gene058182 | MS.gene68115 | 0.820718 | 5.92E-53 | -1.69E-46 |
| MS.gene058638 | MS.gene68115 | 0.801772 | 7.69E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene68115 | 0.812673 | 3.77E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene68115 | 0.801353 | 9.37E-49 | -1.69E-46 |
| MS.gene059713 | MS.gene68115 | 0.809746 | 1.62E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene68115 | 0.817882 | 2.62E-52 | -1.69E-46 |
| MS.gene060637 | MS.gene68115 | 0.852874 | 3.47E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68115.t1 | MTR_3g118520 | 97.544 | 285 | 7 | 0 | 1 | 285 | 1 | 285 | 0.0 | 578 |
| MS.gene68115.t1 | MTR_3g118525 | 98.438 | 128 | 2 | 0 | 304 | 431 | 15 | 142 | 2.39e-86 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68115.t1 | AT4G38240 | 70.930 | 430 | 125 | 0 | 1 | 430 | 1 | 430 | 0.0 | 667 |
| MS.gene68115.t1 | AT4G38240 | 69.841 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 0.0 | 665 |
| MS.gene68115.t1 | AT4G38240 | 69.841 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 0.0 | 665 |
Find 75 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACATTGCTCAACAATT+TGG | 0.214206 | 3.4:+100331629 | MS.gene68115:CDS |
| GGATATGCCTTCGGCCATTT+TGG | 0.228883 | 3.4:-100330586 | None:intergenic |
| TGAAGTAGCTTCAAAGATTC+TGG | 0.231515 | 3.4:-100331893 | None:intergenic |
| TATCGCTCGGACTTCTTTCC+TGG | 0.251249 | 3.4:+100330520 | MS.gene68115:CDS |
| TTTGGGAAATGGCGAAATTT+TGG | 0.251374 | 3.4:+100323946 | None:intergenic |
| TAAAGGAAATCTTGAAGAAA+TGG | 0.304704 | 3.4:-100324821 | None:intergenic |
| TATCCATTCTAGGTCCATTA+TGG | 0.306360 | 3.4:+100329275 | MS.gene68115:intron |
| CCTGGCCTTTCAGTTTGAAC+TGG | 0.312664 | 3.4:-100328812 | None:intergenic |
| TCTTCTCTTTGTTGCAGCCT+TGG | 0.315376 | 3.4:+100323983 | MS.gene68115:CDS |
| CTTGAACAAGAGACTTGATT+TGG | 0.319300 | 3.4:-100324387 | None:intergenic |
| TGGACTTCATTCAGCTTGAT+TGG | 0.364035 | 3.4:-100331271 | None:intergenic |
| TTATATGTTCTGCATACTTC+GGG | 0.365657 | 3.4:-100330909 | None:intergenic |
| GGGATGAACTATCACCAAAA+TGG | 0.369625 | 3.4:+100330572 | MS.gene68115:CDS |
| GTTTCGTCATGGAATGATAA+TGG | 0.381929 | 3.4:+100329300 | MS.gene68115:CDS |
| AATGGACTTGAGCTATTTGT+TGG | 0.383291 | 3.4:+100331393 | MS.gene68115:CDS |
| CCATGACGAAACAGCCATAA+TGG | 0.392943 | 3.4:-100329289 | None:intergenic |
| AATACCTGCGATACAAATAA+AGG | 0.398527 | 3.4:-100324838 | None:intergenic |
| CTTTGATTACTTTGAAGCTA+TGG | 0.412034 | 3.4:+100329135 | MS.gene68115:CDS |
| TCAAAGTAATCAAAGAAATC+AGG | 0.439796 | 3.4:-100329127 | None:intergenic |
| AAAGGAAATCTTGAAGAAAT+GGG | 0.442893 | 3.4:-100324820 | None:intergenic |
| ACGAAGCGTATATTCCTTGT+TGG | 0.443306 | 3.4:+100331869 | MS.gene68115:CDS |
| GTGCCTAGGACAGCTTATAA+AGG | 0.447968 | 3.4:+100331821 | MS.gene68115:CDS |
| GTTATATGTTCTGCATACTT+CGG | 0.455588 | 3.4:-100330910 | None:intergenic |
| GGTCATTACAAGTGGGCTCT+TGG | 0.466698 | 3.4:+100328946 | MS.gene68115:intron |
| GAAGTAGCTTCAAAGATTCT+GGG | 0.477829 | 3.4:-100331892 | None:intergenic |
| TCGGACTTCTTTCCTGGACT+AGG | 0.478118 | 3.4:+100330526 | MS.gene68115:CDS |
| GCGAAGTCTGCTCCAGAGAC+AGG | 0.486412 | 3.4:-100331545 | None:intergenic |
| AAATTCAGACGTCAAAAGTA+AGG | 0.488269 | 3.4:+100324998 | MS.gene68115:CDS |
| AGTGGCAGCTGTTGTCATCA+TGG | 0.497854 | 3.4:+100324656 | MS.gene68115:CDS |
| TAGTAAGCAATTAACTCTCC+TGG | 0.506538 | 3.4:-100328830 | None:intergenic |
| ATTTCCTTTATTTGTATCGC+AGG | 0.508351 | 3.4:+100324834 | MS.gene68115:CDS |
| TGCAGACGAACTTTATCGCT+CGG | 0.520518 | 3.4:+100330507 | MS.gene68115:intron |
| AACAAGGAATATACGCTTCG+TGG | 0.520785 | 3.4:-100331867 | None:intergenic |
| CTTGGTGTTCATCTACATCC+AGG | 0.522745 | 3.4:+100324001 | MS.gene68115:CDS |
| GGCTACATTCTTGCTCTCGA+CGG | 0.523455 | 3.4:-100324366 | None:intergenic |
| GTATGCAGAACATATAACTT+TGG | 0.523714 | 3.4:+100330915 | MS.gene68115:CDS |
| TGGTATATTTCAAGAGTGGA+AGG | 0.526230 | 3.4:+100331649 | MS.gene68115:CDS |
| GCCAGCATCCATCCTAGTCC+AGG | 0.526290 | 3.4:-100330538 | None:intergenic |
| CAGAATTGATAGTCCTCTCC+AGG | 0.527635 | 3.4:-100324698 | None:intergenic |
| ACTTCTTTCCTGGACTAGGA+TGG | 0.531658 | 3.4:+100330530 | MS.gene68115:CDS |
| TCCTGGACTAGGATGGATGC+TGG | 0.534970 | 3.4:+100330537 | MS.gene68115:CDS |
| TTGAGCCAGTTCAAACTGAA+AGG | 0.535555 | 3.4:+100328807 | MS.gene68115:CDS |
| CCATTATGGCTGTTTCGTCA+TGG | 0.538200 | 3.4:+100329289 | MS.gene68115:CDS |
| GAAGGTCGACTGGAAGTCAA+TGG | 0.538850 | 3.4:+100331375 | MS.gene68115:intron |
| AATCAAGCTGAATGAAGTCC+AGG | 0.541494 | 3.4:+100331273 | MS.gene68115:CDS |
| TTCTCGACTTACAACAAGAA+AGG | 0.541791 | 3.4:+100324341 | MS.gene68115:CDS |
| ACTCCTTTATAAGCTGTCCT+AGG | 0.545192 | 3.4:-100331824 | None:intergenic |
| ATGTAATCGTGCTGATTACC+TGG | 0.547620 | 3.4:+100324680 | MS.gene68115:CDS |
| GGATGCTGGCTAGATCTACA+TGG | 0.553110 | 3.4:+100330551 | MS.gene68115:CDS |
| CCAGTTCAAACTGAAAGGCC+AGG | 0.554999 | 3.4:+100328812 | MS.gene68115:CDS |
| GGACTTGAGCTATTTGTTGG+AGG | 0.555050 | 3.4:+100331396 | MS.gene68115:CDS |
| TGGAGCAGACTTCGCTCAGA+AGG | 0.556130 | 3.4:+100331553 | MS.gene68115:CDS |
| CGATGAGCTATCTCATATGC+AGG | 0.562275 | 3.4:+100325031 | MS.gene68115:CDS |
| ATGATGACAACAGCTGCCAC+TGG | 0.562931 | 3.4:-100324654 | None:intergenic |
| TGGATTGAATGAAGGTCGAC+TGG | 0.576838 | 3.4:+100331365 | MS.gene68115:intron |
| ACTTCCAGGATGGTGTGCCT+AGG | 0.576852 | 3.4:+100331807 | MS.gene68115:intron |
| AAGGCTGATTGATAAAGTCC+AGG | 0.584981 | 3.4:+100324535 | MS.gene68115:CDS |
| TTATCCAACCTCAGATGACA+TGG | 0.598293 | 3.4:+100329096 | MS.gene68115:intron |
| AATTTGGTATATTTCAAGAG+TGG | 0.599428 | 3.4:+100331645 | MS.gene68115:CDS |
| TATAAAGTATAAAGATCAGT+GGG | 0.600564 | 3.4:+100331601 | MS.gene68115:CDS |
| ATGTTCTGCATACTTCGGGT+CGG | 0.615835 | 3.4:-100330905 | None:intergenic |
| ACACATACTTATCCTTGTCA+AGG | 0.618408 | 3.4:-100329165 | None:intergenic |
| AACAGAGTCATCATTCTCGA+AGG | 0.620252 | 3.4:+100328994 | MS.gene68115:CDS |
| TTTCAGGAAAAGATGTACAA+AGG | 0.627774 | 3.4:+100324516 | MS.gene68115:intron |
| CAGAAGGCACGCAATATAGA+CGG | 0.629864 | 3.4:+100331569 | MS.gene68115:CDS |
| TCAAAGATTCTGGGCCAACA+AGG | 0.632429 | 3.4:-100331883 | None:intergenic |
| ATCGTGCTGATTACCTGGAG+AGG | 0.643089 | 3.4:+100324685 | MS.gene68115:CDS |
| GATCAGATTAGCTTGCAACA+AGG | 0.650241 | 3.4:+100324313 | MS.gene68115:CDS |
| ACTATCACCAAAATGGCCGA+AGG | 0.673152 | 3.4:+100330579 | MS.gene68115:CDS |
| GTATAAAGTATAAAGATCAG+TGG | 0.683838 | 3.4:+100331600 | MS.gene68115:CDS |
| GATGCTGGCTAGATCTACAT+GGG | 0.685395 | 3.4:+100330552 | MS.gene68115:CDS |
| TATGGCAACTCTCCTTGACA+AGG | 0.700285 | 3.4:+100329153 | MS.gene68115:CDS |
| AGATTAAAAGAGAATCACAA+AGG | 0.714494 | 3.4:+100330870 | MS.gene68115:intron |
| AATTACATACAGCAACAGCG+AGG | 0.779431 | 3.4:-100324160 | None:intergenic |
| CTGGATGTAGATGAACACCA+AGG | 0.780942 | 3.4:-100324000 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 100323954 | 100331925 | 100323954 | ID=MS.gene68115 |
| chr3.4 | mRNA | 100323954 | 100331925 | 100323954 | ID=MS.gene68115.t1;Parent=MS.gene68115 |
| chr3.4 | exon | 100323954 | 100324022 | 100323954 | ID=MS.gene68115.t1.exon1;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100323954 | 100324022 | 100323954 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324122 | 100324172 | 100324122 | ID=MS.gene68115.t1.exon2;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324122 | 100324172 | 100324122 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324268 | 100324418 | 100324268 | ID=MS.gene68115.t1.exon3;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324268 | 100324418 | 100324268 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324522 | 100324556 | 100324522 | ID=MS.gene68115.t1.exon4;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324522 | 100324556 | 100324522 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324651 | 100324727 | 100324651 | ID=MS.gene68115.t1.exon5;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324651 | 100324727 | 100324651 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324810 | 100324855 | 100324810 | ID=MS.gene68115.t1.exon6;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324810 | 100324855 | 100324810 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100324990 | 100325052 | 100324990 | ID=MS.gene68115.t1.exon7;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100324990 | 100325052 | 100324990 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100328797 | 100328863 | 100328797 | ID=MS.gene68115.t1.exon8;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100328797 | 100328863 | 100328797 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100328947 | 100329015 | 100328947 | ID=MS.gene68115.t1.exon9;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100328947 | 100329015 | 100328947 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100329110 | 100329179 | 100329110 | ID=MS.gene68115.t1.exon10;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100329110 | 100329179 | 100329110 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100329287 | 100329348 | 100329287 | ID=MS.gene68115.t1.exon11;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100329287 | 100329348 | 100329287 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100330512 | 100330600 | 100330512 | ID=MS.gene68115.t1.exon12;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100330512 | 100330600 | 100330512 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100330882 | 100330944 | 100330882 | ID=MS.gene68115.t1.exon13;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100330882 | 100330944 | 100330882 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100331232 | 100331294 | 100331232 | ID=MS.gene68115.t1.exon14;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100331232 | 100331294 | 100331232 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100331379 | 100331417 | 100331379 | ID=MS.gene68115.t1.exon15;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100331379 | 100331417 | 100331379 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100331500 | 100331670 | 100331500 | ID=MS.gene68115.t1.exon16;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100331500 | 100331670 | 100331500 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| chr3.4 | exon | 100331815 | 100331925 | 100331815 | ID=MS.gene68115.t1.exon17;Parent=MS.gene68115.t1 |
| chr3.4 | CDS | 100331815 | 100331925 | 100331815 | ID=cds.MS.gene68115.t1;Parent=MS.gene68115.t1 |
| Gene Sequence |
| Protein sequence |
