AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70446


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70446.t1 MTR_3g082970 97.788 226 5 0 1 226 1 226 1.53e-166 458
MS.gene70446.t1 MTR_2g025740 71.875 224 49 2 2 225 23 232 4.18e-110 315
MS.gene70446.t1 MTR_4g133060 70.335 209 62 0 17 225 13 221 4.17e-105 303
MS.gene70446.t1 MTR_4g133060 73.196 194 52 0 32 225 2 195 3.59e-102 294
MS.gene70446.t1 MTR_4g133060 65.366 205 67 1 17 217 13 217 1.12e-90 266
MS.gene70446.t1 MTR_8g097200 67.052 173 57 0 53 225 59 231 8.05e-86 254
MS.gene70446.t1 MTR_3g084940 63.636 176 63 1 52 226 52 227 1.99e-78 235
MS.gene70446.t1 MTR_7g097270 51.337 187 88 3 38 223 33 217 5.89e-63 196
MS.gene70446.t1 MTR_7g097270 49.600 125 60 3 38 161 33 155 4.06e-33 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70446.t1 AT3G07880 77.114 201 45 1 25 225 38 237 3.48e-113 324
MS.gene70446.t1 AT1G62450 64.319 213 73 2 15 225 11 222 1.56e-91 268
MS.gene70446.t1 AT1G12070 59.912 227 84 2 1 225 1 222 1.64e-88 261

Find 56 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGCAATAACATTGTTTC+TGG 0.193009 3.1:+66411694 MS.gene70446:CDS
CATAGGACCTAGCTGTATTT+TGG 0.248019 3.1:-66410959 None:intergenic
CCAGAGGGTGTGACTTCTTC+TGG 0.286287 3.1:-66411926 None:intergenic
AAATGGAAGGAGCAGCTTCT+TGG 0.366946 3.1:+66411169 MS.gene70446:CDS
TGTTTGGAAGACTGCTGTTA+AGG 0.371169 3.1:+66411735 MS.gene70446:CDS
GAGGAGGAAGAAGATGAATA+TGG 0.374764 3.1:+66410934 MS.gene70446:CDS
GTTTGGAAGACTGCTGTTAA+GGG 0.387643 3.1:+66411736 MS.gene70446:CDS
AATGGAAGGAGCAGCTTCTT+GGG 0.389589 3.1:+66411170 MS.gene70446:CDS
TAAAATACACCAATACTGTT+TGG 0.391465 3.1:+66411719 MS.gene70446:CDS
TATAGGACGATGAGAGTCTA+AGG 0.397474 3.1:+66411146 MS.gene70446:intron
GTTCTTCCCATTCCTGAAGA+TGG 0.408171 3.1:+66411604 MS.gene70446:CDS
CCAGAAGAAGTCACACCCTC+TGG 0.416770 3.1:+66411926 MS.gene70446:CDS
AGGCTTGTAAAATTCACTTC+AGG 0.420910 3.1:-66411550 None:intergenic
GATAATGAAAACAAAGAAAC+AGG 0.423638 3.1:+66410826 MS.gene70446:CDS
TCTGGCATCTCATGTGTGTA+AGG 0.427995 3.1:-66411908 None:intergenic
GTGTGTAAGGCTCTGGTTGA+GGG 0.432758 3.1:-66411895 None:intergenic
ATCTCATGTGTGTAAGGCTC+TGG 0.437007 3.1:-66411902 None:intergenic
ACGATGAGAGTCTAAGGAAA+TGG 0.439473 3.1:+66411152 MS.gene70446:CDS
TGAGCAATAACATTGTTTCT+GGG 0.463614 3.1:+66411695 MS.gene70446:CDS
CAAAATACAGCTAGGTCCTA+TGG 0.489028 3.1:+66410960 MS.gene70446:CDS
AGAACACCTTGAGAAAGATA+AGG 0.492651 3.1:+66410993 MS.gene70446:CDS
AGAAGATTCACTCGTATGCC+TGG 0.493330 3.1:-66410899 None:intergenic
TGTGTGTAAGGCTCTGGTTG+AGG 0.496739 3.1:-66411896 None:intergenic
TTGTGGTTTACTCTTAAAGA+AGG 0.514851 3.1:+66411640 MS.gene70446:CDS
CACCCTCTGGGATATTTGCT+AGG 0.518348 3.1:+66411939 MS.gene70446:CDS
GATAGTTCAAAAGAGATGCT+TGG 0.518968 3.1:+66411863 MS.gene70446:CDS
TCCTGAAGATGGAAAACCAC+AGG 0.525613 3.1:+66411615 MS.gene70446:CDS
TGATGACAATAAATGTTACT+TGG 0.527867 3.1:+66412220 MS.gene70446:CDS
AGCCTTTCTATAATCTCTCC+TGG 0.528718 3.1:+66411568 MS.gene70446:CDS
ATGGAAAACCACAGGGATTG+TGG 0.531586 3.1:+66411623 MS.gene70446:CDS
ACCCTCTGGGATATTTGCTA+GGG 0.538296 3.1:+66411940 MS.gene70446:CDS
TCACTCGTATGCCTGGTAAG+AGG 0.546518 3.1:-66410892 None:intergenic
ACAAAACCTTATCTTTCTCA+AGG 0.549448 3.1:-66410999 None:intergenic
AACATCATGAGCCTCTTACC+AGG 0.549984 3.1:+66410881 MS.gene70446:CDS
ACAGGACAAACTAGTGAGGC+TGG 0.550189 3.1:+66410844 MS.gene70446:CDS
AAGCTGAATTTCAGACGGTA+AGG 0.552089 3.1:-66411667 None:intergenic
ACAATATTCTGCAAGATCAA+AGG 0.554037 3.1:+66411964 MS.gene70446:CDS
GTCGATGTCAACAACATTGG+AGG 0.554851 3.1:+66411196 MS.gene70446:CDS
AGCAGTCTTCCAAACAGTAT+TGG 0.558230 3.1:-66411728 None:intergenic
CAGAAGAAGTCACACCCTCT+GGG 0.582116 3.1:+66411927 MS.gene70446:CDS
TACCAGGAGAGATTATAGAA+AGG 0.583890 3.1:-66411570 None:intergenic
TATGGAGCCAAAATACAGCT+AGG 0.601518 3.1:+66410952 MS.gene70446:CDS
TGTTCTTTAATAGTGACCAT+AGG 0.603238 3.1:-66410976 None:intergenic
AGTGTCGATGTCAACAACAT+TGG 0.603658 3.1:+66411193 MS.gene70446:CDS
CCCCTAGCAAATATCCCAGA+GGG 0.617735 3.1:-66411941 None:intergenic
TGAGAGTCTAAGGAAATGGA+AGG 0.619544 3.1:+66411156 MS.gene70446:CDS
TTCTGTCTATGCAACTGAGG+AGG 0.624555 3.1:+66410918 MS.gene70446:CDS
AGAACAATGTCATCTCTACC+AGG 0.626041 3.1:-66411586 None:intergenic
CAACAAAGCTGAATTTCAGA+CGG 0.649490 3.1:-66411672 None:intergenic
CCTGAAGATGGAAAACCACA+GGG 0.658329 3.1:+66411616 MS.gene70446:CDS
CCCTCTGGGATATTTGCTAG+GGG 0.670702 3.1:+66411941 MS.gene70446:CDS
AGAGTAAACCACAATCCCTG+TGG 0.676637 3.1:-66411631 None:intergenic
GGACAAACTAGTGAGGCTGG+TGG 0.680791 3.1:+66410847 MS.gene70446:CDS
ATCTTCTGTCTATGCAACTG+AGG 0.702591 3.1:+66410915 MS.gene70446:CDS
AGAAACAGGACAAACTAGTG+AGG 0.703043 3.1:+66410840 MS.gene70446:CDS
TCCCCTAGCAAATATCCCAG+AGG 0.719574 3.1:-66411942 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGTATAATTTTTTTGT+TGG + chr3.1:66412159-66412178 MS.gene70446:intron 10.0%
!! GTAATTTAAGTAATTTACTT+AGG - chr3.1:66411070-66411089 None:intergenic 15.0%
!! GTTACCAATAAATAATTATG+AGG - chr3.1:66412071-66412090 None:intergenic 20.0%
!! TGTTCCTCATAATTATTTAT+TGG + chr3.1:66412064-66412083 MS.gene70446:intron 20.0%
!! TTGTTACACTTAATATGTAA+CGG - chr3.1:66412095-66412114 None:intergenic 20.0%
!!! TAAAACAAATGTTATTTCAG+TGG + chr3.1:66411841-66411860 MS.gene70446:intron 20.0%
!!! TCAACTATACTTTTGATATT+AGG + chr3.1:66412246-66412265 MS.gene70446:CDS 20.0%
!!! TGAACAATTTTCTTTTACAA+AGG - chr3.1:66411278-66411297 None:intergenic 20.0%
!!! TGTTCATTGTTTATGTTTTT+GGG + chr3.1:66411110-66411129 MS.gene70446:intron 20.0%
!!! TTGTTCATTGTTTATGTTTT+TGG + chr3.1:66411109-66411128 MS.gene70446:intron 20.0%
! GATAATGAAAACAAAGAAAC+AGG + chr3.1:66410826-66410845 MS.gene70446:CDS 25.0%
! GTGTTACAAAATTGGAAATT+TGG - chr3.1:66411796-66411815 None:intergenic 25.0%
! TAAAATACACCAATACTGTT+TGG + chr3.1:66411719-66411738 MS.gene70446:CDS 25.0%
! TGATGACAATAAATGTTACT+TGG + chr3.1:66412220-66412239 MS.gene70446:CDS 25.0%
!! CTATACTTTTGATATTAGGA+AGG + chr3.1:66412250-66412269 MS.gene70446:CDS 25.0%
!! TTTTGATATTAGGAAGGATT+GGG + chr3.1:66412256-66412275 MS.gene70446:CDS 25.0%
!!! AGATAAGGTTTTGTTTCTTT+TGG + chr3.1:66411008-66411027 MS.gene70446:intron 25.0%
ACAAAACCTTATCTTTCTCA+AGG - chr3.1:66411002-66411021 None:intergenic 30.0%
ACAATATTCTGCAAGATCAA+AGG + chr3.1:66411964-66411983 MS.gene70446:CDS 30.0%
AGGGATAAGTGTTACAAAAT+TGG - chr3.1:66411804-66411823 None:intergenic 30.0%
TGTTCTTTAATAGTGACCAT+AGG - chr3.1:66410979-66410998 None:intergenic 30.0%
TTGTGGTTTACTCTTAAAGA+AGG + chr3.1:66411640-66411659 MS.gene70446:CDS 30.0%
! CATTGGAGGTATATAAATCA+CGG + chr3.1:66411210-66411229 MS.gene70446:intron 30.0%
! CTTTTGATATTAGGAAGGAT+TGG + chr3.1:66412255-66412274 MS.gene70446:CDS 30.0%
! TATTTTTCTTGTGCAGTTTC+TGG + chr3.1:66412196-66412215 MS.gene70446:intron 30.0%
! TGAGCAATAACATTGTTTCT+GGG + chr3.1:66411695-66411714 MS.gene70446:CDS 30.0%
AGAACACCTTGAGAAAGATA+AGG + chr3.1:66410993-66411012 MS.gene70446:CDS 35.0%
AGGCTTGTAAAATTCACTTC+AGG - chr3.1:66411553-66411572 None:intergenic 35.0%
CAACAAAGCTGAATTTCAGA+CGG - chr3.1:66411675-66411694 None:intergenic 35.0%
GATAGTTCAAAAGAGATGCT+TGG + chr3.1:66411863-66411882 MS.gene70446:CDS 35.0%
GTGAGCAATAACATTGTTTC+TGG + chr3.1:66411694-66411713 MS.gene70446:CDS 35.0%
TACCAGGAGAGATTATAGAA+AGG - chr3.1:66411573-66411592 None:intergenic 35.0%
TGGGTTGTACGAATGAATAT+AGG + chr3.1:66411129-66411148 MS.gene70446:intron 35.0%
AAGCTGAATTTCAGACGGTA+AGG - chr3.1:66411670-66411689 None:intergenic 40.0%
ACGATGAGAGTCTAAGGAAA+TGG + chr3.1:66411152-66411171 MS.gene70446:CDS 40.0%
AGAAACAGGACAAACTAGTG+AGG + chr3.1:66410840-66410859 MS.gene70446:CDS 40.0%
AGAACAATGTCATCTCTACC+AGG - chr3.1:66411589-66411608 None:intergenic 40.0%
AGCAGTCTTCCAAACAGTAT+TGG - chr3.1:66411731-66411750 None:intergenic 40.0%
AGCCTTTCTATAATCTCTCC+TGG + chr3.1:66411568-66411587 MS.gene70446:CDS 40.0%
ATCTTCTGTCTATGCAACTG+AGG + chr3.1:66410915-66410934 MS.gene70446:CDS 40.0%
CAAAATACAGCTAGGTCCTA+TGG + chr3.1:66410960-66410979 MS.gene70446:CDS 40.0%
GAGGAGGAAGAAGATGAATA+TGG + chr3.1:66410934-66410953 MS.gene70446:CDS 40.0%
GTTTGGAAGACTGCTGTTAA+GGG + chr3.1:66411736-66411755 MS.gene70446:CDS 40.0%
TATAGGACGATGAGAGTCTA+AGG + chr3.1:66411146-66411165 MS.gene70446:intron 40.0%
TATGGAGCCAAAATACAGCT+AGG + chr3.1:66410952-66410971 MS.gene70446:CDS 40.0%
TGAGAGTCTAAGGAAATGGA+AGG + chr3.1:66411156-66411175 MS.gene70446:CDS 40.0%
TGTTTGGAAGACTGCTGTTA+AGG + chr3.1:66411735-66411754 MS.gene70446:CDS 40.0%
! CATAGGACCTAGCTGTATTT+TGG - chr3.1:66410962-66410981 None:intergenic 40.0%
! GGTTTTCCATCTTCAGGAAT+GGG - chr3.1:66411613-66411632 None:intergenic 40.0%
! TGGTTTTCCATCTTCAGGAA+TGG - chr3.1:66411614-66411633 None:intergenic 40.0%
!! AGTGTCGATGTCAACAACAT+TGG + chr3.1:66411193-66411212 MS.gene70446:CDS 40.0%
AAATGGAAGGAGCAGCTTCT+TGG + chr3.1:66411169-66411188 MS.gene70446:CDS 45.0%
AACATCATGAGCCTCTTACC+AGG + chr3.1:66410881-66410900 MS.gene70446:CDS 45.0%
AATGGAAGGAGCAGCTTCTT+GGG + chr3.1:66411170-66411189 MS.gene70446:CDS 45.0%
AGAAGATTCACTCGTATGCC+TGG - chr3.1:66410902-66410921 None:intergenic 45.0%
AGAGTAAACCACAATCCCTG+TGG - chr3.1:66411634-66411653 None:intergenic 45.0%
ATCTCATGTGTGTAAGGCTC+TGG - chr3.1:66411905-66411924 None:intergenic 45.0%
ATGGAAAACCACAGGGATTG+TGG + chr3.1:66411623-66411642 MS.gene70446:CDS 45.0%
CCTGAAGATGGAAAACCACA+GGG + chr3.1:66411616-66411635 MS.gene70446:CDS 45.0%
GTCGATGTCAACAACATTGG+AGG + chr3.1:66411196-66411215 MS.gene70446:CDS 45.0%
GTTCTTCCCATTCCTGAAGA+TGG + chr3.1:66411604-66411623 MS.gene70446:CDS 45.0%
TCCTGAAGATGGAAAACCAC+AGG + chr3.1:66411615-66411634 MS.gene70446:CDS 45.0%
TCTGGCATCTCATGTGTGTA+AGG - chr3.1:66411911-66411930 None:intergenic 45.0%
TTCTGTCTATGCAACTGAGG+AGG + chr3.1:66410918-66410937 MS.gene70446:CDS 45.0%
!! ACAAGTGTGTCGATTGTTGC+AGG + chr3.1:66411431-66411450 MS.gene70446:intron 45.0%
!! ACCCTCTGGGATATTTGCTA+GGG + chr3.1:66411940-66411959 MS.gene70446:CDS 45.0%
!! CAAGTGTGTCGATTGTTGCA+GGG + chr3.1:66411432-66411451 MS.gene70446:intron 45.0%
!! TAATACAATTAAAATTAAAA+GGG - chr3.1:66411823-66411842 None:intergenic 5.0%
!! TTAATACAATTAAAATTAAA+AGG - chr3.1:66411824-66411843 None:intergenic 5.0%
ACAGGACAAACTAGTGAGGC+TGG + chr3.1:66410844-66410863 MS.gene70446:CDS 50.0%
CAGAAGAAGTCACACCCTCT+GGG + chr3.1:66411927-66411946 MS.gene70446:CDS 50.0%
CCCCTAGCAAATATCCCAGA+GGG - chr3.1:66411944-66411963 None:intergenic 50.0%
TCACTCGTATGCCTGGTAAG+AGG - chr3.1:66410895-66410914 None:intergenic 50.0%
TCCCCTAGCAAATATCCCAG+AGG - chr3.1:66411945-66411964 None:intergenic 50.0%
! CCCTGTGGTTTTCCATCTTC+AGG - chr3.1:66411619-66411638 None:intergenic 50.0%
! GTGTGTAAGGCTCTGGTTGA+GGG - chr3.1:66411898-66411917 None:intergenic 50.0%
! TGTGTGTAAGGCTCTGGTTG+AGG - chr3.1:66411899-66411918 None:intergenic 50.0%
!! CACCCTCTGGGATATTTGCT+AGG + chr3.1:66411939-66411958 MS.gene70446:CDS 50.0%
!! CCCTCTGGGATATTTGCTAG+GGG + chr3.1:66411941-66411960 MS.gene70446:CDS 50.0%
CCAGAAGAAGTCACACCCTC+TGG + chr3.1:66411926-66411945 MS.gene70446:CDS 55.0%
GGACAAACTAGTGAGGCTGG+TGG + chr3.1:66410847-66410866 MS.gene70446:CDS 55.0%
! CCAGAGGGTGTGACTTCTTC+TGG - chr3.1:66411929-66411948 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 66410817 66412283 66410817 ID=MS.gene70446
chr3.1 mRNA 66410817 66412283 66410817 ID=MS.gene70446.t1;Parent=MS.gene70446
chr3.1 exon 66410817 66411014 66410817 ID=MS.gene70446.t1.exon1;Parent=MS.gene70446.t1
chr3.1 CDS 66410817 66411014 66410817 ID=cds.MS.gene70446.t1;Parent=MS.gene70446.t1
chr3.1 exon 66411151 66411217 66411151 ID=MS.gene70446.t1.exon2;Parent=MS.gene70446.t1
chr3.1 CDS 66411151 66411217 66411151 ID=cds.MS.gene70446.t1;Parent=MS.gene70446.t1
chr3.1 exon 66411539 66411757 66411539 ID=MS.gene70446.t1.exon3;Parent=MS.gene70446.t1
chr3.1 CDS 66411539 66411757 66411539 ID=cds.MS.gene70446.t1;Parent=MS.gene70446.t1
chr3.1 exon 66411861 66411985 66411861 ID=MS.gene70446.t1.exon4;Parent=MS.gene70446.t1
chr3.1 CDS 66411861 66411985 66411861 ID=cds.MS.gene70446.t1;Parent=MS.gene70446.t1
chr3.1 exon 66412212 66412283 66412212 ID=MS.gene70446.t1.exon5;Parent=MS.gene70446.t1
chr3.1 CDS 66412212 66412283 66412212 ID=cds.MS.gene70446.t1;Parent=MS.gene70446.t1
Gene Sequence

>MS.gene70446

ATGGGGTCTGATAATGAAAACAAAGAAACAGGACAAACTAGTGAGGCTGGTGGAGAAAAAGAAGAACATCATGAGCCTCTTACCAGGCATACGAGTGAATCTTCTGTCTATGCAACTGAGGAGGAAGAAGATGAATATGGAGCCAAAATACAGCTAGGTCCTATGGTCACTATTAAAGAACACCTTGAGAAAGATAAGGTTTTGTTTCTTTTGGAACTACACATTTGTCTTGTTAATATAATGTTTAATTCCTAAGTAAATTACTTAAATTACATTTTTCATGCTTTATTTTTTGTTCATTGTTTATGTTTTTGGGTTGTACGAATGAATATAGGACGATGAGAGTCTAAGGAAATGGAAGGAGCAGCTTCTTGGGAGTGTCGATGTCAACAACATTGGAGGTATATAAATCACGGTTGTCTCTTTTATAACACTATCATGTCTGATTACAATTGTCACCTTTGTAAAAGAAAATTGTTCAAAATCAACGTCACTTTCAATCTTTATTGTAATATTAATTATTCTTTTTATAATTATATATTATAATTAATATTGTGTACATTACTCTCAAGTGAATATCAACTATTTACGTTTTTTTAATTTGTGTGAAATAAACAAGTGTGTCGATTGTTGCAGGGCGAATCGAGTATGTGTTTATGCATGATTGATGAATATGTTCATTTTGAGAATTTACTTATTTTTTTCAATTTATTTGTTTGTAGAAATTCTTGAACCTGAAGTGAATTTTACAAGCCTTTCTATAATCTCTCCTGGTAGAGATGACATTGTTCTTCCCATTCCTGAAGATGGAAAACCACAGGGATTGTGGTTTACTCTTAAAGAAGGTAGTCCTTACCGTCTGAAATTCAGCTTTGTTGTGAGCAATAACATTGTTTCTGGGTTAAAATACACCAATACTGTTTGGAAGACTGCTGTTAAGGGTATGTTGAATTTAATTCATATAGTCTAATGTTTTCCAAATTTCCAATTTTGTAACACTTATCCCTTTTAATTTTAATTGTATTAAAACAAATGTTATTTCAGTGGATAGTTCAAAAGAGATGCTTGGAACTTTCAGCCCTCAACCAGAGCCTTACACACATGAGATGCCAGAAGAAGTCACACCCTCTGGGATATTTGCTAGGGGACAATATTCTGCAAGATCAAAGGTAATTTAGATTGCAATTAACTGCACTGATTTTGATTGAACAATTATAACATATATGCACTTTCATAATTAGATGAAATGTTCCTCATAATTATTTATTGGTAACTCCGTTACATATTAAGTGTAACAATTTCTCTCAAAAAAAAAATAAGTGTAACAATTATTACATTTAAAATATTGTATAATTTTTTTGTTGGAAGATAATGATACATATTTTTCTTGTGCAGTTTCTGGATGATGACAATAAATGTTACTTGGAGATCAACTATACTTTTGATATTAGGAAGGATTGGGCTTGA

Protein sequence

>MS.gene70446.t1

MGSDNENKETGQTSEAGGEKEEHHEPLTRHTSESSVYATEEEEDEYGAKIQLGPMVTIKEHLEKDKDDESLRKWKEQLLGSVDVNNIGEILEPEVNFTSLSIISPGRDDIVLPIPEDGKPQGLWFTLKEGSPYRLKFSFVVSNNIVSGLKYTNTVWKTAVKVDSSKEMLGTFSPQPEPYTHEMPEEVTPSGIFARGQYSARSKFLDDDNKCYLEINYTFDIRKDWA