AlfalfaGEDB | MS.gene71952

M. sativa cultivar XinJiangDaYe / MS.gene71952

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71952.t1	RHN63233.1	88.7	186	16	3	1	186	135	315	1.40E-60	242.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71952.t1	Q9M202	37.0	184	97	4	11	183	111	286	9.9e-23	108.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71952.t1	G7JIV8	89.2	186	15	3	1	186	135	315	1.0e-60	242.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene71952.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene71952	0.83207	1.17E-55	-1.69E-46
MS.gene049404	MS.gene71952	0.815978	7.01E-52	-1.69E-46
MS.gene050561	MS.gene71952	0.80334	3.65E-49	-1.69E-46
MS.gene050759	MS.gene71952	0.810955	8.91E-51	-1.69E-46
MS.gene050962	MS.gene71952	0.803684	3.10E-49	-1.69E-46
MS.gene051247	MS.gene71952	0.800809	1.21E-48	-1.69E-46
MS.gene051279	MS.gene71952	0.832002	1.21E-55	-1.69E-46
MS.gene051744	MS.gene71952	0.855853	4.77E-62	-1.69E-46
MS.gene052488	MS.gene71952	0.811103	8.27E-51	-1.69E-46
MS.gene052582	MS.gene71952	0.817544	3.12E-52	-1.69E-46
MS.gene052584	MS.gene71952	0.837936	3.87E-57	-1.69E-46
MS.gene053226	MS.gene71952	0.840051	1.10E-57	-1.69E-46
MS.gene053296	MS.gene71952	0.8043	2.31E-49	-1.69E-46
MS.gene053621	MS.gene71952	0.801595	8.36E-49	-1.69E-46
MS.gene054576	MS.gene71952	0.815704	8.07E-52	-1.69E-46
MS.gene054651	MS.gene71952	0.852093	5.79E-61	-1.69E-46
MS.gene055586	MS.gene71952	0.809273	2.05E-50	-1.69E-46
MS.gene055589	MS.gene71952	0.807038	6.14E-50	-1.69E-46
MS.gene056050	MS.gene71952	0.828342	9.48E-55	-1.69E-46
MS.gene056174	MS.gene71952	0.804401	2.20E-49	-1.69E-46
MS.gene05686	MS.gene71952	0.807622	4.62E-50	-1.69E-46
MS.gene057444	MS.gene71952	0.848076	7.72E-60	-1.69E-46
MS.gene058010	MS.gene71952	0.803485	3.41E-49	-1.69E-46
MS.gene058625	MS.gene71952	0.804871	1.75E-49	-1.69E-46
MS.gene058979	MS.gene71952	0.807297	5.42E-50	-1.69E-46
MS.gene059601	MS.gene71952	0.847772	9.36E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71952.t1	MTR_4g100860	94.624	186	5	3	1	186	135	315	1.39e-125	356
MS.gene71952.t1	MTR_0160s0030	40.000	215	86	6	7	186	149	355	6.19e-35	126
MS.gene71952.t1	MTR_5g012630	39.130	161	49	5	11	171	148	259	9.83e-24	94.7
MS.gene71952.t1	MTR_4g027440	32.075	212	87	5	20	174	135	346	1.98e-20	87.4
MS.gene71952.t1	MTR_4g109340	50.000	64	26	2	116	179	436	493	9.76e-12	63.2
MS.gene71952.t1	MTR_4g082980	50.000	62	27	1	115	176	393	450	1.02e-11	62.8
MS.gene71952.t1	MTR_1g106730	31.548	168	75	6	24	176	57	199	1.41e-11	61.6
MS.gene71952.t1	MTR_3g102980	29.762	168	84	5	24	172	49	201	4.67e-11	60.1
MS.gene71952.t1	MTR_1g018420	28.916	166	89	4	24	172	40	193	6.36e-11	59.7
MS.gene71952.t1	MTR_7g100100	33.898	118	65	3	73	178	32	148	7.38e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71952.t1	AT3G60580	40.741	189	83	6	11	183	111	286	4.64e-35	124
MS.gene71952.t1	AT2G45120	41.000	200	92	6	1	185	124	312	1.08e-32	119
MS.gene71952.t1	AT1G02030	38.710	186	73	6	10	183	110	266	2.50e-21	88.6
MS.gene71952.t1	AT1G26610	44.595	74	36	1	103	176	387	455	3.18e-13	67.4
MS.gene71952.t1	AT5G61470	43.902	82	36	3	69	140	218	299	4.55e-12	63.5
MS.gene71952.t1	AT3G53600	34.545	110	59	4	77	177	51	156	5.03e-11	58.9
MS.gene71952.t1	AT3G49930	32.576	132	60	4	22	138	55	172	8.51e-11	59.3

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAACACCTTAAAACAAAA+TGG	0.311153	8.3:+20213695	None:intergenic
TCTTTAAATCTAGCAGCAAT+AGG	0.373006	8.3:+20213581	None:intergenic
CACATAGCAACATCTTCTTC+AGG	0.374474	8.3:+20213980	None:intergenic
TGATGCTTTCGCGAGACAAA+TGG	0.443203	8.3:-20213952	MS.gene71952:CDS
GTGAATTGTGATGGAGTTGT+TGG	0.452696	8.3:+20214040	None:intergenic
GTTTCGATCTTCAAGAGCAT+TGG	0.459910	8.3:-20213798	MS.gene71952:CDS
TTTCGATCTTCAAGAGCATT+GGG	0.469548	8.3:-20213797	MS.gene71952:CDS
TTTGGTTCTGGTCAAGCACT+TGG	0.469573	8.3:-20213677	MS.gene71952:CDS
GGTTCTGGTCAAGCACTTGG+TGG	0.484093	8.3:-20213674	MS.gene71952:CDS
TGAAGAAGAAGAGGGATCGG+TGG	0.488736	8.3:-20213891	MS.gene71952:CDS
TCATCTTCTTCAAGTGGAGC+TGG	0.500458	8.3:+20213536	None:intergenic
GCGAAGAATGGTAATGGTAA+TGG	0.505680	8.3:-20213743	MS.gene71952:CDS
ACATAGCAACATCTTCTTCA+GGG	0.525812	8.3:+20213981	None:intergenic
AAGTTATTGAAACGAGTTCG+TGG	0.531632	8.3:-20213851	MS.gene71952:CDS
GCAGTGGAAGTTGATGAAGA+AGG	0.533864	8.3:+20213626	None:intergenic
TGTCGCGATGAAGCGAAGAA+TGG	0.548591	8.3:-20213755	MS.gene71952:CDS
TCTTGTTCAGTGAATTGTGA+TGG	0.549593	8.3:+20214031	None:intergenic
CAGTGGAAGTTGATGAAGAA+GGG	0.550853	8.3:+20213627	None:intergenic
GGAACAAGATGAAGAAGAAG+AGG	0.558902	8.3:-20213900	MS.gene71952:CDS
AGATCATCATCTTCTTCAAG+TGG	0.559232	8.3:+20213530	None:intergenic
GATGAAGCGAAGAATGGTAA+TGG	0.574456	8.3:-20213749	MS.gene71952:CDS
ATTAACATTAACATTAGCAG+TGG	0.584695	8.3:+20213610	None:intergenic
AGATGAAGAAGAAGAGGGAT+CGG	0.594748	8.3:-20213894	MS.gene71952:CDS
AGTTATTGAAACGAGTTCGT+GGG	0.596995	8.3:-20213850	MS.gene71952:CDS
GTATCAGAAACTGAACTCAC+AGG	0.612015	8.3:+20214007	None:intergenic
CATAGCAACATCTTCTTCAG+GGG	0.618873	8.3:+20213982	None:intergenic
GAACAAGATGAAGAAGAAGA+GGG	0.633530	8.3:-20213899	MS.gene71952:CDS
AGCATTTGTGTGAAAACTGT+GGG	0.639279	8.3:-20213826	MS.gene71952:CDS
ATCGGTGGAGAAAATATCGA+AGG	0.639870	8.3:-20213876	MS.gene71952:CDS
GATGAACAACATCAACAATG+TGG	0.658258	8.3:-20213921	MS.gene71952:CDS
AAGCATTTGTGTGAAAACTG+TGG	0.714401	8.3:-20213827	MS.gene71952:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTAACATTAACATTAGCAG+TGG	+	chr8.3:20213947-20213966	None:intergenic	25.0%
!!!	TGAATGTCCATTTTGTTTTA+AGG	-	chr8.3:20213852-20213871	MS.gene71952:CDS	25.0%
	CCAAACACCTTAAAACAAAA+TGG	+	chr8.3:20213862-20213881	None:intergenic	30.0%
	TCTTTAAATCTAGCAGCAAT+AGG	+	chr8.3:20213976-20213995	None:intergenic	30.0%
!!!	CCATTTTGTTTTAAGGTGTT+TGG	-	chr8.3:20213859-20213878	MS.gene71952:CDS	30.0%
	AAGCATTTGTGTGAAAACTG+TGG	-	chr8.3:20213727-20213746	MS.gene71952:CDS	35.0%
	AAGTTATTGAAACGAGTTCG+TGG	-	chr8.3:20213703-20213722	MS.gene71952:CDS	35.0%
	ACATAGCAACATCTTCTTCA+GGG	+	chr8.3:20213576-20213595	None:intergenic	35.0%
	AGATCATCATCTTCTTCAAG+TGG	+	chr8.3:20214027-20214046	None:intergenic	35.0%
	AGCATTTGTGTGAAAACTGT+GGG	-	chr8.3:20213728-20213747	MS.gene71952:CDS	35.0%
	AGTTATTGAAACGAGTTCGT+GGG	-	chr8.3:20213704-20213723	MS.gene71952:CDS	35.0%
	GAACAAGATGAAGAAGAAGA+GGG	-	chr8.3:20213655-20213674	MS.gene71952:CDS	35.0%
	GATGAACAACATCAACAATG+TGG	-	chr8.3:20213633-20213652	MS.gene71952:CDS	35.0%
	TCTTGTTCAGTGAATTGTGA+TGG	+	chr8.3:20213526-20213545	None:intergenic	35.0%
!!	TTTCGATCTTCAAGAGCATT+GGG	-	chr8.3:20213757-20213776	MS.gene71952:CDS	35.0%
!!!	TGTTTTAAGGTGTTTGGTTC+TGG	-	chr8.3:20213865-20213884	MS.gene71952:CDS	35.0%
	AGATGAAGAAGAAGAGGGAT+CGG	-	chr8.3:20213660-20213679	MS.gene71952:CDS	40.0%
	ATCGGTGGAGAAAATATCGA+AGG	-	chr8.3:20213678-20213697	MS.gene71952:CDS	40.0%
	CACATAGCAACATCTTCTTC+AGG	+	chr8.3:20213577-20213596	None:intergenic	40.0%
	CATAGCAACATCTTCTTCAG+GGG	+	chr8.3:20213575-20213594	None:intergenic	40.0%
	GATGAAGCGAAGAATGGTAA+TGG	-	chr8.3:20213805-20213824	MS.gene71952:CDS	40.0%
	GGAACAAGATGAAGAAGAAG+AGG	-	chr8.3:20213654-20213673	MS.gene71952:CDS	40.0%
	GTATCAGAAACTGAACTCAC+AGG	+	chr8.3:20213550-20213569	None:intergenic	40.0%
!	GTGAATTGTGATGGAGTTGT+TGG	+	chr8.3:20213517-20213536	None:intergenic	40.0%
!	GTTTCGATCTTCAAGAGCAT+TGG	-	chr8.3:20213756-20213775	MS.gene71952:CDS	40.0%
!!	CAGTGGAAGTTGATGAAGAA+GGG	+	chr8.3:20213930-20213949	None:intergenic	40.0%
!!	GCGAAGAATGGTAATGGTAA+TGG	-	chr8.3:20213811-20213830	MS.gene71952:CDS	40.0%
	TCATCTTCTTCAAGTGGAGC+TGG	+	chr8.3:20214021-20214040	None:intergenic	45.0%
	TGATGCTTTCGCGAGACAAA+TGG	-	chr8.3:20213602-20213621	MS.gene71952:CDS	45.0%
!!	GCAGTGGAAGTTGATGAAGA+AGG	+	chr8.3:20213931-20213950	None:intergenic	45.0%
!!	TTTGGTTCTGGTCAAGCACT+TGG	-	chr8.3:20213877-20213896	MS.gene71952:CDS	45.0%
	TGAAGAAGAAGAGGGATCGG+TGG	-	chr8.3:20213663-20213682	MS.gene71952:CDS	50.0%
	TGTCGCGATGAAGCGAAGAA+TGG	-	chr8.3:20213799-20213818	MS.gene71952:CDS	50.0%
!	GGTTCTGGTCAAGCACTTGG+TGG	-	chr8.3:20213880-20213899	MS.gene71952:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	20213508	20214068	20213508	ID=MS.gene71952
chr8.3	mRNA	20213508	20214068	20213508	ID=MS.gene71952.t1;Parent=MS.gene71952
chr8.3	exon	20213508	20214068	20213508	ID=MS.gene71952.t1.exon1;Parent=MS.gene71952.t1
chr8.3	CDS	20213508	20214068	20213508	ID=cds.MS.gene71952.t1;Parent=MS.gene71952.t1

Gene Sequence

>MS.gene71952

ATGGTGCCAACAACTCCATCACAATTCACTGAACAAGAACCTGTGAGTTCAGTTTCTGATACTTCCCCTGAAGAAGATGTTGCTATGTGTCTCATGATGCTTTCGCGAGACAAATGGAGTAGAAAGATGAACAACATCAACAATGTGGAACAAGATGAAGAAGAAGAGGGATCGGTGGAGAAAATATCGAAGGTGAAGTTATTGAAACGAGTTCGTGGGAAGCATTTGTGTGAAAACTGTGGGAAGACGTTTCGATCTTCAAGAGCATTGGGAAGTCATAGAAGTATTTGTTGTCGCGATGAAGCGAAGAATGGTAATGGTAATGGTAAAGATGATAAGATTTTTGAATGTCCATTTTGTTTTAAGGTGTTTGGTTCTGGTCAAGCACTTGGTGGTCACAAGAGATCTCATCTCATGATCCCTTCTTCATCAACTTCCACTGCTAATGTTAATGTTAATGTTAATCCTATTGCTGCTAGATTTAAAGAGAGTTTCATAGATCTCAATATGCCAGCTCCACTTGAAGAAGATGATGATCTTAGTGTTGTTTCTGATGCCTAA

Protein sequence

>MS.gene71952.t1

MVPTTPSQFTEQEPVSSVSDTSPEEDVAMCLMMLSRDKWSRKMNNINNVEQDEEEEGSVEKISKVKLLKRVRGKHLCENCGKTFRSSRALGSHRSICCRDEAKNGNGNGKDDKIFECPFCFKVFGSGQALGGHKRSHLMIPSSSTSTANVNVNVNPIAARFKESFIDLNMPAPLEEDDDLSVVSDA