Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72697.t1 | XP_013453611.1 | 98.6 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 4.80E-69 | 270.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72697.t1 | I3SJ84 | 98.6 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 3.5e-69 | 270.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049419 | MS.gene72697 | -0.804107 | 2.53E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene72697 | 0.812868 | 3.41E-51 | -1.69E-46 |
| MS.gene050105 | MS.gene72697 | 0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene051066 | MS.gene72697 | 0.800185 | 1.62E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene72697 | 0.815451 | 9.19E-52 | -1.69E-46 |
| MS.gene052954 | MS.gene72697 | 0.820008 | 8.61E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene72697 | 0.829442 | 5.14E-55 | -1.69E-46 |
| MS.gene053008 | MS.gene72697 | 0.816603 | 5.08E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene72697 | 0.816773 | 4.65E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene72697 | 0.806957 | 6.39E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene72697 | 0.825624 | 4.23E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene72697 | 0.819545 | 1.10E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene72697 | 0.81743 | 3.31E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene72697 | 0.804769 | 1.84E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene72697 | 0.82615 | 3.18E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene72697 | 0.839072 | 1.97E-57 | -1.69E-46 |
| MS.gene058783 | MS.gene72697 | 0.815415 | 9.35E-52 | -1.69E-46 |
| MS.gene059830 | MS.gene72697 | 0.804396 | 2.20E-49 | -1.69E-46 |
| MS.gene059952 | MS.gene72697 | 0.818469 | 1.93E-52 | -1.69E-46 |
| MS.gene060527 | MS.gene72697 | 0.820139 | 8.03E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene72697 | 0.804447 | 2.15E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene72697 | 0.809817 | 1.57E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene72697 | 0.834738 | 2.52E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene72697 | 0.824573 | 7.50E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72697.t1 | MTR_5g025015 | 98.601 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 8.78e-99 | 280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72697.t1 | AT3G51500 | 50.000 | 138 | 47 | 5 | 1 | 138 | 1 | 116 | 5.79e-26 | 95.1 |
Find 44 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAAGTAGAGATGCTTTC+TGG | 0.155423 | 5.4:+16188679 | None:intergenic |
| TTCAGTCATCAAGTGTCTTT+TGG | 0.232137 | 5.4:+16188590 | None:intergenic |
| GAGGAAGATGGCTTTGTATT+AGG | 0.253421 | 5.4:-16188459 | MS.gene72697:CDS |
| AGAGGAGAAGATTGCATTGC+TGG | 0.335131 | 5.4:-16188394 | MS.gene72697:CDS |
| GAATCTGAATTGGAACCTTC+TGG | 0.388596 | 5.4:+16188546 | None:intergenic |
| GCTAAAAGAAGCTGAAGATC+AGG | 0.403208 | 5.4:-16188430 | MS.gene72697:CDS |
| GCTCCTCTTCCGTTGAAATC+AGG | 0.411458 | 5.4:-16188624 | MS.gene72697:CDS |
| TTGAAGTAGAGATGCTTTCT+GGG | 0.412709 | 5.4:+16188680 | None:intergenic |
| GTCGGGCAACGAATCTGAAT+TGG | 0.413676 | 5.4:+16188536 | None:intergenic |
| TCCTCTGACAAAGGAATCCT+TGG | 0.414586 | 5.4:+16188741 | None:intergenic |
| ATGCAATCTTCTCCTCTTCA+AGG | 0.425654 | 5.4:+16188400 | None:intergenic |
| GGAAGGCCTTGTTGATCCCT+TGG | 0.442315 | 5.4:-16188355 | MS.gene72697:CDS |
| GGAAATATTGTCAAGGGAGG+GGG | 0.456451 | 5.4:-16188651 | MS.gene72697:CDS |
| GAACCTTCTGGTCTCTGAAA+AGG | 0.466094 | 5.4:+16188558 | None:intergenic |
| TTTCCACCCGCAGAGGAGTC+GGG | 0.472445 | 5.4:+16188519 | None:intergenic |
| AATACTTCCGCCTCAATTGA+CGG | 0.473041 | 5.4:+16188490 | None:intergenic |
| CTCAATATCTCCTCTGACAA+AGG | 0.476118 | 5.4:+16188732 | None:intergenic |
| TGTCCTGATTTCAACGGAAG+AGG | 0.492333 | 5.4:+16188621 | None:intergenic |
| ATTTCCACCCGCAGAGGAGT+CGG | 0.498474 | 5.4:+16188518 | None:intergenic |
| GCTGGATCAACTTGTTGTAA+TGG | 0.502973 | 5.4:-16188376 | MS.gene72697:CDS |
| AGGAAATATTGTCAAGGGAG+GGG | 0.510018 | 5.4:-16188652 | MS.gene72697:CDS |
| TCGTTGCCCGACTCCTCTGC+GGG | 0.538190 | 5.4:-16188525 | MS.gene72697:CDS |
| GAGGCGGAAGTATTTGCAGC+TGG | 0.549105 | 5.4:-16188481 | MS.gene72697:CDS |
| TTGCCCGACTCCTCTGCGGG+TGG | 0.564389 | 5.4:-16188522 | MS.gene72697:CDS |
| ACACTATGAGCGAATCTCCA+AGG | 0.573497 | 5.4:+16188338 | None:intergenic |
| GAAATGAATACCGTCAATTG+AGG | 0.576227 | 5.4:-16188500 | MS.gene72697:CDS |
| TATTTGCAGCTGGAGGAAGA+TGG | 0.578348 | 5.4:-16188471 | MS.gene72697:CDS |
| TGCAATCTTCTCCTCTTCAA+GGG | 0.582806 | 5.4:+16188401 | None:intergenic |
| CCTTCTGGTCTCTGAAAAGG+TGG | 0.583162 | 5.4:+16188561 | None:intergenic |
| TTCGTTGCCCGACTCCTCTG+CGG | 0.588076 | 5.4:-16188526 | MS.gene72697:CDS |
| TCAGGTAAAGACCCTTGAAG+AGG | 0.601213 | 5.4:-16188412 | MS.gene72697:CDS |
| GGTGAGAAATGCAAGATCCA+AGG | 0.602347 | 5.4:-16188758 | None:intergenic |
| TCCAAGGATTCCTTTGTCAG+AGG | 0.602608 | 5.4:-16188742 | MS.gene72697:CDS |
| CACTATGAGCGAATCTCCAA+GGG | 0.604058 | 5.4:+16188339 | None:intergenic |
| CTGCAAGGAAATATTGTCAA+GGG | 0.605688 | 5.4:-16188657 | MS.gene72697:CDS |
| AAGGAAATATTGTCAAGGGA+GGG | 0.608332 | 5.4:-16188653 | MS.gene72697:CDS |
| GATCAACTTGTTGTAATGGA+AGG | 0.608527 | 5.4:-16188372 | MS.gene72697:CDS |
| ACTGCAAGGAAATATTGTCA+AGG | 0.612756 | 5.4:-16188658 | MS.gene72697:CDS |
| CAAGGAAATATTGTCAAGGG+AGG | 0.627083 | 5.4:-16188654 | MS.gene72697:CDS |
| GCATCTCTACTTCAACTGCA+AGG | 0.630440 | 5.4:-16188672 | MS.gene72697:CDS |
| ATGAATACCGTCAATTGAGG+CGG | 0.653058 | 5.4:-16188497 | MS.gene72697:CDS |
| GCGGAAGTATTTGCAGCTGG+AGG | 0.666953 | 5.4:-16188478 | MS.gene72697:CDS |
| GTATTCATTTCCACCCGCAG+AGG | 0.686415 | 5.4:+16188512 | None:intergenic |
| GAATCTCCAAGGGATCAACA+AGG | 0.729425 | 5.4:+16188349 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTGAGTTTTTCTTCTTCTT+TGG | + | chr5.4:16188392-16188411 | None:intergenic | 30.0% |
| ! | TTTTTGTGTCCTGATTTCAA+CGG | + | chr5.4:16188479-16188498 | None:intergenic | 30.0% |
| AAGGAAATATTGTCAAGGGA+GGG | - | chr5.4:16188438-16188457 | MS.gene72697:CDS | 35.0% | |
| ACTGCAAGGAAATATTGTCA+AGG | - | chr5.4:16188433-16188452 | MS.gene72697:CDS | 35.0% | |
| CTGCAAGGAAATATTGTCAA+GGG | - | chr5.4:16188434-16188453 | MS.gene72697:CDS | 35.0% | |
| GAAATGAATACCGTCAATTG+AGG | - | chr5.4:16188591-16188610 | MS.gene72697:CDS | 35.0% | |
| TTGAAGTAGAGATGCTTTCT+GGG | + | chr5.4:16188414-16188433 | None:intergenic | 35.0% | |
| ! | TTCAGTCATCAAGTGTCTTT+TGG | + | chr5.4:16188504-16188523 | None:intergenic | 35.0% |
| !! | GATCAACTTGTTGTAATGGA+AGG | - | chr5.4:16188719-16188738 | MS.gene72697:CDS | 35.0% |
| AATACTTCCGCCTCAATTGA+CGG | + | chr5.4:16188604-16188623 | None:intergenic | 40.0% | |
| AGGAAATATTGTCAAGGGAG+GGG | - | chr5.4:16188439-16188458 | MS.gene72697:CDS | 40.0% | |
| ATGAATACCGTCAATTGAGG+CGG | - | chr5.4:16188594-16188613 | MS.gene72697:CDS | 40.0% | |
| ATGCAATCTTCTCCTCTTCA+AGG | + | chr5.4:16188694-16188713 | None:intergenic | 40.0% | |
| CAAGGAAATATTGTCAAGGG+AGG | - | chr5.4:16188437-16188456 | MS.gene72697:CDS | 40.0% | |
| CTCAATATCTCCTCTGACAA+AGG | + | chr5.4:16188362-16188381 | None:intergenic | 40.0% | |
| GAATCTGAATTGGAACCTTC+TGG | + | chr5.4:16188548-16188567 | None:intergenic | 40.0% | |
| GCTAAAAGAAGCTGAAGATC+AGG | - | chr5.4:16188661-16188680 | MS.gene72697:CDS | 40.0% | |
| GTTGAAGTAGAGATGCTTTC+TGG | + | chr5.4:16188415-16188434 | None:intergenic | 40.0% | |
| TGCAATCTTCTCCTCTTCAA+GGG | + | chr5.4:16188693-16188712 | None:intergenic | 40.0% | |
| !! | GAGGAAGATGGCTTTGTATT+AGG | - | chr5.4:16188632-16188651 | MS.gene72697:CDS | 40.0% |
| !! | GCTGGATCAACTTGTTGTAA+TGG | - | chr5.4:16188715-16188734 | MS.gene72697:CDS | 40.0% |
| AGAGGAGAAGATTGCATTGC+TGG | - | chr5.4:16188697-16188716 | MS.gene72697:CDS | 45.0% | |
| GAATCTCCAAGGGATCAACA+AGG | + | chr5.4:16188745-16188764 | None:intergenic | 45.0% | |
| GCATCTCTACTTCAACTGCA+AGG | - | chr5.4:16188419-16188438 | MS.gene72697:CDS | 45.0% | |
| GGAAATATTGTCAAGGGAGG+GGG | - | chr5.4:16188440-16188459 | MS.gene72697:CDS | 45.0% | |
| TATTTGCAGCTGGAGGAAGA+TGG | - | chr5.4:16188620-16188639 | MS.gene72697:CDS | 45.0% | |
| TCAGGTAAAGACCCTTGAAG+AGG | - | chr5.4:16188679-16188698 | MS.gene72697:CDS | 45.0% | |
| TCCAAGGATTCCTTTGTCAG+AGG | - | chr5.4:16188349-16188368 | MS.gene72697:CDS | 45.0% | |
| TCCTCTGACAAAGGAATCCT+TGG | + | chr5.4:16188353-16188372 | None:intergenic | 45.0% | |
| TGTCCTGATTTCAACGGAAG+AGG | + | chr5.4:16188473-16188492 | None:intergenic | 45.0% | |
| ! | GAACCTTCTGGTCTCTGAAA+AGG | + | chr5.4:16188536-16188555 | None:intergenic | 45.0% |
| GCTCCTCTTCCGTTGAAATC+AGG | - | chr5.4:16188467-16188486 | MS.gene72697:CDS | 50.0% | |
| GTATTCATTTCCACCCGCAG+AGG | + | chr5.4:16188582-16188601 | None:intergenic | 50.0% | |
| GTCGGGCAACGAATCTGAAT+TGG | + | chr5.4:16188558-16188577 | None:intergenic | 50.0% | |
| ! | CCACCTTTTCAGAGACCAGA+AGG | - | chr5.4:16188530-16188549 | MS.gene72697:CDS | 50.0% |
| ! | CCTTCTGGTCTCTGAAAAGG+TGG | + | chr5.4:16188533-16188552 | None:intergenic | 50.0% |
| ATTTCCACCCGCAGAGGAGT+CGG | + | chr5.4:16188576-16188595 | None:intergenic | 55.0% | |
| TATTGTCAAGGGAGGGGGAC+AGG | - | chr5.4:16188445-16188464 | MS.gene72697:CDS | 55.0% | |
| !! | GAGGCGGAAGTATTTGCAGC+TGG | - | chr5.4:16188610-16188629 | MS.gene72697:CDS | 55.0% |
| !! | GCGGAAGTATTTGCAGCTGG+AGG | - | chr5.4:16188613-16188632 | MS.gene72697:CDS | 55.0% |
| !! | GGAAGGCCTTGTTGATCCCT+TGG | - | chr5.4:16188736-16188755 | MS.gene72697:CDS | 55.0% |
| TTCGTTGCCCGACTCCTCTG+CGG | - | chr5.4:16188565-16188584 | MS.gene72697:CDS | 60.0% | |
| TTTCCACCCGCAGAGGAGTC+GGG | + | chr5.4:16188575-16188594 | None:intergenic | 60.0% | |
| TCGTTGCCCGACTCCTCTGC+GGG | - | chr5.4:16188566-16188585 | MS.gene72697:CDS | 65.0% | |
| TTGCCCGACTCCTCTGCGGG+TGG | - | chr5.4:16188569-16188588 | MS.gene72697:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 16188341 | 16188772 | 16188341 | ID=MS.gene72697 |
| chr5.4 | mRNA | 16188341 | 16188772 | 16188341 | ID=MS.gene72697.t1;Parent=MS.gene72697 |
| chr5.4 | exon | 16188341 | 16188772 | 16188341 | ID=MS.gene72697.t1.exon1;Parent=MS.gene72697.t1 |
| chr5.4 | CDS | 16188341 | 16188772 | 16188341 | ID=cds.MS.gene72697.t1;Parent=MS.gene72697.t1 |
| Gene Sequence |
| Protein sequence |
