Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72974.t1 | XP_024629609.1 | 81.1 | 106 | 9 | 2 | 1 | 96 | 1 | 105 | 3.10E-35 | 157.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72974.t1 | A0A072VKL8 | 81.1 | 106 | 9 | 2 | 1 | 96 | 1 | 105 | 2.3e-35 | 157.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050397 | MS.gene72974 | 0.824848 | 6.46E-54 | -1.69E-46 |
| MS.gene050971 | MS.gene72974 | 0.814982 | 1.17E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene72974 | 0.804421 | 2.18E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene72974 | -0.818159 | 2.27E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene72974 | 0.806348 | 8.59E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene72974 | 0.812483 | 4.14E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene72974 | 0.827134 | 1.85E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene72974 | 0.824958 | 6.09E-54 | -1.69E-46 |
| MS.gene054013 | MS.gene72974 | 0.821707 | 3.50E-53 | -1.69E-46 |
| MS.gene054349 | MS.gene72974 | 0.801047 | 1.08E-48 | -1.69E-46 |
| MS.gene055116 | MS.gene72974 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene055604 | MS.gene72974 | 0.836792 | 7.60E-57 | -1.69E-46 |
| MS.gene056191 | MS.gene72974 | 0.832387 | 9.73E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene72974 | 0.824863 | 6.41E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene72974 | 0.803684 | 3.10E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene72974 | 0.828695 | 7.79E-55 | -1.69E-46 |
| MS.gene057823 | MS.gene72974 | 0.824477 | 7.90E-54 | -1.69E-46 |
| MS.gene057825 | MS.gene72974 | 0.811634 | 6.35E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene72974 | 0.803488 | 3.40E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene72974 | 0.804914 | 1.72E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene72974 | 0.852614 | 4.11E-61 | -1.69E-46 |
| MS.gene059420 | MS.gene72974 | 0.808668 | 2.77E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene72974 | 0.806671 | 7.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72974.t1 | MTR_1g058120 | 81.132 | 106 | 9 | 2 | 1 | 96 | 1 | 105 | 7.35e-53 | 161 |
| MS.gene72974.t1 | MTR_7g105730 | 57.843 | 102 | 18 | 4 | 1 | 92 | 14 | 100 | 1.36e-22 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72974.t1 | AT3G11600 | 47.115 | 104 | 29 | 4 | 1 | 92 | 1 | 90 | 5.81e-18 | 73.2 |
| MS.gene72974.t1 | AT5G06270 | 57.547 | 106 | 29 | 3 | 1 | 96 | 1 | 100 | 1.55e-17 | 72.0 |
| MS.gene72974.t1 | AT5G06270 | 57.547 | 106 | 29 | 3 | 1 | 96 | 1 | 100 | 1.55e-17 | 72.0 |
Find 30 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGCCACTTCCATGGTTT+TGG | 0.229980 | 1.1:-45229220 | MS.gene72974:CDS |
| GCTGGTTGTTTCCATCTTCT+TGG | 0.237222 | 1.1:+45229265 | None:intergenic |
| TTAAGTTTCAAGTCAAGTTT+TGG | 0.305467 | 1.1:+45229396 | None:intergenic |
| AAAACCATGGAAGTGGCTTC+AGG | 0.383022 | 1.1:+45229222 | None:intergenic |
| AAAACAATGAGTCGAAGAAA+TGG | 0.423682 | 1.1:-45229426 | None:intergenic |
| GATACTGTTGCTGATCTTGT+TGG | 0.429438 | 1.1:+45229330 | None:intergenic |
| CTAGCAGAAGAATGCAGAAC+TGG | 0.446661 | 1.1:+45228909 | None:intergenic |
| TTCTGCATTCTTCTGCTAGT+AGG | 0.457170 | 1.1:-45228905 | MS.gene72974:CDS |
| ACTCTTCTGTGGTCAACCCT+TGG | 0.466923 | 1.1:+45229363 | None:intergenic |
| ATCTTCTTGGTTTAGCTCAG+TGG | 0.472861 | 1.1:+45229278 | None:intergenic |
| AAACCATGGAAGTGGCTTCA+GGG | 0.473103 | 1.1:+45229223 | None:intergenic |
| TGTTAGAGTATCTCTGCTGC+TGG | 0.501808 | 1.1:+45229247 | None:intergenic |
| CTTCTGTGGTCAACCCTTGG+TGG | 0.502015 | 1.1:+45229366 | None:intergenic |
| TGGAGACACATGAGCTTGGT+GGG | 0.512861 | 1.1:+45229298 | None:intergenic |
| CATGAGCTTGGTGGGGATGT+AGG | 0.514574 | 1.1:+45229306 | None:intergenic |
| GGTCAACCCTTGGTGGTGAT+AGG | 0.515699 | 1.1:+45229373 | None:intergenic |
| GTGGAGACACATGAGCTTGG+TGG | 0.522809 | 1.1:+45229297 | None:intergenic |
| ACTGAGCTAAACCAAGAAGA+TGG | 0.542560 | 1.1:-45229276 | MS.gene72974:CDS |
| AAGAATGCAGAACTGGCAAG+AGG | 0.552827 | 1.1:+45228916 | None:intergenic |
| CAGCCCTGAAGCCACTTCCA+TGG | 0.556097 | 1.1:-45229226 | MS.gene72974:CDS |
| ACCAACCAAAACCATGGAAG+TGG | 0.557850 | 1.1:+45229215 | None:intergenic |
| TCAGTGGAGACACATGAGCT+TGG | 0.587149 | 1.1:+45229294 | None:intergenic |
| GTGATGACTCAACTCTTCTG+TGG | 0.595889 | 1.1:+45229352 | None:intergenic |
| ATGAGTCGAAGAAATGGAAG+TGG | 0.602019 | 1.1:-45229420 | MS.gene72974:CDS |
| AGGACAACCAACCAAAACCA+TGG | 0.614893 | 1.1:+45229209 | None:intergenic |
| AGAATGCAGAACTGGCAAGA+GGG | 0.630677 | 1.1:+45228917 | None:intergenic |
| AACTTAACCTATCACCACCA+AGG | 0.641567 | 1.1:-45229380 | MS.gene72974:CDS |
| ACTTAACCTATCACCACCAA+GGG | 0.643165 | 1.1:-45229379 | MS.gene72974:CDS |
| GGAGACACATGAGCTTGGTG+GGG | 0.701423 | 1.1:+45229299 | None:intergenic |
| ATGACATACATAAGACAACG+AGG | 0.800120 | 1.1:+45229189 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTAGTTTTTGCCAAAATTA+CGG | - | chr1.1:45229307-45229326 | MS.gene72974:CDS | 25.0% |
| !!! | TTAAGTTTCAAGTCAAGTTT+TGG | + | chr1.1:45228916-45228935 | None:intergenic | 25.0% |
| ATGAAAACAACAACTCCAAA+AGG | - | chr1.1:45229205-45229224 | MS.gene72974:CDS | 30.0% | |
| ! | AAAACAGTGCTTTTGCATTT+AGG | + | chr1.1:45229171-45229190 | None:intergenic | 30.0% |
| ! | AAACAGTGCTTTTGCATTTA+GGG | + | chr1.1:45229170-45229189 | None:intergenic | 30.0% |
| ! | ATGCAGTTAATTCTTCCTTT+TGG | + | chr1.1:45229223-45229242 | None:intergenic | 30.0% |
| ACCTACACTTAATGCATGTT+TGG | + | chr1.1:45229370-45229389 | None:intergenic | 35.0% | |
| ATGACATACATAAGACAACG+AGG | + | chr1.1:45229123-45229142 | None:intergenic | 35.0% | |
| GGAAGAATTAACTGCATGTT+TGG | - | chr1.1:45229226-45229245 | MS.gene72974:CDS | 35.0% | |
| TCCAAACATGCATTAAGTGT+AGG | - | chr1.1:45229366-45229385 | MS.gene72974:CDS | 35.0% | |
| TTGAAGCTTCAACCAAATGA+CGG | + | chr1.1:45229285-45229304 | None:intergenic | 35.0% | |
| !!! | TGGAGTTGTTGTTTTCATGA+TGG | + | chr1.1:45229203-45229222 | None:intergenic | 35.0% |
| AACTTAACCTATCACCACCA+AGG | - | chr1.1:45228929-45228948 | MS.gene72974:intron | 40.0% | |
| ACTGAGCTAAACCAAGAAGA+TGG | - | chr1.1:45229033-45229052 | MS.gene72974:intron | 40.0% | |
| ACTTAACCTATCACCACCAA+GGG | - | chr1.1:45228930-45228949 | MS.gene72974:intron | 40.0% | |
| ATGAGTCGAAGAAATGGAAG+TGG | - | chr1.1:45228889-45228908 | MS.gene72974:CDS | 40.0% | |
| GATACTGTTGCTGATCTTGT+TGG | + | chr1.1:45228982-45229001 | None:intergenic | 40.0% | |
| TTCTGCATTCTTCTGCTAGT+AGG | - | chr1.1:45229404-45229423 | MS.gene72974:CDS | 40.0% | |
| ! | ACTGTGTGCTACCGTAATTT+TGG | + | chr1.1:45229321-45229340 | None:intergenic | 40.0% |
| ! | ATCTTCTTGGTTTAGCTCAG+TGG | + | chr1.1:45229034-45229053 | None:intergenic | 40.0% |
| !! | CTTTTGCATTTAGGGCACTT+TGG | + | chr1.1:45229162-45229181 | None:intergenic | 40.0% |
| AAAACCATGGAAGTGGCTTC+AGG | + | chr1.1:45229090-45229109 | None:intergenic | 45.0% | |
| AAACCATGGAAGTGGCTTCA+GGG | + | chr1.1:45229089-45229108 | None:intergenic | 45.0% | |
| AAGAATGCAGAACTGGCAAG+AGG | + | chr1.1:45229396-45229415 | None:intergenic | 45.0% | |
| ACCAACCAAAACCATGGAAG+TGG | + | chr1.1:45229097-45229116 | None:intergenic | 45.0% | |
| AGAATGCAGAACTGGCAAGA+GGG | + | chr1.1:45229395-45229414 | None:intergenic | 45.0% | |
| AGGACAACCAACCAAAACCA+TGG | + | chr1.1:45229103-45229122 | None:intergenic | 45.0% | |
| CTAGCAGAAGAATGCAGAAC+TGG | + | chr1.1:45229403-45229422 | None:intergenic | 45.0% | |
| GTGATGACTCAACTCTTCTG+TGG | + | chr1.1:45228960-45228979 | None:intergenic | 45.0% | |
| ! | GCTGGTTGTTTCCATCTTCT+TGG | + | chr1.1:45229047-45229066 | None:intergenic | 45.0% |
| ! | TGAAGCCACTTCCATGGTTT+TGG | - | chr1.1:45229089-45229108 | MS.gene72974:intron | 45.0% |
| ! | TGTTAGAGTATCTCTGCTGC+TGG | + | chr1.1:45229065-45229084 | None:intergenic | 45.0% |
| ACTCTTCTGTGGTCAACCCT+TGG | + | chr1.1:45228949-45228968 | None:intergenic | 50.0% | |
| ATCACAGTAGCGCCGTCATT+TGG | - | chr1.1:45229270-45229289 | MS.gene72974:CDS | 50.0% | |
| TCAGTGGAGACACATGAGCT+TGG | + | chr1.1:45229018-45229037 | None:intergenic | 50.0% | |
| TGGAGACACATGAGCTTGGT+GGG | + | chr1.1:45229014-45229033 | None:intergenic | 50.0% | |
| !!! | GCCACTTCCATGGTTTTGGT+TGG | - | chr1.1:45229093-45229112 | MS.gene72974:intron | 50.0% |
| CTTCTGTGGTCAACCCTTGG+TGG | + | chr1.1:45228946-45228965 | None:intergenic | 55.0% | |
| GTGGAGACACATGAGCTTGG+TGG | + | chr1.1:45229015-45229034 | None:intergenic | 55.0% | |
| ! | CATGAGCTTGGTGGGGATGT+AGG | + | chr1.1:45229006-45229025 | None:intergenic | 55.0% |
| ! | GGAGACACATGAGCTTGGTG+GGG | + | chr1.1:45229013-45229032 | None:intergenic | 55.0% |
| !! | GGTCAACCCTTGGTGGTGAT+AGG | + | chr1.1:45228939-45228958 | None:intergenic | 55.0% |
| CAGCCCTGAAGCCACTTCCA+TGG | - | chr1.1:45229083-45229102 | MS.gene72974:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 45228889 | 45229442 | 45228889 | ID=MS.gene72974 |
| chr1.1 | mRNA | 45228889 | 45229442 | 45228889 | ID=MS.gene72974.t1;Parent=MS.gene72974 |
| chr1.1 | exon | 45229198 | 45229442 | 45229198 | ID=MS.gene72974.t1.exon1;Parent=MS.gene72974.t1 |
| chr1.1 | CDS | 45229198 | 45229442 | 45229198 | ID=cds.MS.gene72974.t1;Parent=MS.gene72974.t1 |
| chr1.1 | exon | 45228889 | 45228943 | 45228889 | ID=MS.gene72974.t1.exon2;Parent=MS.gene72974.t1 |
| chr1.1 | CDS | 45228889 | 45228943 | 45228889 | ID=cds.MS.gene72974.t1;Parent=MS.gene72974.t1 |
| Gene Sequence |
| Protein sequence |
