Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73233.t1 | XP_013454934.1 | 79.9 | 284 | 42 | 4 | 1 | 282 | 1 | 271 | 6.10E-111 | 410.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73233.t1 | A0A072UH33 | 79.9 | 284 | 42 | 4 | 1 | 282 | 1 | 271 | 4.4e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene73233 | 0.812163 | 4.87E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene73233 | 0.806534 | 7.85E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene73233 | 0.809324 | 2.00E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene73233 | 0.801656 | 8.12E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene73233 | 0.808702 | 2.72E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene73233 | 0.810267 | 1.26E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene73233 | 0.83031 | 3.16E-55 | -1.69E-46 |
| MS.gene051957 | MS.gene73233 | 0.803365 | 3.61E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene73233 | 0.808168 | 3.54E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene73233 | 0.812477 | 4.16E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene73233 | 0.822239 | 2.63E-53 | -1.69E-46 |
| MS.gene05308 | MS.gene73233 | 0.819412 | 1.18E-52 | -1.69E-46 |
| MS.gene053122 | MS.gene73233 | 0.808086 | 3.68E-50 | -1.69E-46 |
| MS.gene053195 | MS.gene73233 | 0.803603 | 3.22E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene73233 | 0.869229 | 3.62E-66 | -1.69E-46 |
| MS.gene054142 | MS.gene73233 | 0.814721 | 1.33E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene73233 | 0.812207 | 4.76E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene73233 | 0.831303 | 1.80E-55 | -1.69E-46 |
| MS.gene055589 | MS.gene73233 | 0.806079 | 9.79E-50 | -1.69E-46 |
| MS.gene055694 | MS.gene73233 | 0.845898 | 3.05E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene73233 | 0.802501 | 5.45E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene73233 | 0.84077 | 7.12E-58 | -1.69E-46 |
| MS.gene056215 | MS.gene73233 | 0.812353 | 4.42E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene73233 | 0.800474 | 1.42E-48 | -1.69E-46 |
| MS.gene056595 | MS.gene73233 | 0.806498 | 7.99E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene73233 | 0.811301 | 7.50E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene73233 | 0.806139 | 9.51E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene73233 | 0.810035 | 1.41E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene73233 | 0.808478 | 3.04E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene73233 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene73233 | 0.817492 | 3.21E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene73233 | 0.801423 | 9.07E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene73233 | 0.853205 | 2.79E-61 | -1.69E-46 |
| MS.gene059842 | MS.gene73233 | 0.804665 | 1.94E-49 | -1.69E-46 |
| MS.gene059935 | MS.gene73233 | 0.830767 | 2.44E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene73233 | 0.820384 | 7.06E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene73233 | 0.834452 | 2.97E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73233.t1 | MTR_4g019840 | 83.860 | 285 | 31 | 4 | 1 | 283 | 1 | 272 | 8.54e-171 | 475 |
| MS.gene73233.t1 | MTR_0027s0090 | 29.114 | 316 | 167 | 11 | 9 | 285 | 51 | 348 | 3.11e-16 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTTCGTTTCTCAAATT+AGG | 0.099863 | 4.1:+78407389 | None:intergenic |
| TTCTTTCGTTTCTCAAATTA+GGG | 0.263492 | 4.1:+78407390 | None:intergenic |
| AATAACAAAGCCGGATTTCC+TGG | 0.285031 | 4.1:-78407416 | MS.gene73233:CDS |
| TTCTTCCTCCAGGGATATTT+CGG | 0.316300 | 4.1:+78407522 | None:intergenic |
| TTTGAAGAAGGTATGATTAG+AGG | 0.322238 | 4.1:-78407153 | MS.gene73233:CDS |
| TTTCTCAAATTAGGGTTTCC+AGG | 0.367756 | 4.1:+78407398 | None:intergenic |
| TCAGTGTTCCTTCTTCCTCC+AGG | 0.391507 | 4.1:+78407512 | None:intergenic |
| ATTAGGGTTTCCAGGAAATC+CGG | 0.430658 | 4.1:+78407406 | None:intergenic |
| TCATAGGGTATTTCTGAGAA+TGG | 0.439205 | 4.1:-78407983 | MS.gene73233:intron |
| TTGAATCGTAAATTTGAAGA+AGG | 0.448269 | 4.1:-78407165 | MS.gene73233:CDS |
| CAGTGTTCCTTCTTCCTCCA+GGG | 0.484157 | 4.1:+78407513 | None:intergenic |
| GTGATTGAAACGTCGTCGTA+AGG | 0.501542 | 4.1:+78408125 | None:intergenic |
| TGTCTTCATCTGCATCTGCA+TGG | 0.512850 | 4.1:-78407463 | MS.gene73233:CDS |
| CAGCTGCAGGAGAAGATGTA+AGG | 0.516185 | 4.1:-78407880 | MS.gene73233:CDS |
| AAGAGTGGTTGAACTTGAAA+CGG | 0.521960 | 4.1:+78408151 | None:intergenic |
| GGAAATTACCGAAATATCCC+TGG | 0.523195 | 4.1:-78407530 | MS.gene73233:CDS |
| GCTATTTCACAGAGAAGAAG+TGG | 0.536018 | 4.1:-78407551 | MS.gene73233:CDS |
| CTGCAAGTTCTGCTTCAAAA+CGG | 0.546551 | 4.1:+78407928 | None:intergenic |
| GATCTGCTACATGCAGCTGC+AGG | 0.549527 | 4.1:-78407893 | MS.gene73233:CDS |
| AGCTACTTGAAGAGTTCAAG+TGG | 0.554786 | 4.1:-78407959 | MS.gene73233:CDS |
| AGAAACGAAAGAAATGGTAC+TGG | 0.560342 | 4.1:-78407380 | MS.gene73233:CDS |
| GCATGGAGTTCTGTGAAGAA+TGG | 0.561312 | 4.1:-78407446 | MS.gene73233:CDS |
| GAAATATCCCTGGAGGAAGA+AGG | 0.566843 | 4.1:-78407520 | MS.gene73233:CDS |
| GGAAGCAACAATAACAAAGC+CGG | 0.570578 | 4.1:-78407425 | MS.gene73233:CDS |
| AATTTGAGAAACGAAAGAAA+TGG | 0.570863 | 4.1:-78407386 | MS.gene73233:CDS |
| AGGAAGAAGGAACACTGAAA+CGG | 0.589211 | 4.1:-78407507 | MS.gene73233:CDS |
| TTGAGATGTTGAAGAAGCAG+TGG | 0.595071 | 4.1:-78407601 | MS.gene73233:CDS |
| CTCATCTCTCCACAACAACA+AGG | 0.596113 | 4.1:-78408078 | MS.gene73233:intron |
| AGCAGAACTTGCAGAGAGCG+AGG | 0.606981 | 4.1:-78407919 | MS.gene73233:CDS |
| CGTGAAGCAGCAGCTGACAG+AGG | 0.646842 | 4.1:-78406948 | MS.gene73233:CDS |
| GCAGTGGTTGAAGCAACGAG+AGG | 0.691118 | 4.1:-78407585 | MS.gene73233:CDS |
| AATTACCGAAATATCCCTGG+AGG | 0.707791 | 4.1:-78407527 | MS.gene73233:CDS |
| TCAGTAGCATCAACAACACG+AGG | 0.724071 | 4.1:+78407347 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTTTTGTATTGATT+TGG | - | chr4.1:78408113-78408132 | MS.gene73233:CDS | 15.0% |
| !!! | AAGTTTTTTTGTATTGATTT+GGG | - | chr4.1:78408114-78408133 | MS.gene73233:CDS | 15.0% |
| !!! | ATTTTTTTGTTTGTGTTTTT+TGG | - | chr4.1:78407928-78407947 | MS.gene73233:CDS | 15.0% |
| !!! | TTTTTTTGTTTGTGTTTTTT+GGG | - | chr4.1:78407929-78407948 | MS.gene73233:CDS | 15.0% |
| !!! | AGTTTTTTTGTATTGATTTG+GGG | - | chr4.1:78408115-78408134 | MS.gene73233:CDS | 20.0% |
| !!! | GTATTTTTTTCTTCTTCATA+GGG | - | chr4.1:78407167-78407186 | MS.gene73233:CDS | 20.0% |
| !!! | TGTATTTTTTTCTTCTTCAT+AGG | - | chr4.1:78407166-78407185 | MS.gene73233:CDS | 20.0% |
| !!! | TTTTTTGTTTGTGTTTTTTG+GGG | - | chr4.1:78407930-78407949 | MS.gene73233:CDS | 20.0% |
| ! | AATTTGAGAAACGAAAGAAA+TGG | - | chr4.1:78407779-78407798 | MS.gene73233:CDS | 25.0% |
| ! | ACTGAATCAAGAAAAAATTC+AGG | - | chr4.1:78407836-78407855 | MS.gene73233:CDS | 25.0% |
| ! | TTCTTTCGTTTCTCAAATTA+GGG | + | chr4.1:78407778-78407797 | None:intergenic | 25.0% |
| ! | TTGAATCGTAAATTTGAAGA+AGG | - | chr4.1:78408000-78408019 | MS.gene73233:intron | 25.0% |
| ! | TTTCTTTCGTTTCTCAAATT+AGG | + | chr4.1:78407779-78407798 | None:intergenic | 25.0% |
| !! | AATGAAAGATTTTTCGTCTT+CGG | + | chr4.1:78406972-78406991 | None:intergenic | 25.0% |
| !!! | GGAACTTCAAATTTTGAAAA+TGG | - | chr4.1:78408063-78408082 | MS.gene73233:intron | 25.0% |
| !!! | GTTTTTTTGTATTGATTTGG+GGG | - | chr4.1:78408116-78408135 | MS.gene73233:CDS | 25.0% |
| !!! | TGATGTGTTTGTTGTATTTT+AGG | - | chr4.1:78408155-78408174 | MS.gene73233:CDS | 25.0% |
| !!! | TTTGTTGTATTTTAGGTGTT+GGG | - | chr4.1:78408162-78408181 | MS.gene73233:CDS | 25.0% |
| !!! | TTTTTGTTTGTGTTTTTTGG+GGG | - | chr4.1:78407931-78407950 | MS.gene73233:CDS | 25.0% |
| ! | GAAGAAGAAGAAGCTTTTTA+CGG | - | chr4.1:78407326-78407345 | MS.gene73233:intron | 30.0% |
| ! | TTTGAAGAAGGTATGATTAG+AGG | - | chr4.1:78408012-78408031 | MS.gene73233:intron | 30.0% |
| !! | AAAGCATGAACAACTTTTTC+AGG | + | chr4.1:78407982-78408001 | None:intergenic | 30.0% |
| !!! | GTTTGTTGTATTTTAGGTGT+TGG | - | chr4.1:78408161-78408180 | MS.gene73233:CDS | 30.0% |
| !!! | TTTTTGTTACCTTGTTGTTG+TGG | + | chr4.1:78407099-78407118 | None:intergenic | 30.0% |
| AAGAGTGGTTGAACTTGAAA+CGG | + | chr4.1:78407017-78407036 | None:intergenic | 35.0% | |
| AGAAACGAAAGAAATGGTAC+TGG | - | chr4.1:78407785-78407804 | MS.gene73233:CDS | 35.0% | |
| TGTAGGTAGTAAAAATGTGG+TGG | - | chr4.1:78407954-78407973 | MS.gene73233:CDS | 35.0% | |
| TTTCTCAAATTAGGGTTTCC+AGG | + | chr4.1:78407770-78407789 | None:intergenic | 35.0% | |
| ! | ATGGATTTTCTCAGCAAAAG+CGG | - | chr4.1:78408192-78408211 | MS.gene73233:CDS | 35.0% |
| ! | TCATAGGGTATTTCTGAGAA+TGG | - | chr4.1:78407182-78407201 | MS.gene73233:CDS | 35.0% |
| !! | AAATCCAGTGTGCTTTTTAG+GGG | + | chr4.1:78407357-78407376 | None:intergenic | 35.0% |
| !! | ACGGTTTTTCAGAGTAATGT+TGG | - | chr4.1:78407677-78407696 | MS.gene73233:intron | 35.0% |
| !! | CAGCAGAAGAATCGTTTTTA+TGG | - | chr4.1:78408042-78408061 | MS.gene73233:intron | 35.0% |
| !! | GAAATCCAGTGTGCTTTTTA+GGG | + | chr4.1:78407358-78407377 | None:intergenic | 35.0% |
| !! | TGAAATCCAGTGTGCTTTTT+AGG | + | chr4.1:78407359-78407378 | None:intergenic | 35.0% |
| AATAACAAAGCCGGATTTCC+TGG | - | chr4.1:78407749-78407768 | MS.gene73233:CDS | 40.0% | |
| AATTACCGAAATATCCCTGG+AGG | - | chr4.1:78407638-78407657 | MS.gene73233:intron | 40.0% | |
| AGCTACTTGAAGAGTTCAAG+TGG | - | chr4.1:78407206-78407225 | MS.gene73233:CDS | 40.0% | |
| AGGAAGAAGGAACACTGAAA+CGG | - | chr4.1:78407658-78407677 | MS.gene73233:intron | 40.0% | |
| ATTAGGGTTTCCAGGAAATC+CGG | + | chr4.1:78407762-78407781 | None:intergenic | 40.0% | |
| CTGCAAGTTCTGCTTCAAAA+CGG | + | chr4.1:78407240-78407259 | None:intergenic | 40.0% | |
| GGAAATTACCGAAATATCCC+TGG | - | chr4.1:78407635-78407654 | MS.gene73233:intron | 40.0% | |
| GGAAGCAACAATAACAAAGC+CGG | - | chr4.1:78407740-78407759 | MS.gene73233:intron | 40.0% | |
| GGGTGTAGGTAGTAAAAATG+TGG | - | chr4.1:78407951-78407970 | MS.gene73233:CDS | 40.0% | |
| TTCTTCCTCCAGGGATATTT+CGG | + | chr4.1:78407646-78407665 | None:intergenic | 40.0% | |
| TTGAGATGTTGAAGAAGCAG+TGG | - | chr4.1:78407564-78407583 | MS.gene73233:CDS | 40.0% | |
| ! | GCTATTTCACAGAGAAGAAG+TGG | - | chr4.1:78407614-78407633 | MS.gene73233:intron | 40.0% |
| ! | TTTACCCCTAAAAAGCACAC+TGG | - | chr4.1:78407350-78407369 | MS.gene73233:CDS | 40.0% |
| !! | TTAGGTGTTGGGAGTGATTA+TGG | - | chr4.1:78408173-78408192 | MS.gene73233:CDS | 40.0% |
| !!! | TTTGTGTTTTTTGGGGGTGT+AGG | - | chr4.1:78407937-78407956 | MS.gene73233:CDS | 40.0% |
| CTCATCTCTCCACAACAACA+AGG | - | chr4.1:78407087-78407106 | MS.gene73233:intron | 45.0% | |
| GAAATATCCCTGGAGGAAGA+AGG | - | chr4.1:78407645-78407664 | MS.gene73233:intron | 45.0% | |
| GCATGGAGTTCTGTGAAGAA+TGG | - | chr4.1:78407719-78407738 | MS.gene73233:intron | 45.0% | |
| GTCGTCGTAAGGAAAAAGAG+TGG | + | chr4.1:78407032-78407051 | None:intergenic | 45.0% | |
| GTGATTGAAACGTCGTCGTA+AGG | + | chr4.1:78407043-78407062 | None:intergenic | 45.0% | |
| TCAGTAGCATCAACAACACG+AGG | + | chr4.1:78407821-78407840 | None:intergenic | 45.0% | |
| TGTCTTCATCTGCATCTGCA+TGG | - | chr4.1:78407702-78407721 | MS.gene73233:intron | 45.0% | |
| CAGCTGCAGGAGAAGATGTA+AGG | - | chr4.1:78407285-78407304 | MS.gene73233:intron | 50.0% | |
| CAGTGTTCCTTCTTCCTCCA+GGG | + | chr4.1:78407655-78407674 | None:intergenic | 50.0% | |
| TCAGTGTTCCTTCTTCCTCC+AGG | + | chr4.1:78407656-78407675 | None:intergenic | 50.0% | |
| AGCAGAACTTGCAGAGAGCG+AGG | - | chr4.1:78407246-78407265 | MS.gene73233:intron | 55.0% | |
| GATCTGCTACATGCAGCTGC+AGG | - | chr4.1:78407272-78407291 | MS.gene73233:intron | 55.0% | |
| GCAGTGGTTGAAGCAACGAG+AGG | - | chr4.1:78407580-78407599 | MS.gene73233:CDS | 55.0% | |
| CGTGAAGCAGCAGCTGACAG+AGG | - | chr4.1:78408217-78408236 | MS.gene73233:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 78406938 | 78408249 | 78406938 | ID=MS.gene73233 |
| chr4.1 | mRNA | 78406938 | 78408249 | 78406938 | ID=MS.gene73233.t1;Parent=MS.gene73233 |
| chr4.1 | exon | 78408079 | 78408249 | 78408079 | ID=MS.gene73233.t1.exon1;Parent=MS.gene73233.t1 |
| chr4.1 | CDS | 78408079 | 78408249 | 78408079 | ID=cds.MS.gene73233.t1;Parent=MS.gene73233.t1 |
| chr4.1 | exon | 78407745 | 78407999 | 78407745 | ID=MS.gene73233.t1.exon2;Parent=MS.gene73233.t1 |
| chr4.1 | CDS | 78407745 | 78407999 | 78407745 | ID=cds.MS.gene73233.t1;Parent=MS.gene73233.t1 |
| chr4.1 | exon | 78407330 | 78407624 | 78407330 | ID=MS.gene73233.t1.exon3;Parent=MS.gene73233.t1 |
| chr4.1 | CDS | 78407330 | 78407624 | 78407330 | ID=cds.MS.gene73233.t1;Parent=MS.gene73233.t1 |
| chr4.1 | exon | 78407103 | 78407228 | 78407103 | ID=MS.gene73233.t1.exon4;Parent=MS.gene73233.t1 |
| chr4.1 | CDS | 78407103 | 78407228 | 78407103 | ID=cds.MS.gene73233.t1;Parent=MS.gene73233.t1 |
| chr4.1 | exon | 78406938 | 78406981 | 78406938 | ID=MS.gene73233.t1.exon5;Parent=MS.gene73233.t1 |
| chr4.1 | CDS | 78406938 | 78406981 | 78406938 | ID=cds.MS.gene73233.t1;Parent=MS.gene73233.t1 |
| Gene Sequence |
| Protein sequence |
