AlfalfaGEDB | MS.gene93131

M. sativa cultivar XinJiangDaYe / MS.gene93131

KEGG: K04797
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF02996.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93131.t1	XP_003592733.1	99.3	152	1	0	1	152	1	152	2.90E-72	281.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93131.t1	P57742	71.1	152	41	1	1	152	1	149	1.8e-51	203.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93131.t1	G7IE99	99.3	152	1	0	1	152	1	152	2.1e-72	281.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene93131	MS.gene26565	PPI
MS.gene009509	MS.gene93131	PPI
MS.gene93131	MS.gene75567	PPI
MS.gene51967	MS.gene93131	PPI
MS.gene93131	MS.gene69947	PPI
MS.gene93131	MS.gene09543	PPI
MS.gene67725	MS.gene93131	PPI
MS.gene93131	MS.gene47513	PPI
MS.gene51964	MS.gene93131	PPI
MS.gene065547	MS.gene93131	PPI
MS.gene002306	MS.gene93131	PPI
MS.gene02265	MS.gene93131	PPI
MS.gene93131	MS.gene058245	PPI
MS.gene92650	MS.gene93131	PPI
MS.gene000365	MS.gene93131	PPI
MS.gene93131	MS.gene79126	PPI
MS.gene061811	MS.gene93131	PPI
MS.gene20245	MS.gene93131	PPI
MS.gene93131	MS.gene75501	PPI
MS.gene045227	MS.gene93131	PPI
MS.gene41343	MS.gene93131	PPI
MS.gene67743	MS.gene93131	PPI
MS.gene93131	MS.gene84699	PPI
MS.gene73500	MS.gene93131	PPI
MS.gene93131	MS.gene09540	PPI
MS.gene65650	MS.gene93131	PPI
MS.gene63427	MS.gene93131	PPI
MS.gene93131	MS.gene01697	PPI
MS.gene53708	MS.gene93131	PPI
MS.gene27709	MS.gene93131	PPI
MS.gene045141	MS.gene93131	PPI
MS.gene51687	MS.gene93131	PPI
MS.gene93131	MS.gene52223	PPI
MS.gene93131	MS.gene019081	PPI
MS.gene93131	MS.gene023855	PPI
MS.gene93131	MS.gene57325	PPI
MS.gene015202	MS.gene93131	PPI
MS.gene93131	MS.gene43369	PPI
MS.gene008730	MS.gene93131	PPI
MS.gene28046	MS.gene93131	PPI
MS.gene009372	MS.gene93131	PPI
MS.gene01284	MS.gene93131	PPI
MS.gene93131	MS.gene63795	PPI
MS.gene51515	MS.gene93131	PPI
MS.gene55980	MS.gene93131	PPI
MS.gene93131	MS.gene00146	PPI
MS.gene43992	MS.gene93131	PPI
MS.gene030840	MS.gene93131	PPI
MS.gene95934	MS.gene93131	PPI
MS.gene93131	MS.gene79100	PPI
MS.gene015206	MS.gene93131	PPI
MS.gene067039	MS.gene93131	PPI
MS.gene93131	MS.gene005125	PPI
MS.gene93131	MS.gene39588	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93131.t1	MTR_1g114500	99.342	152	1	0	1	152	1	152	8.12e-109	306
MS.gene93131.t1	MTR_1g114500	90.345	145	4	1	1	145	1	135	6.83e-91	260

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93131.t1	AT5G23290	72.368	152	39	2	1	152	1	149	2.79e-73	216

Find 29 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCACGTTCTCGTCGATAT+CGG	0.346200	1.1:+80440440	MS.gene93131:CDS
AGTTAAGGAACAAGCAGATA+TGG	0.392343	1.1:+80440253	MS.gene93131:CDS
TCGCAGTAATCCTTTCCTTC+AGG	0.397139	1.1:-80440604	None:intergenic
TGAACAGTTGAAAGCAGTTA+AGG	0.432363	1.1:+80440238	MS.gene93131:CDS
CGGAAGCGGAGCGATGACTC+TGG	0.463988	1.1:+80440202	MS.gene93131:CDS
CAAGACAGTCTCAACAATAT+CGG	0.466377	1.1:+80440290	MS.gene93131:CDS
GATCTCTCTCTCCGTTCTCT+CGG	0.470701	1.1:+80440356	MS.gene93131:CDS
TGTTGAGACTGTCTTGAAGA+AGG	0.480129	1.1:-80440283	None:intergenic
GTTCTCGTCGATATCGGAAC+TGG	0.508671	1.1:+80440446	MS.gene93131:CDS
TGAGGATCTTGAAGAGTAGC+AGG	0.512402	1.1:-80440419	None:intergenic
GAGATCGTTGAGAGCGGTGG+TGG	0.515505	1.1:-80440339	None:intergenic
GAAAACCATGCCTGAAGGAA+AGG	0.522733	1.1:+80440594	MS.gene93131:intron
GCTTCGTCGAAGAGCGGAAG+CGG	0.528323	1.1:+80440188	MS.gene93131:CDS
AAAACGTTGCGGATGAAGCT+GGG	0.534177	1.1:+80441002	MS.gene93131:CDS
GTAATCCTTTCCTTCAGGCA+TGG	0.536343	1.1:-80440599	None:intergenic
TAACTTCGATCAACTTGTTG+AGG	0.544631	1.1:+80440651	MS.gene93131:CDS
GAGTTCAAGACGAGTGGTGG+CGG	0.544654	1.1:-80440315	None:intergenic
GGCGAGTTCAAGACGAGTGG+TGG	0.560196	1.1:-80440318	None:intergenic
AGAGAGATCGTTGAGAGCGG+TGG	0.573255	1.1:-80440342	None:intergenic
GAGAAGCGGTGAGAGGAACA+AGG	0.602439	1.1:-80440388	None:intergenic
AAAATGGCTTCGTCGAAGAG+CGG	0.608939	1.1:+80440182	None:intergenic
ATATCGACGAGAACGTGATG+AGG	0.609043	1.1:-80440437	None:intergenic
ACATAGAGAGAAGCGGTGAG+AGG	0.615765	1.1:-80440395	None:intergenic
AAACAGAAAACCATGCCTGA+AGG	0.621995	1.1:+80440589	MS.gene93131:intron
AGCAGGAACATAGAGAGAAG+CGG	0.640463	1.1:-80440402	None:intergenic
GCGGAGCGATGACTCTGGAG+AGG	0.643180	1.1:+80440207	MS.gene93131:CDS
GAGAGAGAGATCGTTGAGAG+CGG	0.661545	1.1:-80440345	None:intergenic
CGGAACTGGTTATTTCGTCG+AGG	0.673821	1.1:+80440460	MS.gene93131:CDS
GGTGGCGAGTTCAAGACGAG+TGG	0.675476	1.1:-80440321	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAGATCTAAATAAATTAAT+CGG	-	chr1.1:80440503-80440522	None:intergenic	15.0%
!!	AAAAATATCAAAGAGAAAAC+TGG	-	chr1.1:80440908-80440927	None:intergenic	20.0%
!!	AGATCTAAATAAATTAATCG+GGG	-	chr1.1:80440501-80440520	None:intergenic	20.0%
!!	CAGATCTAAATAAATTAATC+GGG	-	chr1.1:80440502-80440521	None:intergenic	20.0%
!!	CTAGACAAAATATCAAATTA+GGG	-	chr1.1:80440873-80440892	None:intergenic	20.0%
!	CCTAGACAAAATATCAAATT+AGG	-	chr1.1:80440874-80440893	None:intergenic	25.0%
!!	ATCGTATATGCTTCTTTTTA+GGG	+	chr1.1:80440523-80440542	MS.gene93131:intron	25.0%
!!	CCTAATTTGATATTTTGTCT+AGG	+	chr1.1:80440871-80440890	MS.gene93131:intron	25.0%
!!	TATCGTATATGCTTCTTTTT+AGG	+	chr1.1:80440522-80440541	MS.gene93131:intron	25.0%
!!!	GTGTTAGTTTTATACTAATG+TGG	+	chr1.1:80440796-80440815	MS.gene93131:intron	25.0%
	CATGATGTAATTACAAATGC+AGG	+	chr1.1:80440964-80440983	MS.gene93131:intron	30.0%
!!!	TATGCTTCTTTTTAGGGTTA+TGG	+	chr1.1:80440529-80440548	MS.gene93131:intron	30.0%
	AGTTAAGGAACAAGCAGATA+TGG	+	chr1.1:80440253-80440272	MS.gene93131:CDS	35.0%
	CAAGACAGTCTCAACAATAT+CGG	+	chr1.1:80440290-80440309	MS.gene93131:CDS	35.0%
	CTAATGTGGTTATTCTGTTG+TGG	+	chr1.1:80440810-80440829	MS.gene93131:intron	35.0%
	TGAACAGTTGAAAGCAGTTA+AGG	+	chr1.1:80440238-80440257	MS.gene93131:CDS	35.0%
!!	TAACTTCGATCAACTTGTTG+AGG	+	chr1.1:80440651-80440670	MS.gene93131:CDS	35.0%
	AAACAGAAAACCATGCCTGA+AGG	+	chr1.1:80440589-80440608	MS.gene93131:intron	40.0%
	GTCCAAGAAGAAAAACGTTG+CGG	+	chr1.1:80440991-80441010	MS.gene93131:CDS	40.0%
	TGTTGAGACTGTCTTGAAGA+AGG	-	chr1.1:80440286-80440305	None:intergenic	40.0%
!	ATCCGCAACGTTTTTCTTCT+TGG	-	chr1.1:80440996-80441015	None:intergenic	40.0%
!	GGATTTTACTACCGAGAGAA+CGG	-	chr1.1:80440370-80440389	None:intergenic	40.0%
!!!	TGAAGCTGGGCTTATTTTAC+AGG	+	chr1.1:80441015-80441034	MS.gene93131:CDS	40.0%
	AAAAACGTTGCGGATGAAGC+TGG	+	chr1.1:80441001-80441020	MS.gene93131:CDS	45.0%
	AAAACGTTGCGGATGAAGCT+GGG	+	chr1.1:80441002-80441021	MS.gene93131:CDS	45.0%
	AGCAGGAACATAGAGAGAAG+CGG	-	chr1.1:80440405-80440424	None:intergenic	45.0%
	ATATCGACGAGAACGTGATG+AGG	-	chr1.1:80440440-80440459	None:intergenic	45.0%
	CATCACGTTCTCGTCGATAT+CGG	+	chr1.1:80440440-80440459	MS.gene93131:CDS	45.0%
	GAAAACCATGCCTGAAGGAA+AGG	+	chr1.1:80440594-80440613	MS.gene93131:intron	45.0%
	GTAATCCTTTCCTTCAGGCA+TGG	-	chr1.1:80440602-80440621	None:intergenic	45.0%
	TCGCAGTAATCCTTTCCTTC+AGG	-	chr1.1:80440607-80440626	None:intergenic	45.0%
	TGAGGATCTTGAAGAGTAGC+AGG	-	chr1.1:80440422-80440441	None:intergenic	45.0%
	ACATAGAGAGAAGCGGTGAG+AGG	-	chr1.1:80440398-80440417	None:intergenic	50.0%
	GAGAGAGAGATCGTTGAGAG+CGG	-	chr1.1:80440348-80440367	None:intergenic	50.0%
	GATCTCTCTCTCCGTTCTCT+CGG	+	chr1.1:80440356-80440375	MS.gene93131:CDS	50.0%
	GTTCTCGTCGATATCGGAAC+TGG	+	chr1.1:80440446-80440465	MS.gene93131:CDS	50.0%
!	CGGAACTGGTTATTTCGTCG+AGG	+	chr1.1:80440460-80440479	MS.gene93131:CDS	50.0%
	AGAGAGATCGTTGAGAGCGG+TGG	-	chr1.1:80440345-80440364	None:intergenic	55.0%
	GAGAAGCGGTGAGAGGAACA+AGG	-	chr1.1:80440391-80440410	None:intergenic	55.0%
	GAGTTCAAGACGAGTGGTGG+CGG	-	chr1.1:80440318-80440337	None:intergenic	55.0%
	GAGATCGTTGAGAGCGGTGG+TGG	-	chr1.1:80440342-80440361	None:intergenic	60.0%
	GCTTCGTCGAAGAGCGGAAG+CGG	+	chr1.1:80440188-80440207	MS.gene93131:CDS	60.0%
	GGCGAGTTCAAGACGAGTGG+TGG	-	chr1.1:80440321-80440340	None:intergenic	60.0%
	GGTGGCGAGTTCAAGACGAG+TGG	-	chr1.1:80440324-80440343	None:intergenic	60.0%
	CGGAAGCGGAGCGATGACTC+TGG	+	chr1.1:80440202-80440221	MS.gene93131:CDS	65.0%
!	GCGGAGCGATGACTCTGGAG+AGG	+	chr1.1:80440207-80440226	MS.gene93131:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	80440185	80441069	80440185	ID=MS.gene93131
chr1.1	mRNA	80440185	80441069	80440185	ID=MS.gene93131.t1;Parent=MS.gene93131
chr1.1	exon	80440185	80440481	80440185	ID=MS.gene93131.t1.exon1;Parent=MS.gene93131.t1
chr1.1	CDS	80440185	80440481	80440185	ID=cds.MS.gene93131.t1;Parent=MS.gene93131.t1
chr1.1	exon	80440595	80440672	80440595	ID=MS.gene93131.t1.exon2;Parent=MS.gene93131.t1
chr1.1	CDS	80440595	80440672	80440595	ID=cds.MS.gene93131.t1;Parent=MS.gene93131.t1
chr1.1	exon	80440986	80441069	80440986	ID=MS.gene93131.t1.exon3;Parent=MS.gene93131.t1
chr1.1	CDS	80440986	80441069	80440986	ID=cds.MS.gene93131.t1;Parent=MS.gene93131.t1

Gene Sequence

>MS.gene93131

ATGGCTTCGTCGAAGAGCGGAAGCGGAGCGATGACTCTGGAGAGGATGAGCGTTGAACAGTTGAAAGCAGTTAAGGAACAAGCAGATATGGAAGTTAACCTTCTTCAAGACAGTCTCAACAATATCGGCACCGCCACCACTCGTCTTGAACTCGCCACCACCGCTCTCAACGATCTCTCTCTCCGTTCTCTCGGTAGTAAAATCCTTGTTCCTCTCACCGCTTCTCTCTATGTTCCTGCTACTCTTCAAGATCCTCATCACGTTCTCGTCGATATCGGAACTGGTTATTTCGTCGAGGTGAGCTCAACTCTTTCCCCGATTAATTTATTTAGATCTGTATCGTATATGCTTCTTTTTAGGGTTATGGAATATTAATTTTAATTTGAATGTTGTGTTGATGTTGAAAACAGAAAACCATGCCTGAAGGAAAGGATTACTGCGAGCGCAAAATCAACTTACTCAAATCTAACTTCGATCAACTTGTTGAGGTAATTAATTTTCTTATTAATTAAGTACTCTATTTGATTTTGATATATGTATTTCTACTGAATTTGTTTATTCACGATATATATAGATTATACTAGAATAATTGTCATTTGTACTCGATCGTTGTGTTAGTTTTATACTAATGTGGTTATTCTGTTGTGGAGAGTGATTATTCAAATAACACTTATTTTAGTTTAAACCCTAATTTGATATTTTGTCTAGGAAATGAGCTTACCAGTTTTCTCTTTGATATTTTTATCTCAACTATAATGACATCGTGTTCAAGAAAAAATCATGATGTAATTACAAATGCAGGTTGCGTCCAAGAAGAAAAACGTTGCGGATGAAGCTGGGCTTATTTTACAGGCAAAATTGAAGCAATTAGCATCTTCATCCTAA

Protein sequence

>MS.gene93131.t1

MASSKSGSGAMTLERMSVEQLKAVKEQADMEVNLLQDSLNNIGTATTRLELATTALNDLSLRSLGSKILVPLTASLYVPATLQDPHHVLVDIGTGYFVEKTMPEGKDYCERKINLLKSNFDQLVEVASKKKNVADEAGLILQAKLKQLASSS