Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76962.t1 | XP_003615184.1 | 88.6 | 394 | 37 | 2 | 1 | 394 | 12 | 397 | 8.30E-156 | 560.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76962.t1 | Q9QYW0 | 28.8 | 312 | 181 | 8 | 93 | 365 | 183 | 492 | 1.1e-23 | 112.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76962.t1 | G7KAI8 | 88.6 | 394 | 37 | 2 | 1 | 394 | 12 | 397 | 6.0e-156 | 560.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76962 | MS.gene65479 | PPI |
| MS.gene76962 | MS.gene28303 | PPI |
| MS.gene76962 | MS.gene038409 | PPI |
| MS.gene76962 | MS.gene056617 | PPI |
| MS.gene76962 | MS.gene063568 | PPI |
| MS.gene76962 | MS.gene45448 | PPI |
| MS.gene76962 | MS.gene58159 | PPI |
| MS.gene062301 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene048301 | PPI |
| MS.gene01984 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene70303 | PPI |
| MS.gene28304 | MS.gene76962 | PPI |
| MS.gene063209 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene065496 | PPI |
| MS.gene76962 | MS.gene88378 | PPI |
| MS.gene76962 | MS.gene053381 | PPI |
| MS.gene031356 | MS.gene76962 | PPI |
| MS.gene02854 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene003175 | PPI |
| MS.gene030707 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene003846 | PPI |
| MS.gene029825 | MS.gene76962 | PPI |
| MS.gene017174 | MS.gene76962 | PPI |
| MS.gene76962 | MS.gene28161 | PPI |
| MS.gene75013 | MS.gene76962 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76962.t1 | MTR_7g007890 | 96.450 | 338 | 12 | 0 | 57 | 394 | 60 | 397 | 0.0 | 675 |
| MS.gene76962.t1 | MTR_5g064850 | 96.429 | 336 | 12 | 0 | 59 | 394 | 62 | 397 | 0.0 | 675 |
| MS.gene76962.t1 | MTR_5g064850 | 96.131 | 336 | 11 | 1 | 59 | 394 | 62 | 395 | 0.0 | 667 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76962.t1 | AT5G61330 | 61.413 | 368 | 124 | 7 | 30 | 393 | 83 | 436 | 5.56e-143 | 414 |
| MS.gene76962.t1 | AT5G61330 | 61.413 | 368 | 124 | 7 | 30 | 393 | 83 | 436 | 5.56e-143 | 414 |
| MS.gene76962.t1 | AT5G61330 | 61.413 | 368 | 124 | 7 | 30 | 393 | 94 | 447 | 5.92e-143 | 414 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 7.2:-90006647 | MS.gene76962:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 7.2:-90006388 | MS.gene76962:CDS |
| TGAAAAGATAGTAAACTTTA+TGG | 0.190174 | 7.2:-90004439 | MS.gene76962:CDS |
| TTTAGAAGATTCTCACAATT+TGG | 0.194443 | 7.2:-90007083 | MS.gene76962:CDS |
| ACCGGTGCTGCTGCAATTAA+AGG | 0.225926 | 7.2:-90006582 | MS.gene76962:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 7.2:-90008786 | MS.gene76962:CDS |
| CATCTGTTTGATCATTCTAC+TGG | 0.287484 | 7.2:+90006420 | None:intergenic |
| ATGAATTGCTACCTCGTTAT+AGG | 0.297845 | 7.2:+90011510 | None:intergenic |
| CATGAAGAACTCACCATTAC+TGG | 0.322112 | 7.2:+90008154 | None:intergenic |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 7.2:-90008798 | MS.gene76962:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 7.2:+90006449 | None:intergenic |
| AATCTTACAGCTCCTGTTCC+TGG | 0.355537 | 7.2:+90011248 | None:intergenic |
| TCAGAATCGTCCAGAAGTTC+AGG | 0.374459 | 7.2:+90006005 | None:intergenic |
| ATGCTATTTGATGATCTGTT+TGG | 0.374783 | 7.2:-90004381 | MS.gene76962:CDS |
| TGACGGTGATCCTGAACTTC+TGG | 0.375086 | 7.2:-90006015 | MS.gene76962:CDS |
| TGATAGCGAGTTGGAGGAGG+AGG | 0.397733 | 7.2:-90011401 | MS.gene76962:CDS |
| AGCCATTCTCAAATTCAAAT+AGG | 0.416090 | 7.2:-90008985 | MS.gene76962:CDS |
| ACAACTTGAGAAAGAGTATA+GGG | 0.419407 | 7.2:-90011281 | MS.gene76962:CDS |
| TGAGGGAAGTGACGAGGAAG+AGG | 0.420724 | 7.2:-90011350 | MS.gene76962:CDS |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 7.2:-90009444 | MS.gene76962:CDS |
| GAATCAGACGAGACAGTTAG+AGG | 0.432355 | 7.2:-90008839 | MS.gene76962:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 7.2:-90006646 | MS.gene76962:intron |
| GCCATGGGAGGAAGATTTGC+CGG | 0.449865 | 7.2:+90004408 | None:intergenic |
| AGCCTATTTGAATTTGAGAA+TGG | 0.451198 | 7.2:+90008983 | None:intergenic |
| TCCTTTAATTGCAGCAGCAC+CGG | 0.452449 | 7.2:+90006581 | None:intergenic |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 7.2:-90011305 | MS.gene76962:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 7.2:-90005948 | MS.gene76962:intron |
| AGCAGAAGAAGATGATGGAC+AGG | 0.458004 | 7.2:-90011374 | MS.gene76962:CDS |
| GATGAGGGGCAAGAGGAAAG+TGG | 0.465440 | 7.2:-90011325 | MS.gene76962:CDS |
| AAGAGTGGTCACAGATTTCT+CGG | 0.468215 | 7.2:-90007045 | MS.gene76962:CDS |
| ATCAAATAGCATTGGCGCCA+TGG | 0.471359 | 7.2:+90004392 | None:intergenic |
| TGATGATAGCGAGTTGGAGG+AGG | 0.473123 | 7.2:-90011404 | MS.gene76962:CDS |
| GGATCTTCATCACCAGGAAC+AGG | 0.478572 | 7.2:-90011260 | MS.gene76962:CDS |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 7.2:+90008790 | None:intergenic |
| TAATGATAGCTCTAATGAAC+CGG | 0.480771 | 7.2:-90006260 | MS.gene76962:intron |
| CACAGCCAACCGTGATTATG+AGG | 0.481456 | 7.2:+90004329 | None:intergenic |
| GAAGATGATGGACAGGATGA+GGG | 0.488543 | 7.2:-90011367 | MS.gene76962:CDS |
| AGAAGATGATGGACAGGATG+AGG | 0.489022 | 7.2:-90011368 | MS.gene76962:CDS |
| AGAGGAAGATGAGGGGCAAG+AGG | 0.494847 | 7.2:-90011332 | MS.gene76962:CDS |
| AAACTTTATGGCTCCTCGAC+CGG | 0.496516 | 7.2:-90004427 | MS.gene76962:CDS |
| GGGACAAGTCAATTAACAAA+TGG | 0.500647 | 7.2:-90006626 | MS.gene76962:CDS |
| AGGATGAAGATCTCCTGAAA+GGG | 0.503386 | 7.2:-90009424 | MS.gene76962:CDS |
| GATAGCGAGTTGGAGGAGGA+GGG | 0.511948 | 7.2:-90011400 | MS.gene76962:CDS |
| TAATGATGATAGCGAGTTGG+AGG | 0.512371 | 7.2:-90011407 | MS.gene76962:CDS |
| ATCTGTTTGATCATTCTACT+GGG | 0.515066 | 7.2:+90006421 | None:intergenic |
| AACAACTTGAGAAAGAGTAT+AGG | 0.516904 | 7.2:-90011282 | MS.gene76962:CDS |
| TGACGAGGAAGAGGAAGATG+AGG | 0.520219 | 7.2:-90011341 | MS.gene76962:CDS |
| AGGATGACACAAGTGACAAC+CGG | 0.520387 | 7.2:-90006600 | MS.gene76962:CDS |
| ACCGGCAAATCTTCCTCCCA+TGG | 0.522237 | 7.2:-90004409 | MS.gene76962:CDS |
| AGATTTCTCGGATGCACAGC+AGG | 0.523736 | 7.2:-90007033 | MS.gene76962:intron |
| TCAAATAGCATTGGCGCCAT+GGG | 0.534558 | 7.2:+90004393 | None:intergenic |
| GGAGGAAGATTTGCCGGTCG+AGG | 0.535642 | 7.2:+90004414 | None:intergenic |
| TTCTATATTGGAACTTCAAG+AGG | 0.536268 | 7.2:-90008774 | MS.gene76962:intron |
| AACAGATCATCAAATAGCAT+TGG | 0.546718 | 7.2:+90004384 | None:intergenic |
| GGTTAATGATGATAGCGAGT+TGG | 0.548944 | 7.2:-90011410 | MS.gene76962:CDS |
| AGGGGCAAGAGGAAAGTGGA+TGG | 0.557551 | 7.2:-90011321 | MS.gene76962:CDS |
| GGAGAAGCAGAAGAAGATGA+TGG | 0.561538 | 7.2:-90011379 | MS.gene76962:CDS |
| TTACTGGCTTGAGCAACTGA+TGG | 0.562545 | 7.2:+90008170 | None:intergenic |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 7.2:-90008807 | MS.gene76962:CDS |
| AAGGATGAAGATCTCCTGAA+AGG | 0.581931 | 7.2:-90009425 | MS.gene76962:CDS |
| TCAGTTGCTCAAGCCAGTAA+TGG | 0.587160 | 7.2:-90008167 | MS.gene76962:intron |
| GACGAGGAAGAGGAAGATGA+GGG | 0.588247 | 7.2:-90011340 | MS.gene76962:CDS |
| AATAGCATTGGCGCCATGGG+AGG | 0.603352 | 7.2:+90004396 | None:intergenic |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 7.2:+90006267 | None:intergenic |
| AAATTCAAATAGGCTACCCC+AGG | 0.616563 | 7.2:-90008975 | MS.gene76962:intron |
| GTATAGGGATCTTCATCACC+AGG | 0.617577 | 7.2:-90011266 | MS.gene76962:CDS |
| AGTCAATTAACAAATGGCAG+AGG | 0.624449 | 7.2:-90006620 | MS.gene76962:CDS |
| ATGACAATCTTGATCAAGAG+TGG | 0.634506 | 7.2:-90007060 | MS.gene76962:CDS |
| CTTATGTTTCCAGGATGCCG+AGG | 0.658722 | 7.2:-90006284 | MS.gene76962:intron |
| ACGAGGAAGAGGAAGATGAG+GGG | 0.663371 | 7.2:-90011339 | MS.gene76962:CDS |
| ACAGGATGAGGGAAGTGACG+AGG | 0.677129 | 7.2:-90011356 | MS.gene76962:CDS |
| GCAAGCTGTGAAAAGCCAGA+AGG | 0.680270 | 7.2:-90009402 | MS.gene76962:intron |
| TGATGATATGTCCTATAACG+AGG | 0.728032 | 7.2:-90011521 | MS.gene76962:intron |
| GCTCAGGAAACACATGCTGA+CGG | 0.737841 | 7.2:-90006032 | MS.gene76962:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 90004344 | 90011587 | 90004344 | ID=MS.gene76962 |
| chr7.2 | mRNA | 90004344 | 90011587 | 90004344 | ID=MS.gene76962.t1;Parent=MS.gene76962 |
| chr7.2 | exon | 90011522 | 90011587 | 90011522 | ID=MS.gene76962.t1.exon1;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90011522 | 90011587 | 90011522 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90011256 | 90011431 | 90011256 | ID=MS.gene76962.t1.exon2;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90011256 | 90011431 | 90011256 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90009403 | 90009475 | 90009403 | ID=MS.gene76962.t1.exon3;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90009403 | 90009475 | 90009403 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90008976 | 90009050 | 90008976 | ID=MS.gene76962.t1.exon4;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90008976 | 90009050 | 90008976 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90008775 | 90008885 | 90008775 | ID=MS.gene76962.t1.exon5;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90008775 | 90008885 | 90008775 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90008168 | 90008210 | 90008168 | ID=MS.gene76962.t1.exon6;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90008168 | 90008210 | 90008168 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90007034 | 90007127 | 90007034 | ID=MS.gene76962.t1.exon7;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90007034 | 90007127 | 90007034 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90006560 | 90006662 | 90006560 | ID=MS.gene76962.t1.exon8;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90006560 | 90006662 | 90006560 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90006384 | 90006479 | 90006384 | ID=MS.gene76962.t1.exon9;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90006384 | 90006479 | 90006384 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90006261 | 90006293 | 90006261 | ID=MS.gene76962.t1.exon10;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90006261 | 90006293 | 90006261 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90005949 | 90006048 | 90005949 | ID=MS.gene76962.t1.exon11;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90005949 | 90006048 | 90005949 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90004571 | 90004655 | 90004571 | ID=MS.gene76962.t1.exon12;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90004571 | 90004655 | 90004571 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| chr7.2 | exon | 90004344 | 90004473 | 90004344 | ID=MS.gene76962.t1.exon13;Parent=MS.gene76962.t1 |
| chr7.2 | CDS | 90004344 | 90004473 | 90004344 | ID=cds.MS.gene76962.t1;Parent=MS.gene76962.t1 |
| Gene Sequence |
| Protein sequence |
