Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78362.t1 | AES67726.2 | 79.9 | 219 | 37 | 3 | 1 | 219 | 1 | 212 | 1.10E-86 | 330.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78362.t1 | O23116 | 53.1 | 64 | 28 | 1 | 3 | 64 | 16 | 79 | 1.3e-08 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78362.t1 | G7IUG1 | 79.9 | 219 | 37 | 3 | 1 | 219 | 1 | 212 | 7.8e-87 | 330.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene78362 | 0.807631 | 4.60E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene78362 | 0.83708 | 6.42E-57 | -1.69E-46 |
| MS.gene050043 | MS.gene78362 | 0.801791 | 7.62E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene78362 | 0.800019 | 1.75E-48 | -1.69E-46 |
| MS.gene051279 | MS.gene78362 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene78362 | 0.802876 | 4.56E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene78362 | -0.815219 | 1.03E-51 | -1.69E-46 |
| MS.gene052447 | MS.gene78362 | 0.803778 | 2.96E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene78362 | 0.811286 | 7.55E-51 | -1.69E-46 |
| MS.gene05329 | MS.gene78362 | 0.831931 | 1.26E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene78362 | 0.831472 | 1.64E-55 | -1.69E-46 |
| MS.gene054576 | MS.gene78362 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene78362 | 0.821601 | 3.70E-53 | -1.69E-46 |
| MS.gene055228 | MS.gene78362 | 0.822676 | 2.09E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene78362 | 0.821123 | 4.77E-53 | -1.69E-46 |
| MS.gene056272 | MS.gene78362 | 0.821075 | 4.90E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene78362 | 0.810332 | 1.22E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene78362 | 0.80183 | 7.48E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene78362 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene060257 | MS.gene78362 | 0.811392 | 7.16E-51 | -1.69E-46 |
| MS.gene060373 | MS.gene78362 | 0.800665 | 1.30E-48 | -1.69E-46 |
| MS.gene060521 | MS.gene78362 | 0.807062 | 6.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78362.t1 | MTR_2g098450 | 90.868 | 219 | 13 | 3 | 1 | 219 | 1 | 212 | 7.55e-142 | 402 |
| MS.gene78362.t1 | MTR_2g098470 | 69.231 | 156 | 44 | 4 | 65 | 219 | 467 | 619 | 7.38e-67 | 223 |
| MS.gene78362.t1 | MTR_2g098470 | 74.194 | 62 | 16 | 0 | 3 | 64 | 16 | 77 | 5.08e-20 | 92.4 |
| MS.gene78362.t1 | MTR_2g011420 | 85.484 | 62 | 6 | 2 | 231 | 292 | 1 | 59 | 2.51e-26 | 105 |
| MS.gene78362.t1 | MTR_2g011420 | 85.484 | 62 | 6 | 2 | 231 | 292 | 1 | 59 | 3.06e-25 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78362.t1 | AT1G73730 | 53.125 | 64 | 28 | 1 | 3 | 64 | 16 | 79 | 1.35e-12 | 69.7 |
| MS.gene78362.t1 | AT1G73730 | 53.125 | 64 | 28 | 1 | 3 | 64 | 4 | 67 | 1.36e-12 | 69.7 |
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAACATGTCATTTGATTT+TGG | 0.165378 | 2.3:+4916184 | MS.gene78362:CDS |
| CCATTCTGTGGCTGCCTAAT+TGG | 0.194936 | 2.3:-4916046 | None:intergenic |
| TGCAAACAACATAGTTTCTT+TGG | 0.197966 | 2.3:+4915853 | MS.gene78362:CDS |
| ATGGCTGAAATGGAAGAAAT+TGG | 0.229190 | 2.3:+4914898 | MS.gene78362:CDS |
| GGAGTGAGGGACGGCGGCGG+CGG | 0.256932 | 2.3:+4921731 | MS.gene78362:intron |
| AGAGATTGAAGTTGAAGATT+TGG | 0.271801 | 2.3:+4915700 | MS.gene78362:CDS |
| ACCCACAAATGGAAATTTCT+TGG | 0.278625 | 2.3:-4918393 | None:intergenic |
| AAATTACCCCACGCAGATTC+TGG | 0.280776 | 2.3:-4918317 | None:intergenic |
| GACCATATTCCGCGACTCTA+AGG | 0.291033 | 2.3:-4915964 | None:intergenic |
| GCAGAATAAAGAACCGGTTT+TGG | 0.296006 | 2.3:+4918265 | MS.gene78362:CDS |
| GGCCCATGAAATCATCTAAA+AGG | 0.309800 | 2.3:-4916237 | None:intergenic |
| CAAACCTATCCAGCTCGTTT+TGG | 0.310243 | 2.3:-4916150 | None:intergenic |
| GTTGTGATTGTGGTGGTGTT+AGG | 0.316103 | 2.3:+4921895 | MS.gene78362:CDS |
| GACGGCGGCGGCGGGAGGTC+GGG | 0.337985 | 2.3:+4921740 | MS.gene78362:CDS |
| ATCATTTCATCGTCACTCAT+AGG | 0.349939 | 2.3:-4916259 | None:intergenic |
| GCAGCAGCAATCTTCTATTC+AGG | 0.365842 | 2.3:+4918361 | MS.gene78362:CDS |
| GGACGGCGGCGGCGGGAGGT+CGG | 0.369298 | 2.3:+4921739 | MS.gene78362:CDS |
| ATCTTCATAGATGGCTGAAA+TGG | 0.383245 | 2.3:+4914888 | None:intergenic |
| GACCTTAGAGTCGCGGAATA+TGG | 0.394312 | 2.3:+4915962 | MS.gene78362:CDS |
| ATTTCCATTTGTGGGTGATA+AGG | 0.396431 | 2.3:+4918400 | MS.gene78362:CDS |
| ACAAATGCAATTGTTATTAG+TGG | 0.399312 | 2.3:-4919987 | None:intergenic |
| TGAGGGACGGCGGCGGCGGG+AGG | 0.402695 | 2.3:+4921735 | MS.gene78362:intron |
| CCATTGTCTAGCCAACAAAA+TGG | 0.403643 | 2.3:+4920026 | MS.gene78362:CDS |
| GCAGCAGCATCAGCATCTGC+AGG | 0.404522 | 2.3:+4916000 | MS.gene78362:CDS |
| GAGTGAGGGACGGCGGCGGC+GGG | 0.407373 | 2.3:+4921732 | MS.gene78362:intron |
| GAGGGAGTGAGGGACGGCGG+CGG | 0.409363 | 2.3:+4921728 | MS.gene78362:intron |
| CAAAACGAGCTGGATAGGTT+TGG | 0.420296 | 2.3:+4916151 | MS.gene78362:CDS |
| GCCAAGAAATTTCCATTTGT+GGG | 0.422209 | 2.3:+4918392 | MS.gene78362:CDS |
| GACGAAGAAGGCTTGAGAAT+GGG | 0.426187 | 2.3:+4921961 | MS.gene78362:CDS |
| ATCAATGTTCACGGCTGGGC+TGG | 0.431477 | 2.3:-4916023 | None:intergenic |
| GCAGCTTCATGTGGCATGTC+AGG | 0.432094 | 2.3:-4915929 | None:intergenic |
| TCTTAGATATCACAATATAC+TGG | 0.440458 | 2.3:+4915658 | MS.gene78362:intron |
| TGGCTGAAATGGAAGAAATT+GGG | 0.446661 | 2.3:+4914899 | MS.gene78362:CDS |
| TTGTGATTGTGGTGGTGTTA+GGG | 0.449040 | 2.3:+4921896 | MS.gene78362:CDS |
| AGCCAAGAAATTTCCATTTG+TGG | 0.451303 | 2.3:+4918391 | MS.gene78362:CDS |
| GCGGAGGGAGTGAGGGACGG+CGG | 0.460115 | 2.3:+4921725 | MS.gene78362:intron |
| TCACCTTTCATATTTGCTAC+AGG | 0.465634 | 2.3:-4916082 | None:intergenic |
| ATTGGATCAATGTTCACGGC+TGG | 0.473619 | 2.3:-4916028 | None:intergenic |
| GGACGAAGAAGGCTTGAGAA+TGG | 0.474897 | 2.3:+4921960 | MS.gene78362:CDS |
| CCGTGAACATTGATCCAATT+AGG | 0.480278 | 2.3:+4916032 | MS.gene78362:CDS |
| CGCGGAATATGGTCCTCGTC+AGG | 0.486143 | 2.3:+4915973 | MS.gene78362:CDS |
| GTGGTGTTAGGGTCGGAGAC+GGG | 0.487929 | 2.3:+4921907 | MS.gene78362:CDS |
| GGTGGTGTTAGGGTCGGAGA+CGG | 0.488468 | 2.3:+4921906 | MS.gene78362:CDS |
| TCATTTCATCGTCACTCATA+GGG | 0.491187 | 2.3:-4916258 | None:intergenic |
| CATTCCAAAACGAGCTGGAT+AGG | 0.497347 | 2.3:+4916146 | MS.gene78362:CDS |
| AAGATTTGGAGAGGCGAATG+TGG | 0.499638 | 2.3:+4915714 | MS.gene78362:CDS |
| TCTTGTGCTTGAGACTGCAT+TGG | 0.507375 | 2.3:-4915812 | None:intergenic |
| AAGAGAAGAGGTTGTGATTG+TGG | 0.508513 | 2.3:+4921885 | MS.gene78362:CDS |
| GTCGGAGACGGGTGTGAGAG+TGG | 0.520076 | 2.3:+4921918 | MS.gene78362:CDS |
| ACTTCCCCATCATCCAAAAC+CGG | 0.521167 | 2.3:-4918278 | None:intergenic |
| CTTACCTTATCACCCACAAA+TGG | 0.525327 | 2.3:-4918404 | None:intergenic |
| CCTAATTGGATCAATGTTCA+CGG | 0.526394 | 2.3:-4916032 | None:intergenic |
| CGCGAGCAAGAGGACGAAGA+AGG | 0.532184 | 2.3:+4921949 | MS.gene78362:CDS |
| TCAAATGACATGTTAGTGAT+TGG | 0.532346 | 2.3:-4916178 | None:intergenic |
| AACAATTGCATTTGTTCCCG+TGG | 0.534442 | 2.3:+4919995 | MS.gene78362:CDS |
| GATGAAATGATAAAGTACTT+TGG | 0.535924 | 2.3:+4916271 | MS.gene78362:CDS |
| AAGAAGAAGAGGAGGCGGTG+AGG | 0.536810 | 2.3:+4921838 | MS.gene78362:CDS |
| AAATGATAAAGTACTTTGGC+GGG | 0.539572 | 2.3:+4916275 | MS.gene78362:CDS |
| TCTAAAAGGAATGGCGGTGA+TGG | 0.540263 | 2.3:-4916223 | None:intergenic |
| TCGGAGACGGGTGTGAGAGT+GGG | 0.545633 | 2.3:+4921919 | MS.gene78362:CDS |
| CGGCGGTTGTGAAGAAGAAG+AGG | 0.547520 | 2.3:+4921827 | MS.gene78362:CDS |
| TGCTGCTGCCCCTCCTGACG+AGG | 0.548404 | 2.3:-4915986 | None:intergenic |
| TGATGGGGAAGTAGAGTATG+AGG | 0.548779 | 2.3:+4918289 | MS.gene78362:CDS |
| ACGAAGAAGGCTTGAGAATG+GGG | 0.549992 | 2.3:+4921962 | MS.gene78362:CDS |
| AAAAGGAATGGCGGTGATGG+TGG | 0.550954 | 2.3:-4916220 | None:intergenic |
| GAAATGATAAAGTACTTTGG+CGG | 0.553080 | 2.3:+4916274 | MS.gene78362:CDS |
| TTGGATCAATGTTCACGGCT+GGG | 0.555605 | 2.3:-4916027 | None:intergenic |
| CTTCATGTGGCATGTCAGGA+GGG | 0.560754 | 2.3:-4915925 | None:intergenic |
| GGGTCGGAGGCGCGAGCAAG+AGG | 0.561201 | 2.3:+4921939 | MS.gene78362:CDS |
| AGACGCATTCCAAAACGAGC+TGG | 0.561283 | 2.3:+4916141 | MS.gene78362:CDS |
| TTTGGAGAGGCGAATGTGGA+AGG | 0.562980 | 2.3:+4915718 | MS.gene78362:CDS |
| ATGAAAGGTGACGAAATCAC+TGG | 0.565348 | 2.3:+4916094 | MS.gene78362:CDS |
| GGCTGGTCCAGAATCTGCGT+GGG | 0.566158 | 2.3:+4918310 | MS.gene78362:CDS |
| CCAATTAGGCAGCCACAGAA+TGG | 0.567546 | 2.3:+4916046 | MS.gene78362:CDS |
| TGCAAGTGAGGGTAGTGCAA+TGG | 0.567879 | 2.3:-4915899 | None:intergenic |
| GATTGTGGTGGTGTTAGGGT+CGG | 0.568938 | 2.3:+4921900 | MS.gene78362:CDS |
| ATGAAATCATCTAAAAGGAA+TGG | 0.569614 | 2.3:-4916232 | None:intergenic |
| TTGAAGTTGAAGATTTGGAG+AGG | 0.571177 | 2.3:+4915705 | MS.gene78362:CDS |
| GGAATATGGTCCTCGTCAGG+AGG | 0.573065 | 2.3:+4915976 | MS.gene78362:CDS |
| GAATATGGTCCTCGTCAGGA+GGG | 0.581174 | 2.3:+4915977 | MS.gene78362:CDS |
| GCTTCATGTGGCATGTCAGG+AGG | 0.581231 | 2.3:-4915926 | None:intergenic |
| TTGTGAAGAAGAAGAGGAGG+CGG | 0.587466 | 2.3:+4921833 | MS.gene78362:CDS |
| AAAGGGAATTTACCTTGGGA+AGG | 0.587627 | 2.3:-4917959 | None:intergenic |
| TTTCGATCTTCACCTTCCCA+AGG | 0.592550 | 2.3:+4917947 | MS.gene78362:CDS |
| CGAAGAAGGCTTGAGAATGG+GGG | 0.596610 | 2.3:+4921963 | MS.gene78362:CDS |
| AGACGGGTGTGAGAGTGGGT+CGG | 0.597199 | 2.3:+4921923 | MS.gene78362:CDS |
| GGGGAAGTAGAGTATGAGGC+TGG | 0.604667 | 2.3:+4918293 | MS.gene78362:CDS |
| TAATCTTGACCTTAGAGTCG+CGG | 0.605222 | 2.3:+4915955 | MS.gene78362:CDS |
| GACAATGGTTGAATTACCAC+GGG | 0.609751 | 2.3:-4920011 | None:intergenic |
| CGGGTGTGAGAGTGGGTCGG+AGG | 0.614662 | 2.3:+4921926 | MS.gene78362:CDS |
| ATGCCTGTAGCAAATATGAA+AGG | 0.618350 | 2.3:+4916079 | MS.gene78362:CDS |
| TTGGAAAACATGTATGTGAG+TGG | 0.619040 | 2.3:+4915872 | MS.gene78362:CDS |
| AATATGGTCCTCGTCAGGAG+GGG | 0.626121 | 2.3:+4915978 | MS.gene78362:CDS |
| AGGCTGGTCCAGAATCTGCG+TGG | 0.639324 | 2.3:+4918309 | MS.gene78362:CDS |
| AGACAATGGTTGAATTACCA+CGG | 0.644788 | 2.3:-4920012 | None:intergenic |
| TATCAAACTCCAAAGGCTCA+AGG | 0.648346 | 2.3:+4915745 | MS.gene78362:CDS |
| AAAACATGTATGTGAGTGGA+AGG | 0.649522 | 2.3:+4915876 | MS.gene78362:CDS |
| AGATTATAAGCAGCTTCATG+TGG | 0.658467 | 2.3:-4915938 | None:intergenic |
| AAATCATCTAAAAGGAATGG+CGG | 0.661454 | 2.3:-4916229 | None:intergenic |
| ATAGTGATTGCACCATTCTG+TGG | 0.668976 | 2.3:-4916058 | None:intergenic |
| CGGTTGTGAAGAAGAAGAGG+AGG | 0.673596 | 2.3:+4921830 | MS.gene78362:CDS |
| AACGACGATATTGACGAGGA+CGG | 0.674455 | 2.3:+4921807 | MS.gene78362:CDS |
| AGGATCGTATCAAACTCCAA+AGG | 0.690382 | 2.3:+4915738 | MS.gene78362:CDS |
| AGAAGAGGTTGTGATTGTGG+TGG | 0.693045 | 2.3:+4921888 | MS.gene78362:CDS |
| AAAGGTGACGAAATCACTGG+AGG | 0.696560 | 2.3:+4916097 | MS.gene78362:CDS |
| GCTGGTCCAGAATCTGCGTG+GGG | 0.726249 | 2.3:+4918311 | MS.gene78362:CDS |
| GACGATATTGACGAGGACGG+CGG | 0.727647 | 2.3:+4921810 | MS.gene78362:CDS |
| AGTCAACGACGATATTGACG+AGG | 0.738502 | 2.3:+4921803 | MS.gene78362:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 4914898 | 4922004 | 4914898 | ID=MS.gene78362 |
| chr2.3 | mRNA | 4914898 | 4922004 | 4914898 | ID=MS.gene78362.t1;Parent=MS.gene78362 |
| chr2.3 | exon | 4914898 | 4914920 | 4914898 | ID=MS.gene78362.t1.exon1;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4914898 | 4914920 | 4914898 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| chr2.3 | exon | 4915664 | 4916296 | 4915664 | ID=MS.gene78362.t1.exon2;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4915664 | 4916296 | 4915664 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| chr2.3 | exon | 4917940 | 4917968 | 4917940 | ID=MS.gene78362.t1.exon3;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4917940 | 4917968 | 4917940 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| chr2.3 | exon | 4918258 | 4918421 | 4918258 | ID=MS.gene78362.t1.exon4;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4918258 | 4918421 | 4918258 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| chr2.3 | exon | 4919978 | 4920047 | 4919978 | ID=MS.gene78362.t1.exon5;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4919978 | 4920047 | 4919978 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| chr2.3 | exon | 4921739 | 4922004 | 4921739 | ID=MS.gene78362.t1.exon6;Parent=MS.gene78362.t1 |
| chr2.3 | CDS | 4921739 | 4922004 | 4921739 | ID=cds.MS.gene78362.t1;Parent=MS.gene78362.t1 |
| Gene Sequence |
| Protein sequence |
