AlfalfaGEDB | MS.gene79147

M. sativa cultivar XinJiangDaYe / MS.gene79147

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79147.t1	XP_004491126.1	70.5	88	20	3	16	99	102	187	1.30E-09	72.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79147.t1	A0A1S2XNN8	70.5	88	20	3	16	99	102	187	9.7e-10	72.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049701	MS.gene79147	0.829543	4.86E-55	-1.69E-46
MS.gene049853	MS.gene79147	0.8101	1.36E-50	-1.69E-46
MS.gene050611	MS.gene79147	0.800658	1.30E-48	-1.69E-46
MS.gene051710	MS.gene79147	0.818803	1.62E-52	-1.69E-46
MS.gene05404	MS.gene79147	0.837623	4.66E-57	-1.69E-46
MS.gene054174	MS.gene79147	0.839681	1.37E-57	-1.69E-46
MS.gene054175	MS.gene79147	0.845348	4.30E-59	-1.69E-46
MS.gene054484	MS.gene79147	0.83467	2.62E-56	-1.69E-46
MS.gene055056	MS.gene79147	0.831453	1.66E-55	-1.69E-46
MS.gene055058	MS.gene79147	0.809383	1.94E-50	-1.69E-46
MS.gene05762	MS.gene79147	0.812114	4.99E-51	-1.69E-46
MS.gene058515	MS.gene79147	0.849253	3.64E-60	-1.69E-46
MS.gene059276	MS.gene79147	0.833649	4.72E-56	-1.69E-46
MS.gene059584	MS.gene79147	0.805351	1.39E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79147.t1	MTR_5g085870	87.500	80	10	0	20	99	70	149	2.94e-30	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCGAACTCGTAAGGGGTTT+AGG	0.258839	7.2:-69483729	None:intergenic
TCGAACTCGTAAGGGGTTTA+GGG	0.305117	7.2:-69483728	None:intergenic
TTTCTTACCAATCGACCTTC+GGG	0.377700	7.2:-69483872	None:intergenic
TGTGACAGCGATTGCGAAAG+AGG	0.387116	7.2:-69483817	None:intergenic
AGAGAATCGGAAGGTGAAAC+AGG	0.391069	7.2:-69483766	None:intergenic
CTTCGATCCCGAAGGTCGAT+TGG	0.485685	7.2:+69483865	MS.gene79147:CDS
ATTGGTAAGAAAAGCGGCTC+TGG	0.495571	7.2:+69483883	MS.gene79147:CDS
AAAATCACGTGAAAGAGAAT+CGG	0.520635	7.2:-69483779	None:intergenic
AGAGAGAAATCGAACTCGTA+AGG	0.532543	7.2:-69483737	None:intergenic
GAGAGAAATCGAACTCGTAA+GGG	0.554082	7.2:-69483736	None:intergenic
AGAAAAGCGGCTCTGGTAGT+CGG	0.568712	7.2:+69483890	MS.gene79147:CDS
GCGAAAGAGGAGAGTCATCA+TGG	0.570240	7.2:-69483804	None:intergenic
GCTCTGGTAGTCGGCAAAGT+GGG	0.580582	7.2:+69483899	MS.gene79147:CDS
GGCTCTGGTAGTCGGCAAAG+TGG	0.629147	7.2:+69483898	MS.gene79147:CDS
ATTACGCTGGTGGTCAGAAG+AGG	0.632700	7.2:+69483989	MS.gene79147:CDS
AGAGAAATCGAACTCGTAAG+GGG	0.643399	7.2:-69483735	None:intergenic
AGGTCGATTGGTAAGAAAAG+CGG	0.647196	7.2:+69483877	MS.gene79147:CDS
TCGCAAGAGTATTACGCTGG+TGG	0.647638	7.2:+69483979	MS.gene79147:CDS
TGTATAAGCTTCGATCCCGA+AGG	0.650808	7.2:+69483857	MS.gene79147:CDS
GAATCGCAAGAGTATTACGC+TGG	0.665497	7.2:+69483976	MS.gene79147:CDS
TCACGTGAAAGAGAATCGGA+AGG	0.687567	7.2:-69483775	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAATCACGTGAAAGAGAAT+CGG	-	chr7.2:69483782-69483801	None:intergenic	30.0%
!	TTTTCTTACCAATCGACCTT+CGG	-	chr7.2:69483876-69483895	None:intergenic	35.0%
	AGAGAAATCGAACTCGTAAG+GGG	-	chr7.2:69483738-69483757	None:intergenic	40.0%
	AGAGAGAAATCGAACTCGTA+AGG	-	chr7.2:69483740-69483759	None:intergenic	40.0%
	AGGTCGATTGGTAAGAAAAG+CGG	+	chr7.2:69483877-69483896	MS.gene79147:CDS	40.0%
	GAGAGAAATCGAACTCGTAA+GGG	-	chr7.2:69483739-69483758	None:intergenic	40.0%
	TTTCTTACCAATCGACCTTC+GGG	-	chr7.2:69483875-69483894	None:intergenic	40.0%
	AGAGAATCGGAAGGTGAAAC+AGG	-	chr7.2:69483769-69483788	None:intergenic	45.0%
	ATCGAACTCGTAAGGGGTTT+AGG	-	chr7.2:69483732-69483751	None:intergenic	45.0%
	ATTGGTAAGAAAAGCGGCTC+TGG	+	chr7.2:69483883-69483902	MS.gene79147:CDS	45.0%
	TCACGTGAAAGAGAATCGGA+AGG	-	chr7.2:69483778-69483797	None:intergenic	45.0%
	TCGAACTCGTAAGGGGTTTA+GGG	-	chr7.2:69483731-69483750	None:intergenic	45.0%
	TGTATAAGCTTCGATCCCGA+AGG	+	chr7.2:69483857-69483876	MS.gene79147:CDS	45.0%
!!	GAATCGCAAGAGTATTACGC+TGG	+	chr7.2:69483976-69483995	MS.gene79147:CDS	45.0%
	ATTACGCTGGTGGTCAGAAG+AGG	+	chr7.2:69483989-69484008	MS.gene79147:CDS	50.0%
	GCGAAAGAGGAGAGTCATCA+TGG	-	chr7.2:69483807-69483826	None:intergenic	50.0%
	TGTGACAGCGATTGCGAAAG+AGG	-	chr7.2:69483820-69483839	None:intergenic	50.0%
!	AGAAAAGCGGCTCTGGTAGT+CGG	+	chr7.2:69483890-69483909	MS.gene79147:CDS	50.0%
!!	TCGCAAGAGTATTACGCTGG+TGG	+	chr7.2:69483979-69483998	MS.gene79147:CDS	50.0%
	CTTCGATCCCGAAGGTCGAT+TGG	+	chr7.2:69483865-69483884	MS.gene79147:CDS	55.0%
	GCTCTGGTAGTCGGCAAAGT+GGG	+	chr7.2:69483899-69483918	MS.gene79147:CDS	55.0%
!	GGCTCTGGTAGTCGGCAAAG+TGG	+	chr7.2:69483898-69483917	MS.gene79147:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	69483712	69484014	69483712	ID=MS.gene79147
chr7.2	mRNA	69483712	69484014	69483712	ID=MS.gene79147.t1;Parent=MS.gene79147
chr7.2	exon	69483712	69484014	69483712	ID=MS.gene79147.t1.exon1;Parent=MS.gene79147.t1
chr7.2	CDS	69483712	69484014	69483712	ID=cds.MS.gene79147.t1;Parent=MS.gene79147.t1

Gene Sequence	>MS.gene79147 ATGTCTCTCTTCAAATCCCTAAACCCCTTACGAGTTCGATTTCTCTCTTCAAATCCTGTTTCACCTTCCGATTCTCTTTCACGTGATTTTCACCATGATGACTCTCCTCTTTCGCAATCGCTGTCACAGTCGCTGTCACGTGCTCTGTATAAGCTTCGATCCCGAAGGTCGATTGGTAAGAAAAGCGGCTCTGGTAGTCGGCAAAGTGGGATTCAGACTTTGCGTGATGTGAAACGCTATCAAGATTCTGACTCTGAGTCTGATGAATCGCAAGAGTATTACGCTGGTGGTCAGAAGAGGTAA
Protein sequence	>MS.gene79147.t1 MSLFKSLNPLRVRFLSSNPVSPSDSLSRDFHHDDSPLSQSLSQSLSRALYKLRSRRSIGKKSGSGSRQSGIQTLRDVKRYQDSDSESDESQEYYAGGQKR