Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79571.t1 | XP_003621245.2 | 98.6 | 217 | 3 | 0 | 1 | 217 | 1 | 217 | 6.30E-113 | 416.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79571.t1 | P0C8Q9 | 54.1 | 98 | 43 | 1 | 120 | 217 | 150 | 245 | 1.7e-21 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79571.t1 | G7L200 | 98.6 | 217 | 3 | 0 | 1 | 217 | 1 | 217 | 4.5e-113 | 416.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene79571 | 0.830705 | 2.53E-55 | -1.69E-46 |
| MS.gene049419 | MS.gene79571 | -0.845491 | 3.93E-59 | -1.69E-46 |
| MS.gene049499 | MS.gene79571 | 0.831414 | 1.69E-55 | -1.69E-46 |
| MS.gene049983 | MS.gene79571 | 0.84026 | 9.68E-58 | -1.69E-46 |
| MS.gene050105 | MS.gene79571 | 0.812827 | 3.49E-51 | -1.69E-46 |
| MS.gene050312 | MS.gene79571 | 0.823441 | 1.38E-53 | -1.69E-46 |
| MS.gene051122 | MS.gene79571 | 0.827701 | 1.35E-54 | -1.69E-46 |
| MS.gene052674 | MS.gene79571 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene79571 | 0.801607 | 8.32E-49 | -1.69E-46 |
| MS.gene052954 | MS.gene79571 | 0.829192 | 5.91E-55 | -1.69E-46 |
| MS.gene053298 | MS.gene79571 | 0.801536 | 8.60E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene79571 | 0.819015 | 1.45E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene79571 | 0.825805 | 3.84E-54 | -1.69E-46 |
| MS.gene054479 | MS.gene79571 | 0.830361 | 3.07E-55 | -1.69E-46 |
| MS.gene054957 | MS.gene79571 | 0.808006 | 3.83E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene79571 | 0.804433 | 2.17E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene79571 | 0.82534 | 4.95E-54 | -1.69E-46 |
| MS.gene058008 | MS.gene79571 | 0.827769 | 1.30E-54 | -1.69E-46 |
| MS.gene058009 | MS.gene79571 | 0.810661 | 1.03E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene79571 | 0.802245 | 6.15E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene79571 | 0.840404 | 8.87E-58 | -1.69E-46 |
| MS.gene058223 | MS.gene79571 | -0.8219 | 3.16E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene79571 | 0.814721 | 1.33E-51 | -1.69E-46 |
| MS.gene058783 | MS.gene79571 | 0.818842 | 1.59E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene79571 | 0.843206 | 1.62E-58 | -1.69E-46 |
| MS.gene059952 | MS.gene79571 | 0.803059 | 4.18E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene79571 | 0.827988 | 1.15E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene79571 | 0.83423 | 3.38E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene79571 | 0.845723 | 3.40E-59 | -1.69E-46 |
| MS.gene06121 | MS.gene79571 | 0.813666 | 2.28E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79571.t1 | MTR_7g010930 | 98.618 | 217 | 3 | 0 | 1 | 217 | 1 | 217 | 4.73e-158 | 436 |
| MS.gene79571.t1 | MTR_6g012390 | 64.186 | 215 | 62 | 2 | 18 | 217 | 1 | 215 | 7.03e-95 | 276 |
| MS.gene79571.t1 | MTR_2g021720 | 42.574 | 101 | 46 | 2 | 120 | 217 | 103 | 194 | 6.41e-16 | 73.6 |
| MS.gene79571.t1 | MTR_2g021720 | 42.574 | 101 | 46 | 2 | 120 | 217 | 103 | 194 | 1.13e-15 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79571.t1 | AT3G01170 | 51.339 | 224 | 93 | 5 | 1 | 217 | 1 | 215 | 6.39e-67 | 205 |
| MS.gene79571.t1 | AT5G15260 | 51.681 | 238 | 90 | 8 | 1 | 217 | 1 | 234 | 9.25e-67 | 205 |
| MS.gene79571.t1 | AT5G15260 | 51.681 | 238 | 90 | 8 | 1 | 217 | 1 | 234 | 9.25e-67 | 205 |
| MS.gene79571.t1 | AT5G19025 | 54.082 | 98 | 43 | 1 | 120 | 217 | 150 | 245 | 8.09e-26 | 101 |
| MS.gene79571.t1 | AT5G19025 | 53.608 | 97 | 43 | 1 | 120 | 216 | 150 | 244 | 3.84e-25 | 99.4 |
| MS.gene79571.t1 | AT3G06180 | 53.061 | 98 | 44 | 1 | 120 | 217 | 132 | 227 | 6.51e-25 | 98.2 |
| MS.gene79571.t1 | AT3G01170 | 39.216 | 102 | 56 | 2 | 1 | 97 | 1 | 101 | 2.38e-11 | 60.1 |
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAGCTGTTGTTATTGCTTT+AGG | 0.138895 | 7.3:+87146664 | MS.gene79571:CDS |
| CAAACTTTGTGGTTTGTTTA+TGG | 0.153027 | 7.3:+87146732 | MS.gene79571:CDS |
| TTGATCTTTCTATTCTTCTT+TGG | 0.201359 | 7.3:-87147126 | None:intergenic |
| TGTTATTGCTTTAGGGTTCT+TGG | 0.201503 | 7.3:+87146672 | MS.gene79571:CDS |
| TAGCTGTTGTTATTGCTTTA+GGG | 0.217546 | 7.3:+87146665 | MS.gene79571:CDS |
| TTCTTCAATCCCTTACAATT+AGG | 0.224249 | 7.3:-87146877 | None:intergenic |
| GAATAGAAAGATCAAGAAAT+AGG | 0.263550 | 7.3:+87147134 | MS.gene79571:CDS |
| CTCATCACAAATTGAATATA+TGG | 0.264029 | 7.3:-87146700 | None:intergenic |
| TCTTCAATCCCTTACAATTA+GGG | 0.277107 | 7.3:-87146876 | None:intergenic |
| TGTTGGTAGGTTAGAAGTTC+CGG | 0.283392 | 7.3:+87147101 | MS.gene79571:CDS |
| AAGAAGATGGCTCCTGTTAA+TGG | 0.295596 | 7.3:+87147039 | MS.gene79571:CDS |
| AGATGGTGGTGGAATTAAAA+AGG | 0.303114 | 7.3:+87146966 | MS.gene79571:CDS |
| TTGTGGTTTGTTTATGGATT+TGG | 0.314324 | 7.3:+87146738 | MS.gene79571:CDS |
| CATGTGCTGTTGTTGCTACA+TGG | 0.391115 | 7.3:+87146818 | MS.gene79571:CDS |
| GTTGGTAGGTTAGAAGTTCC+GGG | 0.396075 | 7.3:+87147102 | MS.gene79571:CDS |
| AGTGAAAGTTTCAAACTTTG+TGG | 0.397466 | 7.3:+87146721 | MS.gene79571:CDS |
| CCTGTGATTTATGTATCTCT+TGG | 0.405703 | 7.3:+87146784 | MS.gene79571:CDS |
| AGAACAGCTCTTCCATTAAC+AGG | 0.409782 | 7.3:-87147051 | None:intergenic |
| ATGTGGGAACCCTAATTGTA+AGG | 0.424650 | 7.3:+87146867 | MS.gene79571:CDS |
| CGAATTCTTCACACAATCTT+CGG | 0.436293 | 7.3:-87146929 | None:intergenic |
| TGTGAAGAATTCGCCGTCGT+TGG | 0.440660 | 7.3:+87146939 | MS.gene79571:CDS |
| AGAGCTGTTCTAGTTCTTAG+AGG | 0.448409 | 7.3:+87147063 | MS.gene79571:CDS |
| GTGAAGAATTCGCCGTCGTT+GGG | 0.455176 | 7.3:+87146940 | MS.gene79571:CDS |
| AGTGTGGTTGTTCTGTTGGT+AGG | 0.472839 | 7.3:+87147088 | MS.gene79571:CDS |
| CAACGGATGAGATCAAACTA+TGG | 0.476169 | 7.3:-87146513 | None:intergenic |
| GGAAAGTGTGGTTGTTCTGT+TGG | 0.477218 | 7.3:+87147084 | MS.gene79571:CDS |
| GAAGAGAATTGAGTGTCTTA+CGG | 0.499754 | 7.3:-87146588 | None:intergenic |
| TCGATCCGCGGTTGTTGATG+TGG | 0.500037 | 7.3:+87146633 | MS.gene79571:CDS |
| CTTATACATCAAGGAAATGT+GGG | 0.514031 | 7.3:+87146851 | MS.gene79571:CDS |
| TTTCTATTCTTCTTTGGACC+CGG | 0.518411 | 7.3:-87147120 | None:intergenic |
| CGAAGCCGAGCTTAAGAAGA+TGG | 0.524432 | 7.3:+87147026 | MS.gene79571:CDS |
| AGGAGCCATCTTCTTAAGCT+CGG | 0.536872 | 7.3:-87147031 | None:intergenic |
| TCGCCGTCGTTGGGTAAAGA+TGG | 0.539719 | 7.3:+87146949 | MS.gene79571:CDS |
| GCTTATACATCAAGGAAATG+TGG | 0.544775 | 7.3:+87146850 | MS.gene79571:CDS |
| TGTGGGAACCCTAATTGTAA+GGG | 0.554639 | 7.3:+87146868 | MS.gene79571:CDS |
| TCGCCATGGATGATGATGAT+TGG | 0.560084 | 7.3:-87146537 | None:intergenic |
| CTAGTTCTTAGAGGAAAGTG+TGG | 0.579987 | 7.3:+87147072 | MS.gene79571:CDS |
| TCCTCGATTTAGCTAAATCG+AGG | 0.583685 | 7.3:-87146564 | None:intergenic |
| TGACCAATCATCATCATCCA+TGG | 0.587614 | 7.3:+87146534 | MS.gene79571:CDS |
| CCAAGAGATACATAAATCAC+AGG | 0.588766 | 7.3:-87146784 | None:intergenic |
| CCCTCGATTTAGCTAAATCG+AGG | 0.592975 | 7.3:+87146563 | MS.gene79571:CDS |
| GATCGAGATCGCTCGCACGA+CGG | 0.596853 | 7.3:-87146616 | None:intergenic |
| CCGTCGTTGGGTAAAGATGG+TGG | 0.619641 | 7.3:+87146952 | MS.gene79571:CDS |
| CCTCGATTTAGCTAAATCGA+GGG | 0.621163 | 7.3:-87146563 | None:intergenic |
| GTGCGAGCGATCTCGATCCG+CGG | 0.637914 | 7.3:+87146621 | MS.gene79571:CDS |
| TCGTTGGGTAAAGATGGTGG+TGG | 0.659209 | 7.3:+87146955 | MS.gene79571:CDS |
| AGTTCGCGATGATGATCACA+AGG | 0.663527 | 7.3:-87147003 | None:intergenic |
| GGATGATGATGATTGGTCAA+CGG | 0.679462 | 7.3:-87146530 | None:intergenic |
| TAAATCGAGGGAATTCGCCA+TGG | 0.679775 | 7.3:-87146551 | None:intergenic |
| CCACCATCTTTACCCAACGA+CGG | 0.694775 | 7.3:-87146952 | None:intergenic |
| TATAACCACATCAACAACCG+CGG | 0.698235 | 7.3:-87146638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATAGCTTATACATCA+AGG | + | chr7.3:87146842-87146861 | MS.gene79571:CDS | 20.0% |
| ! | CTCATCACAAATTGAATATA+TGG | - | chr7.3:87146703-87146722 | None:intergenic | 25.0% |
| ! | TTGATCTTTCTATTCTTCTT+TGG | - | chr7.3:87147129-87147148 | None:intergenic | 25.0% |
| AGTGAAAGTTTCAAACTTTG+TGG | + | chr7.3:87146721-87146740 | MS.gene79571:CDS | 30.0% | |
| CTTATACATCAAGGAAATGT+GGG | + | chr7.3:87146851-87146870 | MS.gene79571:CDS | 30.0% | |
| TCTTCAATCCCTTACAATTA+GGG | - | chr7.3:87146879-87146898 | None:intergenic | 30.0% | |
| TTCTTCAATCCCTTACAATT+AGG | - | chr7.3:87146880-87146899 | None:intergenic | 30.0% | |
| ! | ATAGCTGTTGTTATTGCTTT+AGG | + | chr7.3:87146664-87146683 | MS.gene79571:CDS | 30.0% |
| ! | TAGCTGTTGTTATTGCTTTA+GGG | + | chr7.3:87146665-87146684 | MS.gene79571:CDS | 30.0% |
| !! | CAAACTTTGTGGTTTGTTTA+TGG | + | chr7.3:87146732-87146751 | MS.gene79571:CDS | 30.0% |
| !! | TTGTGGTTTGTTTATGGATT+TGG | + | chr7.3:87146738-87146757 | MS.gene79571:CDS | 30.0% |
| CCAAGAGATACATAAATCAC+AGG | - | chr7.3:87146787-87146806 | None:intergenic | 35.0% | |
| CCTGTGATTTATGTATCTCT+TGG | + | chr7.3:87146784-87146803 | MS.gene79571:CDS | 35.0% | |
| CGAATTCTTCACACAATCTT+CGG | - | chr7.3:87146932-87146951 | None:intergenic | 35.0% | |
| GAAGAGAATTGAGTGTCTTA+CGG | - | chr7.3:87146591-87146610 | None:intergenic | 35.0% | |
| GCTTATACATCAAGGAAATG+TGG | + | chr7.3:87146850-87146869 | MS.gene79571:CDS | 35.0% | |
| TTTCTATTCTTCTTTGGACC+CGG | - | chr7.3:87147123-87147142 | None:intergenic | 35.0% | |
| !! | AGATGGTGGTGGAATTAAAA+AGG | + | chr7.3:87146966-87146985 | MS.gene79571:CDS | 35.0% |
| !! | GATGGTGGTGGAATTAAAAA+GGG | + | chr7.3:87146967-87146986 | MS.gene79571:CDS | 35.0% |
| !! | TGTTATTGCTTTAGGGTTCT+TGG | + | chr7.3:87146672-87146691 | MS.gene79571:CDS | 35.0% |
| AAGAAGATGGCTCCTGTTAA+TGG | + | chr7.3:87147039-87147058 | MS.gene79571:CDS | 40.0% | |
| AGAACAGCTCTTCCATTAAC+AGG | - | chr7.3:87147054-87147073 | None:intergenic | 40.0% | |
| ATGTGGGAACCCTAATTGTA+AGG | + | chr7.3:87146867-87146886 | MS.gene79571:CDS | 40.0% | |
| CAACGGATGAGATCAAACTA+TGG | - | chr7.3:87146516-87146535 | None:intergenic | 40.0% | |
| CCTCGATTTAGCTAAATCGA+GGG | - | chr7.3:87146583-87146564 | None:intergenic | 40.0% | |
| TATAACCACATCAACAACCG+CGG | - | chr7.3:87146641-87146660 | None:intergenic | 40.0% | |
| TCCTCGATTTAGCTAAATCG+AGG | - | chr7.3:87146567-87146586 | None:intergenic | 40.0% | |
| TGACCAATCATCATCATCCA+TGG | + | chr7.3:87146534-87146553 | MS.gene79571:CDS | 40.0% | |
| TGTGGGAACCCTAATTGTAA+GGG | + | chr7.3:87146868-87146887 | MS.gene79571:CDS | 40.0% | |
| ! | CTAGTTCTTAGAGGAAAGTG+TGG | + | chr7.3:87147072-87147091 | MS.gene79571:CDS | 40.0% |
| ! | GGATGATGATGATTGGTCAA+CGG | - | chr7.3:87146533-87146552 | None:intergenic | 40.0% |
| ! | TGTTGGTAGGTTAGAAGTTC+CGG | + | chr7.3:87147101-87147120 | MS.gene79571:CDS | 40.0% |
| !! | AGAGCTGTTCTAGTTCTTAG+AGG | + | chr7.3:87147063-87147082 | MS.gene79571:CDS | 40.0% |
| AGGAGCCATCTTCTTAAGCT+CGG | - | chr7.3:87147034-87147053 | None:intergenic | 45.0% | |
| AGTGTGGTTGTTCTGTTGGT+AGG | + | chr7.3:87147088-87147107 | MS.gene79571:CDS | 45.0% | |
| AGTTCGCGATGATGATCACA+AGG | - | chr7.3:87147006-87147025 | None:intergenic | 45.0% | |
| CCCTCGATTTAGCTAAATCG+AGG | + | chr7.3:87146563-87146582 | MS.gene79571:CDS | 45.0% | |
| TAAATCGAGGGAATTCGCCA+TGG | - | chr7.3:87146554-87146573 | None:intergenic | 45.0% | |
| ! | CATGTGCTGTTGTTGCTACA+TGG | + | chr7.3:87146818-87146837 | MS.gene79571:CDS | 45.0% |
| ! | GGAAAGTGTGGTTGTTCTGT+TGG | + | chr7.3:87147084-87147103 | MS.gene79571:CDS | 45.0% |
| ! | GTTGGTAGGTTAGAAGTTCC+GGG | + | chr7.3:87147102-87147121 | MS.gene79571:CDS | 45.0% |
| ! | TCGCCATGGATGATGATGAT+TGG | - | chr7.3:87146540-87146559 | None:intergenic | 45.0% |
| CCACCATCTTTACCCAACGA+CGG | - | chr7.3:87146955-87146974 | None:intergenic | 50.0% | |
| CGAAGCCGAGCTTAAGAAGA+TGG | + | chr7.3:87147026-87147045 | MS.gene79571:CDS | 50.0% | |
| GTGAAGAATTCGCCGTCGTT+GGG | + | chr7.3:87146940-87146959 | MS.gene79571:CDS | 50.0% | |
| TGTGAAGAATTCGCCGTCGT+TGG | + | chr7.3:87146939-87146958 | MS.gene79571:CDS | 50.0% | |
| ! | TCGTTGGGTAAAGATGGTGG+TGG | + | chr7.3:87146955-87146974 | MS.gene79571:CDS | 50.0% |
| CCGTCGTTGGGTAAAGATGG+TGG | + | chr7.3:87146952-87146971 | MS.gene79571:CDS | 55.0% | |
| TCGATCCGCGGTTGTTGATG+TGG | + | chr7.3:87146633-87146652 | MS.gene79571:CDS | 55.0% | |
| TCGCCGTCGTTGGGTAAAGA+TGG | + | chr7.3:87146949-87146968 | MS.gene79571:CDS | 55.0% | |
| GATCGAGATCGCTCGCACGA+CGG | - | chr7.3:87146619-87146638 | None:intergenic | 60.0% | |
| GTGCGAGCGATCTCGATCCG+CGG | + | chr7.3:87146621-87146640 | MS.gene79571:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 87146502 | 87147155 | 87146502 | ID=MS.gene79571 |
| chr7.3 | mRNA | 87146502 | 87147155 | 87146502 | ID=MS.gene79571.t1;Parent=MS.gene79571 |
| chr7.3 | exon | 87146502 | 87147155 | 87146502 | ID=MS.gene79571.t1.exon1;Parent=MS.gene79571.t1 |
| chr7.3 | CDS | 87146502 | 87147155 | 87146502 | ID=cds.MS.gene79571.t1;Parent=MS.gene79571.t1 |
| Gene Sequence |
| Protein sequence |
