Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82354.t1 | GAU32879.1 | 75.6 | 86 | 21 | 0 | 1 | 86 | 1 | 86 | 2.00E-25 | 124.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82354.t1 | A0A2Z6NMK5 | 75.6 | 86 | 21 | 0 | 1 | 86 | 1 | 86 | 1.4e-25 | 124.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049438 | MS.gene82354 | 0.828202 | 1.03E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene82354 | -0.802342 | 5.87E-49 | -1.69E-46 |
| MS.gene051452 | MS.gene82354 | 0.817156 | 3.82E-52 | -1.69E-46 |
| MS.gene051655 | MS.gene82354 | 0.805063 | 1.60E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene82354 | 0.800312 | 1.53E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene82354 | -0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene053298 | MS.gene82354 | 0.816821 | 4.54E-52 | -1.69E-46 |
| MS.gene053340 | MS.gene82354 | 0.805178 | 1.51E-49 | -1.69E-46 |
| MS.gene05344 | MS.gene82354 | -0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene055063 | MS.gene82354 | -0.822269 | 2.59E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene82354 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene82354 | 0.845123 | 4.94E-59 | -1.69E-46 |
| MS.gene057452 | MS.gene82354 | -0.8206 | 6.30E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene82354 | 0.816395 | 5.66E-52 | -1.69E-46 |
| MS.gene059830 | MS.gene82354 | 0.814665 | 1.37E-51 | -1.69E-46 |
| MS.gene059872 | MS.gene82354 | -0.851006 | 1.18E-60 | -1.69E-46 |
| MS.gene060297 | MS.gene82354 | 0.838853 | 2.25E-57 | -1.69E-46 |
| MS.gene060869 | MS.gene82354 | 0.834861 | 2.35E-56 | -1.69E-46 |
| MS.gene060870 | MS.gene82354 | 0.805073 | 1.59E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene82354 | 0.815025 | 1.14E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82354.t1 | MTR_4g082883 | 49.333 | 75 | 33 | 2 | 16 | 86 | 26 | 99 | 1.42e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGTAGAAACTTATT+TGG | 0.193374 | 4.2:+26786889 | None:intergenic |
| GTTTCTACCAAGTGAGGTTT+TGG | 0.234806 | 4.2:-26786881 | MS.gene82354:CDS |
| AGCACACCACCTTCGGATTT+AGG | 0.251846 | 4.2:+26786709 | None:intergenic |
| GAGAAGGTGTCAACTGCTGC+AGG | 0.400905 | 4.2:-26786805 | MS.gene82354:CDS |
| TCCGAAGGTGGTGTGCTTGA+TGG | 0.441201 | 4.2:-26786703 | MS.gene82354:CDS |
| ACCATCAAGCACACCACCTT+CGG | 0.487811 | 4.2:+26786702 | None:intergenic |
| CTGTAGTTGAGCATGTTGAC+AGG | 0.490478 | 4.2:-26786768 | MS.gene82354:CDS |
| TCGGATTTAGGAGGCTCCTC+TGG | 0.493494 | 4.2:+26786721 | None:intergenic |
| GAGTCAGCAGAGACAACTGC+AGG | 0.516751 | 4.2:-26786838 | MS.gene82354:CDS |
| GCACTCCCCAAAACCTCACT+TGG | 0.541506 | 4.2:+26786874 | None:intergenic |
| AAAGAAACAATGGCAGAAAG+TGG | 0.543749 | 4.2:-26786916 | None:intergenic |
| AAATAAGTTTCTACCAAGTG+AGG | 0.567992 | 4.2:-26786887 | MS.gene82354:CDS |
| GGTGTCAACTGCTGCAGGGG+AGG | 0.571411 | 4.2:-26786800 | MS.gene82354:CDS |
| TTTAGGAGGCTCCTCTGGTG+TGG | 0.573361 | 4.2:+26786726 | None:intergenic |
| AGAAGGTGTCAACTGCTGCA+GGG | 0.573757 | 4.2:-26786804 | MS.gene82354:CDS |
| GAAGGTGTCAACTGCTGCAG+GGG | 0.582580 | 4.2:-26786803 | MS.gene82354:CDS |
| ACACCACCTTCGGATTTAGG+AGG | 0.610944 | 4.2:+26786712 | None:intergenic |
| TGCAGGGAAACAATCTGAGA+AGG | 0.618025 | 4.2:-26786821 | MS.gene82354:CDS |
| GAGGAGCCTCCTAAATCCGA+AGG | 0.645422 | 4.2:-26786718 | MS.gene82354:CDS |
| TCATGTTCATTCCACACCAG+AGG | 0.674914 | 4.2:-26786737 | MS.gene82354:CDS |
| GAGCCTCCTAAATCCGAAGG+TGG | 0.678300 | 4.2:-26786715 | MS.gene82354:CDS |
| AGTCAGCAGAGACAACTGCA+GGG | 0.685909 | 4.2:-26786837 | MS.gene82354:CDS |
| TGTAGTTGAGCATGTTGACA+GGG | 0.688044 | 4.2:-26786767 | MS.gene82354:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGTTTCTACCAAGTG+AGG | - | chr4.2:26786689-26786708 | MS.gene82354:CDS | 30.0% | |
| TCACTTGGTAGAAACTTATT+TGG | + | chr4.2:26786690-26786709 | None:intergenic | 30.0% | |
| ! | GATGGTGTTAAAAAAGCTGT+TGG | - | chr4.2:26786891-26786910 | MS.gene82354:CDS | 35.0% |
| ! | TTTCTACCAAGTGAGGTTTT+GGG | - | chr4.2:26786696-26786715 | MS.gene82354:CDS | 35.0% |
| GGTGTTAAAAAAGCTGTTGG+AGG | - | chr4.2:26786894-26786913 | MS.gene82354:CDS | 40.0% | |
| TGTAGTTGAGCATGTTGACA+GGG | - | chr4.2:26786809-26786828 | MS.gene82354:CDS | 40.0% | |
| ! | GTTTCTACCAAGTGAGGTTT+TGG | - | chr4.2:26786695-26786714 | MS.gene82354:CDS | 40.0% |
| ! | TTCTACCAAGTGAGGTTTTG+GGG | - | chr4.2:26786697-26786716 | MS.gene82354:CDS | 40.0% |
| CTGTAGTTGAGCATGTTGAC+AGG | - | chr4.2:26786808-26786827 | MS.gene82354:CDS | 45.0% | |
| TCATGTTCATTCCACACCAG+AGG | - | chr4.2:26786839-26786858 | MS.gene82354:CDS | 45.0% | |
| TGCAGGGAAACAATCTGAGA+AGG | - | chr4.2:26786755-26786774 | MS.gene82354:CDS | 45.0% | |
| ACACCACCTTCGGATTTAGG+AGG | + | chr4.2:26786867-26786886 | None:intergenic | 50.0% | |
| ACCATCAAGCACACCACCTT+CGG | + | chr4.2:26786877-26786896 | None:intergenic | 50.0% | |
| AGCACACCACCTTCGGATTT+AGG | + | chr4.2:26786870-26786889 | None:intergenic | 50.0% | |
| AGTCAGCAGAGACAACTGCA+GGG | - | chr4.2:26786739-26786758 | MS.gene82354:CDS | 50.0% | |
| !! | AGAAGGTGTCAACTGCTGCA+GGG | - | chr4.2:26786772-26786791 | MS.gene82354:CDS | 50.0% |
| !!! | GTTTTGGGGAGTGCAAAGGT+GGG | - | chr4.2:26786711-26786730 | MS.gene82354:CDS | 50.0% |
| !!! | TGAGGTTTTGGGGAGTGCAA+AGG | - | chr4.2:26786707-26786726 | MS.gene82354:CDS | 50.0% |
| GAGCCTCCTAAATCCGAAGG+TGG | - | chr4.2:26786861-26786880 | MS.gene82354:CDS | 55.0% | |
| GAGGAGCCTCCTAAATCCGA+AGG | - | chr4.2:26786858-26786877 | MS.gene82354:CDS | 55.0% | |
| GAGTCAGCAGAGACAACTGC+AGG | - | chr4.2:26786738-26786757 | MS.gene82354:CDS | 55.0% | |
| GCACTCCCCAAAACCTCACT+TGG | + | chr4.2:26786705-26786724 | None:intergenic | 55.0% | |
| TCCGAAGGTGGTGTGCTTGA+TGG | - | chr4.2:26786873-26786892 | MS.gene82354:CDS | 55.0% | |
| TCGGATTTAGGAGGCTCCTC+TGG | + | chr4.2:26786858-26786877 | None:intergenic | 55.0% | |
| TTTAGGAGGCTCCTCTGGTG+TGG | + | chr4.2:26786853-26786872 | None:intergenic | 55.0% | |
| !! | GAAGGTGTCAACTGCTGCAG+GGG | - | chr4.2:26786773-26786792 | MS.gene82354:CDS | 55.0% |
| !! | GAGAAGGTGTCAACTGCTGC+AGG | - | chr4.2:26786771-26786790 | MS.gene82354:CDS | 55.0% |
| !!! | GGTTTTGGGGAGTGCAAAGG+TGG | - | chr4.2:26786710-26786729 | MS.gene82354:CDS | 55.0% |
| GGTGTCAACTGCTGCAGGGG+AGG | - | chr4.2:26786776-26786795 | MS.gene82354:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26786669 | 26786929 | 26786669 | ID=MS.gene82354 |
| chr4.2 | mRNA | 26786669 | 26786929 | 26786669 | ID=MS.gene82354.t1;Parent=MS.gene82354 |
| chr4.2 | exon | 26786669 | 26786929 | 26786669 | ID=MS.gene82354.t1.exon1;Parent=MS.gene82354.t1 |
| chr4.2 | CDS | 26786669 | 26786929 | 26786669 | ID=cds.MS.gene82354.t1;Parent=MS.gene82354.t1 |
| Gene Sequence |
| Protein sequence |
