Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82668.t1 | RHN67051.1 | 84.4 | 160 | 25 | 0 | 1 | 160 | 1 | 160 | 1.30E-69 | 272.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82668.t1 | Q9FKI4 | 37.4 | 115 | 69 | 3 | 39 | 151 | 42 | 155 | 9.2e-08 | 58.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82668.t1 | A0A396IQQ6 | 84.4 | 160 | 25 | 0 | 1 | 160 | 1 | 160 | 9.3e-70 | 272.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82668.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049587 | MS.gene82668 | 0.817483 | 3.22E-52 | -1.69E-46 |
| MS.gene049853 | MS.gene82668 | 0.865096 | 7.57E-65 | -1.69E-46 |
| MS.gene050611 | MS.gene82668 | 0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene052447 | MS.gene82668 | 0.808634 | 2.81E-50 | -1.69E-46 |
| MS.gene054174 | MS.gene82668 | 0.819725 | 9.99E-53 | -1.69E-46 |
| MS.gene054175 | MS.gene82668 | 0.822224 | 2.66E-53 | -1.69E-46 |
| MS.gene054484 | MS.gene82668 | 0.849769 | 2.61E-60 | -1.69E-46 |
| MS.gene055017 | MS.gene82668 | 0.826956 | 2.04E-54 | -1.69E-46 |
| MS.gene055056 | MS.gene82668 | 0.803041 | 4.21E-49 | -1.69E-46 |
| MS.gene055058 | MS.gene82668 | 0.819638 | 1.05E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene82668 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene82668 | 0.835324 | 1.79E-56 | -1.69E-46 |
| MS.gene058580 | MS.gene82668 | 0.806625 | 7.51E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene82668 | 0.847203 | 1.34E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82668.t1 | MTR_3g050860 | 84.906 | 159 | 24 | 0 | 1 | 159 | 1 | 159 | 6.44e-95 | 280 |
| MS.gene82668.t1 | MTR_0439s0020 | 74.825 | 143 | 35 | 1 | 1 | 142 | 1 | 143 | 1.99e-73 | 218 |
| MS.gene82668.t1 | MTR_3g030055 | 34.395 | 157 | 87 | 7 | 1 | 152 | 8 | 153 | 3.93e-14 | 67.0 |
| MS.gene82668.t1 | MTR_3g034200 | 33.117 | 154 | 93 | 4 | 6 | 150 | 2 | 154 | 4.67e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82668.t1 | AT5G13780 | 37.838 | 111 | 66 | 3 | 43 | 151 | 46 | 155 | 3.80e-11 | 59.7 |
Find 55 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTGAAGTTATTACATCTT+AGG | 0.242413 | 3.1:+37394484 | MS.gene82668:CDS |
| AATGCGTCAGTGTTGGTCTT+TGG | 0.291887 | 3.1:-37394054 | None:intergenic |
| TGTTGACCTATATAAGATTC+TGG | 0.336459 | 3.1:+37393601 | MS.gene82668:CDS |
| TTCTACAACGATGGTGTCCC+CGG | 0.363342 | 3.1:+37393553 | None:intergenic |
| ACTTGTGACCATGGCTGAAA+TGG | 0.368546 | 3.1:+37393820 | MS.gene82668:CDS |
| ATTACTCTTCTTCGAAAAGA+TGG | 0.379614 | 3.1:+37394074 | MS.gene82668:CDS |
| ATGCGTCAGTGTTGGTCTTT+GGG | 0.387142 | 3.1:-37394053 | None:intergenic |
| TCACTTCACGAGGCTCGTTC+AGG | 0.391717 | 3.1:-37394467 | None:intergenic |
| TGACGTGGATACTCAAAGTT+TGG | 0.414598 | 3.1:+37393640 | MS.gene82668:CDS |
| GGCCCTATCTTTCGAAGAAA+AGG | 0.417511 | 3.1:+37393785 | MS.gene82668:CDS |
| GTGAAGAGCAATAGTATTGA+TGG | 0.420089 | 3.1:+37394114 | MS.gene82668:CDS |
| AACGATGGTGTCCCCGGGTG+TGG | 0.437378 | 3.1:+37393559 | None:intergenic |
| AGAATCTTATATAGGTCAAC+AGG | 0.439517 | 3.1:-37393599 | None:intergenic |
| AGTGAAGTTATTACATCTTA+GGG | 0.441387 | 3.1:+37394485 | MS.gene82668:CDS |
| GGCTGAAATGGACTTGGAAA+AGG | 0.444198 | 3.1:+37393832 | MS.gene82668:CDS |
| CATATCACTTCCCTCTTCGT+TGG | 0.453367 | 3.1:+37393764 | MS.gene82668:CDS |
| GAACTGAAGCGTACTGGTTA+CGG | 0.472216 | 3.1:+37393740 | MS.gene82668:CDS |
| CTTCGAAAAGATGGGTTATG+AGG | 0.474734 | 3.1:+37394083 | MS.gene82668:CDS |
| GGTTACCTTGTCTTTGATGT+TGG | 0.476676 | 3.1:+37393716 | MS.gene82668:CDS |
| TCTAATACCTCATCTTCATC+AGG | 0.477800 | 3.1:-37394513 | None:intergenic |
| GTCAGTGTTGGTCTTTGGGA+TGG | 0.506964 | 3.1:-37394049 | None:intergenic |
| TTACTCTTCTTCGAAAAGAT+GGG | 0.507362 | 3.1:+37394075 | MS.gene82668:CDS |
| TTCCAAGTCCATTTCAGCCA+TGG | 0.509729 | 3.1:-37393828 | None:intergenic |
| GACCATGGCTGAAATGGACT+TGG | 0.509783 | 3.1:+37393826 | MS.gene82668:CDS |
| CATTCACCAGAATCTTATAT+AGG | 0.510974 | 3.1:-37393607 | None:intergenic |
| GCGTCTCTTACGACCACACC+CGG | 0.518110 | 3.1:-37393572 | None:intergenic |
| GATACTCAAAGTTTGGTGAA+TGG | 0.534335 | 3.1:+37393647 | MS.gene82668:CDS |
| TCAGTGTTGGTCTTTGGGAT+GGG | 0.537407 | 3.1:-37394048 | None:intergenic |
| CGCTACTCGACTTGTGACCA+TGG | 0.544412 | 3.1:+37393811 | MS.gene82668:CDS |
| ATCATGACGAAGAAGCTCAA+GGG | 0.553264 | 3.1:+37394156 | MS.gene82668:CDS |
| AAACTTTGAGTATCCACGTC+AGG | 0.555864 | 3.1:-37393638 | None:intergenic |
| GCAAGGTGCTGCTTACGTCT+CGG | 0.567387 | 3.1:+37394023 | MS.gene82668:intron |
| TCTACAACGATGGTGTCCCC+GGG | 0.568555 | 3.1:+37393554 | None:intergenic |
| GTTGCAGCAGATGATCTCCT+AGG | 0.569948 | 3.1:+37393683 | MS.gene82668:CDS |
| AAAGATGGGTTATGAGGAGG+AGG | 0.574952 | 3.1:+37394089 | MS.gene82668:CDS |
| GATGGGTTATGAGGAGGAGG+CGG | 0.578268 | 3.1:+37394092 | MS.gene82668:CDS |
| GTTGGTGAACTGAAGCGTAC+TGG | 0.578758 | 3.1:+37393734 | MS.gene82668:CDS |
| AAAGATAGGGCCAACGAAGA+GGG | 0.584375 | 3.1:-37393774 | None:intergenic |
| ATGCGTTCTGCGGAGACCGA+AGG | 0.587376 | 3.1:+37394186 | MS.gene82668:CDS |
| GAAGAGTAATGCGTCAGTGT+TGG | 0.587807 | 3.1:-37394061 | None:intergenic |
| TGAAGAGCAATAGTATTGAT+GGG | 0.595651 | 3.1:+37394115 | MS.gene82668:CDS |
| GAAAGATAGGGCCAACGAAG+AGG | 0.603202 | 3.1:-37393775 | None:intergenic |
| AATCATGACGAAGAAGCTCA+AGG | 0.613048 | 3.1:+37394155 | MS.gene82668:CDS |
| CGAAAAGATGGGTTATGAGG+AGG | 0.622267 | 3.1:+37394086 | MS.gene82668:CDS |
| GGTAACCGAGGATAATGCCT+AGG | 0.623647 | 3.1:-37393700 | None:intergenic |
| TCATGACGAAGAAGCTCAAG+GGG | 0.629217 | 3.1:+37394157 | MS.gene82668:CDS |
| CGTCTCTTACGACCACACCC+GGG | 0.631420 | 3.1:-37393571 | None:intergenic |
| GGTCTGTCCTGATGAAGATG+AGG | 0.638915 | 3.1:+37394506 | MS.gene82668:CDS |
| GATCTCCTAGGCATTATCCT+CGG | 0.649171 | 3.1:+37393695 | MS.gene82668:CDS |
| GTCTCTTACGACCACACCCG+GGG | 0.653034 | 3.1:-37393570 | None:intergenic |
| TGTAATAACTTCACTTCACG+AGG | 0.654474 | 3.1:-37394477 | None:intergenic |
| CATCAAAGACAAGGTAACCG+AGG | 0.661196 | 3.1:-37393712 | None:intergenic |
| GGGGAAGCAGATGCGTTCTG+CGG | 0.668612 | 3.1:+37394176 | MS.gene82668:CDS |
| GTTCACCAACATCAAAGACA+AGG | 0.681557 | 3.1:-37393721 | None:intergenic |
| GAATGATGTCTTTCCTGACG+TGG | 0.685326 | 3.1:+37393625 | MS.gene82668:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTAAAATCATATATGTT+AGG | + | chr3.1:37393911-37393930 | MS.gene82668:intron | 15.0% |
| !! | CTATTCATGATATTAAAGTT+TGG | + | chr3.1:37394420-37394439 | MS.gene82668:intron | 20.0% |
| ! | AAAATAAACAGCCAAAAAAG+TGG | - | chr3.1:37394248-37394267 | None:intergenic | 25.0% |
| ! | AAATAAACAGCCAAAAAAGT+GGG | - | chr3.1:37394247-37394266 | None:intergenic | 25.0% |
| ! | AAGTGAAGTTATTACATCTT+AGG | + | chr3.1:37394484-37394503 | MS.gene82668:CDS | 25.0% |
| ! | AGTGAAGTTATTACATCTTA+GGG | + | chr3.1:37394485-37394504 | MS.gene82668:CDS | 25.0% |
| ! | GAAAACAATTGCAACTTTAT+TGG | + | chr3.1:37394283-37394302 | MS.gene82668:intron | 25.0% |
| AGAATCTTATATAGGTCAAC+AGG | - | chr3.1:37393602-37393621 | None:intergenic | 30.0% | |
| ATTACTCTTCTTCGAAAAGA+TGG | + | chr3.1:37394074-37394093 | MS.gene82668:CDS | 30.0% | |
| CATTCACCAGAATCTTATAT+AGG | - | chr3.1:37393610-37393629 | None:intergenic | 30.0% | |
| TGAATAATGATGTGAGTGAA+AGG | + | chr3.1:37394371-37394390 | MS.gene82668:intron | 30.0% | |
| TGTTGACCTATATAAGATTC+TGG | + | chr3.1:37393601-37393620 | MS.gene82668:CDS | 30.0% | |
| TTACTCTTCTTCGAAAAGAT+GGG | + | chr3.1:37394075-37394094 | MS.gene82668:CDS | 30.0% | |
| ! | TTGGTGCTTATTACATCTTA+GGG | + | chr3.1:37394439-37394458 | MS.gene82668:intron | 30.0% |
| !! | TGAAGAGCAATAGTATTGAT+GGG | + | chr3.1:37394115-37394134 | MS.gene82668:CDS | 30.0% |
| !! | TGGAAAAGGTTTTGTATGTT+TGG | + | chr3.1:37393846-37393865 | MS.gene82668:intron | 30.0% |
| !! | TTTGGTGCTTATTACATCTT+AGG | + | chr3.1:37394438-37394457 | MS.gene82668:intron | 30.0% |
| TCTAATACCTCATCTTCATC+AGG | - | chr3.1:37394516-37394535 | None:intergenic | 35.0% | |
| TGTAATAACTTCACTTCACG+AGG | - | chr3.1:37394480-37394499 | None:intergenic | 35.0% | |
| ! | ACAAGCGTGTCTTCTTTTAA+TGG | + | chr3.1:37393954-37393973 | MS.gene82668:intron | 35.0% |
| ! | GATACTCAAAGTTTGGTGAA+TGG | + | chr3.1:37393647-37393666 | MS.gene82668:CDS | 35.0% |
| ! | GGTTGGTGTAGTCTTTAATA+TGG | + | chr3.1:37393980-37393999 | MS.gene82668:intron | 35.0% |
| ! | GTCCTTTTCTTCGAAAGATA+GGG | - | chr3.1:37393790-37393809 | None:intergenic | 35.0% |
| ! | GTGAAGAGCAATAGTATTGA+TGG | + | chr3.1:37394114-37394133 | MS.gene82668:CDS | 35.0% |
| ! | TGTCCTTTTCTTCGAAAGAT+AGG | - | chr3.1:37393791-37393810 | None:intergenic | 35.0% |
| AAACTTTGAGTATCCACGTC+AGG | - | chr3.1:37393641-37393660 | None:intergenic | 40.0% | |
| AATCATGACGAAGAAGCTCA+AGG | + | chr3.1:37394155-37394174 | MS.gene82668:CDS | 40.0% | |
| AGCCAAAAAAGTGGGCAAAT+CGG | - | chr3.1:37394239-37394258 | None:intergenic | 40.0% | |
| ATCATGACGAAGAAGCTCAA+GGG | + | chr3.1:37394156-37394175 | MS.gene82668:CDS | 40.0% | |
| CTGTTACTGTGTTTGTAGCA+AGG | + | chr3.1:37394006-37394025 | MS.gene82668:intron | 40.0% | |
| GGTTACCTTGTCTTTGATGT+TGG | + | chr3.1:37393716-37393735 | MS.gene82668:CDS | 40.0% | |
| GTTCACCAACATCAAAGACA+AGG | - | chr3.1:37393724-37393743 | None:intergenic | 40.0% | |
| TGACGTGGATACTCAAAGTT+TGG | + | chr3.1:37393640-37393659 | MS.gene82668:CDS | 40.0% | |
| ! | AGTGCCTTTTGCATTACCTT+CGG | - | chr3.1:37394205-37394224 | None:intergenic | 40.0% |
| !! | CTTCGAAAAGATGGGTTATG+AGG | + | chr3.1:37394083-37394102 | MS.gene82668:CDS | 40.0% |
| AAAGATAGGGCCAACGAAGA+GGG | - | chr3.1:37393777-37393796 | None:intergenic | 45.0% | |
| ACTTGTGACCATGGCTGAAA+TGG | + | chr3.1:37393820-37393839 | MS.gene82668:CDS | 45.0% | |
| CATATCACTTCCCTCTTCGT+TGG | + | chr3.1:37393764-37393783 | MS.gene82668:CDS | 45.0% | |
| CATCAAAGACAAGGTAACCG+AGG | - | chr3.1:37393715-37393734 | None:intergenic | 45.0% | |
| GAAGAGTAATGCGTCAGTGT+TGG | - | chr3.1:37394064-37394083 | None:intergenic | 45.0% | |
| GAATGATGTCTTTCCTGACG+TGG | + | chr3.1:37393625-37393644 | MS.gene82668:CDS | 45.0% | |
| GAGACCGAAGGTAATGCAAA+AGG | + | chr3.1:37394198-37394217 | MS.gene82668:intron | 45.0% | |
| GATCTCCTAGGCATTATCCT+CGG | + | chr3.1:37393695-37393714 | MS.gene82668:CDS | 45.0% | |
| GGCCCTATCTTTCGAAGAAA+AGG | + | chr3.1:37393785-37393804 | MS.gene82668:CDS | 45.0% | |
| TCATGACGAAGAAGCTCAAG+GGG | + | chr3.1:37394157-37394176 | MS.gene82668:CDS | 45.0% | |
| TTCCAAGTCCATTTCAGCCA+TGG | - | chr3.1:37393831-37393850 | None:intergenic | 45.0% | |
| ! | CGTGTCTTCTTTTAATGGCG+CGG | + | chr3.1:37393959-37393978 | MS.gene82668:intron | 45.0% |
| ! | GGCTGAAATGGACTTGGAAA+AGG | + | chr3.1:37393832-37393851 | MS.gene82668:CDS | 45.0% |
| ! | TCTTCTTTTAATGGCGCGGT+TGG | + | chr3.1:37393963-37393982 | MS.gene82668:intron | 45.0% |
| !! | AAAGATGGGTTATGAGGAGG+AGG | + | chr3.1:37394089-37394108 | MS.gene82668:CDS | 45.0% |
| !! | AATGCGTCAGTGTTGGTCTT+TGG | - | chr3.1:37394057-37394076 | None:intergenic | 45.0% |
| !! | ATGCGTCAGTGTTGGTCTTT+GGG | - | chr3.1:37394056-37394075 | None:intergenic | 45.0% |
| !! | CGAAAAGATGGGTTATGAGG+AGG | + | chr3.1:37394086-37394105 | MS.gene82668:CDS | 45.0% |
| !! | GAACTGAAGCGTACTGGTTA+CGG | + | chr3.1:37393740-37393759 | MS.gene82668:CDS | 45.0% |
| !! | TCAGTGTTGGTCTTTGGGAT+GGG | - | chr3.1:37394051-37394070 | None:intergenic | 45.0% |
| !!! | GACCGATTTGCCCACTTTTT+TGG | + | chr3.1:37394234-37394253 | MS.gene82668:intron | 45.0% |
| GAAAGATAGGGCCAACGAAG+AGG | - | chr3.1:37393778-37393797 | None:intergenic | 50.0% | |
| GGTAACCGAGGATAATGCCT+AGG | - | chr3.1:37393703-37393722 | None:intergenic | 50.0% | |
| GGTCTGTCCTGATGAAGATG+AGG | + | chr3.1:37394506-37394525 | MS.gene82668:CDS | 50.0% | |
| GTTGCAGCAGATGATCTCCT+AGG | + | chr3.1:37393683-37393702 | MS.gene82668:CDS | 50.0% | |
| ! | GACCATGGCTGAAATGGACT+TGG | + | chr3.1:37393826-37393845 | MS.gene82668:CDS | 50.0% |
| ! | GTTGGTGAACTGAAGCGTAC+TGG | + | chr3.1:37393734-37393753 | MS.gene82668:CDS | 50.0% |
| !! | GTCAGTGTTGGTCTTTGGGA+TGG | - | chr3.1:37394052-37394071 | None:intergenic | 50.0% |
| TCACTTCACGAGGCTCGTTC+AGG | - | chr3.1:37394470-37394489 | None:intergenic | 55.0% | |
| ! | CGCTACTCGACTTGTGACCA+TGG | + | chr3.1:37393811-37393830 | MS.gene82668:CDS | 55.0% |
| !! | GATGGGTTATGAGGAGGAGG+CGG | + | chr3.1:37394092-37394111 | MS.gene82668:CDS | 55.0% |
| !! | GCAAGGTGCTGCTTACGTCT+CGG | + | chr3.1:37394023-37394042 | MS.gene82668:intron | 55.0% |
| ATGCGTTCTGCGGAGACCGA+AGG | + | chr3.1:37394186-37394205 | MS.gene82668:CDS | 60.0% | |
| CGTCTCTTACGACCACACCC+GGG | - | chr3.1:37393574-37393593 | None:intergenic | 60.0% | |
| GCGTCTCTTACGACCACACC+CGG | - | chr3.1:37393575-37393594 | None:intergenic | 60.0% | |
| GGGGAAGCAGATGCGTTCTG+CGG | + | chr3.1:37394176-37394195 | MS.gene82668:CDS | 60.0% | |
| GTCTCTTACGACCACACCCG+GGG | - | chr3.1:37393573-37393592 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 37393563 | 37394557 | 37393563 | ID=MS.gene82668 |
| chr3.1 | mRNA | 37393563 | 37394557 | 37393563 | ID=MS.gene82668.t1;Parent=MS.gene82668 |
| chr3.1 | exon | 37393563 | 37393853 | 37393563 | ID=MS.gene82668.t1.exon1;Parent=MS.gene82668.t1 |
| chr3.1 | CDS | 37393563 | 37393853 | 37393563 | ID=cds.MS.gene82668.t1;Parent=MS.gene82668.t1 |
| chr3.1 | exon | 37394024 | 37394207 | 37394024 | ID=MS.gene82668.t1.exon2;Parent=MS.gene82668.t1 |
| chr3.1 | CDS | 37394024 | 37394207 | 37394024 | ID=cds.MS.gene82668.t1;Parent=MS.gene82668.t1 |
| chr3.1 | exon | 37394460 | 37394557 | 37394460 | ID=MS.gene82668.t1.exon3;Parent=MS.gene82668.t1 |
| chr3.1 | CDS | 37394460 | 37394557 | 37394460 | ID=cds.MS.gene82668.t1;Parent=MS.gene82668.t1 |
| Gene Sequence |
| Protein sequence |
