Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83155.t1 | NP_001235961.1 | 100 | 51 | 0 | 0 | 1 | 51 | 47 | 97 | 3.10E-18 | 100.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83155.t1 | Q9LMN4 | 94.0 | 50 | 3 | 0 | 1 | 50 | 47 | 96 | 6.2e-20 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83155.t1 | A0A0B2SAJ4 | 100.0 | 51 | 0 | 0 | 1 | 51 | 47 | 97 | 2.2e-18 | 100.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene83155 | 0.818012 | 2.45E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene83155 | 0.815883 | 7.36E-52 | -1.69E-46 |
| MS.gene050320 | MS.gene83155 | 0.807436 | 5.06E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene83155 | 0.818067 | 2.38E-52 | -1.69E-46 |
| MS.gene051746 | MS.gene83155 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene83155 | 0.814093 | 1.84E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene83155 | 0.838733 | 2.41E-57 | -1.69E-46 |
| MS.gene052947 | MS.gene83155 | 0.818656 | 1.75E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene83155 | 0.808416 | 3.13E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene83155 | 0.823044 | 1.71E-53 | -1.69E-46 |
| MS.gene055424 | MS.gene83155 | 0.813833 | 2.09E-51 | -1.69E-46 |
| MS.gene056631 | MS.gene83155 | 0.822089 | 2.85E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene83155 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene83155 | 0.807331 | 5.33E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene83155 | 0.829864 | 4.05E-55 | -1.69E-46 |
| MS.gene057999 | MS.gene83155 | 0.828777 | 7.44E-55 | -1.69E-46 |
| MS.gene058074 | MS.gene83155 | 0.805731 | 1.16E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene83155 | 0.812864 | 3.42E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene83155 | 0.814561 | 1.45E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene83155 | 0.801293 | 9.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83155.t1 | MTR_4g083170 | 100.000 | 51 | 0 | 0 | 1 | 51 | 48 | 98 | 7.33e-30 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83155.t1 | AT1G21190 | 94.000 | 50 | 3 | 0 | 1 | 50 | 47 | 96 | 2.58e-28 | 96.7 |
| MS.gene83155.t1 | AT1G76860 | 92.157 | 51 | 4 | 0 | 1 | 51 | 47 | 97 | 1.69e-27 | 94.7 |
Find 15 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTCCGTTCCTGTTTGTT+AGG | 0.219777 | 4.2:-26357600 | MS.gene83155:CDS |
| CAACATCTTAATATGATTCT+TGG | 0.270259 | 4.2:-26358837 | None:intergenic |
| CGCCATCTCCCCTAACAAAC+AGG | 0.401332 | 4.2:+26357590 | None:intergenic |
| TTCCTGTTTGTTAGGGGAGA+TGG | 0.402900 | 4.2:-26357592 | MS.gene83155:CDS |
| TAGGGGAGATGGCGTGATAT+TGG | 0.403792 | 4.2:-26357581 | MS.gene83155:CDS |
| AGTTCCGTTCCTGTTTGTTA+GGG | 0.430031 | 4.2:-26357599 | MS.gene83155:CDS |
| AGACATACGAAGAAATTGTC+AGG | 0.452612 | 4.2:-26358773 | MS.gene83155:CDS |
| GTTCCGTTCCTGTTTGTTAG+GGG | 0.578832 | 4.2:-26357598 | MS.gene83155:CDS |
| CCATGTTATGCAGTCCTCAG+TGG | 0.619717 | 4.2:+26357550 | None:intergenic |
| TGTTATGCAGTCCTCAGTGG+TGG | 0.628383 | 4.2:+26357553 | None:intergenic |
| TGTTGAAGAAATTGTTACAA+CGG | 0.651927 | 4.2:-26358811 | MS.gene83155:CDS |
| GACATACGAAGAAATTGTCA+GGG | 0.666267 | 4.2:-26358772 | MS.gene83155:intron |
| TCTCCCCTAACAAACAGGAA+CGG | 0.688766 | 4.2:+26357595 | None:intergenic |
| TCTGATTGCAGACTACAAAG+CGG | 0.697746 | 4.2:-26357624 | MS.gene83155:intron |
| TATTGGTTTCTCCACCACTG+AGG | 0.734600 | 4.2:-26357564 | MS.gene83155:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAAACTTTTGAATAAAATT+TGG | - | chr4.2:26358377-26358396 | MS.gene83155:intron | 15.0% |
| !! | AATTTAAATTCTCCAATTTG+AGG | - | chr4.2:26358114-26358133 | MS.gene83155:intron | 20.0% |
| !! | CAAAATGGTCTATAAAAAAA+AGG | - | chr4.2:26358676-26358695 | MS.gene83155:intron | 20.0% |
| !! | GAACTAAAAAATTGAAATAG+CGG | - | chr4.2:26358136-26358155 | MS.gene83155:intron | 20.0% |
| !!! | AATTTTTTAGTTCCTCAAAT+TGG | + | chr4.2:26358129-26358148 | None:intergenic | 20.0% |
| !!! | ATCACAATTCTGAAAAAATA+AGG | + | chr4.2:26358532-26358551 | None:intergenic | 20.0% |
| !!! | TTGAGAGTATTATAAAATGA+GGG | + | chr4.2:26358211-26358230 | None:intergenic | 20.0% |
| !!! | TTTTGAATAAAATTTGGTTG+TGG | - | chr4.2:26358383-26358402 | MS.gene83155:intron | 20.0% |
| !!! | TTTTTTATAGACCATTTTGA+TGG | + | chr4.2:26358675-26358694 | None:intergenic | 20.0% |
| ! | AGAAAAACAAAAATCATCCA+AGG | + | chr4.2:26358736-26358755 | None:intergenic | 25.0% |
| ! | TGTTGAAGAAATTGTTACAA+CGG | - | chr4.2:26357569-26357588 | MS.gene83155:CDS | 25.0% |
| ! | TTATTCAAAAGTTTCTCCTT+CGG | + | chr4.2:26358373-26358392 | None:intergenic | 25.0% |
| !! | AAGGATTATTGTAGTTTGTA+AGG | - | chr4.2:26357736-26357755 | MS.gene83155:intron | 25.0% |
| !! | TAAAAAATTGAAATAGCGGA+GGG | - | chr4.2:26358140-26358159 | MS.gene83155:intron | 25.0% |
| !!! | ATTTTGAAGATACTTTTCCT+TGG | - | chr4.2:26358716-26358735 | MS.gene83155:intron | 25.0% |
| !!! | GTTGAGAGTATTATAAAATG+AGG | + | chr4.2:26358212-26358231 | None:intergenic | 25.0% |
| !!! | TATTTTTTCGATTAGATTGC+TGG | - | chr4.2:26358486-26358505 | MS.gene83155:intron | 25.0% |
| !!! | TGAGAGTATTATAAAATGAG+GGG | + | chr4.2:26358210-26358229 | None:intergenic | 25.0% |
| ACACCTTAAATCTGAATTGT+TGG | - | chr4.2:26357898-26357917 | MS.gene83155:intron | 30.0% | |
| AGGTCAATAGTTGAAAGAAA+AGG | + | chr4.2:26358512-26358531 | None:intergenic | 30.0% | |
| ATCCTTTATCAAACTGAAGA+AGG | + | chr4.2:26357722-26357741 | None:intergenic | 30.0% | |
| CTAAAAAATTGAAATAGCGG+AGG | - | chr4.2:26358139-26358158 | MS.gene83155:intron | 30.0% | |
| GATAAGAAAAGGATCATCTT+TGG | - | chr4.2:26358590-26358609 | MS.gene83155:intron | 30.0% | |
| TACCTTCTTCAGTTTGATAA+AGG | - | chr4.2:26357717-26357736 | MS.gene83155:intron | 30.0% | |
| ! | AAGATGATCCTTTTCTTATC+TGG | + | chr4.2:26358590-26358609 | None:intergenic | 30.0% |
| ! | AGATGATCCTTTTCTTATCT+GGG | + | chr4.2:26358589-26358608 | None:intergenic | 30.0% |
| ! | ATATCTACTAACTTGTGATC+TGG | - | chr4.2:26358550-26358569 | MS.gene83155:intron | 30.0% |
| ! | ATGCTTAGGAGAATTTTTCA+AGG | + | chr4.2:26358186-26358205 | None:intergenic | 30.0% |
| ! | TATCTACTAACTTGTGATCT+GGG | - | chr4.2:26358551-26358570 | MS.gene83155:intron | 30.0% |
| !! | ACAGTTGTTGATTTATTGTC+AGG | - | chr4.2:26357672-26357691 | MS.gene83155:intron | 30.0% |
| !! | CAGTTGTTGATTTATTGTCA+GGG | - | chr4.2:26357673-26357692 | MS.gene83155:intron | 30.0% |
| !! | GTAGTTTGTAAGGTGTTTTA+AGG | - | chr4.2:26357746-26357765 | MS.gene83155:intron | 30.0% |
| !! | TTATAGACCATTTTGATGGT+TGG | + | chr4.2:26358671-26358690 | None:intergenic | 30.0% |
| AGACATACGAAGAAATTGTC+AGG | - | chr4.2:26357607-26357626 | MS.gene83155:CDS | 35.0% | |
| ATAAAATGAGGGGAATGCTT+AGG | + | chr4.2:26358200-26358219 | None:intergenic | 35.0% | |
| ATAAATCAACAACTGTTCGC+AGG | + | chr4.2:26357668-26357687 | None:intergenic | 35.0% | |
| CTGCTTTCAATAATCATCAC+CGG | + | chr4.2:26357957-26357976 | None:intergenic | 35.0% | |
| GAAAAATAAACCGCAAAGCA+AGG | + | chr4.2:26358088-26358107 | None:intergenic | 35.0% | |
| GACATACGAAGAAATTGTCA+GGG | - | chr4.2:26357608-26357627 | MS.gene83155:CDS | 35.0% | |
| GAGAATCCTTCAACTTGATA+GGG | + | chr4.2:26358285-26358304 | None:intergenic | 35.0% | |
| GCACCAACAATTCAGATTTA+AGG | + | chr4.2:26357904-26357923 | None:intergenic | 35.0% | |
| TGACACTAACACTTCAATAC+CGG | - | chr4.2:26357935-26357954 | MS.gene83155:intron | 35.0% | |
| TGTCTTTCCCAGATAAGAAA+AGG | - | chr4.2:26358579-26358598 | MS.gene83155:intron | 35.0% | |
| TGTGTGAGTTAAGAACTATG+AGG | - | chr4.2:26357776-26357795 | MS.gene83155:intron | 35.0% | |
| !! | AGAACTATGAGGCATTGATA+CGG | - | chr4.2:26357787-26357806 | MS.gene83155:intron | 35.0% |
| AATCACGATGGGAAAGGATT+TGG | + | chr4.2:26358339-26358358 | None:intergenic | 40.0% | |
| ATCACGATGGGAAAGGATTT+GGG | + | chr4.2:26358338-26358357 | None:intergenic | 40.0% | |
| ATTGAAGTGGAGGGAAAGAA+AGG | + | chr4.2:26358307-26358326 | None:intergenic | 40.0% | |
| CTTTGTTCAGGAATCACGAT+GGG | + | chr4.2:26358350-26358369 | None:intergenic | 40.0% | |
| GGAGAATCCTTCAACTTGAT+AGG | + | chr4.2:26358286-26358305 | None:intergenic | 40.0% | |
| TCTGATTGCAGACTACAAAG+CGG | - | chr4.2:26358756-26358775 | MS.gene83155:intron | 40.0% | |
| TGTCTAGTAGCTGACATATG+TGG | + | chr4.2:26357980-26357999 | None:intergenic | 40.0% | |
| ! | AGTTCCGTTCCTGTTTGTTA+GGG | - | chr4.2:26358781-26358800 | MS.gene83155:CDS | 40.0% |
| ! | GCAGGTCTACAATTTAGCTT+TGG | + | chr4.2:26357650-26357669 | None:intergenic | 40.0% |
| !! | CTGAAATGTTGGTGCTACAT+AGG | - | chr4.2:26358010-26358029 | MS.gene83155:intron | 40.0% |
| !! | GAACTATGAGGCATTGATAC+GGG | - | chr4.2:26357788-26357807 | MS.gene83155:intron | 40.0% |
| AATAAACCGCAAAGCAAGGC+TGG | + | chr4.2:26358084-26358103 | None:intergenic | 45.0% | |
| ACGCCTTCAAGCTGAAATGT+TGG | - | chr4.2:26357999-26358018 | MS.gene83155:intron | 45.0% | |
| ATCTGCGCCAACCATCAAAA+TGG | - | chr4.2:26358661-26358680 | MS.gene83155:intron | 45.0% | |
| ATGGGAAAGGATTTGGGCAA+AGG | + | chr4.2:26358332-26358351 | None:intergenic | 45.0% | |
| CAGTTCCGTTCCTGTTTGTT+AGG | - | chr4.2:26358780-26358799 | MS.gene83155:CDS | 45.0% | |
| GCACCAACATTTCAGCTTGA+AGG | + | chr4.2:26358005-26358024 | None:intergenic | 45.0% | |
| TCTCCCCTAACAAACAGGAA+CGG | + | chr4.2:26358788-26358807 | None:intergenic | 45.0% | |
| TGATTCCTGAACAAAGCCGA+AGG | - | chr4.2:26358354-26358373 | MS.gene83155:intron | 45.0% | |
| TGGGAAAGGATTTGGGCAAA+GGG | + | chr4.2:26358331-26358350 | None:intergenic | 45.0% | |
| TGTCTAGCAGCTGACATATG+TGG | + | chr4.2:26357879-26357898 | None:intergenic | 45.0% | |
| TTCAGGAATCACGATGGGAA+AGG | + | chr4.2:26358345-26358364 | None:intergenic | 45.0% | |
| ! | CATCAATGAGCACTGCATCT+GGG | + | chr4.2:26358433-26358452 | None:intergenic | 45.0% |
| ! | GCTTTGTTCAGGAATCACGA+TGG | + | chr4.2:26358351-26358370 | None:intergenic | 45.0% |
| ! | GTTCCGTTCCTGTTTGTTAG+GGG | - | chr4.2:26358782-26358801 | MS.gene83155:CDS | 45.0% |
| ! | TCATCAATGAGCACTGCATC+TGG | + | chr4.2:26358434-26358453 | None:intergenic | 45.0% |
| ! | TTCCTGTTTGTTAGGGGAGA+TGG | - | chr4.2:26358788-26358807 | MS.gene83155:CDS | 45.0% |
| !! | CACTCTCCCTATCAAGTTGA+AGG | - | chr4.2:26358276-26358295 | MS.gene83155:intron | 45.0% |
| !! | TATTGGTTTCTCCACCACTG+AGG | - | chr4.2:26358816-26358835 | MS.gene83155:CDS | 45.0% |
| !! | TGGTGCTACATAGGTCAAGA+GGG | - | chr4.2:26358019-26358038 | MS.gene83155:intron | 45.0% |
| !! | TTGGTGCTACATAGGTCAAG+AGG | - | chr4.2:26358018-26358037 | MS.gene83155:intron | 45.0% |
| !! | TTTCTCCTTCGGCTTTGTTC+AGG | + | chr4.2:26358362-26358381 | None:intergenic | 45.0% |
| !! | TTTTGATGGTTGGCGCAGAT+TGG | + | chr4.2:26358661-26358680 | None:intergenic | 45.0% |
| AGGAGAGGTAAGCGAAAGAG+AGG | - | chr4.2:26358051-26358070 | MS.gene83155:intron | 50.0% | |
| ATAGGTCAAGAGGGGTAGGA+AGG | - | chr4.2:26358028-26358047 | MS.gene83155:intron | 50.0% | |
| CTACATAGGTCAAGAGGGGT+AGG | - | chr4.2:26358024-26358043 | MS.gene83155:intron | 50.0% | |
| GCAAAGGGGATTGAAGTGGA+GGG | + | chr4.2:26358316-26358335 | None:intergenic | 50.0% | |
| GGGAAAGGATTTGGGCAAAG+GGG | + | chr4.2:26358330-26358349 | None:intergenic | 50.0% | |
| TAGGGGAGATGGCGTGATAT+TGG | - | chr4.2:26358799-26358818 | MS.gene83155:CDS | 50.0% | |
| TAGTCTCCAGCCTTGCTTTG+CGG | - | chr4.2:26358075-26358094 | MS.gene83155:intron | 50.0% | |
| TTGGGCAAAGGGGATTGAAG+TGG | + | chr4.2:26358320-26358339 | None:intergenic | 50.0% | |
| ! | GGTGCTACATAGGTCAAGAG+GGG | - | chr4.2:26358020-26358039 | MS.gene83155:intron | 50.0% |
| CGCCATCTCCCCTAACAAAC+AGG | + | chr4.2:26358793-26358812 | None:intergenic | 55.0% | |
| GGCAAAGGGGATTGAAGTGG+AGG | + | chr4.2:26358317-26358336 | None:intergenic | 55.0% | |
| !! | CGGAGGGCTTTCGAGTGTTT+TGG | - | chr4.2:26358156-26358175 | MS.gene83155:intron | 55.0% |
| AGAGGGGTAGGAAGGAGGAG+AGG | - | chr4.2:26358036-26358055 | MS.gene83155:intron | 60.0% | |
| GGTCAAGAGGGGTAGGAAGG+AGG | - | chr4.2:26358031-26358050 | MS.gene83155:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26357555 | 26358847 | 26357555 | ID=MS.gene83155 |
| chr4.2 | mRNA | 26357555 | 26358847 | 26357555 | ID=MS.gene83155.t1;Parent=MS.gene83155 |
| chr4.2 | exon | 26358773 | 26358847 | 26358773 | ID=MS.gene83155.t1.exon1;Parent=MS.gene83155.t1 |
| chr4.2 | CDS | 26358773 | 26358847 | 26358773 | ID=cds.MS.gene83155.t1;Parent=MS.gene83155.t1 |
| chr4.2 | exon | 26357555 | 26357635 | 26357555 | ID=MS.gene83155.t1.exon2;Parent=MS.gene83155.t1 |
| chr4.2 | CDS | 26357555 | 26357635 | 26357555 | ID=cds.MS.gene83155.t1;Parent=MS.gene83155.t1 |
| Gene Sequence |
| Protein sequence |
