Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83172.t1 | XP_003607460.1 | 98.8 | 167 | 0 | 1 | 1 | 165 | 1 | 167 | 1.00E-78 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83172.t1 | B7FGK0 | 98.8 | 167 | 0 | 1 | 1 | 165 | 1 | 167 | 7.2e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene83172 | 0.816895 | 4.37E-52 | -1.69E-46 |
| MS.gene04919 | MS.gene83172 | 0.80616 | 9.41E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene83172 | 0.82757 | 1.45E-54 | -1.69E-46 |
| MS.gene049283 | MS.gene83172 | 0.81882 | 1.61E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene83172 | 0.820051 | 8.41E-53 | -1.69E-46 |
| MS.gene049680 | MS.gene83172 | 0.80299 | 4.32E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene83172 | 0.802383 | 5.76E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene83172 | 0.818197 | 2.22E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene83172 | 0.81942 | 1.17E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene83172 | 0.848805 | 4.85E-60 | -1.69E-46 |
| MS.gene053963 | MS.gene83172 | 0.832452 | 9.38E-56 | -1.69E-46 |
| MS.gene05469 | MS.gene83172 | 0.806966 | 6.36E-50 | -1.69E-46 |
| MS.gene054957 | MS.gene83172 | 0.801561 | 8.49E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene83172 | 0.833185 | 6.17E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene83172 | 0.845604 | 3.66E-59 | -1.69E-46 |
| MS.gene057208 | MS.gene83172 | 0.822868 | 1.88E-53 | -1.69E-46 |
| MS.gene057893 | MS.gene83172 | -0.809149 | 2.18E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene83172 | 0.816375 | 5.71E-52 | -1.69E-46 |
| MS.gene058592 | MS.gene83172 | 0.804771 | 1.84E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene83172 | 0.806116 | 9.62E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene83172 | 0.827374 | 1.62E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene83172 | -0.856714 | 2.67E-62 | -1.69E-46 |
| MS.gene059901 | MS.gene83172 | -0.846134 | 2.63E-59 | -1.69E-46 |
| MS.gene06011 | MS.gene83172 | 0.825786 | 3.88E-54 | -1.69E-46 |
| MS.gene060338 | MS.gene83172 | 0.801302 | 9.60E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene83172 | 0.814756 | 1.31E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene83172 | 0.828851 | 7.15E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene83172 | 0.84035 | 9.17E-58 | -1.69E-46 |
| MS.gene061188 | MS.gene83172 | 0.820028 | 8.52E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83172.t1 | MTR_4g078240 | 98.802 | 167 | 0 | 1 | 1 | 165 | 1 | 167 | 4.73e-116 | 325 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83172.t1 | AT4G03600 | 62.025 | 158 | 55 | 1 | 13 | 165 | 3 | 160 | 4.34e-68 | 204 |
| MS.gene83172.t1 | AT1G03730 | 64.417 | 163 | 54 | 2 | 7 | 165 | 3 | 165 | 9.95e-60 | 183 |
| MS.gene83172.t1 | AT4G28330 | 57.862 | 159 | 58 | 2 | 1 | 159 | 1 | 150 | 3.20e-44 | 143 |
| MS.gene83172.t1 | AT4G28340 | 57.823 | 147 | 61 | 1 | 13 | 159 | 5 | 150 | 1.35e-42 | 139 |
Find 28 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGCTGTTTGTGCTGTTT+TGG | 0.192732 | 4.2:+31792678 | MS.gene83172:CDS |
| GGTTGCTGCTTTGGTTATTA+TGG | 0.210965 | 4.2:+31792504 | MS.gene83172:CDS |
| TTCTCAAATCAATGCTTTCT+TGG | 0.227365 | 4.2:+31792392 | MS.gene83172:CDS |
| ATCTATGCATCTGTTCTTCT+TGG | 0.241680 | 4.2:+31792457 | MS.gene83172:CDS |
| AATTCCATGAAGAAGCTAAA+AGG | 0.267312 | 4.2:-31792369 | None:intergenic |
| GCTTTCTATGGTTGCTGCTT+TGG | 0.287079 | 4.2:+31792495 | MS.gene83172:CDS |
| AACAATAACATGTATGAATA+TGG | 0.311322 | 4.2:-31792289 | None:intergenic |
| AGAACAGATGCATAGATTGC+AGG | 0.399836 | 4.2:-31792451 | None:intergenic |
| GTTGAAGGAAGGAAGATTAC+TGG | 0.414092 | 4.2:+31792604 | MS.gene83172:CDS |
| TATTATGGTTCCTGCAGTTA+TGG | 0.418968 | 4.2:+31792519 | MS.gene83172:CDS |
| AGGAGGACTTTGGTGGTTGA+AGG | 0.432920 | 4.2:+31792589 | MS.gene83172:CDS |
| GAAAGCCAACAAAACAACTA+TGG | 0.434853 | 4.2:-31792554 | None:intergenic |
| GGACTTTGGTGGTTGAAGGA+AGG | 0.465297 | 4.2:+31792593 | MS.gene83172:CDS |
| TGGAAAACCTAGGAGGACTT+TGG | 0.466296 | 4.2:+31792579 | MS.gene83172:CDS |
| CAAGCTCAATCATCAACCTC+TGG | 0.488041 | 4.2:+31792424 | MS.gene83172:CDS |
| ATTCCATGAAGAAGCTAAAA+GGG | 0.497710 | 4.2:-31792368 | None:intergenic |
| TCAACCACCAAAGTCCTCCT+AGG | 0.505379 | 4.2:-31792586 | None:intergenic |
| CTAAAAGGGTACCGTAAACA+AGG | 0.549968 | 4.2:-31792354 | None:intergenic |
| AAAACCTAGGAGGACTTTGG+TGG | 0.554975 | 4.2:+31792582 | MS.gene83172:CDS |
| CTCAATCATCAACCTCTGGT+TGG | 0.564957 | 4.2:+31792428 | MS.gene83172:CDS |
| TGTTGTTGAATCGATGATGT+TGG | 0.569323 | 4.2:-31792259 | None:intergenic |
| ATCCACGTCACCATAACTGC+AGG | 0.577508 | 4.2:-31792529 | None:intergenic |
| TAATGAGCAAAAGAAGAACA+TGG | 0.605526 | 4.2:+31792317 | MS.gene83172:CDS |
| TGGCTTTCTTTGGAAAACCT+AGG | 0.614419 | 4.2:+31792569 | MS.gene83172:CDS |
| CTTTCTTTGGAAAACCTAGG+AGG | 0.626529 | 4.2:+31792572 | MS.gene83172:CDS |
| AATGAGCAAAAGAAGAACAT+GGG | 0.646111 | 4.2:+31792318 | MS.gene83172:CDS |
| ATAGATTGCAGGCCAACCAG+AGG | 0.699263 | 4.2:-31792440 | None:intergenic |
| TTCCTGCAGTTATGGTGACG+TGG | 0.716496 | 4.2:+31792527 | MS.gene83172:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAATAACATGTATGAATA+TGG | - | chr4.2:31792292-31792311 | None:intergenic | 20.0% |
| !! | ATTAAGATTTTGCTGAAAGA+AGG | + | chr4.2:31792646-31792665 | MS.gene83172:CDS | 25.0% |
| !! | TTAAGATTTTGCTGAAAGAA+GGG | + | chr4.2:31792647-31792666 | MS.gene83172:CDS | 25.0% |
| !!! | TGTCTATTTTTCCTTGTTTA+CGG | + | chr4.2:31792343-31792362 | MS.gene83172:CDS | 25.0% |
| AATGAGCAAAAGAAGAACAT+GGG | + | chr4.2:31792318-31792337 | MS.gene83172:CDS | 30.0% | |
| AATTCCATGAAGAAGCTAAA+AGG | - | chr4.2:31792372-31792391 | None:intergenic | 30.0% | |
| ATTCCATGAAGAAGCTAAAA+GGG | - | chr4.2:31792371-31792390 | None:intergenic | 30.0% | |
| TAATGAGCAAAAGAAGAACA+TGG | + | chr4.2:31792317-31792336 | MS.gene83172:CDS | 30.0% | |
| TTCTCAAATCAATGCTTTCT+TGG | + | chr4.2:31792392-31792411 | MS.gene83172:CDS | 30.0% | |
| !! | GATAACCATAGTTGTTTTGT+TGG | + | chr4.2:31792549-31792568 | MS.gene83172:CDS | 30.0% |
| !! | TTCTTCTTGGAACTGTTTTT+GGG | + | chr4.2:31792470-31792489 | MS.gene83172:CDS | 30.0% |
| !!! | GGTATTTTGTTTTGGTTAGA+AGG | + | chr4.2:31792701-31792720 | MS.gene83172:CDS | 30.0% |
| !!! | TGTTTTGGGGTATTTTGTTT+TGG | + | chr4.2:31792693-31792712 | MS.gene83172:CDS | 30.0% |
| ATCTATGCATCTGTTCTTCT+TGG | + | chr4.2:31792457-31792476 | MS.gene83172:CDS | 35.0% | |
| GAAAGCCAACAAAACAACTA+TGG | - | chr4.2:31792557-31792576 | None:intergenic | 35.0% | |
| TATTATGGTTCCTGCAGTTA+TGG | + | chr4.2:31792519-31792538 | MS.gene83172:CDS | 35.0% | |
| TGTTGTTGAATCGATGATGT+TGG | - | chr4.2:31792262-31792281 | None:intergenic | 35.0% | |
| ! | GTTCTTCTTGGAACTGTTTT+TGG | + | chr4.2:31792469-31792488 | MS.gene83172:CDS | 35.0% |
| !!! | GTTGTTTTGTTGGCTTTCTT+TGG | + | chr4.2:31792559-31792578 | MS.gene83172:CDS | 35.0% |
| !!! | TGTTTTTGGGTTGCTTTCTA+TGG | + | chr4.2:31792483-31792502 | MS.gene83172:CDS | 35.0% |
| AGAACAGATGCATAGATTGC+AGG | - | chr4.2:31792454-31792473 | None:intergenic | 40.0% | |
| GTTGAAGGAAGGAAGATTAC+TGG | + | chr4.2:31792604-31792623 | MS.gene83172:CDS | 40.0% | |
| ! | CTTTCTTTGGAAAACCTAGG+AGG | + | chr4.2:31792572-31792591 | MS.gene83172:CDS | 40.0% |
| ! | TGGCTTTCTTTGGAAAACCT+AGG | + | chr4.2:31792569-31792588 | MS.gene83172:CDS | 40.0% |
| !! | CTAAAAGGGTACCGTAAACA+AGG | - | chr4.2:31792357-31792376 | None:intergenic | 40.0% |
| !! | GGTTGCTGCTTTGGTTATTA+TGG | + | chr4.2:31792504-31792523 | MS.gene83172:CDS | 40.0% |
| !!! | GTACCCTTTTAGCTTCTTCA+TGG | + | chr4.2:31792365-31792384 | MS.gene83172:CDS | 40.0% |
| AAAACCTAGGAGGACTTTGG+TGG | + | chr4.2:31792582-31792601 | MS.gene83172:CDS | 45.0% | |
| CAAGCTCAATCATCAACCTC+TGG | + | chr4.2:31792424-31792443 | MS.gene83172:CDS | 45.0% | |
| CTCAATCATCAACCTCTGGT+TGG | + | chr4.2:31792428-31792447 | MS.gene83172:CDS | 45.0% | |
| TGGAAAACCTAGGAGGACTT+TGG | + | chr4.2:31792579-31792598 | MS.gene83172:CDS | 45.0% | |
| ! | GCTTTCTATGGTTGCTGCTT+TGG | + | chr4.2:31792495-31792514 | MS.gene83172:CDS | 45.0% |
| !!! | CTGCTGTTTGTGCTGTTTTG+GGG | + | chr4.2:31792680-31792699 | MS.gene83172:CDS | 45.0% |
| !!! | GCTGCTGTTTGTGCTGTTTT+GGG | + | chr4.2:31792679-31792698 | MS.gene83172:CDS | 45.0% |
| !!! | TGCTGCTGTTTGTGCTGTTT+TGG | + | chr4.2:31792678-31792697 | MS.gene83172:CDS | 45.0% |
| ATAGATTGCAGGCCAACCAG+AGG | - | chr4.2:31792443-31792462 | None:intergenic | 50.0% | |
| ATCCACGTCACCATAACTGC+AGG | - | chr4.2:31792532-31792551 | None:intergenic | 50.0% | |
| TCAACCACCAAAGTCCTCCT+AGG | - | chr4.2:31792589-31792608 | None:intergenic | 50.0% | |
| ! | AGGAGGACTTTGGTGGTTGA+AGG | + | chr4.2:31792589-31792608 | MS.gene83172:CDS | 50.0% |
| ! | GGACTTTGGTGGTTGAAGGA+AGG | + | chr4.2:31792593-31792612 | MS.gene83172:CDS | 50.0% |
| ! | TTCCTGCAGTTATGGTGACG+TGG | + | chr4.2:31792527-31792546 | MS.gene83172:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 31792238 | 31792735 | 31792238 | ID=MS.gene83172 |
| chr4.2 | mRNA | 31792238 | 31792735 | 31792238 | ID=MS.gene83172.t1;Parent=MS.gene83172 |
| chr4.2 | exon | 31792238 | 31792735 | 31792238 | ID=MS.gene83172.t1.exon1;Parent=MS.gene83172.t1 |
| chr4.2 | CDS | 31792238 | 31792735 | 31792238 | ID=cds.MS.gene83172.t1;Parent=MS.gene83172.t1 |
| Gene Sequence |
| Protein sequence |
