Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83221.t1 | XP_003605725.1 | 84.1 | 138 | 20 | 1 | 1 | 136 | 1 | 138 | 1.00E-44 | 189.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83221.t1 | G7JSI0 | 84.1 | 138 | 20 | 1 | 1 | 136 | 1 | 138 | 7.4e-45 | 189.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene83221 | 0.851822 | 6.91E-61 | -1.69E-46 |
| MS.gene050563 | MS.gene83221 | 0.836971 | 6.85E-57 | -1.69E-46 |
| MS.gene050584 | MS.gene83221 | 0.866207 | 3.37E-65 | -1.69E-46 |
| MS.gene050585 | MS.gene83221 | 0.866778 | 2.22E-65 | -1.69E-46 |
| MS.gene050586 | MS.gene83221 | 0.828128 | 1.07E-54 | -1.69E-46 |
| MS.gene050587 | MS.gene83221 | 0.849351 | 3.42E-60 | -1.69E-46 |
| MS.gene050807 | MS.gene83221 | 0.809758 | 1.62E-50 | -1.69E-46 |
| MS.gene05143 | MS.gene83221 | 0.820067 | 8.34E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene83221 | 0.854872 | 9.22E-62 | -1.69E-46 |
| MS.gene052142 | MS.gene83221 | 0.888487 | 5.65E-73 | -1.69E-46 |
| MS.gene052187 | MS.gene83221 | 0.824202 | 9.17E-54 | -1.69E-46 |
| MS.gene053074 | MS.gene83221 | 0.879025 | 1.74E-69 | -1.69E-46 |
| MS.gene053383 | MS.gene83221 | 0.871454 | 6.75E-67 | -1.69E-46 |
| MS.gene05456 | MS.gene83221 | 0.856677 | 2.74E-62 | -1.69E-46 |
| MS.gene054596 | MS.gene83221 | 0.822262 | 2.60E-53 | -1.69E-46 |
| MS.gene055359 | MS.gene83221 | 0.895624 | 8.03E-76 | -1.69E-46 |
| MS.gene058168 | MS.gene83221 | 0.81483 | 1.26E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene83221 | 0.89051 | 9.23E-74 | -1.69E-46 |
| MS.gene059124 | MS.gene83221 | 0.816684 | 4.87E-52 | -1.69E-46 |
| MS.gene059632 | MS.gene83221 | 0.809259 | 2.07E-50 | -1.69E-46 |
| MS.gene059768 | MS.gene83221 | 0.814221 | 1.72E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene83221 | 0.815139 | 1.08E-51 | -1.69E-46 |
| MS.gene060691 | MS.gene83221 | 0.806279 | 8.88E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83221.t1 | MTR_4g037560 | 83.333 | 138 | 21 | 1 | 1 | 136 | 1 | 138 | 1.11e-64 | 193 |
| MS.gene83221.t1 | MTR_4g037480 | 77.372 | 137 | 20 | 2 | 1 | 136 | 1 | 127 | 2.33e-60 | 182 |
| MS.gene83221.t1 | MTR_2g090185 | 53.968 | 126 | 49 | 4 | 1 | 126 | 1 | 117 | 5.24e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83221.t1 | AT2G20870 | 39.048 | 105 | 61 | 3 | 23 | 125 | 23 | 126 | 5.67e-12 | 59.3 |
Find 0 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTACATTATCCAGGCAT+TGG | - | 33026:2654-2673 | MS.gene83221:CDS | 35.0% | |
| ACTCCTAATACCCCATTCAT+TGG | - | 33026:2708-2727 | MS.gene83221:CDS | 40.0% | |
| CATGGGAAACTACATTATCC+AGG | - | 33026:2648-2667 | MS.gene83221:CDS | 40.0% | |
| TTAGGAGTAAGTCCAAAGTG+TGG | + | 33026:2696-2715 | MS.gene83221:intergenic | 40.0% | |
| TTCTGTTCAAGCACGAACAT+GGG | - | 33026:2631-2650 | MS.gene83221:CDS | 40.0% | |
| TTTCTGTTCAAGCACGAACA+TGG | - | 33026:2630-2649 | MS.gene83221:CDS | 40.0% | |
| !! | ATCCAGGCATTGGACATTTA+GGG | - | 33026:2664-2683 | MS.gene83221:CDS | 40.0% |
| !! | TATCCAGGCATTGGACATTT+AGG | - | 33026:2663-2682 | MS.gene83221:CDS | 40.0% |
| AACCCTAAATGTCCAATGCC+TGG | + | 33026:2669-2688 | MS.gene83221:intergenic | 45.0% | |
| ACAACACAACAAGCAGTTGC+TGG | - | 33026:2558-2577 | MS.gene83221:CDS | 45.0% | |
| AGTTCAGGATCAGGATCATC+GGG | - | 33026:2771-2790 | MS.gene83221:CDS | 45.0% | |
| CCTAATACCCCATTCATTGG+TGG | - | 33026:2711-2730 | MS.gene83221:CDS | 45.0% | |
| CTCTTTCTTGTCCTCGTTGT+CGG | + | 33026:2604-2623 | MS.gene83221:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+TGG | - | 33026:2819-2838 | MS.gene83221:CDS | 45.0% | |
| GAGTAAGTCCAAAGTGTGGT+GGG | + | 33026:2692-2711 | MS.gene83221:intergenic | 45.0% | |
| GAGTTCAGGATCAGGATCAT+CGG | - | 33026:2770-2789 | MS.gene83221:CDS | 45.0% | |
| TCGTGCTTGAACAGAAACTG+AGG | + | 33026:2627-2646 | MS.gene83221:intergenic | 45.0% | |
| ! | CCACCAATGAATGGGGTATT+AGG | + | 33026:2714-2733 | MS.gene83221:intergenic | 45.0% |
| !! | CTGCTTGTTGTGTTGTGAGA+AGG | + | 33026:2554-2573 | MS.gene83221:intergenic | 45.0% |
| ACAAATGTGTCATCGCCACC+AGG | + | 33026:2810-2829 | MS.gene83221:intergenic | 50.0% | |
| ACACCACCACTTCCAGGATT+CGG | + | 33026:2855-2874 | MS.gene83221:intergenic | 50.0% | |
| CTCCAGTGCCACCAATGAAT+GGG | + | 33026:2722-2741 | MS.gene83221:intergenic | 50.0% | |
| GATTCGGAACCTCAAAGCCA+GGG | + | 33026:2839-2858 | MS.gene83221:intergenic | 50.0% | |
| GGAGTAAGTCCAAAGTGTGG+TGG | + | 33026:2693-2712 | MS.gene83221:intergenic | 50.0% | |
| GGTCACAGTTATGTTCCTGG+TGG | - | 33026:2792-2811 | MS.gene83221:CDS | 50.0% | |
| TCCAGTGCCACCAATGAATG+GGG | + | 33026:2721-2740 | MS.gene83221:intergenic | 50.0% | |
| TCGGGTCACAGTTATGTTCC+TGG | - | 33026:2789-2808 | MS.gene83221:CDS | 50.0% | |
| TGCTAAATCCACCGACAACG+AGG | - | 33026:2590-2609 | MS.gene83221:CDS | 50.0% | |
| TTAGGGTTCCCACCACACTT+TGG | - | 33026:2681-2700 | MS.gene83221:CDS | 50.0% | |
| TTTCTTGTCCTCGTTGTCGG+TGG | + | 33026:2601-2620 | MS.gene83221:intergenic | 50.0% | |
| ! | AATCCTGGAAGTGGTGGTGT+CGG | - | 33026:2855-2874 | MS.gene83221:CDS | 50.0% |
| ! | TCAGGATCAGGTTCGAGTTC+AGG | - | 33026:2756-2775 | MS.gene83221:CDS | 50.0% |
| ! | TCAGGTTCGAGTTCAGGATC+AGG | - | 33026:2762-2781 | MS.gene83221:CDS | 50.0% |
| !! | TGTTCCAAACCCTGGCTTTG+AGG | - | 33026:2827-2846 | MS.gene83221:CDS | 50.0% |
| CCTCCAGTGCCACCAATGAA+TGG | + | 33026:2723-2742 | MS.gene83221:intergenic | 55.0% | |
| GAGGTTCCGAATCCTGGAAG+TGG | - | 33026:2846-2865 | MS.gene83221:CDS | 55.0% | |
| GGAACCTCAAAGCCAGGGTT+TGG | + | 33026:2834-2853 | MS.gene83221:intergenic | 55.0% | |
| GGATTCGGAACCTCAAAGCC+AGG | + | 33026:2840-2859 | MS.gene83221:intergenic | 55.0% | |
| GTTCCGAATCCTGGAAGTGG+TGG | - | 33026:2849-2868 | MS.gene83221:CDS | 55.0% | |
| ! | ACCCCATTCATTGGTGGCAC+TGG | - | 33026:2717-2736 | MS.gene83221:CDS | 55.0% |
| ! | ATTGGTGGCACTGGAGGATC+AGG | - | 33026:2726-2745 | MS.gene83221:CDS | 55.0% |
| ! | CCATTCATTGGTGGCACTGG+AGG | - | 33026:2720-2739 | MS.gene83221:CDS | 55.0% |
| ! | TGGCACTGGAGGATCAGGAT+CGG | - | 33026:2731-2750 | MS.gene83221:CDS | 55.0% |
| !! | GGCTTTGAGGTTCCGAATCC+TGG | - | 33026:2840-2859 | MS.gene83221:CDS | 55.0% |
| TCAGGATCGGGCTCAGGATC+AGG | - | 33026:2744-2763 | MS.gene83221:CDS | 60.0% | |
| ! | GGCACTGGAGGATCAGGATC+GGG | - | 33026:2732-2751 | MS.gene83221:CDS | 60.0% |
| CCACCGACACCACCACTTCC+AGG | + | 33026:2861-2880 | MS.gene83221:intergenic | 65.0% | |
| GGAGGATCAGGATCGGGCTC+AGG | - | 33026:2738-2757 | MS.gene83221:CDS | 65.0% | |
| ! | CCTGGAAGTGGTGGTGTCGG+TGG | - | 33026:2858-2877 | MS.gene83221:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33026 | gene | 2498 | 2908 | 2498 | ID=MS.gene83221 |
| 33026 | mRNA | 2498 | 2908 | 2498 | ID=MS.gene83221.t1;Parent=MS.gene83221 |
| 33026 | exon | 2498 | 2908 | 2498 | ID=MS.gene83221.t1.exon1;Parent=MS.gene83221.t1 |
| 33026 | CDS | 2498 | 2908 | 2498 | ID=cds.MS.gene83221.t1;Parent=MS.gene83221.t1 |
| Gene Sequence |
| Protein sequence |
