AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83461

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83461.t1 MTR_5g026320 95.852 458 19 0 1 458 1 458 0.0 910
MS.gene83461.t1 MTR_3g450710 36.449 428 245 12 53 456 44 468 3.35e-74 241
MS.gene83461.t1 MTR_5g090830 35.991 439 250 13 15 428 7 439 2.01e-73 239
MS.gene83461.t1 MTR_5g090810 35.927 437 253 14 15 428 7 439 1.05e-72 237
MS.gene83461.t1 MTR_3g450690 36.893 412 236 12 67 457 65 473 5.18e-71 233
MS.gene83461.t1 MTR_5g090880 37.688 398 221 12 56 428 46 441 6.31e-71 232
MS.gene83461.t1 MTR_7g114470 26.018 442 264 18 29 437 20 431 5.23e-27 113
MS.gene83461.t1 MTR_5g076700 24.779 452 280 16 16 437 8 429 1.05e-26 112
MS.gene83461.t1 MTR_4g007540 25.178 421 280 13 42 444 25 428 1.59e-26 111
MS.gene83461.t1 MTR_8g096530 26.185 401 258 13 41 424 28 407 5.84e-26 110
MS.gene83461.t1 MTR_1g053315 25.349 430 278 17 34 437 35 447 1.15e-25 109
MS.gene83461.t1 MTR_2g064495 23.733 434 293 15 22 437 7 420 1.69e-25 108
MS.gene83461.t1 MTR_2g105330 24.495 396 271 14 21 401 5 387 1.12e-24 106
MS.gene83461.t1 MTR_4g022290 26.170 470 275 23 17 447 3 439 8.01e-24 103
MS.gene83461.t1 MTR_5g084870 25.293 427 273 13 17 426 2 399 8.99e-24 103
MS.gene83461.t1 MTR_3g098980 24.627 402 262 14 41 424 35 413 1.90e-23 102
MS.gene83461.t1 MTR_4g127650 25.980 408 263 15 19 397 9 406 2.01e-23 102
MS.gene83461.t1 MTR_8g079970 24.324 444 262 17 17 426 2 405 3.53e-23 102
MS.gene83461.t1 MTR_4g097090 26.400 375 235 15 53 397 42 405 4.41e-23 101
MS.gene83461.t1 MTR_7g084940 27.056 377 236 13 41 399 37 392 5.23e-23 101
MS.gene83461.t1 MTR_3g049150 25.301 415 277 12 52 443 36 440 5.97e-23 101
MS.gene83461.t1 MTR_3g112180 26.000 400 245 13 33 405 25 400 6.76e-23 101
MS.gene83461.t1 MTR_7g027175 25.313 399 262 14 41 423 37 415 1.41e-22 100
MS.gene83461.t1 MTR_8g075610 24.332 374 256 13 42 401 16 376 1.94e-21 96.7
MS.gene83461.t1 MTR_7g114550 26.355 406 248 17 32 414 22 399 2.03e-21 97.1
MS.gene83461.t1 MTR_8g079980 26.077 441 257 16 17 426 2 404 4.11e-21 95.9
MS.gene83461.t1 MTR_1g040215 27.073 410 249 19 16 402 7 389 4.72e-21 95.9
MS.gene83461.t1 MTR_7g085990 27.315 432 239 22 32 417 18 420 5.74e-21 95.5
MS.gene83461.t1 MTR_7g114560 24.566 403 259 16 32 414 22 399 1.04e-20 94.7
MS.gene83461.t1 MTR_7g086000 24.096 415 284 14 55 443 41 450 3.13e-20 93.2
MS.gene83461.t1 MTR_7g114520 25.000 396 252 15 32 404 23 396 3.47e-20 93.2
MS.gene83461.t1 MTR_8g079950 23.973 438 269 17 17 424 2 405 3.61e-20 93.2
MS.gene83461.t1 MTR_3g101900 26.747 415 261 17 58 448 48 443 9.51e-20 91.7
MS.gene83461.t1 MTR_7g114490 25.187 401 259 15 32 414 22 399 1.19e-19 91.7
MS.gene83461.t1 MTR_3g086020 24.402 418 231 19 15 400 2 366 6.38e-19 89.0
MS.gene83461.t1 MTR_8g467590 26.632 383 214 22 51 400 38 386 1.96e-18 87.8
MS.gene83461.t1 MTR_1g040175 26.884 398 233 19 33 402 22 389 4.89e-18 86.7
MS.gene83461.t1 MTR_0190s0020 25.000 456 295 18 18 448 4 437 1.01e-17 85.5
MS.gene83461.t1 MTR_8g467580 26.247 381 226 15 47 400 34 386 6.08e-17 83.2
MS.gene83461.t1 MTR_3g102010 26.389 432 267 18 46 448 37 446 6.74e-17 83.2
MS.gene83461.t1 MTR_4g094842 23.656 465 296 17 18 448 4 443 6.87e-17 83.2
MS.gene83461.t1 MTR_7g056087 23.706 367 250 13 18 361 4 363 7.20e-17 82.8
MS.gene83461.t1 MTR_3g465990 22.642 318 229 8 56 358 47 362 2.70e-16 81.3
MS.gene83461.t1 MTR_5g084850 23.131 428 280 13 17 426 2 398 5.60e-16 80.1
MS.gene83461.t1 MTR_3g465980 23.140 363 259 11 13 358 3 362 6.52e-16 80.1
MS.gene83461.t1 MTR_1g081660 24.682 393 252 17 41 414 39 406 1.68e-15 79.0
MS.gene83461.t1 MTR_6g015320 25.592 422 256 17 41 424 29 430 2.52e-15 78.2
MS.gene83461.t1 MTR_1g040315 25.899 278 169 10 16 272 7 268 2.66e-15 78.2
MS.gene83461.t1 MTR_3g026950 21.226 424 297 13 35 430 17 431 3.31e-15 77.8
MS.gene83461.t1 MTR_7g084910 25.397 378 227 14 41 399 38 379 3.34e-15 77.8
MS.gene83461.t1 MTR_4g094835 23.245 413 250 17 71 448 61 441 3.64e-15 77.8
MS.gene83461.t1 MTR_3g071320 23.934 422 286 14 23 423 8 415 8.11e-15 76.6
MS.gene83461.t1 MTR_7g114960 22.118 321 222 11 56 361 45 352 8.37e-15 76.6
MS.gene83461.t1 MTR_3g465910 23.677 359 228 10 12 340 2 344 1.84e-14 75.9
MS.gene83461.t1 MTR_1g094790 25.527 427 238 21 17 400 2 391 9.65e-14 73.6
MS.gene83461.t1 MTR_6g053810 23.607 377 233 12 14 355 12 368 1.19e-13 73.2
MS.gene83461.t1 MTR_6g015315 26.265 415 255 19 45 424 30 428 6.76e-13 70.9
MS.gene83461.t1 MTR_2g036780 26.829 246 157 10 40 272 34 269 9.11e-13 70.5
MS.gene83461.t1 MTR_3g465880 22.727 374 246 11 13 355 3 364 2.98e-12 68.9
MS.gene83461.t1 MTR_4g094832 24.324 370 229 16 103 448 83 425 4.87e-12 68.2
MS.gene83461.t1 MTR_2g015430 20.354 339 246 9 74 400 58 384 2.24e-11 65.9
MS.gene83461.t1 MTR_7g014250 23.543 429 262 19 41 424 26 433 5.43e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83461.t1 AT1G31490 58.296 446 176 8 16 458 4 442 0.0 554
MS.gene83461.t1 AT1G78990 39.119 386 221 11 53 430 46 425 1.85e-76 246
MS.gene83461.t1 AT5G16410 36.406 434 257 13 8 430 26 451 2.05e-74 241
MS.gene83461.t1 AT1G32910 36.735 392 227 12 53 430 46 430 2.84e-72 236
MS.gene83461.t1 AT5G41040 28.346 381 231 13 41 401 38 396 5.86e-28 115
MS.gene83461.t1 AT5G41040 28.346 381 231 13 41 401 54 412 8.12e-28 115
MS.gene83461.t1 AT1G27620 25.907 386 247 13 32 400 21 384 6.03e-24 104
MS.gene83461.t1 AT3G48720 25.500 400 216 16 41 401 30 386 2.43e-23 102
MS.gene83461.t1 AT2G19070 26.768 396 230 13 48 397 20 401 3.04e-23 102
MS.gene83461.t1 AT3G30280 25.882 425 261 20 14 411 2 399 1.72e-22 100
MS.gene83461.t1 AT5G48930 25.189 397 261 14 24 401 9 388 2.07e-22 99.8
MS.gene83461.t1 AT1G03390 26.531 392 241 19 35 400 37 407 2.67e-22 99.8
MS.gene83461.t1 AT5G63560 25.616 406 258 14 14 401 3 382 3.93e-22 98.6
MS.gene83461.t1 AT1G24430 25.598 418 263 15 27 428 18 403 4.24e-22 98.6
MS.gene83461.t1 AT3G26040 25.336 446 259 15 17 428 2 407 5.24e-22 98.6
MS.gene83461.t1 AT5G57840 25.070 359 244 12 17 360 2 350 7.52e-22 98.2
MS.gene83461.t1 AT5G47950 24.533 428 278 18 14 428 2 397 1.79e-21 96.7
MS.gene83461.t1 AT5G42830 23.628 419 270 15 58 448 48 444 8.56e-21 95.1
MS.gene83461.t1 AT3G03480 25.814 430 266 20 33 436 32 434 4.36e-20 92.8
MS.gene83461.t1 AT3G50270 23.467 473 279 18 18 445 6 440 1.99e-19 90.9
MS.gene83461.t1 AT5G67150 25.117 426 244 21 17 400 4 396 1.06e-18 88.6
MS.gene83461.t1 AT1G24420 29.394 330 191 15 18 327 5 312 1.41e-18 88.2
MS.gene83461.t1 AT1G24420 29.394 330 191 15 18 327 5 312 1.41e-18 88.2
MS.gene83461.t1 AT4G15390 24.634 410 266 16 12 400 2 389 1.56e-18 88.2
MS.gene83461.t1 AT4G15390 24.634 410 266 16 12 400 2 389 1.56e-18 88.2
MS.gene83461.t1 AT2G40230 24.619 394 252 15 35 404 20 392 2.92e-18 87.0
MS.gene83461.t1 AT1G65450 23.280 378 254 15 41 399 40 400 1.62e-16 82.0
MS.gene83461.t1 AT3G50280 25.120 418 252 21 17 400 3 393 2.17e-16 81.6
MS.gene83461.t1 AT5G67160 24.783 460 284 18 17 449 4 428 2.63e-15 78.2
MS.gene83461.t1 AT4G31910 26.510 298 185 12 30 297 23 316 3.73e-15 77.8
MS.gene83461.t1 AT5G07860 20.896 402 274 11 68 448 66 444 8.93e-15 76.6
MS.gene83461.t1 AT1G28680 23.429 350 221 13 74 400 62 387 1.18e-13 73.2
MS.gene83461.t1 AT5G07850 23.270 477 298 18 17 454 7 454 1.24e-13 73.2
MS.gene83461.t1 AT1G28680 23.301 309 199 11 74 360 62 354 3.81e-12 68.2

Find 79 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAAATACCAACCTTATT+TGG 0.132764 5.4:+17298153 MS.gene83461:CDS
CGGAGACTTGCTTCTGTTAA+TGG 0.217032 5.4:+17298286 MS.gene83461:CDS
GAGAGATTAATCACTGAAAA+TGG 0.231244 5.4:-17297641 None:intergenic
TCAAATCTTGATCTACTTTC+AGG 0.243930 5.4:+17297662 MS.gene83461:CDS
TCCTGTTAGTGAAGTTGATT+TGG 0.253495 5.4:+17298699 MS.gene83461:CDS
CATTGGTAATGTATTGTCTT+TGG 0.262038 5.4:+17298462 MS.gene83461:CDS
GGATTTGGAAGTCCTTTGTT+AGG 0.289603 5.4:+17298721 MS.gene83461:CDS
AGCTTCAATCTTTGTTCTCT+TGG 0.297597 5.4:-17298311 None:intergenic
TTCAAAGCATCTACTAATTC+AGG 0.309519 5.4:-17298844 None:intergenic
CCTGTCTTAGTGGTTTCTTC+AGG 0.314491 5.4:+17298667 MS.gene83461:CDS
AGATTTCCCTTTACAAATTC+AGG 0.320821 5.4:+17297964 MS.gene83461:CDS
TAGAAATACAAGTATGTAAC+TGG 0.322652 5.4:-17297692 None:intergenic
TTAGGTGATGCTACTTCATT+TGG 0.333118 5.4:+17298040 MS.gene83461:CDS
TGAATTTGTAAAGGGAAATC+TGG 0.334806 5.4:-17297962 None:intergenic
TTGATTGGATTGAGTTTCAT+AGG 0.350449 5.4:+17298599 MS.gene83461:CDS
GATTTGAAGTTACCTAGTTT+TGG 0.354372 5.4:-17297722 None:intergenic
ATGCTTGCAAAGGCGGTGTT+AGG 0.354998 5.4:+17298631 MS.gene83461:CDS
CCTGTTAGTGAAGTTGATTT+GGG 0.355976 5.4:+17298700 MS.gene83461:CDS
ATTACTTGGATTTGATTGAT+TGG 0.362571 5.4:+17298584 MS.gene83461:CDS
CTGTTACCTGAATTTGTAAA+GGG 0.364202 5.4:-17297970 None:intergenic
AGTGAAGTTGATTTGGGATT+TGG 0.366196 5.4:+17298706 MS.gene83461:CDS
AATCATTACTATCCCTTTGC+TGG 0.371053 5.4:+17297785 MS.gene83461:CDS
CCCAAATCAACTTCACTAAC+AGG 0.388576 5.4:-17298700 None:intergenic
TTAATCACTGAAAATGGCTT+TGG 0.389224 5.4:-17297635 None:intergenic
AAGCTTTCTCTGCATATGTA+TGG 0.403699 5.4:+17298329 MS.gene83461:CDS
GTGGTAAGGGTGATGGTTCA+TGG 0.408074 5.4:+17298800 MS.gene83461:CDS
TTGTTGTCACAAACGATTTG+AGG 0.427819 5.4:-17297839 None:intergenic
TTGGTAAATTCATTTCATCT+TGG 0.434237 5.4:+17298059 MS.gene83461:CDS
TGAATTAGTAGATGCTTTGA+AGG 0.434332 5.4:+17298846 MS.gene83461:CDS
AATGTATTGTCTTTGGCTTT+CGG 0.441300 5.4:+17298469 MS.gene83461:CDS
TCTGTTACCTGAATTTGTAA+AGG 0.444738 5.4:-17297971 None:intergenic
TAGTAATGAGGAACATTACT+TGG 0.453243 5.4:+17298570 MS.gene83461:CDS
AAGATGGGTTGGTTAGTAGA+TGG 0.454588 5.4:+17298397 MS.gene83461:CDS
GAATCAAAGGCAAAGTGGTA+AGG 0.464027 5.4:+17298786 MS.gene83461:CDS
TGGTTCGGTATGAATGATAA+CGG 0.471212 5.4:-17298103 None:intergenic
AACACCAACCCTTTCAATTG+AGG 0.478455 5.4:-17298755 None:intergenic
GATTTGGAAGTCCTTTGTTA+GGG 0.486362 5.4:+17298722 MS.gene83461:CDS
CTTTGGCTTTCGGTGAGGCA+AGG 0.495189 5.4:+17298479 MS.gene83461:CDS
TGGATTGAGTTTCATAGGCC+AGG 0.499721 5.4:+17298604 MS.gene83461:CDS
AGATGATGGACCTGTCTTAG+TGG 0.507067 5.4:+17298657 MS.gene83461:CDS
CTGAAGATGAGAAGCAGTCA+TGG 0.511063 5.4:-17298887 None:intergenic
ACAAGTATGTAACTGGAAAA+CGG 0.512372 5.4:-17297685 None:intergenic
AATCAAAGGCAAAGTGGTAA+GGG 0.520980 5.4:+17298787 MS.gene83461:CDS
TGAACAATATGGCCAGCAAA+GGG 0.525017 5.4:-17297797 None:intergenic
AGGTTTGATGCTTGCAAAGG+CGG 0.529986 5.4:+17298624 MS.gene83461:CDS
AGTTCATGATGCAATTTCAA+AGG 0.532962 5.4:+17298546 MS.gene83461:CDS
AATTTCAAAGGTTAGTAATG+AGG 0.533878 5.4:+17298558 MS.gene83461:CDS
TTGAACAATATGGCCAGCAA+AGG 0.536124 5.4:-17297798 None:intergenic
AATTCAATGTCGAATTACAT+TGG 0.542017 5.4:+17298445 MS.gene83461:CDS
CAGTGTATACCTCAATTGAA+AGG 0.543563 5.4:+17298746 MS.gene83461:CDS
GATGGAAGAGAAAGAATGAG+TGG 0.544115 5.4:+17298415 MS.gene83461:CDS
GTAACAGAATTTACTTGTGG+TGG 0.546469 5.4:+17297986 MS.gene83461:CDS
AAATTTGATCCAAATAAGGT+TGG 0.546882 5.4:-17298162 None:intergenic
GGCAAGGATTCAAGAATTGA+AGG 0.552829 5.4:+17298495 MS.gene83461:CDS
GCCTTTGCAAGCATCAAACC+TGG 0.556923 5.4:-17298622 None:intergenic
GTGTTGGTTATATGAATCAA+AGG 0.560599 5.4:+17298773 MS.gene83461:CDS
AGTGTATACCTCAATTGAAA+GGG 0.563977 5.4:+17298747 MS.gene83461:CDS
TCAATTGAAAGGGTTGGTGT+TGG 0.564088 5.4:+17298757 MS.gene83461:CDS
CCTGAAGAAACCACTAAGAC+AGG 0.567163 5.4:-17298667 None:intergenic
GGACTGTTTCTGCTATCTTG+TGG 0.568637 5.4:+17298821 MS.gene83461:CDS
TAGTGGTTTCTTCAGGACAA+AGG 0.574621 5.4:+17298674 MS.gene83461:CDS
TGAGAGGCGAACGTGCGCAA+AGG 0.578264 5.4:-17298135 None:intergenic
AGGATTCAAGAATTGAAGGA+AGG 0.590702 5.4:+17298499 MS.gene83461:CDS
GCCAGGTTTGATGCTTGCAA+AGG 0.594613 5.4:+17298621 MS.gene83461:CDS
ACAAACGATTTGAGGTTCGT+CGG 0.596959 5.4:-17297831 None:intergenic
TATACCTCAATTGAAAGGGT+TGG 0.598377 5.4:+17298751 MS.gene83461:CDS
TGAAGATGAGAAGCAGTCAT+GGG 0.598746 5.4:-17298886 None:intergenic
AGGCAAAGTGGTAAGGGTGA+TGG 0.604002 5.4:+17298793 MS.gene83461:CDS
AAATAAGGTTGGTATTTGAG+AGG 0.609809 5.4:-17298151 None:intergenic
GAAGGTTCATTATCAGACAT+AGG 0.615379 5.4:+17298517 MS.gene83461:CDS
GCGGTGTTAGGACAAGATGA+TGG 0.622280 5.4:+17298643 MS.gene83461:CDS
ATTGTCTTTGGCTTTCGGTG+AGG 0.622701 5.4:+17298474 MS.gene83461:CDS
CAGGTAACAGAATTTACTTG+TGG 0.623975 5.4:+17297983 MS.gene83461:CDS
CCGCAGCATGTTAATACTCG+AGG 0.624790 5.4:-17298266 None:intergenic
TATATGAATCAAAGGCAAAG+TGG 0.632585 5.4:+17298781 MS.gene83461:CDS
CAAATCGTTTGTGACAACAA+TGG 0.655714 5.4:+17297842 MS.gene83461:CDS
GTATACACTGTCCCTAACAA+AGG 0.661728 5.4:-17298733 None:intergenic
CCTCGAGTATTAACATGCTG+CGG 0.665073 5.4:+17298266 MS.gene83461:CDS
ATGGAAGAAAATGATAAACA+CGG 0.726903 5.4:+17298348 MS.gene83461:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATTTGATCCAAATA+AGG - chr5.4:17298169-17298188 None:intergenic 15.0%
!!! TTCAAATTTTTCATATTCTT+GGG - chr5.4:17297570-17297589 None:intergenic 15.0%
!! TAAAAATCTAAATTGCTTAG+TGG - chr5.4:17297899-17297918 None:intergenic 20.0%
!!! CTTCAAATTTTTCATATTCT+TGG - chr5.4:17297571-17297590 None:intergenic 20.0%
!!! TTTTACGATTTTGAACAATA+TGG - chr5.4:17297811-17297830 None:intergenic 20.0%
! AAATTTGATCCAAATAAGGT+TGG - chr5.4:17298165-17298184 None:intergenic 25.0%
! AATTCAATGTCGAATTACAT+TGG + chr5.4:17298445-17298464 MS.gene83461:CDS 25.0%
! AATTTCAAAGGTTAGTAATG+AGG + chr5.4:17298558-17298577 MS.gene83461:CDS 25.0%
! ACTTCATAAAAAATGCAAGA+TGG + chr5.4:17298381-17298400 MS.gene83461:CDS 25.0%
! ATGGAAGAAAATGATAAACA+CGG + chr5.4:17298348-17298367 MS.gene83461:CDS 25.0%
! ATTACTTGGATTTGATTGAT+TGG + chr5.4:17298584-17298603 MS.gene83461:CDS 25.0%
! CTTCATAAAAAATGCAAGAT+GGG + chr5.4:17298382-17298401 MS.gene83461:CDS 25.0%
! TAGAAATACAAGTATGTAAC+TGG - chr5.4:17297695-17297714 None:intergenic 25.0%
! TTCTACAAAAAACCAAAACT+AGG + chr5.4:17297710-17297729 MS.gene83461:CDS 25.0%
! TTGGTAAATTCATTTCATCT+TGG + chr5.4:17298059-17298078 MS.gene83461:CDS 25.0%
!! AATTTTTATCAAATGCACCA+TGG + chr5.4:17298180-17298199 MS.gene83461:CDS 25.0%
!!! TTTACTTTTGATCATGCATT+AGG + chr5.4:17298022-17298041 MS.gene83461:CDS 25.0%
ACAAGTATGTAACTGGAAAA+CGG - chr5.4:17297688-17297707 None:intergenic 30.0%
AGATTTCCCTTTACAAATTC+AGG + chr5.4:17297964-17297983 MS.gene83461:CDS 30.0%
AGTGTATACCTCAATTGAAA+GGG + chr5.4:17298747-17298766 MS.gene83461:CDS 30.0%
AGTTCATGATGCAATTTCAA+AGG + chr5.4:17298546-17298565 MS.gene83461:CDS 30.0%
ATAAAAAATGCAAGATGGGT+TGG + chr5.4:17298386-17298405 MS.gene83461:CDS 30.0%
CTGTTACCTGAATTTGTAAA+GGG - chr5.4:17297973-17297992 None:intergenic 30.0%
GAGAGATTAATCACTGAAAA+TGG - chr5.4:17297644-17297663 None:intergenic 30.0%
TAGTAATGAGGAACATTACT+TGG + chr5.4:17298570-17298589 MS.gene83461:CDS 30.0%
TATATGAATCAAAGGCAAAG+TGG + chr5.4:17298781-17298800 MS.gene83461:CDS 30.0%
TCAAATCTTGATCTACTTTC+AGG + chr5.4:17297662-17297681 MS.gene83461:CDS 30.0%
TCTCAAATACCAACCTTATT+TGG + chr5.4:17298153-17298172 MS.gene83461:CDS 30.0%
TCTGTTACCTGAATTTGTAA+AGG - chr5.4:17297974-17297993 None:intergenic 30.0%
TGAATTAGTAGATGCTTTGA+AGG + chr5.4:17298846-17298865 MS.gene83461:CDS 30.0%
TGAATTTGTAAAGGGAAATC+TGG - chr5.4:17297965-17297984 None:intergenic 30.0%
TTAATCACTGAAAATGGCTT+TGG - chr5.4:17297638-17297657 None:intergenic 30.0%
TTGATTGGATTGAGTTTCAT+AGG + chr5.4:17298599-17298618 MS.gene83461:CDS 30.0%
! CAAAGGTTTCTTTTATGGTT+CGG - chr5.4:17298121-17298140 None:intergenic 30.0%
! CATATTTTGTATCTCTTCCA+TGG - chr5.4:17298200-17298219 None:intergenic 30.0%
! TTCAAAGCATCTACTAATTC+AGG - chr5.4:17298847-17298866 None:intergenic 30.0%
!! AAATAAGGTTGGTATTTGAG+AGG - chr5.4:17298154-17298173 None:intergenic 30.0%
!! AATGTATTGTCTTTGGCTTT+CGG + chr5.4:17298469-17298488 MS.gene83461:CDS 30.0%
!! CATTGGTAATGTATTGTCTT+TGG + chr5.4:17298462-17298481 MS.gene83461:CDS 30.0%
!! GATTTGAAGTTACCTAGTTT+TGG - chr5.4:17297725-17297744 None:intergenic 30.0%
!! GTGTTGGTTATATGAATCAA+AGG + chr5.4:17298773-17298792 MS.gene83461:CDS 30.0%
AAGCTTTCTCTGCATATGTA+TGG + chr5.4:17298329-17298348 MS.gene83461:CDS 35.0%
AATCAAAGGCAAAGTGGTAA+GGG + chr5.4:17298787-17298806 MS.gene83461:CDS 35.0%
AATCATTACTATCCCTTTGC+TGG + chr5.4:17297785-17297804 MS.gene83461:CDS 35.0%
AGCTTCAATCTTTGTTCTCT+TGG - chr5.4:17298314-17298333 None:intergenic 35.0%
AGGATTCAAGAATTGAAGGA+AGG + chr5.4:17298499-17298518 MS.gene83461:CDS 35.0%
CAAATCGTTTGTGACAACAA+TGG + chr5.4:17297842-17297861 MS.gene83461:CDS 35.0%
CAGGTAACAGAATTTACTTG+TGG + chr5.4:17297983-17298002 MS.gene83461:CDS 35.0%
CAGTGTATACCTCAATTGAA+AGG + chr5.4:17298746-17298765 MS.gene83461:CDS 35.0%
GAAGGTTCATTATCAGACAT+AGG + chr5.4:17298517-17298536 MS.gene83461:CDS 35.0%
GATTTGGAAGTCCTTTGTTA+GGG + chr5.4:17298722-17298741 MS.gene83461:CDS 35.0%
GTAACAGAATTTACTTGTGG+TGG + chr5.4:17297986-17298005 MS.gene83461:CDS 35.0%
TATACCTCAATTGAAAGGGT+TGG + chr5.4:17298751-17298770 MS.gene83461:CDS 35.0%
TTGTTGTCACAAACGATTTG+AGG - chr5.4:17297842-17297861 None:intergenic 35.0%
! CCTGTTAGTGAAGTTGATTT+GGG + chr5.4:17298700-17298719 MS.gene83461:CDS 35.0%
! TCCTGTTAGTGAAGTTGATT+TGG + chr5.4:17298699-17298718 MS.gene83461:CDS 35.0%
! TTAGGTGATGCTACTTCATT+TGG + chr5.4:17298040-17298059 MS.gene83461:CDS 35.0%
!! AGTGAAGTTGATTTGGGATT+TGG + chr5.4:17298706-17298725 MS.gene83461:CDS 35.0%
!! TGGTTCGGTATGAATGATAA+CGG - chr5.4:17298106-17298125 None:intergenic 35.0%
AACACCAACCCTTTCAATTG+AGG - chr5.4:17298758-17298777 None:intergenic 40.0%
CCCAAATCAACTTCACTAAC+AGG - chr5.4:17298703-17298722 None:intergenic 40.0%
GAATCAAAGGCAAAGTGGTA+AGG + chr5.4:17298786-17298805 MS.gene83461:CDS 40.0%
GATGGAAGAGAAAGAATGAG+TGG + chr5.4:17298415-17298434 MS.gene83461:CDS 40.0%
GCAGTCATGGGATGAAAAAT+TGG - chr5.4:17298877-17298896 None:intergenic 40.0%
GGATTTGGAAGTCCTTTGTT+AGG + chr5.4:17298721-17298740 MS.gene83461:CDS 40.0%
GGCAAGGATTCAAGAATTGA+AGG + chr5.4:17298495-17298514 MS.gene83461:CDS 40.0%
GTATACACTGTCCCTAACAA+AGG - chr5.4:17298736-17298755 None:intergenic 40.0%
TAGTGGTTTCTTCAGGACAA+AGG + chr5.4:17298674-17298693 MS.gene83461:CDS 40.0%
TGAACAATATGGCCAGCAAA+GGG - chr5.4:17297800-17297819 None:intergenic 40.0%
TGAAGATGAGAAGCAGTCAT+GGG - chr5.4:17298889-17298908 None:intergenic 40.0%
TTGAACAATATGGCCAGCAA+AGG - chr5.4:17297801-17297820 None:intergenic 40.0%
! ACAAACGATTTGAGGTTCGT+CGG - chr5.4:17297834-17297853 None:intergenic 40.0%
! GTGCGCAAAGGTTTCTTTTA+TGG - chr5.4:17298126-17298145 None:intergenic 40.0%
!! AAGATGGGTTGGTTAGTAGA+TGG + chr5.4:17298397-17298416 MS.gene83461:CDS 40.0%
!! TCAATTGAAAGGGTTGGTGT+TGG + chr5.4:17298757-17298776 MS.gene83461:CDS 40.0%
AGGTTTGATGCTTGCAAAGG+CGG + chr5.4:17298624-17298643 MS.gene83461:CDS 45.0%
CCTCGAGTATTAACATGCTG+CGG + chr5.4:17298266-17298285 MS.gene83461:CDS 45.0%
CCTGAAGAAACCACTAAGAC+AGG - chr5.4:17298670-17298689 None:intergenic 45.0%
CCTGTCTTAGTGGTTTCTTC+AGG + chr5.4:17298667-17298686 MS.gene83461:CDS 45.0%
CTGAAGATGAGAAGCAGTCA+TGG - chr5.4:17298890-17298909 None:intergenic 45.0%
TGGATTGAGTTTCATAGGCC+AGG + chr5.4:17298604-17298623 MS.gene83461:CDS 45.0%
! CGGAGACTTGCTTCTGTTAA+TGG + chr5.4:17298286-17298305 MS.gene83461:CDS 45.0%
!! AGATGATGGACCTGTCTTAG+TGG + chr5.4:17298657-17298676 MS.gene83461:CDS 45.0%
!! ATTGTCTTTGGCTTTCGGTG+AGG + chr5.4:17298474-17298493 MS.gene83461:CDS 45.0%
!! GGACTGTTTCTGCTATCTTG+TGG + chr5.4:17298821-17298840 MS.gene83461:CDS 45.0%
AGGCAAAGTGGTAAGGGTGA+TGG + chr5.4:17298793-17298812 MS.gene83461:CDS 50.0%
ATGCTTGCAAAGGCGGTGTT+AGG + chr5.4:17298631-17298650 MS.gene83461:CDS 50.0%
CCGCAGCATGTTAATACTCG+AGG - chr5.4:17298269-17298288 None:intergenic 50.0%
GCCAGGTTTGATGCTTGCAA+AGG + chr5.4:17298621-17298640 MS.gene83461:CDS 50.0%
GCCTTTGCAAGCATCAAACC+TGG - chr5.4:17298625-17298644 None:intergenic 50.0%
! GCGGTGTTAGGACAAGATGA+TGG + chr5.4:17298643-17298662 MS.gene83461:CDS 50.0%
! GTGGTAAGGGTGATGGTTCA+TGG + chr5.4:17298800-17298819 MS.gene83461:CDS 50.0%
!! CTTTGGCTTTCGGTGAGGCA+AGG + chr5.4:17298479-17298498 MS.gene83461:CDS 55.0%
TGAGAGGCGAACGTGCGCAA+AGG - chr5.4:17298138-17298157 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 17297539 17298915 17297539 ID=MS.gene83461
chr5.4 mRNA 17297539 17298915 17297539 ID=MS.gene83461.t1;Parent=MS.gene83461
chr5.4 exon 17297539 17298915 17297539 ID=MS.gene83461.t1.exon1;Parent=MS.gene83461.t1
chr5.4 CDS 17297539 17298915 17297539 ID=cds.MS.gene83461.t1;Parent=MS.gene83461.t1
Gene Sequence

>MS.gene83461

ATGTCACATATTCTCAATGATATTGCAGCCCAAGAATATGAAAAATTTGAAGTAAAGTTCATAAAGAAAAGTGTTGTAAAAGCTCTCAATCATTCACCAAAGCCATTTTCAGTGATTAATCTCTCAAATCTTGATCTACTTTCAGGCCGTTTTCCAGTTACATACTTGTATTTCTACAAAAAACCAAAACTAGGTAACTTCAAATCTTTTGTTAACAATCTTAAAACTTCTCTAGCACAAATACTCAATCATTACTATCCCTTTGCTGGCCATATTGTTCAAAATCGTAAAACCGACGAACCTCAAATCGTTTGTGACAACAATGGTTCATTAGTCATTGAAGCTCAAGTAAATATTCCACTAAGCAATTTAGATTTTTATAATCTCAATGAAACTCTTGAAGAAAAGATAGTCTTAGTTGAACCAGATTTCCCTTTACAAATTCAGGTAACAGAATTTACTTGTGGTGGAATTTCAATAGCATTTACTTTTGATCATGCATTAGGTGATGCTACTTCATTTGGTAAATTCATTTCATCTTGGTGTGAAATAGCACAAAATAAACCGTTATCATTCATACCGAACCATAAAAGAAACCTTTGCGCACGTTCGCCTCTCAAATACCAACCTTATTTGGATCAAATTTTTATCAAATGCACCATGGAAGAGATACAAAATATGAAAATAAATAAAAATATTTCGCTTAAACGTCTTTATCATATCGATGCCTCGAGTATTAACATGCTGCGGAGACTTGCTTCTGTTAATGGTACCAAGAGAACAAAGATTGAAGCTTTCTCTGCATATGTATGGAAGAAAATGATAAACACGGTTGATCAAAAACTTCATAAAAAATGCAAGATGGGTTGGTTAGTAGATGGAAGAGAAAGAATGAGTGGAAGCAAAAATTCAATGTCGAATTACATTGGTAATGTATTGTCTTTGGCTTTCGGTGAGGCAAGGATTCAAGAATTGAAGGAAGGTTCATTATCAGACATAGGTGAAATAGTTCATGATGCAATTTCAAAGGTTAGTAATGAGGAACATTACTTGGATTTGATTGATTGGATTGAGTTTCATAGGCCAGGTTTGATGCTTGCAAAGGCGGTGTTAGGACAAGATGATGGACCTGTCTTAGTGGTTTCTTCAGGACAAAGGTTTCCTGTTAGTGAAGTTGATTTGGGATTTGGAAGTCCTTTGTTAGGGACAGTGTATACCTCAATTGAAAGGGTTGGTGTTGGTTATATGAATCAAAGGCAAAGTGGTAAGGGTGATGGTTCATGGACTGTTTCTGCTATCTTGTGGCCTGAATTAGTAGATGCTTTGAAGGATGATCCAATTTTTCATCCCATGACTGCTTCTCATCTTCAGCTTTAG
Protein sequence

>MS.gene83461.t1

MSHILNDIAAQEYEKFEVKFIKKSVVKALNHSPKPFSVINLSNLDLLSGRFPVTYLYFYKKPKLGNFKSFVNNLKTSLAQILNHYYPFAGHIVQNRKTDEPQIVCDNNGSLVIEAQVNIPLSNLDFYNLNETLEEKIVLVEPDFPLQIQVTEFTCGGISIAFTFDHALGDATSFGKFISSWCEIAQNKPLSFIPNHKRNLCARSPLKYQPYLDQIFIKCTMEEIQNMKINKNISLKRLYHIDASSINMLRRLASVNGTKRTKIEAFSAYVWKKMINTVDQKLHKKCKMGWLVDGRERMSGSKNSMSNYIGNVLSLAFGEARIQELKEGSLSDIGEIVHDAISKVSNEEHYLDLIDWIEFHRPGLMLAKAVLGQDDGPVLVVSSGQRFPVSEVDLGFGSPLLGTVYTSIERVGVGYMNQRQSGKGDGSWTVSAILWPELVDALKDDPIFHPMTASHLQL