Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83849.t1 | XP_003624630.2 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 6.00E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83849.t1 | Q9LVC0 | 54.1 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 7.0e-07 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83849.t1 | G7KZH1 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 4.4e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene83849 | 0.807275 | 5.47E-50 | -1.69E-46 |
| MS.gene04915 | MS.gene83849 | 0.810882 | 9.24E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene83849 | 0.813573 | 2.39E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene83849 | 0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene83849 | 0.824651 | 7.19E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene83849 | 0.827046 | 1.94E-54 | -1.69E-46 |
| MS.gene050759 | MS.gene83849 | 0.807941 | 3.95E-50 | -1.69E-46 |
| MS.gene050941 | MS.gene83849 | 0.839046 | 2.00E-57 | -1.69E-46 |
| MS.gene051003 | MS.gene83849 | 0.805149 | 1.53E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene83849 | 0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene83849 | 0.818807 | 1.62E-52 | -1.69E-46 |
| MS.gene052342 | MS.gene83849 | 0.820371 | 7.11E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene83849 | 0.814535 | 1.47E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene83849 | 0.819935 | 8.94E-53 | -1.69E-46 |
| MS.gene053456 | MS.gene83849 | 0.818331 | 2.07E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene83849 | 0.811739 | 6.02E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene83849 | 0.837663 | 4.55E-57 | -1.69E-46 |
| MS.gene059283 | MS.gene83849 | 0.808669 | 2.76E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene83849 | 0.809768 | 1.61E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene83849 | 0.809594 | 1.75E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene83849 | 0.865203 | 7.00E-65 | -1.69E-46 |
| MS.gene060932 | MS.gene83849 | 0.862556 | 4.66E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83849.t1 | MTR_7g085780 | 98.305 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 2.48e-33 | 108 |
| MS.gene83849.t1 | MTR_6g029260 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 8.81e-15 | 61.6 |
| MS.gene83849.t1 | MTR_6g029330 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 1.94e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83849.t1 | AT5G56540 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 2.43e-14 | 60.5 |
| MS.gene83849.t1 | AT3G13520 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 4.02e-11 | 52.4 |
Find 7 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCATAGCACTTGCATT+TGG | 0.360344 | 7.4:-21725913 | MS.gene83849:CDS |
| GGCAGTTATGGTTGCTATGT+TGG | 0.451707 | 7.4:-21726028 | MS.gene83849:CDS |
| GTAGCATCAGAAGCTGGACT+TGG | 0.472931 | 7.4:+21725964 | None:intergenic |
| TCAGAAGCTGGACTTGGAGC+TGG | 0.503706 | 7.4:+21725970 | None:intergenic |
| AGTGTTGTAGCATCAGAAGC+TGG | 0.542379 | 7.4:+21725958 | None:intergenic |
| ATGAGAGAAGCAAAAGCAGT+TGG | 0.550611 | 7.4:+21725928 | None:intergenic |
| ATGTTGGTGATAGCAACAAG+TGG | 0.753532 | 7.4:-21726012 | MS.gene83849:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAGATGAACATTTTTG+TGG | - | chr7.4:21725908-21725927 | MS.gene83849:CDS | 25.0% |
| ! | GAACATTTTTGTGGCAGTTA+TGG | - | chr7.4:21725917-21725936 | MS.gene83849:CDS | 35.0% |
| ATGAGAGAAGCAAAAGCAGT+TGG | + | chr7.4:21726032-21726051 | None:intergenic | 40.0% | |
| TCTCTCATAGCACTTGCATT+TGG | - | chr7.4:21726044-21726063 | MS.gene83849:CDS | 40.0% | |
| !! | ATGTTGGTGATAGCAACAAG+TGG | - | chr7.4:21725945-21725964 | MS.gene83849:CDS | 40.0% |
| AGTGTTGTAGCATCAGAAGC+TGG | + | chr7.4:21726002-21726021 | None:intergenic | 45.0% | |
| GGCAGTTATGGTTGCTATGT+TGG | - | chr7.4:21725929-21725948 | MS.gene83849:CDS | 45.0% | |
| GTAGCATCAGAAGCTGGACT+TGG | + | chr7.4:21725996-21726015 | None:intergenic | 50.0% | |
| TCAGAAGCTGGACTTGGAGC+TGG | + | chr7.4:21725990-21726009 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 21725900 | 21726079 | 21725900 | ID=MS.gene83849 |
| chr7.4 | mRNA | 21725900 | 21726079 | 21725900 | ID=MS.gene83849.t1;Parent=MS.gene83849 |
| chr7.4 | exon | 21725900 | 21726079 | 21725900 | ID=MS.gene83849.t1.exon1;Parent=MS.gene83849.t1 |
| chr7.4 | CDS | 21725900 | 21726079 | 21725900 | ID=cds.MS.gene83849.t1;Parent=MS.gene83849.t1 |
| Gene Sequence |
| Protein sequence |
