AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84006

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84006.t1 MTR_1g050448 97.268 366 10 0 1 366 1 366 0.0 750
MS.gene84006.t1 MTR_1g112210 74.716 352 84 3 19 366 23 373 0.0 564
MS.gene84006.t1 MTR_1g112210 72.443 352 79 4 19 366 23 360 0.0 536
MS.gene84006.t1 MTR_5g071350 39.589 341 183 5 45 365 52 389 1.48e-93 285
MS.gene84006.t1 MTR_2g015720 41.144 367 182 9 9 365 6 348 7.76e-92 280
MS.gene84006.t1 MTR_2g091175 42.331 326 168 7 47 365 44 356 1.94e-89 273
MS.gene84006.t1 MTR_7g093450 40.597 335 182 6 48 365 243 577 2.72e-88 278
MS.gene84006.t1 MTR_3g049330 42.679 321 165 7 53 365 31 340 2.44e-87 268
MS.gene84006.t1 MTR_2g073510 41.846 325 171 5 49 365 29 343 4.92e-87 267
MS.gene84006.t1 MTR_0136s0010 40.000 335 184 6 48 365 165 499 3.05e-86 270
MS.gene84006.t1 MTR_3g464570 39.264 326 183 5 53 365 43 366 1.47e-85 264
MS.gene84006.t1 MTR_2g073500 42.593 324 158 9 53 365 29 335 2.10e-84 260
MS.gene84006.t1 MTR_4g079700 40.187 321 170 7 53 365 47 353 1.95e-83 258
MS.gene84006.t1 MTR_4g088030 39.441 322 178 5 50 365 30 340 2.59e-83 257
MS.gene84006.t1 MTR_5g034810 40.303 330 176 8 44 365 26 342 1.99e-82 255
MS.gene84006.t1 MTR_4g126950 34.771 371 215 6 21 365 67 436 1.46e-81 256
MS.gene84006.t1 MTR_4g016940 39.820 334 182 8 51 365 84 417 2.08e-81 255
MS.gene84006.t1 MTR_5g059400 36.043 369 210 8 23 366 139 506 2.94e-81 258
MS.gene84006.t1 MTR_1g116130 36.043 369 210 8 23 366 139 506 2.94e-81 258
MS.gene84006.t1 MTR_4g087730 39.692 325 179 5 47 365 39 352 1.27e-80 251
MS.gene84006.t1 MTR_3g021090 39.506 324 177 5 49 365 27 338 4.11e-80 251
MS.gene84006.t1 MTR_7g109550 37.082 329 185 6 53 366 137 458 4.71e-80 253
MS.gene84006.t1 MTR_5g033800 36.919 344 199 6 40 365 130 473 8.34e-80 253
MS.gene84006.t1 MTR_3g035830 39.198 324 178 5 49 365 27 338 1.63e-78 245
MS.gene84006.t1 MTR_8g463790 40.841 333 173 9 53 365 71 399 5.40e-78 246
MS.gene84006.t1 MTR_3g021060 37.963 324 182 5 49 365 42 353 1.46e-76 240
MS.gene84006.t1 MTR_4g121560 36.747 332 189 6 53 365 114 443 1.95e-76 243
MS.gene84006.t1 MTR_4g014440 36.364 341 194 9 48 365 57 397 2.78e-75 238
MS.gene84006.t1 MTR_1g058380 36.294 394 212 12 1 365 1 384 6.50e-75 238
MS.gene84006.t1 MTR_8g069790 37.273 330 191 5 52 365 177 506 9.45e-74 238
MS.gene84006.t1 MTR_1g111010 38.957 326 166 11 50 365 29 331 2.54e-73 231
MS.gene84006.t1 MTR_7g068920 39.104 335 185 7 50 365 77 411 2.99e-73 234
MS.gene84006.t1 MTR_3g070900 37.725 334 187 8 53 365 106 439 6.13e-73 234
MS.gene84006.t1 MTR_7g070060 35.777 341 196 9 49 366 60 400 3.58e-72 231
MS.gene84006.t1 MTR_5g082880 37.059 340 190 8 51 366 163 502 3.88e-70 228
MS.gene84006.t1 MTR_5g083260 35.119 336 199 7 49 365 79 414 5.70e-68 220
MS.gene84006.t1 MTR_3g071650 34.940 332 197 7 53 365 89 420 9.87e-68 220
MS.gene84006.t1 MTR_2g022560 35.312 337 188 7 53 365 97 427 3.96e-67 219
MS.gene84006.t1 MTR_4g014430 34.808 339 201 8 45 365 100 436 4.82e-66 216
MS.gene84006.t1 MTR_7g094750 35.030 334 194 6 53 365 91 422 8.81e-66 215
MS.gene84006.t1 MTR_3g464570 38.550 262 147 4 116 365 3 262 2.99e-62 201
MS.gene84006.t1 MTR_8g093530 33.038 339 200 8 44 366 80 407 8.89e-59 196
MS.gene84006.t1 MTR_3g090120 34.393 346 198 8 39 366 116 450 1.47e-58 197
MS.gene84006.t1 MTR_5g033800 37.450 251 144 4 40 277 130 380 7.13e-58 192
MS.gene84006.t1 MTR_7g070060 38.655 238 137 5 49 277 60 297 1.02e-55 185
MS.gene84006.t1 MTR_7g094750 35.714 238 140 3 53 277 91 328 9.89e-51 172
MS.gene84006.t1 MTR_7g070060 32.394 284 171 7 104 366 15 298 1.32e-48 166
MS.gene84006.t1 MTR_8g027900 27.762 353 223 10 40 365 198 545 4.17e-38 144
MS.gene84006.t1 MTR_6g061710 31.148 366 197 16 40 365 154 504 1.34e-37 142
MS.gene84006.t1 MTR_1g076690 31.232 349 193 14 53 365 223 560 2.38e-36 139
MS.gene84006.t1 MTR_5g069320 28.028 289 139 6 96 365 145 383 7.05e-36 134
MS.gene84006.t1 MTR_7g019120 29.683 347 203 13 49 365 75 410 2.42e-35 134
MS.gene84006.t1 MTR_5g035080 27.701 361 220 10 37 365 59 410 2.61e-35 134
MS.gene84006.t1 MTR_7g033230 27.941 340 218 7 51 365 262 599 2.38e-34 134
MS.gene84006.t1 MTR_4g092830 27.697 343 210 12 53 365 48 382 5.56e-34 130
MS.gene84006.t1 MTR_7g033230 27.941 340 218 7 51 365 332 669 6.66e-34 132
MS.gene84006.t1 MTR_8g093870 29.201 363 213 14 35 365 112 462 1.06e-33 130
MS.gene84006.t1 MTR_8g104970 28.530 347 209 11 51 366 118 456 3.63e-33 129
MS.gene84006.t1 MTR_3g109840 28.280 343 206 9 53 365 73 405 4.05e-33 128
MS.gene84006.t1 MTR_5g035090 28.969 359 203 15 44 365 74 417 1.92e-32 126
MS.gene84006.t1 MTR_8g099300 25.926 351 213 12 50 365 71 409 7.55e-32 124
MS.gene84006.t1 MTR_4g125060 30.086 349 205 16 45 365 124 461 2.10e-31 124
MS.gene84006.t1 MTR_4g125060 30.086 349 205 16 45 365 163 500 3.03e-31 124
MS.gene84006.t1 MTR_4g125060 30.086 349 205 16 45 365 192 529 4.20e-31 124
MS.gene84006.t1 MTR_2g016060 28.693 352 188 13 57 365 59 390 4.35e-31 122
MS.gene84006.t1 MTR_5g035150 29.395 347 211 12 48 365 80 421 2.89e-30 120
MS.gene84006.t1 MTR_6g016880 27.168 346 213 9 49 365 74 409 2.13e-29 118
MS.gene84006.t1 MTR_6g016875 27.874 348 219 10 45 365 56 398 5.32e-29 116
MS.gene84006.t1 MTR_6g016240 27.978 361 211 15 41 365 59 406 1.05e-27 113
MS.gene84006.t1 MTR_5g035070 30.278 360 203 15 42 365 76 423 3.84e-27 111
MS.gene84006.t1 MTR_6g007777 27.966 354 198 16 51 366 114 448 1.59e-25 107
MS.gene84006.t1 MTR_7g019140 45.833 96 51 1 49 143 75 170 2.21e-23 95.9
MS.gene84006.t1 MTR_5g035120 33.813 139 87 2 9 142 27 165 2.00e-20 87.8
MS.gene84006.t1 MTR_3g021150 37.037 108 62 1 238 339 1 108 7.92e-18 79.0
MS.gene84006.t1 MTR_2g022580 32.773 119 68 2 112 218 2 120 2.65e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84006.t1 AT3G54260 62.264 371 129 6 1 366 1 365 4.20e-164 464
MS.gene84006.t1 AT2G31110 39.612 361 199 6 13 365 3 352 7.16e-90 275
MS.gene84006.t1 AT5G58600 39.403 335 186 6 44 365 60 390 4.66e-87 269
MS.gene84006.t1 AT2G42570 39.528 339 182 6 39 365 27 354 3.03e-86 265
MS.gene84006.t1 AT5G06700 40.000 335 184 6 48 365 250 584 4.15e-86 272
MS.gene84006.t1 AT1G29050 40.439 319 173 4 53 365 61 368 9.86e-86 265
MS.gene84006.t1 AT2G30900 41.718 326 168 8 51 365 41 355 1.24e-85 264
MS.gene84006.t1 AT2G34070 38.226 327 184 5 46 365 58 373 1.57e-83 259
MS.gene84006.t1 AT3G12060 39.403 335 186 5 48 365 191 525 2.86e-83 263
MS.gene84006.t1 AT5G20590 39.039 333 185 6 51 366 139 470 3.59e-82 259
MS.gene84006.t1 AT5G01360 38.393 336 185 8 51 365 34 368 2.25e-81 253
MS.gene84006.t1 AT5G01360 38.393 336 185 8 51 365 90 424 7.93e-81 254
MS.gene84006.t1 AT3G62390 36.043 369 201 8 23 365 102 461 1.18e-80 254
MS.gene84006.t1 AT5G49340 39.157 332 183 7 53 365 100 431 6.70e-80 252
MS.gene84006.t1 AT2G30010 37.778 360 191 8 35 365 31 386 1.36e-78 247
MS.gene84006.t1 AT2G30010 37.778 360 191 8 35 365 74 429 3.54e-78 247
MS.gene84006.t1 AT1G73140 39.458 332 179 9 53 365 67 395 1.82e-76 242
MS.gene84006.t1 AT2G40160 37.685 337 186 9 53 365 77 413 6.10e-76 241
MS.gene84006.t1 AT1G60790 36.257 342 194 9 46 365 183 522 7.84e-76 244
MS.gene84006.t1 AT2G40160 37.685 337 186 9 53 365 77 413 1.08e-75 241
MS.gene84006.t1 AT1G78710 34.615 338 200 7 38 365 20 346 1.16e-75 238
MS.gene84006.t1 AT3G14850 37.538 325 187 5 48 365 29 344 3.47e-75 236
MS.gene84006.t1 AT3G55990 37.202 336 190 7 51 365 138 473 2.24e-74 238
MS.gene84006.t1 AT2G40150 36.657 341 189 7 52 365 70 410 2.73e-74 236
MS.gene84006.t1 AT2G38320 32.861 353 214 9 37 366 43 395 6.21e-71 227
MS.gene84006.t1 AT5G01620 35.224 335 196 8 51 366 108 440 4.72e-70 226
MS.gene84006.t1 AT5G01620 35.224 335 196 8 51 366 108 440 4.72e-70 226
MS.gene84006.t1 AT5G01620 35.224 335 196 8 51 366 116 448 5.48e-70 226
MS.gene84006.t1 AT5G19160 35.556 360 200 8 32 365 81 434 1.76e-69 225
MS.gene84006.t1 AT3G11570 35.233 386 209 12 8 365 37 409 1.52e-68 222
MS.gene84006.t1 AT2G31110 40.230 261 151 4 13 270 3 261 3.68e-67 213
MS.gene84006.t1 AT3G11030 35.417 336 191 8 53 365 106 438 4.72e-66 216
MS.gene84006.t1 AT5G06230 37.464 347 176 10 50 365 18 354 6.50e-66 213
MS.gene84006.t1 AT5G06230 37.464 347 176 10 50 365 59 395 1.23e-65 214
MS.gene84006.t1 AT2G40320 33.924 395 215 13 8 365 27 412 1.31e-65 214
MS.gene84006.t1 AT3G06080 36.070 341 192 7 48 365 98 435 1.49e-65 215
MS.gene84006.t1 AT2G30900 43.043 230 126 5 51 277 41 268 1.99e-64 206
MS.gene84006.t1 AT5G58600 41.102 236 133 4 44 277 60 291 1.79e-63 204
MS.gene84006.t1 AT1G48880 34.743 331 184 9 53 366 115 430 6.01e-63 207
MS.gene84006.t1 AT3G62390 36.765 272 155 4 23 277 102 373 6.73e-62 204
MS.gene84006.t1 AT2G38320 32.372 312 190 7 76 366 1 312 2.98e-61 200
MS.gene84006.t1 AT2G40160 41.102 236 128 6 53 277 77 312 3.51e-60 196
MS.gene84006.t1 AT2G34070 36.800 250 141 4 122 365 1 239 2.61e-56 184
MS.gene84006.t1 AT5G20590 39.496 238 131 4 53 277 141 378 2.86e-56 188
MS.gene84006.t1 AT5G19160 36.680 259 148 4 32 277 81 336 6.24e-55 184
MS.gene84006.t1 AT1G78710 30.240 334 169 9 37 365 12 286 3.72e-50 170
MS.gene84006.t1 AT5G06230 38.057 247 134 4 50 277 59 305 7.47e-50 170
MS.gene84006.t1 AT3G06080 35.391 243 144 3 48 277 98 340 5.62e-49 168
MS.gene84006.t1 AT2G31110 40.580 207 107 4 164 365 9 204 1.65e-46 158
MS.gene84006.t1 AT3G14850 35.600 250 146 4 122 365 1 241 3.79e-46 158
MS.gene84006.t1 AT1G70230 31.977 344 182 14 53 365 79 401 8.05e-39 143
MS.gene84006.t1 AT1G01430 28.775 351 214 11 46 365 92 437 3.80e-38 142
MS.gene84006.t1 AT4G25360 30.812 357 206 14 43 365 165 514 9.54e-37 139
MS.gene84006.t1 AT4G25360 30.812 357 206 14 43 365 165 514 9.54e-37 139
MS.gene84006.t1 AT4G01080 27.415 383 243 12 8 365 48 420 1.13e-36 138
MS.gene84006.t1 AT4G11090 28.922 408 231 17 2 365 23 415 6.30e-36 135
MS.gene84006.t1 AT4G23790 28.451 355 194 12 51 362 77 414 1.84e-34 132
MS.gene84006.t1 AT5G51640 29.143 350 204 12 52 365 137 478 4.25e-34 132
MS.gene84006.t1 AT5G64020 28.448 348 208 15 53 365 57 398 9.77e-34 129
MS.gene84006.t1 AT5G64470 27.011 348 221 12 43 365 45 384 4.18e-33 127
MS.gene84006.t1 AT5G15890 29.480 346 197 11 47 362 181 509 9.04e-33 128
MS.gene84006.t1 AT5G64470 26.836 354 220 12 43 365 45 390 1.30e-32 126
MS.gene84006.t1 AT5G15900 28.612 353 218 11 40 365 54 399 5.16e-32 125
MS.gene84006.t1 AT5G15900 28.612 353 218 11 40 365 73 418 5.32e-32 125
MS.gene84006.t1 AT2G37720 27.907 344 207 14 51 365 141 472 5.30e-31 123
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 92 447 7.47e-31 122
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 186 541 1.44e-30 122
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 186 541 1.44e-30 122
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 186 541 1.44e-30 122
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 186 541 1.44e-30 122
MS.gene84006.t1 AT5G20680 26.400 375 222 17 26 365 168 523 1.50e-30 122
MS.gene84006.t1 AT3G02440 27.485 342 209 12 53 365 127 458 2.20e-29 118
MS.gene84006.t1 AT3G02440 27.485 342 209 12 53 365 135 466 2.75e-29 118
MS.gene84006.t1 AT2G14530 27.066 351 208 13 50 365 59 396 6.36e-28 113
MS.gene84006.t1 AT3G28150 27.387 398 221 19 8 365 31 400 7.41e-28 113
MS.gene84006.t1 AT5G64470 26.848 257 169 7 43 282 45 299 6.36e-24 100
MS.gene84006.t1 AT5G64470 26.953 256 168 7 43 281 45 298 9.75e-24 100

Find 86 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATCAATTGAGTTTCTT+TGG 0.205327 1.3:-32060942 MS.gene84006:CDS
CTATTCTTTGTTTCTCATCT+TGG 0.240185 1.3:+32059834 None:intergenic
TGCTAGAATCTCTATTGTTT+TGG 0.241013 1.3:+32062672 None:intergenic
TAATACCAACTGGTAGAAAA+AGG 0.249328 1.3:-32061086 MS.gene84006:CDS
CTTATGGTTGCTTATCAAAA+AGG 0.263528 1.3:-32060379 MS.gene84006:CDS
TTTGATGCACTAAGGTTTCT+TGG 0.268531 1.3:-32061195 MS.gene84006:CDS
TCTGAAGAAAATGAGGTTTC+CGG 0.283468 1.3:-32059933 MS.gene84006:CDS
TGGACAATGTTAACATAATC+AGG 0.291616 1.3:+32062692 None:intergenic
ATTCCTCTGATTGCAGTCAT+TGG 0.349039 1.3:-32059808 MS.gene84006:CDS
AACATCTCATTCTAAATATC+AGG 0.351041 1.3:+32059770 None:intergenic
GTTCAGGAGCATGTTGTTGC+AGG 0.359962 1.3:+32059978 None:intergenic
GACATCAATTGAGTTTCTTT+GGG 0.362068 1.3:-32060941 MS.gene84006:CDS
GCATGTCACCTAGGCATAAC+AGG 0.386311 1.3:-32060288 MS.gene84006:intron
CACTTCTCATAATCAGAATC+TGG 0.390203 1.3:+32062539 None:intergenic
TCTTTGTTTCTCATCTTGGC+TGG 0.397185 1.3:+32059838 None:intergenic
CATGCTTCTGAAAATGACTT+TGG 0.398420 1.3:+32060306 None:intergenic
AAATAAGAGAGTCTTACATT+TGG 0.399385 1.3:-32060884 MS.gene84006:CDS
ATTGAATGGAAGGCCATTGC+AGG 0.414061 1.3:+32061048 None:intergenic
TGCAGTCATTGGTGCCTCCC+TGG 0.417455 1.3:-32059797 MS.gene84006:CDS
TCTTTGGGCACCACTGTTAG+TGG 0.421618 1.3:-32060926 MS.gene84006:CDS
TAGACCCTCAAGATGATGTT+GGG 0.428304 1.3:-32062727 MS.gene84006:CDS
CTTACATTTGGATTTGATTG+AGG 0.428799 1.3:-32060872 MS.gene84006:CDS
ATGTCTTCATTACCATTGAA+TGG 0.434469 1.3:+32061034 None:intergenic
AAAGAAAGAAAATGAGTCAT+TGG 0.436084 1.3:+32062791 None:intergenic
TCATTGTCGTAATATCTTGC+AGG 0.436722 1.3:+32059906 None:intergenic
TTCTAAATATCAGGCACACC+AGG 0.447736 1.3:+32059779 None:intergenic
TTGAGGAGAATGCAAAGTAT+TGG 0.448978 1.3:-32060855 MS.gene84006:CDS
GCAGTTACTTGTCAAAAGAA+TGG 0.450896 1.3:-32062566 MS.gene84006:CDS
TGGAGAACAACTAAAGGTTC+AGG 0.465074 1.3:+32059962 None:intergenic
GTAACTGCAGTACTAAGATA+AGG 0.467342 1.3:+32062581 None:intergenic
ATGGACATCCATCAGTGTAT+AGG 0.472661 1.3:-32059868 MS.gene84006:CDS
CAAGATGAGAAACAAAGAAT+AGG 0.477046 1.3:-32059833 MS.gene84006:CDS
TGCAATGGCCTTCCATTCAA+TGG 0.483402 1.3:-32061046 MS.gene84006:intron
GTACTAAGATAAGGGCAGTT+AGG 0.483942 1.3:+32062590 None:intergenic
TCTGTAGGTTTGATGCACTA+AGG 0.486234 1.3:-32061203 MS.gene84006:intron
CAGTTACTTGTCAAAAGAAT+GGG 0.497842 1.3:-32062565 MS.gene84006:CDS
GTACAAGGTGTAATACCAAC+TGG 0.502784 1.3:-32061096 MS.gene84006:CDS
CTAGACCCTCAAGATGATGT+TGG 0.508079 1.3:-32062728 MS.gene84006:CDS
TAACTGCAGTACTAAGATAA+GGG 0.511515 1.3:+32062582 None:intergenic
CTGATTATGAGAAGTGGAAG+TGG 0.515175 1.3:-32062532 MS.gene84006:CDS
GTAATATCTTGCAGGTATAC+CGG 0.515454 1.3:+32059914 None:intergenic
GAGAAGGAAGAGAATAATGT+TGG 0.521562 1.3:-32061166 MS.gene84006:CDS
AAGGAGTTCTGAAGAAAATG+AGG 0.522264 1.3:-32059940 MS.gene84006:CDS
CTTCATTACCATTGAATGGA+AGG 0.525435 1.3:+32061038 None:intergenic
TATTTGATTCAGCTCAGTGG+TGG 0.527529 1.3:-32060813 MS.gene84006:CDS
TTCAATAATGAGAAATCAGT+GGG 0.529697 1.3:-32061136 MS.gene84006:CDS
GAAACCCAACATCATCTTGA+GGG 0.532117 1.3:+32062723 None:intergenic
ACTCCTTGGAGAACAACTAA+AGG 0.539662 1.3:+32059956 None:intergenic
CAAGTGGTTGTTCTATTCCA+AGG 0.549525 1.3:-32062505 MS.gene84006:intron
ACTGAGTACATGGGCAAGGT+GGG 0.549939 1.3:-32060356 MS.gene84006:CDS
GTTGCCTTCCTATACACTGA+TGG 0.558780 1.3:+32059860 None:intergenic
GGGCAGTTAGGATCATAAAG+AGG 0.560599 1.3:+32062602 None:intergenic
GGAAGGCCATTGCAGGTCCA+TGG 0.570480 1.3:+32061055 None:intergenic
GGCAGTTAGGATCATAAAGA+GGG 0.572794 1.3:+32062603 None:intergenic
GACCTACCATGGACCTGCAA+TGG 0.573638 1.3:-32061061 MS.gene84006:CDS
GAGTACATGGGCAAGGTGGG+TGG 0.582998 1.3:-32060353 MS.gene84006:CDS
TCTTTAGTATGCTTAGTACA+AGG 0.583313 1.3:-32061111 MS.gene84006:CDS
GAGAATGCAAAGTATTGGAG+AGG 0.588312 1.3:-32060850 MS.gene84006:CDS
ATGACTGCCTTACGCAGAGA+TGG 0.588976 1.3:-32059887 MS.gene84006:CDS
ATATAGTACCTGTTATGCCT+AGG 0.589873 1.3:+32060280 None:intergenic
AGGAAGAGAATAATGTTGGT+GGG 0.590257 1.3:-32061162 MS.gene84006:CDS
GACTGAGTACATGGGCAAGG+TGG 0.591699 1.3:-32060357 MS.gene84006:CDS
ATGAGTCATTGGTGTTGCCA+TGG 0.591707 1.3:+32062802 None:intergenic
TTGTATTTGATTCAGCTCAG+TGG 0.592724 1.3:-32060816 MS.gene84006:CDS
GAACCTTTAGTTGTTCTCCA+AGG 0.596967 1.3:-32059959 MS.gene84006:CDS
CAGATTCTGATTATGAGAAG+TGG 0.598360 1.3:-32062538 MS.gene84006:CDS
CTTGGAATAGAACAACCACT+TGG 0.601949 1.3:+32062506 None:intergenic
AATGACTGCAATCAGAGGAA+TGG 0.604666 1.3:+32059810 None:intergenic
TGGATGTCCATCTCTGCGTA+AGG 0.608878 1.3:+32059880 None:intergenic
GGCCATTGCAGGTCCATGGT+AGG 0.611844 1.3:+32061059 None:intergenic
AAGGAAGAGAATAATGTTGG+TGG 0.612533 1.3:-32061163 MS.gene84006:CDS
ATTCAATAATGAGAAATCAG+TGG 0.617219 1.3:-32061137 MS.gene84006:CDS
AGAAACCCAACATCATCTTG+AGG 0.617280 1.3:+32062722 None:intergenic
TTCTTCAGTTCCACTAACAG+TGG 0.620275 1.3:+32060916 None:intergenic
CTAGAAGAGATAATGTGCAG+TGG 0.625214 1.3:+32062747 None:intergenic
GCACCAATGACTGCAATCAG+AGG 0.627161 1.3:+32059805 None:intergenic
ATACAGGTGGGATTACTACA+AGG 0.634100 1.3:-32060431 MS.gene84006:intron
ACTGAGCTGAATCAAATACA+AGG 0.634640 1.3:+32060818 None:intergenic
TCTAAATATCAGGCACACCA+GGG 0.646619 1.3:+32059780 None:intergenic
AGGTGGGATTACTACAAGGA+GGG 0.648410 1.3:-32060427 MS.gene84006:CDS
AAGGACTGAGTACATGGGCA+AGG 0.650632 1.3:-32060360 MS.gene84006:CDS
TTACTTGTCAAAAGAATGGG+AGG 0.658568 1.3:-32062562 MS.gene84006:CDS
ACATCCATCAGTGTATAGGA+AGG 0.659861 1.3:-32059864 MS.gene84006:CDS
CAGGTGGGATTACTACAAGG+AGG 0.703135 1.3:-32060428 MS.gene84006:CDS
AAATATCAGGCACACCAGGG+AGG 0.749580 1.3:+32059783 None:intergenic
AAGTGGAAGTGGAAGCCAAG+TGG 0.762111 1.3:-32062521 MS.gene84006:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAATATAAATTTTATCTA+AGG - chr1.3:32060264-32060283 MS.gene84006:intron 10.0%
!!! CTTAGATAAAATTTATATTT+TGG + chr1.3:32060266-32060285 None:intergenic 10.0%
!! ACTCAAATTAATTAACTAAA+AGG + chr1.3:32060619-32060638 None:intergenic 15.0%
!! AGATTTATTATTCAAATTTG+TGG - chr1.3:32060724-32060743 MS.gene84006:intron 15.0%
!! CATTTACTTAAATACAAATT+TGG + chr1.3:32061300-32061319 None:intergenic 15.0%
!!! AGTATATTCTAGATTTTATA+GGG - chr1.3:32060666-32060685 MS.gene84006:intron 15.0%
!!! CTCAAATTTTAATTTAATGA+TGG + chr1.3:32060204-32060223 None:intergenic 15.0%
!!! GAATATATTCTAGATTTTTT+AGG - chr1.3:32060689-32060708 MS.gene84006:intron 15.0%
!!! TAGTATATTCTAGATTTTAT+AGG - chr1.3:32060665-32060684 MS.gene84006:intron 15.0%
!! AAACTATCTTAGAAAACTTA+TGG - chr1.3:32062010-32062029 MS.gene84006:intron 20.0%
!! AACTATCTTAGAAAACTTAT+GGG - chr1.3:32062011-32062030 MS.gene84006:intron 20.0%
!! AGTCCTTAAAATAAGTAAAA+TGG + chr1.3:32061217-32061236 None:intergenic 20.0%
!! ATAAAACCACAAATATATTC+AGG + chr1.3:32061141-32061160 None:intergenic 20.0%
!! CTTAAAATAAGTAAAATGGA+AGG + chr1.3:32061213-32061232 None:intergenic 20.0%
!! TGATTTCCGATAAAATATTA+TGG + chr1.3:32062426-32062445 None:intergenic 20.0%
!! TTTATCAATTAAATGAATGC+AGG + chr1.3:32060367-32060386 None:intergenic 20.0%
!!! AAAATGACTTTGGTTTTTTT+AGG + chr1.3:32062268-32062287 None:intergenic 20.0%
!!! AAATGACTTTGGTTTTTTTA+GGG + chr1.3:32062267-32062286 None:intergenic 20.0%
!!! ATTGAGTTCTAAAAAAAACA+TGG - chr1.3:32060588-32060607 MS.gene84006:intron 20.0%
!!! CTTCCATTTTACTTATTTTA+AGG - chr1.3:32061211-32061230 MS.gene84006:intron 20.0%
!!! GTATATTCTAGATTTTATAG+GGG - chr1.3:32060667-32060686 MS.gene84006:intron 20.0%
!!! TATTGTAATTTGTACAGATA+TGG - chr1.3:32060907-32060926 MS.gene84006:CDS 20.0%
!!! TTTGATAAACTTTCTAAAAG+AGG - chr1.3:32062073-32062092 MS.gene84006:intron 20.0%
!!! TTTGTGGTTTTATTTTAATG+TGG - chr1.3:32061148-32061167 MS.gene84006:CDS 20.0%
!!! TTTTTTTAGAACTCAATATC+CGG + chr1.3:32060585-32060604 None:intergenic 20.0%
! AAAATAAGTAAAATGGAAGG+TGG + chr1.3:32061210-32061229 None:intergenic 25.0%
! AAAGAAAGAAAATGAGTCAT+TGG + chr1.3:32059793-32059812 None:intergenic 25.0%
! AAATAAGAGAGTCTTACATT+TGG - chr1.3:32061697-32061716 MS.gene84006:intron 25.0%
! AAATTATGCATATAGGAGAT+GGG + chr1.3:32060866-32060885 None:intergenic 25.0%
! AATGCCAAAAACAAAACTAT+AGG + chr1.3:32062499-32062518 None:intergenic 25.0%
! ACAAATATATTCAGGCAAAA+TGG + chr1.3:32061133-32061152 None:intergenic 25.0%
! ATCCCTAAAATTGTAAATGT+TGG - chr1.3:32060977-32060996 MS.gene84006:intron 25.0%
! ATTCAATAATGAGAAATCAG+TGG - chr1.3:32061444-32061463 MS.gene84006:intron 25.0%
! GTAAATGAATTTCAACACTT+TGG - chr1.3:32061313-32061332 MS.gene84006:intron 25.0%
! GTGTATAGAAGAAAACAAAA+AGG + chr1.3:32060118-32060137 None:intergenic 25.0%
! GTTCTTGAAATTGTAAATGT+CGG + chr1.3:32061278-32061297 None:intergenic 25.0%
! TATAGAAGAAAACAAAAAGG+TGG + chr1.3:32060115-32060134 None:intergenic 25.0%
! TTCAATAATGAGAAATCAGT+GGG - chr1.3:32061445-32061464 MS.gene84006:intron 25.0%
!! ACGACACCATAATATTTTAT+CGG - chr1.3:32062417-32062436 MS.gene84006:intron 25.0%
!! ATTTTGCCTGAATATATTTG+TGG - chr1.3:32061132-32061151 MS.gene84006:CDS 25.0%
!! CAAGAAAGAAGCATATAAAT+GGG + chr1.3:32062126-32062145 None:intergenic 25.0%
!! GACAAAGATAAACAAGTTTT+AGG + chr1.3:32062340-32062359 None:intergenic 25.0%
!! GACCAACATTTACAATTTTA+GGG + chr1.3:32060982-32061001 None:intergenic 25.0%
!! GTGGTGTCAATAATTTATAA+AGG + chr1.3:32061597-32061616 None:intergenic 25.0%
!! TCAAGAAAGAAGCATATAAA+TGG + chr1.3:32062127-32062146 None:intergenic 25.0%
!! TGACCAACATTTACAATTTT+AGG + chr1.3:32060983-32061002 None:intergenic 25.0%
!!! AGTTCTAAAAAAAACATGGT+TGG - chr1.3:32060592-32060611 MS.gene84006:intron 25.0%
!!! GATTTTTTATGTAGGGAAAA+TGG - chr1.3:32062538-32062557 MS.gene84006:CDS 25.0%
AACTAGTTAAGTTTACAAGC+AGG + chr1.3:32061351-32061370 None:intergenic 30.0%
AATAAGCCAAAATCAGCTTA+TGG - chr1.3:32062034-32062053 MS.gene84006:intron 30.0%
AATACCAACTGGTAGAAAAA+GGG - chr1.3:32061496-32061515 MS.gene84006:intron 30.0%
AATGGAATGATATGAAGAGA+AGG + chr1.3:32061849-32061868 None:intergenic 30.0%
ACTGTGGAAATTATGCATAT+AGG + chr1.3:32060873-32060892 None:intergenic 30.0%
ACTTAGTGAGATATAAGACT+TGG + chr1.3:32062376-32062395 None:intergenic 30.0%
AGACATCAATTGAGTTTCTT+TGG - chr1.3:32061639-32061658 MS.gene84006:intron 30.0%
AGTCTCCAAATTTCATCAAT+AGG - chr1.3:32061007-32061026 MS.gene84006:intron 30.0%
AGTTGCCTATTGATGAAATT+TGG + chr1.3:32061015-32061034 None:intergenic 30.0%
ATGTCTTCATTACCATTGAA+TGG + chr1.3:32061550-32061569 None:intergenic 30.0%
CAAGATGAGAAACAAAGAAT+AGG - chr1.3:32062748-32062767 MS.gene84006:CDS 30.0%
CAGTTACTTGTCAAAAGAAT+GGG - chr1.3:32060016-32060035 MS.gene84006:CDS 30.0%
CTTACATTTGGATTTGATTG+AGG - chr1.3:32061709-32061728 MS.gene84006:intron 30.0%
CTTATGGTTGCTTATCAAAA+AGG - chr1.3:32062202-32062221 MS.gene84006:intron 30.0%
CTTCTAACAGAAAAACTTCA+GGG + chr1.3:32061074-32061093 None:intergenic 30.0%
GAAATTATGCATATAGGAGA+TGG + chr1.3:32060867-32060886 None:intergenic 30.0%
GACATCAATTGAGTTTCTTT+GGG - chr1.3:32061640-32061659 MS.gene84006:intron 30.0%
GATCAAAAACATGACTCTTA+TGG - chr1.3:32062186-32062205 MS.gene84006:intron 30.0%
GATCTCAAATCAACAACATT+AGG + chr1.3:32060413-32060432 None:intergenic 30.0%
GATTCCTTCTGTATCAATTT+CGG - chr1.3:32060465-32060484 MS.gene84006:intron 30.0%
GTTAAGATTTAGAAGCTCAA+TGG + chr1.3:32062467-32062486 None:intergenic 30.0%
TAACTGCAGTACTAAGATAA+GGG + chr1.3:32060002-32060021 None:intergenic 30.0%
TAAGAGGGAAATTGAGAAAA+TGG + chr1.3:32061867-32061886 None:intergenic 30.0%
TAATACCAACTGGTAGAAAA+AGG - chr1.3:32061495-32061514 MS.gene84006:intron 30.0%
TAGTATAACAACTTGTGTCA+AGG + chr1.3:32060647-32060666 None:intergenic 30.0%
TCTTCTAACAGAAAAACTTC+AGG + chr1.3:32061075-32061094 None:intergenic 30.0%
TCTTTAGTATGCTTAGTACA+AGG - chr1.3:32061470-32061489 MS.gene84006:intron 30.0%
TGGACAATGTTAACATAATC+AGG + chr1.3:32059892-32059911 None:intergenic 30.0%
! ACTAAGTGAAGTTGATTACA+TGG - chr1.3:32062389-32062408 MS.gene84006:intron 30.0%
! AGTTTTTATCAACTCTGAGT+AGG - chr1.3:32060780-32060799 MS.gene84006:intron 30.0%
! ATTAAGTAGTTTGTCCTAAG+AGG + chr1.3:32061883-32061902 None:intergenic 30.0%
! ATTTTCTCAATTTCCCTCTT+AGG - chr1.3:32061866-32061885 MS.gene84006:intron 30.0%
! CTATTCTTTGTTTCTCATCT+TGG + chr1.3:32062750-32062769 None:intergenic 30.0%
! GGATTAAAATCTGAGATCAA+TGG + chr1.3:32060845-32060864 None:intergenic 30.0%
! GTTTTTATCAACTCTGAGTA+GGG - chr1.3:32060781-32060800 MS.gene84006:intron 30.0%
! TAAAATCTGAGATCAATGGA+GGG + chr1.3:32060841-32060860 None:intergenic 30.0%
! TATTGACACCACAATTTTCT+CGG - chr1.3:32061605-32061624 MS.gene84006:intron 30.0%
! TGACTTTTCTGTTGGAAAAT+GGG - chr1.3:32059939-32059958 MS.gene84006:CDS 30.0%
! TGCTAGAATCTCTATTGTTT+TGG + chr1.3:32059912-32059931 None:intergenic 30.0%
! TTAAAATCTGAGATCAATGG+AGG + chr1.3:32060842-32060861 None:intergenic 30.0%
! TTAAGTAGTTTGTCCTAAGA+GGG + chr1.3:32061882-32061901 None:intergenic 30.0%
! TTTTATGTAGGGAAAATGGT+TGG - chr1.3:32062542-32062561 MS.gene84006:CDS 30.0%
! TTTTTATCAACTCTGAGTAG+GGG - chr1.3:32060782-32060801 MS.gene84006:intron 30.0%
!!! AGCGATTGGATTTTTTATGT+AGG - chr1.3:32062530-32062549 MS.gene84006:CDS 30.0%
!!! GCGATTGGATTTTTTATGTA+GGG - chr1.3:32062531-32062550 MS.gene84006:CDS 30.0%
!!! TGTAAGACTCTCTTATTTTC+TGG + chr1.3:32061695-32061714 None:intergenic 30.0%
!!! TTCGCCTATAGTTTTGTTTT+TGG - chr1.3:32062492-32062511 MS.gene84006:intron 30.0%
AAGGAAGAGAATAATGTTGG+TGG - chr1.3:32061418-32061437 MS.gene84006:intron 35.0%
AATCCTATCCGAGAAAATTG+TGG + chr1.3:32061616-32061635 None:intergenic 35.0%
ACTGAGCTGAATCAAATACA+AGG + chr1.3:32061766-32061785 None:intergenic 35.0%
AGGAAGAGAATAATGTTGGT+GGG - chr1.3:32061419-32061438 MS.gene84006:intron 35.0%
ATATAGTACCTGTTATGCCT+AGG + chr1.3:32062304-32062323 None:intergenic 35.0%
ATCAAAAAGGACTGAGTACA+TGG - chr1.3:32062215-32062234 MS.gene84006:intron 35.0%
ATGCATAATTTCCACAGTCA+TGG - chr1.3:32060875-32060894 MS.gene84006:CDS 35.0%
ATGTTAACCTGTACTCTTAG+TGG - chr1.3:32060226-32060245 MS.gene84006:intron 35.0%
ATTTCATTGTCTCTAGCGAT+TGG - chr1.3:32062516-32062535 MS.gene84006:CDS 35.0%
CAAAATCAGCTTATGGACAT+GGG - chr1.3:32062041-32062060 MS.gene84006:intron 35.0%
CACTTCTCATAATCAGAATC+TGG + chr1.3:32060045-32060064 None:intergenic 35.0%
CAGATTCTGATTATGAGAAG+TGG - chr1.3:32060043-32060062 MS.gene84006:intron 35.0%
CTTCATTACCATTGAATGGA+AGG + chr1.3:32061546-32061565 None:intergenic 35.0%
CTTTCTTGACTAATACAGGT+GGG - chr1.3:32062138-32062157 MS.gene84006:intron 35.0%
GAGAAGGAAGAGAATAATGT+TGG - chr1.3:32061415-32061434 MS.gene84006:intron 35.0%
GCAGTTACTTGTCAAAAGAA+TGG - chr1.3:32060015-32060034 MS.gene84006:CDS 35.0%
GCTTCTTTCTTGACTAATAC+AGG - chr1.3:32062134-32062153 MS.gene84006:intron 35.0%
GGTTTCTTGGAAAAATGAGA+AGG - chr1.3:32061399-32061418 MS.gene84006:intron 35.0%
GTAACTGCAGTACTAAGATA+AGG + chr1.3:32060003-32060022 None:intergenic 35.0%
GTAATATCTTGCAGGTATAC+CGG + chr1.3:32062670-32062689 None:intergenic 35.0%
TACAGAAGGAATCGAACATA+AGG + chr1.3:32060458-32060477 None:intergenic 35.0%
TCAAAAAGGACTGAGTACAT+GGG - chr1.3:32062216-32062235 MS.gene84006:intron 35.0%
TCATTGTCGTAATATCTTGC+AGG + chr1.3:32062678-32062697 None:intergenic 35.0%
TCTGAAGAAAATGAGGTTTC+CGG - chr1.3:32062648-32062667 MS.gene84006:CDS 35.0%
TCTGATAAAACAAGCTCGTA+AGG - chr1.3:32061797-32061816 MS.gene84006:intron 35.0%
TCTTTCTTGACTAATACAGG+TGG - chr1.3:32062137-32062156 MS.gene84006:intron 35.0%
TGACTGACATGTTACAAACT+AGG + chr1.3:32061044-32061063 None:intergenic 35.0%
TTACTTGTCAAAAGAATGGG+AGG - chr1.3:32060019-32060038 MS.gene84006:CDS 35.0%
TTGAGGAGAATGCAAAGTAT+TGG - chr1.3:32061726-32061745 MS.gene84006:intron 35.0%
TTGTATTTGATTCAGCTCAG+TGG - chr1.3:32061765-32061784 MS.gene84006:intron 35.0%
TTTGATGCACTAAGGTTTCT+TGG - chr1.3:32061386-32061405 MS.gene84006:intron 35.0%
! AACTAGTTTATCACCTCTGT+AGG - chr1.3:32061363-32061382 MS.gene84006:intron 35.0%
! AAGAGATGTGACTTTTCTGT+TGG - chr1.3:32059931-32059950 MS.gene84006:CDS 35.0%
! CATGCTTCTGAAAATGACTT+TGG + chr1.3:32062278-32062297 None:intergenic 35.0%
! CTTAGAGTGTGCTTTTCTTA+AGG - chr1.3:32060432-32060451 MS.gene84006:intron 35.0%
! GTGACTTTTCTGTTGGAAAA+TGG - chr1.3:32059938-32059957 MS.gene84006:CDS 35.0%
! TCATTTTCTTCAGAACTCCT+TGG + chr1.3:32062642-32062661 None:intergenic 35.0%
!! AAGGAGTTCTGAAGAAAATG+AGG - chr1.3:32062641-32062660 MS.gene84006:CDS 35.0%
AATGACTGCAATCAGAGGAA+TGG + chr1.3:32062774-32062793 None:intergenic 40.0%
ACATCCATCAGTGTATAGGA+AGG - chr1.3:32062717-32062736 MS.gene84006:CDS 40.0%
ACTCCTTGGAGAACAACTAA+AGG + chr1.3:32062628-32062647 None:intergenic 40.0%
AGAAACCCAACATCATCTTG+AGG + chr1.3:32059862-32059881 None:intergenic 40.0%
AGATTTAGAAGCTCAATGGC+TGG + chr1.3:32062463-32062482 None:intergenic 40.0%
ATACAGGTGGGATTACTACA+AGG - chr1.3:32062150-32062169 MS.gene84006:intron 40.0%
ATGGACATCCATCAGTGTAT+AGG - chr1.3:32062713-32062732 MS.gene84006:CDS 40.0%
ATTCCTCTGATTGCAGTCAT+TGG - chr1.3:32062773-32062792 MS.gene84006:CDS 40.0%
CAAGTGGTTGTTCTATTCCA+AGG - chr1.3:32060076-32060095 MS.gene84006:intron 40.0%
CCAAAATCAGCTTATGGACA+TGG - chr1.3:32062040-32062059 MS.gene84006:intron 40.0%
CCAATCTCATTGTTCACTTG+CGG + chr1.3:32060537-32060556 None:intergenic 40.0%
CTAGAAGAGATAATGTGCAG+TGG + chr1.3:32059837-32059856 None:intergenic 40.0%
CTGATTATGAGAAGTGGAAG+TGG - chr1.3:32060049-32060068 MS.gene84006:intron 40.0%
CTTGGAATAGAACAACCACT+TGG + chr1.3:32060078-32060097 None:intergenic 40.0%
GAAACCCAACATCATCTTGA+GGG + chr1.3:32059861-32059880 None:intergenic 40.0%
GAACCTTTAGTTGTTCTCCA+AGG - chr1.3:32062622-32062641 MS.gene84006:CDS 40.0%
GGAATGATATGAAGAGAAGG+TGG + chr1.3:32061846-32061865 None:intergenic 40.0%
GGCAGTTAGGATCATAAAGA+GGG + chr1.3:32059981-32060000 None:intergenic 40.0%
GTACAAGGTGTAATACCAAC+TGG - chr1.3:32061485-32061504 MS.gene84006:intron 40.0%
GTACAGATATGGTAAGCTTG+TGG - chr1.3:32060918-32060937 MS.gene84006:CDS 40.0%
GTACTAAGATAAGGGCAGTT+AGG + chr1.3:32059994-32060013 None:intergenic 40.0%
GTGGATGATGAGTTTGTGAA+TGG - chr1.3:32060937-32060956 MS.gene84006:CDS 40.0%
TAACACAAAAGCCATGACTG+TGG + chr1.3:32060889-32060908 None:intergenic 40.0%
TAGACCCTCAAGATGATGTT+GGG - chr1.3:32059854-32059873 MS.gene84006:CDS 40.0%
TATTTGATTCAGCTCAGTGG+TGG - chr1.3:32061768-32061787 MS.gene84006:intron 40.0%
TCCACCGAAATTGATACAGA+AGG + chr1.3:32060472-32060491 None:intergenic 40.0%
TCCTTCTGTATCAATTTCGG+TGG - chr1.3:32060468-32060487 MS.gene84006:intron 40.0%
TCTGTAGGTTTGATGCACTA+AGG - chr1.3:32061378-32061397 MS.gene84006:intron 40.0%
TGGAGAACAACTAAAGGTTC+AGG + chr1.3:32062622-32062641 None:intergenic 40.0%
TGTTGTTGCAGGCTGAAAAA+TGG + chr1.3:32062595-32062614 None:intergenic 40.0%
TTAGTGCATCAAACCTACAG+AGG + chr1.3:32061379-32061398 None:intergenic 40.0%
TTCTTCAGTTCCACTAACAG+TGG + chr1.3:32061668-32061687 None:intergenic 40.0%
! ACACCACAATTTTCTCGGAT+AGG - chr1.3:32061610-32061629 MS.gene84006:intron 40.0%
! ACCAAATCCACTAAGAGTAC+AGG + chr1.3:32060236-32060255 None:intergenic 40.0%
! CTCTCGATTAGTCCATCTTT+AGG - chr1.3:32060559-32060578 MS.gene84006:intron 40.0%
! TCTTTGTTTCTCATCTTGGC+TGG + chr1.3:32062746-32062765 None:intergenic 40.0%
!! ACCTGTACTCTTAGTGGATT+TGG - chr1.3:32060232-32060251 MS.gene84006:intron 40.0%
!! CCATGTCCATAAGCTGATTT+TGG + chr1.3:32062043-32062062 None:intergenic 40.0%
!! GAGAATGCAAAGTATTGGAG+AGG - chr1.3:32061731-32061750 MS.gene84006:intron 40.0%
!! TTTTCAGAAGCATGTCACCT+AGG - chr1.3:32062284-32062303 MS.gene84006:intron 40.0%
AGAAAAAGGGTGACCTACCA+TGG - chr1.3:32061509-32061528 MS.gene84006:intron 45.0%
AGGTGGGATTACTACAAGGA+GGG - chr1.3:32062154-32062173 MS.gene84006:intron 45.0%
ATCTCATTGTTCACTTGCGG+CGG + chr1.3:32060534-32060553 None:intergenic 45.0%
ATTGAATGGAAGGCCATTGC+AGG + chr1.3:32061536-32061555 None:intergenic 45.0%
CCGCAAGTGAACAATGAGAT+TGG - chr1.3:32060534-32060553 MS.gene84006:intron 45.0%
CTAGACCCTCAAGATGATGT+TGG - chr1.3:32059853-32059872 MS.gene84006:CDS 45.0%
CTCAATATCCGGCCTAAAGA+TGG + chr1.3:32060574-32060593 None:intergenic 45.0%
GCAAAATGGTCCTTGTGACT+TGG + chr1.3:32061119-32061138 None:intergenic 45.0%
GGGCAGTTAGGATCATAAAG+AGG + chr1.3:32059982-32060001 None:intergenic 45.0%
GTTGCCTTCCTATACACTGA+TGG + chr1.3:32062724-32062743 None:intergenic 45.0%
TGCAATGGCCTTCCATTCAA+TGG - chr1.3:32061535-32061554 MS.gene84006:intron 45.0%
TGCAGGCTGAAAAATGGTAG+AGG + chr1.3:32062589-32062608 None:intergenic 45.0%
! ATCTGAGATCAATGGAGGGT+TGG + chr1.3:32060837-32060856 None:intergenic 45.0%
! CGATTAGTCCATCTTTAGGC+CGG - chr1.3:32060563-32060582 MS.gene84006:intron 45.0%
! GTCACCCTTTTTCTACCAGT+TGG + chr1.3:32061503-32061522 None:intergenic 45.0%
! TAGAAGCTCAATGGCTGGTT+TGG + chr1.3:32062458-32062477 None:intergenic 45.0%
!! GTCCTTGTGACTTGGTAATG+CGG + chr1.3:32061111-32061130 None:intergenic 45.0%
AAATATCAGGCACACCAGGG+AGG + chr1.3:32062801-32062820 None:intergenic 50.0%
AAGGACTGAGTACATGGGCA+AGG - chr1.3:32062221-32062240 MS.gene84006:intron 50.0%
AAGTGGAAGTGGAAGCCAAG+TGG - chr1.3:32060060-32060079 MS.gene84006:intron 50.0%
ACTGAGTACATGGGCAAGGT+GGG - chr1.3:32062225-32062244 MS.gene84006:intron 50.0%
ATCAACTCTGAGTAGGGGAG+TGG - chr1.3:32060787-32060806 MS.gene84006:intron 50.0%
ATGACTGCCTTACGCAGAGA+TGG - chr1.3:32062694-32062713 MS.gene84006:CDS 50.0%
CAGGTGGGATTACTACAAGG+AGG - chr1.3:32062153-32062172 MS.gene84006:intron 50.0%
GCACCAATGACTGCAATCAG+AGG + chr1.3:32062779-32062798 None:intergenic 50.0%
GCATGTCACCTAGGCATAAC+AGG - chr1.3:32062293-32062312 MS.gene84006:intron 50.0%
GTGGCAATGTGAACAGACCT+TGG + chr1.3:32060096-32060115 None:intergenic 50.0%
TCTCATTGTTCACTTGCGGC+GGG + chr1.3:32060533-32060552 None:intergenic 50.0%
TCTTTGGGCACCACTGTTAG+TGG - chr1.3:32061655-32061674 MS.gene84006:intron 50.0%
TGAGTAGGGGAGTGGAATGT+GGG - chr1.3:32060795-32060814 MS.gene84006:CDS 50.0%
TGGATGTCCATCTCTGCGTA+AGG + chr1.3:32062704-32062723 None:intergenic 50.0%
! GTTCAGGAGCATGTTGTTGC+AGG + chr1.3:32062606-32062625 None:intergenic 50.0%
CGCCGCATTACCAAGTCACA+AGG - chr1.3:32061106-32061125 MS.gene84006:CDS 55.0%
CTGAGTAGGGGAGTGGAATG+TGG - chr1.3:32060794-32060813 MS.gene84006:CDS 55.0%
GACCTACCATGGACCTGCAA+TGG - chr1.3:32061520-32061539 MS.gene84006:intron 55.0%
GACTGAGTACATGGGCAAGG+TGG - chr1.3:32062224-32062243 MS.gene84006:intron 55.0%
GAGTACATGGGCAAGGTGGG+TGG - chr1.3:32062228-32062247 MS.gene84006:intron 60.0%
GGAAGGCCATTGCAGGTCCA+TGG + chr1.3:32061529-32061548 None:intergenic 60.0%
GGCCATTGCAGGTCCATGGT+AGG + chr1.3:32061525-32061544 None:intergenic 60.0%
! TGCAGTCATTGGTGCCTCCC+TGG - chr1.3:32062784-32062803 MS.gene84006:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 32059781 32062822 32059781 ID=MS.gene84006
chr1.3 mRNA 32059781 32062822 32059781 ID=MS.gene84006.t1;Parent=MS.gene84006
chr1.3 exon 32062506 32062822 32062506 ID=MS.gene84006.t1.exon1;Parent=MS.gene84006.t1
chr1.3 CDS 32062506 32062822 32062506 ID=cds.MS.gene84006.t1;Parent=MS.gene84006.t1
chr1.3 exon 32061047 32061218 32061047 ID=MS.gene84006.t1.exon2;Parent=MS.gene84006.t1
chr1.3 CDS 32061047 32061218 32061047 ID=cds.MS.gene84006.t1;Parent=MS.gene84006.t1
chr1.3 exon 32060790 32060971 32060790 ID=MS.gene84006.t1.exon3;Parent=MS.gene84006.t1
chr1.3 CDS 32060790 32060971 32060790 ID=cds.MS.gene84006.t1;Parent=MS.gene84006.t1
chr1.3 exon 32060289 32060447 32060289 ID=MS.gene84006.t1.exon4;Parent=MS.gene84006.t1
chr1.3 CDS 32060289 32060447 32060289 ID=cds.MS.gene84006.t1;Parent=MS.gene84006.t1
chr1.3 exon 32059781 32060051 32059781 ID=MS.gene84006.t1.exon5;Parent=MS.gene84006.t1
chr1.3 CDS 32059781 32060051 32059781 ID=cds.MS.gene84006.t1;Parent=MS.gene84006.t1
Gene Sequence

>MS.gene84006

ATGGCAACACCAATGACTCATTTTCTTTCTTTTTTCTTGTTGTTCAGCTTCTTCCACTGCACATTATCTCTTCTAGACCCTCAAGATGATGTTGGGTTTCTAGTTGAACCTGATTATGTTAACATTGTCCAAAACAATAGAGATTCTAGCAAGAGATGTGACTTTTCTGTTGGAAAATGGGTTTATGATGAATCTTACCCTCTTTATGATCCTAACTGCCCTTATCTTAGTACTGCAGTTACTTGTCAAAAGAATGGGAGGCCAGATTCTGATTATGAGAAGTGGAAGTGGAAGCCAAGTGGTTGTTCTATTCCAAGGTCTGTTCACATTGCCACCTTTTTGTTTTCTTCTATACACTCTCATTTACTCAATATTTATTTATTTATTTATCATTTTTTTTATATTTAGTATATATATGATCCATCATTAAATTAAAATTTGAGAAATGTTAACCTGTACTCTTAGTGGATTTGGTAATCAGTCCAAAATATAAATTTTATCTAAGGAATAATGCATTTAATAAAATAAAACTTTAAAAAACATTTTTTATTTTTTTTAGAGCACTTAAAATTTCAATAGTAGTCCTGCATTCATTTAATTGATAAACAAACTTTACTAATCAAACATGACCTAATGTTGTTGATTTGAGATCTTAGAGTGTGCTTTTCTTAAGGCCTTATGTTCGATTCCTTCTGTATCAATTTCGGTGGACTAGTTATATAGAGATTTGCTTTGACTTTTAAATGAAACCCGCCGCAAGTGAACAATGAGATTGGATCTCTCGATTAGTCCATCTTTAGGCCGGATATTGAGTTCTAAAAAAAACATGGTTGGACCTTTTAGTTAATTAATTTGAGTATTTACCTTGACACAAGTTGTTATACTAGTATATTCTAGATTTTATAGGGGAATATATTCTAGATTTTTTAGGTGAATATATTCTAGATTTATTATTCAAATTTGTGGCACTTTTGTCACTTTTTTATTTTTGTCAATGCTAGTTTTTATCAACTCTGAGTAGGGGAGTGGAATGTGGGATGTTACTATGTCATTCCAACCCTCCATTGATCTCAGATTTTAATCCCATCTCCTATATGCATAATTTCCACAGTCATGGCTTTTGTGTTATTGTAATTTGTACAGATATGGTAAGCTTGTGGATGATGAGTTTGTGAATGGAGTAGATGATCTAATTCATCCCTAAAATTGTAAATGTTGGTCATTTTAGTCTCCAAATTTCATCAATAGGCAACTACGTCTCCTAGTTTGTAACATGTCAGTCATTTTAGTCCCTGAAGTTTTTCTGTTAGAAGATATGAGCTAGACGCCGCATTACCAAGTCACAAGGACCATTTTGCCTGAATATATTTGTGGTTTTATTTTAATGTGGTTAAATTGTACAAAGACTATTTGTCTAGCTATTAAACCACCTTCCATTTTACTTATTTTAAGGACTATAAATGACTGATGTTCACAATTTCATGACTAAAACGACCGACATTTACAATTTCAAGAACCAAATTTGTATTTAAGTAAATGAATTTCAACACTTTGGTATCAACAAACTCCTGCTTGTAAACTTAACTAGTTTATCACCTCTGTAGGTTTGATGCACTAAGGTTTCTTGGAAAAATGAGAAGGAAGAGAATAATGTTGGTGGGTGATTCAATAATGAGAAATCAGTGGGAATCTTTAGTATGCTTAGTACAAGGTGTAATACCAACTGGTAGAAAAAGGGTGACCTACCATGGACCTGCAATGGCCTTCCATTCAATGGTAATGAAGACATTCATCAAACACATTTTCTTTTCTTCCTTTATAAATTATTGACACCACAATTTTCTCGGATAGGATTTTGAGACATCAATTGAGTTTCTTTGGGCACCACTGTTAGTGGAACTGAAGAAAAGTCCAGAAAATAAGAGAGTCTTACATTTGGATTTGATTGAGGAGAATGCAAAGTATTGGAGAGGAGTTGATATCCTTGTATTTGATTCAGCTCAGTGGTGGACTCACTCTGATAAAACAAGCTCGTAAGGAAGATTCTTCATCAATTAATATTCCACCTTCTCTTCATATCATTCCATTTTCTCAATTTCCCTCTTAGGACAAACTACTTAATTCAGCATTTATAGCATAAGCGTTTATCATTTAAGTAATTATGTAAAAGATATTTTTGCAACAAAAAATAAAATTAACTCAAATTGTTTTTATATAAGTTGTTTTCGTAAACTATCTTAGAAAACTTATGGGAATAAGCCAAAATCAGCTTATGGACATGGGATAAGTTGCTTTGATAAACTTTCTAAAAGAGGCTTAATCTTAAATTTTCAATAATGCTTCCCATTTATATGCTTCTTTCTTGACTAATACAGGTGGGATTACTACAAGGAGGGAAATAATCTGATCAAAAACATGACTCTTATGGTTGCTTATCAAAAAGGACTGAGTACATGGGCAAGGTGGGTGGATCTAAATCTAAACCCTAAAAAAACCAAAGTCATTTTCAGAAGCATGTCACCTAGGCATAACAGGTACTATATACACTTTTATATGCCTAAAACTTGTTTATCTTTGTCTATACAATAACGACCAAGTCTTATATCTCACTAAGTGAAGTTGATTACATGGATCAAACGACACCATAATATTTTATCGGAAATCATGTTTATAGCCAAACCAGCCATTGAGCTTCTAAATCTTAACAGTTTTTCGCCTATAGTTTTGTTTTTGGCATTTCATTGTCTCTAGCGATTGGATTTTTTATGTAGGGAAAATGGTTGGAAATGCGACAATCAGAAACAGCCTCTACCATTTTTCAGCCTGCAACAACATGCTCCTGAACCTTTAGTTGTTCTCCAAGGAGTTCTGAAGAAAATGAGGTTTCCGGTATACCTGCAAGATATTACGACAATGACTGCCTTACGCAGAGATGGACATCCATCAGTGTATAGGAAGGCAACCAGCCAAGATGAGAAACAAAGAATAGGCCATTCCTCTGATTGCAGTCATTGGTGCCTCCCTGGTGTGCCTGATATTTAG
Protein sequence

>MS.gene84006.t1

MATPMTHFLSFFLLFSFFHCTLSLLDPQDDVGFLVEPDYVNIVQNNRDSSKRCDFSVGKWVYDESYPLYDPNCPYLSTAVTCQKNGRPDSDYEKWKWKPSGCSIPRFDALRFLGKMRRKRIMLVGDSIMRNQWESLVCLVQGVIPTGRKRVTYHGPAMAFHSMDFETSIEFLWAPLLVELKKSPENKRVLHLDLIEENAKYWRGVDILVFDSAQWWTHSDKTSSWDYYKEGNNLIKNMTLMVAYQKGLSTWARWVDLNLNPKKTKVIFRSMSPRHNRENGWKCDNQKQPLPFFSLQQHAPEPLVVLQGVLKKMRFPVYLQDITTMTALRRDGHPSVYRKATSQDEKQRIGHSSDCSHWCLPGVPDI