Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84709.t1 | XP_003601914.2 | 87.4 | 246 | 25 | 2 | 1 | 242 | 1 | 244 | 3.80E-111 | 411 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84709.t1 | O22227 | 41.5 | 171 | 84 | 5 | 83 | 240 | 129 | 296 | 3.0e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84709.t1 | G7J372 | 87.4 | 246 | 25 | 2 | 1 | 242 | 1 | 244 | 2.8e-111 | 411.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene84709 | 0.810308 | 1.23E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene84709 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene84709 | 0.82416 | 9.38E-54 | -1.69E-46 |
| MS.gene050020 | MS.gene84709 | 0.822867 | 1.88E-53 | -1.69E-46 |
| MS.gene05016 | MS.gene84709 | 0.811397 | 7.15E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene84709 | 0.816886 | 4.39E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene84709 | 0.807901 | 4.03E-50 | -1.69E-46 |
| MS.gene050962 | MS.gene84709 | 0.804011 | 2.65E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene84709 | 0.827591 | 1.44E-54 | -1.69E-46 |
| MS.gene05157 | MS.gene84709 | 0.802061 | 6.71E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene84709 | 0.82247 | 2.33E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene84709 | 0.818952 | 1.50E-52 | -1.69E-46 |
| MS.gene052323 | MS.gene84709 | 0.810712 | 1.01E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene84709 | 0.811341 | 7.35E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene84709 | 0.820171 | 7.90E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene84709 | 0.803556 | 3.29E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene84709 | 0.825908 | 3.63E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene84709 | 0.803288 | 3.74E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene84709 | 0.813443 | 2.55E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene84709 | 0.808126 | 3.61E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene84709 | 0.803703 | 3.07E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene84709 | 0.803194 | 3.92E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene84709 | 0.812485 | 4.14E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene84709 | 0.804857 | 1.77E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene84709 | 0.819126 | 1.37E-52 | -1.69E-46 |
| MS.gene059607 | MS.gene84709 | 0.802211 | 6.25E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene84709 | 0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene060587 | MS.gene84709 | 0.818457 | 1.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84709.t1 | MTR_3g086740 | 96.341 | 246 | 3 | 2 | 1 | 242 | 1 | 244 | 2.17e-174 | 479 |
| MS.gene84709.t1 | MTR_8g089865 | 65.532 | 235 | 60 | 6 | 14 | 241 | 1 | 221 | 1.16e-96 | 282 |
| MS.gene84709.t1 | MTR_7g064810 | 47.239 | 163 | 80 | 3 | 83 | 242 | 65 | 224 | 8.32e-41 | 140 |
| MS.gene84709.t1 | MTR_8g040690 | 41.618 | 173 | 94 | 4 | 72 | 240 | 77 | 246 | 2.67e-40 | 139 |
| MS.gene84709.t1 | MTR_8g040680 | 43.210 | 162 | 85 | 4 | 83 | 240 | 98 | 256 | 8.51e-39 | 135 |
| MS.gene84709.t1 | MTR_3g101300 | 42.286 | 175 | 88 | 6 | 77 | 241 | 65 | 236 | 1.42e-37 | 132 |
| MS.gene84709.t1 | MTR_3g094830 | 40.719 | 167 | 91 | 5 | 79 | 240 | 95 | 258 | 5.71e-37 | 130 |
| MS.gene84709.t1 | MTR_4g112370 | 37.126 | 167 | 91 | 4 | 83 | 240 | 98 | 259 | 1.21e-33 | 122 |
| MS.gene84709.t1 | MTR_2g054380 | 38.547 | 179 | 92 | 6 | 72 | 240 | 99 | 269 | 1.54e-33 | 122 |
| MS.gene84709.t1 | MTR_1g053130 | 42.188 | 192 | 91 | 5 | 66 | 240 | 95 | 283 | 2.58e-32 | 119 |
| MS.gene84709.t1 | MTR_2g055990 | 36.207 | 174 | 102 | 4 | 73 | 240 | 88 | 258 | 6.52e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84709.t1 | AT5G65340 | 54.959 | 242 | 93 | 6 | 11 | 241 | 14 | 250 | 1.19e-82 | 248 |
| MS.gene84709.t1 | AT2G22460 | 58.442 | 231 | 85 | 5 | 18 | 241 | 17 | 243 | 1.24e-78 | 237 |
| MS.gene84709.t1 | AT1G21050 | 48.035 | 229 | 105 | 6 | 19 | 241 | 25 | 245 | 4.63e-60 | 189 |
| MS.gene84709.t1 | AT1G21050 | 48.035 | 229 | 105 | 6 | 19 | 241 | 61 | 281 | 1.12e-59 | 190 |
| MS.gene84709.t1 | AT1G76610 | 44.643 | 224 | 105 | 5 | 19 | 241 | 21 | 226 | 2.46e-51 | 167 |
| MS.gene84709.t1 | AT2G21990 | 44.253 | 174 | 90 | 4 | 71 | 240 | 81 | 251 | 4.11e-43 | 146 |
| MS.gene84709.t1 | AT4G39610 | 43.956 | 182 | 84 | 6 | 71 | 240 | 88 | 263 | 1.53e-42 | 145 |
| MS.gene84709.t1 | AT2G37880 | 44.970 | 169 | 83 | 4 | 81 | 241 | 79 | 245 | 3.61e-41 | 141 |
| MS.gene84709.t1 | AT5G06990 | 41.618 | 173 | 85 | 4 | 81 | 240 | 91 | 260 | 1.42e-38 | 135 |
| MS.gene84709.t1 | AT2G41660 | 41.520 | 171 | 84 | 5 | 83 | 240 | 129 | 296 | 8.57e-35 | 126 |
| MS.gene84709.t1 | AT5G42680 | 41.765 | 170 | 91 | 6 | 77 | 241 | 71 | 237 | 9.42e-35 | 124 |
| MS.gene84709.t1 | AT5G42680 | 41.765 | 170 | 91 | 6 | 77 | 241 | 71 | 237 | 9.42e-35 | 124 |
| MS.gene84709.t1 | AT3G25640 | 35.260 | 173 | 93 | 6 | 83 | 240 | 98 | 266 | 7.67e-30 | 112 |
| MS.gene84709.t1 | AT3G25640 | 35.260 | 173 | 93 | 6 | 83 | 240 | 124 | 292 | 1.62e-29 | 112 |
| MS.gene84709.t1 | AT5G23100 | 31.443 | 194 | 94 | 5 | 81 | 240 | 88 | 276 | 1.12e-25 | 102 |
Find 40 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGTACTGGTGTTATAAA+TGG | 0.255094 | 3.4:+80771335 | MS.gene84709:CDS |
| ACAATTACTGGCACGATATT+CGG | 0.320130 | 3.4:+80771032 | MS.gene84709:CDS |
| TCCTTTGCTAAAACACTTGT+TGG | 0.329615 | 3.4:-80771137 | None:intergenic |
| ATGGATATTTGTTATAGTTT+TGG | 0.334698 | 3.4:-80770980 | None:intergenic |
| GCAAGTTCTAAAAGAAGAAT+AGG | 0.336410 | 3.4:-80771110 | None:intergenic |
| GTCTTGTTCTTGTTCTACTA+AGG | 0.338250 | 3.4:-80770928 | None:intergenic |
| CAAGTTCTAAAAGAAGAATA+GGG | 0.363193 | 3.4:-80771109 | None:intergenic |
| TTTGGATTATGTATTGTAAT+GGG | 0.364697 | 3.4:+80771234 | MS.gene84709:CDS |
| ATGACATCTACAACAATTAC+TGG | 0.370669 | 3.4:+80771020 | MS.gene84709:CDS |
| CTTTGGATTATGTATTGTAA+TGG | 0.380120 | 3.4:+80771233 | MS.gene84709:CDS |
| TCTTGACCAATGCTTCCTTC+AGG | 0.407088 | 3.4:-80771464 | None:intergenic |
| GCTTCTAAATCACTTCTTGA+AGG | 0.409476 | 3.4:-80771287 | None:intergenic |
| CGGTCGGTTTCTGTTGGTAC+TGG | 0.430039 | 3.4:+80771323 | MS.gene84709:CDS |
| TAAAAGAAGAATAGGGTTTG+TGG | 0.440606 | 3.4:-80771102 | None:intergenic |
| TGGGAGGAAAGTCGGGTATT+CGG | 0.443287 | 3.4:+80771253 | MS.gene84709:CDS |
| CTAAGGTGCAGTTGCAAGTA+GGG | 0.450004 | 3.4:-80770911 | None:intergenic |
| ATTGTAATGGGAGGAAAGTC+GGG | 0.463638 | 3.4:+80771246 | MS.gene84709:CDS |
| TCTTAGAGCTAACTTTCAAA+GGG | 0.464488 | 3.4:+80771394 | MS.gene84709:CDS |
| ATCTTAGAGCTAACTTTCAA+AGG | 0.465935 | 3.4:+80771393 | MS.gene84709:CDS |
| TATTGTAATGGGAGGAAAGT+CGG | 0.482311 | 3.4:+80771245 | MS.gene84709:CDS |
| ATTGATCCTGAAGGAAGCAT+TGG | 0.506241 | 3.4:+80771458 | MS.gene84709:CDS |
| GCTAACTTTCAAAGGGTTCG+TGG | 0.509016 | 3.4:+80771401 | MS.gene84709:CDS |
| ACGATATTCGGATACCGTAA+AGG | 0.517456 | 3.4:+80771044 | MS.gene84709:CDS |
| ACTACCAACCTTGGTGATGT+AGG | 0.531843 | 3.4:-80770799 | None:intergenic |
| ATCCACTGTGGTTACACCAT+TGG | 0.548210 | 3.4:-80770832 | None:intergenic |
| TTGATGCGGTCGGTTTCTGT+TGG | 0.551122 | 3.4:+80771317 | MS.gene84709:CDS |
| ACTAAGGTGCAGTTGCAAGT+AGG | 0.551502 | 3.4:-80770912 | None:intergenic |
| ATGAGTGAAGAATGTGATGA+TGG | 0.567861 | 3.4:-80770999 | None:intergenic |
| GGATTATGTATTGTAATGGG+AGG | 0.573929 | 3.4:+80771237 | MS.gene84709:CDS |
| TAGAAGCACTTAACTTGATG+CGG | 0.577344 | 3.4:+80771303 | MS.gene84709:CDS |
| TTTCATTTGATTGATCCTGA+AGG | 0.587099 | 3.4:+80771449 | MS.gene84709:CDS |
| ATGGCCTACATCACCAAGGT+TGG | 0.591227 | 3.4:+80770795 | MS.gene84709:CDS |
| AGCACTTAACTTGATGCGGT+CGG | 0.611210 | 3.4:+80771307 | MS.gene84709:CDS |
| GGTAGTAGCAGCATGACCAA+TGG | 0.630024 | 3.4:+80770816 | MS.gene84709:CDS |
| CATGCTGCTACTACCAACCT+TGG | 0.631697 | 3.4:-80770808 | None:intergenic |
| TTACAATACATAATCCAAAG+AGG | 0.638246 | 3.4:-80771230 | None:intergenic |
| TTAGCAAAGGAAATGAGAGG+AGG | 0.650662 | 3.4:+80771149 | MS.gene84709:CDS |
| GTACCATAAATATATGACAA+TGG | 0.651023 | 3.4:+80770776 | None:intergenic |
| GACAATGGCCTACATCACCA+AGG | 0.711681 | 3.4:+80770791 | MS.gene84709:CDS |
| GACCAATGGTGTAACCACAG+TGG | 0.740457 | 3.4:+80770830 | MS.gene84709:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGGATTATGTATTGTAAT+GGG | + | chr3.4:80771234-80771253 | MS.gene84709:CDS | 20.0% |
| !!! | AAAAACTTACTTTTCCTTTA+CGG | - | chr3.4:80771061-80771080 | None:intergenic | 20.0% |
| !!! | ATGGATATTTGTTATAGTTT+TGG | - | chr3.4:80770983-80771002 | None:intergenic | 20.0% |
| ! | CTTTGGATTATGTATTGTAA+TGG | + | chr3.4:80771233-80771252 | MS.gene84709:CDS | 25.0% |
| ! | TTACAATACATAATCCAAAG+AGG | - | chr3.4:80771233-80771252 | None:intergenic | 25.0% |
| !!! | CAAGTTCTAAAAGAAGAATA+GGG | - | chr3.4:80771112-80771131 | None:intergenic | 25.0% |
| !!! | GTTTTGAAATGCTAATATCT+TGG | - | chr3.4:80770953-80770972 | None:intergenic | 25.0% |
| ATCTTAGAGCTAACTTTCAA+AGG | + | chr3.4:80771393-80771412 | MS.gene84709:CDS | 30.0% | |
| ATGACATCTACAACAATTAC+TGG | + | chr3.4:80771020-80771039 | MS.gene84709:CDS | 30.0% | |
| TAAAAGAAGAATAGGGTTTG+TGG | - | chr3.4:80771105-80771124 | None:intergenic | 30.0% | |
| TCTTAGAGCTAACTTTCAAA+GGG | + | chr3.4:80771394-80771413 | MS.gene84709:CDS | 30.0% | |
| TTTCATTTGATTGATCCTGA+AGG | + | chr3.4:80771449-80771468 | MS.gene84709:CDS | 30.0% | |
| !! | GCAAGTTCTAAAAGAAGAAT+AGG | - | chr3.4:80771113-80771132 | None:intergenic | 30.0% |
| AAAAACAAGTTCGATCATGG+AGG | + | chr3.4:80770859-80770878 | MS.gene84709:CDS | 35.0% | |
| ACAATTACTGGCACGATATT+CGG | + | chr3.4:80771032-80771051 | MS.gene84709:CDS | 35.0% | |
| ATGAGTGAAGAATGTGATGA+TGG | - | chr3.4:80771002-80771021 | None:intergenic | 35.0% | |
| GCTTCTAAATCACTTCTTGA+AGG | - | chr3.4:80771290-80771309 | None:intergenic | 35.0% | |
| GTCAAAAACAAGTTCGATCA+TGG | + | chr3.4:80770856-80770875 | MS.gene84709:CDS | 35.0% | |
| TCCTTTGCTAAAACACTTGT+TGG | - | chr3.4:80771140-80771159 | None:intergenic | 35.0% | |
| ! | GGATTATGTATTGTAATGGG+AGG | + | chr3.4:80771237-80771256 | MS.gene84709:CDS | 35.0% |
| ! | GTCTTGTTCTTGTTCTACTA+AGG | - | chr3.4:80770931-80770950 | None:intergenic | 35.0% |
| ! | TATTGTAATGGGAGGAAAGT+CGG | + | chr3.4:80771245-80771264 | MS.gene84709:CDS | 35.0% |
| !! | ACTTGTTTTCAACTCCTCTT+TGG | + | chr3.4:80771216-80771235 | MS.gene84709:CDS | 35.0% |
| !! | GTTGGTACTGGTGTTATAAA+TGG | + | chr3.4:80771335-80771354 | MS.gene84709:CDS | 35.0% |
| !! | GTTTTAGCAAAGGAAATGAG+AGG | + | chr3.4:80771146-80771165 | MS.gene84709:CDS | 35.0% |
| !! | TAGAAGCACTTAACTTGATG+CGG | + | chr3.4:80771303-80771322 | MS.gene84709:CDS | 35.0% |
| !!! | TCCAACAAGTGTTTTAGCAA+AGG | + | chr3.4:80771136-80771155 | MS.gene84709:CDS | 35.0% |
| !!! | TTGTTTTTGACAATCCACTG+TGG | - | chr3.4:80770847-80770866 | None:intergenic | 35.0% |
| ACGATATTCGGATACCGTAA+AGG | + | chr3.4:80771044-80771063 | MS.gene84709:CDS | 40.0% | |
| TTAGCAAAGGAAATGAGAGG+AGG | + | chr3.4:80771149-80771168 | MS.gene84709:CDS | 40.0% | |
| ! | ATTGATCCTGAAGGAAGCAT+TGG | + | chr3.4:80771458-80771477 | MS.gene84709:CDS | 40.0% |
| ! | ATTGTAATGGGAGGAAAGTC+GGG | + | chr3.4:80771246-80771265 | MS.gene84709:CDS | 40.0% |
| ACTAAGGTGCAGTTGCAAGT+AGG | - | chr3.4:80770915-80770934 | None:intergenic | 45.0% | |
| ATCCACTGTGGTTACACCAT+TGG | - | chr3.4:80770835-80770854 | None:intergenic | 45.0% | |
| CTAAGGTGCAGTTGCAAGTA+GGG | - | chr3.4:80770914-80770933 | None:intergenic | 45.0% | |
| GCTAACTTTCAAAGGGTTCG+TGG | + | chr3.4:80771401-80771420 | MS.gene84709:CDS | 45.0% | |
| TCTTGACCAATGCTTCCTTC+AGG | - | chr3.4:80771467-80771486 | None:intergenic | 45.0% | |
| ! | AGCACTTAACTTGATGCGGT+CGG | + | chr3.4:80771307-80771326 | MS.gene84709:CDS | 45.0% |
| !! | ACTACCAACCTTGGTGATGT+AGG | - | chr3.4:80770802-80770821 | None:intergenic | 45.0% |
| ATGGCCTACATCACCAAGGT+TGG | + | chr3.4:80770795-80770814 | MS.gene84709:CDS | 50.0% | |
| CATGCTGCTACTACCAACCT+TGG | - | chr3.4:80770811-80770830 | None:intergenic | 50.0% | |
| GACAATGGCCTACATCACCA+AGG | + | chr3.4:80770791-80770810 | MS.gene84709:CDS | 50.0% | |
| GACCAATGGTGTAACCACAG+TGG | + | chr3.4:80770830-80770849 | MS.gene84709:CDS | 50.0% | |
| GGTAGTAGCAGCATGACCAA+TGG | + | chr3.4:80770816-80770835 | MS.gene84709:CDS | 50.0% | |
| TGGGAGGAAAGTCGGGTATT+CGG | + | chr3.4:80771253-80771272 | MS.gene84709:CDS | 50.0% | |
| ! | TTGATGCGGTCGGTTTCTGT+TGG | + | chr3.4:80771317-80771336 | MS.gene84709:CDS | 50.0% |
| ! | CGGTCGGTTTCTGTTGGTAC+TGG | + | chr3.4:80771323-80771342 | MS.gene84709:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 80770789 | 80771517 | 80770789 | ID=MS.gene84709 |
| chr3.4 | mRNA | 80770789 | 80771517 | 80770789 | ID=MS.gene84709.t1;Parent=MS.gene84709 |
| chr3.4 | exon | 80770789 | 80771517 | 80770789 | ID=MS.gene84709.t1.exon1;Parent=MS.gene84709.t1 |
| chr3.4 | CDS | 80770789 | 80771517 | 80770789 | ID=cds.MS.gene84709.t1;Parent=MS.gene84709.t1 |
| Gene Sequence |
| Protein sequence |
