Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86931.t1 | XP_013469974.1 | 85.7 | 196 | 23 | 4 | 1 | 191 | 3 | 198 | 3.40E-86 | 327.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86931.t1 | A0A072VQE3 | 85.7 | 196 | 23 | 4 | 1 | 191 | 3 | 198 | 2.4e-86 | 327.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene86931 | 0.80714 | 5.84E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene86931 | 0.835383 | 1.73E-56 | -1.69E-46 |
| MS.gene050384 | MS.gene86931 | 0.801862 | 7.37E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene86931 | 0.817814 | 2.71E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene86931 | 0.812085 | 5.06E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene86931 | 0.82945 | 5.11E-55 | -1.69E-46 |
| MS.gene050901 | MS.gene86931 | 0.823576 | 1.29E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene86931 | 0.815306 | 9.89E-52 | -1.69E-46 |
| MS.gene051328 | MS.gene86931 | 0.802757 | 4.82E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene86931 | 0.852427 | 4.65E-61 | -1.69E-46 |
| MS.gene051720 | MS.gene86931 | 0.844553 | 7.04E-59 | -1.69E-46 |
| MS.gene051902 | MS.gene86931 | 0.814944 | 1.19E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene86931 | 0.809495 | 1.84E-50 | -1.69E-46 |
| MS.gene052335 | MS.gene86931 | 0.822209 | 2.68E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene86931 | 0.824597 | 7.40E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene86931 | 0.824368 | 8.38E-54 | -1.69E-46 |
| MS.gene052665 | MS.gene86931 | 0.807958 | 3.92E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene86931 | 0.872893 | 2.24E-67 | -1.69E-46 |
| MS.gene05364 | MS.gene86931 | 0.80599 | 1.02E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene86931 | 0.826802 | 2.22E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene86931 | 0.823706 | 1.20E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene86931 | 0.83413 | 3.58E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene86931 | 0.812757 | 3.61E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene86931 | 0.811203 | 7.87E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene86931 | 0.801967 | 7.01E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene86931 | 0.804898 | 1.73E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene86931 | 0.820484 | 6.69E-53 | -1.69E-46 |
| MS.gene056390 | MS.gene86931 | 0.810411 | 1.17E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene86931 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene86931 | 0.809513 | 1.82E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene86931 | 0.814292 | 1.66E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene86931 | 0.80581 | 1.12E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene86931 | 0.820176 | 7.88E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86931.t1 | MTR_1g106080 | 92.347 | 196 | 10 | 4 | 1 | 191 | 3 | 198 | 7.90e-119 | 335 |
| MS.gene86931.t1 | MTR_5g005230 | 49.215 | 191 | 82 | 9 | 1 | 187 | 1 | 180 | 1.19e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86931.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.40e-20 | 84.3 |
| MS.gene86931.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.40e-20 | 84.3 |
Find 28 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.266605 | 1.2:-79073162 | MS.gene86931:CDS |
| TCATACCAGGAAGAACAATT+TGG | 0.315479 | 1.2:-79072645 | MS.gene86931:CDS |
| ACAAAGTTATGTAGGAAATA+TGG | 0.330154 | 1.2:-79072807 | MS.gene86931:CDS |
| TATGAAAACACTAGATTCAT+AGG | 0.343147 | 1.2:-79073267 | MS.gene86931:CDS |
| TATTTCCTACATAACTTTGT+TGG | 0.375448 | 1.2:+79072810 | None:intergenic |
| TGTAGTAGTGGATGTGAGTC+AGG | 0.376527 | 1.2:-79073315 | MS.gene86931:CDS |
| AGGAGTGGAAGATTTGTCTA+TGG | 0.397750 | 1.2:-79073181 | MS.gene86931:CDS |
| AAATTGTTCTTCCTGGTATG+AGG | 0.406008 | 1.2:+79072647 | None:intergenic |
| TCTTTGTTTAGCAGCTGGTA+TGG | 0.422441 | 1.2:-79072490 | MS.gene86931:intron |
| GGTGGAACTGAAGGGTACTA+TGG | 0.434994 | 1.2:-79073246 | MS.gene86931:CDS |
| GCAGTGTCATGAAGAAAAGA+AGG | 0.507146 | 1.2:+79073006 | None:intergenic |
| ACAAACCAACAAAGTTATGT+AGG | 0.515352 | 1.2:-79072815 | MS.gene86931:CDS |
| TTGGTTTGTCCCAACAACAT+TGG | 0.519643 | 1.2:+79072829 | None:intergenic |
| GGTACAACGAAACAGAAAGT+TGG | 0.538699 | 1.2:-79073069 | MS.gene86931:CDS |
| ACATCATCATGTGGAAGATG+TGG | 0.558107 | 1.2:+79073135 | None:intergenic |
| AGATTCATAGGTGGAACTGA+AGG | 0.568350 | 1.2:-79073255 | MS.gene86931:CDS |
| GTCACAAATGAGTATTCTGA+AGG | 0.574749 | 1.2:-79073201 | MS.gene86931:CDS |
| AAATGAGTATTCTGAAGGAG+TGG | 0.595088 | 1.2:-79073196 | MS.gene86931:CDS |
| TTGTTTAGCAGCTGGTATGG+AGG | 0.596105 | 1.2:-79072487 | MS.gene86931:intron |
| TTTGATTCTGAATGTAGTAG+TGG | 0.600879 | 1.2:-79073327 | MS.gene86931:CDS |
| GTAGGGAAAATCCTCATACC+AGG | 0.622430 | 1.2:-79072658 | MS.gene86931:intron |
| TCATCATGTGGAAGATGTGG+AGG | 0.636849 | 1.2:+79073138 | None:intergenic |
| TTGAAGTAAACATCATCATG+TGG | 0.637487 | 1.2:+79073126 | None:intergenic |
| GATTCATAGGTGGAACTGAA+GGG | 0.638366 | 1.2:-79073254 | MS.gene86931:CDS |
| GTAGTGGATGTGAGTCAGGT+TGG | 0.642732 | 1.2:-79073311 | MS.gene86931:CDS |
| TGTCATGAAGAAAAGAAGGA+AGG | 0.667075 | 1.2:+79073010 | None:intergenic |
| GAAAACACTAGATTCATAGG+TGG | 0.672436 | 1.2:-79073264 | MS.gene86931:CDS |
| AATATGGTAGACTATTCACA+AGG | 0.724521 | 1.2:-79072791 | MS.gene86931:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGTTTGTGTTATTTGT+AGG | - | chr1.2:79073128-79073147 | MS.gene86931:CDS | 20.0% |
| !!! | ACTAATTATTTTGAGGTAAT+TGG | - | chr1.2:79073046-79073065 | MS.gene86931:CDS | 20.0% |
| !!! | ATTTGTTTGTGTTATTTGTA+GGG | - | chr1.2:79073129-79073148 | MS.gene86931:CDS | 20.0% |
| ! | ACAAAGTTATGTAGGAAATA+TGG | - | chr1.2:79072997-79073016 | MS.gene86931:CDS | 25.0% |
| ! | TATGAAAACACTAGATTCAT+AGG | - | chr1.2:79072537-79072556 | MS.gene86931:intron | 25.0% |
| ! | TATTTCCTACATAACTTTGT+TGG | + | chr1.2:79072997-79073016 | None:intergenic | 25.0% |
| !! | CTTTTCAACTGTAAGAAATA+TGG | - | chr1.2:79072839-79072858 | MS.gene86931:intron | 25.0% |
| !! | TGTTTTCATAAGAATGTTCA+AGG | + | chr1.2:79072527-79072546 | None:intergenic | 25.0% |
| !!! | TTCAGCAACTAATTATTTTG+AGG | - | chr1.2:79073039-79073058 | MS.gene86931:CDS | 25.0% |
| AAAAACCAAATTGTTCTTCC+TGG | + | chr1.2:79073167-79073186 | None:intergenic | 30.0% | |
| AATATGGTAGACTATTCACA+AGG | - | chr1.2:79073013-79073032 | MS.gene86931:CDS | 30.0% | |
| ACAAACCAACAAAGTTATGT+AGG | - | chr1.2:79072989-79073008 | MS.gene86931:CDS | 30.0% | |
| CTCTCAAAAAATGAACTTCA+AGG | - | chr1.2:79073198-79073217 | MS.gene86931:CDS | 30.0% | |
| TTGAAGTAAACATCATCATG+TGG | + | chr1.2:79072681-79072700 | None:intergenic | 30.0% | |
| TTTGATTCTGAATGTAGTAG+TGG | - | chr1.2:79072477-79072496 | MS.gene86931:CDS | 30.0% | |
| !! | ATTTTTCAGACCAATGTTGT+TGG | - | chr1.2:79072965-79072984 | MS.gene86931:intron | 30.0% |
| !! | TTTTTCAGACCAATGTTGTT+GGG | - | chr1.2:79072966-79072985 | MS.gene86931:intron | 30.0% |
| !!! | AGTTCATTTTTTGAGAGAGA+TGG | + | chr1.2:79073195-79073214 | None:intergenic | 30.0% |
| AAATTGTTCTTCCTGGTATG+AGG | + | chr1.2:79073160-79073179 | None:intergenic | 35.0% | |
| GAAAACACTAGATTCATAGG+TGG | - | chr1.2:79072540-79072559 | MS.gene86931:intron | 35.0% | |
| GTCACAAATGAGTATTCTGA+AGG | - | chr1.2:79072603-79072622 | MS.gene86931:CDS | 35.0% | |
| TCATACCAGGAAGAACAATT+TGG | - | chr1.2:79073159-79073178 | MS.gene86931:CDS | 35.0% | |
| TGTCATGAAGAAAAGAAGGA+AGG | + | chr1.2:79072797-79072816 | None:intergenic | 35.0% | |
| ! | AAATGAGTATTCTGAAGGAG+TGG | - | chr1.2:79072608-79072627 | MS.gene86931:CDS | 35.0% |
| ! | GTAGTGAAAAAGCACAAGAA+AGG | - | chr1.2:79072714-79072733 | MS.gene86931:intron | 35.0% |
| ! | TGACTCTTTTGTCTTTGTGT+TGG | + | chr1.2:79072587-79072606 | None:intergenic | 35.0% |
| ! | TTCATTCTTTGTTTAGCAGC+TGG | - | chr1.2:79073309-79073328 | MS.gene86931:CDS | 35.0% |
| ACATCATCATGTGGAAGATG+TGG | + | chr1.2:79072672-79072691 | None:intergenic | 40.0% | |
| AGATTCATAGGTGGAACTGA+AGG | - | chr1.2:79072549-79072568 | MS.gene86931:intron | 40.0% | |
| AGGAGTGGAAGATTTGTCTA+TGG | - | chr1.2:79072623-79072642 | MS.gene86931:CDS | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | - | chr1.2:79072642-79072661 | MS.gene86931:CDS | 40.0% | |
| GATTCATAGGTGGAACTGAA+GGG | - | chr1.2:79072550-79072569 | MS.gene86931:intron | 40.0% | |
| GCAGTGTCATGAAGAAAAGA+AGG | + | chr1.2:79072801-79072820 | None:intergenic | 40.0% | |
| GGTACAACGAAACAGAAAGT+TGG | - | chr1.2:79072735-79072754 | MS.gene86931:intron | 40.0% | |
| ! | TCTTTGTTTAGCAGCTGGTA+TGG | - | chr1.2:79073314-79073333 | MS.gene86931:CDS | 40.0% |
| !! | TTGGTTTGTCCCAACAACAT+TGG | + | chr1.2:79072978-79072997 | None:intergenic | 40.0% |
| GTAGGGAAAATCCTCATACC+AGG | - | chr1.2:79073146-79073165 | MS.gene86931:CDS | 45.0% | |
| TCATCATGTGGAAGATGTGG+AGG | + | chr1.2:79072669-79072688 | None:intergenic | 45.0% | |
| TTGTTTAGCAGCTGGTATGG+AGG | - | chr1.2:79073317-79073336 | MS.gene86931:CDS | 45.0% | |
| !! | TGTAGTAGTGGATGTGAGTC+AGG | - | chr1.2:79072489-79072508 | MS.gene86931:intron | 45.0% |
| !! | GGTGGAACTGAAGGGTACTA+TGG | - | chr1.2:79072558-79072577 | MS.gene86931:intron | 50.0% |
| !! | GTAGTGGATGTGAGTCAGGT+TGG | - | chr1.2:79072493-79072512 | MS.gene86931:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 79072468 | 79073358 | 79072468 | ID=MS.gene86931 |
| chr1.2 | mRNA | 79072468 | 79073358 | 79072468 | ID=MS.gene86931.t1;Parent=MS.gene86931 |
| chr1.2 | exon | 79072978 | 79073358 | 79072978 | ID=MS.gene86931.t1.exon1;Parent=MS.gene86931.t1 |
| chr1.2 | CDS | 79072978 | 79073358 | 79072978 | ID=cds.MS.gene86931.t1;Parent=MS.gene86931.t1 |
| chr1.2 | exon | 79072766 | 79072852 | 79072766 | ID=MS.gene86931.t1.exon2;Parent=MS.gene86931.t1 |
| chr1.2 | CDS | 79072766 | 79072852 | 79072766 | ID=cds.MS.gene86931.t1;Parent=MS.gene86931.t1 |
| chr1.2 | exon | 79072600 | 79072676 | 79072600 | ID=MS.gene86931.t1.exon3;Parent=MS.gene86931.t1 |
| chr1.2 | CDS | 79072600 | 79072676 | 79072600 | ID=cds.MS.gene86931.t1;Parent=MS.gene86931.t1 |
| chr1.2 | exon | 79072468 | 79072498 | 79072468 | ID=MS.gene86931.t1.exon4;Parent=MS.gene86931.t1 |
| chr1.2 | CDS | 79072468 | 79072498 | 79072468 | ID=cds.MS.gene86931.t1;Parent=MS.gene86931.t1 |
| Gene Sequence |
| Protein sequence |
